Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 748,707 A→T 100% I406I (ATT→ATA)  gltA ← citrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273748,7070AT99.2% 780.6 / ‑2.7 235I406I (ATT→ATA) gltAcitrate synthase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (134/99);  total (135/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 235 total aligned reads displayed.

TTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACA  >  CP009273/748564‑748852
                                                                                                                                               |                                                                                                                                                 
ttttGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTc                                                                                                                                             >  1:3464064/1‑150 (MQ=255)
ttttGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTc                                                                                                                                             <  1:1594496/150‑1 (MQ=255)
   tgtgTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATa                                                                                                                                          >  2:2115433/1‑150 (MQ=255)
   tgtgTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATa                                                                                                                                          >  1:2292773/1‑150 (MQ=255)
     tgtTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATAcc                                                                                                                                        >  2:121615/1‑150 (MQ=255)
     tgtTTAGGCATATAAAAATCAACCCGCCAGATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATAcc                                                                                                                                        <  2:2983335/150‑1 (MQ=255)
      gtTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCg                                                                                                                                       >  1:4298134/1‑150 (MQ=255)
        ttAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTc                                                                                                                                     >  1:1923835/1‑150 (MQ=255)
        ttAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTc                                                                                                                                     <  1:148058/150‑1 (MQ=255)
          aGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCAc                                                                                                                                   <  1:1619012/150‑1 (MQ=255)
           ggCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACt                                                                                                                                  <  1:4268712/150‑1 (MQ=255)
           ggCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACt                                                                                                                                  >  2:3873847/1‑150 (MQ=255)
           ggCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACt                                                                                                                                  <  2:4701470/150‑1 (MQ=255)
                    aaaTCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTc                                                                                                                         >  2:4184476/1‑150 (MQ=255)
                    aaaTCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTc                                                                                                                         <  2:5133070/150‑1 (MQ=255)
                       tCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCt                                                                                                                      <  1:2364104/150‑1 (MQ=255)
                        cAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTc                                                                                                                     <  2:2412250/150‑1 (MQ=255)
                         aaCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTGCGCTcc                                                                                                                    >  2:1810597/1‑150 (MQ=255)
                            ccGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGt                                                                                                                 <  2:335607/150‑1 (MQ=255)
                             tgCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTg                                                                                                                >  2:4007666/2‑150 (MQ=255)
                             tgCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTg                                                                                                                >  2:1429370/2‑150 (MQ=255)
                             cGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTg                                                                                                                >  1:3811304/1‑150 (MQ=255)
                                  tatGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGa                                                                                                           >  2:2492571/1‑150 (MQ=255)
                                   atGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGAt                                                                                                          <  1:2528857/150‑1 (MQ=255)
                                   atGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGAt                                                                                                          >  2:822386/1‑150 (MQ=255)
                                    tGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATc                                                                                                         >  2:4101566/1‑150 (MQ=255)
                                      aaCGGCTGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGATTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCa                                                                                                       <  2:1567552/150‑1 (MQ=255)
                                      aaCGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCa                                                                                                       >  1:1805706/1‑150 (MQ=255)
                                         ggcggGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGcc                                                                                                    >  2:3131788/1‑150 (MQ=255)
                                               ttAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATACTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGt                                                                                              >  1:1357130/1‑150 (MQ=255)
                                                   aaTATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTGTAAAGTCGCGTTTTTCAAATCATGTATACAGCTGACGCGGACGGGCTCTCTTCATACCGTACCTGTGCATTTCGCGGGAGTGGGAGAGCCAGCGAACGGTGCgg                                                                                          >  2:2949969/1‑149 (MQ=255)
                                                    atatTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTg                                                                                         >  2:18965/1‑150 (MQ=255)
                                                    atatTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTg                                                                                         <  2:3687358/150‑1 (MQ=255)
                                                     tatTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGc                                                                                        <  1:3569045/150‑1 (MQ=255)
                                                     tatTTACAACTTAGCAATCAACCATTAACGCGTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGc                                                                                        >  1:4989299/1‑150 (MQ=255)
                                                        ttACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCAt                                                                                     >  2:2469494/1‑150 (MQ=255)
                                                        ttACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCAt                                                                                     <  1:4206148/150‑1 (MQ=255)
                                                        ttACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCAt                                                                                     <  1:1627201/150‑1 (MQ=255)
                                                         tACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCAtt                                                                                    >  1:469881/1‑150 (MQ=255)
                                                         tACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCAtt                                                                                    >  2:1004711/1‑150 (MQ=255)
                                                          aCAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTg                                                                                   <  1:1046232/150‑1 (MQ=255)
                                                            aaCTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCg                                                                                 >  1:3649016/1‑150 (MQ=255)
                                                               ttAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGaaa                                                                              >  1:3077663/1‑150 (MQ=255)
                                                                tAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGaaaa                                                                             >  1:3746703/1‑150 (MQ=255)
                                                                  gCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATg                                                                           >  2:611969/1‑150 (MQ=255)
                                                                   cAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGa                                                                          >  2:2548658/1‑150 (MQ=255)
                                                                   cAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGa                                                                          >  1:1095030/1‑150 (MQ=255)
                                                                   aaaTCAACCATTAACGCTTGATATATCTTTTAAATTCGCGTTTTTCATCTCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGa                                                                          <  2:4054569/149‑1 (MQ=255)
                                                                        aaCCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTg                                                                     >  1:816921/1‑150 (MQ=255)
                                                                          ccATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGaa                                                                   >  1:4138702/1‑150 (MQ=255)
                                                                          ccATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGaa                                                                   >  2:1805297/1‑150 (MQ=255)
                                                                             ttAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACAt                                                                <  1:2692011/150‑1 (MQ=255)
                                                                                 cGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGaa                                                            >  2:3484867/1‑150 (MQ=255)
                                                                                 cGCTAGATATCGCTGTTAAAGACGCGTTTTTCATATCCTGTATAAAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGAATTTCGCTACATTGGGCGATCAAGACAAAGGTACGTGCCAATTCGAAAAATACGGTGAACATGGaa                                                            >  2:2191770/1‑150 (MQ=255)
                                                                                  gCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAg                                                           <  1:3574760/150‑1 (MQ=255)
                                                                                  gCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAg                                                           <  1:1991851/150‑1 (MQ=255)
                                                                                  gCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAg                                                           <  1:4067142/150‑1 (MQ=255)
                                                                                    ttGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGAc                                                         >  2:4812294/1‑150 (MQ=255)
                                                                                    ttGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGAc                                                         >  2:4566163/1‑150 (MQ=255)
                                                                                     tGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACg                                                        >  2:3462225/1‑150 (MQ=255)
                                                                                         atCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAAt                                                    <  1:3332682/150‑1 (MQ=255)
                                                                                           cGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATAc                                                  <  1:1218400/150‑1 (MQ=255)
                                                                                             cTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATAccc                                                <  1:3806126/150‑1 (MQ=255)
                                                                                              ttttAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCa                                               <  2:3347104/150‑1 (MQ=255)
                                                                                                 tAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCg                                            <  1:2013490/150‑1 (MQ=255)
                                                                                                   aaGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCt                                          >  1:1278531/1‑150 (MQ=255)
                                                                                                   aaGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCt                                          <  2:2696344/150‑1 (MQ=255)
                                                                                                     gTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCttt                                        >  2:2273798/1‑150 (MQ=255)
                                                                                                      tCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTc                                       >  2:3246577/1‑150 (MQ=255)
                                                                                                       cgcgTTTTTCATATCCTGTATACAGCTGACGCTGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCa                                      <  1:4938868/150‑1 (MQ=255)
                                                                                                         cgTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAgg                                    >  2:4335565/1‑150 (MQ=255)
                                                                                                         cgTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAgg                                    >  1:5104182/1‑150 (MQ=255)
                                                                                                          gTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGga                                   <  2:1512705/150‑1 (MQ=255)
                                                                                                          gTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGga                                   >  2:1800263/1‑150 (MQ=255)
                                                                                                           tttttCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATTGAAGACGGAAGACCCATCGCTTTCAGgat                                  <  2:1893015/150‑1 (MQ=255)
                                                                                                            ttttCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGgatg                                 <  1:5140257/150‑1 (MQ=255)
                                                                                                             tttCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGgatga                                <  1:5016390/150‑1 (MQ=255)
                                                                                                              ttCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGgatgat                               <  2:2598221/150‑1 (MQ=255)
                                                                                                                  tatCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCa                           <  1:3497572/150‑1 (MQ=255)
                                                                                                                  tatCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCa                           <  1:2115433/150‑1 (MQ=255)
                                                                                                                   atCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCag                          >  1:890640/1‑150 (MQ=255)
                                                                                                                     ccTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCagag                        >  2:4193946/1‑150 (MQ=255)
                                                                                                                      cTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGt                       >  2:2706004/1‑150 (MQ=255)
                                                                                                                        gTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAg                     >  1:3976960/1‑150 (MQ=255)
                                                                                                                           taCAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTCGaaa                  >  2:2804325/1‑150 (MQ=255)
                                                                                                                           taCAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGaaa                  <  2:3741510/150‑1 (MQ=255)
                                                                                                                            aCAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAAt                 >  1:3616720/1‑150 (MQ=255)
                                                                                                                            aCAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAAt                 <  2:607451/150‑1 (MQ=255)
                                                                                                                             cAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATc                <  1:3145735/150‑1 (MQ=255)
                                                                                                                             cAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATc                >  1:435688/1‑150 (MQ=255)
                                                                                                                              aGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCg               >  1:4565418/1‑150 (MQ=255)
                                                                                                                              aGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCg               >  2:4167362/1‑150 (MQ=255)
                                                                                                                              aGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCg               >  2:2418385/1‑150 (MQ=255)
                                                                                                                                 tGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACg            <  2:833914/150‑1 (MQ=255)
                                                                                                                                 tGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACg            <  1:4248164/150‑1 (MQ=255)
                                                                                                                                 tGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACg            >  1:209081/1‑150 (MQ=255)
                                                                                                                                   aCGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGtt          <  2:4766245/150‑1 (MQ=255)
                                                                                                                                   aCGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGtt          <  2:4538993/150‑1 (MQ=255)
                                                                                                                                      cgGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCgg       >  1:1823702/1‑150 (MQ=255)
                                                                                                                                      cgGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCgg       >  1:748799/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
TTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACA  >  CP009273/748564‑748852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTT  >  CP009273/748564‑748855
                                                                                                                                               |                                                                                                                                                    
TTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTC                                                                                                                                                >  SRR19973063.53220/1‑150 (MQ=60)
TTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTC                                                                                                                                                >  SRR19973063.3475087/1‑150 (MQ=60)
TTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTC                                                                                                                                                <  SRR19973063.1599388/150‑1 (MQ=60)
 TTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCA                                                                                                                                               >  SRR19973063.2183591/1‑150 (MQ=60)
  TTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCAT                                                                                                                                              >  SRR19973063.2532786/1‑150 (MQ=60)
   TGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATA                                                                                                                                             >  SRR19973063.2300092/1‑150 (MQ=60)
     TGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACC                                                                                                                                           >  SRR19973063.3603611/1‑150 (MQ=60)
      GTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCG                                                                                                                                          >  SRR19973063.4311793/1‑150 (MQ=60)
      GTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCG                                                                                                                                          >  SRR19973063.4311778/1‑150 (MQ=60)
        TTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTACCGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTC                                                                                                                                        <  SRR19973063.3174610/150‑1 (MQ=60)
        TTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTC                                                                                                                                        >  SRR19973063.1929851/1‑150 (MQ=60)
        TTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTC                                                                                                                                        <  SRR19973063.148515/150‑1 (MQ=60)
          AGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCAC                                                                                                                                      <  SRR19973063.1623976/150‑1 (MQ=60)
          AGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCAC                                                                                                                                      <  SRR19973063.1623961/150‑1 (MQ=60)
           GGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACT                                                                                                                                     <  SRR19973063.4282281/150‑1 (MQ=60)
           GGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACT                                                                                                                                     <  SRR19973063.4282286/150‑1 (MQ=60)
                   AAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTT                                                                                                                             >  SRR19973063.4958892/1‑150 (MQ=60)
                   AAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTT                                                                                                                             <  SRR19973063.3505430/150‑1 (MQ=60)
                     AATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCG                                                                                                                           >  SRR19973063.3699164/1‑150 (MQ=60)
                       TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCT                                                                                                                         <  SRR19973063.2371679/150‑1 (MQ=60)
                       TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCT                                                                                                                         <  SRR19973063.1264216/150‑1 (MQ=60)
                       TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCT                                                                                                                         <  SRR19973063.18848/150‑1 (MQ=60)
                        CAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTC                                                                                                                        >  SRR19973063.1317242/1‑150 (MQ=60)
                         AACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCC                                                                                                                       <  SRR19973063.4624666/150‑1 (MQ=60)
                          ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCA                                                                                                                      <  SRR19973063.4045286/150‑1 (MQ=60)
                             CGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTG                                                                                                                   >  SRR19973063.3823468/1‑150 (MQ=60)
                             CGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTG                                                                                                                   >  SRR19973063.3823434/1‑150 (MQ=60)
                                  TATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGA                                                                                                              <  SRR19973063.3087124/150‑1 (MQ=60)
                                   ATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGAT                                                                                                             <  SRR19973063.2536922/150‑1 (MQ=60)
                                      AACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCA                                                                                                          >  SRR19973063.1811310/1‑150 (MQ=60)
                                      AACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCA                                                                                                          >  SRR19973063.2115748/1‑150 (MQ=60)
                                         GGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCC                                                                                                       <  SRR19973063.2347614/150‑1 (MQ=60)
                                               TTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATACTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGT                                                                                                 >  SRR19973063.1361208/1‑150 (MQ=60)
                                                TAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTA                                                                                                >  SRR19973063.3782107/1‑150 (MQ=60)
                                                     TATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGC                                                                                           <  SRR19973063.3580487/150‑1 (MQ=60)
                                                     TATTTACAACTTAGCAATCAACCATTAACGCGTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGC                                                                                           >  SRR19973063.5005418/1‑150 (MQ=60)
                                                        TTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCAT                                                                                        <  SRR19973063.4219566/150‑1 (MQ=60)
                                                        TTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCAT                                                                                        <  SRR19973063.1632172/150‑1 (MQ=60)
                                                         TACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATT                                                                                       <  SRR19973063.3822207/150‑1 (MQ=60)
                                                         TACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATT                                                                                       >  SRR19973063.471209/1‑150 (MQ=60)
                                                          ACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTG                                                                                      <  SRR19973063.1049401/150‑1 (MQ=60)
                                                            AACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCG                                                                                    >  SRR19973063.3660716/1‑150 (MQ=60)
                                                            AACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCG                                                                                    <  SRR19973063.1091338/150‑1 (MQ=60)
                                                              CTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAA                                                                                  >  SRR19973063.868344/1‑150 (MQ=60)
                                                              CTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAA                                                                                  >  SRR19973063.5025709/1‑150 (MQ=60)
                                                               TTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAA                                                                                 >  SRR19973063.3087553/1‑150 (MQ=60)
                                                                TAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAA                                                                                >  SRR19973063.1181536/1‑150 (MQ=60)
                                                                TAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAA                                                                                >  SRR19973063.3758675/1‑150 (MQ=60)
                                                                  GCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATG                                                                              <  SRR19973063.141614/150‑1 (MQ=60)
                                                                   CAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGA                                                                             >  SRR19973063.1098363/1‑150 (MQ=60)
                                                                       CAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGT                                                                         <  SRR19973063.2557898/150‑1 (MQ=60)
                                                                        AACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTG                                                                        >  SRR19973063.819326/1‑150 (MQ=60)
                                                                        AACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTG                                                                        >  SRR19973063.819283/1‑150 (MQ=60)
                                                                        AACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTG                                                                        >  SRR19973063.15738/1‑150 (MQ=60)
                                                                          CCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAA                                                                      >  SRR19973063.4151938/1‑150 (MQ=60)
                                                                            ATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACA                                                                    <  SRR19973063.4311131/150‑1 (MQ=60)
                                                                             TTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACAT                                                                   <  SRR19973063.2700683/150‑1 (MQ=60)
                                                                             TTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACAT                                                                   <  SRR19973063.2700697/150‑1 (MQ=60)
                                                                              TAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCAATGCGAAAATGACGGTGAACATG                                                                  >  SRR19973063.4318468/1‑150 (MQ=60)
                                                                               AACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGG                                                                 >  SRR19973063.1590410/1‑150 (MQ=60)
                                                                                  GCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAG                                                              <  SRR19973063.3586012/150‑1 (MQ=60)
                                                                                  GCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAG                                                              <  SRR19973063.3586174/150‑1 (MQ=60)
                                                                                  GCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAG                                                              <  SRR19973063.3586223/150‑1 (MQ=60)
                                                                                  GCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAG                                                              <  SRR19973063.3447180/150‑1 (MQ=60)
                                                                                  GCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAG                                                              <  SRR19973063.4080179/150‑1 (MQ=60)
                                                                                  GCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAG                                                              <  SRR19973063.1998078/150‑1 (MQ=60)
                                                                                     TGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACG                                                           >  SRR19973063.3520544/1‑150 (MQ=60)
                                                                                         ATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAAT                                                       >  SRR19973063.198353/1‑150 (MQ=60)
                                                                                         ATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAAT                                                       <  SRR19973063.3343312/150‑1 (MQ=60)
                                                                                           CGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATAC                                                     <  SRR19973063.1222048/150‑1 (MQ=60)
                                                                                            GCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACC                                                    >  SRR19973063.1684368/1‑150 (MQ=60)
                                                                                             CTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCC                                                   <  SRR19973063.3818245/150‑1 (MQ=60)
                                                                                                 TAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCG                                               <  SRR19973063.2019775/150‑1 (MQ=60)
                                                                                                  AAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGC                                              >  SRR19973063.758836/1‑150 (MQ=60)
                                                                                                   AAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCT                                             >  SRR19973063.1282345/1‑150 (MQ=60)
                                                                                                    AGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTT                                            >  SRR19973063.5102506/1‑150 (MQ=60)
                                                                                                     GTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTT                                           >  SRR19973063.3668064/1‑150 (MQ=60)
                                                                                                     GTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTT                                           >  SRR19973063.3430944/1‑150 (MQ=60)
                                                                                                      TCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAGATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTC                                          >  SRR19973063.3896437/1‑150 (MQ=60)
                                                                                                       CGCGTTTTTCATATCCTGTATACAGCTGACGCTGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCA                                         <  SRR19973063.4954830/150‑1 (MQ=60)
                                                                                                         CGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGG                                       >  SRR19973063.1240510/1‑150 (MQ=60)
                                                                                                         CGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGG                                       >  SRR19973063.5120609/1‑150 (MQ=60)
                                                                                                         CGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGG                                       >  SRR19973063.5120608/1‑150 (MQ=60)
                                                                                                            TTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATG                                    <  SRR19973063.5156794/150‑1 (MQ=60)
                                                                                                             TTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGA                                   <  SRR19973063.5032582/150‑1 (MQ=60)
                                                                                                                  TATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCA                              <  SRR19973063.3508754/150‑1 (MQ=60)
                                                                                                                  TATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCA                              <  SRR19973063.2122053/150‑1 (MQ=60)
                                                                                                                   ATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAG                             >  SRR19973063.893224/1‑150 (MQ=60)
                                                                                                                        GTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAG                        >  SRR19973063.3989704/1‑150 (MQ=60)
                                                                                                                        GTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAG                        >  SRR19973063.5004225/1‑150 (MQ=60)
                                                                                                                         TATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGA                       >  SRR19973063.197211/1‑150 (MQ=60)
                                                                                                                         TATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGA                       >  SRR19973063.4182128/1‑150 (MQ=60)
                                                                                                                            ACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAAT                    >  SRR19973063.3628329/1‑150 (MQ=60)
                                                                                                                            ACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAAT                    <  SRR19973063.5146828/150‑1 (MQ=60)
                                                                                                                             CAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATC                   >  SRR19973063.436897/1‑150 (MQ=60)
                                                                                                                             CAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATC                   <  SRR19973063.3155847/150‑1 (MQ=60)
                                                                                                                              AGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCG                  >  SRR19973063.2475511/1‑150 (MQ=60)
                                                                                                                              AGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCG                  >  SRR19973063.3637388/1‑150 (MQ=60)
                                                                                                                              AGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCG                  >  SRR19973063.4580032/1‑150 (MQ=60)
                                                                                                                                CTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTGGAAATCGAC                >  SRR19973063.4253992/1‑150 (MQ=60)
                                                                                                                                 TGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACG               <  SRR19973063.923100/150‑1 (MQ=60)
                                                                                                                                 TGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACG               >  SRR19973063.4498478/1‑150 (MQ=60)
                                                                                                                                 TGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACG               >  SRR19973063.209709/1‑150 (MQ=60)
                                                                                                                                 TGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACG               <  SRR19973063.4261700/150‑1 (MQ=60)
                                                                                                                                 TGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACG               >  SRR19973063.209741/1‑150 (MQ=60)
                                                                                                                                 TGACGCGGAAGGGCTATCTTCATACCGTAACTGTGAATATCTCTCCATTGTGCGATCCAGCCAACGGTACGAGCCATTGCGAAAATGACGTTGAAAATGGAAGACGGAATAACAATCGCTTTCAGGTTGATACCAGAGTAGAACTCTACG               >  SRR19973063.209480/1‑150 (MQ=60)
                                                                                                                                     GCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCG           <  SRR19973063.4114708/150‑1 (MQ=60)
                                                                                                                                     GCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCG           <  SRR19973063.3139151/150‑1 (MQ=60)
                                                                                                                                     GCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCG           <  SRR19973063.1608642/150‑1 (MQ=60)
                                                                                                                                     GCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCG           <  SRR19973063.4448062/150‑1 (MQ=60)
                                                                                                                                      CGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGG          >  SRR19973063.750985/1‑150 (MQ=60)
                                                                                                                                      CGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGG          >  SRR19973063.1829374/1‑150 (MQ=60)
                                                                                                                                       GGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGG         >  SRR19973063.169098/1‑150 (MQ=60)
                                                                                                                                        GACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGT        >  SRR19973063.4761921/1‑150 (MQ=60)
                                                                                                                                        GACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGT        >  SRR19973063.2009259/1‑150 (MQ=60)
                                                                                                                                           GGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACA     >  SRR19973063.4188862/1‑150 (MQ=60)
                                                                                                                                           GGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACA     >  SRR19973063.1827237/1‑150 (MQ=60)
                                                                                                                                              CTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTT  <  SRR19973063.2963557/150‑1 (MQ=60)
                                                                                                                                               |                                                                                                                                                    
TTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTT  >  CP009273/748564‑748855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑