Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,348,772 G→T 100% P2T (CCT→ACT) 
A319D (GCC→GAC) 		 sapC ←
sapB ←		 antimicrobial peptide transport ABC transporter permease
antimicrobial peptide transport ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,348,7720GT99.5% 599.8 / ‑4.0 184P2T (CCT→ACT) 
A319D (GCC→GAC) 		sapC
sapB		antimicrobial peptide transport ABC transporter permease
antimicrobial peptide transport ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (61/122);  total (61/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 184 total aligned reads displayed.

AAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACC  >  CP009273/1348629‑1348916
                                                                                                                                               |                                                                                                                                                
  aCCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATAcc                                                                                                                                            >  2:1731713/1‑148 (MQ=255)
     aGCCGCCAAAAAGACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATACAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCAtt                                                                                                                                         <  2:2643598/148‑1 (MQ=255)
      gccgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTc                                                                                                                                        >  2:1613453/1‑148 (MQ=255)
      gccgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTc                                                                                                                                        <  1:2501626/148‑1 (MQ=255)
      gccgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTc                                                                                                                                        <  1:1788320/148‑1 (MQ=255)
       ccgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTcc                                                                                                                                       <  2:3095114/148‑1 (MQ=255)
       ccgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTcc                                                                                                                                       <  2:3095142/148‑1 (MQ=255)
       ccgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTcc                                                                                                                                       <  2:3795398/148‑1 (MQ=255)
        cgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCt                                                                                                                                      <  1:3789409/148‑1 (MQ=255)
        cgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCt                                                                                                                                      <  1:1879474/148‑1 (MQ=255)
         gccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCtt                                                                                                                                     <  2:449534/148‑1 (MQ=255)
         gccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCtt                                                                                                                                     <  1:145519/148‑1 (MQ=255)
         gccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCtt                                                                                                                                     <  2:2063222/148‑1 (MQ=255)
          ccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTa                                                                                                                                    >  2:2274436/1‑148 (MQ=255)
             aaaaTACACAGTACAGCCAGCCCCTCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGt                                                                                                                                 <  2:2547733/148‑1 (MQ=255)
                aTACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTc                                                                                                                              >  1:308900/1‑148 (MQ=255)
                aTACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTc                                                                                                                              <  1:1554931/148‑1 (MQ=255)
                aTACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTc                                                                                                                              <  1:3453177/148‑1 (MQ=255)
                  acacAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCag                                                                                                                            >  1:3188522/1‑148 (MQ=255)
                  acacAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCag                                                                                                                            >  1:1363285/1‑148 (MQ=255)
                   cacaGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCCTTCCTGATGTTTCaga                                                                                                                           >  2:2709342/1‑148 (MQ=255)
                   cacaGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCaga                                                                                                                           <  1:2521079/148‑1 (MQ=255)
                   cacaGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCGTATGTTTCaga                                                                                                                           >  1:2632061/1‑148 (MQ=255)
                   cacaGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCGATCGTAAGTCATACCATTCCTGATGTTTCaga                                                                                                                           >  2:2875907/1‑148 (MQ=255)
                    acaGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCagag                                                                                                                          <  1:485976/148‑1 (MQ=255)
                    acaGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCagag                                                                                                                          <  2:254587/148‑1 (MQ=255)
                    acaGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCagag                                                                                                                          <  1:485899/148‑1 (MQ=255)
                         aCAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTa                                                                                                                     <  2:691544/148‑1 (MQ=255)
                           agccagccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCaga                                                                                                                           <  1:3572640/140‑1 (MQ=255)
                            gccagccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGcc                                                                                                                  <  2:1882202/148‑1 (MQ=255)
                            gccagccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGcc                                                                                                                  <  2:977450/148‑1 (MQ=255)
                             ccagccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCa                                                                                                                 <  2:1580125/148‑1 (MQ=255)
                             ccagccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCa                                                                                                                 <  1:220556/148‑1 (MQ=255)
                                gccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATgg                                                                                                              <  2:1189590/148‑1 (MQ=255)
                                gccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATgg                                                                                                              >  2:227324/1‑148 (MQ=255)
                                gccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATgg                                                                                                              <  2:1189016/148‑1 (MQ=255)
                                 ccccGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATAAGCTATCGGAAGTCATACCAATCCTTATGTTTCAGAGGGTTAGCCAAGGc                                                                                                             >  1:34683/1‑148 (MQ=255)
                                    cgcgCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCAcc                                                                                                          <  1:2419336/148‑1 (MQ=255)
                                        cAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCaaa                                                                                                      <  1:312346/148‑1 (MQ=255)
                                          gCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCGTATGTTTAAGAGGGTTAGCCAAGGCACCCaaaaa                                                                                                    >  1:2605268/1‑147 (MQ=255)
                                           ccGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAata                                                                                                   >  2:2944484/1‑148 (MQ=255)
                                                 aGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGaa                                                                                             >  2:348980/1‑148 (MQ=255)
                                                   gCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAAt                                                                                           >  2:290047/1‑148 (MQ=255)
                                                    ccGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATc                                                                                          <  1:973708/148‑1 (MQ=255)
                                                       aCCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACg                                                                                       <  1:106563/148‑1 (MQ=255)
                                                       aCCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCAGTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACg                                                                                       >  1:820670/1‑148 (MQ=255)
                                                          aTCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGACATCACGTTa                                                                                    >  2:578372/1‑148 (MQ=255)
                                                          aTCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTa                                                                                    <  1:1817263/148‑1 (MQ=255)
                                                           tCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTaa                                                                                   >  1:2735503/1‑148 (MQ=255)
                                                           tCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCGATCGTAAGTCCAACCATTCCTTATGTTTCAGAGGGTTAGCCCTGGCACCCCAAATATCAGAAAACACGTTaa                                                                                   >  1:376516/1‑148 (MQ=255)
                                                              cAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACaa                                                                                <  2:3454776/148‑1 (MQ=255)
                                                                gagGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCTTACGTTTTGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACaata                                                                              <  1:3747094/148‑1 (MQ=255)
                                                                gagGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACaata                                                                              <  2:660321/148‑1 (MQ=255)
                                                                gagGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACaata                                                                              <  1:3475991/148‑1 (MQ=255)
                                                                 agGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACaataa                                                                             >  2:303413/1‑148 (MQ=255)
                                                                 agGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACaataa                                                                             <  2:2871910/148‑1 (MQ=255)
                                                                 agGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACaataa                                                                             <  1:1277899/148‑1 (MQ=255)
                                                                 agGCGTCACTATAAAATTTGCGCCAGGCAGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACaataa                                                                             <  1:691653/148‑1 (MQ=255)
                                                                   gCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCGTATGTGTCAGAGGGTTAGCCATGGCACCCACAATATCAGAAATCACGTTAACAATAATg                                                                           >  2:3303969/1‑148 (MQ=255)
                                                                      tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGAcc                                                                        <  2:491234/148‑1 (MQ=255)
                                                                      tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATAAGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGAcc                                                                        <  2:1244712/148‑1 (MQ=255)
                                                                          tataAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTg                                                                    >  1:2804785/1‑148 (MQ=255)
                                                                          tataAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTg                                                                    >  1:889628/1‑148 (MQ=255)
                                                                            taAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCAATCCTTATGTTTCAGAGGGTTAGCCAAGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAg                                                                  >  1:2966897/1‑148 (MQ=255)
                                                                               aaTTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCca                                                               <  1:3283526/148‑1 (MQ=255)
                                                                               aaTTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCca                                                               <  1:3283651/148‑1 (MQ=255)
                                                                               aaTTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCca                                                               <  2:356056/148‑1 (MQ=255)
                                                                                aTTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCcac                                                              >  2:594886/1‑148 (MQ=255)
                                                                                  ttGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCcacac                                                            <  2:3235020/148‑1 (MQ=255)
                                                                                   tGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCcacaca                                                           >  1:1678102/1‑148 (MQ=255)
                                                                                         aGGCTGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATAGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCTCACACCATCa                                                     <  2:2622498/148‑1 (MQ=255)
                                                                                          ggCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCAc                                                    >  2:3621096/1‑148 (MQ=255)
                                                                                            cGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTc                                                  <  1:3418261/148‑1 (MQ=255)
                                                                                               tACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCcgg                                               <  1:3468849/148‑1 (MQ=255)
                                                                                               tACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCcgg                                               >  2:3599440/1‑148 (MQ=255)
                                                                                               tACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGGTAACAATAATGACCAGTGAGCCACACACCATCACTCcgg                                               >  1:1819344/1‑148 (MQ=255)
                                                                                                    aGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCATTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACACTAATGACCAGTGAGCCACACACCATCACTCCGGCGGaa                                          <  1:2896650/148‑1 (MQ=255)
                                                                                                      cGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAAt                                        <  2:1149175/148‑1 (MQ=255)
                                                                                                      cGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAAt                                        >  2:1519947/1‑148 (MQ=255)
                                                                                                      cGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAAt                                        <  1:385876/148‑1 (MQ=255)
                                                                                                        tGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATgg                                      <  2:3763842/148‑1 (MQ=255)
                                                                                                          cccGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCt                                    <  2:820670/148‑1 (MQ=255)
                                                                                                               cgggcgCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTa                               <  1:3636223/148‑1 (MQ=255)
                                                                                                                gggcgCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTaa                              <  2:2804785/148‑1 (MQ=255)
                                                                                                                gggcgCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTaa                              <  2:889628/148‑1 (MQ=255)
                                                                                                                 ggcgCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCGAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAAt                             <  2:1060088/148‑1 (MQ=255)
                                                                                                                 ggcgCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAAt                             <  1:2128401/148‑1 (MQ=255)
                                                                                                                  gcgcTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATc                            <  1:2228114/148‑1 (MQ=255)
                                                                                                                  gcgcTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATc                            <  1:2001680/148‑1 (MQ=255)
                                                                                                                     cTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCctg                         <  1:658881/148‑1 (MQ=255)
                                                                                                                     cTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCctg                         >  2:2383462/1‑148 (MQ=255)
                                                                                                                        ttCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGGACTCctgctg                      >  1:2281217/1‑148 (MQ=255)
                                                                                                                            cTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCgg                  >  2:876636/1‑148 (MQ=255)
                                                                                                                             tGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGa                 <  1:2859427/148‑1 (MQ=255)
                                                                                                                                      tATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGttaa        <  2:863553/148‑1 (MQ=255)
                                                                                                                                       aTCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGttaat       >  2:1969040/1‑148 (MQ=255)
                                                                                                                                         cGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCCTCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGttaatta     >  2:2439295/1‑148 (MQ=255)
                                                                                                                                         cGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGttaatta     >  2:517968/1‑148 (MQ=255)
                                                                                                                                          gTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGttaattaa    <  2:1664066/148‑1 (MQ=255)
                                                                                                                                            aaGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAAcc  <  2:1363285/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
AAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACC  >  CP009273/1348629‑1348916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCG  >  CP009273/1348623‑1348920
                                                                                                                                                     |                                                                                                                                                    
AGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGT                                                                                                                                                      <  SRR19973069.1149864/150‑1 (MQ=60)
            GCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCT                                                                                                                                          <  SRR19973069.2518329/150‑1 (MQ=60)
            GCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCT                                                                                                                                          <  SRR19973069.1800916/150‑1 (MQ=60)
             CCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTT                                                                                                                                         <  SRR19973069.2166720/150‑1 (MQ=60)
             CCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTT                                                                                                                                         <  SRR19973069.1466172/150‑1 (MQ=60)
              CGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTA                                                                                                                                        <  SRR19973069.1892540/150‑1 (MQ=60)
              CGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTA                                                                                                                                        <  SRR19973069.3813984/150‑1 (MQ=60)
               GCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTAT                                                                                                                                       <  SRR19973069.146825/150‑1 (MQ=60)
               GCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTAT                                                                                                                                       <  SRR19973069.2861338/150‑1 (MQ=60)
                CCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATG                                                                                                                                      <  SRR19973069.3818512/150‑1 (MQ=60)
                    AAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTC                                                                                                                                  >  SRR19973069.310973/1‑150 (MQ=60)
                      ATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAG                                                                                                                                <  SRR19973069.1565557/150‑1 (MQ=60)
                      ATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAG                                                                                                                                >  SRR19973069.3209231/1‑150 (MQ=60)
                      ATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAG                                                                                                                                >  SRR19973069.1372766/1‑150 (MQ=60)
                      ATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAG                                                                                                                                >  SRR19973069.3286288/1‑150 (MQ=60)
                      ATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAG                                                                                                                                <  SRR19973069.3475561/150‑1 (MQ=60)
                       TACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCGTATGTTTCAGA                                                                                                                               >  SRR19973069.2649688/1‑150 (MQ=60)
                         cgatcTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGG                                                                                                                             <  SRR19973069.3595919/145‑1 (MQ=60)
                         CACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGG                                                                                                                             <  SRR19973069.2537841/150‑1 (MQ=60)
                          ACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGG                                                                                                                            <  SRR19973069.489411/150‑1 (MQ=60)
                          ACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGG                                                                                                                            <  SRR19973069.489418/150‑1 (MQ=60)
                          ACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGG                                                                                                                            <  SRR19973069.489489/150‑1 (MQ=60)
                            AGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTT                                                                                                                          <  SRR19973069.139556/150‑1 (MQ=60)
                            AGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTT                                                                                                                          <  SRR19973069.958559/150‑1 (MQ=60)
                                   CCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATG                                                                                                                   <  SRR19973069.222267/150‑1 (MQ=60)
                                     AGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATAAGCTATCGGAAGTCATACCAATCCTTATGTTTCAGAGGGTTAGCCAAGGC                                                                                                                 >  SRR19973069.35166/1‑150 (MQ=60)
                                          CGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCA                                                                                                            <  SRR19973069.2435679/150‑1 (MQ=60)
                                              CAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAAT                                                                                                        <  SRR19973069.314426/150‑1 (MQ=60)
                                              CAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAAT                                                                                                        <  SRR19973069.2738987/150‑1 (MQ=60)
                                              CAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCGTATGTTTAAGAGGGTTAGCCAAGGCACCCAAAAA                                                                                                        >  SRR19973069.2622670/1‑150 (MQ=60)
                                                      CAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAA                                                                                                <  SRR19973069.1002001/150‑1 (MQ=60)
                                                          CCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCAC                                                                                            <  SRR19973069.980237/150‑1 (MQ=60)
                                                           CGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCAGTCCGTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACG                                                                                           >  SRR19973069.826465/1‑150 (MQ=60)
                                                           CGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATAGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCCTGGCACCCAAAATATCAGAAATCACC                                                                                           >  SRR19973069.826480/1‑150 (MQ=60)
                                                             ACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTT                                                                                         <  SRR19973069.107617/150‑1 (MQ=60)
                                                              CCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCCCCCAAAATATCAGAAATCACGTTA                                                                                        >  SRR19973069.1290179/1‑150 (MQ=60)
                                                               CATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAA                                                                                       >  SRR19973069.2753805/1‑150 (MQ=60)
                                                               CATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCGATCGTAAGTCCAACCATTCCTTATGTTTCAGAGGGTTAGCCCTGGCACCCCAAATATCAGAAAACACGTTAA                                                                                       >  SRR19973069.379264/1‑150 (MQ=60)
                                                               CATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTAGCTTTATAAGCTATCGTAAGTCATAACATTCCTTATTTTTAAGAGGGTTATCCATGGCACCCAAAATATAAGAAATCACGTTAA                                                                                       >  SRR19973069.379266/1‑150 (MQ=60)
                                                                ATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAAC                                                                                      <  SRR19973069.1830022/150‑1 (MQ=60)
                                                                ATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAAC                                                                                      <  SRR19973069.1830092/150‑1 (MQ=60)
                                                                      GAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCTTACGTTTTGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAAT                                                                                <  SRR19973069.3771501/150‑1 (MQ=60)
                                                                      GAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAAT                                                                                <  SRR19973069.3498461/150‑1 (MQ=60)
                                                                       AGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATG                                                                               <  SRR19973069.1286469/150‑1 (MQ=60)
                                                                       AGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATG                                                                               <  SRR19973069.696513/150‑1 (MQ=60)
                                                                       AGGCGTCACTATAAAATTTGCGCCAGGCAGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATG                                                                               <  SRR19973069.696506/150‑1 (MQ=60)
                                                                         GCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGAC                                                                             <  SRR19973069.2289870/150‑1 (MQ=60)
                                                                          CGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACC                                                                            <  SRR19973069.1903527/150‑1 (MQ=60)
                                                                              ACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTG                                                                        >  SRR19973069.2823393/1‑150 (MQ=60)
                                                                              ACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAAAGACCAGTG                                                                        >  SRR19973069.895772/1‑150 (MQ=60)
                                                                              ACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAAAAATGACCAGTG                                                                        >  SRR19973069.3436704/1‑150 (MQ=60)
                                                                                TATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAG                                                                      <  SRR19973069.635439/150‑1 (MQ=60)
                                                                                TATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAG                                                                      <  SRR19973069.447975/150‑1 (MQ=60)
                                                                                TATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACCTTAACAATAATGACCAGTGAG                                                                      >  SRR19973069.2986508/1‑150 (MQ=60)
                                                                                TATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCAATCCTTATGTTTCAGAGGGTTAGCCAAGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAG                                                                      >  SRR19973069.2986589/1‑150 (MQ=60)
                                                                                     AATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACA                                                                 <  SRR19973069.3305036/150‑1 (MQ=60)
                                                                                     AATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACA                                                                 <  SRR19973069.3305136/150‑1 (MQ=60)
                                                                                     AATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACA                                                                 <  SRR19973069.3305161/150‑1 (MQ=60)
                                                                                       TTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACA                                                               >  SRR19973069.1689800/1‑150 (MQ=60)
                                                                                       TTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACA                                                               >  SRR19973069.2498720/1‑150 (MQ=60)
                                                                                            GCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTAGCAGTAAAAGCTATCGTAAGTAATACCAATGCGTATGTTTCAGATGGTTAGCCAATGCACACAAAAAATCAGAAATCACCTTAAAAAAAAAGAAAAGTGACCCACACACCATC                                                          >  SRR19973069.2036931/1‑150 (MQ=32)
                                                                                             CCAGGCGTTACTCAGCGTGCCCGTCGTGATCTTTTAGCTGTATACGCTATCGTAAGTCATACCATTCCTTATTTTTCAGAGGTTTAGCCATGGCACCCATAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCA                                                         <  SRR19973069.1876273/150‑1 (MQ=60)
                                                                                             CCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCA                                                         <  SRR19973069.2908503/150‑1 (MQ=60)
                                                                                             CCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATAAGCTATCGTAAGTCAAACCATTACTTATGTTTAAGAGGGTTATCCATGGCACCCAAAATATCAGAAATAACGTTAAAAATAATTAACAGTGAGCCACACACCAACA                                                         >  SRR19973069.37797/1‑150 (MQ=60)
                                                                                              CAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCGTATGTTTCAGAGGGTTAGCCAAGGCACCCAAAATATCAGAAATCACGTTAACAAAAATGACCAGTGAGCCACACACCATCAC                                                        >  SRR19973069.2535592/1‑150 (MQ=60)
                                                                                                  CGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCG                                                    <  SRR19973069.3440517/150‑1 (MQ=60)
                                                                                                   GGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGGTAACAATAATGACCAGTGAGCCACACACCATCACTCCGG                                                   >  SRR19973069.1832116/1‑150 (MQ=60)
                                                                                                     TACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCG                                                 <  SRR19973069.3491287/150‑1 (MQ=60)
                                                                                                     TACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCG                                                 <  SRR19973069.3491280/150‑1 (MQ=60)
                                                                                                          AGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCATTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACACTAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAAT                                            <  SRR19973069.2915764/150‑1 (MQ=60)
                                                                                                          AGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAAT                                            <  SRR19973069.3020792/150‑1 (MQ=60)
                                                                                                           GCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATG                                           <  SRR19973069.2590057/150‑1 (MQ=60)
                                                                                                            CGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGG                                          <  SRR19973069.388705/150‑1 (MQ=60)
                                                                                                                     CGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAAT                                 <  SRR19973069.3660001/150‑1 (MQ=60)
                                                                                                                     CGGGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAAT                                 <  SRR19973069.3659998/150‑1 (MQ=60)
                                                                                                                       GGCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCC                               <  SRR19973069.2143042/150‑1 (MQ=60)
                                                                                                                        GCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCT                              <  SRR19973069.2243168/150‑1 (MQ=60)
                                                                                                                        GCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCT                              <  SRR19973069.1743856/150‑1 (MQ=60)
                                                                                                                        GCGCTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCT                              <  SRR19973069.2015622/150‑1 (MQ=60)
                                                                                                                           CTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCT                           <  SRR19973069.3474590/150‑1 (MQ=60)
                                                                                                                           CTTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCT                           <  SRR19973069.663323/150‑1 (MQ=60)
                                                                                                                            TTTTCGCTGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGGACTCCTGCTG                          >  SRR19973069.2296708/1‑150 (MQ=60)
                                                                                                                                   TGTATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATG                   <  SRR19973069.2878227/150‑1 (MQ=60)
                                                                                                                                      ATACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCG                >  SRR19973069.2776055/1‑150 (MQ=60)
                                                                                                                                       TACGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGT               >  SRR19973069.3521873/1‑150 (MQ=60)
                                                                                                                                         CGCTATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTA             <  SRR19973069.3171786/150‑1 (MQ=60)
                                                                                                                                            TATCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATT          >  SRR19973069.2350901/1‑150 (MQ=60)
                                                                                                                                              TCGTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAA        <  SRR19973069.3493416/150‑1 (MQ=60)
                                                                                                                                                GTAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACC      >  SRR19973069.1639926/1‑150 (MQ=60)
                                                                                                                                                 TAAGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCA     >  SRR19973069.799010/1‑150 (MQ=60)
                                                                                                                                                   AGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGC   >  SRR19973069.612609/1‑150 (MQ=60)
                                                                                                                                                   AGTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGC   >  SRR19973069.612602/1‑150 (MQ=60)
                                                                                                                                                    GTCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCG  <  SRR19973069.229071/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
AGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCG  >  CP009273/1348623‑1348920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑