Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,348,772 G→T 100% P2T (CCT→ACT) 
A319D (GCC→GAC) 		 sapC ←
sapB ←		 antimicrobial peptide transport ABC transporter permease
antimicrobial peptide transport ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,348,7720GT99.1% 759.1 / ‑2.1 236P2T (CCT→ACT) 
A319D (GCC→GAC) 		sapC
sapB		antimicrobial peptide transport ABC transporter permease
antimicrobial peptide transport ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base T (96/137);  total (96/140)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 236 total aligned reads displayed.

GCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACC  >  CP009273/1348627‑1348916
                                                                                                                                                 |                                                                                                                                                
gcAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCa                                                                                                                                                <  1:372238/148‑1 (MQ=255)
 cAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCAt                                                                                                                                               <  2:3440418/148‑1 (MQ=255)
   aaCCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATAc                                                                                                                                             <  2:3017872/148‑1 (MQ=255)
      cAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCAt                                                                                                                                          <  2:2869444/148‑1 (MQ=255)
       aGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCAtt                                                                                                                                         <  1:13548/148‑1 (MQ=255)
       aGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCAtt                                                                                                                                         >  2:480050/1‑148 (MQ=255)
       aGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCAtt                                                                                                                                         <  2:396811/148‑1 (MQ=255)
        gccgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTc                                                                                                                                        <  1:1423131/148‑1 (MQ=255)
        gccgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTc                                                                                                                                        <  1:3144911/148‑1 (MQ=255)
        gccgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTc                                                                                                                                        >  2:3063759/1‑148 (MQ=255)
         ccgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTcc                                                                                                                                       >  2:1893435/1‑148 (MQ=255)
         ccgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTcc                                                                                                                                       >  1:2801210/1‑148 (MQ=255)
         ccgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTcc                                                                                                                                       <  1:1512200/148‑1 (MQ=255)
         ccgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTcc                                                                                                                                       >  1:1878890/1‑148 (MQ=255)
         ccgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTcc                                                                                                                                       <  1:3203081/148‑1 (MQ=255)
          cgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCt                                                                                                                                      <  2:1043518/148‑1 (MQ=255)
           gccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACAATTCCtt                                                                                                                                     <  2:2705651/148‑1 (MQ=255)
            ccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCCGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTa                                                                                                                                    >  2:325691/1‑148 (MQ=255)
            ccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTa                                                                                                                                    <  1:2799885/148‑1 (MQ=255)
            ccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTa                                                                                                                                    >  2:2094921/1‑148 (MQ=255)
            ccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTa                                                                                                                                    <  2:1302639/148‑1 (MQ=255)
             cAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTAt                                                                                                                                   >  1:3217118/1‑148 (MQ=255)
              aaaaaTACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATg                                                                                                                                  <  2:617302/148‑1 (MQ=255)
                aaaTACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGtt                                                                                                                                <  2:2914688/148‑1 (MQ=255)
                 aaTACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGttt                                                                                                                               <  1:3176564/148‑1 (MQ=255)
                  aTACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTc                                                                                                                              <  1:1058462/148‑1 (MQ=255)
                     cacaGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCCGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCaga                                                                                                                           >  2:1945821/1‑148 (MQ=255)
                          tACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGtt                                                                                                                      >  2:1633015/1‑148 (MQ=255)
                          tACAGCCAGCCCCGCGCAGCCGTACAGGCAGACCATCGCTGAGTCGTAACTATAAAATTTGCGCCAGGCGGTACTCAGCGTGCCCGGCGTGCGCTTTTCGCTGTATACTCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGtt                                                                                                                      <  2:687554/148‑1 (MQ=255)
                           aCAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTa                                                                                                                     <  2:779877/148‑1 (MQ=255)
                                 agccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATg                                                                                                               <  2:1819227/148‑1 (MQ=255)
                                  gccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATgg                                                                                                              >  2:2852310/1‑148 (MQ=255)
                                  gccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCGATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGGTAGCCCTgg                                                                                                              >  1:358459/1‑148 (MQ=255)
                                   ccccGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGc                                                                                                             <  1:1133632/148‑1 (MQ=255)
                                      cgcgCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGttt                                                                                                                               >  2:2472177/1‑127 (MQ=255)
                                      cgcgCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGttt                                                                                                                               <  1:2472177/127‑1 (MQ=255)
                                      cgcgCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCAcc                                                                                                          <  2:2221295/148‑1 (MQ=255)
                                          cAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACACaaa                                                                                                      >  2:1005961/1‑148 (MQ=255)
                                            gCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCCTACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAat                                                                                                    >  1:1915093/1‑148 (MQ=255)
                                                 aCAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAg                                                                                               <  2:3473352/148‑1 (MQ=255)
                                                    ggCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGaaa                                                                                            >  2:875658/1‑148 (MQ=255)
                                                     gCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAAt                                                                                           <  2:1610994/148‑1 (MQ=255)
                                                      ccGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATc                                                                                          >  1:2631405/1‑148 (MQ=255)
                                                       cGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCa                                                                                         <  2:87623/148‑1 (MQ=255)
                                                          ccATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTGGCTGTATAAGCTATCGTAAGTCATAACAATCCTTATGTTTCAGAGGGTTAGCCAAGGCACCCAAAAAATAAGAAAAAACGt                                                                                      >  1:45586/1‑148 (MQ=255)
                                                          ccATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTGGCTGTATAAGCTATAGTAATTCATACCAATCCTTATTTTTCAGAGGGTTAGCCAAGGCACCCAAAATATAAGAAAAAACGt                                                                                      >  1:45638/1‑148 (MQ=255)
                                                          ccATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGt                                                                                      >  1:810206/1‑148 (MQ=255)
                                                            aTCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTa                                                                                    <  1:2497128/148‑1 (MQ=255)
                                                             tCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTaa                                                                                   <  2:2652785/148‑1 (MQ=255)
                                                              cGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTGGCTGTATACGCGATCGTAAGTCATAACAATACGTATGTTTCAGATGGTTAGCCATGGCCCCCAAAATAAAAGAAATAACGGTAAc                                                                                  >  1:935257/1‑148 (MQ=255)
                                                              cGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAAc                                                                                  >  2:3286343/1‑148 (MQ=255)
                                                               gCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACa                                                                                 <  1:760071/148‑1 (MQ=255)
                                                                  gagGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACaata                                                                              >  1:2342499/1‑148 (MQ=255)
                                                                    ggCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACaataat                                                                            <  1:1087369/148‑1 (MQ=255)
                                                                       gTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGAc                                                                         <  1:1811751/148‑1 (MQ=255)
                                                                        tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCCTTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGAcc                                                                        >  2:3294390/1‑148 (MQ=255)
                                                                        tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGAcc                                                                        <  1:1060667/148‑1 (MQ=255)
                                                                        tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGAcc                                                                        <  2:2276648/148‑1 (MQ=255)
                                                                        tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGAcc                                                                        <  1:325945/148‑1 (MQ=255)
                                                                            tataAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTg                                                                    <  2:1927600/148‑1 (MQ=255)
                                                                            tataAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTg                                                                    >  2:1519730/1‑148 (MQ=255)
                                                                            tataAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTg                                                                    <  2:1901605/148‑1 (MQ=255)
                                                                            tataAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGATTTTCGATGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAAAAATTACCAGTg                                                                    >  2:112463/1‑148 (MQ=255)
                                                                               aaaaTTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGc                                                                 >  2:508981/1‑148 (MQ=255)
                                                                                aaaTTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGcc                                                                <  2:1863582/148‑1 (MQ=255)
                                                                                  aTTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCcac                                                              >  1:1450148/1‑148 (MQ=255)
                                                                                    ttGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTGGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCcacac                                                            >  1:978172/1‑148 (MQ=255)
                                                                                    ttGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCcacac                                                            >  1:2890886/1‑148 (MQ=255)
                                                                                         ccAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCAt                                                       <  2:3171453/148‑1 (MQ=255)
                                                                                         ccAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCAt                                                       <  1:3297687/148‑1 (MQ=255)
                                                                                          cAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATc                                                      <  2:439992/148‑1 (MQ=255)
                                                                                           aGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCCAAATATCAGAAATCACGTTAACAAAAATTACCAGTGAGCCACACACCATCa                                                     >  2:1197090/1‑148 (MQ=255)
                                                                                            ggCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCAc                                                    >  2:427306/1‑148 (MQ=255)
                                                                                              cGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTc                                                  <  1:2623224/148‑1 (MQ=255)
                                                                                                gTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCcg                                                <  1:2057873/148‑1 (MQ=255)
                                                                                                     cAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGa                                           >  1:3440457/1‑148 (MQ=255)
                                                                                                      aGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGaa                                          <  1:855225/148‑1 (MQ=255)
                                                                                                      aGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGaa                                          <  1:2793800/148‑1 (MQ=255)
                                                                                                       gCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGaaa                                         <  1:2452626/148‑1 (MQ=255)
                                                                                                       gCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGaaa                                         <  1:684062/148‑1 (MQ=255)
                                                                                                           gCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGc                                     <  2:3108179/148‑1 (MQ=255)
                                                                                                                  gggcgCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTaa                              <  1:2751023/148‑1 (MQ=255)
                                                                                                                  gggcgCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTaa                              <  1:997483/148‑1 (MQ=255)
                                                                                                                   ggcgCTTTTCGCTGTATACGCTATCGTAAGTCATAACATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCAACACTCCGGCGGAAATGGCTGCGTAAt                             >  1:487856/1‑148 (MQ=255)
                                                                                                                    gcgcTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATc                            >  2:1389829/1‑148 (MQ=255)
                                                                                                                    gcgcTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATc                            >  2:3292785/1‑148 (MQ=255)
                                                                                                                     cgcTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATcc                           <  1:2248609/148‑1 (MQ=255)
                                                                                                                     cgcTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATcc                           >  1:650012/1‑148 (MQ=255)
                                                                                                                     cgcTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATcc                           <  1:2641370/148‑1 (MQ=255)
                                                                                                                     cgcTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATcc                           <  2:45586/148‑1 (MQ=255)
                                                                                                                       cTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCctg                         >  2:109005/1‑148 (MQ=255)
                                                                                                                        ttttCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCctgc                        >  1:1582959/1‑148 (MQ=255)
                                                                                                                         tttCGCGGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCctgct                       <  2:2620167/148‑1 (MQ=255)
                                                                                                                          ttCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCctgctg                      <  1:1040378/148‑1 (MQ=255)
                                                                                                                                   taCGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGc             <  1:875669/148‑1 (MQ=255)
                                                                                                                                     cGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCCCGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGt           >  2:1041651/1‑148 (MQ=255)
                                                                                                                                        tATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGttaa        <  2:2041934/148‑1 (MQ=255)
                                                                                                                                             tAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACGCCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAAc   >  1:1799045/1‑148 (MQ=255)
                                                                                                                                             tAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAAc   >  2:11756/1‑148 (MQ=255)
                                                                                                                                              aaGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAAcc  >  2:2033431/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACC  >  CP009273/1348627‑1348916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGC  >  CP009273/1348623‑1348919
                                                                                                                                                     |                                                                                                                                                   
AGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGT                                                                                                                                                     <  SRR19973065.638217/150‑1 (MQ=60)
AGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATAAGCTATCGTAAGT                                                                                                                                                     >  SRR19973065.2039546/1‑150 (MQ=60)
    GCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATA                                                                                                                                                 <  SRR19973065.373841/150‑1 (MQ=60)
    GCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATA                                                                                                                                                 <  SRR19973065.374841/150‑1 (MQ=60)
           AGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCC                                                                                                                                          >  SRR19973065.1891260/1‑150 (MQ=60)
           AGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCC                                                                                                                                          <  SRR19973065.13753/150‑1 (MQ=60)
           AGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCC                                                                                                                                          >  SRR19973065.2818554/1‑150 (MQ=60)
           AGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCC                                                                                                                                          >  SRR19973065.2818560/1‑150 (MQ=60)
           AGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCC                                                                                                                                          >  SRR19973065.105004/1‑150 (MQ=60)
            GCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCT                                                                                                                                         <  SRR19973065.3164014/150‑1 (MQ=60)
            GCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCT                                                                                                                                         <  SRR19973065.3164659/150‑1 (MQ=60)
            GCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCT                                                                                                                                         <  SRR19973065.1432408/150‑1 (MQ=60)
             CCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTT                                                                                                                                        <  SRR19973065.3224048/150‑1 (MQ=60)
             CCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTT                                                                                                                                        <  SRR19973065.459291/150‑1 (MQ=60)
             CCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTT                                                                                                                                        <  SRR19973065.1764370/150‑1 (MQ=60)
             CCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTT                                                                                                                                        <  SRR19973065.3222365/150‑1 (MQ=60)
             CCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTT                                                                                                                                        <  SRR19973065.1522059/150‑1 (MQ=60)
             CCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCNT                                                                                                                                        <  SRR19973065.843486/150‑1 (MQ=60)
               GCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTAT                                                                                                                                      >  SRR19973065.408505/1‑150 (MQ=60)
               GCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTAT                                                                                                                                      >  SRR19973065.3236464/1‑150 (MQ=60)
                CCAAAAATACACAGTGCAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATG                                                                                                                                     <  SRR19973065.2817223/150‑1 (MQ=60)
                CCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATG                                                                                                                                     <  SRR19973065.2817229/150‑1 (MQ=60)
                CCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATG                                                                                                                                     <  SRR19973065.58520/150‑1 (MQ=60)
                CCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATG                                                                                                                                     <  SRR19973065.2817197/150‑1 (MQ=60)
                     acgtgtgctcttccgatctCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCA                                                                                                                                <  SRR19973065.2487741/131‑1 (MQ=60)
                     AATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCA                                                                                                                                <  SRR19973065.3196402/150‑1 (MQ=60)
                     AATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCA                                                                                                                                <  SRR19973065.3195763/150‑1 (MQ=60)
                      ATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAG                                                                                                                               <  SRR19973065.1065480/150‑1 (MQ=60)
                        ACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAG                                                                                                                             <  SRR19973065.1124540/150‑1 (MQ=60)
                        ACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCGTTTCGCTGGATACGCTATCGTAAGTCATACCAATCCTTATGTTTCAGAG                                                                                                                             >  SRR19973065.3381900/1‑150 (MQ=60)
                         CACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGG                                                                                                                            <  SRR19973065.2182467/150‑1 (MQ=60)
                          ACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGG                                                                                                                           <  SRR19973065.1823931/150‑1 (MQ=60)
                           CAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGT                                                                                                                          <  SRR19973065.10806/150‑1 (MQ=60)
                           CAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGT                                                                                                                          <  SRR19973065.10751/150‑1 (MQ=60)
                              TACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAG                                                                                                                       >  SRR19973065.2765346/1‑150 (MQ=60)
                                    CAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGG                                                                                                                 <  SRR19973065.1967637/150‑1 (MQ=60)
                                    CAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCGATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGGTAGCCCTGG                                                                                                                 >  SRR19973065.360983/1‑150 (MQ=60)
                                     AGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGC                                                                                                                >  SRR19973065.2697887/1‑150 (MQ=60)
                                     AGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGC                                                                                                                <  SRR19973065.1552606/150‑1 (MQ=60)
                                       CCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCAC                                                                                                              <  SRR19973065.3490674/150‑1 (MQ=60)
                                       CCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCAC                                                                                                              <  SRR19973065.1140984/150‑1 (MQ=60)
                                              CAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCCTACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAAT                                                                                                       >  SRR19973065.1927659/1‑150 (MQ=60)
                                              CAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAAT                                                                                                       <  SRR19973065.2343058/150‑1 (MQ=60)
                                                GCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATAT                                                                                                     <  SRR19973065.2543016/150‑1 (MQ=60)
                                                  CGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATAGTAAGTCATACCATTCCATATGTTTCAGAGGGTTAGCCAAGGCACCCAAAAAATCA                                                                                                   >  SRR19973065.1850143/1‑150 (MQ=60)
                                                        GGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATC                                                                                             >  SRR19973065.2647670/1‑150 (MQ=60)
                                                          CCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCAC                                                                                           <  SRR19973065.2869847/150‑1 (MQ=60)
                                                            GACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTGGCTGTATAAGCTATCGTAAGTCATAACAATCCTTATGTTTCAGAGGGTTAGCCAAGGCACCCAAAAaataagaaaaaacgt                                                                                         >  SRR19973065.46122/1‑135 (MQ=60)
                                                            GACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTGGCTGTATAAGCTATAGTAATTCATACCAATCCTTATTTTTCAGAGGGTTAGCCAAGGCACCCAAAATATAAGAAAAAACGT                                                                                         >  SRR19973065.46174/1‑150 (MQ=60)
                                                            GACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGT                                                                                         >  SRR19973065.1489092/1‑150 (MQ=60)
                                                            GACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGT                                                                                         >  SRR19973065.2034096/1‑150 (MQ=60)
                                                            GACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGT                                                                                         >  SRR19973065.815552/1‑150 (MQ=60)
                                                             ACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTT                                                                                        <  SRR19973065.3450192/150‑1 (MQ=60)
                                                                ATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTGGCTGTATACGCGATCGTAAGTCATAACAATACGTATGTTTCAGATGGTTAGCCATGGCCCCCAAAATAAAAGAAATAACGGTAAC                                                                                     >  SRR19973065.941432/1‑150 (MQ=60)
                                                                ATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAAC                                                                                     <  SRR19973065.2512839/150‑1 (MQ=60)
                                                                 TCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACA                                                                                    <  SRR19973065.1419299/150‑1 (MQ=60)
                                                                 TCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACA                                                                                    <  SRR19973065.1419290/150‑1 (MQ=60)
                                                                   GCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAAT                                                                                  <  SRR19973065.2496397/150‑1 (MQ=60)
                                                                   GCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAAT                                                                                  <  SRR19973065.765135/150‑1 (MQ=60)
                                                                   GCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAAT                                                                                  <  SRR19973065.765157/150‑1 (MQ=60)
                                                                   GCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTAGCTGTATAAGCGATCGTAAGTCATAACATTCCGTATTTTTAAAAGGGTTAGCCAATGCCCCCAAAATATAAGAAATCACGGTAACAAT                                                                                  >  SRR19973065.2973921/1‑150 (MQ=60)
                                                                    CAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATA                                                                                 >  SRR19973065.2357120/1‑150 (MQ=60)
                                                                    CAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATA                                                                                 <  SRR19973065.939560/150‑1 (MQ=60)
                                                                    CAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATA                                                                                 >  SRR19973065.2357127/1‑150 (MQ=60)
                                                                      GAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAAT                                                                               <  SRR19973065.3251626/150‑1 (MQ=60)
                                                                        GGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGA                                                                             <  SRR19973065.1094536/150‑1 (MQ=60)
                                                                           GTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCA                                                                          <  SRR19973065.1823671/150‑1 (MQ=60)
                                                                           GTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCA                                                                          <  SRR19973065.1908142/150‑1 (MQ=60)
                                                                            TCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAG                                                                         <  SRR19973065.1068969/150‑1 (MQ=60)
                                                                            TCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAG                                                                         <  SRR19973065.1067698/150‑1 (MQ=60)
                                                                            TCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAG                                                                         <  SRR19973065.328213/150‑1 (MQ=60)
                                                                              ACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCGTTTAGCTGTATACGCAATCGTAAGTCATAACATTCCTTATGTTTGAGAGGGTTAGCCATGGCACCCAAAATAACAGAAATCACGTTAACAATAATGACCACTG                                                                       >  SRR19973065.972828/1‑150 (MQ=60)
                                                                                 ATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGC                                                                    <  SRR19973065.994005/150‑1 (MQ=60)
                                                                                   AAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCA                                                                  <  SRR19973065.1584919/150‑1 (MQ=60)
                                                                                    AAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCAC                                                                 >  SRR19973065.1459355/1‑150 (MQ=60)
                                                                                    AAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATAACGTTAACAATAATTACCAGTGAGCCAC                                                                 >  SRR19973065.1459509/1‑150 (MQ=60)
                                                                                     AATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATTACCAGTGAGCCACA                                                                >  SRR19973065.1000011/1‑150 (MQ=60)
                                                                                      ATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTGGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACAC                                                               >  SRR19973065.984664/1‑150 (MQ=60)
                                                                                      ATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACAC                                                               >  SRR19973065.2908551/1‑150 (MQ=60)
                                                                                             CCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCA                                                        <  SRR19973065.3317769/150‑1 (MQ=60)
                                                                                                  CGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCG                                                   <  SRR19973065.2639597/150‑1 (MQ=60)
                                                                                                  CGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCG                                                   <  SRR19973065.2639458/150‑1 (MQ=60)
                                                                                                   GGTACGCAGCGTGCCCGGCGGGCGCTTTTGGCTGTATAAGCTATCGTAAGTCATACCAATCCGTATTTTTCAGAGGGGTATCCATGGCACCCAAAATATAAGAAAACACGTTAACAATAAAGACCAGTGAGCCACACACCAaaactcccg                                                  >  SRR19973065.3256179/1‑141 (MQ=60)
                                                                                                    GTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGC                                                 <  SRR19973065.2071011/150‑1 (MQ=60)
                                                                                                       CGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGA                                              >  SRR19973065.3461440/1‑150 (MQ=60)
                                                                                                          AGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAAT                                           <  SRR19973065.860754/150‑1 (MQ=60)
                                                                                                          AGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAAT                                           <  SRR19973065.2811113/150‑1 (MQ=60)
                                                                                                           GCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATG                                          <  SRR19973065.1257414/150‑1 (MQ=60)
                                                                                                           GCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATG                                          <  SRR19973065.2468082/150‑1 (MQ=60)
                                                                                                           GCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATG                                          <  SRR19973065.688611/150‑1 (MQ=60)
                                                                                                           GCGTGCCCGGCGGGCGCTTTTAGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATTGCACCCAAAATATCAGAAATCACGTTAACAATAATTACCAGTGAGCCACACACCATCACTCCGGCGGAAATG                                          <  SRR19973065.2468135/150‑1 (MQ=60)
                                                                                                                   GGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTA                                  <  SRR19973065.2544106/150‑1 (MQ=60)
                                                                                                                     CGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATAACATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCAACACTCCGGCGGAAATGGCTGCGTAAT                                >  SRR19973065.491155/1‑150 (MQ=60)
                                                                                                                      GGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATC                               <  SRR19973065.1004111/150‑1 (MQ=60)
                                                                                                                      GGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATC                               <  SRR19973065.2768223/150‑1 (MQ=60)
                                                                                                                      GGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATC                               <  SRR19973065.2768139/150‑1 (MQ=60)
                                                                                                                       GGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCC                              >  SRR19973065.654291/1‑150 (MQ=60)
                                                                                                                       GGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCC                              >  SRR19973065.654300/1‑150 (MQ=60)
                                                                                                                         CGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTG                            <  SRR19973065.483484/150‑1 (MQ=60)
                                                                                                                         CGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTG                            <  SRR19973065.2657676/150‑1 (MQ=60)
                                                                                                                         CGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTG                            <  SRR19973065.2262869/150‑1 (MQ=60)
                                                                                                                          GCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGC                           >  SRR19973065.1593517/1‑150 (MQ=60)
                                                                                                                          GCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGC                           >  SRR19973065.2390930/1‑150 (MQ=60)
                                                                                                                           CTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCT                          >  SRR19973065.3234260/1‑150 (MQ=60)
                                                                                                                              TTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGC                       <  SRR19973065.1047266/150‑1 (MQ=60)
                                                                                                                                 GCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGA                    >  SRR19973065.691514/1‑150 (MQ=60)
                                                                                                                                 GCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGGAATCCTGCTGGCGGA                    >  SRR19973065.691631/1‑150 (MQ=60)
                                                                                                                                   TGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATG                  <  SRR19973065.483317/150‑1 (MQ=60)
                                                                                                                                   TGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATG                  >  SRR19973065.1811687/1‑150 (MQ=60)
                                                                                                                                       TACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGT              <  SRR19973065.881245/150‑1 (MQ=60)
                                                                                                                                       TACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGT              <  SRR19973065.881251/150‑1 (MQ=60)
                                                                                                                                       TACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGT              <  SRR19973065.881262/150‑1 (MQ=60)
                                                                                                                                       TACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGT              <  SRR19973065.2649429/150‑1 (MQ=60)
                                                                                                                                          GCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAA           >  SRR19973065.3303042/1‑150 (MQ=60)
                                                                                                                                           CTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAAT          <  SRR19973065.2914250/150‑1 (MQ=60)
                                                                                                                                               CGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACGCCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAAC      >  SRR19973065.1810857/1‑150 (MQ=60)
                                                                                                                                               CGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAAC      <  SRR19973065.447492/150‑1 (MQ=60)
                                                                                                                                                 TAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCA    >  SRR19973065.293788/1‑150 (MQ=60)
                                                                                                                                                 TAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCA    >  SRR19973065.293246/1‑150 (MQ=60)
                                                                                                                                                  AAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGACATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAG   >  SRR19973065.3235898/1‑150 (MQ=60)
                                                                                                                                                  AAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAG   >  SRR19973065.1667595/1‑150 (MQ=60)
                                                                                                                                                   AGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGC  >  SRR19973065.2500138/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
AGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGC  >  CP009273/1348623‑1348919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑