Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F394 I0 R1	1342	215.0	7134094	96.2%	6862998	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,351,022	G→T	100%	R116S (CGT→AGT)	sapA ←	antimicrobial peptide transport ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,351,022	0	G	T	97.1%	625.4 / 9.1	210	R116S (CGT→AGT)	sapA	antimicrobial peptide transport ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base G (2/4); new base T (108/96); total (110/100)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 210 total aligned reads displayed.

CCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGT  >  CP009273/1350878‑1351164

ccAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGt                                                                                                                                             >  2:3343708/1‑148 (MQ=255)

  aGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTgg                                                                                                                                           >  2:1481565/1‑148 (MQ=255)

  aGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTgg                                                                                                                                           >  1:3042844/1‑148 (MQ=255)

   gTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTggg                                                                                                                                          <  1:69810/148‑1 (MQ=255)

   gTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATAGTCGGCATTCATTTTACTAGTggg                                                                                                                                          >  2:2858449/1‑148 (MQ=255)

    tttGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGa                                                                                                                                         <  2:441621/148‑1 (MQ=255)

      tGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGt                                                                                                                                       <  1:3233430/148‑1 (MQ=255)

          gACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAAc                                                                                                                                   >  2:811184/1‑148 (MQ=255)

          gACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAAc                                                                                                                                   <  2:767295/148‑1 (MQ=255)

          gACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAAc                                                                                                                                   >  2:828028/1‑148 (MQ=255)

             tcTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAg                                                                                                                                >  2:1441587/2‑148 (MQ=255)

             gCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAg                                                                                                                                <  1:1832953/148‑1 (MQ=255)

                tttCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCg                                                                                                                             <  2:131856/148‑1 (MQ=255)

                tttCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCg                                                                                                                             <  2:2678451/148‑1 (MQ=255)

                tttCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCg                                                                                                                             >  2:1024031/1‑148 (MQ=255)

                  tCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGt                                                                                                                           <  1:3341843/148‑1 (MQ=255)

                  tCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGt                                                                                                                           >  1:2543361/1‑148 (MQ=255)

                  tCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGt                                                                                                                           >  1:2543253/1‑148 (MQ=255)

                    aCGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGttt                                                                                                                         >  2:1602121/1‑148 (MQ=255)

                    aCGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGttt                                                                                                                         >  2:1601543/1‑148 (MQ=255)

                       ttATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGtttttt                                                                                                                      >  2:3192008/1‑148 (MQ=255)

                          tCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTgta                                                                                                                   >  1:1238793/1‑146 (MQ=255)

                           cGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGaaa                                                                                                                  <  1:3366138/148‑1 (MQ=255)

                             gCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACg                                                                                                                >  2:3358809/1‑148 (MQ=255)

                               aaaTTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGa                                                                                                              <  1:2102324/148‑1 (MQ=255)

                                 aTTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAAc                                                                                                            <  2:1999583/148‑1 (MQ=255)

                                 aTTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAAAGGTAc                                                                                                            >  1:517081/1‑148 (MQ=255)

                                  ttGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGTACa                                                                                                           >  1:2249251/1‑148 (MQ=255)

                                  ttGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACa                                                                                                           <  2:2397346/148‑1 (MQ=255)

                                  ttGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACa                                                                                                           <  2:663870/148‑1 (MQ=255)

                                  ttGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACa                                                                                                           <  2:607210/148‑1 (MQ=255)

                                  ttGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACa                                                                                                           >  1:2934826/1‑148 (MQ=255)

                                   tGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAt                                                                                                          <  2:2538470/148‑1 (MQ=255)

                                    gCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATc                                                                                                         <  2:3202008/148‑1 (MQ=255)

                                     cAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATcg                                                                                                        <  2:1239981/148‑1 (MQ=255)

                                       ggCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATcgcg                                                                                                      >  1:1775604/1‑148 (MQ=255)

                                              gAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAgg                                                                                               <  1:1598554/148‑1 (MQ=255)

                                                aGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTg                                                                                             <  1:1313917/148‑1 (MQ=255)

                                                  tAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGa                                                                                           <  1:893028/148‑1 (MQ=255)

                                                   aGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGaa                                                                                          <  1:2730793/148‑1 (MQ=255)

                                                     gggAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGc                                                                                        >  2:1841834/1‑148 (MQ=255)

                                                     gggAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAAAGGAACATAGCGACGCAGGTGGTAGc                                                                                        >  1:2598391/1‑148 (MQ=255)

                                                      ggAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCg                                                                                       >  2:1855279/1‑148 (MQ=255)

                                                      ggAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTAGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTTAAACGAAAAAAGCGACGCAGGTGGAAGCg                                                                                       >  1:1570584/1‑148 (MQ=255)

                                                        aaGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGAt                                                                                     >  2:176776/1‑148 (MQ=255)

                                                          gTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGTAACGGAACATCGCGACGCAGGTGGTAGCGATAg                                                                                   >  2:2721430/1‑148 (MQ=255)

                                                                 ccGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCgcgc                                                                            >  1:3183240/1‑148 (MQ=255)

                                                                  cGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGcc                                                                           >  1:2676170/1‑148 (MQ=255)

                                                                    ttGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGt                                                                         <  2:814910/148‑1 (MQ=255)

                                                                    ttGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGt                                                                         <  2:1614904/148‑1 (MQ=255)

                                                                    ttGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGt                                                                         <  2:3383501/148‑1 (MQ=255)

                                                                    ttGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGt                                                                         <  1:1167425/148‑1 (MQ=255)

                                                                    ttGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGt                                                                         <  2:2601189/148‑1 (MQ=255)

                                                                         gTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCg                                                                    <  1:391600/148‑1 (MQ=255)

                                                                           tATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAg                                                                  >  2:2751043/1‑148 (MQ=255)

                                                                                 aCGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTc                                                            >  2:1211549/1‑148 (MQ=255)

                                                                                    ggTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCa                                                         >  2:2547052/1‑148 (MQ=255)

                                                                                    ggTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAACGCGACCCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCa                                                         >  1:437722/1‑148 (MQ=255)

                                                                                       tgttgCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCt                                                      <  1:1602071/148‑1 (MQ=255)

                                                                                       tgttgCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCt                                                      <  2:3373369/148‑1 (MQ=255)

                                                                                        gttgCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCtt                                                     <  1:2790234/148‑1 (MQ=255)

                                                                                              gTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCa                                               <  2:946995/148‑1 (MQ=255)

                                                                                              gTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCa                                               <  2:2469505/148‑1 (MQ=255)

                                                                                               tCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCaa                                              >  1:2355046/1‑148 (MQ=255)

                                                                                                cAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAg                                             >  2:1056940/1‑148 (MQ=255)

                                                                                                cAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAg                                             <  1:1481565/148‑1 (MQ=255)

                                                                                                cAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAg                                             <  2:1115595/148‑1 (MQ=255)

                                                                                                   aaaTTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTc                                          >  1:877926/1‑148 (MQ=255)

                                                                                                    aaTTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTcc                                         <  1:2370903/148‑1 (MQ=255)

                                                                                                    aaTTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTcc                                         <  1:3525797/148‑1 (MQ=255)

                                                                                                      ttCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCgg                                       <  2:1428836/148‑1 (MQ=255)

                                                                                                       tCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGc                                      >  2:720675/1‑148 (MQ=255)

                                                                                                       tCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAACGCGACGCCGGTGGAAGCGCTAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGc                                      >  2:720770/1‑148 (MQ=255)

                                                                                                         gCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCAt                                    <  2:3108679/148‑1 (MQ=255)

                                                                                                             gAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAgg                                <  2:3273932/148‑1 (MQ=255)

                                                                                                             gAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAgg                                >  1:1741446/1‑148 (MQ=255)

                                                                                                                 gTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGAt                            >  2:2321607/1‑148 (MQ=255)

                                                                                                                   aaaCACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGCCGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAg                          >  2:1240228/1‑148 (MQ=255)

                                                                                                                    aaCACCACATAGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAgg                         <  1:2858449/148‑1 (MQ=255)

                                                                                                                      caccacATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGta                       <  2:2267122/148‑1 (MQ=255)

                                                                                                                      caccacATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGta                       >  2:2494868/1‑148 (MQ=255)

                                                                                                                         cacaTCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAACGAACAAAGCGACCCAGGTGGAAGCGCTAGGTCGCGCCGTTTTCGAGTAATTACCAGCATTCGGCAAGTTCCCGCATCACGCGATAGGTATAg                    >  1:2133652/1‑148 (MQ=255)

                                                                                                                         cacaTCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAg                    <  2:966832/148‑1 (MQ=255)

                                                                                                                         cacaTCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAACGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTAGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGCTAGGTATAg                    >  1:1692910/1‑148 (MQ=255)

                                                                                                                          acaTCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAgg                   <  1:1024031/148‑1 (MQ=255)

                                                                                                                           caTCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAggg                  <  1:3481361/148‑1 (MQ=255)

                                                                                                                           caTCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAggg                  <  1:1717696/148‑1 (MQ=255)

                                                                                                                             tcgtcgGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGAt                >  2:494197/1‑148 (MQ=255)

                                                                                                                               gtcgGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCg              <  1:1441587/148‑1 (MQ=255)

                                                                                                                                 cgGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGAc            <  1:2067993/148‑1 (MQ=255)

                                                                                                                                   gCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGCTAGGTATAGGGATCGACAt          >  2:2753939/1‑148 (MQ=255)

                                                                                                                                    cattcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATc         >  2:2431026/1‑148 (MQ=255)

                                                                                                                                     attcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAAAGCGACGCAGGTGGAATCGATAGGTCGCGCCGGTGTCGAGTAATTACCAGCATTAGGCAAGTTACGGCATCAGGCGATAGGTATATGGATCGAAATcc        >  1:2554179/1‑148 (MQ=255)

                                                                                                                                      ttcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGTAACGGAACAAAGCGACGCAGGTGGAAGCGATAGGTCGCGCCGGTGTCGAGTACATACCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATATGGATCGACATCca       >  1:2732702/1‑148 (MQ=255)

                                                                                                                                      ttcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCca       >  2:2902165/1‑148 (MQ=255)

                                                                                                                                       tcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAAGGAACAACGCGACGCAGGTGGAATCGAAAGGTCGCGCCGTTTTCGAGTAAATCCCACCTTTAGGCAACTTACGGCAACACGCCATAGGTATAGGGATAGACAACcac      >  1:730576/1‑147 (MQ=255)

                                                                                                                                       tcattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCcag      >  2:1592851/1‑148 (MQ=255)

                                                                                                                                        cattTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCcagc     <  1:811184/148‑1 (MQ=255)

                                                                                                                                          ttttACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTCTAGGGATCGACATCcagcag   >  2:2715719/1‑148 (MQ=255)

                                                                                                                                          ttttACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCcagcag   >  2:1078861/1‑148 (MQ=255)

CCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGT  >  CP009273/1350878‑1351164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCA  >  CP009273/1350876‑1351170

ATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGT                                                                                                                                                   <  SRR19973066.1191171/150‑1 (MQ=60)

  CCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGG                                                                                                                                                 >  SRR19973066.3063179/1‑150 (MQ=60)

   CAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGG                                                                                                                                                >  SRR19973066.3049196/1‑150 (MQ=60)

     GTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAG                                                                                                                                              <  SRR19973066.70768/150‑1 (MQ=60)

     GTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAG                                                                                                                                              <  SRR19973066.70655/150‑1 (MQ=60)

     GTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAG                                                                                                                                              <  SRR19973066.3275500/150‑1 (MQ=60)

        TGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAA                                                                                                                                           <  SRR19973066.3254784/150‑1 (MQ=60)

        TGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAA                                                                                                                                           <  SRR19973066.2863253/150‑1 (MQ=60)

               GCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTC                                                                                                                                    <  SRR19973066.1846043/150‑1 (MQ=60)

                  TTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGT                                                                                                                                 >  SRR19973066.2560531/1‑150 (MQ=60)

                  TTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGT                                                                                                                                 >  SRR19973066.2560614/1‑150 (MQ=60)

                  TTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGT                                                                                                                                 >  SRR19973066.2560640/1‑150 (MQ=60)

                    TCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTT                                                                                                                               <  SRR19973066.634910/150‑1 (MQ=60)

                    TCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTT                                                                                                                               <  SRR19973066.3364090/150‑1 (MQ=60)

                    GCACGTTATCGGCATATGGCAGGCTGTCGAAGTAGGGGAAGGTGCTGCCGTTGACGTTTTGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTT                                                                                                                               <  SRR19973066.3364096/150‑1 (MQ=60)

                       CGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTT                                                                                                                            >  SRR19973066.895991/1‑150 (MQ=60)

                       CGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTT                                                                                                                            <  SRR19973066.3090867/150‑1 (MQ=60)

                          TATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTtta                                                                                                                         >  SRR19973066.1248835/1‑147 (MQ=60)

                          TATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTtta                                                                                                                         >  SRR19973066.1247629/1‑147 (MQ=60)

                           ATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTttaa                                                                                                                        >  SRR19973066.1591992/1‑146 (MQ=60)

                           ATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCCGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAA                                                                                                                        >  SRR19973066.1935567/1‑150 (MQ=60)

                             CGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACG                                                                                                                      <  SRR19973066.3388581/150‑1 (MQ=60)

                                 AAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAAC                                                                                                                  <  SRR19973066.2116802/150‑1 (MQ=60)

                                 AAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAAAGGTAC                                                                                                                  >  SRR19973066.520890/1‑150 (MQ=60)

                                  AATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAAGGAACA                                                                                                                 >  SRR19973066.457405/1‑150 (MQ=60)

                                  AATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGTACA                                                                                                                 >  SRR19973066.2264757/1‑150 (MQ=60)

                                  AATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACA                                                                                                                 >  SRR19973066.2954348/1‑150 (MQ=60)

                                  AATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACA                                                                                                                 >  SRR19973066.296557/1‑150 (MQ=60)

                                    TTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCCCGGGTTGTTTCGGTCAAAAAATAGCTGGAAGGTAAACACAACATCCTCCTCATTCATTTTACTAGTGTGAGTAAACaacacgggtttttgtaaacgaaactc                                                                                                               >  SRR19973066.2132361/1‑124 (MQ=60)

                                    TTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAAATCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGtaaaggaaaaaa                                                                                                               >  SRR19973066.2132912/1‑138 (MQ=60)

                                       CAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCG                                                                                                            >  SRR19973066.1788305/1‑150 (MQ=60)

                                         GGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGAC                                                                                                          <  SRR19973066.149223/150‑1 (MQ=60)

                                              TCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGG                                                                                                     <  SRR19973066.2487732/150‑1 (MQ=60)

                                              TCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGG                                                                                                     <  SRR19973066.2487767/150‑1 (MQ=60)

                                               CGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGT                                                                                                    >  SRR19973066.2663018/1‑150 (MQ=60)

                                                GAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTG                                                                                                   <  SRR19973066.1610141/150‑1 (MQ=60)

                                                  AGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGA                                                                                                 <  SRR19973066.1323446/150‑1 (MQ=60)

                                                    TAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAG                                                                                               <  SRR19973066.2695408/150‑1 (MQ=60)

                                                    TAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAG                                                                                               <  SRR19973066.899347/150‑1 (MQ=60)

                                                     AGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGcagg                                                                                              >  SRR19973066.2593490/1‑146 (MQ=60)

                                                     AGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGC                                                                                              <  SRR19973066.2749161/150‑1 (MQ=60)

                                                     AGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAAAGGAACATAGCGACGCAGGTGGTAGC                                                                                              >  SRR19973066.2615978/1‑150 (MQ=60)

                                                      GGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTAGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTTAAACGAAAAAAGCGACGCAGGTGGAAGCG                                                                                             >  SRR19973066.1581907/1‑150 (MQ=60)

                                                             TTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTC                                                                                      <  SRR19973066.1072435/150‑1 (MQ=60)

                                                               GCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGTAAAGGAACATCGCGACGCCGGTGGAAGCGATAGGTCGC                                                                                    >  SRR19973066.2244367/1‑150 (MQ=60)

                                                                 TGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGC                                                                                  >  SRR19973066.3204414/1‑150 (MQ=60)

                                                                  GCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCC                                                                                 >  SRR19973066.2694042/1‑150 (MQ=60)

                                                                     GTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAACGGAAAAAAGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTT                                                                              >  SRR19973066.1726264/1‑150 (MQ=60)

                                                                      TTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTG                                                                             <  SRR19973066.1175681/150‑1 (MQ=60)

                                                                      TTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTG                                                                             <  SRR19973066.1176058/150‑1 (MQ=60)

                                                                           GTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAG                                                                        <  SRR19973066.394669/150‑1 (MQ=60)

                                                                           GTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAG                                                                        <  SRR19973066.394687/150‑1 (MQ=60)

                                                                                   ACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCC                                                                <  SRR19973066.3546414/150‑1 (MQ=60)

                                                                                    CGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAACGCGACCCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCA                                                               >  SRR19973066.441109/1‑150 (MQ=60)

                                                                                    CGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAAACGAACAAACCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCA                                                               >  SRR19973066.441103/1‑150 (MQ=60)

                                                                                         TGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTT                                                          <  SRR19973066.1613166/150‑1 (MQ=60)

                                                                                         TGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTT                                                          <  SRR19973066.1613699/150‑1 (MQ=60)

                                                                                         TGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTT                                                          <  SRR19973066.1613750/150‑1 (MQ=60)

                                                                                          GTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTC                                                         <  SRR19973066.2809010/150‑1 (MQ=60)

                                                                                               GGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAA                                                    >  SRR19973066.2371033/1‑150 (MQ=60)

                                                                                               GGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAA                                                    >  SRR19973066.2309565/1‑150 (MQ=60)

                                                                                                GTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAG                                                   <  SRR19973066.765145/150‑1 (MQ=60)

                                                                                                  CAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTT                                                 <  SRR19973066.1491975/150‑1 (MQ=60)

                                                                                                   AAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTC                                                >  SRR19973066.2088229/1‑150 (MQ=60)

                                                                                                   AAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTC                                                >  SRR19973066.884185/1‑150 (MQ=60)

                                                                                                    AAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCC                                               <  SRR19973066.3405607/150‑1 (MQ=60)

                                                                                                     AAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCCGCGTTCGGCAAGTTCCG                                              >  SRR19973066.2938421/1‑150 (MQ=60)

                                                                                                      AATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGG                                             <  SRR19973066.2386903/150‑1 (MQ=60)

                                                                                                      AATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGG                                             <  SRR19973066.3549263/150‑1 (MQ=60)

                                                                                                      AATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGG                                             <  SRR19973066.2386932/150‑1 (MQ=60)

                                                                                                       ATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGTAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGC                                            >  SRR19973066.1423936/1‑150 (MQ=60)

                                                                                                             TGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCCAGTTCCGGCATCAGG                                      >  SRR19973066.1753319/1‑150 (MQ=60)

                                                                                                             TGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGG                                      >  SRR19973066.1754000/1‑150 (MQ=60)

                                                                                                                 AGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTTAAAGGAACATCGCGACGCACGTGGAAGCGATAGGTCGCGCCCGTGTCGAGTACATCCCAGCTTTTGGCAAGTTCGGGCATCAGGggct                                  >  SRR19973066.927062/1‑146 (MQ=60)

                                                                                                                      AACACCACATAGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA                             <  SRR19973066.2877650/150‑1 (MQ=60)

                                                                                                                         ACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAACGAACAAAGCGACCCAGGTGGAAGCGCTAGGTCGCGCCGTTTTCGAGTAATTACCAGCATTCGGCAAGTTCCCGCATCACGCGATAGGTATAG                          >  SRR19973066.2148447/1‑150 (MQ=60)

                                                                                                                         ACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAACGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTAGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGCTAGGTATAG                          >  SRR19973066.1705219/1‑150 (MQ=60)

                                                                                                                         ACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAAAATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTACCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAG                          >  SRR19973066.1705633/1‑150 (MQ=60)

                                                                                                                          CCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGG                         <  SRR19973066.1763440/150‑1 (MQ=60)

                                                                                                                            ACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGA                       <  SRR19973066.1031567/150‑1 (MQ=60)

                                                                                                                             CATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGAT                      <  SRR19973066.1730144/150‑1 (MQ=60)

                                                                                                                             CATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGAT                      <  SRR19973066.3504589/150‑1 (MQ=60)

                                                                                                                             CATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGAT                      <  SRR19973066.1828808/150‑1 (MQ=60)

                                                                                                                              ATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAAGGAACAAAGCGAACCAGGTGGAATCGATAGGTCGCGCCCGTGTCGAGTACTTACCAGCTTTAGGCAAGTTCCCGCATCAGGCGctatgtctatggatc                     >  SRR19973066.2793645/1‑135 (MQ=60)

                                                                                                                              ATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGGTTTTTtaaaaggaaaaaagccaaccacgtggtatcgataggtagcgcccttgtcgagtacataccaccattcggcaacttacggccacacccgctaggtatagtgatc                     >  SRR19973066.700716/1‑47 (MQ=60)

                                                                                                                                 GTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGAC                  <  SRR19973066.1451845/150‑1 (MQ=60)

                                                                                                                                   CGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACAT                <  SRR19973066.2082309/150‑1 (MQ=60)

                                                                                                                                    GGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAACGCGAAGCAGGTGGAAGCGAAAGGTAGCGCCGTTGTCCAGTACTTCCCACCATTCGGCAACATCCCGCATCAGGCGATAGGTATAGGGATCGACATC               >  SRR19973066.3219180/1‑150 (MQ=60)

                                                                                                                                     GCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAAGGAACAACGCGACGCAGGTGGAAACGATATGTCGCGCCGTTGTAGAGTACGTCCCAGCTTTCGGCAAGTTCAGGCAAAAGGCGATAGGTATAGGGATCGACAaac              >  SRR19973066.2571440/1‑147 (MQ=60)

                                                                                                                                     GCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAAAGCGACGCAGGTGGAATCGATAGGTCGCGCCGGTGTCGAGTAATTACCAGCATTAGGCAAGTTACGGCATCAGGCGATAGGTATATGGATCGAAATCC              >  SRR19973066.2571525/1‑150 (MQ=60)

                                                                                                                                      CATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGTAACGGAACAAAGCGACGCAGGTGGAAGCGATAGGTCGCGCCGGTGTCGAGTACATACCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATATGGATCGACATCCA             >  SRR19973066.2751087/1‑150 (MQ=60)

                                                                                                                                      CATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATATGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATAAGGCGATAGGTATAGGGATCGACATACA             >  SRR19973066.2751082/1‑150 (MQ=60)

                                                                                                                                       ATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTttaaaagaaaaaagcgacccacgtgggagcgcaaggtcgcgccgttttcgagtacatcccaccattaggcaacttacggcaacaggcgataggtaaagtgatagacatacac            >  SRR19973066.1537904/1‑38 (MQ=60)

                                                                                                                                       ATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTTAAAAGGAACAACGCGACGCAGGTGGAATCGAAAGGTCGCGCCGTTTTCGAGTAAATCCCACCTTTAGGCAACTTACGGCAACACGCCATAGGTATAGGGATagacaaccac            >  SRR19973066.735993/1‑140 (MQ=56)

                                                                                                                                          CATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAG         <  SRR19973066.817132/150‑1 (MQ=60)

                                                                                                                                          CATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAG         <  SRR19973066.817178/150‑1 (MQ=60)

                                                                                                                                               TACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAACGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACATCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGCTAGGTATAGGGATCGACATCCAGCAGGCGAT    >  SRR19973066.1691494/1‑150 (MQ=60)

                                                                                                                                                ACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATC   <  SRR19973066.186989/150‑1 (MQ=60)

                                                                                                                                                 CTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCA  <  SRR19973066.2817817/150‑1 (MQ=60)

                                                                                                                                                 CTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCA  <  SRR19973066.2817880/150‑1 (MQ=60)

ATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCA  >  CP009273/1350876‑1351170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`