Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 288,249 T→A 100% T137T (ACA→ACT)  yagK ← CP4‑6 prophage; conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273288,2490TA99.3% 694.0 / ‑4.5 206T137T (ACA→ACT) yagKCP4‑6 prophage; conserved protein
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base A (83/121);  total (85/121)
Fisher's exact test for biased strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 206 total aligned reads displayed.

GGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCAACGGTA  >  CP009273/288104‑288395
                                                                                                                                                 |                                                                                                                                                  
ggTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGa                                                                                                                                                >  2:4476329/1‑150 (MQ=255)
 gTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGat                                                                                                                                               <  2:2076778/150‑1 (MQ=255)
   aGTCCAGTAGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGatca                                                                                                                                             <  2:1994221/150‑1 (MQ=255)
    gTCCAGTCGGGTTAACAGCACCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGatcat                                                                                                                                            <  2:2582280/150‑1 (MQ=255)
      ccAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATcc                                                                                                                                          >  2:223885/1‑150 (MQ=255)
      ccAGTCGGGTTAACAGCGCCTGGTAGTTCGGCTGGAAGTCAGTACTAGTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATcc                                                                                                                                          >  2:2553159/1‑150 (MQ=255)
          tCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCctct                                                                                                                                      <  2:4802436/150‑1 (MQ=255)
          tCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCctct                                                                                                                                      >  2:4780009/1‑150 (MQ=255)
            gggTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTaa                                                                                                                                    <  2:590970/150‑1 (MQ=255)
               ttAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTCAGGt                                                                                                                                 >  2:4338306/1‑150 (MQ=255)
                 aaCAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTTTTGGTGTCTAGCACATACTTACTGTTTTCAGGAAAATGGAGCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGATGTACCAGTCTCCAGTGATCATCCCTCTAATGTTa                                                                                                                               <  2:2742243/150‑1 (MQ=255)
                  aCAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTAt                                                                                                                              <  1:2611431/150‑1 (MQ=255)
                     gcgcCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGt                                                                                                                           >  1:486051/1‑150 (MQ=255)
                      cgcCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTc                                                                                                                          <  2:116422/150‑1 (MQ=255)
                      cgcCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTc                                                                                                                          <  2:4536457/150‑1 (MQ=255)
                           ggTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGAt                                                                                                                     <  2:304796/150‑1 (MQ=255)
                           ggTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGAt                                                                                                                     <  2:2499846/150‑1 (MQ=255)
                           ggTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGAt                                                                                                                     <  2:4966428/150‑1 (MQ=255)
                           ggTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGAt                                                                                                                     >  2:1380104/1‑150 (MQ=255)
                               gTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTa                                                                                                                 >  2:1858927/1‑150 (MQ=255)
                                    gctgGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATccc                                                                                                            <  2:638467/150‑1 (MQ=255)
                                      tgGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCg                                                                                                          <  2:1454044/150‑1 (MQ=255)
                                      tgGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCg                                                                                                          <  2:4059372/150‑1 (MQ=255)
                                      tgGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCg                                                                                                          <  1:3253000/150‑1 (MQ=255)
                                       ggAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGa                                                                                                         >  1:4343479/1‑150 (MQ=255)
                                        gAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATAGTAATCCCCGAg                                                                                                        <  1:2327593/150‑1 (MQ=255)
                                          aGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAggt                                                                                                      >  2:3702309/1‑150 (MQ=255)
                                           gTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAggtg                                                                                                     >  1:1310206/1‑150 (MQ=255)
                                            tCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAggtgg                                                                                                    >  2:4979477/1‑150 (MQ=255)
                                             cAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAggtggt                                                                                                   <  2:17140/150‑1 (MQ=255)
                                              aGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGgta                                                                                                  >  2:269897/1‑150 (MQ=255)
                                               gTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCCGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGgtag                                                                                                 <  2:802931/150‑1 (MQ=255)
                                                  cTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTaa                                                                                              <  1:3543321/150‑1 (MQ=255)
                                                  cTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTaa                                                                                              <  1:963109/150‑1 (MQ=255)
                                                  cTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTaa                                                                                              <  1:3456504/150‑1 (MQ=255)
                                                     ttGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCg                                                                                           >  1:2841092/1‑150 (MQ=255)
                                                     ttGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCg                                                                                           <  1:125321/150‑1 (MQ=255)
                                                     ttGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCg                                                                                           <  1:4416619/150‑1 (MQ=255)
                                                      tGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGt                                                                                          >  1:2159695/1‑150 (MQ=255)
                                                      tGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGt                                                                                          >  1:3692941/1‑150 (MQ=255)
                                                          gtCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCttt                                                                                      <  1:396117/150‑1 (MQ=255)
                                                            cTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTttgt                                                                                    <  1:1052135/150‑1 (MQ=255)
                                                                cacaTACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGaa                                                                                >  1:3152342/1‑150 (MQ=255)
                                                                      cTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAg                                                                          <  1:4866659/150‑1 (MQ=255)
                                                                        tACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGc                                                                        <  1:2566759/150‑1 (MQ=255)
                                                                        tACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGc                                                                        <  1:2516241/150‑1 (MQ=255)
                                                                          cAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCaa                                                                      <  2:4590683/150‑1 (MQ=255)
                                                                           aGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCaaa                                                                     >  1:2664388/1‑150 (MQ=255)
                                                                              tttCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAAtgt                                                                  <  2:4794927/150‑1 (MQ=255)
                                                                               ttCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAAtgtg                                                                 <  1:4682221/150‑1 (MQ=255)
                                                                                   ggAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAg                                                             <  2:1756577/150‑1 (MQ=255)
                                                                                   ggAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAg                                                             >  2:1277146/1‑150 (MQ=255)
                                                                                   ggAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAg                                                             <  2:3956511/150‑1 (MQ=255)
                                                                                    gAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGt                                                            >  1:4120951/1‑150 (MQ=255)
                                                                                    gAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGt                                                            <  2:1945539/150‑1 (MQ=255)
                                                                                     aaaaTGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTg                                                           <  2:3792732/150‑1 (MQ=255)
                                                                                      aaaTGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTgg                                                          <  1:2539637/150‑1 (MQ=255)
                                                                                       aaTGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGc                                                         >  2:3552671/1‑150 (MQ=255)
                                                                                          ggACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCAtt                                                      >  1:311606/1‑150 (MQ=255)
                                                                                           gaccagaccaGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCAttt                                                     <  2:486119/150‑1 (MQ=255)
                                                                                            accagaccagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTa                                                    <  2:1830652/150‑1 (MQ=255)
                                                                                              cagaccagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTAcc                                                  >  1:3855263/1‑150 (MQ=255)
                                                                                              cagaccagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTAcc                                                  <  2:2874534/150‑1 (MQ=255)
                                                                                               agaccagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCt                                                 >  1:555093/1‑150 (MQ=255)
                                                                                               agaccagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCt                                                 >  1:4290336/1‑150 (MQ=255)
                                                                                                gaccagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTg                                                <  2:3257214/150‑1 (MQ=255)
                                                                                                gaccagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTg                                                <  2:1646995/150‑1 (MQ=255)
                                                                                                  ccagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGAt                                              <  1:634531/150‑1 (MQ=255)
                                                                                                   cagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGAtt                                             <  1:1730728/150‑1 (MQ=255)
                                                                                                   cagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGAtt                                             <  2:2643626/150‑1 (MQ=255)
                                                                                                    agGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTc                                            >  1:992323/1‑150 (MQ=255)
                                                                                                     gggTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCa                                           <  1:1124743/150‑1 (MQ=255)
                                                                                                     gggTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCa                                           <  2:2116531/150‑1 (MQ=255)
                                                                                                       gTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGa                                         <  2:4905527/150‑1 (MQ=255)
                                                                                                       gTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGa                                         >  2:2188676/1‑150 (MQ=255)
                                                                                                            cGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATAct                                    <  2:3128179/150‑1 (MQ=255)
                                                                                                               ctctctcCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCtt                                 <  1:5013353/150‑1 (MQ=255)
                                                                                                                 ctctcCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTc                               <  1:2243821/150‑1 (MQ=255)
                                                                                                                 ctctcCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTc                               <  2:2793371/150‑1 (MQ=255)
                                                                                                                  tctcCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCg                              <  1:1547408/150‑1 (MQ=255)
                                                                                                                  tctcCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCg                              <  2:4482056/150‑1 (MQ=255)
                                                                                                                     ccAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGccc                           <  1:1944864/150‑1 (MQ=255)
                                                                                                                      cAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCa                          >  2:4021094/1‑150 (MQ=255)
                                                                                                                       aGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCat                         <  2:1285690/150‑1 (MQ=255)
                                                                                                                        gTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCata                        >  1:2930744/1‑150 (MQ=255)
                                                                                                                        gTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCata                        <  1:1643156/150‑1 (MQ=255)
                                                                                                                        gTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCata                        <  1:4626972/150‑1 (MQ=255)
                                                                                                                         tctcAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCatat                       <  1:2781350/150‑1 (MQ=255)
                                                                                                                         tctcAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCatat                       <  1:4476329/150‑1 (MQ=255)
                                                                                                                          ctcAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCAtata                      <  2:4137316/150‑1 (MQ=255)
                                                                                                                          ctcAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCAtata                      >  1:3091153/1‑150 (MQ=255)
                                                                                                                          ctcAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCAtata                      <  1:2427154/150‑1 (MQ=255)
                                                                                                                          ctcAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCAtata                      >  1:4694355/1‑150 (MQ=255)
                                                                                                                            cAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATataat                    <  1:4780009/150‑1 (MQ=255)
                                                                                                                             aGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATataata                   <  1:4400767/150‑1 (MQ=255)
                                                                                                                              gggCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATAtaataa                  >  1:2047761/1‑150 (MQ=255)
                                                                                                                                       aCCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGc         <  2:3252159/150‑1 (MQ=255)
                                                                                                                                          aGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCAAc      <  2:2129731/150‑1 (MQ=255)
                                                                                                                                            gCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCAACgg    >  1:3325353/1‑150 (MQ=255)
                                                                                                                                              tCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCAACGGTa  <  1:3010613/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCAACGGTA  >  CP009273/288104‑288395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCAACGGTAAAT  >  CP009273/288105‑288398
                                                                                                                                                |                                                                                                                                                     
GTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGAT                                                                                                                                                  <  SRR19973063.1876381/150‑1 (MQ=60)
 TAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATC                                                                                                                                                 <  SRR19973063.1089090/150‑1 (MQ=60)
  AGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCA                                                                                                                                                >  SRR19973063.2885216/1‑150 (MQ=60)
  AGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGAACAGGGTGATCGTATCTCTCCAGTCTCAGGGCGATGTAACAGGCTCCAGTGATCA                                                                                                                                                >  SRR19973063.2626477/1‑150 (MQ=60)
        GTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTC                                                                                                                                          >  SRR19973063.4810243/1‑150 (MQ=60)
        GTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTC                                                                                                                                          >  SRR19973063.1573458/1‑150 (MQ=60)
             GTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGG                                                                                                                                     >  SRR19973063.4004743/1‑150 (MQ=60)
                 ACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTAT                                                                                                                                 <  SRR19973063.4471808/150‑1 (MQ=60)
                 ACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTAT                                                                                                                                 <  SRR19973063.2619739/150‑1 (MQ=60)
                  CAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATC                                                                                                                                >  SRR19973063.2571134/1‑150 (MQ=60)
                    GCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGT                                                                                                                              >  SRR19973063.487439/1‑150 (MQ=60)
                    GCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGT                                                                                                                              >  SRR19973063.487508/1‑150 (MQ=60)
                         TGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGA                                                                                                                         >  SRR19973063.2315369/1‑150 (MQ=60)
                           GTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATC                                                                                                                       <  SRR19973063.2701665/150‑1 (MQ=60)
                           GTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATC                                                                                                                       <  SRR19973063.2970619/150‑1 (MQ=60)
                             AGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGT                                                                                                                     >  SRR19973063.3138249/1‑150 (MQ=60)
                                  TGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCC                                                                                                                <  SRR19973063.4716119/150‑1 (MQ=60)
                                  TGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCC                                                                                                                >  SRR19973063.3905751/1‑150 (MQ=60)
                                    CTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGGACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCC                                                                                                              <  SRR19973063.2392334/150‑1 (MQ=60)
                                     TGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCG                                                                                                             <  SRR19973063.3263438/150‑1 (MQ=60)
                                      GGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGA                                                                                                            >  SRR19973063.4357341/1‑150 (MQ=60)
                                      GGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGA                                                                                                            <  SRR19973063.975893/150‑1 (MQ=60)
                                       GAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATAGTAATCCCCGAG                                                                                                           <  SRR19973063.2335044/150‑1 (MQ=60)
                                         AGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGT                                                                                                         <  SRR19973063.4705823/150‑1 (MQ=60)
                                          GTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTG                                                                                                        >  SRR19973063.1314110/1‑150 (MQ=60)
                                            CAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGT                                                                                                      >  SRR19973063.2082698/1‑150 (MQ=60)
                                                 CTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAA                                                                                                 <  SRR19973063.966010/150‑1 (MQ=60)
                                                 CTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAA                                                                                                 <  SRR19973063.3554674/150‑1 (MQ=60)
                                                 CTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAA                                                                                                 <  SRR19973063.3467491/150‑1 (MQ=60)
                                                  TATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAG                                                                                                >  SRR19973063.4918553/1‑150 (MQ=60)
                                                   ATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGC                                                                                               >  SRR19973063.4665026/1‑150 (MQ=60)
                                                   ATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGC                                                                                               >  SRR19973063.1266988/1‑150 (MQ=60)
                                                    TTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCG                                                                                              >  SRR19973063.2850206/1‑150 (MQ=60)
                                                    TTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCG                                                                                              <  SRR19973063.125729/150‑1 (MQ=60)
                                                    TTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCG                                                                                              <  SRR19973063.4430798/150‑1 (MQ=60)
                                                     TGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGT                                                                                             <  SRR19973063.1483140/150‑1 (MQ=60)
                                                     TGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGT                                                                                             >  SRR19973063.3704764/1‑150 (MQ=60)
                                                     TGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGT                                                                                             >  SRR19973063.2166459/1‑150 (MQ=60)
                                                      GGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTC                                                                                            >  SRR19973063.435387/1‑150 (MQ=60)
                                                         GTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTT                                                                                         <  SRR19973063.397244/150‑1 (MQ=60)
                                                           CTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGT                                                                                       <  SRR19973063.1055319/150‑1 (MQ=60)
                                                               CACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAA                                                                                   >  SRR19973063.3162477/1‑150 (MQ=60)
                                                                     CTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAG                                                                             <  SRR19973063.4882343/150‑1 (MQ=60)
                                                                     CTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAG                                                                             >  SRR19973063.3852111/1‑150 (MQ=60)
                                                                      TTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGG                                                                            <  SRR19973063.504369/150‑1 (MQ=60)
                                                                       TACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGC                                                                           <  SRR19973063.2574937/150‑1 (MQ=60)
                                                                       TACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGC                                                                           <  SRR19973063.2524264/150‑1 (MQ=60)
                                                                          AGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAA                                                                        >  SRR19973063.2672935/1‑150 (MQ=60)
                                                                              TTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTG                                                                    <  SRR19973063.4697149/150‑1 (MQ=60)
                                                                               TCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGA                                                                   <  SRR19973063.585887/150‑1 (MQ=60)
                                                                                   GAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGT                                                               >  SRR19973063.4134142/1‑150 (MQ=60)
                                                                                     AAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGG                                                             >  SRR19973063.2670657/1‑150 (MQ=60)
                                                                                     AAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGG                                                             <  SRR19973063.2547735/150‑1 (MQ=60)
                                                                                     AAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGG                                                             <  SRR19973063.2814682/150‑1 (MQ=60)
                                                                                      AATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGC                                                            >  SRR19973063.3180461/1‑150 (MQ=60)
                                                                                        TGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGAGAGTGGCAT                                                          >  SRR19973063.4409140/1‑150 (MQ=60)
                                                                                         GGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATT                                                         >  SRR19973063.312517/1‑150 (MQ=60)
                                                                                         GGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATT                                                         >  SRR19973063.1712709/1‑150 (MQ=60)
                                                                                          GTCCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTGCAGTGATCTTCCCTCTATGGTTATCGTGCTGTTCGTAATCCCCTAGGTGGTTGTAAGCGTATTTGTTGAACAACTGGCAAATGTGATAGTGGCATTT                                                        <  SRR19973063.2445073/150‑1 (MQ=60)
                                                                                             CAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACC                                                     >  SRR19973063.3867523/1‑150 (MQ=60)
                                                                                              AGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCT                                                    >  SRR19973063.4303967/1‑150 (MQ=60)
                                                                                              AGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCT                                                    >  SRR19973063.556735/1‑150 (MQ=60)
                                                                                              AGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCT                                                    >  SRR19973063.810703/1‑150 (MQ=60)
                                                                                                 CCAGGGTGATCGTCTCTCTCCTGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGAT                                                 <  SRR19973063.253430/150‑1 (MQ=60)
                                                                                                 CCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGAT                                                 <  SRR19973063.636399/150‑1 (MQ=60)
                                                                                                  CAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATT                                                <  SRR19973063.1736004/150‑1 (MQ=60)
                                                                                                   AGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTC                                               >  SRR19973063.995319/1‑150 (MQ=60)
                                                                                                    GGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCA                                              <  SRR19973063.1128159/150‑1 (MQ=60)
                                                                                                      GTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGA                                            >  SRR19973063.3424491/1‑150 (MQ=60)
                                                                                                        GATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAAT                                          <  SRR19973063.4327134/150‑1 (MQ=60)
                                                                                                         ATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATA                                         >  SRR19973063.3591083/1‑150 (MQ=60)
                                                                                                         ATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATA                                         >  SRR19973063.3542620/1‑150 (MQ=60)
                                                                                                              CTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTT                                    <  SRR19973063.5029541/150‑1 (MQ=60)
                                                                                                                CTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTC                                  <  SRR19973063.2250948/150‑1 (MQ=60)
                                                                                                                CTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTC                                  <  SRR19973063.33162/150‑1 (MQ=60)
                                                                                                                CTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTC                                  <  SRR19973063.2250899/150‑1 (MQ=60)
                                                                                                                 TCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCG                                 >  SRR19973063.3101203/1‑150 (MQ=60)
                                                                                                                 TCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCG                                 <  SRR19973063.2006861/150‑1 (MQ=60)
                                                                                                                 TCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCG                                 <  SRR19973063.1552150/150‑1 (MQ=60)
                                                                                                                    CCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCC                              >  SRR19973063.4224590/1‑150 (MQ=60)
                                                                                                                    CCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCC                              <  SRR19973063.1950949/150‑1 (MQ=60)
                                                                                                                       GTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATA                           <  SRR19973063.1648180/150‑1 (MQ=60)
                                                                                                                       GTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATA                           >  SRR19973063.2940088/1‑150 (MQ=60)
                                                                                                                       GTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATA                           <  SRR19973063.4641756/150‑1 (MQ=60)
                                                                                                                        TCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATAT                          <  SRR19973063.2790308/150‑1 (MQ=60)
                                                                                                                        TCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATAT                          <  SRR19973063.4490684/150‑1 (MQ=60)
                                                                                                                         CTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATA                         <  SRR19973063.2434914/150‑1 (MQ=60)
                                                                                                                         CTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATA                         >  SRR19973063.4709319/1‑150 (MQ=60)
                                                                                                                         CTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATA                         >  SRR19973063.3101086/1‑150 (MQ=60)
                                                                                                                         CTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATA                         >  SRR19973063.3101097/1‑150 (MQ=60)
                                                                                                                           CAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAAT                       <  SRR19973063.4795278/150‑1 (MQ=60)
                                                                                                                            AGGGCGCTGTACGAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATA                      <  SRR19973063.4415282/150‑1 (MQ=60)
                                                                                                                            AGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATA                      <  SRR19973063.4414870/150‑1 (MQ=60)
                                                                                                                            AGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGACCATATAATA                      >  SRR19973063.1224307/1‑150 (MQ=60)
                                                                                                                             GGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAA                     >  SRR19973063.2054159/1‑150 (MQ=60)
                                                                                                                              GGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAA                    <  SRR19973063.4530192/150‑1 (MQ=60)
                                                                                                                                   TGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAG               >  SRR19973063.2589667/1‑150 (MQ=60)
                                                                                                                                           GCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCAACGG       >  SRR19973063.3335966/1‑150 (MQ=60)
                                                                                                                                             TCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCAACGGTA     <  SRR19973063.3020218/150‑1 (MQ=60)
                                                                                                                                                AGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCAACGGTAAAT  <  SRR19973063.1234126/150‑1 (MQ=60)
                                                                                                                                                AGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCAACGGTAAAT  <  SRR19973063.1234148/150‑1 (MQ=60)
                                                                                                                                                AGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCAACGGTAAAG  <  SRR19973063.3909016/150‑1 (MQ=60)
                                                                                                                                                |                                                                                                                                                     
GTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCAACGGTAAAT  >  CP009273/288105‑288398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑