Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,479,733 G→T D806Y (GAT→TAT)  hrpA → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,479,7330GT99.5% 700.3 / NA 213D806Y (GAT→TAT) hrpAputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (106/106);  total (107/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

GGTGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACGATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAG  >  CP009273/1479586‑1479879
                                                                                                                                                   |                                                                                                                                                  
ggTGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTAt                                                                                                                                                  <  2:2390345/150‑1 (MQ=255)
 gTGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATg                                                                                                                                                 >  2:3248960/1‑150 (MQ=255)
 gTGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATg                                                                                                                                                 <  2:1921889/150‑1 (MQ=255)
  tGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGt                                                                                                                                                >  1:725639/1‑150 (MQ=255)
      tGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAAtc                                                                                                                                            <  1:2688493/150‑1 (MQ=255)
       ggCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAAtct                                                                                                                                           <  1:2950300/150‑1 (MQ=255)
       ggCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAAtct                                                                                                                                           <  1:2630824/150‑1 (MQ=255)
        gCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAAtctc                                                                                                                                          <  2:3478629/150‑1 (MQ=255)
        ccAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAAtctc                                                                                                                                          >  2:3214625/2‑150 (MQ=255)
          aGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCg                                                                                                                                        >  2:245969/1‑150 (MQ=255)
          aGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCg                                                                                                                                        >  2:1984107/1‑150 (MQ=255)
          aGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCg                                                                                                                                        <  1:2896006/150‑1 (MQ=255)
             cgcgTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTc                                                                                                                                     <  1:2971982/150‑1 (MQ=255)
               cgTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGc                                                                                                                                   >  1:2508677/1‑150 (MQ=255)
                  cACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCAc                                                                                                                                >  1:2471534/1‑150 (MQ=255)
                    cGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACtt                                                                                                                              >  1:3579301/1‑150 (MQ=255)
                     gCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTc                                                                                                                             >  2:3253758/1‑150 (MQ=255)
                     gCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTc                                                                                                                             <  2:3737301/150‑1 (MQ=255)
                      cATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCg                                                                                                                            >  1:179135/1‑150 (MQ=255)
                       aTTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGa                                                                                                                           <  1:942701/150‑1 (MQ=255)
                       aTTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGa                                                                                                                           >  1:117760/1‑150 (MQ=255)
                          cttcCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAg                                                                                                                        >  1:593891/1‑150 (MQ=255)
                          cttcCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAg                                                                                                                        >  2:3421977/1‑150 (MQ=255)
                          cttcCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAg                                                                                                                        >  2:3422265/1‑150 (MQ=255)
                          cttcCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAg                                                                                                                        >  2:1488525/1‑150 (MQ=255)
                           ttcCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGc                                                                                                                       >  1:310718/1‑150 (MQ=255)
                                tGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCtggtg                                                                                                                  >  2:1728180/1‑150 (MQ=255)
                                   aaaCCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGaa                                                                                                               <  2:2757281/150‑1 (MQ=255)
                                     aCCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGTGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGaaaa                                                                                                             >  2:212342/1‑150 (MQ=255)
                                            aCTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAg                                                                                                      <  2:3082703/150‑1 (MQ=255)
                                               aCGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCcg                                                                                                   <  2:525779/150‑1 (MQ=255)
                                                cGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCcgc                                                                                                  >  1:3250350/1‑150 (MQ=255)
                                                     ggAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAAc                                                                                             >  2:1490195/1‑150 (MQ=255)
                                                     ggAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAAc                                                                                             >  1:2730318/1‑150 (MQ=255)
                                                     ggAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAAc                                                                                             >  2:2744212/1‑150 (MQ=255)
                                                     ggAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAAc                                                                                             >  2:1583540/1‑150 (MQ=255)
                                                      gAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACg                                                                                            <  1:1281326/150‑1 (MQ=255)
                                                        aGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGATGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACATCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGcc                                                                                          >  1:2872129/1‑150 (MQ=255)
                                                          tAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTg                                                                                        <  1:2540203/150‑1 (MQ=255)
                                                          tAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTg                                                                                        >  1:201869/1‑150 (MQ=255)
                                                            gaagaGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGAt                                                                                      <  2:1862688/150‑1 (MQ=255)
                                                              agagCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGAttt                                                                                    <  1:1372160/150‑1 (MQ=255)
                                                               gagCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTg                                                                                   <  1:1706763/150‑1 (MQ=255)
                                                                    ggAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCaa                                                                              <  2:3584212/150‑1 (MQ=255)
                                                                     gAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAAc                                                                             >  1:19244/1‑150 (MQ=255)
                                                                      aaCACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACt                                                                            >  1:627977/1‑150 (MQ=255)
                                                                      aaCACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACt                                                                            <  1:2348178/150‑1 (MQ=255)
                                                                       acacAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACtt                                                                           <  2:227769/150‑1 (MQ=255)
                                                                       acacAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACtt                                                                           >  2:3128818/1‑150 (MQ=255)
                                                                            aaTCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaaa                                                                      <  2:411498/150‑1 (MQ=255)
                                                                            aaTCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaaa                                                                      >  1:2861019/1‑150 (MQ=255)
                                                                              tCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaaaaa                                                                    >  1:2340772/1‑150 (MQ=255)
                                                                               cACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaaaaaa                                                                   >  2:3329750/1‑150 (MQ=255)
                                                                               cACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaaaaaa                                                                   >  2:103520/1‑150 (MQ=255)
                                                                                  gTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCa                                                                >  1:1305621/1‑150 (MQ=255)
                                                                                   tCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCAt                                                               <  1:1700895/150‑1 (MQ=255)
                                                                                     gccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGt                                                             >  2:3721699/1‑150 (MQ=255)
                                                                                      ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGtt                                                            >  2:2947572/1‑150 (MQ=255)
                                                                                       cgcgATATTCTGGTTGATGACGAAACGTTGTGTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTg                                                           <  1:808856/150‑1 (MQ=255)
                                                                                        gcgATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGa                                                          <  1:3329750/150‑1 (MQ=255)
                                                                                         cgATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGAt                                                         >  2:656224/1‑150 (MQ=255)
                                                                                         cgATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGAt                                                         <  1:1490123/150‑1 (MQ=255)
                                                                                          gATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATc                                                        >  1:853933/1‑150 (MQ=255)
                                                                                           atatTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCa                                                       >  2:2807446/1‑150 (MQ=255)
                                                                                             atTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCaaa                                                     <  2:55880/150‑1 (MQ=255)
                                                                                                 tGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAgg                                                 >  2:1697582/1‑150 (MQ=255)
                                                                                                    ttGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGgcg                                              <  2:3538846/150‑1 (MQ=255)
                                                                                                    ttGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGgcg                                              <  2:2858932/150‑1 (MQ=255)
                                                                                                        tgaCGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGa                                          <  1:673655/150‑1 (MQ=255)
                                                                                                            gAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGaaaaa                                      >  1:1814460/1‑150 (MQ=255)
                                                                                                            gAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGaaaaa                                      <  2:3390638/150‑1 (MQ=255)
                                                                                                             aaaCGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGaaaaaa                                     >  2:1333249/1‑150 (MQ=255)
                                                                                                                ctttgttTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCa                                  >  2:919723/3‑150 (MQ=255)
                                                                                                                cGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCa                                  <  1:3295503/150‑1 (MQ=255)
                                                                                                                 gttgttTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCTCTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAg                                 <  1:2350191/150‑1 (MQ=255)
                                                                                                                 gttgttTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCTAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAg                                 <  1:3214181/150‑1 (MQ=255)
                                                                                                                  ttgttTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGc                                <  1:1503438/150‑1 (MQ=255)
                                                                                                                    gttTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCaa                              <  1:1876287/150‑1 (MQ=255)
                                                                                                                       tGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCt                           <  1:487283/150‑1 (MQ=255)
                                                                                                                       tGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCt                           <  2:2591563/150‑1 (MQ=255)
                                                                                                                       tGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCt                           <  1:2533506/150‑1 (MQ=255)
                                                                                                                       tGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCt                           <  2:576468/150‑1 (MQ=255)
                                                                                                                        gAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTg                          >  1:2388454/1‑150 (MQ=255)
                                                                                                                                cGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTAccc                  >  2:747063/1‑150 (MQ=255)
                                                                                                                                cGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTAccc                  >  1:1779375/1‑150 (MQ=255)
                                                                                                                                cGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTAccc                  >  2:2888957/1‑150 (MQ=255)
                                                                                                                                   ccAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGaa               >  2:1158770/1‑150 (MQ=255)
                                                                                                                                   ccAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGaa               >  1:2749346/1‑150 (MQ=255)
                                                                                                                                    cAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAAc              <  2:828720/150‑1 (MQ=255)
                                                                                                                                    cAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAAc              >  1:3502354/1‑150 (MQ=255)
                                                                                                                                      gcgcATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACtt            <  2:2388454/150‑1 (MQ=255)
                                                                                                                                        gcATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCt          >  2:1606901/1‑150 (MQ=255)
                                                                                                                                         cATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTg         >  2:2233700/1‑150 (MQ=255)
                                                                                                                                         cATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTg         >  1:2206608/1‑150 (MQ=255)
                                                                                                                                            cAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCa      >  1:3486971/1‑150 (MQ=255)
                                                                                                                                            cAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCa      >  2:1834917/1‑150 (MQ=255)
                                                                                                                                            cAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCa      >  1:18938/1‑150 (MQ=255)
                                                                                                                                            cAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCa      <  2:2872129/150‑1 (MQ=255)
                                                                                                                                            cAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCa      <  2:1237630/150‑1 (MQ=255)
                                                                                                                                             aGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCAt     <  1:313800/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGTGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACGATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAG  >  CP009273/1479586‑1479879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGGTGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACGATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGG  >  CP009273/1479584‑1479880
                                                                                                                                                     |                                                                                                                                                   
AAGGTGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACT                                                                                                                                                     >  SRR19973061.2342012/1‑150 (MQ=60)
 AGGTGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTA                                                                                                                                                    >  SRR19973061.879664/1‑150 (MQ=60)
   GTGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATG                                                                                                                                                  >  SRR19973061.3331241/1‑150 (MQ=60)
    TGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGT                                                                                                                                                 >  SRR19973061.727535/1‑150 (MQ=60)
        TGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATC                                                                                                                                             <  SRR19973061.2695470/150‑1 (MQ=60)
        TGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATC                                                                                                                                             <  SRR19973061.2695408/150‑1 (MQ=60)
         GGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCT                                                                                                                                            <  SRR19973061.2957985/150‑1 (MQ=60)
         GGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCT                                                                                                                                            <  SRR19973061.2957905/150‑1 (MQ=60)
         GGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCT                                                                                                                                            <  SRR19973061.2637583/150‑1 (MQ=60)
            AGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCG                                                                                                                                         <  SRR19973061.2903437/150‑1 (MQ=60)
              ACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCT                                                                                                                                       <  SRR19973061.2351296/150‑1 (MQ=60)
               CGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTC                                                                                                                                      <  SRR19973061.2979644/150‑1 (MQ=60)
                 CGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGC                                                                                                                                    >  SRR19973061.2515069/1‑150 (MQ=60)
                   TCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCA                                                                                                                                  <  SRR19973061.1821135/150‑1 (MQ=60)
                   TCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCA                                                                                                                                  <  SRR19973061.2327512/150‑1 (MQ=60)
                    CACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCAC                                                                                                                                 >  SRR19973061.2477829/1‑150 (MQ=60)
                      CGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTT                                                                                                                               >  SRR19973061.3588477/1‑150 (MQ=60)
                      CGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTT                                                                                                                               >  SRR19973061.643227/1‑150 (MQ=60)
                        CATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCG                                                                                                                             >  SRR19973061.179679/1‑150 (MQ=60)
                        CATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCG                                                                                                                             >  SRR19973061.877079/1‑150 (MQ=60)
                         ATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGA                                                                                                                            >  SRR19973061.118147/1‑150 (MQ=60)
                         ATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGA                                                                                                                            <  SRR19973061.945092/150‑1 (MQ=60)
                            CTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAG                                                                                                                         >  SRR19973061.595439/1‑150 (MQ=60)
                             TTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGC                                                                                                                        >  SRR19973061.311534/1‑150 (MQ=60)
                              TCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCT                                                                                                                       <  SRR19973061.3503384/150‑1 (MQ=60)
                                   GAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGG                                                                                                                  <  SRR19973061.1882538/150‑1 (MQ=60)
                                   GAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGG                                                                                                                  >  SRR19973061.2941271/1‑150 (MQ=60)
                                     AAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAA                                                                                                                <  SRR19973061.31431/150‑1 (MQ=60)
                                             AACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCA                                                                                                        >  SRR19973061.1761249/1‑150 (MQ=60)
                                             AACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCA                                                                                                        >  SRR19973061.2391810/1‑150 (MQ=60)
                                                 ACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCG                                                                                                    >  SRR19973061.2678486/1‑150 (MQ=60)
                                                  CGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGC                                                                                                   >  SRR19973061.3258711/1‑150 (MQ=60)
                                                   GGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCG                                                                                                  <  SRR19973061.3396980/150‑1 (MQ=60)
                                                       GGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAAC                                                                                              >  SRR19973061.2737339/1‑150 (MQ=60)
                                                       GGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAAC                                                                                              >  SRR19973061.826892/1‑150 (MQ=60)
                                                        GAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACG                                                                                             <  SRR19973061.1284489/150‑1 (MQ=60)
                                                          AGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCC                                                                                           >  SRR19973061.2879482/1‑150 (MQ=60)
                                                          AGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCC                                                                                           >  SRR19973061.1240705/1‑150 (MQ=60)
                                                          AGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGATGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACATCGACAGCTGGTGGAAAAAAGTCAGCCGCGAACCGCC                                                                                           >  SRR19973061.2879493/1‑150 (MQ=60)
                                                            TAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTG                                                                                         <  SRR19973061.2546669/150‑1 (MQ=60)
                                                            TAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTG                                                                                         >  SRR19973061.202471/1‑150 (MQ=60)
                                                             AGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGA                                                                                        >  SRR19973061.2598548/1‑150 (MQ=60)
                                                             AGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGA                                                                                        >  SRR19973061.2598213/1‑150 (MQ=60)
                                                                AGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTT                                                                                     <  SRR19973061.1375627/150‑1 (MQ=60)
                                                                 GAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTG                                                                                    <  SRR19973061.1711028/150‑1 (MQ=60)
                                                                 GAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTG                                                                                    <  SRR19973061.1711047/150‑1 (MQ=60)
                                                                  AGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGC                                                                                   <  SRR19973061.206866/150‑1 (MQ=60)
                                                                  AGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGC                                                                                   <  SRR19973061.129231/150‑1 (MQ=60)
                                                                     TGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCA                                                                                <  SRR19973061.3447081/150‑1 (MQ=60)
                                                                      GGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAA                                                                               >  SRR19973061.2250738/1‑150 (MQ=60)
                                                                       GAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAAC                                                                              >  SRR19973061.1935987/1‑150 (MQ=60)
                                                                       GAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAAC                                                                              >  SRR19973061.19321/1‑150 (MQ=60)
                                                                       GAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAAC                                                                              <  SRR19973061.2754392/150‑1 (MQ=60)
                                                                        AACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACT                                                                             >  SRR19973061.629578/1‑150 (MQ=60)
                                                                        AACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACT                                                                             <  SRR19973061.2354177/150‑1 (MQ=60)
                                                                        AACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACT                                                                             >  SRR19973061.2540716/1‑150 (MQ=60)
                                                                        AACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACT                                                                             <  SRR19973061.2354142/150‑1 (MQ=60)
                                                                            CAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGA                                                                         >  SRR19973061.463990/1‑150 (MQ=60)
                                                                              AATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAA                                                                       >  SRR19973061.2868345/1‑150 (MQ=60)
                                                                              AATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAA                                                                       <  SRR19973061.519775/150‑1 (MQ=60)
                                                                               ATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGAACAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTTGTTGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAA                                                                      <  SRR19973061.1789332/150‑1 (MQ=60)
                                                                                TCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAA                                                                     >  SRR19973061.2346719/1‑150 (MQ=60)
                                                                                    GTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCA                                                                 >  SRR19973061.1308880/1‑150 (MQ=60)
                                                                                    GTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCA                                                                 <  SRR19973061.3177150/150‑1 (MQ=60)
                                                                                     TCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCAT                                                                <  SRR19973061.1705166/150‑1 (MQ=60)
                                                                                        CCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTT                                                             <  SRR19973061.1923321/150‑1 (MQ=60)
                                                                                         CGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTG                                                            <  SRR19973061.809309/150‑1 (MQ=60)
                                                                                         CGCGATATTCTGGTTGATGACGAAACGTTGTGTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTG                                                            <  SRR19973061.810972/150‑1 (MQ=60)
                                                                                         CGCGATATCCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTG                                                            >  SRR19973061.2523720/1‑150 (MQ=60)
                                                                                          GCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGA                                                           <  SRR19973061.3338328/150‑1 (MQ=60)
                                                                                           CGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGAT                                                          <  SRR19973061.1493935/150‑1 (MQ=60)
                                                                                           CGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGAT                                                          <  SRR19973061.1493863/150‑1 (MQ=60)
                                                                                            GATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATC                                                         >  SRR19973061.856154/1‑150 (MQ=60)
                                                                                                   TGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGG                                                  >  SRR19973061.2781056/1‑150 (MQ=60)
                                                                                                    GGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGG                                                 <  SRR19973061.735714/150‑1 (MQ=60)
                                                                                                    GGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGG                                                 <  SRR19973061.1322731/150‑1 (MQ=60)
                                                                                                      TTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCG                                               <  SRR19973061.3568108/150‑1 (MQ=60)
                                                                                                        GATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCA                                             <  SRR19973061.2907866/150‑1 (MQ=60)
                                                                                                          TGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGA                                           <  SRR19973061.675401/150‑1 (MQ=60)
                                                                                                          TGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGA                                           <  SRR19973061.675678/150‑1 (MQ=60)
                                                                                                              GAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAA                                       >  SRR19973061.1819005/1‑150 (MQ=60)
                                                                                                                  CGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCA                                   <  SRR19973061.3303988/150‑1 (MQ=60)
                                                                                                                   GTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCTCTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAG                                  <  SRR19973061.2356162/150‑1 (MQ=60)
                                                                                                                   GTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCTAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAG                                  <  SRR19973061.3222440/150‑1 (MQ=60)
                                                                                                                   GTTGTTTGAGTTCTACGAACAGCGCATCAGCCACTATGTAATCTCAGCTCGCCACTTAGACAGCTGGTTGAAAAAATTCAGCCGCGAAACGCATGATTTGCTCAACTTTGAAAAAATAATGTTGATCAAAGAGGGCGCAGAAAAAATCAG                                  <  SRR19973061.3222888/150‑1 (MQ=60)
                                                                                                                    TTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGC                                 <  SRR19973061.1507204/150‑1 (MQ=60)
                                                                                                                      GTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAA                               <  SRR19973061.1880957/150‑1 (MQ=60)
                                                                                                                         TGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCT                            >  SRR19973061.987267/1‑150 (MQ=60)
                                                                                                                         TGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCT                            <  SRR19973061.488585/150‑1 (MQ=60)
                                                                                                                         TGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCT                            <  SRR19973061.1595021/150‑1 (MQ=60)
                                                                                                                         TGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCT                            <  SRR19973061.2539958/150‑1 (MQ=60)
                                                                                                                         TGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCT                            >  SRR19973061.2895570/1‑150 (MQ=60)
                                                                                                                          GAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTG                           >  SRR19973061.2394546/1‑150 (MQ=60)
                                                                                                                          GAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTG                           <  SRR19973061.246667/150‑1 (MQ=60)
                                                                                                                                  CGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCC                   >  SRR19973061.1783513/1‑150 (MQ=60)
                                                                                                                                  CGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCC                   >  SRR19973061.1783830/1‑150 (MQ=60)
                                                                                                                                  CGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCC                   >  SRR19973061.1490205/1‑150 (MQ=60)
                                                                                                                                     CCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAA                >  SRR19973061.2756425/1‑150 (MQ=60)
                                                                                                                                      CAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAAC               <  SRR19973061.3262129/150‑1 (MQ=60)
                                                                                                                                      CAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAAC               >  SRR19973061.3511311/1‑150 (MQ=60)
                                                                                                                                           CATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTG          >  SRR19973061.1798585/1‑150 (MQ=60)
                                                                                                                                           CATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTG          >  SRR19973061.2212154/1‑150 (MQ=60)
                                                                                                                                            ATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGG         >  SRR19973061.605056/1‑150 (MQ=60)
                                                                                                                                            ATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGG         >  SRR19973061.2514150/1‑150 (MQ=60)
                                                                                                                                              CAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCA       >  SRR19973061.19014/1‑150 (MQ=60)
                                                                                                                                              CAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCA       >  SRR19973061.3495885/1‑150 (MQ=60)
                                                                                                                                               AGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCAT      <  SRR19973061.314620/150‑1 (MQ=60)
                                                                                                                                                  CACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAG   >  SRR19973061.3041911/1‑150 (MQ=60)
                                                                                                                                                   ACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGG  <  SRR19973061.2162535/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
AAGGTGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACGATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGG  >  CP009273/1479584‑1479880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑