Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,479,733 G→T 100% D806Y (GAT→TAT)  hrpA → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,479,7330GT98.8% 556.3 / ‑1.8 171D806Y (GAT→TAT) hrpAputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (76/93);  total (77/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 171 total aligned reads displayed.

ACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACGATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCAT  >  CP009273/1479590‑1479876
                                                                                                                                               |                                                                                                                                               
 cTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTa                                                                                                                                            <  2:2141007/148‑1 (MQ=255)
 cTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTa                                                                                                                                            >  1:3329464/1‑148 (MQ=255)
 cTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTa                                                                                                                                            <  1:2273743/148‑1 (MQ=255)
  tGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTaa                                                                                                                                           >  2:2472193/1‑148 (MQ=255)
     cAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAAtct                                                                                                                                        <  1:2969161/148‑1 (MQ=255)
     cAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAAtct                                                                                                                                        <  1:680281/148‑1 (MQ=255)
        aCGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCg                                                                                                                                     >  2:113184/1‑148 (MQ=255)
             tCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCTCTCGc                                                                                                                                >  1:3507489/1‑148 (MQ=255)
              cACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGcc                                                                                                                               <  1:2862521/148‑1 (MQ=255)
              cACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGcc                                                                                                                               <  1:3152647/148‑1 (MQ=255)
                cGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCAc                                                                                                                             <  1:126337/148‑1 (MQ=255)
                cGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCAc                                                                                                                             >  2:1153081/1‑148 (MQ=255)
                  cATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACtt                                                                                                                           >  2:2154265/1‑148 (MQ=255)
                    ttcttcCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCg                                                                                                                         >  2:3346826/1‑148 (MQ=255)
                     tcttcCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGa                                                                                                                        >  2:2993398/1‑148 (MQ=255)
                        tcCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAg                                                                                                                     >  2:781867/1‑148 (MQ=255)
                        tcCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAg                                                                                                                     <  2:1733892/148‑1 (MQ=255)
                         ccGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGc                                                                                                                    <  2:3010326/148‑1 (MQ=255)
                          cGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCt                                                                                                                   >  1:1081247/1‑148 (MQ=255)
                              aaaaCCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCtggtg                                                                                                               >  1:2976782/1‑148 (MQ=255)
                                  ccTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGaaa                                                                                                           >  2:2960648/1‑148 (MQ=255)
                                    tGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGaaaaa                                                                                                         <  2:1520051/148‑1 (MQ=255)
                                    tGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGaaaaa                                                                                                         >  2:2969722/1‑148 (MQ=255)
                                        aCTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTc                                                                                                     >  1:1844227/1‑148 (MQ=255)
                                        aCTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTc                                                                                                     <  2:2770959/148‑1 (MQ=255)
                                        aCTACGGGCGGAAGGAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTc                                                                                                     <  2:386391/148‑1 (MQ=255)
                                         cTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCa                                                                                                    >  2:220661/1‑148 (MQ=255)
                                          tACGGGCGGAAGTAGAAGAGCTGGAACACAACTCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAg                                                                                                   <  2:2792734/148‑1 (MQ=255)
                                           aCGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGc                                                                                                  <  1:514106/148‑1 (MQ=255)
                                                 ggAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGaa                                                                                            <  2:1136771/148‑1 (MQ=255)
                                                 ggAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGaa                                                                                            <  1:10284/148‑1 (MQ=255)
                                                 ggAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGaa                                                                                            <  1:2240739/148‑1 (MQ=255)
                                                 ggAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGaa                                                                                            <  1:297286/148‑1 (MQ=255)
                                                 ggAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGaa                                                                                            <  1:1001878/148‑1 (MQ=255)
                                                  gAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGaaa                                                                                           <  2:2346527/148‑1 (MQ=255)
                                                    aGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACg                                                                                         <  2:2960900/148‑1 (MQ=255)
                                                     gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGc                                                                                        >  2:1411324/1‑148 (MQ=255)
                                                     gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGc                                                                                        <  1:3266699/148‑1 (MQ=255)
                                                     gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGc                                                                                        <  1:3091471/148‑1 (MQ=255)
                                                       agaagaGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCt                                                                                      <  1:1943266/148‑1 (MQ=255)
                                                        gaagaGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTg                                                                                     >  1:2093966/1‑148 (MQ=255)
                                                         aagaGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGa                                                                                    >  1:2266065/1‑148 (MQ=255)
                                                          agagCTGGAACACACATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGAt                                                                                   <  2:602450/148‑1 (MQ=255)
                                                          agagCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGAt                                                                                   <  1:2993398/148‑1 (MQ=255)
                                                           gagCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGAtt                                                                                  <  1:158910/148‑1 (MQ=255)
                                                           gagCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGAtt                                                                                  <  2:3302560/148‑1 (MQ=255)
                                                             gCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTg                                                                                >  2:1649824/1‑148 (MQ=255)
                                                             gCTGGAACACAAATCACGTCGCCGCGATAGTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTg                                                                                <  1:3400358/148‑1 (MQ=255)
                                                                ggAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTc                                                                             >  2:490752/1‑148 (MQ=255)
                                                                  aaCACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACGATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCaa                                                                           <  2:3131399/148‑1 (MQ=255)
                                                                        aaTCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGa                                                                     >  2:2009525/1‑148 (MQ=255)
                                                                         aTCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaa                                                                    >  1:1442330/1‑148 (MQ=255)
                                                                         aTCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaa                                                                    >  1:2950112/1‑148 (MQ=255)
                                                                         aTCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaa                                                                    <  2:1004976/148‑1 (MQ=255)
                                                                          tCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaaa                                                                   >  1:2211770/1‑148 (MQ=255)
                                                                          tCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaaa                                                                   >  1:1182193/1‑148 (MQ=255)
                                                                           cACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaaaa                                                                  >  1:1562176/1‑148 (MQ=255)
                                                                            aCGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaaaaa                                                                 <  1:1314375/148‑1 (MQ=255)
                                                                            aCGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaaaaa                                                                 <  2:2093966/148‑1 (MQ=255)
                                                                            aCGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaaaaa                                                                 <  2:546915/148‑1 (MQ=255)
                                                                            aCGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACGATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaaaaa                                                                 >  1:31445/1‑148 (MQ=255)
                                                                             cGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaaaaaa                                                                <  2:1370917/148‑1 (MQ=255)
                                                                             cGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaaaaaa                                                                <  2:2244301/148‑1 (MQ=255)
                                                                                  ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATg                                                           >  1:2521726/1‑148 (MQ=255)
                                                                                    gcgATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGtt                                                         <  2:880463/148‑1 (MQ=255)
                                                                                     cgATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTg                                                        <  1:1201593/148‑1 (MQ=255)
                                                                                      gATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGa                                                       <  2:2976782/148‑1 (MQ=255)
                                                                                         atTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCa                                                    >  2:170612/1‑148 (MQ=255)
                                                                                         atTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCa                                                    <  2:1875727/148‑1 (MQ=255)
                                                                                            cTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAag                                                 <  2:1443798/148‑1 (MQ=255)
                                                                                               gTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAgg                                              >  1:1849483/1‑148 (MQ=255)
                                                                                                 tgatgaCGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACGGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGgc                                            >  1:2603715/1‑148 (MQ=255)
                                                                                                         aaaCGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGaaaa                                    <  1:380479/148‑1 (MQ=255)
                                                                                                            cGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAAt                                 <  2:1196377/148‑1 (MQ=255)
                                                                                                            cGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAAt                                 <  2:1196295/148‑1 (MQ=255)
                                                                                                             gttgttTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATc                                <  2:62084/148‑1 (MQ=255)
                                                                                                             gttgttTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATc                                <  2:1839993/148‑1 (MQ=255)
                                                                                                             gttgttTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATc                                <  2:2579561/148‑1 (MQ=255)
                                                                                                              ttgttTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCa                               <  1:112771/148‑1 (MQ=255)
                                                                                                              ttgttTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCa                               >  2:3179096/1‑148 (MQ=255)
                                                                                                              ttgttTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCa                               <  1:113184/148‑1 (MQ=255)
                                                                                                               tgttTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAg                              <  1:3038854/148‑1 (MQ=255)
                                                                                                               tgttTGAGTTATACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAg                              <  1:1971946/148‑1 (MQ=255)
                                                                                                                gttTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGc                             <  1:3482224/148‑1 (MQ=255)
                                                                                                                  ttGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCaa                           >  2:497486/1‑148 (MQ=255)
                                                                                                                      gTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTg                       <  1:2910364/148‑1 (MQ=255)
                                                                                                                       ttCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTgg                      >  2:1735294/1‑148 (MQ=255)
                                                                                                                       ttCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTgg                      >  2:1735286/1‑148 (MQ=255)
                                                                                                                       ttCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTgg                      >  2:1699427/1‑148 (MQ=255)
                                                                                                                        tCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGa                     <  1:3142130/148‑1 (MQ=255)
                                                                                                                            cGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAg                          <  1:2836377/139‑1 (MQ=255)
                                                                                                                            cGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAg                          >  2:2011305/1‑139 (MQ=255)
                                                                                                                            cGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAg                          >  2:2836377/1‑139 (MQ=255)
                                                                                                                             gACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTAcc                <  2:1549641/148‑1 (MQ=255)
                                                                                                                             gACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTAcc                <  2:1549463/148‑1 (MQ=255)
                                                                                                                                cAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGa             >  2:1213923/1‑148 (MQ=255)
                                                                                                                                  gcgcATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAAc           >  1:846218/1‑148 (MQ=255)
                                                                                                                                  gcgcATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAAc           <  1:3196048/148‑1 (MQ=255)
                                                                                                                                     cATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTc        >  1:2031584/1‑148 (MQ=255)
                                                                                                                                           ccACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCAt  >  2:1652401/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                               
ACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACGATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCAT  >  CP009273/1479590‑1479876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACGATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGC  >  CP009273/1479586‑1479882
                                                                                                                                                   |                                                                                                                                                     
GGTGACTGGCAGACGCGTCACGCATTCTTCCGTTAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTAT                                                                                                                                                     <  SRR19973065.2594775/150‑1 (MQ=60)
 GTGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATG                                                                                                                                                    <  SRR19973065.2524559/150‑1 (MQ=60)
  TGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGT                                                                                                                                                   >  SRR19973065.3008178/1‑150 (MQ=60)
   GACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTA                                                                                                                                                  >  SRR19973065.3349783/1‑150 (MQ=60)
     CTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAAT                                                                                                                                                <  SRR19973065.2288095/150‑1 (MQ=60)
         CAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCC                                                                                                                                            <  SRR19973065.2987319/150‑1 (MQ=60)
         CAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCC                                                                                                                                            <  SRR19973065.684810/150‑1 (MQ=60)
               CGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCTCTCGC                                                                                                                                      >  SRR19973065.3528774/1‑150 (MQ=60)
               CGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGC                                                                                                                                      >  SRR19973065.1771255/1‑150 (MQ=60)
                  CACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCAC                                                                                                                                   <  SRR19973065.1118535/150‑1 (MQ=60)
                  CACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCAC                                                                                                                                   <  SRR19973065.3171772/150‑1 (MQ=60)
                  CACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCAC                                                                                                                                   <  SRR19973065.3171588/150‑1 (MQ=60)
                  CACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCAC                                                                                                                                   <  SRR19973065.2880093/150‑1 (MQ=60)
                    CGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTT                                                                                                                                 <  SRR19973065.3203760/150‑1 (MQ=60)
                    CGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTT                                                                                                                                 <  SRR19973065.127426/150‑1 (MQ=60)
                            TCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCT                                                                                                                         >  SRR19973065.1088382/1‑150 (MQ=60)
                             CCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATTACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTG                                                                                                                        <  SRR19973065.1358528/150‑1 (MQ=60)
                             CCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTG                                                                                                                        <  SRR19973065.1077096/150‑1 (MQ=60)
                              CGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGG                                                                                                                       >  SRR19973065.3077567/1‑150 (MQ=60)
                               GTGAAACCCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGT                                                                                                                      >  SRR19973065.1146581/1‑150 (MQ=60)
                                TGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTG                                                                                                                     >  SRR19973065.2994312/1‑150 (MQ=60)
                                TGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTG                                                                                                                     >  SRR19973065.2994995/1‑150 (MQ=60)
                                    AACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAA                                                                                                                 >  SRR19973065.2755217/1‑150 (MQ=60)
                                          AAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTC                                                                                                           >  SRR19973065.1856388/1‑150 (MQ=60)
                                              TACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCC                                                                                                       >  SRR19973065.2880335/1‑150 (MQ=60)
                                               ACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCG                                                                                                      <  SRR19973065.517508/150‑1 (MQ=60)
                                                     GGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAAC                                                                                                <  SRR19973065.299205/150‑1 (MQ=60)
                                                     GGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAAC                                                                                                <  SRR19973065.10446/150‑1 (MQ=60)
                                                     GGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAAC                                                                                                <  SRR19973065.2254980/150‑1 (MQ=60)
                                                     GGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAAC                                                                                                <  SRR19973065.1008533/150‑1 (MQ=60)
                                                         GTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCT                                                                                            <  SRR19973065.3286505/150‑1 (MQ=60)
                                                         GTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCT                                                                                            <  SRR19973065.3110348/150‑1 (MQ=60)
                                                         GTAGAAGAGCTGGAACACAAATCACGTCGCCGAGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCT                                                                                            <  SRR19973065.3287837/150‑1 (MQ=60)
                                                          TAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTG                                                                                           >  SRR19973065.2107418/1‑150 (MQ=60)
                                                           AGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGA                                                                                          >  SRR19973065.2280387/1‑150 (MQ=60)
                                                           AGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGA                                                                                          <  SRR19973065.1955971/150‑1 (MQ=60)
                                                           AGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGA                                                                                          <  SRR19973065.1955968/150‑1 (MQ=60)
                                                              AGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTT                                                                                       <  SRR19973065.3011730/150‑1 (MQ=60)
                                                              AGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTT                                                                                       >  SRR19973065.2431773/1‑150 (MQ=60)
                                                               GAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTG                                                                                      <  SRR19973065.160224/150‑1 (MQ=60)
                                                                 GCTGGAACACAAATCACGTCGCCGCGATAGTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCT                                                                                    <  SRR19973065.3421142/150‑1 (MQ=60)
                                                                      AACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACT                                                                               <  SRR19973065.927462/150‑1 (MQ=60)
                                                                         ACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTG                                                                            >  SRR19973065.2595645/1‑150 (MQ=60)
                                                                           AAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAA                                                                          >  SRR19973065.1285169/1‑150 (MQ=60)
                                                                           AAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAA                                                                          >  SRR19973065.1451669/1‑150 (MQ=60)
                                                                           AAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAA                                                                          >  SRR19973065.2967533/1‑150 (MQ=60)
                                                                           AAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAA                                                                          >  SRR19973065.2968077/1‑150 (MQ=60)
                                                                            AATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAA                                                                         >  SRR19973065.1189821/1‑150 (MQ=60)
                                                                            AATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAA                                                                         >  SRR19973065.1189678/1‑150 (MQ=60)
                                                                            AATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAA                                                                         >  SRR19973065.2225908/1‑150 (MQ=60)
                                                                             ATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAA                                                                        >  SRR19973065.1572557/1‑150 (MQ=60)
                                                                              TCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACGATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAA                                                                       >  SRR19973065.31840/1‑150 (MQ=60)
                                                                               CACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAA                                                                      >  SRR19973065.3149165/1‑150 (MQ=60)
                                                                                ACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAG                                                                     <  SRR19973065.1323067/150‑1 (MQ=60)
                                                                                    CGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATG                                                                 >  SRR19973065.2537561/1‑150 (MQ=60)
                                                                                      CCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTT                                                               <  SRR19973065.787053/150‑1 (MQ=60)
                                                                                      CCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTT                                                               <  SRR19973065.3367275/150‑1 (MQ=60)
                                                                                         CGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGAT                                                            <  SRR19973065.1209286/150‑1 (MQ=60)
                                                                                             ATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGCCCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAA                                                        <  SRR19973065.436971/150‑1 (MQ=60)
                                                                                                 TGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGG                                                    >  SRR19973065.1861628/1‑150 (MQ=60)
                                                                                                 TGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGG                                                    >  SRR19973065.1861674/1‑150 (MQ=60)
                                                                                                   GTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACGGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGC                                                  >  SRR19973065.2619881/1‑150 (MQ=60)
                                                                                                     TGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGC                                                >  SRR19973065.369734/1‑150 (MQ=60)
                                                                                                             AAACGTTGTTTTAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAA                                        <  SRR19973065.383143/150‑1 (MQ=60)
                                                                                                              AACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAAT                                       <  SRR19973065.1456900/150‑1 (MQ=60)
                                                                                                                  TTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGC                                   <  SRR19973065.114192/150‑1 (MQ=60)
                                                                                                                  TTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGC                                   <  SRR19973065.113777/150‑1 (MQ=60)
                                                                                                                   TGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCA                                  <  SRR19973065.3057461/150‑1 (MQ=60)
                                                                                                                   TGTTTGAGTTATACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCA                                  <  SRR19973065.1984795/150‑1 (MQ=60)
                                                                                                                    GTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAA                                 <  SRR19973065.3503412/150‑1 (MQ=60)
                                                                                                                     TTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAG                                <  SRR19973065.3454783/150‑1 (MQ=60)
                                                                                                                       ccgatctTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCT                              <  SRR19973065.2853841/143‑1 (MQ=60)
                                                                                                                       ccgatctTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCT                              <  SRR19973065.2024279/143‑1 (MQ=60)
                                                                                                                         AGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGG                            >  SRR19973065.2228841/1‑150 (MQ=60)
                                                                                                                          GTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGA                           <  SRR19973065.2928082/150‑1 (MQ=60)
                                                                                                                            TCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATT                         <  SRR19973065.3161225/150‑1 (MQ=60)
                                                                                                                                    CAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAAC                 >  SRR19973065.851706/1‑150 (MQ=60)
                                                                                                                                    CAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAAC                 >  SRR19973065.2277864/1‑150 (MQ=60)
                                                                                                                                     AGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACT                <  SRR19973065.2987883/150‑1 (MQ=60)
                                                                                                                                      GCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTT               <  SRR19973065.3215308/150‑1 (MQ=60)
                                                                                                                                       CGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTC              >  SRR19973065.2044754/1‑150 (MQ=60)
                                                                                                                                       CGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTC              >  SRR19973065.2044624/1‑150 (MQ=60)
                                                                                                                                                CACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAG     >  SRR19973065.3433628/1‑150 (MQ=60)
                                                                                                                                                CACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAG     >  SRR19973065.3433627/1‑150 (MQ=60)
                                                                                                                                                 ACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGG    <  SRR19973065.1565573/150‑1 (MQ=60)
                                                                                                                                                 ACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGG    <  SRR19973065.2978725/150‑1 (MQ=60)
                                                                                                                                                   TATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGC  <  SRR19973065.1367396/150‑1 (MQ=60)
                                                                                                                                                   TATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGC  >  SRR19973065.2777481/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
GGTGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACGATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGC  >  CP009273/1479586‑1479882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑