Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,181,504 G→T 100% D69Y (GAT→TAT)  pepT → peptidase T

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,181,5040GT99.3% 927.7 / ‑2.6 277D69Y (GAT→TAT) pepTpeptidase T
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (144/131);  total (145/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 277 total aligned reads displayed.

GCAGGGGTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTATGTTC  >  CP009273/1181357‑1181650
                                                                                                                                                   |                                                                                                                                                  
gCAGGGGTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTat                                                                                                                                                  >  1:2744286/1‑150 (MQ=255)
gCAGGGGTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTat                                                                                                                                                  >  1:1892737/1‑150 (MQ=255)
        gagaCAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGc                                                                                                                                          >  2:1396436/1‑150 (MQ=255)
        gagaCAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGc                                                                                                                                          <  1:229543/150‑1 (MQ=255)
         agaCAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCg                                                                                                                                         <  1:1065499/150‑1 (MQ=255)
           aCAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGAt                                                                                                                                       >  2:132121/1‑150 (MQ=255)
            cAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGAtt                                                                                                                                      <  2:1547161/150‑1 (MQ=255)
            cAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGAtt                                                                                                                                      <  2:289625/150‑1 (MQ=255)
               gTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGc                                                                                                                                   >  2:4118103/1‑150 (MQ=255)
                    cAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTAt                                                                                                                              >  1:665357/1‑150 (MQ=255)
                    cAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTAt                                                                                                                              <  2:4794213/150‑1 (MQ=255)
                       cACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTtc                                                                                                                           >  1:290980/1‑150 (MQ=255)
                         cGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTtctc                                                                                                                         >  2:3914256/1‑150 (MQ=255)
                         cGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTtctc                                                                                                                         <  2:4665891/150‑1 (MQ=255)
                         cGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTtctc                                                                                                                         >  2:2398805/1‑150 (MQ=255)
                          ggAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCa                                                                                                                        >  2:2863388/1‑150 (MQ=255)
                          ggAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCa                                                                                                                        >  1:77131/1‑150 (MQ=255)
                           gAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCAt                                                                                                                       >  2:4854790/1‑150 (MQ=255)
                             aGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCAtgt                                                                                                                     >  2:2609842/1‑150 (MQ=255)
                                ccAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGa                                                                                                                  >  1:3995794/1‑150 (MQ=255)
                                 cAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGAt                                                                                                                 >  1:1171207/1‑150 (MQ=255)
                                   aTGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATAc                                                                                                               >  2:65602/1‑150 (MQ=255)
                                    tGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATAcc                                                                                                              >  2:3696940/1‑150 (MQ=255)
                                         gTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCAcc                                                                                                         >  1:524852/1‑150 (MQ=255)
                                         gTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCAcc                                                                                                         >  2:2153078/1‑150 (MQ=255)
                                         gTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCAcc                                                                                                         <  2:2060811/150‑1 (MQ=255)
                                         gTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCAcc                                                                                                         <  1:1752839/150‑1 (MQ=255)
                                         gTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCAcc                                                                                                         >  2:584488/1‑150 (MQ=255)
                                              tGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGAtt                                                                                                    <  2:4377524/150‑1 (MQ=255)
                                              tGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGAtt                                                                                                    >  1:4771921/1‑150 (MQ=255)
                                               gCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTg                                                                                                   <  2:4740405/150‑1 (MQ=255)
                                                cATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGc                                                                                                  >  2:4392209/1‑150 (MQ=255)
                                                   ctgctgAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGc                                                                                               <  2:4084568/150‑1 (MQ=255)
                                                      ctgATAGAGCAGCTCGAAGAGATGGGGCTTATCTATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCataggc                                                                                            <  2:442011/150‑6 (MQ=255)
                                                      ctgAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGc                                                                                            <  1:2118886/150‑1 (MQ=255)
                                                       tgAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCa                                                                                           <  2:1249698/150‑1 (MQ=255)
                                                       tgAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCa                                                                                           >  1:2429496/1‑150 (MQ=255)
                                                         aaaGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCGGCGGCaaa                                                                                         >  2:5052775/1‑150 (MQ=255)
                                                         aaaGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCaaa                                                                                         >  1:3402974/1‑150 (MQ=255)
                                                         aaaGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCaaa                                                                                         >  2:4514576/1‑150 (MQ=255)
                                                          aaGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCaaaa                                                                                        >  2:3033493/1‑150 (MQ=255)
                                                           agagCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCaaaaa                                                                                       >  2:1498584/1‑150 (MQ=255)
                                                              gcagcTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAAtgt                                                                                    >  2:3262395/1‑150 (MQ=255)
                                                                agcTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGa                                                                                  <  2:3556375/150‑1 (MQ=255)
                                                                 gcTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGaa                                                                                 <  1:503057/150‑1 (MQ=255)
                                                                    cGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATcc                                                                              <  1:298920/150‑1 (MQ=255)
                                                                    cGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATcc                                                                              <  2:3765258/150‑1 (MQ=255)
                                                                      aaGAGATGGGGCTTATCAATGTGCCCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTCTATCCCGGCGAGTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGc                                                                            >  2:4867375/1‑150 (MQ=255)
                                                                       agagATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCa                                                                           >  2:4576192/1‑150 (MQ=255)
                                                                             ggggCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAAttgt                                                                     >  1:3390564/1‑150 (MQ=255)
                                                                              gggCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAAttgtt                                                                    <  1:3070639/150‑1 (MQ=255)
                                                                              gggCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAAttgtt                                                                    <  2:1433606/150‑1 (MQ=255)
                                                                                   tATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGaaaa                                                               >  1:1461914/1‑150 (MQ=255)
                                                                                   tATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGaaaa                                                               >  1:4293267/1‑150 (MQ=255)
                                                                                       aaTGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTAt                                                           >  2:423863/1‑150 (MQ=255)
                                                                                             aCCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGt                                                     >  2:1617793/1‑150 (MQ=255)
                                                                                              ccTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTg                                                    <  2:1741122/150‑1 (MQ=255)
                                                                                                 tAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCg                                                 <  1:4535174/150‑1 (MQ=255)
                                                                                                  aaGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGa                                                >  2:3840529/1‑150 (MQ=255)
                                                                                                  aaGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGa                                                <  1:353070/150‑1 (MQ=255)
                                                                                                  aaGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGa                                                >  2:3618444/1‑150 (MQ=255)
                                                                                                    gTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGata                                              <  2:1201535/150‑1 (MQ=255)
                                                                                                     tGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGAGTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGatat                                             >  2:3432203/1‑150 (MQ=255)
                                                                                                      gagaAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATAtt                                            >  2:268239/1‑150 (MQ=255)
                                                                                                        gaAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTgc                                          >  2:3859093/1‑150 (MQ=255)
                                                                                                        gaAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTgc                                          >  1:2706137/1‑150 (MQ=255)
                                                                                                         aaGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTgcg                                         <  1:132121/150‑1 (MQ=255)
                                                                                                           gggCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCt                                       >  2:381301/1‑150 (MQ=255)
                                                                                                           gggCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCt                                       >  2:2777256/1‑150 (MQ=255)
                                                                                                            ggCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTg                                      <  1:1709370/150‑1 (MQ=255)
                                                                                                             gCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTgg                                     <  1:3348986/150‑1 (MQ=255)
                                                                                                              cACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTCTCGCGGTGGCGATATTGCGCTggg                                    <  1:3852663/150‑1 (MQ=255)
                                                                                                              cACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTggg                                    <  2:3478200/150‑1 (MQ=255)
                                                                                                              cACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTggg                                    <  2:5105196/150‑1 (MQ=255)
                                                                                                               aCTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGt                                   <  1:1396436/150‑1 (MQ=255)
                                                                                                                cTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTa                                  >  1:1985451/1‑150 (MQ=255)
                                                                                                                cTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTa                                  <  1:5023092/150‑1 (MQ=255)
                                                                                                                cTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTa                                  <  1:960907/150‑1 (MQ=255)
                                                                                                                 tttGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTAt                                 <  1:4165631/150‑1 (MQ=255)
                                                                                                                   tGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATNg                               <  1:1613746/150‑1 (MQ=255)
                                                                                                                    gATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCgg                              >  1:2598719/1‑150 (MQ=255)
                                                                                                                       ggCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGa                           <  2:861268/150‑1 (MQ=255)
                                                                                                                       ggCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGa                           >  2:1800833/1‑150 (MQ=255)
                                                                                                                        gCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGAt                          >  1:673236/1‑150 (MQ=255)
                                                                                                                        gCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGAt                          >  1:1634298/1‑150 (MQ=255)
                                                                                                                           aCGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGaa                       <  2:3724262/150‑1 (MQ=255)
                                                                                                                           aCGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGaa                       >  1:3337194/1‑150 (MQ=255)
                                                                                                                           aCGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGaa                       >  2:2459934/1‑150 (MQ=255)
                                                                                                                             gTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGt                     >  1:3123457/1‑150 (MQ=255)
                                                                                                                             gTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGt                     >  1:462256/1‑150 (MQ=255)
                                                                                                                                aCCGTCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGtttt                  <  1:4750382/150‑1 (MQ=255)
                                                                                                                                 ccGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTa                 >  1:2186722/1‑150 (MQ=255)
                                                                                                                                 ccGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTa                 <  2:3159583/150‑1 (MQ=255)
                                                                                                                                  cGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTAt                >  2:2946394/1‑150 (MQ=255)
                                                                                                                                      tAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCAcc            >  1:4253240/1‑150 (MQ=255)
                                                                                                                                         cGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGt         <  1:3655158/150‑1 (MQ=255)
                                                                                                                                           tCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTa       <  1:1194011/150‑1 (MQ=255)
                                                                                                                                           tCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTa       <  2:1639650/150‑1 (MQ=255)
                                                                                                                                            cccTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTAt      >  1:1000295/1‑150 (MQ=255)
                                                                                                                                               tGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAATTTTTATCACCGGTTAtttt   >  1:4413111/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCAGGGGTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTATGTTC  >  CP009273/1181357‑1181650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTATGTTCC  >  CP009273/1181356‑1181651
                                                                                                                                                    |                                                                                                                                                   
AGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTA                                                                                                                                                    <  SRR19973063.2172446/150‑1 (MQ=60)
AGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTA                                                                                                                                                    >  SRR19973063.151345/1‑150 (MQ=60)
AGCAGGGGTGAGACAGCGTCCCAGCACGGAAGGCCAATGGACGTTAGTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTA                                                                                                                                                    <  SRR19973063.3035592/150‑1 (MQ=60)
 GCAGGGGTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTAT                                                                                                                                                   >  SRR19973063.1898656/1‑150 (MQ=60)
 GCAGGGGTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTAT                                                                                                                                                   <  SRR19973063.667496/150‑1 (MQ=60)
 GCAGGGGTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTAT                                                                                                                                                   >  SRR19973063.2753142/1‑150 (MQ=60)
         GAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGC                                                                                                                                           <  SRR19973063.230232/150‑1 (MQ=60)
          AGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCG                                                                                                                                          <  SRR19973063.1068390/150‑1 (MQ=60)
          AGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCG                                                                                                                                          <  SRR19973063.1068733/150‑1 (MQ=60)
            ACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGAT                                                                                                                                        >  SRR19973063.4785218/1‑150 (MQ=60)
               GGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGG                                                                                                                                     <  SRR19973063.1187259/150‑1 (MQ=60)
                     CAGCACTGAAGGCCAATTGAAGTTATTGCATCTTCTGTAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAATGGCACTTTTATGGCGACGTTACCGGCTAACGTCCATGGCTATATCCCGGCGATTGTCTTTAT                                                                                                                               <  SRR19973063.3390059/150‑1 (MQ=60)
                     CAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTAT                                                                                                                               >  SRR19973063.667300/1‑150 (MQ=60)
                      AGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTAACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATT                                                                                                                              >  SRR19973063.4306434/1‑150 (MQ=60)
                        CACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTC                                                                                                                            >  SRR19973063.291837/1‑150 (MQ=60)
                         ACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATACCGGCGATTGGCTTTATTTCT                                                                                                                           >  SRR19973063.311025/1‑150 (MQ=60)
                           GGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCA                                                                                                                         <  SRR19973063.1732026/150‑1 (MQ=60)
                           GGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCA                                                                                                                         >  SRR19973063.77401/1‑150 (MQ=60)
                           GGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCA                                                                                                                         <  SRR19973063.744275/150‑1 (MQ=60)
                             AAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATG                                                                                                                       <  SRR19973063.4768937/150‑1 (MQ=60)
                             AAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATG                                                                                                                       >  SRR19973063.2161018/1‑150 (MQ=60)
                             AAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATG                                                                                                                       >  SRR19973063.1638663/1‑150 (MQ=60)
                             AAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATG                                                                                                                       <  SRR19973063.17221/150‑1 (MQ=60)
                              AGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGT                                                                                                                      <  SRR19973063.2997248/150‑1 (MQ=60)
                              AGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGT                                                                                                                      <  SRR19973063.526350/150‑1 (MQ=60)
                                 CCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGA                                                                                                                   >  SRR19973063.4008609/1‑150 (MQ=60)
                                 CCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGA                                                                                                                   >  SRR19973063.1989985/1‑150 (MQ=60)
                                  CAATGGTAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGAT                                                                                                                  >  SRR19973063.2603802/1‑150 (MQ=60)
                                  CAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGAT                                                                                                                  >  SRR19973063.2603782/1‑150 (MQ=60)
                                  CAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGAT                                                                                                                  >  SRR19973063.1174739/1‑150 (MQ=60)
                                       GAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTC                                                                                                             <  SRR19973063.1180820/150‑1 (MQ=60)
                                          GTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACC                                                                                                          <  SRR19973063.1758219/150‑1 (MQ=60)
                                          GTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACC                                                                                                          >  SRR19973063.526390/1‑150 (MQ=60)
                                          GTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACC                                                                                                          >  SRR19973063.1554351/1‑150 (MQ=60)
                                            TATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGG                                                                                                        >  SRR19973063.3317561/1‑150 (MQ=60)
                                             ATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGA                                                                                                       >  SRR19973063.3069636/1‑150 (MQ=60)
                                               TGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATT                                                                                                     >  SRR19973063.4787157/1‑150 (MQ=60)
                                               TGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATT                                                                                                     <  SRR19973063.4409468/150‑1 (MQ=60)
                                               TGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATT                                                                                                     <  SRR19973063.510357/150‑1 (MQ=60)
                                               TGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATT                                                                                                     >  SRR19973063.402698/1‑150 (MQ=60)
                                                 CATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGC                                                                                                   <  SRR19973063.2824744/150‑1 (MQ=60)
                                                  ATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCA                                                                                                  <  SRR19973063.3467181/150‑1 (MQ=60)
                                                  ATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCA                                                                                                  <  SRR19973063.2403737/150‑1 (MQ=60)
                                                       CTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGC                                                                                             <  SRR19973063.2125512/150‑1 (MQ=60)
                                                        TGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTTCAGCGGCA                                                                                            >  SRR19973063.2437259/1‑150 (MQ=60)
                                                        TGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCA                                                                                            <  SRR19973063.4544847/150‑1 (MQ=60)
                                                        TGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCA                                                                                            >  SRR19973063.2436923/1‑150 (MQ=60)
                                                         GAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAA                                                                                           >  SRR19973063.3372124/1‑150 (MQ=60)
                                                          AAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAA                                                                                          >  SRR19973063.3413797/1‑150 (MQ=60)
                                                            AGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAA                                                                                        >  SRR19973063.376271/1‑150 (MQ=60)
                                                            AGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAA                                                                                        >  SRR19973063.340935/1‑150 (MQ=60)
                                                             GAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAAT                                                                                       <  SRR19973063.1975606/150‑1 (MQ=60)
                                                             GAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAAT                                                                                       <  SRR19973063.1336234/150‑1 (MQ=60)
                                                              AGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATG                                                                                      <  SRR19973063.272417/150‑1 (MQ=60)
                                                                  GCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAA                                                                                  <  SRR19973063.504512/150‑1 (MQ=60)
                                                                     CGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCC                                                                               <  SRR19973063.299803/150‑1 (MQ=60)
                                                                      GAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCG                                                                              >  SRR19973063.1444608/1‑150 (MQ=60)
                                                                      GAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCG                                                                              <  SRR19973063.11001/150‑1 (MQ=60)
                                                                       AAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGC                                                                             >  SRR19973063.3354213/1‑150 (MQ=60)
                                                                         GAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAA                                                                           <  SRR19973063.45854/150‑1 (MQ=60)
                                                                         GAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAA                                                                           >  SRR19973063.1518932/1‑150 (MQ=60)
                                                                            ATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATT                                                                        <  SRR19973063.1652656/150‑1 (MQ=60)
                                                                            ATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATT                                                                        >  SRR19973063.141797/1‑150 (MQ=60)
                                                                              GGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGT                                                                      >  SRR19973063.3401362/1‑150 (MQ=60)
                                                                               GGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTT                                                                     <  SRR19973063.3080503/150‑1 (MQ=60)
                                                                                GGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCTGCGGCAAAAATGTGAATCCGCAAATTGTTG                                                                    >  SRR19973063.3317576/1‑150 (MQ=60)
                                                                                GGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTG                                                                    >  SRR19973063.757463/1‑150 (MQ=60)
                                                                                GGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTG                                                                    >  SRR19973063.4547962/1‑150 (MQ=60)
                                                                                GGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACATCAGCGGATTGCAGCGGCAAAACTGTGAATCCGCAAATTGTTG                                                                    >  SRR19973063.3350577/1‑150 (MQ=60)
                                                                                    TATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAA                                                                >  SRR19973063.4306908/1‑150 (MQ=60)
                                                                                    TATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAA                                                                >  SRR19973063.1466375/1‑150 (MQ=60)
                                                                                         ATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCGTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATC                                                           >  SRR19973063.636051/1‑150 (MQ=60)
                                                                                          TGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCG                                                          <  SRR19973063.1862611/150‑1 (MQ=60)
                                                                                               CCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTG                                                     <  SRR19973063.2196800/150‑1 (MQ=60)
                                                                                               CCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTG                                                     <  SRR19973063.373236/150‑1 (MQ=60)
                                                                                                  TAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCG                                                  <  SRR19973063.4549715/150‑1 (MQ=60)
                                                                                                  TAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCG                                                  <  SRR19973063.4284243/150‑1 (MQ=60)
                                                                                                   AAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGA                                                 <  SRR19973063.354084/150‑1 (MQ=60)
                                                                                                    AGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAAATGTTGAAAACTATCTCGGTGGCGAT                                                >  SRR19973063.2880404/1‑150 (MQ=60)
                                                                                                      TGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATAT                                              >  SRR19973063.3892144/1‑150 (MQ=60)
                                                                                                       GAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATT                                             <  SRR19973063.812893/150‑1 (MQ=60)
                                                                                                        AGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTG                                            >  SRR19973063.4551690/1‑150 (MQ=60)
                                                                                                        AGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTG                                            <  SRR19973063.2010944/150‑1 (MQ=60)
                                                                                                        AGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTG                                            >  SRR19973063.504209/1‑150 (MQ=60)
                                                                                                        AGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGAGATTG                                            >  SRR19973063.504032/1‑150 (MQ=60)
                                                                                                         GAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGC                                           >  SRR19973063.2714874/1‑150 (MQ=60)
                                                                                                         GAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGC                                           <  SRR19973063.4752734/150‑1 (MQ=60)
                                                                                                          AAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCG                                          >  SRR19973063.3639414/1‑150 (MQ=60)
                                                                                                          AAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCG                                          <  SRR19973063.132541/150‑1 (MQ=60)
                                                                                                             GGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTG                                       <  SRR19973063.1714575/150‑1 (MQ=60)
                                                                                                              GCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGG                                      <  SRR19973063.3359653/150‑1 (MQ=60)
                                                                                                              GCACTTTGATGGCGACGTGACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGG                                      <  SRR19973063.4234055/150‑1 (MQ=60)
                                                                                                               CACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTCTCGCGGTGGCGATATTGCGCTGGG                                     <  SRR19973063.3864912/150‑1 (MQ=60)
                                                                                                                ACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGT                                    <  SRR19973063.1400658/150‑1 (MQ=60)
                                                                                                                 CTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTA                                   <  SRR19973063.963799/150‑1 (MQ=60)
                                                                                                                 CTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTA                                   <  SRR19973063.5039295/150‑1 (MQ=60)
                                                                                                                 CTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTA                                   >  SRR19973063.1991655/1‑150 (MQ=60)
                                                                                                                  TTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTAT                                  <  SRR19973063.4178953/150‑1 (MQ=60)
                                                                                                                  TTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTAT                                  >  SRR19973063.4150248/1‑150 (MQ=60)
                                                                                                                   TTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATC                                 >  SRR19973063.535707/1‑150 (MQ=60)
                                                                                                                   TTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATC                                 <  SRR19973063.2631026/150‑1 (MQ=60)
                                                                                                                    TGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATNG                                <  SRR19973063.1618683/150‑1 (MQ=60)
                                                                                                                    TGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCG                                >  SRR19973063.935182/1‑150 (MQ=60)
                                                                                                                    TGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCG                                <  SRR19973063.1618874/150‑1 (MQ=60)
                                                                                                                     GATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGG                               >  SRR19973063.2606980/1‑150 (MQ=60)
                                                                                                                      ATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGC                              >  SRR19973063.2016359/1‑150 (MQ=60)
                                                                                                                        GGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGA                            <  SRR19973063.1287902/150‑1 (MQ=60)
                                                                                                                         GCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGAT                           >  SRR19973063.4224667/1‑150 (MQ=60)
                                                                                                                         GCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGAT                           >  SRR19973063.675205/1‑150 (MQ=60)
                                                                                                                         GCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGAT                           >  SRR19973063.1639293/1‑150 (MQ=60)
                                                                                                                          CGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATG                          >  SRR19973063.108764/1‑150 (MQ=60)
                                                                                                                           GACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGA                         >  SRR19973063.4970125/1‑150 (MQ=60)
                                                                                                                            ACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAA                        >  SRR19973063.3347834/1‑150 (MQ=60)
                                                                                                                              GTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGT                      >  SRR19973063.292293/1‑150 (MQ=60)
                                                                                                                              GTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGT                      >  SRR19973063.3133514/1‑150 (MQ=60)
                                                                                                                              GTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGT                      >  SRR19973063.463560/1‑150 (MQ=60)
                                                                                                                              GTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGT                      >  SRR19973063.463630/1‑150 (MQ=60)
                                                                                                                              GTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGT                      >  SRR19973063.3133502/1‑150 (MQ=60)
                                                                                                                                 ACCGTCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTT                   <  SRR19973063.4765519/150‑1 (MQ=60)
                                                                                                                                 ACCGGCTAACGTCCCTGGCTATATACCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTT                   <  SRR19973063.1216537/150‑1 (MQ=60)
                                                                                                                                  CCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTA                  >  SRR19973063.2193577/1‑150 (MQ=60)
                                                                                                                                     GCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCA               <  SRR19973063.3474990/150‑1 (MQ=60)
                                                                                                                                       TAACGTCCCTGGCTATCTCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACC             <  SRR19973063.2280779/150‑1 (MQ=60)
                                                                                                                                       TAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACC             >  SRR19973063.4266785/1‑150 (MQ=60)
                                                                                                                                        AACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCG            >  SRR19973063.2734817/1‑150 (MQ=60)
                                                                                                                                          CGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGT          <  SRR19973063.3666878/150‑1 (MQ=60)
                                                                                                                                          CGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGT          <  SRR19973063.3666880/150‑1 (MQ=60)
                                                                                                                                            TCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTA        <  SRR19973063.1197595/150‑1 (MQ=60)
                                                                                                                                             CCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTAT       >  SRR19973063.1003311/1‑150 (MQ=60)
                                                                                                                                               CTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTATGT     >  SRR19973063.188073/1‑150 (MQ=60)
                                                                                                                                                TGGCTCTATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTATGTT    >  SRR19973063.3851694/1‑150 (MQ=60)
                                                                                                                                                TGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAATTTTTATCACCGGTTATTTT    >  SRR19973063.4427268/1‑150 (MQ=60)
                                                                                                                                                  GCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTATGTTCC  <  SRR19973063.1510712/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
AGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTATGTTCC  >  CP009273/1181356‑1181651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑