Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F488 I0 R1	1275	300.1	10328444	91.0%	9398884	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,210,753	C→A	100%	E555* (GAA→TAA)	arpA ←	ankyrin repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,210,753	0	C	A	98.3%	745.2 / 0.4	229	E555* (GAA→TAA)	arpA	ankyrin repeat protein
Reads supporting (aligned to +/- strand): ref base C (3/1); new base A (95/130); total (98/131)
Fisher's exact test for biased strand distribution p-value = 3.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 229 total aligned reads displayed.

ACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGC  >  CP009273/4210606‑4210899

aCGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACt                                                                                                                                                  >  1:3865280/1‑150 (MQ=255)

 cGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTg                                                                                                                                                 >  2:1567722/1‑150 (MQ=255)

  ggaTTAAACAGAGTGGGTAAGGCGTTAAATATAGTATGAATAACGTTCATGTGTCCGCGCTGCATGGGCATAAACAAACCCGTGTCGCGCGCTAGCCTGTTAGCGGTCAGAAGAGCTACAATATCGGAAGCTGTCATGTTAATTTCCTga                                                                                                                                                >  2:2613054/3‑149 (MQ=255)

       aaaCAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAg                                                                                                                                           <  1:1973231/150‑1 (MQ=255)

        aaCAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAgg                                                                                                                                          >  2:1430894/1‑150 (MQ=255)

          cAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCt                                                                                                                                        <  1:3978314/150‑1 (MQ=255)

           agagTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTg                                                                                                                                       >  1:1131049/1‑150 (MQ=255)

             agTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTggg                                                                                                                                     >  1:242261/1‑150 (MQ=255)

             agTGGGTAATGCGGTAAAAATAGTATTAATAACGTTCATGTGTCCGAGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTggg                                                                                                                                     >  2:1608874/1‑150 (MQ=255)

               tGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCAATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGACGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCa                                                                                                                                   >  2:2216282/1‑150 (MQ=255)

                gggTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCaa                                                                                                                                  <  1:935385/150‑1 (MQ=255)

                 ggTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAAt                                                                                                                                 <  2:3833547/150‑1 (MQ=255)

                  gTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATg                                                                                                                                <  1:3009821/150‑1 (MQ=255)

                   tAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGc                                                                                                                               <  1:5071928/150‑1 (MQ=255)

                    aaTGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCt                                                                                                                              <  1:1788312/150‑1 (MQ=255)

                    aaTGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCt                                                                                                                              <  2:3234442/150‑1 (MQ=255)

                      tGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTc                                                                                                                            <  2:2909820/150‑1 (MQ=255)

                      tGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTc                                                                                                                            <  1:2612643/150‑1 (MQ=255)

                      tGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTc                                                                                                                            >  2:3704026/1‑150 (MQ=255)

                       gCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCa                                                                                                                           <  1:4955027/150‑1 (MQ=255)

                        cGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCaa                                                                                                                          <  2:1177870/150‑1 (MQ=255)

                         gTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAg                                                                                                                         <  2:2711787/150‑1 (MQ=255)

                         gTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAg                                                                                                                         <  2:5163400/150‑1 (MQ=255)

                                    tATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCa                                                                                                              >  2:728796/1‑150 (MQ=255)

                                     aTTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCAc                                                                                                             <  1:4409482/150‑1 (MQ=255)

                                       taataaCGTTCATGTTTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGa                                                                                                           <  2:3342934/150‑1 (MQ=255)

                                       taataaCGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGa                                                                                                           >  2:2335378/1‑150 (MQ=255)

                                       taataaCGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGa                                                                                                           >  2:2793169/1‑150 (MQ=255)

                                        aataaCGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGat                                                                                                          >  1:2190356/1‑150 (MQ=255)

                                        aataaCGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGat                                                                                                          >  1:2944406/1‑150 (MQ=255)

                                              gTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCt                                                                                                    <  1:4333907/150‑1 (MQ=255)

                                                  aTGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGt                                                                                                >  1:4188463/1‑150 (MQ=255)

                                                   tgtgTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTc                                                                                               <  1:4518488/150‑1 (MQ=255)

                                                     tgtCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCa                                                                                             >  1:2233544/1‑150 (MQ=255)

                                                      gtCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCAt                                                                                            >  1:4826464/1‑150 (MQ=255)

                                                       tCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCAtt                                                                                           <  1:1998132/150‑1 (MQ=255)

                                                           cgcTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGc                                                                                       >  2:4758394/1‑150 (MQ=255)

                                                           cgcTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGc                                                                                       <  1:1757810/150‑1 (MQ=255)

                                                              tGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATc                                                                                    >  1:1386154/1‑150 (MQ=255)

                                                              tGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATc                                                                                    <  2:3627348/150‑1 (MQ=255)

                                                                cATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGc                                                                                  <  1:402357/150‑1 (MQ=255)

                                                                cATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGc                                                                                  >  1:202066/1‑150 (MQ=255)

                                                                cATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGc                                                                                  <  1:62828/150‑1 (MQ=255)

                                                                cATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGc                                                                                  >  1:3125170/1‑150 (MQ=255)

                                                                    gCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGa                                                                              <  2:5086525/150‑1 (MQ=255)

                                                                    gCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGa                                                                              >  2:3100014/1‑150 (MQ=255)

                                                                    gCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGa                                                                              <  2:4850524/150‑1 (MQ=255)

                                                                     ccATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGat                                                                             >  1:2801363/1‑150 (MQ=255)

                                                                      cagaaacaaacCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGata                                                                            <  2:812275/147‑1 (MQ=255)

                                                                      cATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGata                                                                            <  2:1803918/150‑1 (MQ=255)

                                                                       aTAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGatat                                                                           <  1:1354516/150‑1 (MQ=255)

                                                                       aTAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGACCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGatat                                                                           >  1:4084126/1‑150 (MQ=255)

                                                                        tAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGAtata                                                                          <  1:2709681/150‑1 (MQ=255)

                                                                         aaacaaacCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAg                                                                         <  2:4263293/150‑1 (MQ=255)

                                                                         aaacaaacCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAg                                                                         <  2:4875981/150‑1 (MQ=255)

                                                                         aaacaaacCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAg                                                                         >  2:1673351/1‑150 (MQ=255)

                                                                         aaacaaacCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAg                                                                         <  1:2788380/150‑1 (MQ=255)

                                                                         aaacaaacCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAg                                                                         >  2:3881085/1‑150 (MQ=255)

                                                                          aacaaacCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGt                                                                        <  1:2406336/150‑1 (MQ=255)

                                                                           acaaacCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTc                                                                       >  1:549830/1‑150 (MQ=255)

                                                                            caaacCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTcc                                                                      >  1:1022733/1‑150 (MQ=255)

                                                                              aacCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTg                                                                    <  1:3712057/150‑1 (MQ=255)

                                                                                             cAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAAt                                                     <  2:4951250/150‑1 (MQ=255)

                                                                                             cAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAAt                                                     >  2:1993325/1‑150 (MQ=255)

                                                                                              aaGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATc                                                    >  2:889629/1‑150 (MQ=255)

                                                                                               aGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATcc                                                   >  2:2081708/1‑150 (MQ=255)

                                                                                               aGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATcc                                                   >  1:813647/1‑150 (MQ=255)

                                                                                                 aCTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCtt                                                 <  1:3498282/150‑1 (MQ=255)

                                                                                                      tAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCATGGGCAACCATAAAAATCCTTTGCCt                                            >  2:1190838/1‑150 (MQ=255)

                                                                                                       aGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCtt                                           >  1:1940460/1‑150 (MQ=255)

                                                                                                       aGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCtt                                           <  1:4439969/150‑1 (MQ=255)

                                                                                                          ggTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAg                                        >  2:3312223/1‑150 (MQ=255)

                                                                                                              agaagaTCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGgaga                                    >  1:184975/1‑150 (MQ=255)

                                                                                                              agaagaTCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGgaga                                    <  2:3523651/150‑1 (MQ=255)

                                                                                                                aagaTCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATc                                  <  1:3469743/150‑1 (MQ=255)

                                                                                                                  gaTCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGa                                <  2:153783/150‑1 (MQ=255)

                                                                                                                     cTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTa                             <  1:1708475/150‑1 (MQ=255)

                                                                                                                         aaTATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGATTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTg                         <  1:3292705/150‑1 (MQ=255)

                                                                                                                             tCGGAAGCTGAAATGTTACTTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCt                     <  1:1567722/150‑1 (MQ=255)

                                                                                                                              cGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCtt                    >  1:989866/1‑150 (MQ=255)

                                                                                                                              cGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCtt                    <  2:4182207/150‑1 (MQ=255)

                                                                                                                              cGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCtt                    <  2:4412007/150‑1 (MQ=255)

                                                                                                                              cGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCtt                    >  1:3217327/1‑150 (MQ=255)

                                                                                                                                gAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTa                  <  1:728796/150‑1 (MQ=255)

                                                                                                                                    cTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCa              >  1:4861980/1‑150 (MQ=255)

                                                                                                                                    cTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCa              <  1:2537204/150‑1 (MQ=255)

                                                                                                                                     tGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCaa             >  1:4714585/1‑150 (MQ=255)

                                                                                                                                     tGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCaa             >  1:5147536/1‑150 (MQ=255)

                                                                                                                                     tGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGAACGAGTACCAGTTCTTAAGTCaa             >  1:3978056/1‑150 (MQ=255)

                                                                                                                                      gAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCaaa            >  1:4398763/1‑150 (MQ=255)

                                                                                                                                       aaaTGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAAt           <  2:2084394/150‑1 (MQ=255)

                                                                                                                                         aTGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGa         >  2:701342/1‑150 (MQ=255)

                                                                                                                                         aTGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGa         <  1:4281035/150‑1 (MQ=255)

                                                                                                                                            ttAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACAt      <  1:3704026/150‑1 (MQ=255)

                                                                                                                                             tAATTTACTGGTCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACAtg     <  1:1608874/150‑1 (MQ=255)

                                                                                                                                              aaTTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACAtgt    >  2:4054876/1‑150 (MQ=255)

                                                                                                                                              aaTTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACAtgt    >  2:3800506/1‑150 (MQ=255)

                                                                                                                                              aaTTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACAtgt    <  2:1255715/150‑1 (MQ=255)

                                                                                                                                               aTTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACAtgtg   <  2:3670072/150‑1 (MQ=255)

                                                                                                                                               aTTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACAtgtg   <  2:3107934/150‑1 (MQ=255)

ACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGC  >  CP009273/4210606‑4210899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGA  >  CP009273/4210604‑4210901

AAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAGGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTA                                                                                                                                                      >  SRR19973063.3722388/1‑150 (MQ=60)

AAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTA                                                                                                                                                      >  SRR19973063.1129100/1‑150 (MQ=60)

AAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTA                                                                                                                                                      >  SRR19973063.1609495/1‑150 (MQ=60)

 AACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTAC                                                                                                                                                     >  SRR19973063.392006/1‑150 (MQ=60)

  ACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACT                                                                                                                                                    >  SRR19973063.3877575/1‑150 (MQ=60)

     TATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGG                                                                                                                                                 >  SRR19973063.3581576/1‑150 (MQ=60)

     TATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGG                                                                                                                                                 >  SRR19973063.1548517/1‑150 (MQ=60)

        TAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTA                                                                                                                                              <  SRR19973063.2536779/150‑1 (MQ=60)

         AAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAG                                                                                                                                             <  SRR19973063.4317456/150‑1 (MQ=60)

         AAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAG                                                                                                                                             <  SRR19973063.1979400/150‑1 (MQ=60)

           ACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGC                                                                                                                                           >  SRR19973063.2555158/1‑150 (MQ=60)

            CAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCT                                                                                                                                          <  SRR19973063.3991061/150‑1 (MQ=60)

             AGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTG                                                                                                                                         >  SRR19973063.1134480/1‑150 (MQ=60)

              GAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGG                                                                                                                                        <  SRR19973063.1367077/150‑1 (MQ=60)

               AGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGG                                                                                                                                       >  SRR19973063.242987/1‑150 (MQ=60)

               AGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGG                                                                                                                                       >  SRR19973063.242994/1‑150 (MQ=60)

                 TGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCA                                                                                                                                     <  SRR19973063.1712145/150‑1 (MQ=60)

                  GGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAA                                                                                                                                    <  SRR19973063.938173/150‑1 (MQ=60)

                    GTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATG                                                                                                                                  <  SRR19973063.3019426/150‑1 (MQ=60)

                    GTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATG                                                                                                                                  <  SRR19973063.2003964/150‑1 (MQ=60)

                     TAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGC                                                                                                                                 <  SRR19973063.5088241/150‑1 (MQ=60)

                      AATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCT                                                                                                                                <  SRR19973063.1793840/150‑1 (MQ=60)

                      AATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCT                                                                                                                                <  SRR19973063.1793866/150‑1 (MQ=60)

                      AATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCT                                                                                                                                <  SRR19973063.3993533/150‑1 (MQ=60)

                        TGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTC                                                                                                                              <  SRR19973063.2620955/150‑1 (MQ=60)

                         gcaacaaccagagctaatacttttTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCA                                                                                                                             <  SRR19973063.4580267/126‑1 (MQ=60)

                         GCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCA                                                                                                                             <  SRR19973063.4971046/150‑1 (MQ=60)

                          CGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAA                                                                                                                            <  SRR19973063.3281397/150‑1 (MQ=60)

                          CGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAA                                                                                                                            <  SRR19973063.421309/150‑1 (MQ=60)

                           GTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAG                                                                                                                           <  SRR19973063.1743226/150‑1 (MQ=60)

                           GTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAG                                                                                                                           <  SRR19973063.1743177/150‑1 (MQ=60)

                           GTTAAAAATAGTATTAATAACGTTCATGTGTACGCGCTGCATGGCCATAAACAACCCCGTGTCGCGCGCTAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAATTGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAG                                                                                                                           <  SRR19973063.2816255/150‑1 (MQ=60)

                              AAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAAT                                                                                                                        >  SRR19973063.568721/1‑150 (MQ=60)

                                       ATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCAC                                                                                                               <  SRR19973063.372172/150‑1 (MQ=60)

                                       ATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCAC                                                                                                               >  SRR19973063.3505276/1‑150 (MQ=60)

                                       ATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCAC                                                                                                               <  SRR19973063.4423532/150‑1 (MQ=60)

                                       ATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCAC                                                                                                               <  SRR19973063.4423623/150‑1 (MQ=60)

                                          AATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGAT                                                                                                            >  SRR19973063.2953814/1‑150 (MQ=60)

                                          AATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGAT                                                                                                            >  SRR19973063.2197231/1‑150 (MQ=60)

                                           ATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATA                                                                                                           >  SRR19973063.1791473/1‑150 (MQ=60)

                                             AACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATC                                                                                                         <  SRR19973063.2220496/150‑1 (MQ=60)

                                              ACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCG                                                                                                        >  SRR19973063.3724642/1‑150 (MQ=60)

                                               CGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGC                                                                                                       >  SRR19973063.67241/1‑150 (MQ=60)

                                                GTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCT                                                                                                      >  SRR19973063.403305/1‑150 (MQ=60)

                                                GTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCT                                                                                                      <  SRR19973063.4347720/150‑1 (MQ=60)

                                                  TCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTAT                                                                                                    <  SRR19973063.2019023/150‑1 (MQ=60)

                                                   CATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATG                                                                                                   <  SRR19973063.2034922/150‑1 (MQ=60)

                                                    ATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGT                                                                                                  >  SRR19973063.4201837/1‑150 (MQ=60)

                                                    ATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGT                                                                                                  >  SRR19973063.188075/1‑150 (MQ=60)

                                                     TGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTC                                                                                                 <  SRR19973063.4532988/150‑1 (MQ=60)

                                                       TGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCA                                                                                               >  SRR19973063.2240629/1‑150 (MQ=60)

                                                        GTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCAT                                                                                              >  SRR19973063.4841928/1‑150 (MQ=60)

                                                        GTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCAT                                                                                              >  SRR19973063.4842009/1‑150 (MQ=60)

                                                         TCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATT                                                                                             <  SRR19973063.2004374/150‑1 (MQ=60)

                                                             CGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGC                                                                                         <  SRR19973063.1763213/150‑1 (MQ=60)

                                                                TGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATC                                                                                      >  SRR19973063.1390343/1‑150 (MQ=60)

                                                                TGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATC                                                                                      >  SRR19973063.812728/1‑150 (MQ=60)

                                                                  CATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGC                                                                                    <  SRR19973063.63046/150‑1 (MQ=60)

                                                                  CATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGC                                                                                    >  SRR19973063.3135235/1‑150 (MQ=60)

                                                                  CATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGC                                                                                    <  SRR19973063.403491/150‑1 (MQ=60)

                                                                  CATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGC                                                                                    >  SRR19973063.202678/1‑150 (MQ=60)

                                                                  CATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATGTTGCATCGC                                                                                    >  SRR19973063.5104603/1‑150 (MQ=60)

                                                                    TGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCA                                                                                  <  SRR19973063.124366/150‑1 (MQ=60)

                                                                       CCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGAT                                                                               >  SRR19973063.2810370/1‑150 (MQ=60)

                                                                         ATACACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATAT                                                                             <  SRR19973063.1358583/150‑1 (MQ=60)

                                                                         ATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATAT                                                                             <  SRR19973063.1435258/150‑1 (MQ=60)

                                                                         ATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGACCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATAT                                                                             >  SRR19973063.4097203/1‑150 (MQ=60)

                                                                          TAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATA                                                                            >  SRR19973063.633193/1‑150 (MQ=60)

                                                                          TAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATA                                                                            <  SRR19973063.2718434/150‑1 (MQ=60)

                                                                           AAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAG                                                                           <  SRR19973063.2797357/150‑1 (MQ=60)

                                                                           AAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAG                                                                           <  SRR19973063.2797476/150‑1 (MQ=60)

                                                                            AACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGT                                                                          <  SRR19973063.2414034/150‑1 (MQ=60)

                                                                            AACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGT                                                                          >  SRR19973063.2751924/1‑150 (MQ=60)

                                                                             ACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTC                                                                         >  SRR19973063.551454/1‑150 (MQ=60)

                                                                             ACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTC                                                                         <  SRR19973063.314667/150‑1 (MQ=60)

                                                                              CAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCC                                                                        >  SRR19973063.1025814/1‑150 (MQ=60)

                                                                                AACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTG                                                                      <  SRR19973063.3723923/150‑1 (MQ=60)

                                                                                    CGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCA                                                                  <  SRR19973063.4084568/150‑1 (MQ=60)

                                                                                         CGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCAT                                                             >  SRR19973063.959431/1‑150 (MQ=60)

                                                                                          GCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATA                                                            >  SRR19973063.504891/1‑150 (MQ=60)

                                                                                            GCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAA                                                          <  SRR19973063.1034557/150‑1 (MQ=60)

                                                                                            GCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAA                                                          <  SRR19973063.3388962/150‑1 (MQ=60)

                                                                                                 AGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCC                                                     >  SRR19973063.815992/1‑150 (MQ=60)

                                                                                                 AGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCA                                                     <  SRR19973063.1574746/150‑1 (MQ=60)

                                                                                                  GACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCT                                                    <  SRR19973063.3834167/150‑1 (MQ=60)

                                                                                                   ACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTT                                                   <  SRR19973063.3509465/150‑1 (MQ=60)

                                                                                                       TTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCC                                               >  SRR19973063.4380364/1‑150 (MQ=60)

                                                                                                        TAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCT                                              <  SRR19973063.4892363/150‑1 (MQ=60)

                                                                                                         AGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTT                                             >  SRR19973063.1946561/1‑150 (MQ=60)

                                                                                                         AGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTT                                             <  SRR19973063.4454216/150‑1 (MQ=60)

                                                                                                         AGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTT                                             >  SRR19973063.1946530/1‑150 (MQ=60)

                                                                                                         AGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGACCTGGGCAACCATAAAAATCCTTTGCCTT                                             >  SRR19973063.856060/1‑150 (MQ=60)

                                                                                                           CGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCA                                           >  SRR19973063.2177165/1‑150 (MQ=60)

                                                                                                           CGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCA                                           >  SRR19973063.3987415/1‑150 (MQ=60)

                                                                                                              TCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGA                                        <  SRR19973063.3045507/150‑1 (MQ=60)

                                                                                                               CAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAG                                       <  SRR19973063.2340145/150‑1 (MQ=60)

                                                                                                               CAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAG                                       <  SRR19973063.4502399/150‑1 (MQ=60)

                                                                                                                AGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGA                                      >  SRR19973063.185549/1‑150 (MQ=60)

                                                                                                                  AAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATC                                    <  SRR19973063.3480793/150‑1 (MQ=60)

                                                                                                                   AGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCG                                   >  SRR19973063.2523989/1‑150 (MQ=60)

                                                                                                                    GATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGA                                  <  SRR19973063.4129426/150‑1 (MQ=60)

                                                                                                                       CTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTA                               <  SRR19973063.1713678/150‑1 (MQ=60)

                                                                                                                          CAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATTCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCT                            <  SRR19973063.4427816/150‑1 (MQ=60)

                                                                                                                          CAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCT                            <  SRR19973063.4427906/150‑1 (MQ=60)

                                                                                                                          CAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCT                            <  SRR19973063.4726059/150‑1 (MQ=60)

                                                                                                                           AATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGATTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTG                           <  SRR19973063.3303246/150‑1 (MQ=60)

                                                                                                                               TCGGAAGCTGAAATGTTACTTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCT                       <  SRR19973063.1572536/150‑1 (MQ=60)

                                                                                                                                CGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTT                      >  SRR19973063.992853/1‑150 (MQ=60)

                                                                                                                                CGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTT                      <  SRR19973063.4772830/150‑1 (MQ=60)

                                                                                                                                CGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTT                      >  SRR19973063.3227669/1‑150 (MQ=60)

                                                                                                                                 GGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTT                     <  SRR19973063.3116661/150‑1 (MQ=60)

                                                                                                                                  GAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTA                    <  SRR19973063.730938/150‑1 (MQ=60)

                                                                                                                                      CTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCA                >  SRR19973063.4877644/1‑150 (MQ=60)

                                                                                                                                      CTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCA                <  SRR19973063.2545292/150‑1 (MQ=60)

                                                                                                                                       TGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAA               >  SRR19973063.4729622/1‑150 (MQ=60)

                                                                                                                                       TGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAA               >  SRR19973063.3990816/1‑150 (MQ=60)

                                                                                                                                       TGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAA               >  SRR19973063.5164079/1‑150 (MQ=60)

                                                                                                                                       TGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAA               >  SRR19973063.5164106/1‑150 (MQ=60)

                                                                                                                                       TGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAA               >  SRR19973063.5164301/1‑150 (MQ=60)

                                                                                                                                       TGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGAACGAGTACCAGTTCTTAAGTCAA               >  SRR19973063.3990802/1‑150 (MQ=60)

                                                                                                                                        GAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAA              >  SRR19973063.4412861/1‑150 (MQ=60)

                                                                                                                                           ATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATGAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGA           <  SRR19973063.1961230/150‑1 (MQ=60)

                                                                                                                                           ATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGA           <  SRR19973063.4294638/150‑1 (MQ=60)

                                                                                                                                             GTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACA         <  SRR19973063.3318376/150‑1 (MQ=60)

                                                                                                                                             GTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACA         >  SRR19973063.3017473/1‑150 (MQ=60)

                                                                                                                                              TTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACAT        <  SRR19973063.3715874/150‑1 (MQ=60)

                                                                                                                                               TAATTTACTGGTCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATG       <  SRR19973063.1613798/150‑1 (MQ=60)

                                                                                                                                               TAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATG       <  SRR19973063.4209853/150‑1 (MQ=60)

                                                                                                                                                 ATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTG     >  SRR19973063.2671491/1‑150 (MQ=60)

                                                                                                                                                  TTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGC    <  SRR19973063.2726881/150‑1 (MQ=60)

                                                                                                                                                  TTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGC    >  SRR19973063.4767544/1‑150 (MQ=60)

                                                                                                                                                    TACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGA  >  SRR19973063.1194684/1‑150 (MQ=60)

                                                                                                                                                    TACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGA  >  SRR19973063.1151422/1‑150 (MQ=60)

AAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGA  >  CP009273/4210604‑4210901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`