Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 748,707 A→T 100% I406I (ATT→ATA)  gltA ← citrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273748,7070AT99.3% 955.6 / ‑2.7 290I406I (ATT→ATA) gltAcitrate synthase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (138/149);  minor base C (2/0);  total (141/149)
Fisher's exact test for biased strand distribution p-value = 2.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 290 total aligned reads displayed.

AATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAG  >  CP009273/748560‑748853
                                                                                                                                                   |                                                                                                                                                  
aaTTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAATTCTTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTGCATATCCTGTATACAGCTGACGCGGACGGGCTAt                                                                                                                                                  >  2:310824/1‑150 (MQ=255)
aaTTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTAt                                                                                                                                                  <  1:4711506/150‑1 (MQ=255)
  ttttttGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCt                                                                                                                                                <  1:5198754/150‑1 (MQ=255)
  ttttttGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCt                                                                                                                                                <  2:1355851/150‑1 (MQ=255)
    ttttGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTc                                                                                                                                              >  1:3704748/1‑150 (MQ=255)
    ttttGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTc                                                                                                                                              <  2:2289676/150‑1 (MQ=255)
     tttGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCa                                                                                                                                             <  1:2228731/150‑1 (MQ=255)
          gtTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCg                                                                                                                                        <  1:5266737/150‑1 (MQ=255)
           tttAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGt                                                                                                                                       <  1:794654/150‑1 (MQ=255)
             tAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCa                                                                                                                                     <  1:3835392/150‑1 (MQ=255)
              aGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCAc                                                                                                                                    >  1:3492470/1‑150 (MQ=255)
               ggCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACt                                                                                                                                   <  1:5087366/150‑1 (MQ=255)
                 cATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACtgt                                                                                                                                 <  1:1666745/150‑1 (MQ=255)
                  atatAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACtgtg                                                                                                                                >  1:1046401/1‑150 (MQ=255)
                  atatAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACtgtg                                                                                                                                >  2:4965272/1‑150 (MQ=255)
                  atatAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACtgtg                                                                                                                                >  2:2356504/1‑150 (MQ=255)
                    ataAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCa                                                                                                                              >  2:2870822/1‑150 (MQ=255)
                    ataAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCa                                                                                                                              >  1:34617/1‑150 (MQ=255)
                      aaaaaTCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCAtt                                                                                                                            <  2:1944388/150‑1 (MQ=255)
                         aaTCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCg                                                                                                                         >  1:2396369/1‑150 (MQ=255)
                           tCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCt                                                                                                                       <  1:1373494/150‑1 (MQ=255)
                              aCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCa                                                                                                                    <  1:1575819/150‑1 (MQ=255)
                                 cGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTg                                                                                                                 <  1:1740023/150‑1 (MQ=255)
                                  gCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTgg                                                                                                                >  1:827238/1‑150 (MQ=255)
                                   ccATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTggg                                                                                                               >  2:305146/1‑150 (MQ=255)
                                    cATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATAAACCATAAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGACACAGTGCATATCGCTCCAGTGGGc                                                                                                              >  2:644406/1‑150 (MQ=255)
                                     atatGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCg                                                                                                             >  1:1128095/1‑150 (MQ=255)
                                       atGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGAt                                                                                                           >  2:4157517/1‑150 (MQ=255)
                                         gAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATcc                                                                                                         <  2:271035/150‑1 (MQ=255)
                                         gAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATcc                                                                                                         <  2:4932248/150‑1 (MQ=255)
                                             ggcggGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGcc                                                                                                     <  2:1898337/150‑1 (MQ=255)
                                               cggGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCCATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCaa                                                                                                   >  2:2924905/1‑150 (MQ=255)
                                                   ttAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGt                                                                                               >  1:5253999/1‑150 (MQ=255)
                                                     aaaaTATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTAc                                                                                             >  2:4056327/1‑150 (MQ=255)
                                                          atTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGcc                                                                                        >  1:449933/1‑150 (MQ=255)
                                                             tACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCAtt                                                                                     <  2:4387756/150‑1 (MQ=255)
                                                             tACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCAtt                                                                                     <  2:1719515/150‑1 (MQ=255)
                                                               cAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGc                                                                                   <  2:115040/150‑1 (MQ=255)
                                                               cAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGc                                                                                   <  2:2302717/150‑1 (MQ=255)
                                                                aaCTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCg                                                                                  >  2:5179158/1‑150 (MQ=255)
                                                                  cTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGaa                                                                                <  1:192560/150‑1 (MQ=255)
                                                                   ttAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGaaa                                                                               >  2:4619667/1‑150 (MQ=255)
                                                                     aGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAAt                                                                             >  2:3741851/1‑150 (MQ=255)
                                                                        aaTCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGAc                                                                          >  1:4899084/1‑150 (MQ=255)
                                                                        aaTCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGAc                                                                          >  2:3865425/1‑150 (MQ=255)
                                                                           cAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGt                                                                       <  2:4812718/150‑1 (MQ=255)
                                                                             aCCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGa                                                                     <  1:1262562/150‑1 (MQ=255)
                                                                              ccATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGaa                                                                    <  2:2227810/150‑1 (MQ=255)
                                                                               cATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAAc                                                                   >  1:2531708/1‑150 (MQ=255)
                                                                                aTTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACa                                                                  <  2:2074908/150‑1 (MQ=255)
                                                                                 ttAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACAt                                                                 >  2:292237/1‑150 (MQ=255)
                                                                                 ttAACGCTTGATATCGCTTTTAAAGTCGCGTTATTCATATCCAGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCTCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGACCAt                                                                 >  2:1056459/1‑150 (MQ=255)
                                                                                  tAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATg                                                                <  2:5040836/150‑1 (MQ=255)
                                                                                     cGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGaa                                                             <  1:2730608/150‑1 (MQ=255)
                                                                                      gCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAg                                                            >  1:3276579/1‑150 (MQ=255)
                                                                                       cTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGa                                                           >  1:2630711/1‑150 (MQ=255)
                                                                                          gATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACgg                                                        >  2:3255629/1‑150 (MQ=255)
                                                                                           atatCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGa                                                       >  1:4859667/1‑150 (MQ=255)
                                                                                           atatCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGa                                                       >  2:4243648/1‑150 (MQ=255)
                                                                                             atCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAAt                                                     >  2:3826532/1‑150 (MQ=255)
                                                                                              tCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATa                                                    <  2:4670671/150‑1 (MQ=255)
                                                                                              tCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATa                                                    <  2:877318/150‑1 (MQ=255)
                                                                                              tCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATa                                                    >  1:1095869/1‑150 (MQ=255)
                                                                                                gCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATAcc                                                  <  1:2213646/150‑1 (MQ=255)
                                                                                                 cTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGAAGGAATAccc                                                 >  1:2061330/1‑150 (MQ=255)
                                                                                                   tttAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCAt                                               <  2:4354293/150‑1 (MQ=255)
                                                                                                    ttAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATc                                              >  2:741228/1‑150 (MQ=255)
                                                                                                     tAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCg                                             <  1:1252488/150‑1 (MQ=255)
                                                                                                       aaGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCt                                           >  1:517094/1‑150 (MQ=255)
                                                                                                        aGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCtt                                          >  1:3554724/1‑150 (MQ=255)
                                                                                                          tCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTc                                        <  1:4583657/150‑1 (MQ=255)
                                                                                                               tttttCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGgat                                   <  1:4339859/150‑1 (MQ=255)
                                                                                                                 tttCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGACTACCCATCGCTTTCAGgatga                                 >  2:4906368/1‑150 (MQ=255)
                                                                                                                 tttCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGgatga                                 >  2:4885455/1‑150 (MQ=255)
                                                                                                                   tCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATa                               <  1:3520281/150‑1 (MQ=255)
                                                                                                                    cATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATAc                              >  1:1704726/1‑150 (MQ=255)
                                                                                                                    cATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATAc                              >  2:1505135/1‑150 (MQ=255)
                                                                                                                    cATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATAc                              >  2:2807089/1‑150 (MQ=255)
                                                                                                                     atatCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATAcc                             >  2:363659/1‑150 (MQ=255)
                                                                                                                     atatCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATAcc                             >  2:2429382/1‑150 (MQ=255)
                                                                                                                     atatCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATAcc                             >  2:1404132/1‑150 (MQ=255)
                                                                                                                         ccTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCTCTTTCAGGATGATACCagag                         >  1:284407/1‑150 (MQ=255)
                                                                                                                          cTGTATACAGCTGTCGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGt                        <  1:3965993/150‑1 (MQ=255)
                                                                                                                          cTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGt                        >  2:809252/1‑150 (MQ=255)
                                                                                                                           tGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTa                       <  2:3388790/150‑1 (MQ=255)
                                                                                                                           tGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTa                       <  2:5225296/150‑1 (MQ=255)
                                                                                                                            gTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAg                      >  2:2591391/1‑150 (MQ=255)
                                                                                                                            gTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAg                      >  1:4136119/1‑150 (MQ=255)
                                                                                                                             tataCAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGa                     >  2:684117/1‑150 (MQ=255)
                                                                                                                                 cAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATc                 >  1:3108856/1‑150 (MQ=255)
                                                                                                                                 cAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATc                 <  2:4467451/150‑1 (MQ=255)
                                                                                                                                 cAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATc                 >  2:408929/1‑150 (MQ=255)
                                                                                                                                  aGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCg                >  2:2770827/1‑150 (MQ=255)
                                                                                                                                  aGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCg                >  1:2297069/1‑150 (MQ=255)
                                                                                                                                   gCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGa               >  2:2501811/1‑150 (MQ=255)
                                                                                                                                    cTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGAc              <  1:1312296/150‑1 (MQ=255)
                                                                                                                                       aCGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGtt           <  2:3704748/150‑1 (MQ=255)
                                                                                                                                         gcgGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCg         >  1:762437/1‑150 (MQ=255)
                                                                                                                                             aCGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTa     <  1:1505416/150‑1 (MQ=255)
                                                                                                                                              cGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTAc    <  1:2356504/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAG  >  CP009273/748560‑748853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTT  >  CP009273/748558‑748856
                                                                                                                                                     |                                                                                                                                                     
ACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCT                                                                                                                                                       <  SRR19973062.4388037/150‑1 (MQ=60)
 CAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTA                                                                                                                                                      >  SRR19973062.4691023/1‑150 (MQ=60)
 CAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTA                                                                                                                                                      >  SRR19973062.4691032/1‑150 (MQ=60)
  AATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTAT                                                                                                                                                     >  SRR19973062.2085525/1‑150 (MQ=60)
  AATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTAT                                                                                                                                                     <  SRR19973062.4736097/150‑1 (MQ=60)
   ATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATC                                                                                                                                                    >  SRR19973062.4372945/1‑150 (MQ=60)
    TTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCT                                                                                                                                                   <  SRR19973062.7961/150‑1 (MQ=60)
    TTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCT                                                                                                                                                   <  SRR19973062.5226194/150‑1 (MQ=60)
    TTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCT                                                                                                                                                   <  SRR19973062.4919012/150‑1 (MQ=60)
     TTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTT                                                                                                                                                  <  SRR19973062.797884/150‑1 (MQ=60)
      TTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTC                                                                                                                                                 >  SRR19973062.3723765/1‑150 (MQ=60)
       TTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCA                                                                                                                                                <  SRR19973062.2239846/150‑1 (MQ=60)
       TTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCA                                                                                                                                                <  SRR19973062.2239884/150‑1 (MQ=60)
        TTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCAGACGGGCTATCTTCAT                                                                                                                                               <  SRR19973062.3254984/150‑1 (MQ=60)
         TGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATA                                                                                                                                              <  SRR19973062.2723250/150‑1 (MQ=60)
         TGTGTTTAGGAATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATA                                                                                                                                              <  SRR19973062.3303852/150‑1 (MQ=60)
          GTGTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATAC                                                                                                                                             >  SRR19973062.1464578/1‑150 (MQ=60)
           TGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACC                                                                                                                                            >  SRR19973062.2368482/1‑150 (MQ=60)
            GTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCG                                                                                                                                           <  SRR19973062.5294444/150‑1 (MQ=60)
             TTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGT                                                                                                                                          <  SRR19973062.798181/150‑1 (MQ=60)
               TAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCA                                                                                                                                        <  SRR19973062.3855011/150‑1 (MQ=60)
                AGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCAC                                                                                                                                       >  SRR19973062.3510339/1‑150 (MQ=60)
                AGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCAC                                                                                                                                       >  SRR19973062.3510262/1‑150 (MQ=60)
                AGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCAC                                                                                                                                       >  SRR19973062.3510204/1‑150 (MQ=60)
                 GGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACT                                                                                                                                      <  SRR19973062.5114299/150‑1 (MQ=60)
                  GCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTG                                                                                                                                     >  SRR19973062.4913347/1‑150 (MQ=60)
                   CATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGT                                                                                                                                    <  SRR19973062.1674931/150‑1 (MQ=60)
                   CATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGT                                                                                                                                    <  SRR19973062.1674939/150‑1 (MQ=60)
                    ATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTG                                                                                                                                   >  SRR19973062.2230126/1‑150 (MQ=60)
                    ATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTG                                                                                                                                   >  SRR19973062.2522429/1‑150 (MQ=60)
                    ATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTG                                                                                                                                   >  SRR19973062.1051315/1‑150 (MQ=60)
                      ATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCA                                                                                                                                 >  SRR19973062.34864/1‑150 (MQ=60)
                       TAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCAT                                                                                                                                >  SRR19973062.1864829/1‑150 (MQ=60)
                          AAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTC                                                                                                                             <  SRR19973062.1293507/150‑1 (MQ=60)
                          AAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTC                                                                                                                             <  SRR19973062.1294150/150‑1 (MQ=60)
                           AATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCG                                                                                                                            >  SRR19973062.2409221/1‑150 (MQ=60)
                           AATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCG                                                                                                                            >  SRR19973062.2409216/1‑150 (MQ=60)
                             TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCT                                                                                                                          <  SRR19973062.1379872/150‑1 (MQ=60)
                             TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCT                                                                                                                          <  SRR19973062.1379507/150‑1 (MQ=60)
                                ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCA                                                                                                                       <  SRR19973062.1583610/150‑1 (MQ=60)
                                   CGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTG                                                                                                                    <  SRR19973062.1748482/150‑1 (MQ=60)
                                    GCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGG                                                                                                                   >  SRR19973062.3485497/1‑150 (MQ=60)
                                    GCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGG                                                                                                                   >  SRR19973062.830871/1‑150 (MQ=60)
                                     CCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGG                                                                                                                  >  SRR19973062.1098209/1‑150 (MQ=60)
                                       ATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCG                                                                                                                >  SRR19973062.1133381/1‑150 (MQ=60)
                                         ATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGAT                                                                                                              <  SRR19973062.2567731/150‑1 (MQ=60)
                                          TGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATC                                                                                                             >  SRR19973062.1538782/1‑150 (MQ=60)
                                           GAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCC                                                                                                            >  SRR19973062.5134193/1‑150 (MQ=60)
                                           GAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATAAAGCTGACTCGGACGGGCTATCATCATACCGTCACTGTGCATTTAGCTCCAGTGGGCGATCC                                                                                                            >  SRR19973062.1600468/1‑150 (MQ=60)
                                                  GGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAAC                                                                                                     >  SRR19973062.3870621/1‑150 (MQ=60)
                                                  GGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAAC                                                                                                     >  SRR19973062.2593421/1‑150 (MQ=60)
                                                     TTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGT                                                                                                  >  SRR19973062.1537320/1‑150 (MQ=60)
                                                     TTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGT                                                                                                  >  SRR19973062.5281665/1‑150 (MQ=60)
                                                       AAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTAC                                                                                                >  SRR19973062.4040581/1‑150 (MQ=60)
                                                          ATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCCTTTCGCTCCAGTGGGCGATCCAGCCAACGGGACGTG                                                                                             >  SRR19973062.650082/1‑150 (MQ=60)
                                                          ATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTG                                                                                             <  SRR19973062.4586561/150‑1 (MQ=60)
                                                            ATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCC                                                                                           >  SRR19973062.451865/1‑150 (MQ=60)
                                                                    CTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAA                                                                                   <  SRR19973062.193608/150‑1 (MQ=60)
                                                                       AGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCCTGGCGAAAAT                                                                                >  SRR19973062.3242474/1‑150 (MQ=60)
                                                                       AGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAAT                                                                                <  SRR19973062.2837854/150‑1 (MQ=60)
                                                                         CAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGA                                                                              >  SRR19973062.4595989/1‑150 (MQ=60)
                                                                          AATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGAC                                                                             >  SRR19973062.1164900/1‑150 (MQ=60)
                                                                          AATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGAC                                                                             >  SRR19973062.3487525/1‑150 (MQ=60)
                                                                          AATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCATCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCCACGGTACGTGCCCTTGCGAAAATGAC                                                                             >  SRR19973062.4924296/1‑150 (MQ=60)
                                                                              AACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTG                                                                         >  SRR19973062.1124188/1‑150 (MQ=60)
                                                                               ACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGA                                                                        <  SRR19973062.1268381/150‑1 (MQ=60)
                                                                               ACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGA                                                                        <  SRR19973062.1268287/150‑1 (MQ=60)
                                                                               ACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGA                                                                        >  SRR19973062.3833165/1‑150 (MQ=60)
                                                                               ACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTATCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTAATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGA                                                                        <  SRR19973062.1267979/150‑1 (MQ=60)
                                                                                CCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAA                                                                       <  SRR19973062.1713676/150‑1 (MQ=60)
                                                                                 CATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCAGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATTACGGTGAAC                                                                      >  SRR19973062.2545217/1‑150 (MQ=60)
                                                                                  ATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACA                                                                     <  SRR19973062.5062228/150‑1 (MQ=60)
                                                                                    TAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATG                                                                   <  SRR19973062.4364466/150‑1 (MQ=60)
                                                                                       CGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAA                                                                <  SRR19973062.2744987/150‑1 (MQ=60)
                                                                                       CGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAA                                                                >  SRR19973062.5127898/1‑150 (MQ=60)
                                                                                        GCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAG                                                               <  SRR19973062.3557110/150‑1 (MQ=60)
                                                                                        GCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAG                                                               <  SRR19973062.3557087/150‑1 (MQ=60)
                                                                                        GCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAG                                                               <  SRR19973062.1685313/150‑1 (MQ=60)
                                                                                        GCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAG                                                               <  SRR19973062.2262593/150‑1 (MQ=60)
                                                                                        GCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAG                                                               >  SRR19973062.3293563/1‑150 (MQ=60)
                                                                                         CTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGA                                                              >  SRR19973062.2644569/1‑150 (MQ=60)
                                                                                            GATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGG                                                           <  SRR19973062.4330020/150‑1 (MQ=60)
                                                                                             ATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGA                                                          >  SRR19973062.4884734/1‑150 (MQ=60)
                                                                                              TATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAA                                                         <  SRR19973062.1484257/150‑1 (MQ=60)
                                                                                              TATCGCTGTTACAGTCGCGTTTTTCAGATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATCCCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAA                                                         <  SRR19973062.124798/150‑1 (MQ=60)
                                                                                                TCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATA                                                       >  SRR19973062.1101025/1‑150 (MQ=60)
                                                                                                 CGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATAC                                                      <  SRR19973062.1881538/150‑1 (MQ=60)
                                                                                                 CGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATAC                                                      >  SRR19973062.4330094/1‑150 (MQ=60)
                                                                                                  GCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACC                                                     <  SRR19973062.2224735/150‑1 (MQ=60)
                                                                                                   CTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGAAGGAATACCC                                                    >  SRR19973062.2071877/1‑150 (MQ=60)
                                                                                                   CTTTTAAAGTCGCGTTTTTCATATCCAGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACAAGGAAGACGGAATACCC                                                    >  SRR19973062.2370808/1‑150 (MQ=60)
                                                                                                     TTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCAT                                                  <  SRR19973062.4707122/150‑1 (MQ=60)
                                                                                                       TAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCG                                                <  SRR19973062.1258272/150‑1 (MQ=60)
                                                                                                         AAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCT                                              >  SRR19973062.519522/1‑150 (MQ=60)
                                                                                                         AAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTCCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCT                                              >  SRR19973062.519525/1‑150 (MQ=60)
                                                                                                          AGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTT                                             >  SRR19973062.3572663/1‑150 (MQ=60)
                                                                                                           GTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTT                                            >  SRR19973062.826663/1‑150 (MQ=60)
                                                                                                            TCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTC                                           <  SRR19973062.4980203/150‑1 (MQ=60)
                                                                                                            TCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTC                                           <  SRR19973062.4607632/150‑1 (MQ=60)
                                                                                                              GCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAG                                         <  SRR19973062.750174/150‑1 (MQ=60)
                                                                                                                 TTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGAT                                      <  SRR19973062.4362189/150‑1 (MQ=60)
                                                                                                                  TTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATG                                     <  SRR19973062.4690294/150‑1 (MQ=60)
                                                                                                                    TTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGAT                                   <  SRR19973062.361298/150‑1 (MQ=60)
                                                                                                                    TTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGAT                                   <  SRR19973062.1943514/150‑1 (MQ=60)
                                                                                                                     TCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATA                                  <  SRR19973062.3538104/150‑1 (MQ=60)
                                                                                                                     TCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATA                                  <  SRR19973062.4864320/150‑1 (MQ=60)
                                                                                                                      CATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATAC                                 >  SRR19973062.1713069/1‑150 (MQ=60)
                                                                                                                        TATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCA                               <  SRR19973062.4879697/150‑1 (MQ=60)
                                                                                                                         ATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAG                              >  SRR19973062.5237173/1‑150 (MQ=60)
                                                                                                                           CCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGATGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAG                            <  SRR19973062.338021/150‑1 (MQ=60)
                                                                                                                           CCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCTCTTTCAGGATGATACCAGAG                            >  SRR19973062.285802/1‑150 (MQ=60)
                                                                                                                           CCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAG                            <  SRR19973062.5243465/150‑1 (MQ=60)
                                                                                                                           CCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTACATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAG                            <  SRR19973062.2139816/150‑1 (MQ=60)
                                                                                                                            CTGTATACAGCTGTCGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGT                           <  SRR19973062.3986094/150‑1 (MQ=60)
                                                                                                                              GTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAG                         >  SRR19973062.2338725/1‑150 (MQ=60)
                                                                                                                              GTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAG                         >  SRR19973062.4157543/1‑150 (MQ=60)
                                                                                                                                   CAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATC                    <  SRR19973062.4021031/150‑1 (MQ=60)
                                                                                                                                   CAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATC                    >  SRR19973062.815834/1‑150 (MQ=60)
                                                                                                                                   CAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATC                    <  SRR19973062.2844259/150‑1 (MQ=60)
                                                                                                                                   CAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATC                    >  SRR19973062.3124863/1‑150 (MQ=60)
                                                                                                                                    AGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCG                   >  SRR19973062.2309010/1‑150 (MQ=60)
                                                                                                                                    AGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCG                   >  SRR19973062.3640211/1‑150 (MQ=60)
                                                                                                                                      CTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGAC                 <  SRR19973062.1318300/150‑1 (MQ=60)
                                                                                                                                      CTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGAC                 <  SRR19973062.1318286/150‑1 (MQ=60)
                                                                                                                                      CTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGAC                 <  SRR19973062.306601/150‑1 (MQ=60)
                                                                                                                                       TGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACG                >  SRR19973062.207168/1‑150 (MQ=60)
                                                                                                                                          CGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTC             <  SRR19973062.2255224/150‑1 (MQ=60)
                                                                                                                                           GCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCG            >  SRR19973062.765870/1‑150 (MQ=60)
                                                                                                                                           GCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCG            >  SRR19973062.1124315/1‑150 (MQ=60)
                                                                                                                                            CGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGG           <  SRR19973062.5292789/150‑1 (MQ=60)
                                                                                                                                            CGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGG           <  SRR19973062.5293002/150‑1 (MQ=60)
                                                                                                                                            CGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGG           <  SRR19973062.3121783/150‑1 (MQ=60)
                                                                                                                                            CGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGG           <  SRR19973062.3761049/150‑1 (MQ=60)
                                                                                                                                              GACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGT         <  SRR19973062.1657535/150‑1 (MQ=60)
                                                                                                                                               ACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTA        <  SRR19973062.1512823/150‑1 (MQ=60)
                                                                                                                                                CGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTAC       <  SRR19973062.2369069/150‑1 (MQ=60)
                                                                                                                                                  GGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAG     >  SRR19973062.2479548/1‑150 (MQ=60)
                                                                                                                                                    CTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTT   >  SRR19973062.3451258/1‑150 (MQ=60)
                                                                                                                                                    CTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTT   <  SRR19973062.671752/150‑1 (MQ=60)
                                                                                                                                                     TATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTT  >  SRR19973062.163370/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
ACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTT  >  CP009273/748558‑748856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑