Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F488 I0 R1	1275	300.1	10328444	91.0%	9398884	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,570,826	C→A	100%	D351Y (GAC→TAC)	yddB ←	putative TonB‑dependent outer membrane receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,570,826	0	C	A	98.7%	779.1 / ‑0.1	239	D351Y (GAC→TAC)	yddB	putative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand): ref base C (1/2); new base A (126/109); total (128/111)
Fisher's exact test for biased strand distribution p-value = 6.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 239 total aligned reads displayed.

AAACATTACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTCATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCGGGAAGGTGCTGGTAT  >  CP009273/1570679‑1570972

 aaCATTACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATg                                                                                                                                                 >  1:122426/1‑150 (MQ=255)

 aaCATTACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATg                                                                                                                                                 >  2:1786791/1‑150 (MQ=255)

  aCATTACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGa                                                                                                                                                <  2:3922392/150‑1 (MQ=255)

   cATTACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGAt                                                                                                                                               >  1:1295601/1‑150 (MQ=255)

    aTTACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATc                                                                                                                                              <  1:4903918/150‑1 (MQ=255)

      tACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGt                                                                                                                                            <  2:1727879/150‑1 (MQ=255)

       aCCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTg                                                                                                                                           >  1:2402288/1‑150 (MQ=255)

       aCCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTg                                                                                                                                           <  1:1565879/150‑1 (MQ=255)

        cccACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGa                                                                                                                                          >  2:922127/1‑150 (MQ=255)

         ccACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGAc                                                                                                                                         >  2:1238838/1‑150 (MQ=255)

         ccACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGAc                                                                                                                                         >  2:64951/1‑150 (MQ=255)

          cACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACg                                                                                                                                        <  2:1466775/150‑1 (MQ=255)

          cACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACg                                                                                                                                        <  2:3383175/150‑1 (MQ=255)

            cGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACggg                                                                                                                                      >  2:1142664/1‑150 (MQ=255)

            cGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACggg                                                                                                                                      <  2:3922167/150‑1 (MQ=255)

            cGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACggg                                                                                                                                      <  2:4045605/150‑1 (MQ=255)

                 aaTTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATaa                                                                                                                                 <  2:4820966/150‑1 (MQ=255)

                    ttttGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCa                                                                                                                              <  2:783740/150‑1 (MQ=255)

                      ttGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACt                                                                                                                            >  2:3680463/1‑150 (MQ=255)

                      ttGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACt                                                                                                                            <  2:4238443/150‑1 (MQ=255)

                         ccAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAat                                                                                                                         <  2:1380862/150‑1 (MQ=255)

                               cAGGCGTGTTTTGAAGGTGTACTTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATc                                                                                                                   <  2:2703228/150‑1 (MQ=255)

                               cAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATc                                                                                                                   <  2:2700394/150‑1 (MQ=255)

                               cAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATc                                                                                                                   >  1:3168551/1‑150 (MQ=255)

                               cAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATc                                                                                                                   >  1:3355121/1‑150 (MQ=255)

                               cAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATc                                                                                                                   >  1:2482459/1‑150 (MQ=255)

                                aGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATcc                                                                                                                  <  1:601160/150‑1 (MQ=255)

                                aGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATcc                                                                                                                  <  2:1054390/150‑1 (MQ=255)

                                aGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATcc                                                                                                                  <  1:3741141/150‑1 (MQ=255)

                                aGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATcc                                                                                                                  >  1:1821987/1‑150 (MQ=255)

                                 ggCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATccc                                                                                                                 <  1:2818054/150‑1 (MQ=255)

                                    gtgtTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCccaac                                                                                                              <  1:1375097/150‑1 (MQ=255)

                                    gtgtTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCccaac                                                                                                              <  2:2854276/150‑1 (MQ=255)

                                      gtTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCccaacca                                                                                                            <  1:408490/150‑1 (MQ=255)

                                        tttGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCcaaccaac                                                                                                          <  2:3299383/150‑1 (MQ=255)

                                          tGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACgg                                                                                                        >  2:2036470/1‑150 (MQ=255)

                                            aaGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTCATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACggtg                                                                                                      >  1:3570342/1‑150 (MQ=255)

                                             aGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACggtgg                                                                                                     >  1:4062054/1‑150 (MQ=255)

                                              ggTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACggtggt                                                                                                    <  1:2421527/150‑1 (MQ=255)

                                                tgtAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTAc                                                                                                  <  1:902753/150‑1 (MQ=255)

                                                   aaTTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCa                                                                                               >  1:4322850/1‑150 (MQ=255)

                                                     ttATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGt                                                                                             <  1:4506019/150‑1 (MQ=255)

                                                        tCTACAGCCGGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTg                                                                                          <  2:4702439/150‑1 (MQ=255)

                                                          tACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGc                                                                                        >  1:3120946/1‑150 (MQ=255)

                                                            cAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGccc                                                                                      >  2:273201/1‑150 (MQ=255)

                                                            cAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGccc                                                                                      <  1:3477126/150‑1 (MQ=255)

                                                             aGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCa                                                                                     <  2:3693225/150‑1 (MQ=255)

                                                             aGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCa                                                                                     >  2:2161989/1‑150 (MQ=255)

                                                                cTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGc                                                                                  >  1:2851714/1‑150 (MQ=255)

                                                                  gggAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGa                                                                                <  2:4449188/150‑1 (MQ=255)

                                                                  gggAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGa                                                                                >  2:1679509/1‑150 (MQ=255)

                                                                    gAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGc                                                                              <  2:3087446/150‑1 (MQ=255)

                                                                      aaTGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGATGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTg                                                                            >  1:3885845/1‑150 (MQ=255)

                                                                      aaTGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTg                                                                            >  1:4941488/1‑150 (MQ=255)

                                                                      aaTGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTg                                                                            <  1:4249226/150‑1 (MQ=255)

                                                                       aTGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGc                                                                           <  2:4733668/150‑1 (MQ=255)

                                                                             ccTAATCCGCCTCGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATcc                                                                     <  1:4155823/150‑1 (MQ=255)

                                                                             ccTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATcc                                                                     <  2:3168551/150‑1 (MQ=255)

                                                                             ccTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATcc                                                                     <  2:3626540/150‑1 (MQ=255)

                                                                             ccTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATcc                                                                     <  1:791008/150‑1 (MQ=255)

                                                                               tAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGTCCCATGCGAGCTGCGTATCCat                                                                   >  2:23977/1‑150 (MQ=255)

                                                                               tAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCat                                                                   >  1:150013/1‑150 (MQ=255)

                                                                               tAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCat                                                                   >  1:4438466/1‑150 (MQ=255)

                                                                                aaTCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCata                                                                  >  2:2507157/1‑150 (MQ=255)

                                                                                 aTCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCatat                                                                 >  1:2035265/1‑150 (MQ=255)

                                                                                   ccGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATcc                                                               >  1:2550289/1‑150 (MQ=255)

                                                                                     gCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCa                                                             >  2:4075706/1‑150 (MQ=255)

                                                                                      ccACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCAt                                                            >  1:364595/1‑150 (MQ=255)

                                                                                       cACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTC‑‑TGTA‑‑‑‑‑‑‑‑‑ATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAg                                                >  1:4069609/1‑150 (MQ=255)

                                                                                         cGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGcc                                                         <  2:1763265/150‑1 (MQ=255)

                                                                                          gggTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCACCGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCa                                                        <  1:2199706/150‑1 (MQ=255)

                                                                                               cAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCa                                                   >  2:3963211/1‑150 (MQ=255)

                                                                                                  cGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAg                                                <  2:711873/150‑1 (MQ=255)

                                                                                                  cGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAg                                                >  1:2273377/1‑150 (MQ=255)

                                                                                                        gTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATtta                                          <  2:1108206/150‑1 (MQ=255)

                                                                                                          aaTATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATttatt                                        <  2:4975343/150‑1 (MQ=255)

                                                                                                           atatCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATttatta                                       >  1:527161/1‑150 (MQ=255)

                                                                                                           atatCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATttatta                                       >  1:4407598/1‑150 (MQ=255)

                                                                                                                 ccATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCAtt                                 <  1:722565/150‑1 (MQ=255)

                                                                                                                  cATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCAttt                                <  1:299287/150‑1 (MQ=255)

                                                                                                                   atatGTACATTAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTc                               <  1:3945709/150‑1 (MQ=255)

                                                                                                                       gTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGa                           >  1:1076550/1‑150 (MQ=255)

                                                                                                                       gTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGa                           >  1:1056119/1‑150 (MQ=255)

                                                                                                                       gTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGa                           <  2:2402288/150‑1 (MQ=255)

                                                                                                                        tACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATATCGCGAt                          >  2:2628428/1‑150 (MQ=255)

                                                                                                                           aTGAATGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAg                       <  1:1786791/150‑1 (MQ=255)

                                                                                                                           aTGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAg                       >  1:3288269/1‑150 (MQ=255)

                                                                                                                            tGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGa                      >  1:4318478/1‑150 (MQ=255)

                                                                                                                             gAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGAc                     >  2:3409259/1‑150 (MQ=255)

                                                                                                                              aaaGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACt                    >  1:511222/1‑150 (MQ=255)

                                                                                                                              aaaGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACt                    >  1:4233042/1‑150 (MQ=255)

                                                                                                                                aGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGc                  >  1:4628418/1‑150 (MQ=255)

                                                                                                                                aGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGc                  <  2:1504159/150‑1 (MQ=255)

                                                                                                                                  ttCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCgg                >  2:5063328/1‑150 (MQ=255)

                                                                                                                                     ggTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCGGGaa             <  2:3405917/150‑1 (MQ=255)

                                                                                                                                     ggTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATAAGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGACAGACCATAGGATTTATTACCCATTTCGCGATCAGACAGCgataa             >  1:4753095/1‑146 (MQ=255)

                                                                                                                                         tACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCGGGAAGGTg         <  1:1451771/150‑1 (MQ=255)

                                                                                                                                           ccAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCGGGAAGGTGCt       >  2:2807094/1‑150 (MQ=255)

                                                                                                                                             aGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCGGGAAGGTGCTgg     >  1:2624492/1‑150 (MQ=255)

                                                                                                                                                tGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCGGGAAGGTGCTGGtat  >  2:887029/1‑150 (MQ=255)

AAACATTACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTCATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCGGGAAGGTGCTGGTAT  >  CP009273/1570679‑1570972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGAAACATTACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTCATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCGGGAAGGTGCTGGTATTAT  >  CP009273/1570677‑1570975

CGAAACATTACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTA                                                                                                                                                       >  SRR19973063.393671/1‑150 (MQ=60)

CGAAACATTACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTA                                                                                                                                                       >  SRR19973063.393670/1‑150 (MQ=60)

CGAAACATTACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTA                                                                                                                                                       <  SRR19973063.3282035/150‑1 (MQ=60)

 GAAACATTACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAA                                                                                                                                                      >  SRR19973063.3794227/1‑150 (MQ=60)

   AACATTACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATG                                                                                                                                                    <  SRR19973063.2309865/150‑1 (MQ=60)

   AACATTACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATG                                                                                                                                                    >  SRR19973063.122827/1‑150 (MQ=60)

   AACATTACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATG                                                                                                                                                    <  SRR19973063.3350907/150‑1 (MQ=60)

    ACATTACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGA                                                                                                                                                   <  SRR19973063.4099235/150‑1 (MQ=60)

     CATTACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGAT                                                                                                                                                  >  SRR19973063.1299457/1‑150 (MQ=60)

      ATTACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATC                                                                                                                                                 <  SRR19973063.4919751/150‑1 (MQ=60)

         ACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTG                                                                                                                                              >  SRR19973063.2409972/1‑150 (MQ=60)

         ACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTG                                                                                                                                              <  SRR19973063.1570687/150‑1 (MQ=60)

         ACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGAAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTG                                                                                                                                              >  SRR19973063.2809020/1‑150 (MQ=60)

           CCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGAC                                                                                                                                            <  SRR19973063.3820302/150‑1 (MQ=60)

              CGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGG                                                                                                                                         >  SRR19973063.927771/1‑150 (MQ=60)

                  GAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATA                                                                                                                                     >  SRR19973063.2554885/1‑150 (MQ=60)

                        TTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACT                                                                                                                               >  SRR19973063.2146992/1‑150 (MQ=60)

                            CAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATA                                                                                                                           >  SRR19973063.1470070/1‑150 (MQ=60)

                             AGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATAT                                                                                                                          <  SRR19973063.308918/150‑1 (MQ=60)

                              GTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATG                                                                                                                         >  SRR19973063.4440338/1‑150 (MQ=60)

                                 CAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATC                                                                                                                      >  SRR19973063.3365798/1‑150 (MQ=60)

                                 CAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATC                                                                                                                      >  SRR19973063.3638182/1‑150 (MQ=60)

                                 CAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATC                                                                                                                      >  SRR19973063.3178751/1‑150 (MQ=60)

                                 CAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATC                                                                                                                      >  SRR19973063.2490389/1‑150 (MQ=60)

                                  AGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCC                                                                                                                     <  SRR19973063.3753092/150‑1 (MQ=60)

                                  AGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCC                                                                                                                     >  SRR19973063.1827657/1‑150 (MQ=60)

                                  AGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCC                                                                                                                     >  SRR19973063.2184762/1‑150 (MQ=60)

                                  AGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCC                                                                                                                     <  SRR19973063.602927/150‑1 (MQ=60)

                                   GGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCC                                                                                                                    <  SRR19973063.2827113/150‑1 (MQ=60)

                                   GGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCC                                                                                                                    <  SRR19973063.3573093/150‑1 (MQ=60)

                                      GTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAAC                                                                                                                 <  SRR19973063.1379202/150‑1 (MQ=60)

                                      GTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAAC                                                                                                                 <  SRR19973063.1379245/150‑1 (MQ=60)

                                      GTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAAC                                                                                                                 <  SRR19973063.3401723/150‑1 (MQ=60)

                                        GTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCA                                                                                                               <  SRR19973063.409640/150‑1 (MQ=60)

                                           TTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACG                                                                                                            >  SRR19973063.1076780/1‑150 (MQ=60)

                                            TGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGG                                                                                                           <  SRR19973063.1292520/150‑1 (MQ=60)

                                              AAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTCATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTG                                                                                                         >  SRR19973063.3581790/1‑150 (MQ=60)

                                               AGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGG                                                                                                        >  SRR19973063.4077547/1‑150 (MQ=60)

                                               AGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGG                                                                                                        >  SRR19973063.4075073/1‑150 (MQ=60)

                                                GGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGT                                                                                                       <  SRR19973063.2429270/150‑1 (MQ=60)

                                                  TGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTAC                                                                                                     <  SRR19973063.905390/150‑1 (MQ=60)

                                                     AATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTccgct                                                                                                  >  SRR19973063.4336320/1‑145 (MQ=60)

                                                     AATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCA                                                                                                  <  SRR19973063.2278202/150‑1 (MQ=60)

                                                     AATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCA                                                                                                  >  SRR19973063.4336611/1‑150 (MQ=60)

                                                     AATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCA                                                                                                  >  SRR19973063.1557968/1‑150 (MQ=60)

                                                      ATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAG                                                                                                 >  SRR19973063.3358073/1‑150 (MQ=60)

                                                       TTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGT                                                                                                <  SRR19973063.4264093/150‑1 (MQ=60)

                                                       TTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGT                                                                                                <  SRR19973063.4520481/150‑1 (MQ=60)

                                                            TACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATACCAACCAACGGTGGTACGCAGTTTGGC                                                                                           >  SRR19973063.3130993/1‑150 (MQ=60)

                                                             ACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCC                                                                                          >  SRR19973063.603540/1‑150 (MQ=60)

                                                             ACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCC                                                                                          >  SRR19973063.603455/1‑150 (MQ=60)

                                                             ACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCC                                                                                          >  SRR19973063.4682814/1‑150 (MQ=60)

                                                              CAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCC                                                                                         <  SRR19973063.3488212/150‑1 (MQ=60)

                                                                GCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCAT                                                                                       >  SRR19973063.3138889/1‑150 (MQ=60)

                                                                  CTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGC                                                                                     >  SRR19973063.2860982/1‑150 (MQ=60)

                                                                  CTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGC                                                                                     >  SRR19973063.2860858/1‑150 (MQ=60)

                                                                  CTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGC                                                                                     >  SRR19973063.2860843/1‑150 (MQ=60)

                                                                   TGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCG                                                                                    <  SRR19973063.3818843/150‑1 (MQ=60)

                                                                    GGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGA                                                                                   <  SRR19973063.4250439/150‑1 (MQ=60)

                                                                        AATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGATGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTG                                                                               >  SRR19973063.3898199/1‑150 (MQ=60)

                                                                        AATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGATGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTG                                                                               >  SRR19973063.3898187/1‑150 (MQ=60)

                                                                        AATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTG                                                                               >  SRR19973063.4957466/1‑150 (MQ=60)

                                                                        AATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTG                                                                               <  SRR19973063.4262765/150‑1 (MQ=60)

                                                                        AATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTG                                                                               >  SRR19973063.4957405/1‑150 (MQ=60)

                                                                             GTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTAT                                                                          >  SRR19973063.4487369/1‑150 (MQ=60)

                                                                               CCTAATCCGCCTCGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCC                                                                        <  SRR19973063.4169113/150‑1 (MQ=60)

                                                                               CCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCC                                                                        <  SRR19973063.793294/150‑1 (MQ=60)

                                                                                 TAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCAT                                                                      >  SRR19973063.150475/1‑150 (MQ=60)

                                                                                 TAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCAT                                                                      >  SRR19973063.4452709/1‑150 (MQ=60)

                                                                                   ATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATAT                                                                    >  SRR19973063.2041626/1‑150 (MQ=60)

                                                                                    TCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATC                                                                   >  SRR19973063.193945/1‑150 (MQ=60)

                                                                                    TCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATC                                                                   >  SRR19973063.3555277/1‑150 (MQ=60)

                                                                                    TCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATC                                                                   >  SRR19973063.4236016/1‑150 (MQ=60)

                                                                                     CCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCC                                                                  >  SRR19973063.2558418/1‑150 (MQ=60)

                                                                                      CGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCC                                                                 <  SRR19973063.4259804/150‑1 (MQ=60)

                                                                                      CGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCC                                                                 >  SRR19973063.590834/1‑150 (MQ=60)

                                                                                      CGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCC                                                                 >  SRR19973063.1670202/1‑150 (MQ=60)

                                                                                       GCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCA                                                                >  SRR19973063.901527/1‑150 (MQ=60)

                                                                                        CCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCAT                                                               >  SRR19973063.365640/1‑150 (MQ=60)

                                                                                         CACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTC‑‑‑‑‑‑‑‑‑‑‑TGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAG                                                   >  SRR19973063.4082651/1‑150 (MQ=60)

                                                                                          ACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGC                                                             >  SRR19973063.2570424/1‑150 (MQ=60)

                                                                                            GGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCACCGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCA                                                           <  SRR19973063.2206627/150‑1 (MQ=60)

                                                                                                 CAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCA                                                      >  SRR19973063.1294856/1‑150 (MQ=60)

                                                                                                  AACGCCCTGTAATATCACCATATGTCCATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGATGCGTATCCATATCCCATGACAGACCAT                                                     >  SRR19973063.2771400/1‑150 (MQ=60)

                                                                                                  AACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCAT                                                     <  SRR19973063.3724421/150‑1 (MQ=60)

                                                                                                    CGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAG                                                   >  SRR19973063.2280671/1‑150 (MQ=60)

                                                                                                    CGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAG                                                   >  SRR19973063.2280632/1‑150 (MQ=60)

                                                                                                       CCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGAT                                                >  SRR19973063.3969460/1‑150 (MQ=60)

                                                                                                        CTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATT                                               <  SRR19973063.5011473/150‑1 (MQ=60)

                                                                                                          GTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTA                                             <  SRR19973063.1486512/150‑1 (MQ=60)

                                                                                                          GTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTA                                             >  SRR19973063.1439595/1‑150 (MQ=60)

                                                                                                             ATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTA                                          <  SRR19973063.4038130/150‑1 (MQ=60)

                                                                                                             ATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTA                                          >  SRR19973063.4421735/1‑150 (MQ=60)

                                                                                                             ATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTA                                          >  SRR19973063.528704/1‑150 (MQ=60)

                                                                                                                   CCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATT                                    <  SRR19973063.724689/150‑1 (MQ=60)

                                                                                                                   CCATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATT                                    <  SRR19973063.724761/150‑1 (MQ=60)

                                                                                                                    CATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTT                                   <  SRR19973063.1120173/150‑1 (MQ=60)

                                                                                                                    CATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTT                                   <  SRR19973063.300171/150‑1 (MQ=60)

                                                                                                                     cttccgatcTGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTC                                  <  SRR19973063.2474148/141‑1 (MQ=60)

                                                                                                                     ATATGTACATTAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTC                                  <  SRR19973063.3958330/150‑1 (MQ=60)

                                                                                                                     ATATGTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTC                                  >  SRR19973063.5130981/1‑150 (MQ=60)

                                                                                                                         GTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGA                              >  SRR19973063.4874697/1‑150 (MQ=60)

                                                                                                                         GTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGA                              >  SRR19973063.4870982/1‑150 (MQ=60)

                                                                                                                         GTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGA                              >  SRR19973063.1059316/1‑150 (MQ=60)

                                                                                                                         GTACATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGA                              >  SRR19973063.1079817/1‑150 (MQ=60)

                                                                                                                             ATGAATGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAG                          <  SRR19973063.1792316/150‑1 (MQ=60)

                                                                                                                             ATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAG                          >  SRR19973063.5165174/1‑150 (MQ=60)

                                                                                                                             ATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAG                          <  SRR19973063.1792366/150‑1 (MQ=60)

                                                                                                                             ATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAG                          <  SRR19973063.1792249/150‑1 (MQ=60)

                                                                                                                             ATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAG                          >  SRR19973063.3298775/1‑150 (MQ=60)

                                                                                                                             ATGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAG                          >  SRR19973063.3298808/1‑150 (MQ=60)

                                                                                                                              TGAAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGA                         >  SRR19973063.4332211/1‑150 (MQ=60)

                                                                                                                                AAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACT                       >  SRR19973063.4246534/1‑150 (MQ=60)

                                                                                                                                AAAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACT                       >  SRR19973063.512713/1‑150 (MQ=60)

                                                                                                                                 AAGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTG                      >  SRR19973063.408604/1‑150 (MQ=60)

                                                                                                                                  AGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGC                     >  SRR19973063.4643209/1‑150 (MQ=60)

                                                                                                                                  AGTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGC                     >  SRR19973063.4643129/1‑150 (MQ=60)

                                                                                                                                   GTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCG                    >  SRR19973063.4157823/1‑150 (MQ=60)

                                                                                                                                   GTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCG                    >  SRR19973063.4354164/1‑150 (MQ=60)

                                                                                                                                   GTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCG                    >  SRR19973063.4354165/1‑150 (MQ=60)

                                                                                                                                   GTTCGGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCG                    >  SRR19973063.3094591/1‑150 (MQ=60)

                                                                                                                                       GGTGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATAAGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGACAGACCATAGGATTTATTACCCATTTCGCGATCAGACagcgataa                >  SRR19973063.4768244/1‑142 (MQ=60)

                                                                                                                                         TGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCGGGATGG              <  SRR19973063.4605780/150‑1 (MQ=60)

                                                                                                                                         TGTACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCGGGAAGG              >  SRR19973063.254087/1‑150 (MQ=60)

                                                                                                                                           TACCAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCGGGAAGGTG            <  SRR19973063.1456198/150‑1 (MQ=60)

                                                                                                                                              CAGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCGGGAAGGTGCTG         <  SRR19973063.2415222/150‑1 (MQ=60)

                                                                                                                                               AGATGTAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCGGGAAGGTGCTGG        >  SRR19973063.2632852/1‑150 (MQ=60)

                                                                                                                                                    TAATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCGGGAAGGTGCTGGTATTA   >  SRR19973063.1453829/1‑150 (MQ=60)

                                                                                                                                                     AATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCGGGAAGGTGCTGGTATTAT  >  SRR19973063.1449600/1‑150 (MQ=60)

                                                                                                                                                     AATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCGGGAAGGTGCTGGTATTAT  >  SRR19973063.2451625/1‑150 (MQ=60)

CGAAACATTACCCACGGCGAATTTTTGCCAGTCCAGGCGTGTTTTGAAGGTGTAATTATCTACAGCCTGGGAAATGTGTCCTAATCCGCCACGGGTGCAACGCCCTGTAATATCACCATATGTACATGAAAGTTCGGTGTACCAGATGTCATGATCGTGACGGGTATAATCACTAATATGATCCCAACCAACGGTGGTACGCAGTTTGGCCCATGCGAGCTGCGTATCCATATCCCATGCCAGACCATAGGATTTATTACCCATTTCGCGATCAGACTGCGGGAAGGTGCTGGTATTAT  >  CP009273/1570677‑1570975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`