Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,351,022 G→T 100% R116S (CGT→AGT)  sapA ← antimicrobial peptide transport ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,351,0220GT99.5% 691.8 / ‑4.3 217R116S (CGT→AGT) sapAantimicrobial peptide transport ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (105/111);  total (105/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 217 total aligned reads displayed.

CAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTC  >  CP009273/1350879‑1351165
                                                                                                                                               |                                                                                                                                               
cAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTg                                                                                                                                             >  2:2325395/1‑148 (MQ=255)
  gTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTggg                                                                                                                                           <  1:2526726/148‑1 (MQ=255)
   tttGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGa                                                                                                                                          >  1:129893/1‑148 (MQ=255)
   tttGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGa                                                                                                                                          >  2:1836904/1‑148 (MQ=255)
    ttGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAg                                                                                                                                         >  1:1844130/1‑148 (MQ=255)
     tGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGt                                                                                                                                        <  2:3245328/148‑1 (MQ=255)
         gACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAAc                                                                                                                                    >  1:2813807/1‑148 (MQ=255)
          actcTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAAATCGCAGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTaaaac                                                                                                                                   >  1:2565727/4‑146 (MQ=255)
           ctcTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCa                                                                                                                                  <  1:3302543/146‑1 (MQ=255)
               tttCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCg                                                                                                                              >  2:1277770/1‑148 (MQ=255)
               tttCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCg                                                                                                                              >  1:182622/1‑148 (MQ=255)
                ttCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCgg                                                                                                                             <  1:2498885/148‑1 (MQ=255)
                  cACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGtt                                                                                                                           <  1:567391/148‑1 (MQ=255)
                   aCGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCGGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGttt                                                                                                                          <  1:1170846/148‑1 (MQ=255)
                     gTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGttttt                                                                                                                        <  1:727121/148‑1 (MQ=255)
                      ttATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGtttttt                                                                                                                       >  1:847605/1‑148 (MQ=255)
                       tATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTg                                                                                                                      >  2:2860583/1‑148 (MQ=255)
                         tCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGaa                                                                                                                    <  2:539578/148‑1 (MQ=255)
                          cGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGaaa                                                                                                                   <  2:2658215/148‑1 (MQ=255)
                           ggCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAAc                                                                                                                  <  2:2191661/148‑1 (MQ=255)
                            gCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACg                                                                                                                 >  2:3412695/1‑148 (MQ=255)
                             cAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAAAgg                                                                                                                >  1:837438/1‑148 (MQ=255)
                               aaTTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGaa                                                                                                              <  1:1796918/148‑1 (MQ=255)
                               aaTTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAAAGGaa                                                                                                              >  1:493121/1‑148 (MQ=255)
                                aTTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAAc                                                                                                             <  2:2484509/148‑1 (MQ=255)
                                aTTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAAc                                                                                                             >  2:563875/1‑148 (MQ=255)
                                 ttGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACa                                                                                                            <  2:1956958/148‑1 (MQ=255)
                                  tGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCTTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAt                                                                                                           <  2:3121183/148‑1 (MQ=255)
                                  tGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAt                                                                                                           >  2:512066/1‑148 (MQ=255)
                                      ggCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCCTTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTTAACGGTACAAcgcc                                                                                                       >  1:3385381/1‑147 (MQ=255)
                                      ggCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTTAAAGGAACaaaccg                                                                                                       >  1:47856/1‑143 (MQ=255)
                                      ggCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATcgcg                                                                                                       <  2:96222/148‑1 (MQ=255)
                                       gCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGTAACGGAACATCGCGa                                                                                                      >  2:2370594/1‑148 (MQ=255)
                                       gCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGa                                                                                                      >  2:168080/1‑148 (MQ=255)
                                           tCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCa                                                                                                  >  2:2758458/1‑148 (MQ=255)
                                           tCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCa                                                                                                  >  2:160/1‑148 (MQ=255)
                                            cGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAg                                                                                                 <  1:1981621/148‑1 (MQ=255)
                                                 tAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAACGGAACATCGCGACGCAGGTGGa                                                                                            >  1:1461613/1‑148 (MQ=255)
                                                 tAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGa                                                                                            <  2:1135450/148‑1 (MQ=255)
                                                     ggAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCg                                                                                        <  1:793772/148‑1 (MQ=255)
                                                     ggAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCg                                                                                        <  1:2555998/148‑1 (MQ=255)
                                                       aaGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGAt                                                                                      <  2:747389/148‑1 (MQ=255)
                                                                 cGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGcc                                                                            >  2:2963108/1‑148 (MQ=255)
                                                                  gTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCg                                                                           <  1:1374528/148‑1 (MQ=255)
                                                                     gACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTg                                                                        <  2:1828723/148‑1 (MQ=255)
                                                                     gACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTg                                                                        <  1:1918170/148‑1 (MQ=255)
                                                                      aCGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGTAGCGATAGGTCGCGCCGTTGt                                                                       >  1:1034086/1‑148 (MQ=255)
                                                                       cGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTc                                                                      <  2:2772186/148‑1 (MQ=255)
                                                                          tATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTTAACGGAACCTCGCGACGCAGGTGGAAGCGAGAGGTGGCGCCGGTGTCGAg                                                                   >  1:2734843/1‑148 (MQ=255)
                                                                          tATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAg                                                                   >  2:2893924/1‑148 (MQ=255)
                                                                          tATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAg                                                                   >  1:1169574/1‑148 (MQ=255)
                                                                          tATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAg                                                                   <  2:1785712/148‑1 (MQ=255)
                                                                            tGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTa                                                                 >  2:922331/1‑148 (MQ=255)
                                                                                aCGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTc                                                             <  2:3483431/148‑1 (MQ=255)
                                                                                aCGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTc                                                             <  1:1886021/148‑1 (MQ=255)
                                                                                 cGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTcc                                                            <  2:2482525/148‑1 (MQ=255)
                                                                                 cGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTcc                                                            <  1:1627421/148‑1 (MQ=255)
                                                                                     ttgttgCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGc                                                        <  1:2802956/148‑1 (MQ=255)
                                                                                       gttgCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCtt                                                      <  1:3100256/148‑1 (MQ=255)
                                                                                              tCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCaa                                               >  2:3300844/1‑148 (MQ=255)
                                                                                                  aaaTTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTc                                           <  1:2325395/148‑1 (MQ=255)
                                                                                                   aaTTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTcc                                          <  2:351706/148‑1 (MQ=255)
                                                                                                   aaTTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTcc                                          >  2:2094932/1‑148 (MQ=255)
                                                                                                     ttCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAAAGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTGGAGTACGTCCCCGCTTTAGGCACCTTCCgg                                        >  1:2419512/1‑148 (MQ=255)
                                                                                                     ttCGCAGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCgg                                        <  2:3219563/148‑1 (MQ=255)
                                                                                                      tCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCACGTTCCGGc                                       >  2:3090011/1‑148 (MQ=255)
                                                                                                      tCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGc                                       >  2:1343297/1‑148 (MQ=255)
                                                                                                          tGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCa                                   <  2:152401/148‑1 (MQ=255)
                                                                                                            gAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAgg                                 <  1:922331/148‑1 (MQ=255)
                                                                                                            gAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAgg                                 >  1:1121418/1‑148 (MQ=255)
                                                                                                                 tAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATa                            <  2:601486/148‑1 (MQ=255)
                                                                                                                   aaCACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAgg                          >  2:2432026/1‑148 (MQ=255)
                                                                                                                    acacCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGt                         <  2:2565579/148‑1 (MQ=255)
                                                                                                                    acacCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGt                         <  1:2818699/148‑1 (MQ=255)
                                                                                                                    acacCACATCGTAGTCATTCATTTTACTTTTGGGATTAAACCATTCTGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCTAGTACTTCCCATCTTTCGGCAAGTTCCGGCATCAGGCGATAGGt                         <  1:43849/148‑1 (MQ=255)
                                                                                                                     caccacATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGta                        <  2:2813807/148‑1 (MQ=255)
                                                                                                                      accacATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGCTAGGtat                       >  1:1095694/1‑148 (MQ=255)
                                                                                                                      accacATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCCGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGtat                       >  1:1095617/1‑148 (MQ=255)
                                                                                                                       ccacATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGtata                      <  1:2902215/148‑1 (MQ=255)
                                                                                                                       ccacATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTAGGCAAGTTCCGGCATCAGGCGATAGGtata                      >  1:2788263/1‑148 (MQ=255)
                                                                                                                         acaTCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAAGGAACAACGCGACCCAGGTGGTAGCGATATGTCGCGCCGGTGTGGAGTACTTACCACCGTTCGGCAACTTACGGCAAAAGGCGCTAGGTATAgg                    >  1:624223/1‑148 (MQ=255)
                                                                                                                         acaTCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATTGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAgg                    <  1:2860583/148‑1 (MQ=255)
                                                                                                                            tcgtcgGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGAt                 <  2:129893/148‑1 (MQ=255)
                                                                                                                            tcgtcgGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGAt                 >  2:2875646/1‑148 (MQ=255)
                                                                                                                             cgtcgGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGCTAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATc                >  2:3037860/1‑148 (MQ=255)
                                                                                                                              gtcgGCATTCATTTTACTAGGGGCAGTAAACCAGTCTGTTTTTTGAAAGGGAACTTCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTAGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCTATAGGTATAGGGATCg               <  2:1946816/148‑1 (MQ=255)
                                                                                                                                 ggCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACa            <  2:3258411/148‑1 (MQ=255)
                                                                                                                                  gCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACAt           >  2:382294/1‑148 (MQ=255)
                                                                                                                                   cattcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATc          <  1:887014/148‑1 (MQ=255)
                                                                                                                                   cattcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATc          <  2:2114283/148‑1 (MQ=255)
                                                                                                                                   cattcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATc          >  2:809420/1‑148 (MQ=255)
                                                                                                                                     ttcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCca        >  2:56785/1‑148 (MQ=255)
                                                                                                                                      tcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAACCGAACATCGCGACCCACGTGGAACCGCTAGGTCGCGCCCTTGGCGAGTACTTACCACCTTTAGGCAACTTCCGGCAACAGGCGATAGGTATAGGGATAGACAACcag       >  1:704324/1‑148 (MQ=255)
                                                                                                                                      tcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAAGGAACATAGCCACCCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCcag       >  1:204232/1‑148 (MQ=255)
                                                                                                                                      tcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAAGGAACAAAGCGACGCAGGTGGAGGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCACGTTCCGGCCTCAGGCGATAGGTATAGGGATCGACATCcag       >  1:3065386/1‑148 (MQ=255)
                                                                                                                                      tcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCcag       <  2:182622/148‑1 (MQ=255)
                                                                                                                                      tcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATAcag       >  2:515304/1‑148 (MQ=255)
                                                                                                                                      tcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACCGAACAACGCGACCCAGGTGGAAGCGATAGGTCGCGCCGGTGTCGAGTACTTCCCCGCTTTCGGCCAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCcag       >  1:3406216/1‑148 (MQ=255)
                                                                                                                                       cattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCcagc      >  1:2253807/1‑148 (MQ=255)
                                                                                                                                           ttACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTc  >  2:2728993/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                               
CAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTC  >  CP009273/1350879‑1351165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCAT  >  CP009273/1350873‑1351171
                                                                                                                                                     |                                                                                                                                                     
ATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACT                                                                                                                                                       >  SRR19973065.3017260/1‑150 (MQ=60)
ATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACT                                                                                                                                                       >  SRR19973065.3017341/1‑150 (MQ=60)
ATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACT                                                                                                                                                       <  SRR19973065.1547952/150‑1 (MQ=60)
   ATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGT                                                                                                                                                    <  SRR19973065.2830078/150‑1 (MQ=60)
      CAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGG                                                                                                                                                 >  SRR19973065.2673033/1‑150 (MQ=60)
      CAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGG                                                                                                                                                 >  SRR19973065.1840791/1‑150 (MQ=60)
       AGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGA                                                                                                                                                >  SRR19973065.131008/1‑150 (MQ=60)
        GTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAG                                                                                                                                               <  SRR19973065.2312077/150‑1 (MQ=60)
        GTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAG                                                                                                                                               <  SRR19973065.2542550/150‑1 (MQ=60)
        GTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAG                                                                                                                                               <  SRR19973065.2542589/150‑1 (MQ=60)
        GTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAG                                                                                                                                               >  SRR19973065.1856290/1‑150 (MQ=60)
        GTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAG                                                                                                                                               >  SRR19973065.164544/1‑150 (MQ=60)
        GTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAG                                                                                                                                               >  SRR19973065.1453047/1‑150 (MQ=60)
        GTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAG                                                                                                                                               >  SRR19973065.1211195/1‑150 (MQ=60)
             CGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAAC                                                                                                                                          >  SRR19973065.2831199/1‑150 (MQ=60)
             CGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAAC                                                                                                                                          <  SRR19973065.860049/150‑1 (MQ=60)
              GGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACC                                                                                                                                         >  SRR19973065.2581606/1‑150 (MQ=60)
              GGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAAATCGCAGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAAAC                                                                                                                                         >  SRR19973065.2581754/1‑150 (MQ=60)
               GACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCA                                                                                                                                        >  SRR19973065.3264627/1‑150 (MQ=60)
                ACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAG                                                                                                                                       >  SRR19973065.1146806/1‑150 (MQ=60)
                 CTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGT                                                                                                                                      <  SRR19973065.3322655/150‑1 (MQ=60)
                  TCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTC                                                                                                                                     <  SRR19973065.3409444/150‑1 (MQ=60)
                  TCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTC                                                                                                                                     <  SRR19973065.1828389/150‑1 (MQ=60)
                   CTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCG                                                                                                                                    >  SRR19973065.184066/1‑150 (MQ=60)
                      TTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTT                                                                                                                                 <  SRR19973065.2514601/150‑1 (MQ=60)
                        CACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTT                                                                                                                               <  SRR19973065.570990/150‑1 (MQ=60)
                         ACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCCTTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTT                                                                                                                              >  SRR19973065.1577790/1‑150 (MQ=60)
                         ACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCGGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTT                                                                                                                              <  SRR19973065.1178307/150‑1 (MQ=60)
                         ACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCGGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTT                                                                                                                              <  SRR19973065.1178094/150‑1 (MQ=60)
                          CGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTT                                                                                                                             >  SRR19973065.853099/1‑150 (MQ=60)
                           GTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTG                                                                                                                            <  SRR19973065.731995/150‑1 (MQ=60)
                              ATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAA                                                                                                                         <  SRR19973065.118832/150‑1 (MQ=60)
                              ATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAA                                                                                                                         <  SRR19973065.2809093/150‑1 (MQ=60)
                                 GGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGG                                                                                                                      >  SRR19973065.842895/1‑150 (MQ=60)
                                 GGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAAAGG                                                                                                                      >  SRR19973065.842892/1‑150 (MQ=60)
                                   CAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAAAGGAA                                                                                                                    >  SRR19973065.496442/1‑150 (MQ=60)
                                     AATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACA                                                                                                                  <  SRR19973065.1808721/150‑1 (MQ=60)
                                        TGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCG                                                                                                               <  SRR19973065.615646/150‑1 (MQ=60)
                                          CAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCCTTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTttaacggtacaacgcc                                                                                                             >  SRR19973065.3406084/1‑134 (MQ=60)
                                          CAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTttaaaggaacaaagcg                                                                                                             >  SRR19973065.48806/1‑134 (MQ=60)
                                          CAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTttaaaggaacaaaccg                                                                                                             >  SRR19973065.48404/1‑134 (MQ=60)
                                          CAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAAaaaagcg                                                                                                             >  SRR19973065.189865/1‑143 (MQ=60)
                                          CAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCAGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTTAACGGAACATAGCG                                                                                                             >  SRR19973065.2784186/1‑150 (MQ=60)
                                          CAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGAAGTTATGCCACGGGTTTTTGCGGTAAAAAAATCGCTTGAAGGTAAACACCACATCGTCGGCATTCATTTTAATATTGGGAGTAAACCAGTCGGTTTTTTttaaaagaaaaaaccc                                                                                                             >  SRR19973065.1021491/1‑134 (MQ=60)
                                                GTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGTAAAGGAAAATCGCGACccac                                                                                                       >  SRR19973065.1005336/1‑146 (MQ=60)
                                                  CGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGT                                                                                                     <  SRR19973065.1994504/150‑1 (MQ=60)
                                                  CGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGT                                                                                                     <  SRR19973065.1994486/150‑1 (MQ=60)
                                                     AGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAACGGAACATCGCGACGCAGGTGGA                                                                                                  >  SRR19973065.1471006/1‑150 (MQ=60)
                                                       TAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAACCGAACATCCCGACGCACGTGGAAG                                                                                                >  SRR19973065.2474917/1‑150 (MQ=60)
                                                           GGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGAT                                                                                            <  SRR19973065.799024/150‑1 (MQ=60)
                                                           GGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGAT                                                                                            <  SRR19973065.2571985/150‑1 (MQ=60)
                                                                        GTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTT                                                                               <  SRR19973065.1383598/150‑1 (MQ=60)
                                                                         TTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTG                                                                              <  SRR19973065.892779/150‑1 (MQ=60)
                                                                          TGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGTAGCGATAGGTCGCGCCGTTGT                                                                             >  SRR19973065.1040931/1‑150 (MQ=60)
                                                                           GACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTC                                                                            <  SRR19973065.1930752/150‑1 (MQ=60)
                                                                              GTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTTAACGGAACCTCGCGACGCAGGTGGAAGCGAGAGGTGGCGCCGGTGTCGAG                                                                         >  SRR19973065.2751853/1‑150 (MQ=60)
                                                                              GTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAG                                                                         >  SRR19973065.1177029/1‑150 (MQ=60)
                                                                              GTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAAAAGAACATAGCGACGCAGGTGGTAGCGAaatgtagccccggtgtagag                                                                         >  SRR19973065.771621/1‑130 (MQ=60)
                                                                                 ATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTAC                                                                      <  SRR19973065.722840/150‑1 (MQ=60)
                                                                                 ATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTAC                                                                      <  SRR19973065.722732/150‑1 (MQ=60)
                                                                                     CACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGACATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGGTGTCGAGTACTTCC                                                                  >  SRR19973065.3080086/1‑150 (MQ=60)
                                                                                      ACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCC                                                                 <  SRR19973065.1898432/150‑1 (MQ=60)
                                                                                      ACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCC                                                                 >  SRR19973065.2272380/1‑150 (MQ=60)
                                                                                       CGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCA                                                                <  SRR19973065.1638328/150‑1 (MQ=60)
                                                                                           TTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTT                                                            <  SRR19973065.2820310/150‑1 (MQ=60)
                                                                                           TTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTT                                                            <  SRR19973065.2820515/150‑1 (MQ=60)
                                                                                             GTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTC                                                          <  SRR19973065.3119182/150‑1 (MQ=60)
                                                                                                  GGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAA                                                     <  SRR19973065.1322202/150‑1 (MQ=60)
                                                                                                      AAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTC                                                 <  SRR19973065.2379465/150‑1 (MQ=60)
                                                                                                        AAATTCGCTTGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCG                                               <  SRR19973065.2339952/150‑1 (MQ=60)
                                                                                                        AAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAACGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTTTGGAGTAATTACCAGCGTTCGGCAAGTTACG                                               >  SRR19973065.632625/1‑150 (MQ=60)
                                                                                                         AATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAACGCGACGCAGGTGGGAGCGCTAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCccggtccgg                                              >  SRR19973065.2733418/1‑141 (MQ=60)
                                                                                                         AATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAAAGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTGGAGTACGTCCCCGCTTTAGGCACCTTCCGG                                              >  SRR19973065.2434766/1‑150 (MQ=60)
                                                                                                             CGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATC                                          >  SRR19973065.2850714/1‑150 (MQ=60)
                                                                                                             CGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAAAGGAACAAAGCCACGCAGGTGGTAGCGATAGGTAGCGCCGTTGTCGAGTAATTCACAGCTTTagggcaggtgccgcata                                          >  SRR19973065.163999/1‑133 (MQ=60)
                                                                                                                TGGAATGTAAACACCACATCTTCGGAATTCATATTACTATTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGG                                       <  SRR19973065.2081040/150‑1 (MQ=60)
                                                                                                                TGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGG                                       >  SRR19973065.1128727/1‑150 (MQ=60)
                                                                                                                  GAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCG                                     <  SRR19973065.928375/150‑1 (MQ=60)
                                                                                                                    AGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGAT                                   <  SRR19973065.570142/150‑1 (MQ=60)
                                                                                                                          ACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGCTAGGTAT                             >  SRR19973065.1102904/1‑150 (MQ=60)
                                                                                                                          ACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCCGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTAT                             >  SRR19973065.1102827/1‑150 (MQ=60)
                                                                                                                          ACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTAT                             <  SRR19973065.2836113/150‑1 (MQ=60)
                                                                                                                          ACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTAT                             <  SRR19973065.2836007/150‑1 (MQ=60)
                                                                                                                          ACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGGTGTGGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTAT                             >  SRR19973065.1102907/1‑150 (MQ=60)
                                                                                                                          ACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATAGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCCGCTTTCGGCCAGTTCCGGCATCAGGCGATAGGTAT                             >  SRR19973065.1102900/1‑150 (MQ=60)
                                                                                                                          ACACCACATCGTAGTCATTCATTTTACTTTTGGGATTAAACCATTCTGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCTAGTACTTCCCATCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTAT                             <  SRR19973065.44371/150‑1 (MQ=60)
                                                                                                                           CACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTAGGCAAGTTCCGGCATCAGGCGATAGGTATA                            >  SRR19973065.2805549/1‑150 (MQ=60)
                                                                                                                             CCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTTAACCGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGGTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCCTCAGGCGATAGGTATAGG                          >  SRR19973065.1096432/1‑150 (MQ=60)
                                                                                                                             CCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAAGGAACAACGCGACCCAGGTGGTAGCGATATGTCGCGCCGGTGTGGAGTACTTACCACCGTTCGGCAACTTACGGCAAAAGGCGCTAGGTATAGG                          >  SRR19973065.628263/1‑150 (MQ=60)
                                                                                                                             CCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGG                          <  SRR19973065.2919821/150‑1 (MQ=60)
                                                                                                                             CCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGG                          <  SRR19973065.2919916/150‑1 (MQ=60)
                                                                                                                              CACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGG                         <  SRR19973065.44429/150‑1 (MQ=60)
                                                                                                                              CACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGG                         <  SRR19973065.44176/150‑1 (MQ=60)
                                                                                                                               ACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATTGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGA                        <  SRR19973065.2878148/150‑1 (MQ=60)
                                                                                                                                 ATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAAGGAACATAGCGACCCAGGTGGAAGCGATAGGTCGCGCCGGTTTGGAGTACTTACCAGCTTTAGGCAAATTCCGGCATCAGGCGAaatgtatatggata                      >  SRR19973065.2552908/1‑136 (MQ=60)
                                                                                                                                 ATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAAGGAAAATAGCGACCCAGGTGGAAGCCATAGGTAGCGCCGATGGCGAGTAATTAACAGCATTCGGCAACTTCCGGCCTAAGGCCATAGGTATATGGATC                      >  SRR19973065.2901662/1‑150 (MQ=45)
                                                                                                                                  TCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCG                     <  SRR19973065.822037/150‑1 (MQ=60)
                                                                                                                                      CGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACAT                 <  SRR19973065.970406/150‑1 (MQ=60)
                                                                                                                                         CATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCA              <  SRR19973065.1920513/150‑1 (MQ=60)
                                                                                                                                         CATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCA              <  SRR19973065.892647/150‑1 (MQ=60)
                                                                                                                                          ATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAACCGAACATCGCGACCCACGTGGAACCGCTAGGTCGCGCCCTTGGCGAGTACTTACCACCTTTAGGCAACTTCCGGCAACAGGCGATAGGTATAGGGATAGACAACCAG             >  SRR19973065.709008/1‑150 (MQ=60)
                                                                                                                                          ATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAAGGAACATCGCCACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTAATTACCAGCTTTCGGCAATTTCCGGCATCAGGCGATAGGTATAGGGATCGACAaccac             >  SRR19973065.3364154/1‑145 (MQ=60)
                                                                                                                                          ATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAAGGAACATAGCCACCCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAG             >  SRR19973065.205795/1‑150 (MQ=60)
                                                                                                                                          ATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAAGGAACAAAGCGACGCAGGTGGAGGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCACGTTCCGGCCTCAGGCGATAGGTATAGGGATCGACATCCAG             >  SRR19973065.3084128/1‑150 (MQ=60)
                                                                                                                                          ATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAAGGAAAAACGCGACGCAGGTGGTAGCGATATGTCGCGCCGTTTTGGAGTAATTACCACCTTTAGGCCAGTTCCGGCATCAGGCGCTAGGTATAGGGATCGACATACAG             >  SRR19973065.3084251/1‑150 (MQ=60)
                                                                                                                                          ATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAACGAACATAGCGACGCAGGTGGAACCGCTAGGTCGCGCCGTTTTAGAGTAATTACCAGCTTTAGGCAACTTACGGCATCAAGCGATAGGTATAGGGATAGACATCCCG             >  SRR19973065.205766/1‑150 (MQ=60)
                                                                                                                                          ATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACCGAACAACGCGACCCAGGTGGAAGCGATAGGTCGCGCCGGTGTCGAGTACTTCCCCGCTTTCGGCCAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAG             >  SRR19973065.3427027/1‑150 (MQ=60)
                                                                                                                                           TTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGC            >  SRR19973065.2268086/1‑150 (MQ=60)
                                                                                                                                              ATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGT         <  SRR19973065.1849019/150‑1 (MQ=60)
                                                                                                                                               TTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTC        <  SRR19973065.3510321/150‑1 (MQ=60)
                                                                                                                                                     TAGTGGGAGTAAACCAGTCGGTTTTTTTAAACGGAACATCGCGACGCAGGTGGGAGCGATAGGTCGCGCCGTTGTGGAGTACTTCCCAGCTTTCGGCCAGTTCCGGCCTCAGGCGATAGGGATAGGGATCGACAACCAGCACTCGATCAT  >  SRR19973065.2131787/1‑150 (MQ=60)
                                                                                                                                                     TAGTGGGAGTAAACCAGTCGGTTTTTTTAAAAGGAACAAAGCGCCCCACGTGGAAGCGAAATGTCGCGCCGTTTTCGAGTACTTACCCGCATTCGGCAAATTGCGGCAACAGGCGCGAGGTATAGGGATCGACAaacaccactcgatcct  >  SRR19973065.655266/1‑134 (MQ=30)
                                                                                                                                                     TAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCAT  <  SRR19973065.960602/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
ATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCAT  >  CP009273/1350873‑1351171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑