Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,342,897 C→A 100% D19Y (GAC→TAC)  inaA ← acid‑inducible Kdo/WaaP family putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,342,8970CA99.5% 883.2 / ‑4.3 269D19Y (GAC→TAC) inaAacid‑inducible Kdo/WaaP family putative kinase
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base A (115/151);  total (115/154)
Fisher's exact test for biased strand distribution p-value = 2.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 269 total aligned reads displayed.

GGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTCGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAATTTCT  >  CP009273/2342750‑2343043
                                                                                                                                                   |                                                                                                                                                  
ggACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGc                                                                                                                                                  <  2:3260374/150‑1 (MQ=255)
 gACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGcc                                                                                                                                                 >  1:1043067/1‑150 (MQ=255)
  aCAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGccc                                                                                                                                                <  2:2148779/150‑1 (MQ=255)
  aCAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGccc                                                                                                                                                <  2:3189754/150‑1 (MQ=255)
   cAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCct                                                                                                                                               >  2:3127789/1‑150 (MQ=255)
   cAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCct                                                                                                                                               >  2:85938/1‑150 (MQ=255)
    aaTCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCctc                                                                                                                                              <  1:1519362/150‑1 (MQ=255)
      tCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTg                                                                                                                                            <  2:2500083/150‑1 (MQ=255)
        gTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGtt                                                                                                                                          <  2:3735672/150‑1 (MQ=255)
         ttGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTGTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTAATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTg                                                                                                                                         <  2:3027520/150‑1 (MQ=255)
            gTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGccc                                                                                                                                      >  2:3076821/1‑150 (MQ=255)
             tCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCcca                                                                                                                                     <  2:377853/150‑1 (MQ=255)
                gCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCccacca                                                                                                                                  <  2:4742999/150‑1 (MQ=255)
                  cGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGt                                                                                                                                >  2:4549279/1‑150 (MQ=255)
                  cGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGt                                                                                                                                >  1:2132041/1‑150 (MQ=255)
                    aaCGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGa                                                                                                                              >  1:4647518/1‑150 (MQ=255)
                    aaCGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGa                                                                                                                              >  1:3502872/1‑150 (MQ=255)
                       ggATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTa                                                                                                                           >  1:4926470/1‑150 (MQ=255)
                         aTAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTaaa                                                                                                                         >  2:2580401/1‑150 (MQ=255)
                             cGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATtcg                                                                                                                     <  2:1311723/150‑1 (MQ=255)
                              gTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATtcgt                                                                                                                    >  2:565823/1‑150 (MQ=255)
                              gTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATtcgt                                                                                                                    <  1:532821/150‑1 (MQ=255)
                                  ggAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTa                                                                                                                >  1:58912/1‑150 (MQ=255)
                                     aTGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACtt                                                                                                             <  1:1185714/150‑1 (MQ=255)
                                      tGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACttt                                                                                                            >  1:1947303/1‑150 (MQ=255)
                                      tGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACttt                                                                                                            <  2:3643845/150‑1 (MQ=255)
                                        aaaCAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGc                                                                                                          >  1:969987/1‑150 (MQ=255)
                                        aaaCAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGc                                                                                                          >  1:1291718/1‑150 (MQ=255)
                                         aaCAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCt                                                                                                         >  1:1076667/1‑150 (MQ=255)
                                           cAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGa                                                                                                       >  2:1721460/1‑150 (MQ=255)
                                            aGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGaa                                                                                                      >  1:2879454/1‑150 (MQ=255)
                                            aGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGaa                                                                                                      <  1:1660343/150‑1 (MQ=255)
                                               tgatgCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACt                                                                                                   <  1:890362/150‑1 (MQ=255)
                                               tgatgCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACt                                                                                                   <  1:305956/150‑1 (MQ=255)
                                                  tgCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGcc                                                                                                <  1:4267690/150‑1 (MQ=255)
                                                  tgCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGcc                                                                                                <  2:1057905/150‑1 (MQ=255)
                                                     gTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATa                                                                                             <  1:1685599/150‑1 (MQ=255)
                                                     gTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATa                                                                                             <  2:4146147/150‑1 (MQ=255)
                                                      tCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATAc                                                                                            >  2:1207810/1‑150 (MQ=255)
                                                       cATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATAcc                                                                                           <  2:2844140/150‑1 (MQ=255)
                                                        aTGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATAccc                                                                                          <  2:2244684/150‑1 (MQ=255)
                                                        aTGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATAccc                                                                                          >  2:2171458/1‑150 (MQ=255)
                                                        aTGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATAccc                                                                                          <  2:4340057/150‑1 (MQ=255)
                                                        aTGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATAccc                                                                                          >  1:3009146/1‑150 (MQ=255)
                                                         tGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCt                                                                                         >  2:4427896/1‑150 (MQ=255)
                                                         tGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCt                                                                                         >  1:488543/1‑150 (MQ=255)
                                                          gcgcTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTa                                                                                        >  1:1849808/1‑150 (MQ=255)
                                                           cgcTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTAt                                                                                       <  2:4863733/150‑1 (MQ=255)
                                                            gcTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATc                                                                                      <  1:2932713/150‑1 (MQ=255)
                                                                 acatacaGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTg                                                                                 <  1:2011350/150‑1 (MQ=255)
                                                                 acatacaGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTg                                                                                 <  2:2360333/150‑1 (MQ=255)
                                                                  catacaGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGt                                                                                <  2:2019180/150‑1 (MQ=255)
                                                                   atacaGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTc                                                                               <  2:2129079/150‑1 (MQ=255)
                                                                       aGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCAttt                                                                           >  2:2960668/1‑150 (MQ=255)
                                                                        gCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCAtttt                                                                          <  2:2751724/150‑1 (MQ=255)
                                                                         cTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCAttttt                                                                         <  2:4826405/150‑1 (MQ=255)
                                                                           ttttGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTttat                                                                       >  2:2827731/1‑150 (MQ=255)
                                                                             ttGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTttatta                                                                     <  1:544906/150‑1 (MQ=255)
                                                                              tGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTaa                                                                    >  1:49435/1‑150 (MQ=255)
                                                                                ccGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACg                                                                  >  2:3510677/1‑150 (MQ=255)
                                                                                 cGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGa                                                                 >  1:2970365/1‑150 (MQ=255)
                                                                                  gTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGAc                                                                <  2:140136/150‑1 (MQ=255)
                                                                                     gcgcTCGACTCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTTATTAAATTCGTCGTACTTTGCTGAAACTGCAATACCCTATCGCCTGTCATTTTTATTAACGACAAt                                                             <  2:3938870/150‑1 (MQ=255)
                                                                                        cTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGAc                                                          >  1:1451078/1‑150 (MQ=255)
                                                                                         ccGACGCACTGCACCCCGCTCCTACCATCACGGCGATAGTTAGGCTCTTCAACCCAGTCGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACt                                                         <  1:2685124/149‑1 (MQ=255)
                                                                                          cGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACta                                                        >  2:3298564/1‑150 (MQ=255)
                                                                                           gACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACtat                                                       >  1:1031792/1‑150 (MQ=255)
                                                                                              gCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAgg                                                    <  1:1268455/150‑1 (MQ=255)
                                                                                                aTTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAggtg                                                  >  2:1109373/1‑150 (MQ=255)
                                                                                                  tGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAggtggt                                                >  2:3341070/1‑150 (MQ=255)
                                                                                                   gCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGtt                                               <  2:3831888/150‑1 (MQ=255)
                                                                                                   gCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGtt                                               >  1:336689/1‑150 (MQ=255)
                                                                                                      ccccGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTAcc                                            >  2:1012894/1‑150 (MQ=255)
                                                                                                       cccGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCt                                           >  1:720422/1‑150 (MQ=255)
                                                                                                          gCTCATACCTTTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTTTTGCCCACCAGTGATTAATTTCGTCGTACTGTGCTGAAACTGCCATACCCTATCGCCTTTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAg                                        <  1:3127789/150‑1 (MQ=255)
                                                                                                           cTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAgg                                       <  2:2960830/150‑1 (MQ=255)
                                                                                                           cTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAgg                                       <  1:5110982/150‑1 (MQ=255)
                                                                                                              aTACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGaaa                                    >  2:1979304/1‑150 (MQ=255)
                                                                                                                 ccATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATc                                 <  2:944745/150‑1 (MQ=255)
                                                                                                                  cATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCt                                <  1:841984/150‑1 (MQ=255)
                                                                                                                   aTTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCtt                               <  2:5014071/150‑1 (MQ=255)
                                                                                                                   aTTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCtt                               >  1:2072533/1‑150 (MQ=255)
                                                                                                                    ttACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTa                              <  2:4926416/150‑1 (MQ=255)
                                                                                                                     tACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTaa                             >  2:4705588/1‑150 (MQ=255)
                                                                                                                       cGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATg                           >  2:2900783/1‑150 (MQ=255)
                                                                                                                        ggCGATAGTTAGGCACTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGa                          <  2:3487324/150‑1 (MQ=255)
                                                                                                                          cGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGaaa                        >  1:1418921/1‑150 (MQ=255)
                                                                                                                            aTAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACg                      >  2:2651766/1‑150 (MQ=255)
                                                                                                                              aGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACgtg                    <  2:1576553/150‑1 (MQ=255)
                                                                                                                              aGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCGGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACgtg                    <  2:1341794/150‑1 (MQ=255)
                                                                                                                                ttAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTc                  >  2:1191758/1‑150 (MQ=255)
                                                                                                                                ttAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTc                  >  1:295402/1‑150 (MQ=255)
                                                                                                                                 tAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCg                 <  2:759329/150‑1 (MQ=255)
                                                                                                                                  aGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGt                >  1:3945977/1‑150 (MQ=255)
                                                                                                                                  aGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGt                >  2:2314231/1‑150 (MQ=255)
                                                                                                                                     ctctTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTAtt             <  1:4506897/150‑1 (MQ=255)
                                                                                                                                     ctctTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTAtt             <  1:3575643/150‑1 (MQ=255)
                                                                                                                                           aaCCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGaa       >  1:4541986/1‑150 (MQ=255)
                                                                                                                                           aaCCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGaa       >  1:2503609/1‑150 (MQ=255)
                                                                                                                                                aGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAATTTCt  >  1:402668/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTCGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAATTTCT  >  CP009273/2342750‑2343043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTCGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAATTTCTTTT  >  CP009273/2342749‑2343046
                                                                                                                                                    |                                                                                                                                                     
CGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAG                                                                                                                                                      <  SRR19973063.2017889/150‑1 (MQ=60)
CGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAG                                                                                                                                                      <  SRR19973063.4814957/150‑1 (MQ=60)
CGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAG                                                                                                                                                      >  SRR19973063.3015846/1‑150 (MQ=60)
CGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAG                                                                                                                                                      <  SRR19973063.1160145/150‑1 (MQ=60)
  GACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCC                                                                                                                                                    >  SRR19973063.1046226/1‑150 (MQ=60)
   ACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCC                                                                                                                                                   <  SRR19973063.2880347/150‑1 (MQ=60)
   ACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCC                                                                                                                                                   <  SRR19973063.573422/150‑1 (MQ=60)
    CAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCT                                                                                                                                                  <  SRR19973063.4405167/150‑1 (MQ=60)
     AATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTC                                                                                                                                                 <  SRR19973063.1524022/150‑1 (MQ=60)
           TGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGC                                                                                                                                           >  SRR19973063.3651899/1‑150 (MQ=60)
             GTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCC                                                                                                                                         <  SRR19973063.4301845/150‑1 (MQ=60)
                   CGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGT                                                                                                                                   >  SRR19973063.2138702/1‑150 (MQ=60)
                     AACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGA                                                                                                                                 >  SRR19973063.3514069/1‑150 (MQ=60)
                     AACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGA                                                                                                                                 >  SRR19973063.4662361/1‑150 (MQ=60)
                        GGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTA                                                                                                                              >  SRR19973063.4942383/1‑150 (MQ=60)
                        GGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTA                                                                                                                              >  SRR19973063.4942327/1‑150 (MQ=60)
                              CGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCG                                                                                                                        >  SRR19973063.1496901/1‑150 (MQ=60)
                               GTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGT                                                                                                                       <  SRR19973063.534349/150‑1 (MQ=60)
                               GTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGT                                                                                                                       <  SRR19973063.534390/150‑1 (MQ=60)
                                TACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTC                                                                                                                      <  SRR19973063.3008779/150‑1 (MQ=60)
                                TACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTC                                                                                                                      <  SRR19973063.3010958/150‑1 (MQ=60)
                                TACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTC                                                                                                                      >  SRR19973063.142367/1‑150 (MQ=60)
                                  CGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGT                                                                                                                    <  SRR19973063.1629621/150‑1 (MQ=60)
                                   GGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTA                                                                                                                   >  SRR19973063.59119/1‑150 (MQ=60)
                                   GGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTA                                                                                                                   >  SRR19973063.2949128/1‑150 (MQ=60)
                                     AATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACT                                                                                                                 <  SRR19973063.1824820/150‑1 (MQ=60)
                                      ATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTT                                                                                                                <  SRR19973063.1189281/150‑1 (MQ=60)
                                       TGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTT                                                                                                               >  SRR19973063.1953400/1‑150 (MQ=60)
                                        GAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTG                                                                                                              <  SRR19973063.3356222/150‑1 (MQ=60)
                                         AAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGC                                                                                                             >  SRR19973063.972910/1‑150 (MQ=60)
                                         AAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGC                                                                                                             >  SRR19973063.1295566/1‑150 (MQ=60)
                                          AACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCT                                                                                                            >  SRR19973063.1079934/1‑150 (MQ=60)
                                          AACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCT                                                                                                            >  SRR19973063.1079918/1‑150 (MQ=60)
                                             AGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAA                                                                                                         <  SRR19973063.1665403/150‑1 (MQ=60)
                                             AGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAA                                                                                                         >  SRR19973063.2888663/1‑150 (MQ=60)
                                              GATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAA                                                                                                        >  SRR19973063.3149910/1‑150 (MQ=60)
                                               ATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAAC                                                                                                       <  SRR19973063.893065/150‑1 (MQ=60)
                                               ATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAAC                                                                                                       <  SRR19973063.4632724/150‑1 (MQ=60)
                                                TGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACT                                                                                                      <  SRR19973063.306853/150‑1 (MQ=60)
                                                TGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACT                                                                                                      <  SRR19973063.892942/150‑1 (MQ=60)
                                                TGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACT                                                                                                      <  SRR19973063.2908422/150‑1 (MQ=60)
                                                   TGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCC                                                                                                   <  SRR19973063.4281261/150‑1 (MQ=60)
                                                   TGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCC                                                                                                   <  SRR19973063.4554087/150‑1 (MQ=60)
                                                      GTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATA                                                                                                <  SRR19973063.1690732/150‑1 (MQ=60)
                                                         ATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCAtaggtcgtcgttaatcacacagcgggtcgcccttgtatctgtt                                                                                             >  SRR19973063.2308958/1‑107 (MQ=60)
                                                         ATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCC                                                                                             <  SRR19973063.4007873/150‑1 (MQ=60)
                                                         ATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCC                                                                                             >  SRR19973063.3018748/1‑150 (MQ=60)
                                                          TGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCT                                                                                            >  SRR19973063.489940/1‑150 (MQ=60)
                                                           GCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTA                                                                                           <  SRR19973063.134993/150‑1 (MQ=60)
                                                           GCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTA                                                                                           <  SRR19973063.134972/150‑1 (MQ=60)
                                                           GCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTA                                                                                           >  SRR19973063.3774159/1‑150 (MQ=60)
                                                           GCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTA                                                                                           >  SRR19973063.1855580/1‑150 (MQ=60)
                                                           GCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTA                                                                                           <  SRR19973063.3408432/150‑1 (MQ=60)
                                                             GCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATC                                                                                         <  SRR19973063.2942062/150‑1 (MQ=60)
                                                             GCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATC                                                                                         >  SRR19973063.1201391/1‑150 (MQ=60)
                                                                  ACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTG                                                                                    <  SRR19973063.2017631/150‑1 (MQ=60)
                                                                  ACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTG                                                                                    <  SRR19973063.2114759/150‑1 (MQ=60)
                                                                    ATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTC                                                                                  >  SRR19973063.2076759/1‑150 (MQ=60)
                                                                     TACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCA                                                                                 <  SRR19973063.1292141/150‑1 (MQ=60)
                                                                     TACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCA                                                                                 <  SRR19973063.3087654/150‑1 (MQ=60)
                                                                     TACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCA                                                                                 <  SRR19973063.1291990/150‑1 (MQ=60)
                                                                     TACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCA                                                                                 >  SRR19973063.2277598/1‑150 (MQ=60)
                                                                     TACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATAACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCA                                                                                 >  SRR19973063.3033495/1‑150 (MQ=60)
                                                                       CAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATT                                                                               <  SRR19973063.234087/150‑1 (MQ=60)
                                                                       CAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATT                                                                               <  SRR19973063.4919519/150‑1 (MQ=60)
                                                                        AGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTT                                                                              <  SRR19973063.949961/150‑1 (MQ=60)
                                                                             TTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTTCCATACCCTATCGCCTGTCATTTTTATT                                                                         >  SRR19973063.1867023/1‑150 (MQ=60)
                                                                              TTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTA                                                                        <  SRR19973063.546517/150‑1 (MQ=60)
                                                                              TTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTA                                                                        <  SRR19973063.546251/150‑1 (MQ=60)
                                                                               TGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAA                                                                       >  SRR19973063.49587/1‑150 (MQ=60)
                                                                               TGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAA                                                                       >  SRR19973063.699364/1‑150 (MQ=60)
                                                                               TGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAA                                                                       >  SRR19973063.1903320/1‑150 (MQ=60)
                                                                               TGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAA                                                                       >  SRR19973063.49610/1‑150 (MQ=60)
                                                                                  CGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGA                                                                    >  SRR19973063.2979861/1‑150 (MQ=60)
                                                                                  CGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGA                                                                    >  SRR19973063.2979561/1‑150 (MQ=60)
                                                                                         CTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGAC                                                             >  SRR19973063.1455505/1‑150 (MQ=60)
                                                                                          TCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACT                                                            <  SRR19973063.3534404/150‑1 (MQ=60)
                                                                                          CCGACGCACTGCACCCCGCTCCTACCATCACGGCGATAGTTAGGCTCTTCAACCCAGTCGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACT                                                            <  SRR19973063.2693775/150‑1 (MQ=60)
                                                                                           CGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTA                                                           >  SRR19973063.5030259/1‑150 (MQ=60)
                                                                                            GACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTAT                                                          >  SRR19973063.1520490/1‑150 (MQ=60)
                                                                                            GACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTAT                                                          <  SRR19973063.1142989/150‑1 (MQ=60)
                                                                                            GACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTAT                                                          >  SRR19973063.1034908/1‑150 (MQ=60)
                                                                                              CGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAG                                                        <  SRR19973063.991857/150‑1 (MQ=60)
                                                                                               GCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGG                                                       <  SRR19973063.1272224/150‑1 (MQ=60)
                                                                                                CATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGT                                                      <  SRR19973063.4208638/150‑1 (MQ=60)
                                                                                                  TTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGG                                                    <  SRR19973063.471232/150‑1 (MQ=60)
                                                                                                   TGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGT                                                   >  SRR19973063.2608736/1‑150 (MQ=60)
                                                                                                    GCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTT                                                  >  SRR19973063.337663/1‑150 (MQ=60)
                                                                                                        CCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCT                                              >  SRR19973063.722542/1‑150 (MQ=60)
                                                                                                           GCTCATACCTTTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTTTTGCCCACCAGTGATTAATTTCGTCGTACTGTGCTGAAACTGCCATACCCTATCGCCTTTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAG                                           <  SRR19973063.3137858/150‑1 (MQ=60)
                                                                                                           GCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAG                                           <  SRR19973063.517858/150‑1 (MQ=60)
                                                                                                            CTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGG                                          <  SRR19973063.5127426/150‑1 (MQ=60)
                                                                                                              CATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAA                                        <  SRR19973063.4664181/150‑1 (MQ=60)
                                                                                                               ATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATATGTGGTTACCTGAGGAAA                                       >  SRR19973063.4032061/1‑150 (MQ=60)
                                                                                                                   CATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCT                                   <  SRR19973063.844417/150‑1 (MQ=60)
                                                                                                                   CATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCT                                   <  SRR19973063.844435/150‑1 (MQ=60)
                                                                                                                    ATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTT                                  >  SRR19973063.2079012/1‑150 (MQ=60)
                                                                                                                    ATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTT                                  >  SRR19973063.2079049/1‑150 (MQ=60)
                                                                                                                      TACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAA                                >  SRR19973063.1036723/1‑150 (MQ=60)
                                                                                                                      TACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAA                                <  SRR19973063.2914061/150‑1 (MQ=60)
                                                                                                                       ACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAAT                               >  SRR19973063.4541767/1‑150 (MQ=60)
                                                                                                                        CGGCGATAGTTAGGCTCTTCAACCCAGTCGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATG                              <  SRR19973063.2037905/150‑1 (MQ=60)
                                                                                                                        CGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATG                              <  SRR19973063.2006978/150‑1 (MQ=60)
                                                                                                                           CGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAA                           >  SRR19973063.1423234/1‑150 (MQ=60)
                                                                                                                            GATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAAC                          <  SRR19973063.553884/150‑1 (MQ=60)
                                                                                                                             ATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACG                         <  SRR19973063.86231/150‑1 (MQ=60)
                                                                                                                               AGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTG                       <  SRR19973063.287990/150‑1 (MQ=60)
                                                                                                                                GTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGT                      <  SRR19973063.724563/150‑1 (MQ=60)
                                                                                                                                 TTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTC                     >  SRR19973063.296274/1‑150 (MQ=60)
                                                                                                                                 TTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTC                     >  SRR19973063.296345/1‑150 (MQ=60)
                                                                                                                                 TTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCATGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTC                     >  SRR19973063.1611767/1‑150 (MQ=60)
                                                                                                                                  TAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCG                    >  SRR19973063.3121403/1‑150 (MQ=60)
                                                                                                                                   AGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTAGT                   >  SRR19973063.3958598/1‑150 (MQ=60)
                                                                                                                                    GGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGAACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTA                  >  SRR19973063.1179384/1‑150 (MQ=60)
                                                                                                                                     GCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTAT                 <  SRR19973063.1066013/150‑1 (MQ=60)
                                                                                                                                      CTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATT                <  SRR19973063.3587113/150‑1 (MQ=60)
                                                                                                                                      CTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATT                <  SRR19973063.4521361/150‑1 (MQ=60)
                                                                                                                                            AACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAA          >  SRR19973063.5078099/1‑150 (MQ=60)
                                                                                                                                            AACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAA          >  SRR19973063.1688630/1‑150 (MQ=60)
                                                                                                                                            AACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAA          >  SRR19973063.2511595/1‑150 (MQ=60)
                                                                                                                                            AACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAA          >  SRR19973063.4556548/1‑150 (MQ=60)
                                                                                                                                             ACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAAT         <  SRR19973063.1735382/150‑1 (MQ=60)
                                                                                                                                              CCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAATT        <  SRR19973063.3544479/150‑1 (MQ=60)
                                                                                                                                               CCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAATTT       >  SRR19973063.687023/1‑150 (MQ=60)
                                                                                                                                                 AGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAATTTCT     >  SRR19973063.403805/1‑150 (MQ=60)
                                                                                                                                                  GTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAATTTCTT    <  SRR19973063.1476868/150‑1 (MQ=60)
                                                                                                                                                    AGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAATTTCTTTT  <  SRR19973063.2873859/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTCGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAATTTCTTTT  >  CP009273/2342749‑2343046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑