Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 317,205 G→A 100% intergenic (+413/‑114) rclR → / → ykgE reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein; putative electron transport chain YkgEFG component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273317,2050GA99.1% 725.6 / ‑2.7 228intergenic (+413/‑114)rclR/ykgEreactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein; putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (116/108);  total (117/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 229 total aligned reads displayed.

TAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGT  >  CP009273/317059‑317351
                                                                                                                                                  |                                                                                                                                                  
taAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAaggg                                                                                                                                                 >  1:3112312/1‑150 (MQ=255)
 aaGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGggt                                                                                                                                                >  1:3009638/1‑150 (MQ=255)
 aaGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGggt                                                                                                                                                <  2:3529942/150‑1 (MQ=255)
  aGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGggtg                                                                                                                                               <  1:1039362/150‑1 (MQ=255)
  aGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGggtg                                                                                                                                               <  1:1328342/150‑1 (MQ=255)
      aTGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTAt                                                                                                                                           >  1:79854/1‑150 (MQ=255)
       tGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATg                                                                                                                                          <  1:3496949/150‑1 (MQ=255)
       tGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATg                                                                                                                                          <  1:19853/150‑1 (MQ=255)
       tGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGACGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGTGTGGTATg                                                                                                                                          >  2:3003137/1‑150 (MQ=255)
        gTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGt                                                                                                                                         <  1:793511/150‑1 (MQ=255)
        gTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGt                                                                                                                                         >  1:2593436/1‑150 (MQ=255)
         tAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGtt                                                                                                                                        <  1:475745/150‑1 (MQ=255)
         tAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGtt                                                                                                                                        >  2:5159033/1‑150 (MQ=255)
            ccATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCt                                                                                                                                     <  2:3455326/150‑1 (MQ=255)
            ccATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCt                                                                                                                                     <  2:4523414/150‑1 (MQ=255)
              aTTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAg                                                                                                                                   <  2:2899240/150‑1 (MQ=255)
                 tttAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCa                                                                                                                                <  2:4212506/150‑1 (MQ=255)
                 tttAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCa                                                                                                                                <  2:1423079/150‑1 (MQ=255)
                   tAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATACAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAAt                                                                                                                              <  1:5066444/150‑1 (MQ=255)
                   tAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAAt                                                                                                                              <  1:1524612/150‑1 (MQ=255)
                    aaaTGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAAtt                                                                                                                             <  1:1052602/150‑1 (MQ=255)
                      aTGGATTTCTTATGCAAAAAATGAACGTCGATTTCCCCATAAAATGTGTGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaa                                                                                                                           <  1:1794812/150‑1 (MQ=255)
                        ggATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaaaa                                                                                                                         >  2:4129091/1‑150 (MQ=255)
                               ttATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACg                                                                                                                  >  1:3334192/1‑150 (MQ=255)
                                 aTGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGcc                                                                                                                <  1:4623360/150‑1 (MQ=255)
                                 aTGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGcc                                                                                                                >  1:4235151/1‑150 (MQ=255)
                                  tGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCa                                                                                                               >  1:251472/1‑150 (MQ=255)
                                   gCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCat                                                                                                              <  1:4680633/150‑1 (MQ=255)
                                    ccAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCata                                                                                                             >  2:857598/1‑150 (MQ=255)
                                    ccAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCata                                                                                                             <  1:4498061/150‑1 (MQ=255)
                                     cAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCatat                                                                                                            >  1:4011316/1‑150 (MQ=255)
                                      aaaaaTGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAtatt                                                                                                           >  2:666436/1‑150 (MQ=255)
                                       aaaaTGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAtattt                                                                                                          >  1:4262722/1‑150 (MQ=255)
                                           tGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtg                                                                                                      >  2:3125707/1‑150 (MQ=255)
                                              tcgtcgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtgatg                                                                                                   >  2:3826748/1‑150 (MQ=255)
                                                gtcgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTgatgat                                                                                                 >  1:4088212/1‑150 (MQ=255)
                                                  cgATTTCCCCATAAACTGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGAtgattg                                                                                               <  1:5040263/150‑1 (MQ=255)
                                                   gATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGAtgattga                                                                                              >  2:423117/1‑150 (MQ=255)
                                                    aTTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGAtgattgat                                                                                             >  2:1559686/1‑150 (MQ=255)
                                                     tttCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATc                                                                                            <  2:4938097/150‑1 (MQ=255)
                                                       tCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATccc                                                                                          >  2:165103/1‑150 (MQ=255)
                                                        ccccATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATcccc                                                                                         <  2:3589683/150‑1 (MQ=255)
                                                         cccATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATccccc                                                                                        >  1:3911269/1‑150 (MQ=255)
                                                         cccATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATccccc                                                                                        <  2:1615662/150‑1 (MQ=255)
                                                          ccATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCg                                                                                       <  1:1557549/150‑1 (MQ=255)
                                                            aTAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGt                                                                                     <  2:2712232/150‑1 (MQ=255)
                                                             tAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTg                                                                                    <  2:3317044/150‑1 (MQ=255)
                                                                aaTGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTgggg                                                                                 >  2:799778/1‑150 (MQ=255)
                                                                aaTGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTgggg                                                                                 >  2:3383942/1‑150 (MQ=255)
                                                                     gAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGaa                                                                            >  2:1241880/1‑150 (MQ=255)
                                                                       gCGATGCCGAAAGAAATAAAATGAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAAc                                                                          <  1:3397400/150‑1 (MQ=255)
                                                                          aTGCCGAAAGAAATAAAATTAGTTATTGCATTTTACGTTTGGATGAAAGATAATCATTTGTCATAAAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAAATCAATATTTATAGATGAATGATCCACCGCAGGGGGGGAAACATc                                                                       >  1:1894290/1‑150 (MQ=255)
                                                                            gCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCgg                                                                     <  1:3158524/150‑1 (MQ=255)
                                                                            gCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCgg                                                                     <  1:4391589/150‑1 (MQ=255)
                                                                               gaaagaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCa                                                                  <  1:2725053/150‑1 (MQ=255)
                                                                               gaaagaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCa                                                                  >  1:93989/1‑150 (MQ=255)
                                                                                aaagaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCAt                                                                 <  1:3342349/150‑1 (MQ=255)
                                                                                  agaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCTGAGGGGGGGAAACATCTGGCATaa                                                               >  1:606200/1‑150 (MQ=255)
                                                                                  agaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATaa                                                               <  2:2568999/150‑1 (MQ=255)
                                                                                  agaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCAGaa                                                               >  2:5092680/1‑150 (MQ=255)
                                                                                  agaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCAGTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATaa                                                               <  2:3934364/150‑1 (MQ=255)
                                                                                    aaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAAt                                                             <  2:709854/150‑1 (MQ=255)
                                                                                      aTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATgg                                                           <  2:1844690/150‑1 (MQ=255)
                                                                                       tAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATggg                                                          <  2:4749921/150‑1 (MQ=255)
                                                                                        aaaaTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGc                                                         >  1:3853865/1‑150 (MQ=255)
                                                                                         aaaTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCa                                                        <  1:3888028/150‑1 (MQ=255)
                                                                                          aaTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCAt                                                       <  1:374540/150‑1 (MQ=255)
                                                                                           aTTAGTTATTGCATTTGATGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATg                                                      >  1:4452828/1‑150 (MQ=255)
                                                                                           aTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATg                                                      <  1:2124189/150‑1 (MQ=255)
                                                                                           aTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATg                                                      <  2:1017022/150‑1 (MQ=255)
                                                                                              aGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAg                                                   <  2:4696099/150‑1 (MQ=255)
                                                                                              aGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAg                                                   >  1:1613290/1‑150 (MQ=255)
                                                                                              aGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCAGACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAg                                                   <  2:5003664/150‑1 (MQ=255)
                                                                                                ttATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCCTAAATGGGCATGAAGTa                                                 >  2:3628164/1‑150 (MQ=255)
                                                                                                 tATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGACACATCGGGCATAAATGGGCATGAAGTaa                                                >  2:1360640/1‑150 (MQ=255)
                                                                                                 tATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa                                                >  1:1039553/1‑150 (MQ=255)
                                                                                                    tGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATgg                                             <  1:786225/150‑1 (MQ=255)
                                                                                                     gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTATCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGa                                            >  1:1286001/1‑150 (MQ=255)
                                                                                                      cATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAg                                           >  2:3975933/1‑150 (MQ=255)
                                                                                                       aTTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGt                                          <  2:3009638/150‑1 (MQ=255)
                                                                                                        tttGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTa                                         >  1:3187372/1‑150 (MQ=255)
                                                                                                        tttGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTa                                         <  2:1505316/150‑1 (MQ=255)
                                                                                                         ttGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAt                                        >  2:2168285/1‑150 (MQ=255)
                                                                                                          tGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAtt                                       <  1:2904658/150‑1 (MQ=255)
                                                                                                          tGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAtt                                       >  2:2427467/1‑150 (MQ=255)
                                                                                                             cGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGt                                    >  1:2820671/1‑150 (MQ=255)
                                                                                                             cGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGt                                    >  1:1424783/1‑150 (MQ=255)
                                                                                                              gTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGTCATGAAGTAATGGAGTATTAGtt                                   >  2:2052485/1‑150 (MQ=255)
                                                                                                               tttGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTtg                                  >  1:4732467/1‑150 (MQ=255)
                                                                                                                 tGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTtgtg                                <  2:3542800/150‑1 (MQ=255)
                                                                                                                 tGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTtgtg                                >  1:1001683/1‑150 (MQ=255)
                                                                                                                     tGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATg                            <  2:411639/150‑1 (MQ=255)
                                                                                                                         aGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGTATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCaa                        <  1:3973473/150‑1 (MQ=255)
                                                                                                                          gATTATCATTTGTCATACAAATAAAGGGTTGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAt                       <  1:628561/150‑1 (MQ=255)
                                                                                                                                cATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt                 <  2:4423470/150‑1 (MQ=255)
                                                                                                                                       cATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCt          >  2:1982007/1‑150 (MQ=255)
                                                                                                                                        aTACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTg         <  1:2859598/150‑1 (MQ=255)
                                                                                                                                        aTACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTg         >  2:2193750/1‑150 (MQ=255)
                                                                                                                                           cAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTAt      >  1:2460862/1‑150 (MQ=255)
                                                                                                                                               tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGt  >  2:4565626/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGT  >  CP009273/317059‑317351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGAC  >  CP009273/317059‑317354
                                                                                                                                                  |                                                                                                                                                     
TAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGG                                                                                                                                                    >  SRR19973063.3122329/1‑150 (MQ=60)
 AAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGT                                                                                                                                                   >  SRR19973063.3019241/1‑150 (MQ=60)
  AGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCCTTTGTCATACAAATAAAGGGTG                                                                                                                                                  <  SRR19973063.1042521/150‑1 (MQ=60)
  AGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTG                                                                                                                                                  <  SRR19973063.1042507/150‑1 (MQ=60)
  AGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTG                                                                                                                                                  <  SRR19973063.1332315/150‑1 (MQ=60)
  AGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTG                                                                                                                                                  >  SRR19973063.4721386/1‑150 (MQ=60)
      ATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTAT                                                                                                                                              >  SRR19973063.80130/1‑150 (MQ=60)
      ATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTAT                                                                                                                                              >  SRR19973063.80121/1‑150 (MQ=60)
       TGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATG                                                                                                                                             <  SRR19973063.19927/150‑1 (MQ=60)
       TGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATG                                                                                                                                             <  SRR19973063.3508131/150‑1 (MQ=60)
        GTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGT                                                                                                                                            >  SRR19973063.2601678/1‑150 (MQ=60)
        GTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGT                                                                                                                                            <  SRR19973063.795803/150‑1 (MQ=60)
         TAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTT                                                                                                                                           <  SRR19973063.477090/150‑1 (MQ=60)
          AGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTG                                                                                                                                          <  SRR19973063.4063906/150‑1 (MQ=60)
                TTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCC                                                                                                                                    <  SRR19973063.3292977/150‑1 (MQ=60)
                 TTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCA                                                                                                                                   <  SRR19973063.309812/150‑1 (MQ=60)
                   TAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATACAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAAT                                                                                                                                 <  SRR19973063.5082749/150‑1 (MQ=60)
                   TAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAAT                                                                                                                                 <  SRR19973063.1529286/150‑1 (MQ=60)
                   TAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAAT                                                                                                                                 <  SRR19973063.1529752/150‑1 (MQ=60)
                    AAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATT                                                                                                                                <  SRR19973063.1055790/150‑1 (MQ=60)
                    AAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATT                                                                                                                                <  SRR19973063.4443827/150‑1 (MQ=60)
                      ATGGATTTCTTATGCAAAAAATGAACGTCGATTTCCCCATAAAATGTGTGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAA                                                                                                                              <  SRR19973063.1800368/150‑1 (MQ=60)
                               TTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACG                                                                                                                     >  SRR19973063.3344823/1‑150 (MQ=60)
                                 ATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCC                                                                                                                   <  SRR19973063.1024481/150‑1 (MQ=60)
                                 ATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCC                                                                                                                   >  SRR19973063.4248651/1‑150 (MQ=60)
                                 ATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCC                                                                                                                   <  SRR19973063.792290/150‑1 (MQ=60)
                                 ATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCC                                                                                                                   <  SRR19973063.4638134/150‑1 (MQ=60)
                                  TGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCA                                                                                                                  >  SRR19973063.252234/1‑150 (MQ=60)
                                  TGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCA                                                                                                                  >  SRR19973063.252226/1‑150 (MQ=60)
                                   GCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAT                                                                                                                 <  SRR19973063.4695556/150‑1 (MQ=60)
                                    CCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATA                                                                                                                <  SRR19973063.1932876/150‑1 (MQ=60)
                                    CCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATA                                                                                                                <  SRR19973063.3346820/150‑1 (MQ=60)
                                    CCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATA                                                                                                                <  SRR19973063.4512500/150‑1 (MQ=60)
                                     CAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATAT                                                                                                               >  SRR19973063.4024181/1‑150 (MQ=60)
                                       AAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTT                                                                                                             >  SRR19973063.1075801/1‑150 (MQ=60)
                                       AAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTT                                                                                                             >  SRR19973063.4276164/1‑150 (MQ=60)
                                       AAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTT                                                                                                             >  SRR19973063.4276284/1‑150 (MQ=60)
                                          ATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATT                                                                                                          <  SRR19973063.1673822/150‑1 (MQ=60)
                                          ATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATT                                                                                                          >  SRR19973063.3928602/1‑150 (MQ=60)
                                           TGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTG                                                                                                         >  SRR19973063.2211312/1‑150 (MQ=60)
                                                GTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGAT                                                                                                    >  SRR19973063.1841783/1‑150 (MQ=60)
                                                GTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGAT                                                                                                    >  SRR19973063.4101313/1‑150 (MQ=60)
                                                  CGATTTCCCCATAAACTGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTG                                                                                                  <  SRR19973063.5056505/150‑1 (MQ=60)
                                                  CGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTG                                                                                                  <  SRR19973063.5057200/150‑1 (MQ=60)
                                                   GATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA                                                                                                 <  SRR19973063.4043366/150‑1 (MQ=60)
                                                     TTTCCACAGAAAATGTGAGCGATGCCGATAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCTTATTTATTGATGATTGATC                                                                                               <  SRR19973063.253656/150‑1 (MQ=60)
                                                         CCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC                                                                                           >  SRR19973063.3923742/1‑150 (MQ=60)
                                                          CCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCG                                                                                          <  SRR19973063.1562321/150‑1 (MQ=60)
                                                            ATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT                                                                                        <  SRR19973063.983357/150‑1 (MQ=60)
                                                               AAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGG                                                                                     <  SRR19973063.614455/150‑1 (MQ=60)
                                                                 ATGTGAGCGATGTCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGTTGGTATGTTGCTAGCCAATTAAAAAAGAACGACATAGATATTGATGATTGATCCCCCGGTGGGGG                                                                                   >  SRR19973063.3896060/1‑150 (MQ=60)
                                                                    TGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGA                                                                                >  SRR19973063.2794928/1‑150 (MQ=60)
                                                                     GAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAA                                                                               <  SRR19973063.2607624/150‑1 (MQ=60)
                                                                       GCGATGCCGAAATAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAACGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACA                                                                            <  SRR19973063.1225532/150‑1 (MQ=60)
                                                                       GCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAAC                                                                             <  SRR19973063.1484843/150‑1 (MQ=60)
                                                                       GCGATGCCGAAAGAAATAAAATGAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAAC                                                                             <  SRR19973063.3408213/150‑1 (MQ=60)
                                                                        CGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGACACA                                                                            >  SRR19973063.2095955/1‑150 (MQ=60)
                                                                          ATGCCGAAAGAAATAAAATTAGTTATTGCATTTTACGTTTGGATGAAAGATAATCATTTGTCATAAAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAAATCAATATTTATAGATGAATGATCCACCGCAGGGGGGGAAACATC                                                                          >  SRR19973063.1900215/1‑150 (MQ=60)
                                                                          ATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATC                                                                          >  SRR19973063.4469843/1‑150 (MQ=60)
                                                                          ATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATC                                                                          >  SRR19973063.412796/1‑150 (MQ=60)
                                                                            GCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGG                                                                        <  SRR19973063.3168692/150‑1 (MQ=60)
                                                                            GCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGG                                                                        <  SRR19973063.4405652/150‑1 (MQ=60)
                                                                            GCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGG                                                                        <  SRR19973063.3168634/150‑1 (MQ=60)
                                                                               GAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCA                                                                     <  SRR19973063.2733849/150‑1 (MQ=60)
                                                                               GAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCA                                                                     >  SRR19973063.94307/1‑150 (MQ=60)
                                                                                AAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCAT                                                                    <  SRR19973063.3353003/150‑1 (MQ=60)
                                                                                AAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCAT                                                                    <  SRR19973063.3353004/150‑1 (MQ=60)
                                                                                 AAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATA                                                                   >  SRR19973063.391576/1‑150 (MQ=60)
                                                                                  AGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCTGAGGGGGGGAAACATCTGGCATAA                                                                  >  SRR19973063.607979/1‑150 (MQ=60)
                                                                                    AAATAATATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCTTTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCTATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAAT                                                                <  SRR19973063.4314152/150‑1 (MQ=60)
                                                                                        AAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGC                                                            <  SRR19973063.1419119/150‑1 (MQ=60)
                                                                                        AAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGC                                                            >  SRR19973063.3866116/1‑150 (MQ=60)
                                                                                         AAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCA                                                           <  SRR19973063.3900376/150‑1 (MQ=60)
                                                                                          AATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCAT                                                          <  SRR19973063.375609/150‑1 (MQ=60)
                                                                                          AATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCAT                                                          <  SRR19973063.2779380/150‑1 (MQ=60)
                                                                                           ATTAGTTATTGCATTTGATGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATG                                                         >  SRR19973063.4467118/1‑150 (MQ=60)
                                                                                           ATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATG                                                         <  SRR19973063.2131059/150‑1 (MQ=60)
                                                                                           ATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATG                                                         <  SRR19973063.2130826/150‑1 (MQ=60)
                                                                                           ATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATG                                                         <  SRR19973063.2895772/150‑1 (MQ=60)
                                                                                           ATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATG                                                         <  SRR19973063.130528/150‑1 (MQ=60)
                                                                                              AGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAG                                                      >  SRR19973063.746538/1‑150 (MQ=60)
                                                                                              AGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAG                                                      >  SRR19973063.1618225/1‑150 (MQ=60)
                                                                                              AGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAG                                                      >  SRR19973063.746288/1‑150 (MQ=60)
                                                                                               GTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGT                                                     <  SRR19973063.3738678/150‑1 (MQ=60)
                                                                                                 TATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAA                                                   >  SRR19973063.1042761/1‑150 (MQ=60)
                                                                                                 TATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAA                                                   >  SRR19973063.1042699/1‑150 (MQ=60)
                                                                                                 TATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAA                                                   <  SRR19973063.3012718/150‑1 (MQ=60)
                                                                                                    TGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTCTGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGG                                                >  SRR19973063.476562/1‑150 (MQ=60)
                                                                                                    TGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGG                                                <  SRR19973063.788502/150‑1 (MQ=60)
                                                                                                     GCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTATCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGA                                               >  SRR19973063.1289827/1‑150 (MQ=60)
                                                                                                       ATTTTACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGT                                             >  SRR19973063.3416120/1‑150 (MQ=60)
                                                                                                       ATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGT                                             >  SRR19973063.3862948/1‑150 (MQ=60)
                                                                                                        TTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTA                                            >  SRR19973063.519357/1‑150 (MQ=60)
                                                                                                        TTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTA                                            >  SRR19973063.3197625/1‑150 (MQ=60)
                                                                                                        TTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTA                                            >  SRR19973063.3197551/1‑150 (MQ=60)
                                                                                                          TGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATT                                          <  SRR19973063.2913928/150‑1 (MQ=60)
                                                                                                          TGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATT                                          <  SRR19973063.1845064/150‑1 (MQ=60)
                                                                                                          TGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATT                                          >  SRR19973063.4287145/1‑150 (MQ=60)
                                                                                                           GACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTA                                         <  SRR19973063.2862257/150‑1 (MQ=60)
                                                                                                             CGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGT                                       >  SRR19973063.2829737/1‑150 (MQ=60)
                                                                                                             CGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGT                                       >  SRR19973063.1429121/1‑150 (MQ=60)
                                                                                                               TTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTG                                     >  SRR19973063.4747546/1‑150 (MQ=60)
                                                                                                               TTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTG                                     >  SRR19973063.5031480/1‑150 (MQ=60)
                                                                                                                TTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGT                                    >  SRR19973063.291351/1‑150 (MQ=60)
                                                                                                                TTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGT                                    <  SRR19973063.83190/150‑1 (MQ=60)
                                                                                                                 TGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTG                                   >  SRR19973063.1004703/1‑150 (MQ=60)
                                                                                                                        AAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCA                            <  SRR19973063.3283688/150‑1 (MQ=60)
                                                                                                                         AGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGTATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAA                           <  SRR19973063.3986197/150‑1 (MQ=60)
                                                                                                                         AGATTATAATTTTTAATACAAATAATGGGTGGTATGTTGCTAGCAAATTAAAAAAGAACGACATATTTATTGATGATTGATCCCCCGGTGGGGGGGAATCATCGGGTATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAA                           <  SRR19973063.3986344/150‑1 (MQ=60)
                                                                                                                          GATTATCATTTGTCATACAAATAAAGGGTTGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAT                          <  SRR19973063.630412/150‑1 (MQ=60)
                                                                                                                          GATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAT                          >  SRR19973063.4670173/1‑150 (MQ=60)
                                                                                                                          GATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAT                          >  SRR19973063.4670154/1‑150 (MQ=60)
                                                                                                                               TCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTT                     >  SRR19973063.3904412/1‑150 (MQ=60)
                                                                                                                                   TTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC                 <  SRR19973063.1568458/150‑1 (MQ=60)
                                                                                                                                    TGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCA                >  SRR19973063.342289/1‑150 (MQ=60)
                                                                                                                                    TGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCA                >  SRR19973063.1466350/1‑150 (MQ=60)
                                                                                                                                    TGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCA                >  SRR19973063.4556437/1‑150 (MQ=60)
                                                                                                                                        ATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTG            <  SRR19973063.2868759/150‑1 (MQ=60)
                                                                                                                                           CAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTAT         >  SRR19973063.2468718/1‑150 (MQ=60)
                                                                                                                                           CAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACAACGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTAT         >  SRR19973063.5022662/1‑150 (MQ=60)
                                                                                                                                            AAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATT        <  SRR19973063.912160/150‑1 (MQ=60)
                                                                                                                                             AATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTG       <  SRR19973063.4048005/150‑1 (MQ=60)
                                                                                                                                                 AAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGA   >  SRR19973063.4622196/1‑150 (MQ=60)
                                                                                                                                                  AGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGAC  >  SRR19973063.278519/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
TAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGAC  >  CP009273/317059‑317354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑