Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,482,005 G→T 100% D172Y (GAT→TAT)  ydcF → DUF218 superfamily protein, SAM‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,482,0050GT99.5% 686.3 / ‑4.1 209D172Y (GAT→TAT) ydcFDUF218 superfamily protein, SAM‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (96/112);  total (96/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 209 total aligned reads displayed.

ACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCGATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTAC  >  CP009273/1481861‑1482149
                                                                                                                                                |                                                                                                                                                
acaaACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATg                                                                                                                                               >  1:2273219/1‑148 (MQ=255)
 caaACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGc                                                                                                                                              >  1:2867017/1‑148 (MQ=255)
   aaCTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGcac                                                                                                                                            >  2:782783/1‑148 (MQ=255)
       gCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACg                                                                                                                                        <  1:995203/148‑1 (MQ=255)
          gTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTg                                                                                                                                     >  1:3038494/1‑148 (MQ=255)
            gAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGt                                                                                                                                   >  2:2636510/1‑148 (MQ=255)
            gAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGt                                                                                                                                   >  2:1403759/1‑148 (MQ=255)
              aaaCGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTa                                                                                                                                 >  2:427027/1‑148 (MQ=255)
              aaaCGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTa                                                                                                                                 >  2:2194322/1‑148 (MQ=255)
               aaCGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTaa                                                                                                                                >  2:2409547/1‑148 (MQ=255)
               aaCGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTaa                                                                                                                                >  2:3002661/1‑148 (MQ=255)
               aaCGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTaa                                                                                                                                >  2:2738111/1‑148 (MQ=255)
                acgcacgcTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAg                                                                                                                               <  1:743808/148‑1 (MQ=255)
                   cacgcTTTATCATCGCGCTATTGAATCAGTCCGTAGAACGAGTTCATACGGCTATCTGTGTTCAGGACCACACAATGCAGCGGCGCACGAAGGCGACGTTCCGCCGTATGACTTGGGACAATCAATATTCACCACGCTGGTTAAGTTa                                                                                                                            >  2:1099464/1‑148 (MQ=255)
                      gcTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATcc                                                                                                                         <  2:3154737/148‑1 (MQ=255)
                         ttAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCgg                                                                                                                      >  1:2368649/1‑148 (MQ=255)
                          tAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGa                                                                                                                     >  2:2117859/1‑148 (MQ=255)
                            gCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGAtt                                                                                                                   <  1:1660470/148‑1 (MQ=255)
                               tCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGt                                                                                                                >  2:1496702/1‑148 (MQ=255)
                                cgcgCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGtt                                                                                                               <  2:1284985/148‑1 (MQ=255)
                                 gcgcTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTc                                                                                                              <  2:1635880/148‑1 (MQ=255)
                                     tATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCa                                                                                                          <  2:2003048/148‑1 (MQ=255)
                                        tGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGtt                                                                                                       <  1:910974/148‑1 (MQ=255)
                                        tGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGGTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGtt                                                                                                       >  1:1999401/1‑148 (MQ=255)
                                            tCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAGTCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGa                                                                                                   >  2:2120560/1‑148 (MQ=255)
                                             cAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGATGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGaa                                                                                                  <  1:895615/148‑1 (MQ=255)
                                              aGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGaaa                                                                                                 <  1:2204675/148‑1 (MQ=255)
                                               ggCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAAt                                                                                                <  2:2943809/148‑1 (MQ=255)
                                                gCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATa                                                                                               <  1:1840936/148‑1 (MQ=255)
                                                gCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATa                                                                                               <  2:1956385/148‑1 (MQ=255)
                                                gCCGTAGAACGAGTTCATACGGCTATCGTAGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATa                                                                                               >  2:847827/1‑148 (MQ=255)
                                                   gTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCNTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACg                                                                                            >  1:1785461/1‑148 (MQ=255)
                                                    tAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGc                                                                                           <  2:3311958/148‑1 (MQ=255)
                                                     aGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCa                                                                                          <  1:61351/148‑1 (MQ=255)
                                                      gAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAg                                                                                         <  2:2192611/148‑1 (MQ=255)
                                                       aaCGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGa                                                                                        >  2:530378/1‑148 (MQ=255)
                                                        aCGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGAc                                                                                       >  1:2148369/1‑148 (MQ=255)
                                                         cGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACa                                                                                      >  2:1885895/1‑148 (MQ=255)
                                                          gAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAg                                                                                     <  1:299261/148‑1 (MQ=255)
                                                           aGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAgt                                                                                    <  2:1946744/148‑1 (MQ=255)
                                                           aGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAgt                                                                                    >  2:1688854/1‑148 (MQ=255)
                                                              tCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTa                                                                                 <  1:3474693/148‑1 (MQ=255)
                                                              tCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTa                                                                                 <  1:3086953/148‑1 (MQ=255)
                                                                  aCGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCt                                                                             >  1:1188573/1‑148 (MQ=255)
                                                                  aCGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCt                                                                             <  2:1700165/148‑1 (MQ=255)
                                                                  aCGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCt                                                                             >  1:1616391/1‑148 (MQ=255)
                                                                    ggCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCttt                                                                           <  1:2664609/148‑1 (MQ=255)
                                                                       tATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTttatt                                                                        >  2:1846495/1‑148 (MQ=255)
                                                                         tCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTaa                                                                      <  2:390387/148‑1 (MQ=255)
                                                                            ttgttCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCa                                                                   >  1:1053690/1‑148 (MQ=255)
                                                                             tgttCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAg                                                                  <  2:2132272/148‑1 (MQ=255)
                                                                              gttCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGt                                                                 <  2:1976089/148‑1 (MQ=255)
                                                                              gttCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGt                                                                 <  1:3253800/148‑1 (MQ=255)
                                                                               ttCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGtt                                                                <  2:1345868/148‑1 (MQ=255)
                                                                               ttCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGtt                                                                <  1:3180289/148‑1 (MQ=255)
                                                                                tctggACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTa                                                               <  2:1338653/145‑1 (MQ=255)
                                                                                tCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTa                                                               <  2:3085428/148‑1 (MQ=255)
                                                                                tCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTa                                                               >  1:2238297/1‑148 (MQ=255)
                                                                                tCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTa                                                               >  1:3283874/1‑148 (MQ=255)
                                                                                  aGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACa                                                             <  1:2441567/148‑1 (MQ=255)
                                                                                  aGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACa                                                             <  2:2371833/148‑1 (MQ=255)
                                                                                   ggACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACaa                                                            <  2:2258789/148‑1 (MQ=255)
                                                                                     aCCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAgg                                                          >  2:610213/1‑148 (MQ=255)
                                                                                      ccccACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGa                                                         <  1:458320/148‑1 (MQ=255)
                                                                                        ccaccaTGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGAtt                                                       <  1:3298865/148‑1 (MQ=255)
                                                                                          accaTGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTAt                                                     >  2:1465959/1‑148 (MQ=255)
                                                                                          accaTGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTAt                                                     <  2:2427540/148‑1 (MQ=255)
                                                                                            caTGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGAAGACAGTGTAATATTTATTAATCAGTTACAAGGAGTATgg                                                   >  1:969717/1‑148 (MQ=255)
                                                                                             aTGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGc                                                  >  2:2587298/1‑148 (MQ=255)
                                                                                             aTGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCGATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGc                                                  <  1:356753/148‑1 (MQ=255)
                                                                                               gCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCa                                                >  2:2672681/1‑148 (MQ=255)
                                                                                               gCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGGTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCa                                                <  2:3388123/148‑1 (MQ=255)
                                                                                                 aGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGCCAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGt                                              <  2:1925545/148‑1 (MQ=255)
                                                                                                  gcggcgCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGtt                                             <  2:557047/148‑1 (MQ=255)
                                                                                                     gcgcACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAg                                          <  1:2869273/148‑1 (MQ=255)
                                                                                                       gcACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCg                                        <  1:1367285/148‑1 (MQ=255)
                                                                                                       gcACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCg                                        <  1:797294/148‑1 (MQ=255)
                                                                                                        cACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGt                                       >  2:2769515/1‑148 (MQ=255)
                                                                                                         aCGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGtt                                      <  1:3385782/148‑1 (MQ=255)
                                                                                                          cGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTa                                     <  1:575600/148‑1 (MQ=255)
                                                                                                          cGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTa                                     <  1:2117859/148‑1 (MQ=255)
                                                                                                          cGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTa                                     <  1:2741800/148‑1 (MQ=255)
                                                                                                          cGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTa                                     <  1:1275621/148‑1 (MQ=255)
                                                                                                             tGGCGACGGTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTAtct                                  <  1:2355110/148‑1 (MQ=255)
                                                                                                               gCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTAtctct                                <  2:2788296/148‑1 (MQ=255)
                                                                                                                cGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTAtctctc                               <  1:1470118/148‑1 (MQ=255)
                                                                                                                     ttCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCactac                          >  2:3070629/1‑148 (MQ=255)
                                                                                                                        cgccgTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGTATTATGGCAAGTTGAGCGTTATCTCTCACTACTCa                       >  1:3184849/1‑148 (MQ=255)
                                                                                                                        cgccgTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCa                       <  2:2867017/148‑1 (MQ=255)
                                                                                                                          ccgTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACt                     >  2:1780585/1‑148 (MQ=255)
                                                                                                                          ccgTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACt                     >  2:2921152/1‑148 (MQ=255)
                                                                                                                           cgTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTg                    >  1:1289271/1‑148 (MQ=255)
                                                                                                                               tGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGa                >  1:1712699/1‑148 (MQ=255)
                                                                                                                               tGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGa                >  1:3058217/1‑148 (MQ=255)
                                                                                                                                gACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAg               <  2:1559369/148‑1 (MQ=255)
                                                                                                                                 aCTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGc              >  1:2095045/1‑148 (MQ=255)
                                                                                                                                 aCTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGc              <  1:1292319/148‑1 (MQ=255)
                                                                                                                                 aCTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGc              >  1:2094828/1‑148 (MQ=255)
                                                                                                                                          aaTCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATACCGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGtt     <  2:74370/148‑1 (MQ=255)
                                                                                                                                            tCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTa   >  2:922862/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCGATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTAC  >  CP009273/1481861‑1482149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTCAACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCGATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTACGCGAT  >  CP009273/1481856‑1482154
                                                                                                                                                     |                                                                                                                                                     
AGTCAACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCT                                                                                                                                                       <  SRR19973065.3186560/150‑1 (MQ=60)
AGTCAACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCT                                                                                                                                                       >  SRR19973065.3408843/1‑150 (MQ=60)
   CAACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATG                                                                                                                                                    >  SRR19973065.2287570/1‑150 (MQ=60)
   CAACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATG                                                                                                                                                    >  SRR19973065.75135/1‑150 (MQ=60)
    AACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGC                                                                                                                                                   >  SRR19973065.2884608/1‑150 (MQ=60)
    AACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTGCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGC                                                                                                                                                   >  SRR19973065.2884922/1‑150 (MQ=60)
      CAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCAC                                                                                                                                                 >  SRR19973065.1569717/1‑150 (MQ=60)
            GCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCT                                                                                                                                           <  SRR19973065.1001818/150‑1 (MQ=60)
            GCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCT                                                                                                                                           <  SRR19973065.1001999/150‑1 (MQ=60)
            GCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCT                                                                                                                                           <  SRR19973065.1001814/150‑1 (MQ=60)
             CGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTG                                                                                                                                          >  SRR19973065.3057100/1‑150 (MQ=60)
                  AAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAA                                                                                                                                     >  SRR19973065.1286971/1‑150 (MQ=60)
                     ACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTT                                                                                                                                  <  SRR19973065.748790/150‑1 (MQ=60)
                       GCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTAT                                                                                                                                <  SRR19973065.3057428/150‑1 (MQ=60)
                       GCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTAT                                                                                                                                >  SRR19973065.402143/1‑150 (MQ=60)
                         ACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCC                                                                                                                              >  SRR19973065.868994/1‑150 (MQ=60)
                            CTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGG                                                                                                                           >  SRR19973065.2383418/1‑150 (MQ=60)
                            CTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGG                                                                                                                           >  SRR19973065.2383406/1‑150 (MQ=60)
                                 GCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCG                                                                                                                      <  SRR19973065.1671592/150‑1 (MQ=60)
                                 GCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCG                                                                                                                      <  SRR19973065.1671594/150‑1 (MQ=60)
                                           ATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTT                                                                                                            <  SRR19973065.2838378/150‑1 (MQ=60)
                                           ATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGGTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTT                                                                                                            >  SRR19973065.2012345/1‑150 (MQ=60)
                                             TGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAG                                                                                                          <  SRR19973065.916900/150‑1 (MQ=60)
                                             TGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAG                                                                                                          <  SRR19973065.914861/150‑1 (MQ=60)
                                                  CAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGATGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAAT                                                                                                     <  SRR19973065.901326/150‑1 (MQ=60)
                                                  CAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGATGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAAT                                                                                                     <  SRR19973065.901338/150‑1 (MQ=60)
                                                   AGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATA                                                                                                    <  SRR19973065.2218781/150‑1 (MQ=60)
                                                   AGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATA                                                                                                    >  SRR19973065.3095194/1‑150 (MQ=60)
                                                   AGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATA                                                                                                    <  SRR19973065.2442576/150‑1 (MQ=60)
                                                   AGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATA                                                                                                    >  SRR19973065.1809427/1‑150 (MQ=60)
                                                     GCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAAC                                                                                                  <  SRR19973065.1853081/150‑1 (MQ=60)
                                                      CCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCNTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACG                                                                                                 >  SRR19973065.1797146/1‑150 (MQ=60)
                                                      CCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACG                                                                                                 >  SRR19973065.2214673/1‑150 (MQ=60)
                                                        GTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCA                                                                                               <  SRR19973065.1264581/150‑1 (MQ=60)
                                                          AGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGA                                                                                             <  SRR19973065.62003/150‑1 (MQ=60)
                                                          AGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGA                                                                                             <  SRR19973065.1445892/150‑1 (MQ=60)
                                                           GAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGAC                                                                                            <  SRR19973065.1950363/150‑1 (MQ=60)
                                                           GAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGAC                                                                                            <  SRR19973065.3049658/150‑1 (MQ=60)
                                                           GAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGAC                                                                                            >  SRR19973065.2162183/1‑150 (MQ=60)
                                                           GAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGAC                                                                                            <  SRR19973065.2347549/150‑1 (MQ=60)
                                                               GAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTG                                                                                        <  SRR19973065.301200/150‑1 (MQ=60)
                                                                   TCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAAT                                                                                    <  SRR19973065.3495832/150‑1 (MQ=60)
                                                                   TCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAAT                                                                                    <  SRR19973065.3105805/150‑1 (MQ=60)
                                                                   TCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAAT                                                                                    <  SRR19973065.3106216/150‑1 (MQ=60)
                                                                   TCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAAT                                                                                    <  SRR19973065.3495890/150‑1 (MQ=60)
                                                                     ATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCT                                                                                  >  SRR19973065.3357757/1‑150 (MQ=60)
                                                                     ATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCT                                                                                  >  SRR19973065.1196088/1‑150 (MQ=60)
                                                                     ATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCT                                                                                  >  SRR19973065.1627238/1‑150 (MQ=60)
                                                                         GGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTAT                                                                              <  SRR19973065.2681097/150‑1 (MQ=60)
                                                                             ATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAAT                                                                          <  SRR19973065.3265990/150‑1 (MQ=60)
                                                                              TCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATC                                                                         <  SRR19973065.3476889/150‑1 (MQ=60)
                                                                              TCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATC                                                                         <  SRR19973065.2516161/150‑1 (MQ=60)
                                                                              TCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATC                                                                         <  SRR19973065.2267820/150‑1 (MQ=60)
                                                                               CGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCA                                                                        >  SRR19973065.1060677/1‑150 (MQ=60)
                                                                                GTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAG                                                                       <  SRR19973065.3300885/150‑1 (MQ=60)
                                                                                   GTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTA                                                                    >  SRR19973065.2252528/1‑150 (MQ=60)
                                                                                   GTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTA                                                                    >  SRR19973065.3303840/1‑150 (MQ=60)
                                                                                   GTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTA                                                                    <  SRR19973065.3273473/150‑1 (MQ=60)
                                                                                   GTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTA                                                                    >  SRR19973065.2252136/1‑150 (MQ=60)
                                                                                    TTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTAC                                                                   <  SRR19973065.3199509/150‑1 (MQ=60)
                                                                                    TTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTAC                                                                   <  SRR19973065.3200377/150‑1 (MQ=60)
                                                                                       AGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAG                                                                <  SRR19973065.2456954/150‑1 (MQ=60)
                                                                                       AGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAG                                                                <  SRR19973065.3309294/150‑1 (MQ=60)
                                                                                           CCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATT                                                            <  SRR19973065.461481/150‑1 (MQ=60)
                                                                                             CCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTAT                                                          <  SRR19973065.3318956/150‑1 (MQ=60)
                                                                                               ACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGG                                                        >  SRR19973065.975807/1‑150 (MQ=60)
                                                                                               ACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGAAGACAGTGTAATATTTATTAATCAGTTACAAGGAGTATGG                                                        >  SRR19973065.976154/1‑150 (MQ=60)
                                                                                                  ATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCGATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCA                                                     <  SRR19973065.359266/150‑1 (MQ=60)
                                                                                                     CAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTT                                                  >  SRR19973065.579416/1‑150 (MQ=60)
                                                                                                       GCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGA                                                <  SRR19973065.2554858/150‑1 (MQ=60)
                                                                                                          GCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCG                                             <  SRR19973065.2886870/150‑1 (MQ=60)
                                                                                                            GCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTT                                           <  SRR19973065.1376320/150‑1 (MQ=60)
                                                                                                            GCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTT                                           <  SRR19973065.802564/150‑1 (MQ=60)
                                                                                                              ACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTAT                                         >  SRR19973065.2115235/1‑150 (MQ=60)
                                                                                                              ACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTAT                                         <  SRR19973065.3406487/150‑1 (MQ=60)
                                                                                                               CGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATC                                        <  SRR19973065.579214/150‑1 (MQ=60)
                                                                                                               CGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATC                                        <  SRR19973065.1284036/150‑1 (MQ=60)
                                                                                                               CGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATC                                        <  SRR19973065.2758852/150‑1 (MQ=60)
                                                                                                               CGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATC                                        <  SRR19973065.2755145/150‑1 (MQ=60)
                                                                                                               CGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATC                                        <  SRR19973065.2131502/150‑1 (MQ=60)
                                                                                                               CGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATC                                        <  SRR19973065.2131488/150‑1 (MQ=60)
                                                                                                                  TGGCGACGGTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCT                                     <  SRR19973065.2369754/150‑1 (MQ=60)
                                                                                                                   GGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTC                                    <  SRR19973065.516397/150‑1 (MQ=60)
                                                                                                                     CGACTGTCCGCCGTATGACTGGGGACAATCCATATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCAC                                  <  SRR19973065.1479578/150‑1 (MQ=60)
                                                                                                                     CGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCAC                                  <  SRR19973065.1479541/150‑1 (MQ=60)
                                                                                                                         GTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACT                              <  SRR19973065.787980/150‑1 (MQ=60)
                                                                                                                           TCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGTATTATGGCAAGTTGAGCGTTATCTCTCACTACTCA                            >  SRR19973065.3204080/1‑150 (MQ=60)
                                                                                                                             CGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACT                          <  SRR19973065.2942195/150‑1 (MQ=60)
                                                                                                                              GCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTG                         >  SRR19973065.1297892/1‑150 (MQ=60)
                                                                                                                              GCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTG                         >  SRR19973065.1297772/1‑150 (MQ=60)
                                                                                                                                  TATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGA                     >  SRR19973065.1725794/1‑150 (MQ=60)
                                                                                                                                  TATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGA                     >  SRR19973065.1724036/1‑150 (MQ=60)
                                                                                                                                  TATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGA                     >  SRR19973065.1724092/1‑150 (MQ=60)
                                                                                                                                  TATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGA                     >  SRR19973065.3076910/1‑150 (MQ=60)
                                                                                                                                    TGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGC                   >  SRR19973065.2108279/1‑150 (MQ=60)
                                                                                                                                    TGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGC                   >  SRR19973065.2108290/1‑150 (MQ=60)
                                                                                                                                    TGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGC                   >  SRR19973065.2108503/1‑150 (MQ=60)
                                                                                                                                    TGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGC                   >  SRR19973065.2108510/1‑150 (MQ=60)
                                                                                                                                      ACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTG                 <  SRR19973065.1301144/150‑1 (MQ=60)
                                                                                                                                      ACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTG                 <  SRR19973065.1300833/150‑1 (MQ=60)
                                                                                                                                            GACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGT           >  SRR19973065.3282662/1‑150 (MQ=60)
                                                                                                                                             ACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTT          <  SRR19973065.189151/150‑1 (MQ=60)
                                                                                                                                                ATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTAC       >  SRR19973065.1134517/1‑150 (MQ=60)
                                                                                                                                                  CCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTACGC     >  SRR19973065.1472434/1‑150 (MQ=60)
                                                                                                                                                    CTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTACGCGA   >  SRR19973065.2926201/1‑150 (MQ=60)
                                                                                                                                                     TATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTACGCGAT  <  SRR19973065.164291/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AGTCAACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCGATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTACGCGAT  >  CP009273/1481856‑1482154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑