Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 667,348 G→T 100% Q99K (CAG→AAG)  lptE ← LPS assembly OM complex LptDE, lipoprotein component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273667,3480GT96.3% 862.2 / 16.0 282Q99K (CAG→AAG) lptELPS assembly OM complex LptDE, lipoprotein component
Reads supporting (aligned to +/- strand):  ref base G (9/2);  new base T (129/142);  total (138/144)
Fisher's exact test for biased strand distribution p-value = 3.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 283 total aligned reads displayed.

ACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCG  >  CP009273/667201‑667494
                                                                                                                                                   |                                                                                                                                                  
     tctTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATActct                                                                                                                                             <  1:2448533/150‑1 (MQ=255)
     tctTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATActct                                                                                                                                             <  2:1625829/150‑1 (MQ=255)
     tctTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATActct                                                                                                                                             <  2:2849811/150‑1 (MQ=255)
        tttACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTctgct                                                                                                                                          >  2:2558641/1‑150 (MQ=255)
            cGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGttt                                                                                                                                      <  1:4364219/150‑1 (MQ=255)
             gATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTg                                                                                                                                     >  2:2126261/1‑150 (MQ=255)
             gATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTg                                                                                                                                     <  1:2211586/150‑1 (MQ=255)
              aTCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGa                                                                                                                                    >  2:1316822/1‑150 (MQ=255)
               tCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGAc                                                                                                                                   >  2:2909184/1‑150 (MQ=255)
                cATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGAcc                                                                                                                                  <  2:3716922/150‑1 (MQ=255)
                cATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGAcc                                                                                                                                  <  1:614234/150‑1 (MQ=255)
                 aTGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCg                                                                                                                                 <  2:1034893/150‑1 (MQ=255)
                    tCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTa                                                                                                                              <  1:2558641/150‑1 (MQ=255)
                    tCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTa                                                                                                                              <  1:404506/150‑1 (MQ=255)
                    tCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTa                                                                                                                              <  2:3065718/150‑1 (MQ=255)
                    tCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTa                                                                                                                              >  2:3183005/1‑150 (MQ=255)
                       tGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACgg                                                                                                                           >  1:1008460/1‑150 (MQ=255)
                        gTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGa                                                                                                                          <  2:4230285/150‑1 (MQ=255)
                           cGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATa                                                                                                                       <  1:1039488/150‑1 (MQ=255)
                            gTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATAc                                                                                                                      >  2:277623/1‑150 (MQ=255)
                                   ttCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCg                                                                                                               >  1:281250/1‑150 (MQ=255)
                                   ttCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCg                                                                                                               <  2:115910/150‑1 (MQ=255)
                                   ttCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCg                                                                                                               <  1:3284753/150‑1 (MQ=255)
                                     cGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGt                                                                                                             >  1:3699093/1‑150 (MQ=255)
                                     cGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGt                                                                                                             <  1:1894288/150‑1 (MQ=255)
                                        tAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATc                                                                                                          >  1:302679/1‑150 (MQ=255)
                                        tAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATc                                                                                                          >  1:210422/1‑150 (MQ=255)
                                         aaCGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCt                                                                                                         <  2:3968668/150‑1 (MQ=255)
                                           cGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCttt                                                                                                       >  2:4392102/1‑150 (MQ=255)
                                            gCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTc                                                                                                      >  1:396922/1‑150 (MQ=255)
                                            gCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTc                                                                                                      >  1:2021616/1‑150 (MQ=255)
                                             ccATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTcg                                                                                                     >  2:4780499/1‑150 (MQ=255)
                                              cATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTcgc                                                                                                    >  2:1271131/1‑150 (MQ=255)
                                                 ttGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGAt                                                                                                 <  2:2263117/150‑1 (MQ=255)
                                                 ttGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGAt                                                                                                 <  2:4983015/150‑1 (MQ=255)
                                                 ttGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGAt                                                                                                 >  1:1814220/1‑150 (MQ=255)
                                                  tGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATg                                                                                                <  1:1691207/150‑1 (MQ=255)
                                                  tGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATg                                                                                                >  1:521613/1‑150 (MQ=255)
                                                         tATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACtt                                                                                         <  2:4083336/150‑1 (MQ=255)
                                                         tATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACtt                                                                                         >  1:3356180/1‑150 (MQ=255)
                                                             gaagaaCGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTAcc                                                                                     <  2:3377778/150‑1 (MQ=255)
                                                              aagaaCGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGGTTGACCGGTACGGAATACCGATGCGGGATCTTTCGCGATGCTCACTTGAccc                                                                                    >  2:5011806/1‑150 (MQ=255)
                                                                gaacgaacGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCaa                                                                                  <  2:3928788/150‑1 (MQ=255)
                                                                  acgaacgGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAc                                                                                <  1:3196428/150‑1 (MQ=255)
                                                                   cgaacgGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACg                                                                               >  1:512487/1‑150 (MQ=255)
                                                                   cgaacgGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACg                                                                               <  2:1767660/150‑1 (MQ=255)
                                                                    gaacgGAAGACTTTGGCGCTACTCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTAGCTTTCGCGATGCTCACTTTACCCAAACGc                                                                              >  2:3665875/1‑150 (MQ=255)
                                                                    gaacgGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGc                                                                              >  2:1081180/1‑150 (MQ=255)
                                                                    gaacgGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGc                                                                              >  1:1416471/1‑150 (MQ=255)
                                                                          aaGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGAt                                                                        >  2:191930/1‑150 (MQ=255)
                                                                          aaGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGAt                                                                        <  2:1995753/150‑1 (MQ=255)
                                                                           aGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATg                                                                       <  1:2647880/150‑1 (MQ=255)
                                                                            gACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATgg                                                                      >  2:1142202/1‑150 (MQ=255)
                                                                              cTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGaa                                                                    <  1:2416367/150‑1 (MQ=255)
                                                                              cTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGaa                                                                    <  2:2668772/150‑1 (MQ=255)
                                                                               tttGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAAc                                                                   <  1:3086070/150‑1 (MQ=255)
                                                                               tttGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAAc                                                                   <  1:417120/150‑1 (MQ=255)
                                                                                ttGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACg                                                                  <  1:1190522/150‑1 (MQ=255)
                                                                                ttGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACg                                                                  <  2:4640768/150‑1 (MQ=255)
                                                                                  ggCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTc                                                                >  1:2059047/1‑150 (MQ=255)
                                                                                    cgcTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCt                                                              >  2:1246573/1‑150 (MQ=255)
                                                                                     gcTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCtt                                                             <  1:894700/150‑1 (MQ=255)
                                                                                       tAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTGTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTAc                                                           >  1:1872140/1‑150 (MQ=255)
                                                                                         aTCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTAcgc                                                         <  1:4404641/150‑1 (MQ=255)
                                                                                            gggTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTg                                                      >  2:1480798/1‑150 (MQ=255)
                                                                                             ggTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTgg                                                     >  1:5071213/1‑150 (MQ=255)
                                                                                              gTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGt                                                    <  2:512617/150‑1 (MQ=255)
                                                                                                aGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGttt                                                  <  1:4495300/150‑1 (MQ=255)
                                                                                                aGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGttt                                                  <  1:1622282/150‑1 (MQ=255)
                                                                                                 gATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTc                                                 <  1:749273/150‑1 (MQ=255)
                                                                                                      cACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTAt                                            <  1:4656854/150‑1 (MQ=255)
                                                                                                       aCGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATc                                           <  1:3006084/150‑1 (MQ=255)
                                                                                                        cGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCa                                          <  1:1704436/150‑1 (MQ=255)
                                                                                                         ggCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCaa                                         >  2:840709/1‑150 (MQ=255)
                                                                                                           ccGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGc                                       >  1:3661798/1‑150 (MQ=255)
                                                                                                              gggATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAAc                                    >  2:691202/1‑150 (MQ=255)
                                                                                                               ggATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACt                                   <  2:1788651/150‑1 (MQ=255)
                                                                                                               ggATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACt                                   >  2:2232836/1‑150 (MQ=255)
                                                                                                               ggATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACt                                   <  2:2285322/150‑1 (MQ=255)
                                                                                                               ggATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACt                                   <  2:3449119/150‑1 (MQ=255)
                                                                                                                 aTCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCg                                 <  2:4542147/150‑1 (MQ=255)
                                                                                                                  tCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGa                                <  1:3463037/150‑1 (MQ=255)
                                                                                                                    aaCACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGaca                              >  2:3537990/1‑150 (MQ=255)
                                                                                                                    aaCACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGaca                              >  2:3538206/1‑150 (MQ=255)
                                                                                                                      cacGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACAcc                            <  2:1416471/150‑1 (MQ=255)
                                                                                                                        cGGTCGCTTTAACAGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACTGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCTAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACAACAt                          <  2:4868603/150‑1 (MQ=255)
                                                                                                                         ggTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCAtt                         <  1:2290701/150‑1 (MQ=255)
                                                                                                                          gTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCGTACGCGTGGTTTCTTTATCAAGCACCTCGACACCATTc                        >  1:2714967/1‑150 (MQ=255)
                                                                                                                            cGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAg                      <  2:3225544/150‑1 (MQ=255)
                                                                                                                              cATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGAc                    >  1:2894202/1‑150 (MQ=255)
                                                                                                                               aTTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACg                   <  1:85597/150‑1 (MQ=255)
                                                                                                                                 tAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTa                 >  1:3937582/1‑150 (MQ=255)
                                                                                                                                  aaCCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTaa                <  2:4412488/150‑1 (MQ=255)
                                                                                                                                  aaCCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTaa                <  2:5025651/150‑1 (MQ=255)
                                                                                                                                  aaCCGTCATGATCATCTTATACTCTGCTGTGTGACCGTTACGGAATACCGATGCTGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTaa                <  2:4412713/150‑1 (MQ=255)
                                                                                                                                   aCCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAAc               <  1:4226061/150‑1 (MQ=255)
                                                                                                                                    ccGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACt              <  2:4168274/150‑1 (MQ=255)
                                                                                                                                    ccGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACt              >  2:430294/1‑150 (MQ=255)
                                                                                                                                            atcatcTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAcgc      >  1:4723194/1‑150 (MQ=255)
                                                                                                                                             tcatcTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTGACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAcgca     <  2:3815704/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCG  >  CP009273/667201‑667494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCG  >  CP009273/667199‑667494
                                                                                                                                                     |                                                                                                                                                  
GTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTT                                                                                                                                                    <  SRR19973063.2179064/150‑1 (MQ=60)
GTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTT                                                                                                                                                    <  SRR19973063.3636337/150‑1 (MQ=60)
GTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTA                                                                                                                                                    <  SRR19973063.2179050/150‑1 (MQ=60)
 TACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTA                                                                                                                                                   >  SRR19973063.5102534/1‑150 (MQ=60)
  ACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCTAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTAT                                                                                                                                                  <  SRR19973063.3524336/150‑1 (MQ=60)
  ACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTAT                                                                                                                                                  <  SRR19973063.4970955/150‑1 (MQ=60)
   CATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATA                                                                                                                                                 >  SRR19973063.1649611/1‑150 (MQ=60)
    ATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATAC                                                                                                                                                <  SRR19973063.337632/150‑1 (MQ=60)
      CTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTC                                                                                                                                              >  SRR19973063.695938/1‑150 (MQ=60)
       TCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCT                                                                                                                                             <  SRR19973063.2456349/150‑1 (MQ=60)
          TTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCT                                                                                                                                          <  SRR19973063.3248424/150‑1 (MQ=60)
             ACGATCATGTCTTGTTCGTCATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTT                                                                                                                                       <  SRR19973063.5125106/150‑1 (MQ=60)
              CGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTT                                                                                                                                      <  SRR19973063.4378181/150‑1 (MQ=60)
              CGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTT                                                                                                                                      <  SRR19973063.3682824/150‑1 (MQ=60)
               GATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTG                                                                                                                                     <  SRR19973063.2218555/150‑1 (MQ=60)
                  CATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACC                                                                                                                                  <  SRR19973063.616036/150‑1 (MQ=60)
                   ATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCG                                                                                                                                 <  SRR19973063.2651495/150‑1 (MQ=60)
                    TGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGT                                                                                                                                >  SRR19973063.3638734/1‑150 (MQ=60)
                      TCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTA                                                                                                                              <  SRR19973063.405644/150‑1 (MQ=60)
                      TCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTA                                                                                                                              <  SRR19973063.2566790/150‑1 (MQ=60)
                        TTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACG                                                                                                                            <  SRR19973063.1747504/150‑1 (MQ=60)
                         TGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGG                                                                                                                           >  SRR19973063.1011498/1‑150 (MQ=60)
                          GTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGA                                                                                                                          >  SRR19973063.2737432/1‑150 (MQ=60)
                          GTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGA                                                                                                                          >  SRR19973063.4478682/1‑150 (MQ=60)
                             CGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAAGGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATA                                                                                                                       >  SRR19973063.3230721/1‑150 (MQ=60)
                             CGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATA                                                                                                                       <  SRR19973063.1042634/150‑1 (MQ=60)
                               TTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACC                                                                                                                     >  SRR19973063.4048894/1‑150 (MQ=60)
                                TATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCG                                                                                                                    >  SRR19973063.243128/1‑150 (MQ=60)
                                     TTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCG                                                                                                               <  SRR19973063.2833134/150‑1 (MQ=60)
                                     TTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCG                                                                                                               >  SRR19973063.282083/1‑150 (MQ=60)
                                     TTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCG                                                                                                               <  SRR19973063.3295288/150‑1 (MQ=60)
                                       CGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGT                                                                                                             <  SRR19973063.1900213/150‑1 (MQ=60)
                                       CGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGT                                                                                                             >  SRR19973063.3710936/1‑150 (MQ=60)
                                        GCTAACGCCA‑TTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTAT                                                                                                           <  SRR19973063.2010760/150‑1 (MQ=60)
                                        GCTAACGCCA‑TTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTAT                                                                                                           <  SRR19973063.4590343/150‑1 (MQ=60)
                                         CTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTAT                                                                                                           <  SRR19973063.3973539/150‑1 (MQ=60)
                                         CTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTAT                                                                                                           <  SRR19973063.3129668/150‑1 (MQ=60)
                                          TAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATC                                                                                                          >  SRR19973063.2292616/1‑150 (MQ=60)
                                          TAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATC                                                                                                          >  SRR19973063.303569/1‑150 (MQ=60)
                                          TAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATC                                                                                                          >  SRR19973063.211125/1‑150 (MQ=60)
                                          TAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATC                                                                                                          >  SRR19973063.211053/1‑150 (MQ=60)
                                          TAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACAGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATC                                                                                                          >  SRR19973063.4412359/1‑150 (MQ=60)
                                           AACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTCGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCT                                                                                                         >  SRR19973063.2515612/1‑150 (MQ=60)
                                             CGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTT                                                                                                       >  SRR19973063.2998972/1‑150 (MQ=60)
                                             CGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTT                                                                                                       >  SRR19973063.2998968/1‑150 (MQ=60)
                                              GCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTC                                                                                                      >  SRR19973063.2027923/1‑150 (MQ=60)
                                              GCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTC                                                                                                      >  SRR19973063.398051/1‑150 (MQ=60)
                                              GCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTC                                                                                                      >  SRR19973063.2027932/1‑150 (MQ=60)
                                              GCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTC                                                                                                      >  SRR19973063.2027973/1‑150 (MQ=60)
                                              GCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTC                                                                                                      >  SRR19973063.3391740/1‑150 (MQ=60)
                                               CCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCG                                                                                                     <  SRR19973063.2405004/150‑1 (MQ=60)
                                               CCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCG                                                                                                     >  SRR19973063.1134635/1‑150 (MQ=60)
                                                   TTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGAT                                                                                                 >  SRR19973063.1819954/1‑150 (MQ=60)
                                                   TTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGAT                                                                                                 >  SRR19973063.1819852/1‑150 (MQ=60)
                                                   TTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGAT                                                                                                 <  SRR19973063.1271396/150‑1 (MQ=60)
                                                    TGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATG                                                                                                <  SRR19973063.1696361/150‑1 (MQ=60)
                                                    TGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATG                                                                                                >  SRR19973063.523140/1‑150 (MQ=60)
                                                       GGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTC                                                                                             >  SRR19973063.3984373/1‑150 (MQ=60)
                                                         GTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCAC                                                                                           >  SRR19973063.760794/1‑150 (MQ=60)
                                                           TATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTT                                                                                         >  SRR19973063.3366864/1‑150 (MQ=60)
                                                           TATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTT                                                                                         >  SRR19973063.3364631/1‑150 (MQ=60)
                                                            ATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTT                                                                                        >  SRR19973063.3355624/1‑150 (MQ=60)
                                                               GAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACC                                                                                     >  SRR19973063.4753676/1‑150 (MQ=60)
                                                               GAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACC                                                                                     <  SRR19973063.3034424/150‑1 (MQ=60)
                                                               GAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGATTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACC                                                                                     <  SRR19973063.2563509/150‑1 (MQ=60)
                                                                 AGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCA                                                                                   >  SRR19973063.678348/1‑150 (MQ=60)
                                                                   AACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAA                                                                                 >  SRR19973063.615053/1‑150 (MQ=60)
                                                                    ACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAC                                                                                <  SRR19973063.3206706/150‑1 (MQ=60)
                                                                     CGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACG                                                                               >  SRR19973063.514116/1‑150 (MQ=60)
                                                                     CGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACG                                                                               >  SRR19973063.513986/1‑150 (MQ=60)
                                                                      GAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGC                                                                              >  SRR19973063.1420776/1‑150 (MQ=60)
                                                                      GAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCGCACTTTACCCAAACGC                                                                              >  SRR19973063.4313204/1‑150 (MQ=60)
                                                                         CGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAG                                                                           >  SRR19973063.1881512/1‑150 (MQ=60)
                                                                           GAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGA                                                                         >  SRR19973063.3971392/1‑150 (MQ=60)
                                                                            AAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGAT                                                                        >  SRR19973063.2737562/1‑150 (MQ=60)
                                                                             AGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATG                                                                       <  SRR19973063.4232318/150‑1 (MQ=60)
                                                                             AGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATG                                                                       >  SRR19973063.4177888/1‑150 (MQ=60)
                                                                             AGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATG                                                                       <  SRR19973063.2656347/150‑1 (MQ=60)
                                                                                CTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAA                                                                    <  SRR19973063.2424092/150‑1 (MQ=60)
                                                                                CTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAA                                                                    <  SRR19973063.2424139/150‑1 (MQ=60)
                                                                                 TTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAAC                                                                   <  SRR19973063.418289/150‑1 (MQ=60)
                                                                                 TTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAAC                                                                   <  SRR19973063.3095993/150‑1 (MQ=60)
                                                                                  TTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACG                                                                  <  SRR19973063.1194100/150‑1 (MQ=60)
                                                                                    GGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTC                                                                >  SRR19973063.2065477/1‑150 (MQ=60)
                                                                                    GGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTC                                                                >  SRR19973063.2065583/1‑150 (MQ=60)
                                                                                       GCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTT                                                             <  SRR19973063.897302/150‑1 (MQ=60)
                                                                                       GCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTT                                                             <  SRR19973063.897235/150‑1 (MQ=60)
                                                                                       GCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTT                                                             <  SRR19973063.2132899/150‑1 (MQ=60)
                                                                                         TAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTAC                                                           <  SRR19973063.3018080/150‑1 (MQ=60)
                                                                                         TAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTGTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTAC                                                           >  SRR19973063.1877996/1‑150 (MQ=60)
                                                                                           ATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGC                                                         <  SRR19973063.4418809/150‑1 (MQ=60)
                                                                                           ATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGC                                                         <  SRR19973063.4418763/150‑1 (MQ=60)
                                                                                               GGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGG                                                     >  SRR19973063.5087525/1‑150 (MQ=60)
                                                                                                GTAGATATCACGGCCGGGGGTCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGT                                                    >  SRR19973063.2385262/1‑150 (MQ=60)
                                                                                                  AGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTT                                                  <  SRR19973063.4509723/150‑1 (MQ=60)
                                                                                                  AGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTT                                                  >  SRR19973063.1427012/1‑150 (MQ=60)
                                                                                                  AGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTT                                                  <  SRR19973063.1627254/150‑1 (MQ=60)
                                                                                                  AGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTT                                                  <  SRR19973063.1627241/150‑1 (MQ=60)
                                                                                                  AGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTT                                                  >  SRR19973063.1427101/1‑150 (MQ=60)
                                                                                                   GATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTC                                                 <  SRR19973063.3081416/150‑1 (MQ=60)
                                                                                                   GATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTC                                                 <  SRR19973063.751460/150‑1 (MQ=60)
                                                                                                      ATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTT                                              <  SRR19973063.1994651/150‑1 (MQ=60)
                                                                                                        CACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTAT                                            >  SRR19973063.3183348/1‑150 (MQ=60)
                                                                                                        CACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTAT                                            <  SRR19973063.4671717/150‑1 (MQ=60)
                                                                                                        CACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTAT                                            <  SRR19973063.4672217/150‑1 (MQ=60)
                                                                                                         ACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATC                                           <  SRR19973063.3015678/150‑1 (MQ=60)
                                                                                                          CGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCA                                          <  SRR19973063.1709636/150‑1 (MQ=60)
                                                                                                          CGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCA                                          <  SRR19973063.1709632/150‑1 (MQ=60)
                                                                                                           GGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAA                                         >  SRR19973063.5179475/1‑150 (MQ=60)
                                                                                                             CCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGC                                       >  SRR19973063.3673537/1‑150 (MQ=60)
                                                                                                               GGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGGGGTTTCTTTATCAAGCAC                                     >  SRR19973063.1700451/1‑150 (MQ=60)
                                                                                                                  GATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTC                                  <  SRR19973063.3773726/150‑1 (MQ=60)
                                                                                                                  GATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTC                                  <  SRR19973063.1184930/150‑1 (MQ=60)
                                                                                                                    TCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGA                                <  SRR19973063.1841428/150‑1 (MQ=60)
                                                                                                                    TCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGA                                <  SRR19973063.3474057/150‑1 (MQ=60)
                                                                                                                    TCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGA                                <  SRR19973063.884983/150‑1 (MQ=60)
                                                                                                                       ACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACAC                             >  SRR19973063.3787996/1‑150 (MQ=60)
                                                                                                                           GGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATT                         <  SRR19973063.2297963/150‑1 (MQ=60)
                                                                                                                           GGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATT                         <  SRR19973063.4418428/150‑1 (MQ=60)
                                                                                                                           GGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATT                         <  SRR19973063.2298016/150‑1 (MQ=60)
                                                                                                                            GTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTC                        >  SRR19973063.2723719/1‑150 (MQ=60)
                                                                                                                            GTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTC                        >  SRR19973063.2723645/1‑150 (MQ=60)
                                                                                                                            GTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCGTACGCGTGGTTTCTTTATCAAGCACCTCGACACCATTC                        >  SRR19973063.2723736/1‑150 (MQ=60)
                                                                                                                                CATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGAC                    >  SRR19973063.2903436/1‑150 (MQ=60)
                                                                                                                                CATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGAC                    >  SRR19973063.2903430/1‑150 (MQ=60)
                                                                                                                                 ATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACG                   <  SRR19973063.85890/150‑1 (MQ=60)
                                                                                                                                 ATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACG                   >  SRR19973063.125899/1‑150 (MQ=60)
                                                                                                                                 ATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACG                   >  SRR19973063.1879956/1‑150 (MQ=60)
                                                                                                                                  TTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCTTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGT                  <  SRR19973063.4107574/150‑1 (MQ=60)
                                                                                                                                  TTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGT                  >  SRR19973063.604073/1‑150 (MQ=60)
                                                                                                                                   TAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTA                 >  SRR19973063.3950167/1‑150 (MQ=60)
                                                                                                                                     ACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAAC               <  SRR19973063.4239530/150‑1 (MQ=60)
                                                                                                                                     ACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAAC               <  SRR19973063.823850/150‑1 (MQ=60)
                                                                                                                                       CGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTG             >  SRR19973063.933587/1‑150 (MQ=60)
                                                                                                                                       CGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTG             >  SRR19973063.1041480/1‑150 (MQ=60)
                                                                                                                                          CATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTT          >  SRR19973063.4703981/1‑150 (MQ=60)
                                                                                                                                            TGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAC        >  SRR19973063.4198907/1‑150 (MQ=60)
                                                                                                                                              ATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGC      >  SRR19973063.4738250/1‑150 (MQ=60)
                                                                                                                                                  TCTTATATTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGAGGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCG  <  SRR19973063.4632049/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
GTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCG  >  CP009273/667199‑667494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑