Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 317,205 G→A 100% intergenic (+413/‑114) rclR → / → ykgE reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein; putative electron transport chain YkgEFG component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273317,2050GA99.6% 769.4 / ‑5.9 261intergenic (+413/‑114)rclR/ykgEreactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein; putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (120/134);  minor base T (0/5);  total (120/141)
Fisher's exact test for biased strand distribution p-value = 6.34e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 262 total aligned reads displayed.

ATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTA  >  CP009273/317058‑317346
                                                                                                                                                   |                                                                                                                                             
     ggATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGggtg                                                                                                                                          <  2:271583/148‑1 (MQ=255)
     ggATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGggtg                                                                                                                                          <  2:2716750/148‑1 (MQ=255)
      gATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGggtgg                                                                                                                                         <  1:2688360/148‑1 (MQ=255)
       aTGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGggtggt                                                                                                                                        <  2:2169109/148‑1 (MQ=255)
             ccATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGTATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTg                                                                                                                                  >  1:2497261/1‑148 (MQ=255)
              cATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGc                                                                                                                                 <  1:3019116/148‑1 (MQ=255)
              cATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGc                                                                                                                                 >  2:2307408/1‑148 (MQ=255)
                tttttAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTa                                                                                                                               >  1:859202/1‑148 (MQ=255)
                 ttttAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAg                                                                                                                              >  2:3016075/1‑148 (MQ=255)
                 ttttAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAg                                                                                                                              <  2:615646/148‑1 (MQ=255)
                     aaaTGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCaa                                                                                                                          <  2:42607/148‑1 (MQ=255)
                      aaTGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAAt                                                                                                                         <  1:224579/148‑1 (MQ=255)
                      aaTGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAAt                                                                                                                         <  1:3127299/148‑1 (MQ=255)
                         ggATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaa                                                                                                                      >  2:2848740/1‑148 (MQ=255)
                         ggATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaa                                                                                                                      >  2:2693741/1‑148 (MQ=255)
                          gATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaaa                                                                                                                     >  1:2063730/1‑148 (MQ=255)
                           aTTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATCAAGGGTGGTATGTTGCTAGCCAATTaaaa                                                                                                                    >  2:1982751/1‑148 (MQ=255)
                             ttCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATACAGGGTGGTATGTTGCTAGCCAATTaaaaaa                                                                                                                  >  2:2597435/1‑148 (MQ=255)
                               cTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGa                                                                                                                <  1:732739/148‑1 (MQ=255)
                                    gCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGcc                                                                                                           >  1:920379/1‑148 (MQ=255)
                                       aaaaaTGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCata                                                                                                        >  2:398801/1‑148 (MQ=255)
                                       aaaaaTGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCata                                                                                                        <  1:1483728/148‑1 (MQ=255)
                                        aaaaTGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCatat                                                                                                       <  1:3491543/148‑1 (MQ=255)
                                        aaaaTGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCatat                                                                                                       <  2:1457872/148‑1 (MQ=255)
                                         aaaTGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAtatt                                                                                                      >  1:2468686/1‑148 (MQ=255)
                                           aTGAACGTCGATTTCCCCCTAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAtattta                                                                                                    >  2:1147934/1‑148 (MQ=255)
                                           aTGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAtattta                                                                                                    <  2:2772648/148‑1 (MQ=255)
                                              aacgtcgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtg                                                                                                 <  2:1767173/146‑1 (MQ=255)
                                              aacgtcgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtg                                                                                                 <  1:2848816/146‑1 (MQ=255)
                                               acgtcgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtga                                                                                                >  2:870981/2‑148 (MQ=255)
                                               acgtcgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTAGGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtga                                                                                                >  2:871243/2‑148 (MQ=255)
                                                  tcgATTTCCCCATAAAATGTGAGCGATGCCGAAATAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtgatga                                                                                             <  2:3143863/148‑1 (MQ=255)
                                                   cgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTgatgat                                                                                            <  1:2848358/148‑1 (MQ=255)
                                                   cgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTgatgat                                                                                            <  1:401710/148‑1 (MQ=255)
                                                         ccccATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATcc                                                                                      >  2:1136886/1‑148 (MQ=255)
                                                              tAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCgg                                                                                 <  2:1335110/148‑1 (MQ=255)
                                                               aaaaTGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGt                                                                                >  1:1220369/1‑148 (MQ=255)
                                                                aaaTGTGAGCGATGCCTAAATAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTg                                                                               <  1:2415515/148‑1 (MQ=255)
                                                                aaaTGTGAGCGATGCCGAAAGAAATAAAATTAGTTTGTGCATTTGACGTTTGGATGAAATATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCATTTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTg                                                                               <  2:1589024/148‑1 (MQ=255)
                                                                        gCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGaa                                                                       <  2:777882/148‑1 (MQ=255)
                                                                          gATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAACGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAAc                                                                     <  1:39260/148‑1 (MQ=255)
                                                                           aTGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACa                                                                    >  1:1981884/1‑148 (MQ=255)
                                                                           aTGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACa                                                                    <  2:2063730/148‑1 (MQ=255)
                                                                              ccGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCg                                                                 <  1:1621293/148‑1 (MQ=255)
                                                                                gaaagaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCggg                                                               <  2:2883382/148‑1 (MQ=255)
                                                                                gaaagaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCggg                                                               >  1:738002/1‑148 (MQ=255)
                                                                                   agaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCAt                                                            <  2:1438114/148‑1 (MQ=255)
                                                                                     aaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATaa                                                          >  1:2924912/1‑148 (MQ=255)
                                                                                      aaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATaaa                                                         <  2:210635/148‑1 (MQ=255)
                                                                                       aTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAAt                                                        <  1:1097827/148‑1 (MQ=255)
                                                                                        tAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATg                                                       <  2:920379/148‑1 (MQ=255)
                                                                                        tAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATg                                                       <  1:665469/148‑1 (MQ=255)
                                                                                           aaTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGc                                                    <  1:522062/148‑1 (MQ=255)
                                                                                              tAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATg                                                 >  1:94706/1‑148 (MQ=255)
                                                                                              tAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATg                                                 <  2:705437/148‑1 (MQ=255)
                                                                                              tAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATg                                                 >  2:3477642/1‑148 (MQ=255)
                                                                                              tAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATg                                                 >  1:2782767/1‑148 (MQ=255)
                                                                                                 ttATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAg                                              <  1:1138022/148‑1 (MQ=255)
                                                                                                 ttATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAg                                              >  1:1146379/1‑148 (MQ=255)
                                                                                                 ttATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGGGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAg                                              <  2:2751179/148‑1 (MQ=255)
                                                                                                  tATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGt                                             >  1:1713319/1‑148 (MQ=255)
                                                                                                  tATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGt                                             >  1:1552789/1‑148 (MQ=255)
                                                                                                   aTTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTa                                            <  1:3363125/148‑1 (MQ=255)
                                                                                                    ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa                                           <  2:2924936/148‑1 (MQ=255)
                                                                                                     tGCATTTGACGTTTGTATGAAAGATTATCATTTGTCATACAAATAAAGGGTTGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTTATGATTGATCACCAGGTGTGGGGGAAACATAGGGCATAAATGGGCATGAAGTAAt                                          >  1:1375502/1‑148 (MQ=255)
                                                                                                     tGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt                                          <  1:1800451/148‑1 (MQ=255)
                                                                                                     tGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt                                          <  1:2994222/148‑1 (MQ=255)
                                                                                                        aTTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGa                                       <  2:3338023/148‑1 (MQ=255)
                                                                                                         tttGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAg                                      >  1:1293952/1‑148 (MQ=255)
                                                                                                            gACGTTTGGATGAAAGATTATCTTTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAt                                   <  2:1832303/148‑1 (MQ=255)
                                                                                                            gACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATgg                                                      <  1:852174/129‑1 (MQ=255)
                                                                                                            gACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATgg                                                      >  2:852174/1‑129 (MQ=255)
                                                                                                            gACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAt                                   <  1:1037417/148‑1 (MQ=255)
                                                                                                             aCGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGGTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAtt                                  >  1:1804380/1‑148 (MQ=255)
                                                                                                             aCGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAtt                                  >  1:2397131/1‑148 (MQ=255)
                                                                                                              cGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTa                                 >  2:2411436/1‑148 (MQ=255)
                                                                                                               gTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAg                                >  1:3155593/1‑148 (MQ=255)
                                                                                                                 ttGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGtt                              >  2:103527/1‑148 (MQ=255)
                                                                                                                 ttGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGtt                              >  1:1774374/1‑148 (MQ=255)
                                                                                                                  tttaTGAAAGTTTATCATTTGTAATACAAATAAAGGGTGGTATGTTGCTAGACATTTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTtg                             <  2:1689030/145‑1 (MQ=255)
                                                                                                                  tGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTtg                             <  1:585903/148‑1 (MQ=255)
                                                                                                                  tGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTtg                             <  2:1689974/148‑1 (MQ=255)
                                                                                                                   ggATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTCTTAGTtgt                            >  2:2906786/1‑148 (MQ=255)
                                                                                                                    gATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTtgtg                           >  1:823088/1‑148 (MQ=255)
                                                                                                                     aTGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGa                          >  2:3452879/1‑148 (MQ=255)
                                                                                                                          aGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATgg                                        >  1:2567756/1‑129 (MQ=255)
                                                                                                                              tATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAtt                 >  1:2788026/1‑148 (MQ=255)
                                                                                                                              tATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAtt                 >  1:1251521/1‑148 (MQ=255)
                                                                                                                               aTCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAttt                <  2:1602884/148‑1 (MQ=255)
                                                                                                                               aTCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGGGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAttt                >  2:2389400/1‑148 (MQ=255)
                                                                                                                                tCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTc               <  2:2524271/148‑1 (MQ=255)
                                                                                                                                 cATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCt              <  2:2244685/148‑1 (MQ=255)
                                                                                                                                 cATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCt              >  2:1626357/1‑148 (MQ=255)
                                                                                                                                        cATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc       <  2:1424553/148‑1 (MQ=255)
                                                                                                                                        cATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc       <  1:2566955/148‑1 (MQ=255)
                                                                                                                                        aaTACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc       <  2:1821253/147‑1 (MQ=255)
                                                                                                                                          tACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCt     <  2:3012005/148‑1 (MQ=255)
                                                                                                                                           aCAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTg    >  2:1283286/1‑148 (MQ=255)
                                                                                                                                           aCAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTg    <  2:2864328/148‑1 (MQ=255)
                                                                                                                                             aaaTAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTa  <  1:2162906/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
ATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTA  >  CP009273/317058‑317346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGAC  >  CP009273/317056‑317354
                                                                                                                                                     |                                                                                                                                                     
GTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAG                                                                                                                                                       >  SRR19973065.379267/1‑150 (MQ=60)
GTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAA                                                                                                                                                       <  SRR19973065.128501/150‑1 (MQ=60)
      GGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGG                                                                                                                                                 <  SRR19973065.430965/150‑1 (MQ=60)
        GATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTA                                                                                                                                               <  SRR19973065.2705049/150‑1 (MQ=60)
           GTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTAggg                                                                                                                                            >  SRR19973065.943573/1‑147 (MQ=60)
           GTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGT                                                                                                                                            >  SRR19973065.2875479/1‑150 (MQ=60)
            TAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTT                                                                                                                                           >  SRR19973065.1070701/1‑150 (MQ=60)
            TAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTT                                                                                                                                           <  SRR19973065.85791/150‑1 (MQ=60)
            TAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTT                                                                                                                                           <  SRR19973065.2820608/150‑1 (MQ=60)
            TAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTT                                                                                                                                           <  SRR19973065.2588958/150‑1 (MQ=60)
            TAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTT                                                                                                                                           <  SRR19973065.2588699/150‑1 (MQ=60)
             AGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGTATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTG                                                                                                                                          >  SRR19973065.2512973/1‑150 (MQ=60)
               CCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCT                                                                                                                                        <  SRR19973065.2433447/150‑1 (MQ=60)
                CATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTA                                                                                                                                       >  SRR19973065.864741/1‑150 (MQ=60)
                CATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTA                                                                                                                                       <  SRR19973065.3037612/150‑1 (MQ=60)
                CATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTA                                                                                                                                       <  SRR19973065.3037391/150‑1 (MQ=60)
                 ATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAG                                                                                                                                      >  SRR19973065.2949052/1‑150 (MQ=60)
                     TTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAAATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAA                                                                                                                                  <  SRR19973065.892458/150‑1 (MQ=60)
                       AAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATT                                                                                                                                >  SRR19973065.1336226/1‑150 (MQ=60)
                        AATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTA                                                                                                                               <  SRR19973065.225261/150‑1 (MQ=60)
                        AATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTA                                                                                                                               <  SRR19973065.226237/150‑1 (MQ=60)
                        AATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTA                                                                                                                               <  SRR19973065.3146337/150‑1 (MQ=60)
                          TGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAA                                                                                                                             >  SRR19973065.637671/1‑150 (MQ=60)
                          TGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAA                                                                                                                             >  SRR19973065.2076889/1‑150 (MQ=60)
                          TGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAA                                                                                                                             >  SRR19973065.1764524/1‑150 (MQ=60)
                          TGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAA                                                                                                                             >  SRR19973065.1764083/1‑150 (MQ=60)
                            GATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAA                                                                                                                           <  SRR19973065.449633/150‑1 (MQ=60)
                            GATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAA                                                                                                                           >  SRR19973065.3290224/1‑150 (MQ=60)
                              TTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAG                                                                                                                         <  SRR19973065.1124792/150‑1 (MQ=60)
                                 CTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAAC                                                                                                                      <  SRR19973065.737642/150‑1 (MQ=60)
                                 CTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAAC                                                                                                                      >  SRR19973065.2091068/1‑150 (MQ=60)
                                 CTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAAC                                                                                                                      <  SRR19973065.738515/150‑1 (MQ=60)
                                    ATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGGTTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCC                                                                                                                   >  SRR19973065.716737/1‑150 (MQ=60)
                                    ATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCC                                                                                                                   >  SRR19973065.710135/1‑150 (MQ=60)
                                    ATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCC                                                                                                                   >  SRR19973065.926410/1‑150 (MQ=60)
                                    ATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCC                                                                                                                   >  SRR19973065.926123/1‑150 (MQ=60)
                                       CAAAAAATGAACTTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATTAAAGATTATCATTTGTCATAAAAATAAAGGGTGGTATGTTGCTAGCAAATTAAAAAAGAACGCCATA                                                                                                                <  SRR19973065.2709392/150‑1 (MQ=60)
                                         AAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATT                                                                                                              >  SRR19973065.2484236/1‑150 (MQ=60)
                                         AAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATT                                                                                                              >  SRR19973065.2337315/1‑150 (MQ=60)
                                         AAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATT                                                                                                              <  SRR19973065.1493192/150‑1 (MQ=60)
                                          AAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTT                                                                                                             <  SRR19973065.3512769/150‑1 (MQ=60)
                                          AAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTT                                                                                                             <  SRR19973065.3512775/150‑1 (MQ=60)
                                            AATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAATAAAGCCATATATAT                                                                                                           >  SRR19973065.2258938/1‑150 (MQ=60)
                                            AATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTAT                                                                                                           >  SRR19973065.24708/1‑150 (MQ=60)
                                            AATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTAT                                                                                                           >  SRR19973065.316150/1‑150 (MQ=60)
                                            AATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTAT                                                                                                           >  SRR19973065.1816037/1‑150 (MQ=60)
                                              ttaacgtctATTTCCCCATAAAATGTGAGCTATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCTTTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCATTTAAAAAAGAACGCCATATTTATTG                                                                                                         <  SRR19973065.1292572/141‑1 (MQ=60)
                                               caaCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAACAACGCCATATTTATTGA                                                                                                        <  SRR19973065.2525655/147‑1 (MQ=60)
                                                ACCGTCGATTTCCCCATAAAATGTGAGCGATGCCTAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGAT                                                                                                       <  SRR19973065.2866332/150‑1 (MQ=60)
                                                AACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGAT                                                                                                       <  SRR19973065.2866341/150‑1 (MQ=60)
                                                   GTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGAT                                                                                                    <  SRR19973065.1914637/150‑1 (MQ=60)
                                                    TCGATGTCCCCATAAAATTTGAGCGATGCCGAAACAAATAAATTTATTTATTGCATTTGACTTTTGGATGAAATATTATCATTTGTCATACAAATAATGGTTGGTATGTTGCTAGCCAATTAAAAAACAACGCCATATTTATTGATGATT                                                                                                   <  SRR19973065.2744703/150‑1 (MQ=60)
                                                     GGATTTCCCCATAAAATGTGAGCTATGCCGAAAGAAATAAAATTAGTTTTTGCATTTGACGTTTGGATGAAATATTATCATTTTTCATAAAAATAATGGGTGGTATGTTGCTAGCAAATTAAAAAAGAACGCCATATTTATTGATGATTG                                                                                                  <  SRR19973065.404525/150‑1 (MQ=60)
                                                     CGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTG                                                                                                  <  SRR19973065.404527/150‑1 (MQ=60)
                                                     CGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTG                                                                                                  <  SRR19973065.2865881/150‑1 (MQ=60)
                                                     CGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTG                                                                                                  >  SRR19973065.989731/1‑150 (MQ=60)
                                                        TTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTTTTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAATTACAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC                                                                                               <  SRR19973065.2495243/150‑1 (MQ=60)
                                                           CCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC                                                                                            <  SRR19973065.1305132/150‑1 (MQ=60)
                                                            CCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC                                                                                           >  SRR19973065.2900124/1‑150 (MQ=60)
                                                               ATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT                                                                                        >  SRR19973065.1228302/1‑150 (MQ=60)
                                                               ATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT                                                                                        >  SRR19973065.1228301/1‑150 (MQ=60)
                                                               ATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT                                                                                        >  SRR19973065.1226608/1‑150 (MQ=60)
                                                               ATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATACCCCGGG                                                                                        >  SRR19973065.1230359/1‑150 (MQ=60)
                                                                  AAATGTGAGCGATGCCTAAATAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGG                                                                                     <  SRR19973065.2430731/150‑1 (MQ=60)
                                                                  AAATGTGAGCGATGCCGAAAGAAATAAAATTCGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGG                                                                                     <  SRR19973065.2430009/150‑1 (MQ=60)
                                                                  AAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAATGGGTGTTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGG                                                                                     <  SRR19973065.1553162/150‑1 (MQ=60)
                                                                           CGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACA                                                                            >  SRR19973065.1995167/1‑150 (MQ=60)
                                                                           CGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACA                                                                            >  SRR19973065.1994767/1‑150 (MQ=60)
                                                                            GATGCCTAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAACGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACAT                                                                           <  SRR19973065.39737/150‑1 (MQ=60)
                                                                            GAGGCCTAAATAAATAAAATTAGTTATTGCATTTGACGTTTGTATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAACAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACAT                                                                           <  SRR19973065.41100/150‑1 (MQ=60)
                                                                             ATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATC                                                                          <  SRR19973065.1790237/150‑1 (MQ=60)
                                                                                CCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGG                                                                       <  SRR19973065.1632158/150‑1 (MQ=60)
                                                                                CCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGG                                                                       >  SRR19973065.742937/1‑150 (MQ=60)
                                                                                CCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGG                                                                       >  SRR19973065.60846/1‑150 (MQ=60)
                                                                                  GAATGAAATAAAATTAGTTAGTTAATTTGACGTTTTGTTGAAAGATTTTCATTTGTCTTACAAATAACGGGTGGTATGTTGCTAGCAAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCA                                                                     <  SRR19973065.3150876/150‑1 (MQ=60)
                                                                                  GAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCA                                                                     >  SRR19973065.3050964/1‑150 (MQ=60)
                                                                                  GAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCA                                                                     <  SRR19973065.1393603/150‑1 (MQ=60)
                                                                                     AGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAA                                                                  >  SRR19973065.2942680/1‑150 (MQ=60)
                                                                                     AGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAA                                                                  >  SRR19973065.2942704/1‑150 (MQ=60)
                                                                                         ATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGG                                                              <  SRR19973065.1105049/150‑1 (MQ=60)
                                                                                         ATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGG                                                              >  SRR19973065.2518394/1‑150 (MQ=60)
                                                                                          TAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGG                                                             <  SRR19973065.3164498/150‑1 (MQ=60)
                                                                                          TAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGG                                                             <  SRR19973065.669892/150‑1 (MQ=60)
                                                                                           gtgtgctcttccgatcTTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGC                                                            <  SRR19973065.857687/134‑1 (MQ=60)
                                                                                            AAATTAGTTATTGATTTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAATGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCA                                                           <  SRR19973065.1554794/150‑1 (MQ=60)
                                                                                             AATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCAT                                                          <  SRR19973065.525500/150‑1 (MQ=60)
                                                                                             AATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCAT                                                          <  SRR19973065.1708280/150‑1 (MQ=60)
                                                                                              ATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATG                                                         >  SRR19973065.2800373/1‑150 (MQ=60)
                                                                                              ATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATG                                                         >  SRR19973065.95594/1‑150 (MQ=60)
                                                                                              ATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATG                                                         >  SRR19973065.2800032/1‑150 (MQ=60)
                                                                                                TAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAA                                                       >  SRR19973065.826635/1‑150 (MQ=60)
                                                                                                 AGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAG                                                      >  SRR19973065.1153776/1‑150 (MQ=60)
                                                                                                  GTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGT                                                     >  SRR19973065.1724713/1‑150 (MQ=60)
                                                                                                  GTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGT                                                     >  SRR19973065.1563085/1‑150 (MQ=60)
                                                                                                  GTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGT                                                     >  SRR19973065.1563249/1‑150 (MQ=60)
                                                                                                   TTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTA                                                    <  SRR19973065.1144913/150‑1 (MQ=60)
                                                                                                   TTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTA                                                    <  SRR19973065.858588/150‑1 (MQ=60)
                                                                                                   TTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTA                                                    <  SRR19973065.858619/150‑1 (MQ=60)
                                                                                                   TTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTA                                                    <  SRR19973065.1145392/150‑1 (MQ=60)
                                                                                                     ATTGCATTTGACGTTTGTATGAAAGATTATCATTTGTCATACAAATAAAGGGTTGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTTATGATTGATCACCAGGTGTGGGGGAAACATAGGGCATAAATGGGCATGAAGTAAT                                                  >  SRR19973065.1384577/1‑150 (MQ=60)
                                                                                                     ATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAT                                                  <  SRR19973065.3383687/150‑1 (MQ=60)
                                                                                                       TGCATTTGACGTTTGTATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGG                                                <  SRR19973065.1812275/150‑1 (MQ=60)
                                                                                                       TGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGG                                                <  SRR19973065.1812276/150‑1 (MQ=60)
                                                                                                       TGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGG                                                <  SRR19973065.3012558/150‑1 (MQ=60)
                                                                                                         CATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAG                                              >  SRR19973065.1302479/1‑150 (MQ=60)
                                                                                                             TGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGGTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATT                                          >  SRR19973065.1816245/1‑150 (MQ=60)
                                                                                                             TGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATT                                          >  SRR19973065.2412207/1‑150 (MQ=60)
                                                                                                             TGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATT                                          >  SRR19973065.2412204/1‑150 (MQ=60)
                                                                                                              GACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTA                                         <  SRR19973065.1044285/150‑1 (MQ=60)
                                                                                                               ACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAG                                        >  SRR19973065.3174731/1‑150 (MQ=60)
                                                                                                               ACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAG                                        <  SRR19973065.2136370/150‑1 (MQ=60)
                                                                                                                 GTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTT                                      >  SRR19973065.1785982/1‑150 (MQ=60)
                                                                                                                 GTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTT                                      <  SRR19973065.1152968/150‑1 (MQ=60)
                                                                                                                    TGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTG                                   >  SRR19973065.828490/1‑150 (MQ=60)
                                                                                                                    TGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTG                                   <  SRR19973065.589549/150‑1 (MQ=60)
                                                                                                                          AAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGagatcggaagagcacac                             >  SRR19973065.2583789/1‑133 (MQ=60)
                                                                                                                              ATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATT                         >  SRR19973065.2805703/1‑150 (MQ=60)
                                                                                                                              ATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATT                         >  SRR19973065.2805312/1‑150 (MQ=60)
                                                                                                                              ATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATT                         >  SRR19973065.1984988/1‑150 (MQ=60)
                                                                                                                              ATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATT                         >  SRR19973065.1259760/1‑150 (MQ=60)
                                                                                                                                    ATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTG                   <  SRR19973065.3091598/150‑1 (MQ=60)
                                                                                                                                       TGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCA                >  SRR19973065.3419773/1‑150 (MQ=60)
                                                                                                                                          CATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCT             <  SRR19973065.2583035/150‑1 (MQ=60)
                                                                                                                                          CATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCT             <  SRR19973065.2582985/150‑1 (MQ=60)
                                                                                                                                           ATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTG            >  SRR19973065.1241068/1‑150 (MQ=60)
                                                                                                                                             ACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTA          >  SRR19973065.578347/1‑150 (MQ=60)
                                                                                                                                             ACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTA          >  SRR19973065.746492/1‑150 (MQ=60)
                                                                                                                                               AAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATT        >  SRR19973065.458679/1‑150 (MQ=60)
                                                                                                                                               AAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATT        <  SRR19973065.398350/150‑1 (MQ=60)
                                                                                                                                               AAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATT        <  SRR19973065.2176808/150‑1 (MQ=60)
                                                                                                                                               AAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATT        <  SRR19973065.1623836/150‑1 (MQ=60)
                                                                                                                                                AATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTG       <  SRR19973065.334469/150‑1 (MQ=60)
                                                                                                                                                     AGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGAC  >  SRR19973065.369971/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGAC  >  CP009273/317056‑317354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑