Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,261,810 G→A 83.5% A87A (GCG→GCA)  prmC → N5‑glutamine methyltransferase, modifies release factors RF‑1 and RF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,261,8100GA83.5% 749.2 / 135.8 297A87A (GCG→GCA) prmCN5‑glutamine methyltransferase, modifies release factors RF‑1 and RF‑2
Reads supporting (aligned to +/- strand):  ref base G (26/23);  new base A (118/129);  total (145/152)
Fisher's exact test for biased strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 298 total aligned reads displayed.

TATTCTCGCCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTA  >  CP009273/1261663‑1261958
                                                                                                                                                   |                                                                                                                                                    
  ttCTCGCCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAAcc                                                                                                                                                   >  2:4243358/1‑149 (MQ=255)
   tctcGCCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCt                                                                                                                                                  <  2:2365582/149‑1 (MQ=255)
   tctcGCCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCt                                                                                                                                                  <  2:2260595/149‑1 (MQ=255)
   tctcGCCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCt                                                                                                                                                  <  2:4055856/149‑1 (MQ=255)
   tctcGCCTTTGGAGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCt                                                                                                                                                  <  2:1668643/149‑1 (MQ=255)
    ctcGCCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCtt                                                                                                                                                 <  2:3468272/149‑1 (MQ=255)
     tcGCCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTa                                                                                                                                                <  2:51317/149‑1 (MQ=255)
     tcGCCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTa                                                                                                                                                >  2:1076156/1‑149 (MQ=255)
      cGCCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTaa                                                                                                                                               >  1:4382363/1‑149 (MQ=255)
         cTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTc                                                                                                                                            >  1:2508579/1‑149 (MQ=255)
             ggTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCcgcg                                                                                                                                        >  2:1368084/1‑149 (MQ=255)
               tGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGcc                                                                                                                                      <  2:293706/149‑1 (MQ=255)
                   aCGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGa                                                                                                                                  <  1:1983411/149‑1 (MQ=255)
                   aCGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGa                                                                                                                                  >  1:589631/1‑149 (MQ=255)
                   aCGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGa                                                                                                                                  <  1:4259332/149‑1 (MQ=255)
                    cGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGAt                                                                                                                                 <  2:194564/149‑1 (MQ=255)
                    cGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGAt                                                                                                                                 >  2:1057107/1‑149 (MQ=255)
                    cGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGAt                                                                                                                                 <  2:4728703/149‑1 (MQ=255)
                     gCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATa                                                                                                                                >  1:1180686/1‑149 (MQ=255)
                       aGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACg                                                                                                                              <  1:1211220/149‑1 (MQ=255)
                        gCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACgg                                                                                                                             >  2:2171418/1‑149 (MQ=255)
                         ctgactgaCGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTGGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGa                                                                                                                            >  1:4759874/1‑149 (MQ=255)
                           gactgacGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAgt                                                                                                                          <  2:1450246/149‑1 (MQ=255)
                              tgacGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTTTTTCGCCAGCAAACATAATTACGCGCCCGGATACGGAGTGTc                                                                                                                       >  1:3670539/1‑149 (MQ=255)
                              tgacGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTc                                                                                                                       >  1:4313881/1‑149 (MQ=255)
                                  gAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCtggt                                                                                                                   >  1:84382/1‑149 (MQ=255)
                                   aaCAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCtggtg                                                                                                                  <  2:571899/149‑1 (MQ=255)
                                   aaCAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCtgggg                                                                                                                  >  2:4153363/1‑147 (MQ=255)
                                     cAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAAGTCCGCGCCCGGATACGGAGTGTCTGGTGGa                                                                                                                <  2:4361612/149‑1 (MQ=255)
                                     cAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGa                                                                                                                <  1:1285882/149‑1 (MQ=255)
                                          tCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCATCAACCATAATTACGCGCCCGGATACGGAGTGTCTTGTGGAGCAgg                                                                                                           >  1:1439608/1‑149 (MQ=255)
                                            aGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCa                                                                                                         <  2:3965039/149‑1 (MQ=255)
                                               aaCTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTg                                                                                                      >  2:4217921/1‑149 (MQ=255)
                                               aaCTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTg                                                                                                      <  1:2144319/149‑1 (MQ=255)
                                                aCTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTgg                                                                                                     <  1:3360722/149‑1 (MQ=255)
                                                 cTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGgc                                                                                                    <  1:2772474/149‑1 (MQ=255)
                                                      tGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGt                                                                                               <  1:185749/149‑1 (MQ=255)
                                                         gcTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGc                                                                                            <  2:339076/149‑1 (MQ=255)
                                                          cTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGcc                                                                                           >  1:634566/1‑149 (MQ=255)
                                                          cTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGcc                                                                                           <  1:769028/149‑1 (MQ=255)
                                                          cTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGcc                                                                                           <  2:389288/149‑1 (MQ=255)
                                                              tcacacGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAAATCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGaa                                                                                       >  2:3241955/3‑149 (MQ=255)
                                                              tGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGaa                                                                                       <  1:3030960/149‑1 (MQ=255)
                                                              tGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGaa                                                                                       <  1:3796414/149‑1 (MQ=255)
                                                               gACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGaac                                                                                      >  2:2068307/1‑149 (MQ=255)
                                                                  acGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGaacaac                                                                                   >  2:3704906/1‑149 (MQ=255)
                                                                   cgtcgtCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAAcc                                                                                  <  2:3202419/149‑1 (MQ=255)
                                                                   cgtcgtCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGTAGGCACTGGCGCGGTTGCCTGAACAAcc                                                                                  <  1:4494193/149‑1 (MQ=255)
                                                                     tcgtcgCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCtt                                                                                <  1:697991/149‑1 (MQ=255)
                                                                     gcgtcgCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCGGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCtt                                                                                >  2:951954/2‑149 (MQ=255)
                                                                          gcgATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGt                                                                           <  1:4505610/149‑1 (MQ=255)
                                                                          gcgATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATATTTTTATCCAGCAACCTTAATTACCAGCCCGGGTACGGAGTGTATGGTGGAGCAGGCACTGGCGCGGGTGACTGAACAACCTTGCCGt                                                                           >  1:4222373/1‑149 (MQ=255)
                                                                           cgATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCATGCCGTa                                                                          >  1:264964/1‑149 (MQ=255)
                                                                              tGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATtc                                                                       <  2:1531053/149‑1 (MQ=255)
                                                                              tGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATtc                                                                       <  1:3516539/149‑1 (MQ=255)
                                                                                   aaCCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGAt                                                                  >  2:1895329/1‑149 (MQ=255)
                                                                                   aaCCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGAt                                                                  <  2:1984781/149‑1 (MQ=255)
                                                                                    aCCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTTTTTATCCAGCAAACATAATTAAGCGCCCGGATACGGAGTGTCTGGTGGTGCAGGCCCTTGCGCGGTTGCCTGAACAACCTTGCCGTATTCACGAtc                                                                 >  1:3297343/1‑149 (MQ=255)
                                                                                        aTTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCgg                                                             >  2:579263/1‑149 (MQ=255)
                                                                                          tGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGa                                                           <  2:4313406/149‑1 (MQ=255)
                                                                                                tttCACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTGTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTa                                                     <  2:4077788/149‑1 (MQ=255)
                                                                                                tttAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTAGCCAGCAACCATAATTCCGCGCCCGGATACGGAGTGTCGGGTGGAGCAGGCACTGGCGCCGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTa                                                     >  1:4364510/1‑149 (MQ=255)
                                                                                                  tAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTAcc                                                   <  2:1006176/149‑1 (MQ=255)
                                                                                                  tAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTAcc                                                   <  2:486371/149‑1 (MQ=255)
                                                                                                   aaCCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCg                                                  >  2:2481581/1‑149 (MQ=255)
                                                                                                   aaCCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCg                                                  >  2:89277/1‑149 (MQ=255)
                                                                                                    aCCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCgg                                                 >  1:1516657/1‑149 (MQ=255)
                                                                                                        gggTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCg                                             >  2:2104222/1‑149 (MQ=255)
                                                                                                        gggTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCg                                             >  2:4790598/1‑149 (MQ=255)
                                                                                                        gggTGCGAGAAGTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCg                                             <  2:2663692/149‑1 (MQ=255)
                                                                                                          gTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGAt                                           <  2:3056776/149‑1 (MQ=255)
                                                                                                          gTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGAt                                           >  2:1820828/1‑149 (MQ=255)
                                                                                                              gagaATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGAttgcg                                       >  2:2881133/1‑149 (MQ=255)
                                                                                                               agaATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGAttgcgc                                      >  2:1966689/1‑149 (MQ=255)
                                                                                                                gaATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCATAAATACGCGCCCGGAAACGGAGTGTCTGGTGGAGCAGGCACATGCGCCGTTGCCTGAACAACCATTCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGAtttcgct                                     >  1:1040348/1‑149 (MQ=255)
                                                                                                                 aaTTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGAttgcgctt                                    <  1:2565127/149‑1 (MQ=255)
                                                                                                                     cTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCATAATTACGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCACGATCTCGGGACGGGTACCGGGGCGAttgcgcttgcgc                                >  1:397677/1‑149 (MQ=255)
                                                                                                                       ggTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTg                              >  1:3832826/1‑149 (MQ=255)
                                                                                                                         tCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGc                            <  2:2748069/149‑1 (MQ=255)
                                                                                                                         tCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGc                            <  2:409931/149‑1 (MQ=255)
                                                                                                                          cGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCt                           <  2:3732004/149‑1 (MQ=255)
                                                                                                                           gTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTa                          <  2:4456415/149‑1 (MQ=255)
                                                                                                                            ttGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTag                         >  2:929891/1‑149 (MQ=255)
                                                                                                                             tGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTagc                        >  1:4567141/1‑149 (MQ=255)
                                                                                                                             tGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTagc                        >  1:3386001/1‑149 (MQ=255)
                                                                                                                              gCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTagcg                       >  1:2287166/1‑149 (MQ=255)
                                                                                                                              gCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTagcg                       >  1:1589651/1‑149 (MQ=255)
                                                                                                                              gCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTagcg                       <  2:4575220/149‑1 (MQ=255)
                                                                                                                              gCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTagcg                       >  1:507752/1‑149 (MQ=255)
                                                                                                                                 gTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTagcgagc                    >  1:2345100/1‑149 (MQ=255)
                                                                                                                                 gTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTagcgagc                    >  1:171123/1‑149 (MQ=255)
                                                                                                                                 gTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTagcgagc                    <  1:1007444/149‑1 (MQ=255)
                                                                                                                                    aTTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGcc                 >  2:66309/1‑149 (MQ=255)
                                                                                                                                     tttGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGccc                <  1:3573997/149‑1 (MQ=255)
                                                                                                                                     tttGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGccc                <  1:3172275/149‑1 (MQ=255)
                                                                                                                                      ttGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCg               <  1:461349/149‑1 (MQ=255)
                                                                                                                                        gTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGa             <  1:1034516/149‑1 (MQ=255)
                                                                                                                                            cGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGc         <  2:1217422/149‑1 (MQ=255)
                                                                                                                                              ccAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGa       >  1:2384682/1‑149 (MQ=255)
                                                                                                                                                aGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGaaa     >  1:3178679/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TATTCTCGCCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTA  >  CP009273/1261663‑1261958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTATTCTCGCCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTAT  >  CP009273/1261662‑1261959
                                                                                                                                                    |                                                                                                                                                     
TTATTCTCGCCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGA                                                                                                                                                      >  SRR19973071.3993267/1‑150 (MQ=60)
      TCGCCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAA                                                                                                                                                >  SRR19973071.4397717/1‑150 (MQ=60)
      TCGCCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAA                                                                                                                                                >  SRR19973071.4397751/1‑150 (MQ=60)
      TCGCCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAA                                                                                                                                                <  SRR19973071.3474709/150‑1 (MQ=60)
         CCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTC                                                                                                                                             >  SRR19973071.2517712/1‑150 (MQ=60)
           TTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCG                                                                                                                                           <  SRR19973071.3816379/150‑1 (MQ=60)
             TGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCG                                                                                                                                         >  SRR19973071.1036016/1‑150 (MQ=60)
              GGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGC                                                                                                                                        >  SRR19973071.1897410/1‑150 (MQ=60)
                TGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCC                                                                                                                                      >  SRR19973071.609976/1‑150 (MQ=60)
                  AAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGG                                                                                                                                    >  SRR19973071.252825/1‑150 (MQ=60)
                   AACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGA                                                                                                                                   >  SRR19973071.591830/1‑150 (MQ=60)
                    ACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGAT                                                                                                                                  <  SRR19973071.4298073/150‑1 (MQ=60)
                    ACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGAT                                                                                                                                  <  SRR19973071.4274440/150‑1 (MQ=60)
                    ACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGAT                                                                                                                                  <  SRR19973071.1990583/150‑1 (MQ=60)
                     CGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATA                                                                                                                                 <  SRR19973071.368107/150‑1 (MQ=60)
                     CGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATA                                                                                                                                 >  SRR19973071.1184750/1‑150 (MQ=60)
                     CGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATA                                                                                                                                 >  SRR19973071.2683560/1‑150 (MQ=60)
                      GCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATAC                                                                                                                                >  SRR19973071.3304567/1‑150 (MQ=60)
                        AGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGG                                                                                                                              <  SRR19973071.1215391/150‑1 (MQ=60)
                         GCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCATAATTACGCGCCCGGATACGGA                                                                                                                             >  SRR19973071.1221642/1‑150 (MQ=60)
                         GCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTGGCCGTTATTTGTTTCGCCAGCCACCTTAATTCCGCGCCCGGATACGGA                                                                                                                             >  SRR19973071.4776697/1‑150 (MQ=60)
                              CTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTTTTTCGCCAGCAAACATAATTACGCGCCCGGATACGGAGTGTC                                                                                                                        >  SRR19973071.3683498/1‑150 (MQ=60)
                              CTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTC                                                                                                                        <  SRR19973071.790401/150‑1 (MQ=60)
                              CTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTC                                                                                                                        <  SRR19973071.790459/150‑1 (MQ=60)
                              CTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTC                                                                                                                        >  SRR19973071.4328652/1‑150 (MQ=60)
                              CTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTC                                                                                                                        >  SRR19973071.4329127/1‑150 (MQ=60)
                               TGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCT                                                                                                                       <  SRR19973071.4491267/150‑1 (MQ=60)
                               TGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCT                                                                                                                       <  SRR19973071.4491268/150‑1 (MQ=60)
                                GACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTG                                                                                                                      <  SRR19973071.2256842/150‑1 (MQ=60)
                                 ACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGG                                                                                                                     >  SRR19973071.3544225/1‑150 (MQ=60)
                                 ACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGG                                                                                                                     >  SRR19973071.3544295/1‑150 (MQ=60)
                                  CGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGT                                                                                                                    >  SRR19973071.84827/1‑150 (MQ=60)
                                  CGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGT                                                                                                                    <  SRR19973071.541981/150‑1 (MQ=60)
                                   GAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTG                                                                                                                   <  SRR19973071.3363821/150‑1 (MQ=60)
                                    AACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGG                                                                                                                  >  SRR19973071.2536667/1‑150 (MQ=60)
                                    AACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGG                                                                                                                  <  SRR19973071.3662406/150‑1 (MQ=60)
                                      CAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAG                                                                                                                <  SRR19973071.4369649/150‑1 (MQ=60)
                                      CAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAG                                                                                                                <  SRR19973071.4369672/150‑1 (MQ=60)
                                      CAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAG                                                                                                                <  SRR19973071.1290558/150‑1 (MQ=60)
                                       AATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGC                                                                                                               <  SRR19973071.2450059/150‑1 (MQ=60)
                                        ATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTAGCCAGCAAACTTAAATAAGCGCCCGGATACGGAGTGTCTTGTGGAGCA                                                                                                              >  SRR19973071.2584264/1‑150 (MQ=60)
                                          GTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCATCAACCATAATTACGCGCCCGGATACGGAGTGTCTTGTGGAGCAGG                                                                                                            >  SRR19973071.1444888/1‑150 (MQ=60)
                                            CAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGTGCCCGGATACGGAGTGTCTGGTGGAGCAGGCA                                                                                                          <  SRR19973071.852291/150‑1 (MQ=60)
                                            CAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGTGCCCGGATACGGAGTGTCTGGTGGAGCAGGCA                                                                                                          <  SRR19973071.3250448/150‑1 (MQ=60)
                                            CAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCA                                                                                                          >  SRR19973071.2258757/1‑150 (MQ=60)
                                              GCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACT                                                                                                        <  SRR19973071.2333138/150‑1 (MQ=60)
                                                AACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGG                                                                                                      <  SRR19973071.4127057/150‑1 (MQ=60)
                                                AACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGG                                                                                                      <  SRR19973071.2152234/150‑1 (MQ=60)
                                                 ACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGC                                                                                                     <  SRR19973071.2391924/150‑1 (MQ=60)
                                                 ACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGC                                                                                                     <  SRR19973071.3372690/150‑1 (MQ=60)
                                                 ACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGC                                                                                                     <  SRR19973071.3372649/150‑1 (MQ=60)
                                                 ACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGC                                                                                                     <  SRR19973071.3372647/150‑1 (MQ=60)
                                                  CTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCG                                                                                                    >  SRR19973071.4566287/1‑150 (MQ=60)
                                                  CTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCG                                                                                                    <  SRR19973071.2782235/150‑1 (MQ=60)
                                                  CTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCG                                                                                                    >  SRR19973071.4566340/1‑150 (MQ=60)
                                                       TGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTT                                                                                               <  SRR19973071.186519/150‑1 (MQ=60)
                                                       TGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTT                                                                                               <  SRR19973071.4772275/150‑1 (MQ=60)
                                                       TGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTT                                                                                               <  SRR19973071.1486609/150‑1 (MQ=60)
                                                          GCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCC                                                                                            >  SRR19973071.636895/1‑150 (MQ=60)
                                                           CTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCT                                                                                           <  SRR19973071.771629/150‑1 (MQ=60)
                                                               TGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAAC                                                                                       <  SRR19973071.3809904/150‑1 (MQ=60)
                                                               TGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAAC                                                                                       <  SRR19973071.3041747/150‑1 (MQ=60)
                                                                    CGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGTAGGCACTGGCGCGGTTGCCTGAACAACCT                                                                                  <  SRR19973071.4510168/150‑1 (MQ=60)
                                                                    CGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGTAGGCACTGGCGCGGTTGCCTGAACAACCT                                                                                  <  SRR19973071.4510211/150‑1 (MQ=60)
                                                                    CGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGTAGGCACTGGCGCGGTTGCCTGAACAACCT                                                                                  <  SRR19973071.4510329/150‑1 (MQ=60)
                                                                      TCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTG                                                                                <  SRR19973071.700454/150‑1 (MQ=60)
                                                                          CGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTTTTTAGCCAGCAAACATAATTACGCGCCCGGATACGGAGTGTGTGGTGGAGCAGGCACTGGCGCGGGTGCCTGAAAAACCATGCCGG                                                                            >  SRR19973071.1259445/1‑150 (MQ=60)
                                                                          CGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCACCAACCTTAATTACGCGCCCGGAAAAGGAGTGTCTGGTGGAGCAGGCACAGGCGCGGTTGCCAGAACAACCATGCCGT                                                                            >  SRR19973071.4237909/1‑150 (MQ=60)
                                                                          CGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATATTTTTATCCAGCAACCTTAATTACCAGCCCGGGTACGGAGTGTATGGTGGAGCAGGCACTGGCGCGGGTGACTGAACAACCTTGCCGT                                                                            >  SRR19973071.4237392/1‑150 (MQ=60)
                                                                           GCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTA                                                                           <  SRR19973071.4521624/150‑1 (MQ=60)
                                                                           GCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCATGCCGTA                                                                           >  SRR19973071.265945/1‑150 (MQ=60)
                                                                               TGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCT                                                                       <  SRR19973071.629447/150‑1 (MQ=60)
                                                                               TGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCT                                                                       <  SRR19973071.3528869/150‑1 (MQ=60)
                                                                                GGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTC                                                                      <  SRR19973071.752104/150‑1 (MQ=60)
                                                                                GGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTC                                                                      <  SRR19973071.751859/150‑1 (MQ=60)
                                                                                   GAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGAT                                                                   >  SRR19973071.1199843/1‑150 (MQ=60)
                                                                                   GAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAAACTTGCCGTATTCTCGAT                                                                   >  SRR19973071.159278/1‑150 (MQ=60)
                                                                                   GAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCATAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGAT                                                                   >  SRR19973071.396830/1‑150 (MQ=60)
                                                                                    AACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTTTTTATCCAGCAAACATAATTAAGCGCCCGGATACGGAGTGTCTGGTGGTGCAGGCCCTTGCGCGGTTGCCTGAACAACCTTGCCGTATTCACGATC                                                                  >  SRR19973071.3309031/1‑150 (MQ=60)
                                                                                    AACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTTTTTAGCCAGCAACCATAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAAAAACCATTCCGTATTCTCGATC                                                                  >  SRR19973071.488651/1‑150 (MQ=60)
                                                                                    AACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATC                                                                  <  SRR19973071.2390783/150‑1 (MQ=60)
                                                                                             CTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGG                                                         >  SRR19973071.299254/1‑150 (MQ=60)
                                                                                                ATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTA                                                      >  SRR19973071.4380084/1‑150 (MQ=60)
                                                                                                ATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTAGCCAGCAACCATAATTCCGCGCCCGGATACGGAGTGTCGGGTGGAGCAGGCACTGGCGCCGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTA                                                      >  SRR19973071.4379866/1‑150 (MQ=60)
                                                                                                   TAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCCGCCACCATAATTCCGCGCCCCGATAAGGAGTGTCTGGTGGAGCAGGCACAGGCGCGGTTGCCTGAACAACCGTGCCGTATTCTCGATCTCGGGACGGGGACCG                                                   >  SRR19973071.481798/1‑150 (MQ=60)
                                                                                                   TAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTAGCCAGCGACCGTAATTACGCGCCCGGCTACGGAGTGTGTGGTGGGGCCGGCCCAGGCGCGGTTGCCGGAACAACCTTGCCGGATTCTCGGTCTCGGGACGGGTACCG                                                   >  SRR19973071.1647889/1‑150 (MQ=60)
                                                                                                    AACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGG                                                  >  SRR19973071.1522130/1‑150 (MQ=60)
                                                                                                        GGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCG                                              >  SRR19973071.1908846/1‑150 (MQ=60)
                                                                                                            TGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCACCAAACATAATTACGCGCCCCGATACGGAGTGTGTGGTGGAGCAGGCACTGGCGCGGTTGCCGGAACAACCATGCCGTATTATCGATCTCGGGACGGGTACCGGGGCGATTT                                          >  SRR19973071.2459017/1‑150 (MQ=60)
                                                                                                              CGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGCCCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCATGCCGTATTATCGATCTCGGGACGGGTACCGGGGCGATTGCG                                        >  SRR19973071.3325255/1‑150 (MQ=60)
                                                                                                              CGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCG                                        >  SRR19973071.1516793/1‑150 (MQ=60)
                                                                                                               GAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCACCAACCATAAATACGCGCCCGGCAACCGAGTGTCTGGTGGAGCACGCCCAGGCGCGGTTGCCAGAACAACCATGCCCGAATCACGATCACGGGACCGGTACCCGGGCGATTGCGC                                       >  SRR19973071.3700049/1‑150 (MQ=38)
                                                                                                                AGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCT                                      <  SRR19973071.2346667/150‑1 (MQ=60)
                                                                                                                AGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCATAAATACGCGCCCGGAAACGGAGTGTCTGGTGGAGCAGGCACATGCGCCGTTGCCTGAACAACCATTCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTTCGCT                                      >  SRR19973071.1044096/1‑150 (MQ=60)
                                                                                                                AGAATTCTGGTCGTTGCCGTTATTTGTTTAGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACAGGCGCGGGTGCCTGAACAACCATGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCT                                      >  SRR19973071.1043823/1‑150 (MQ=60)
                                                                                                                  AATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTG                                    <  SRR19973071.2574434/150‑1 (MQ=60)
                                                                                                                   ATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCATAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCATGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGC                                   >  SRR19973071.3942215/1‑150 (MQ=60)
                                                                                                                    TTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCG                                  <  SRR19973071.4608081/150‑1 (MQ=60)
                                                                                                                     TCTGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCATAATTACGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCACGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGC                                 >  SRR19973071.398910/1‑150 (MQ=60)
                                                                                                                       TGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTG                               <  SRR19973071.1143358/150‑1 (MQ=60)
                                                                                                                       TGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTG                               >  SRR19973071.3846410/1‑150 (MQ=60)
                                                                                                                       TGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTG                               >  SRR19973071.3846367/1‑150 (MQ=60)
                                                                                                                       TGGTCGTTGCCGTTATTTGTTTCGCCAGCAACCATAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCATGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTG                               >  SRR19973071.1119756/1‑150 (MQ=60)
                                                                                                                       TGGTCGTTGCCGTTATTTGTTTAGCCAGCAACCATAATTACGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACAGGCGCGGTTGCCTGAACAAACATGCCGTATTCTCGATATCGGGACGGGTACCGGGGCGATTGCGCTTtcgcat                               >  SRR19973071.1737717/1‑144 (MQ=60)
                                                                                                                         GTCGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGC                             >  SRR19973071.1527400/1‑150 (MQ=60)
                                                                                                                           CGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTA                           <  SRR19973071.2096716/150‑1 (MQ=60)
                                                                                                                           CGTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTA                           <  SRR19973071.3017937/150‑1 (MQ=60)
                                                                                                                            GTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAG                          <  SRR19973071.3755727/150‑1 (MQ=60)
                                                                                                                            GTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAG                          <  SRR19973071.533506/150‑1 (MQ=60)
                                                                                                                            GTTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACAGGCGCGGTTTCCTGAACAACCTTGCCGTATTATCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAG                          >  SRR19973071.902081/1‑150 (MQ=60)
                                                                                                                             TTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGC                         >  SRR19973071.2757796/1‑150 (MQ=60)
                                                                                                                             TTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGC                         >  SRR19973071.3398021/1‑150 (MQ=60)
                                                                                                                             TTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGC                         >  SRR19973071.3398012/1‑150 (MQ=60)
                                                                                                                             TTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGC                         >  SRR19973071.4583431/1‑150 (MQ=60)
                                                                                                                             TTGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGC                         >  SRR19973071.4583412/1‑150 (MQ=60)
                                                                                                                              TGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCG                        >  SRR19973071.1595425/1‑150 (MQ=60)
                                                                                                                              TGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCG                        >  SRR19973071.1595267/1‑150 (MQ=60)
                                                                                                                              TGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCG                        >  SRR19973071.509640/1‑150 (MQ=60)
                                                                                                                              TGCCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCG                        >  SRR19973071.2295553/1‑150 (MQ=60)
                                                                                                                                CCGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAG                      >  SRR19973071.1281974/1‑150 (MQ=60)
                                                                                                                                 CGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGC                     >  SRR19973071.171850/1‑150 (MQ=60)
                                                                                                                                 CGTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGC                     >  SRR19973071.2353614/1‑150 (MQ=60)
                                                                                                                                  GTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGATCG                    >  SRR19973071.2588379/1‑150 (MQ=60)
                                                                                                                                  GTTATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCG                    <  SRR19973071.1011104/150‑1 (MQ=60)
                                                                                                                                     ATTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCC                 >  SRR19973071.4239630/1‑150 (MQ=60)
                                                                                                                                      TTTGTTTCGCCAGCAACCTTTATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGTTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCG                <  SRR19973071.3183333/150‑1 (MQ=60)
                                                                                                                                      TTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGACCAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCG                <  SRR19973071.1040605/150‑1 (MQ=60)
                                                                                                                                      TTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCG                <  SRR19973071.3183583/150‑1 (MQ=60)
                                                                                                                                      TTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCG                <  SRR19973071.3586428/150‑1 (MQ=60)
                                                                                                                                      TTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCG                <  SRR19973071.1190191/150‑1 (MQ=60)
                                                                                                                                      TTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCG                <  SRR19973071.1360318/150‑1 (MQ=60)
                                                                                                                                      TTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCG                <  SRR19973071.3183530/150‑1 (MQ=60)
                                                                                                                                      TTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCG                <  SRR19973071.3183339/150‑1 (MQ=60)
                                                                                                                                      TTTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCG                <  SRR19973071.3183332/150‑1 (MQ=60)
                                                                                                                                       TTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGG               <  SRR19973071.463580/150‑1 (MQ=60)
                                                                                                                                       TTGTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGG               <  SRR19973071.463030/150‑1 (MQ=60)
                                                                                                                                         GTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGAC             <  SRR19973071.1038252/150‑1 (MQ=60)
                                                                                                                                         GTTTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGAC             >  SRR19973071.2139762/1‑150 (MQ=60)
                                                                                                                                           TTCGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTG           <  SRR19973071.1773538/150‑1 (MQ=60)
                                                                                                                                             CGCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCG         <  SRR19973071.1373133/150‑1 (MQ=60)
                                                                                                                                              GCCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGA        >  SRR19973071.2393274/1‑150 (MQ=60)
                                                                                                                                               CCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAA       <  SRR19973071.641397/150‑1 (MQ=60)
                                                                                                                                               CCAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAA       <  SRR19973071.641743/150‑1 (MQ=60)
                                                                                                                                                CAGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAA      >  SRR19973071.3189752/1‑150 (MQ=60)
                                                                                                                                                 AGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAAT     >  SRR19973071.2938731/1‑150 (MQ=60)
                                                                                                                                                 AGCAACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAAT     >  SRR19973071.2939012/1‑150 (MQ=60)
                                                                                                                                                    AACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTAT  >  SRR19973071.3113229/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TTATTCTCGCCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTAT  >  CP009273/1261662‑1261959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑