Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,480,656 C→A 20.1% I1113I (ATC→ATA)  hrpA → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,480,6560CA20.1% 467.1 / 73.6 178I1113I (ATC→ATA) hrpAputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base C (86/56);  new base A (20/16);  total (106/72)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 187 total aligned reads displayed.

GGCAATGTGGAACGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGC  >  CP009273/1480509‑1480802
                                                                                                                                                   |                                                                                                                                                  
ggCAATGTGGAACGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTAtc                                                                                                                                                    >  2:3497238/1‑148 (MQ=255)
 gCAATGTGGAACGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTAtct                                                                                                                                                   >  1:2846931/1‑148 (MQ=255)
  cAATGTGGAACGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTAtctc                                                                                                                                                  >  1:2518321/1‑148 (MQ=255)
    aTGTGGAACGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCt                                                                                                                                                >  1:2448133/1‑148 (MQ=255)
    aTGTGGAACGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCt                                                                                                                                                <  2:1541635/148‑1 (MQ=255)
          aaCGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGgtg                                                                                                                                          <  2:2216832/148‑1 (MQ=255)
             ggTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTgg                                                                                                                                       <  2:190844/148‑1 (MQ=255)
             ggTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTgg                                                                                                                                       <  1:2959479/148‑1 (MQ=255)
              gTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATATCCTGCGGTGTGGa                                                                                                                                      <  1:394512/148‑1 (MQ=255)
                cTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATa                                                                                                                                    >  1:2475842/1‑148 (MQ=255)
                 ttCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATaa                                                                                                                                   <  1:1832241/148‑1 (MQ=255)
                 ttCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATATCCTGCGGTGTGGATaa                                                                                                                                   <  1:2878167/148‑1 (MQ=255)
                 ttCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATATCCTGCGGTGTGGATaa                                                                                                                                   <  1:518772/148‑1 (MQ=255)
                   cGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAAt                                                                                                                                 <  1:3517271/148‑1 (MQ=255)
                   cGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAAt                                                                                                                                 <  1:3517161/148‑1 (MQ=255)
                      cGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGa                                                                                                                              <  1:3481361/148‑1 (MQ=255)
                          tACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGa                                                                                                                          <  2:1838275/148‑1 (MQ=255)
                             tgctgctgAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGc                                                                                                                       >  1:1182427/1‑148 (MQ=255)
                               ctgctgAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCa                                                                                                                     <  2:829004/148‑1 (MQ=255)
                                 gctgAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAAt                                                                                                                   <  2:142397/148‑1 (MQ=255)
                                  ctgAATATTCCATCGCCAATCAAATATTTACATGAAAATTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAAtg                                                                                                                  <  2:3271532/148‑1 (MQ=255)
                                  ctgAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAAtg                                                                                                                  <  2:3271509/148‑1 (MQ=255)
                                   tgAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAAtgg                                                                                                                 >  1:881439/1‑148 (MQ=255)
                                    gAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAAtggt                                                                                                                >  1:2964136/1‑148 (MQ=255)
                                    gAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAAtggt                                                                                                                >  1:3327206/1‑148 (MQ=255)
                                     aaTATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAATCCAAGCTGTGACTGTACTTTAACCCGTATGGCAAAGATCTGGAGCTGATCGACGACTTTATCTACTGCGGTTTGGATAAATTGATCTACGCCAAtggtg                                                                                                               >  2:2201158/1‑148 (MQ=255)
                                     aaTATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATATCCTGCGGTGTGGATAAATTGATCGACGCCAAtgttg                                                                                                               >  1:3008813/1‑148 (MQ=255)
                                      atatTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAAtggtgg                                                                                                              >  2:2903630/1‑148 (MQ=255)
                                      atatTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATATCCTGCGGTGTGGATAAATTGATCGACGCCAAtggtgg                                                                                                              <  1:3172707/148‑1 (MQ=255)
                                        atTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGcc                                                                                                            >  1:267983/1‑148 (MQ=255)
                                          tCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATATCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCg                                                                                                          >  2:651506/1‑148 (MQ=255)
                                           ccATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCgg                                                                                                         >  2:663698/1‑148 (MQ=255)
                                             aTCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTc                                                                                                       >  1:2409839/1‑148 (MQ=255)
                                             aTCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTc                                                                                                       >  1:2409783/1‑148 (MQ=255)
                                              tCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATATCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCt                                                                                                      >  2:1215152/1‑148 (MQ=255)
                                                gCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTgg                                                                                                    <  1:3581097/148‑1 (MQ=255)
                                                   aaTCAAATATTTACATGAACAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACg                                                                                                 <  1:73017/148‑1 (MQ=255)
                                                   aaTCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATAGATAGACGCCAATGGTGGCCCGGTCTGGACg                                                                                                 >  1:3331099/1‑148 (MQ=255)
                                                    aTCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATATCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACgg                                                                                                >  1:3164409/1‑148 (MQ=255)
                                                      cAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGgaa                                                                                              >  1:2666249/1‑148 (MQ=255)
                                                          tatTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGaagaag                                                                                          >  1:938004/1‑148 (MQ=255)
                                                            tttACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGTCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGc                                                                                        <  1:3110041/148‑1 (MQ=255)
                                                            tttACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATATCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGc                                                                                        <  2:1923470/148‑1 (MQ=255)
                                                             ttACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCt                                                                                       >  1:1746108/1‑148 (MQ=255)
                                                              tACATGAAAAGTTACCGAACAAAGCCACGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCtt                                                                                      <  2:3534157/148‑1 (MQ=255)
                                                                 aTGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTtgc                                                                                   >  2:1698587/1‑148 (MQ=255)
                                                                   gAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTtgctg                                                                                 <  2:3297466/148‑1 (MQ=255)
                                                                   gAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTtgctg                                                                                 <  2:3297484/148‑1 (MQ=255)
                                                                    aaaaGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTtgctgc                                                                                >  1:1645296/1‑148 (MQ=255)
                                                                    aaaaGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTtgctgc                                                                                >  1:503668/1‑148 (MQ=255)
                                                                    aaaaGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATATCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTtgctgc                                                                                >  2:3762280/1‑148 (MQ=255)
                                                                        gTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTgctgcgctg                                                                            >  2:1326380/1‑148 (MQ=255)
                                                                         ttACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTgctgcgctgc                                                                           >  1:1556614/1‑148 (MQ=255)
                                                                           aCCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATATCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCAt                                                                         >  2:1029342/1‑148 (MQ=255)
                                                                                aCAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCTGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGaaaa                                                                    >  1:1922163/1‑148 (MQ=255)
                                                                                aCAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGaaaa                                                                    >  2:3024165/1‑148 (MQ=255)
                                                                                 cAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATATCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGaaaaa                                                                   >  2:1363112/1‑148 (MQ=255)
                                                                                  aaaGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAACAAg                                                                  >  2:3720710/1‑148 (MQ=255)
                                                                                   aaGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAgt                                                                 >  2:3164017/1‑148 (MQ=255)
                                                                                      ccAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCtt                                                                                      <  1:2148969/124‑1 (MQ=255)
                                                                                      ccAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAgtgcg                                                              >  2:3369649/1‑148 (MQ=255)
                                                                                            tGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGa                                                        >  1:1254840/1‑148 (MQ=255)
                                                                                                 cTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGa                                                   <  1:3724738/148‑1 (MQ=255)
                                                                                                    tACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACg                                                >  2:2410288/1‑148 (MQ=255)
                                                                                                        ttAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGacac                                            >  2:2041440/1‑148 (MQ=255)
                                                                                                          aaCCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACgg                                          <  2:3674608/148‑1 (MQ=255)
                                                                                                            cccGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACggtg                                        >  1:3282808/1‑148 (MQ=255)
                                                                                                            cccGTATGGCAAAGTGCTGGAGATTATCGACGACTGTATCTCCTGCGGTGTGTATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACggtg                                        <  1:1666074/148‑1 (MQ=255)
                                                                                                             ccGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACggtgg                                       >  2:429631/1‑148 (MQ=255)
                                                                                                                 aTGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGa                                   <  1:864784/148‑1 (MQ=255)
                                                                                                                  tgtcacaGTGCTGGAGCTGATCGAGGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCTAACTGAACGACACGGTGGTGGat                                  <  1:595319/142‑1 (MQ=255)
                                                                                                                  tGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGAAGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGat                                  <  1:3272384/148‑1 (MQ=255)
                                                                                                                   ggCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGata                                 >  1:680157/1‑148 (MQ=255)
                                                                                                                   ggCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGata                                 >  1:2689518/1‑148 (MQ=255)
                                                                                                                    gCAAAGTGCTGGAGCTGATCGACGACTGTATATCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGatat                                <  1:242993/148‑1 (MQ=255)
                                                                                                                     cAAAGTGCTGGAGCTGATCGACGACTGTATATCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATAtt                               <  1:1363112/148‑1 (MQ=255)
                                                                                                                       aaGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGc                             >  2:633031/1‑148 (MQ=255)
                                                                                                                        aGTGCTGGAGCAGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACTCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATAGCg                            >  2:2470969/1‑148 (MQ=255)
                                                                                                                         gTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGa                           >  2:1084342/1‑148 (MQ=255)
                                                                                                                         gTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGa                           <  1:663698/148‑1 (MQ=255)
                                                                                                                         gTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGa                           >  2:409542/1‑148 (MQ=255)
                                                                                                                             tGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCa                       <  1:1967271/148‑1 (MQ=255)
                                                                                                                                aGCTGATCGACGACTGTATATCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGt                    <  2:1338072/148‑1 (MQ=255)
                                                                                                                                 gCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTc                   <  2:2488654/148‑1 (MQ=255)
                                                                                                                                  cTGATCGACGACTGTATATCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCg                  >  1:3311664/1‑148 (MQ=255)
                                                                                                                                    gATCGACGACTGTATATCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAg                >  1:511309/1‑148 (MQ=255)
                                                                                                                                     aTCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGc               >  1:441164/1‑148 (MQ=255)
                                                                                                                                       cgacgaCTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCaa             <  1:1637169/148‑1 (MQ=255)
                                                                                                                                        gacgacTGTATATCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCaaa            <  2:1595860/148‑1 (MQ=255)
                                                                                                                                          cgacTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATc          >  1:996766/1‑148 (MQ=255)
                                                                                                                                           gacTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATcc         <  1:1252176/148‑1 (MQ=255)
                                                                                                                                            acTGTATATCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCt        >  2:2605896/1‑148 (MQ=255)
                                                                                                                                             cTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCtt       >  1:3138972/1‑148 (MQ=255)
                                                                                                                                             cTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCtt       >  2:896050/1‑148 (MQ=255)
                                                                                                                                             cTGTATATCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCtt       <  2:1965149/148‑1 (MQ=255)
                                                                                                                                                 aTCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACgg   >  1:3707549/1‑148 (MQ=255)
                                                                                                                                                 aTCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACgg   >  1:1381123/1‑148 (MQ=255)
                                                                                                                                                  tctcCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGc  >  2:1506991/1‑148 (MQ=255)
                                                                                                                                                  tctcCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGc  >  2:439148/1‑148 (MQ=255)
                                                                                                                                                  tctcCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGc  >  1:3748561/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCAATGTGGAACGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGC  >  CP009273/1480509‑1480802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A