Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F218 I0 R1	1506	278.7	9275112	94.5%	8764980	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	667,348	G→T	100%	Q99K (CAG→AAG)	lptE ←	LPS assembly OM complex LptDE, lipoprotein component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	667,348	0	G	T	95.1%	828.8 / 32.4	263	Q99K (CAG→AAG)	lptE	LPS assembly OM complex LptDE, lipoprotein component
Reads supporting (aligned to +/- strand): ref base G (7/6); new base T (139/111); total (146/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 264 total aligned reads displayed.

ACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCG  >  CP009273/667201‑667494

    ctctTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATActc                                                                                                                                              >  1:3283245/1‑150 (MQ=255)

      ctTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTctg                                                                                                                                            >  1:3880642/1‑150 (MQ=255)

       ttttACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTctgc                                                                                                                                           >  2:1841343/1‑150 (MQ=255)

         ttACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTctgctg                                                                                                                                         <  2:2554098/150‑1 (MQ=255)

         ttACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTctgctg                                                                                                                                         <  1:1548534/150‑1 (MQ=255)

          tACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGCACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGt                                                                                                                                        <  1:2064769/150‑1 (MQ=255)

           aCGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGtt                                                                                                                                       <  1:575223/150‑1 (MQ=255)

            cGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATGTGCGGGTTATCGACGAACGAACGGAAGACTTTGGCGCTACTCGGGTAGTTATCACGGCCGGGGAGCAACACGGTCGCAGTAACCGTCATGATCATCTTATACGCTGCTGtgt                                                                                                                                      >  1:3928053/1‑148 (MQ=255)

              aTCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGa                                                                                                                                    <  1:2188062/150‑1 (MQ=255)

               tCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGAc                                                                                                                                   >  1:1762222/1‑150 (MQ=255)

               tCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGAc                                                                                                                                   >  2:459359/1‑150 (MQ=255)

               tCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGAc                                                                                                                                   <  1:2411927/150‑1 (MQ=255)

                     cTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTAc                                                                                                                             <  2:900596/150‑1 (MQ=255)

                      ttGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACg                                                                                                                            <  2:298547/150‑1 (MQ=255)

                        gTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGa                                                                                                                          >  1:2007811/1‑150 (MQ=255)

                        gTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGa                                                                                                                          <  2:715901/150‑1 (MQ=255)

                          tCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAAt                                                                                                                        <  2:218711/150‑1 (MQ=255)

                            gTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCATGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATAc                                                                                                                      >  2:1332804/1‑150 (MQ=255)

                            gTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATAc                                                                                                                      >  1:462798/1‑150 (MQ=255)

                              tATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCg                                                                                                                    <  2:283366/150‑1 (MQ=255)

                                tCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGAt                                                                                                                  >  2:3235958/1‑150 (MQ=255)

                                 cTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATg                                                                                                                 <  1:3942388/150‑1 (MQ=255)

                                   ttCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCg                                                                                                               >  2:2329172/1‑150 (MQ=255)

                                   ttCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCg                                                                                                               <  2:4246796/150‑1 (MQ=255)

                                    tCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGTCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCgg                                                                                                              >  2:1439294/1‑150 (MQ=255)

                                     cGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGt                                                                                                             <  2:1748649/150‑1 (MQ=255)

                                       cTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGCTCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTAt                                                                                                           >  2:3874300/1‑150 (MQ=255)

                                       cTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTAt                                                                                                           <  2:3326660/150‑1 (MQ=255)

                                        tAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATc                                                                                                          >  2:2679407/1‑150 (MQ=255)

                                        tAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATc                                                                                                          >  1:1380895/1‑150 (MQ=255)

                                          ccGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCtt                                                                                                        <  2:838676/149‑1 (MQ=255)

                                            gCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTc                                                                                                      <  2:1911334/150‑1 (MQ=255)

                                            gCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTc                                                                                                      <  1:1027162/150‑1 (MQ=255)

                                              cATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTcgc                                                                                                    >  2:865148/1‑150 (MQ=255)

                                               aTTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTcgct                                                                                                   <  2:4029411/150‑2 (MQ=255)

                                                tttGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGa                                                                                                  >  2:3446402/1‑150 (MQ=255)

                                                 ttGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTAt                                                                                                 <  1:985572/150‑1 (MQ=255)

                                                    cGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCt                                                                                              <  2:558153/150‑1 (MQ=255)

                                                    cGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCt                                                                                              >  2:3982335/1‑150 (MQ=255)

                                                    cGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCt                                                                                              <  2:1685443/150‑1 (MQ=255)

                                                    cGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTGTCGCTATGCt                                                                                              >  1:4310806/1‑150 (MQ=255)

                                                     gggTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTc                                                                                             <  1:1007975/150‑1 (MQ=255)

                                                      ggTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCa                                                                                            <  1:1058463/150‑1 (MQ=255)

                                                         tATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACta                                                                                         >  2:2306001/1‑149 (MQ=255)

                                                          aTCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACttt                                                                                        <  1:884134/150‑1 (MQ=255)

                                                           tCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTa                                                                                       >  2:2541148/1‑150 (MQ=255)

                                                            cGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTAc                                                                                      <  1:363966/150‑1 (MQ=255)

                                                            cGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTAc                                                                                      >  2:3385842/1‑150 (MQ=255)

                                                             gaagaaCGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTAcc                                                                                     >  2:4477683/1‑150 (MQ=255)

                                                              aagaaCGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTAccc                                                                                    >  1:2837648/1‑150 (MQ=255)

                                                              aagaaCGAACGGAAGACTGTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGCCCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTAccc                                                                                    <  2:4405720/150‑1 (MQ=255)

                                                                gaacgaacGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGACTACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCaa                                                                                  >  1:3068935/1‑150 (MQ=255)

                                                                       cgGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAg                                                                           >  2:1627366/1‑150 (MQ=255)

                                                                          aaGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGAt                                                                        <  2:2470998/150‑1 (MQ=255)

                                                                            gACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATgg                                                                      >  2:1258299/1‑150 (MQ=255)

                                                                               tttGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAAc                                                                   <  2:2991235/150‑1 (MQ=255)

                                                                               tttGGCGCTAATCGGGTAGATAGCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAAc                                                                   <  1:2382553/150‑1 (MQ=255)

                                                                                ttGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACg                                                                  >  2:1077170/1‑150 (MQ=255)

                                                                                 tGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGt                                                                 >  2:2607840/1‑150 (MQ=255)

                                                                                    cgcTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCt                                                              >  2:3209261/1‑150 (MQ=255)

                                                                                     gcTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCtt                                                             <  2:4434849/150‑1 (MQ=255)

                                                                                     gcTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCtt                                                             <  1:2701289/150‑1 (MQ=255)

                                                                                       tAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTAc                                                           >  1:4188130/1‑150 (MQ=255)

                                                                                       tAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTAc                                                           >  2:3371781/1‑150 (MQ=255)

                                                                                        aaTCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTAcg                                                          >  1:535309/1‑150 (MQ=255)

                                                                                        aaTCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTAcg                                                          <  2:2854928/150‑1 (MQ=255)

                                                                                        aaTCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTAcg                                                          >  1:2847953/1‑150 (MQ=255)

                                                                                           cGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGt                                                       >  1:1920629/1‑150 (MQ=255)

                                                                                            gggTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTg                                                      <  1:4037284/150‑1 (MQ=255)

                                                                                            gggTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTg                                                      >  2:1780041/1‑150 (MQ=255)

                                                                                             ggTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTgg                                                     >  1:119786/1‑150 (MQ=255)

                                                                                             ggTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTgg                                                     <  1:2239030/150‑1 (MQ=255)

                                                                                               tAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGtt                                                   <  1:1582636/150‑1 (MQ=255)

                                                                                               tAGATATCACGGCCGGGGATCAACAAGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGtt                                                   <  1:2573791/150‑1 (MQ=255)

                                                                                                  atatCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCt                                                <  1:3739816/150‑1 (MQ=255)

                                                                                                        cGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCa                                          >  1:1703447/1‑150 (MQ=255)

                                                                                                          gCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAg                                        <  2:4345196/150‑1 (MQ=255)

                                                                                                             ggggATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCaa                                     >  1:1254293/1‑150 (MQ=255)

                                                                                                             ggggATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCaa                                     <  1:2130235/150‑1 (MQ=255)

                                                                                                               ggATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACt                                   <  1:997134/150‑1 (MQ=255)

                                                                                                               ggATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTCTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACt                                   <  1:437256/150‑1 (MQ=255)

                                                                                                                 aTCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCg                                 >  1:4467340/1‑150 (MQ=255)

                                                                                                                    aaCACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGaca                              <  1:144389/150‑1 (MQ=255)

                                                                                                                    aaCACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGaca                              <  2:821082/150‑1 (MQ=255)

                                                                                                                      cacGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACAcc                            >  2:792016/1‑150 (MQ=255)

                                                                                                                         ggTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCAtt                         >  2:3440626/1‑150 (MQ=255)

                                                                                                                           tCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCa                       <  2:220259/150‑1 (MQ=255)

                                                                                                                              cATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGAc                    <  2:4558559/150‑1 (MQ=255)

                                                                                                                               aTTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACg                   <  2:4190920/150‑1 (MQ=255)

                                                                                                                               aTTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACg                   >  1:334981/1‑150 (MQ=255)

                                                                                                                                 tAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTa                 >  2:1327365/1‑150 (MQ=255)

                                                                                                                                 tAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTa                 >  1:3162168/1‑150 (MQ=255)

                                                                                                                                 tAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTa                 >  1:3162390/1‑150 (MQ=255)

                                                                                                                                  aaCCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTaa                >  1:787759/1‑150 (MQ=255)

                                                                                                                                   aCCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAAc               >  2:1866963/1‑150 (MQ=255)

                                                                                                                                    ccGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACt              <  1:4413945/150‑1 (MQ=255)

                                                                                                                                        cATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGtt          >  1:4518680/1‑150 (MQ=255)

                                                                                                                                           gATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAcg       >  2:3660427/1‑150 (MQ=255)

                                                                                                                                             tcatcTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAcgca     >  1:1073088/1‑150 (MQ=255)

                                                                                                                                              catcTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTATCGCTATGCTCACTTTAACCAAACTCAAGGATGGAACGTCCTTACGCGAGGTTTCTTTATCACGCAACTCGACACCATTCAGACGTAACTGGTTAcgcac    >  2:2042815/1‑150 (MQ=255)

ACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCG  >  CP009273/667201‑667494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCAC  >  CP009273/667199‑667497

TTACATCTCTTTTACGTTCATGTCTTGTTCGTTATCTTTCGCTAACGCCTTTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTT                                                                                                                                                       <  SRR19973073.3508608/150‑1 (MQ=60)

GTAGATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGTGTTATCGAAGAACGAACGGACGACTTTGGCGCTAATCGGGTAGTTATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTT                                                                                                                                                       <  SRR19973073.3508611/150‑1 (MQ=60)

GTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTT                                                                                                                                                       >  SRR19973073.1313029/1‑150 (MQ=60)

GTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTT                                                                                                                                                       <  SRR19973073.1393686/150‑1 (MQ=60)

GTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTT                                                                                                                                                       <  SRR19973073.1425847/150‑1 (MQ=60)

GTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTT                                                                                                                                                       <  SRR19973073.2864379/150‑1 (MQ=60)

GTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTT                                                                                                                                                       <  SRR19973073.3142972/150‑1 (MQ=60)

GTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTT                                                                                                                                                       <  SRR19973073.1064099/150‑1 (MQ=60)

GTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTT                                                                                                                                                       >  SRR19973073.3900652/1‑150 (MQ=60)

GTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTT                                                                                                                                                       >  SRR19973073.3900447/1‑150 (MQ=60)

  ACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTAT                                                                                                                                                     >  SRR19973073.4437213/1‑150 (MQ=60)

      CTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTC                                                                                                                                                 >  SRR19973073.3289895/1‑150 (MQ=60)

        CTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTG                                                                                                                                               >  SRR19973073.568768/1‑150 (MQ=60)

        CTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTG                                                                                                                                               >  SRR19973073.3888410/1‑150 (MQ=60)

        CTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTG                                                                                                                                               >  SRR19973073.3888336/1‑150 (MQ=60)

           TTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTG                                                                                                                                            <  SRR19973073.1551700/150‑1 (MQ=60)

            TACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGCACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGT                                                                                                                                           <  SRR19973073.2069069/150‑1 (MQ=60)

            TACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGT                                                                                                                                           <  SRR19973073.2069136/150‑1 (MQ=60)

            TACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGT                                                                                                                                           >  SRR19973073.592639/1‑150 (MQ=60)

             ACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTT                                                                                                                                          <  SRR19973073.576455/150‑1 (MQ=60)

              CGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTCACCGTCATGATCATCTTATACTCTGCTGTTT                                                                                                                                         >  SRR19973073.3935874/1‑150 (MQ=60)

              CGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTT                                                                                                                                         >  SRR19973073.3935845/1‑150 (MQ=60)

              CGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTT                                                                                                                                         >  SRR19973073.3936225/1‑150 (MQ=60)

              CGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATGTGCGGGTTATCGACGAACGAACGGAAGACTTTGGCGCTACTCGGGTAGTTATCACGGCCGGGGAGCAACACGGTCGCAGTAACCGTCATGATCATCTTATACGCTGCTGTGT                                                                                                                                         >  SRR19973073.3935871/1‑150 (MQ=60)

                ATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGA                                                                                                                                       <  SRR19973073.2192702/150‑1 (MQ=60)

                ATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGA                                                                                                                                       <  SRR19973073.2192540/150‑1 (MQ=60)

                ATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGA                                                                                                                                       <  SRR19973073.2192626/150‑1 (MQ=60)

                ATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTGTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGA                                                                                                                                       >  SRR19973073.3635425/1‑150 (MQ=60)

                 TCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGAC                                                                                                                                      >  SRR19973073.1765871/1‑150 (MQ=60)

                 TCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGAC                                                                                                                                      <  SRR19973073.2416892/150‑1 (MQ=60)

                    TGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGT                                                                                                                                   >  SRR19973073.2410502/1‑150 (MQ=60)

                       CTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTAC                                                                                                                                <  SRR19973073.2069165/150‑1 (MQ=60)

                          GTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGA                                                                                                                             >  SRR19973073.2011983/1‑150 (MQ=60)

                              GTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATAC                                                                                                                         >  SRR19973073.1504368/1‑150 (MQ=60)

                              GTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATAC                                                                                                                         >  SRR19973073.463763/1‑150 (MQ=60)

                              GTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTGACGGAATAC                                                                                                                         >  SRR19973073.4302552/1‑150 (MQ=60)

                                TATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCG                                                                                                                       >  SRR19973073.233190/1‑150 (MQ=60)

                                  TCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGAT                                                                                                                     <  SRR19973073.3277063/150‑1 (MQ=60)

                                   CTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATG                                                                                                                    <  SRR19973073.3950240/150‑1 (MQ=60)

                                        GCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTA                                                                                                               <  SRR19973073.1657511/150‑1 (MQ=60)

                                        GCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTA                                                                                                               >  SRR19973073.2756604/1‑150 (MQ=60)

                                        GCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTA                                                                                                               >  SRR19973073.2756627/1‑150 (MQ=60)

                                         CTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTAT                                                                                                              >  SRR19973073.2549641/1‑150 (MQ=60)

                                          TAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATC                                                                                                             >  SRR19973073.1383819/1‑150 (MQ=60)

                                          TAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATC                                                                                                             >  SRR19973073.1161809/1‑150 (MQ=60)

                                           AACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGTGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCT                                                                                                            >  SRR19973073.1439749/1‑150 (MQ=60)

                                            ACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTT                                                                                                           <  SRR19973073.4585608/150‑1 (MQ=60)

                                            ACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTT                                                                                                           <  SRR19973073.4585584/150‑1 (MQ=60)

                                            ACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTT                                                                                                           <  SRR19973073.1222477/150‑1 (MQ=60)

                                             CGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTT                                                                                                          >  SRR19973073.2961203/1‑150 (MQ=60)

                                             CGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTT                                                                                                          <  SRR19973073.1050491/150‑1 (MQ=60)

                                              GCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTC                                                                                                         <  SRR19973073.1029306/150‑1 (MQ=60)

                                                 ATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCT                                                                                                      >  SRR19973073.1757092/1‑150 (MQ=60)

                                                   TTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTAT                                                                                                    <  SRR19973073.987626/150‑1 (MQ=60)

                                                   TTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTAT                                                                                                    <  SRR19973073.987627/150‑1 (MQ=60)

                                                   TTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTAT                                                                                                    <  SRR19973073.987913/150‑1 (MQ=60)

                                                   GTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGAT                                                                                                    <  SRR19973073.102778/150‑1 (MQ=60)

                                                     GCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGC                                                                                                  >  SRR19973073.2613263/1‑150 (MQ=60)

                                                      CGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTGTCGCTATGCT                                                                                                 >  SRR19973073.4319402/1‑150 (MQ=60)

                                                       GGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTC                                                                                                <  SRR19973073.4381809/150‑1 (MQ=60)

                                                       GGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTC                                                                                                <  SRR19973073.1010082/150‑1 (MQ=60)

                                                        GGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCA                                                                                               <  SRR19973073.1060658/150‑1 (MQ=60)

                                                        GGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCA                                                                                               <  SRR19973073.1060576/150‑1 (MQ=60)

                                                           TATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCCCGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTT                                                                                            >  SRR19973073.2402623/1‑150 (MQ=60)

                                                            ATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTT                                                                                           <  SRR19973073.1934393/150‑1 (MQ=60)

                                                            ATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTT                                                                                           <  SRR19973073.885959/150‑1 (MQ=60)

                                                              CGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTAC                                                                                         <  SRR19973073.4505104/150‑1 (MQ=60)

                                                              CGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTAC                                                                                         <  SRR19973073.364692/150‑1 (MQ=60)

                                                                AAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCC                                                                                       >  SRR19973073.1261165/1‑150 (MQ=60)

                                                                AAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCC                                                                                       >  SRR19973073.2843467/1‑150 (MQ=60)

                                                                 AGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCA                                                                                      >  SRR19973073.157025/1‑150 (MQ=60)

                                                                  GAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGACTACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAA                                                                                     >  SRR19973073.3075110/1‑150 (MQ=60)

                                                                      GAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGC                                                                                 >  SRR19973073.4509456/1‑150 (MQ=60)

                                                                        ACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAA                                                                               <  SRR19973073.202842/150‑1 (MQ=60)

                                                                          GGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGG                                                                             >  SRR19973073.2261384/1‑150 (MQ=60)

                                                                           GAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGA                                                                            <  SRR19973073.3992147/150‑1 (MQ=60)

                                                                              GACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGG                                                                         >  SRR19973073.4278360/1‑150 (MQ=60)

                                                                                 TTTGGCGCTAATCGGGTAGATAGCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAAC                                                                      <  SRR19973073.2387436/150‑1 (MQ=60)

                                                                                       GCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTT                                                                <  SRR19973073.2706804/150‑1 (MQ=60)

                                                                                         TAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTAC                                                              >  SRR19973073.4196452/1‑150 (MQ=60)

                                                                                         TAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTAC                                                              >  SRR19973073.3111603/1‑150 (MQ=60)

                                                                                         TAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTAC                                                              >  SRR19973073.1763831/1‑150 (MQ=60)

                                                                                         TAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTAC                                                              <  SRR19973073.1622615/150‑1 (MQ=60)

                                                                                          AATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACG                                                             >  SRR19973073.1076324/1‑150 (MQ=60)

                                                                                          AATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACG                                                             >  SRR19973073.536291/1‑150 (MQ=60)

                                                                                          AATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACG                                                             >  SRR19973073.1489834/1‑150 (MQ=60)

                                                                                          AATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACG                                                             >  SRR19973073.536464/1‑150 (MQ=60)

                                                                                          AATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACG                                                             >  SRR19973073.2853787/1‑150 (MQ=60)

                                                                                          AATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACATTACACAAACGCAAGGATGGAACGTCCTTACG                                                             >  SRR19973073.2853716/1‑150 (MQ=60)

                                                                                           ATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGC                                                            >  SRR19973073.746830/1‑150 (MQ=60)

                                                                                             CGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGT                                                          >  SRR19973073.1924538/1‑150 (MQ=60)

                                                                                              GGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTG                                                         <  SRR19973073.4045356/150‑1 (MQ=60)

                                                                                               GGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGG                                                        <  SRR19973073.2164858/150‑1 (MQ=60)

                                                                                               GGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGG                                                        >  SRR19973073.120072/1‑150 (MQ=60)

                                                                                               GGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGG                                                        <  SRR19973073.2243647/150‑1 (MQ=60)

                                                                                                GTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGT                                                       >  SRR19973073.3333037/1‑150 (MQ=60)

                                                                                                 TAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTT                                                      <  SRR19973073.1585900/150‑1 (MQ=60)

                                                                                                 TAGATATCACGGCCGGGGATCAACAAGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTT                                                      <  SRR19973073.2579095/150‑1 (MQ=60)

                                                                                                  AGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTT                                                     >  SRR19973073.4106019/1‑150 (MQ=60)

                                                                                                    ATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCT                                                   <  SRR19973073.3747324/150‑1 (MQ=60)

                                                                                                    ATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCT                                                   <  SRR19973073.1423381/150‑1 (MQ=60)

                                                                                                      ATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTT                                                 <  SRR19973073.3678088/150‑1 (MQ=60)

                                                                                                         ACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATC                                              <  SRR19973073.2704724/150‑1 (MQ=60)

                                                                                                          CGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCA                                             >  SRR19973073.1706981/1‑150 (MQ=60)

                                                                                                           GGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAA                                            >  SRR19973073.3461218/1‑150 (MQ=60)

                                                                                                              CGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCA                                         >  SRR19973073.3273491/1‑150 (MQ=60)

                                                                                                               GGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAA                                        >  SRR19973073.3092200/1‑150 (MQ=60)

                                                                                                               GGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAA                                        >  SRR19973073.1256915/1‑150 (MQ=60)

                                                                                                               GGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAA                                        <  SRR19973073.2134695/150‑1 (MQ=60)

                                                                                                                 GGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACT                                      <  SRR19973073.999218/150‑1 (MQ=60)

                                                                                                                 GGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACT                                      <  SRR19973073.3714721/150‑1 (MQ=60)

                                                                                                                 GGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTCTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACT                                      <  SRR19973073.438141/150‑1 (MQ=60)

                                                                                                                  GATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTC                                     >  SRR19973073.1071765/1‑150 (MQ=60)

                                                                                                                   ATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCG                                    >  SRR19973073.4476323/1‑150 (MQ=60)

                                                                                                                   ATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCG                                    >  SRR19973073.4350756/1‑150 (MQ=60)

                                                                                                                     CAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGAC                                  >  SRR19973073.905863/1‑150 (MQ=60)

                                                                                                                      AACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACA                                 <  SRR19973073.144737/150‑1 (MQ=60)

                                                                                                                           GGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATT                            >  SRR19973073.1950888/1‑150 (MQ=60)

                                                                                                                                 ATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACG                      >  SRR19973073.335664/1‑150 (MQ=60)

                                                                                                                                 ATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACG                      >  SRR19973073.934478/1‑150 (MQ=60)

                                                                                                                                   TAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTA                    >  SRR19973073.3168537/1‑150 (MQ=60)

                                                                                                                                   TAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTA                    >  SRR19973073.3168561/1‑150 (MQ=60)

                                                                                                                                   TAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTA                    >  SRR19973073.3168759/1‑150 (MQ=60)

                                                                                                                                    AACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAA                   >  SRR19973073.789347/1‑150 (MQ=60)

                                                                                                                                    AACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAA                   >  SRR19973073.789362/1‑150 (MQ=60)

                                                                                                                                      CCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACT                 <  SRR19973073.4422801/150‑1 (MQ=60)

                                                                                                                                      CCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACT                 <  SRR19973073.4422788/150‑1 (MQ=60)

                                                                                                                                      CCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACT                 <  SRR19973073.557736/150‑1 (MQ=60)

                                                                                                                                         TCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGT              >  SRR19973073.1241730/1‑150 (MQ=60)

                                                                                                                                          CATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTT             >  SRR19973073.4527749/1‑150 (MQ=60)

                                                                                                                                           ATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTA            >  SRR19973073.4061876/1‑150 (MQ=60)

                                                                                                                                            TGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAC           <  SRR19973073.3808849/150‑1 (MQ=60)

                                                                                                                                               TCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCA        >  SRR19973073.1075304/1‑150 (MQ=60)

                                                                                                                                                CATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCAC       >  SRR19973073.1911637/1‑150 (MQ=60)

                                                                                                                                                  TCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCG     <  SRR19973073.3024719/150‑1 (MQ=60)

                                                                                                                                                    TTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCA   >  SRR19973073.326151/1‑150 (MQ=60)

                                                                                                                                                    TTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCA   >  SRR19973073.2434285/1‑150 (MQ=60)

                                                                                                                                                    TTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCTATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCA   >  SRR19973073.1057590/1‑150 (MQ=60)

                                                                                                                                                    TTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCA   <  SRR19973073.4113111/150‑1 (MQ=60)

                                                                                                                                                    TTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCA   <  SRR19973073.1024510/150‑1 (MQ=60)

                                                                                                                                                     TATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCAC  <  SRR19973073.3235059/150‑1 (MQ=60)

                                                                                                                                                     TATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCAC  <  SRR19973073.3235056/150‑1 (MQ=60)

                                                                                                                                                     TATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCAC  <  SRR19973073.2274581/150‑1 (MQ=60)

                                                                                                                                                     TATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCAC  >  SRR19973073.158233/1‑150 (MQ=60)

GTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCAC  >  CP009273/667199‑667497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`