Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,264,442 G→T 7.9% V274V (GTG→GTT)  kdsA → 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,264,4420GT 7.9% 1076.8 / 41.9 285V274V (GTG→GTT) kdsA3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (104/158);  new base T (13/10);  total (117/168)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

CACGAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATT  >  CP009273/1264294‑1264591
                                                                                                                                                    |                                                                                                                                                     
 aCGAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGaa                                                                                                                                                     <  2:4223598/150‑1 (MQ=255)
     gCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGt                                                                                                                                                 <  1:300515/150‑1 (MQ=255)
      ccGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGtt                                                                                                                                                <  1:1257087/150‑1 (MQ=255)
       cGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGttt                                                                                                                                               <  1:1212804/150‑1 (MQ=255)
        ggTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTc                                                                                                                                              <  1:2668631/150‑1 (MQ=255)
        ggTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTc                                                                                                                                              <  1:4007318/150‑1 (MQ=255)
        ggTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTc                                                                                                                                              >  1:324181/1‑150 (MQ=255)
            tGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCgaag                                                                                                                                          <  2:3001297/150‑1 (MQ=255)
             ggCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCgaaga                                                                                                                                         <  2:3674066/150‑1 (MQ=255)
             ggCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCgaaga                                                                                                                                         <  1:1089715/150‑1 (MQ=255)
               cGGTAGGTCTGGCGGGGCTGTTTATTGAATCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAAc                                                                                                                                       >  1:2090079/1‑150 (MQ=255)
                ggTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACt                                                                                                                                      <  1:3516844/150‑1 (MQ=255)
                 gTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTg                                                                                                                                     <  1:3699863/150‑1 (MQ=255)
                           cGGGGCTGTTTATTGAGGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCa                                                                                                                           >  1:858571/1‑150 (MQ=255)
                           cGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCa                                                                                                                           >  1:3176741/1‑150 (MQ=255)
                            ggggCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCaa                                                                                                                          <  1:1404538/150‑1 (MQ=255)
                             gggCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAg                                                                                                                         >  2:2002057/1‑150 (MQ=255)
                               gCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTa                                                                                                                       <  2:693281/150‑1 (MQ=255)
                               gCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTa                                                                                                                       <  1:1449102/150‑1 (MQ=255)
                                cTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTaa                                                                                                                      <  1:2740063/150‑1 (MQ=255)
                                 tGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAAt                                                                                                                     <  1:4521664/150‑1 (MQ=255)
                                  gTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATc                                                                                                                    >  1:1021633/1‑150 (MQ=255)
                                  gTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATc                                                                                                                    >  1:4137915/1‑150 (MQ=255)
                                  gTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATc                                                                                                                    >  2:3874627/1‑150 (MQ=255)
                                  gTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATc                                                                                                                    <  2:4534446/150‑1 (MQ=255)
                                   tttATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCt                                                                                                                   <  2:818641/150‑1 (MQ=255)
                                     tATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCttt                                                                                                                 <  2:1932026/150‑1 (MQ=255)
                                     tATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTACCTCAAGCAGATGAAAGCGAGTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCttt                                                                                                                 >  1:1531903/1‑150 (MQ=255)
                                      aTTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCtttt                                                                                                                >  2:634368/1‑150 (MQ=255)
                                       ttGAAGCGCATCCGGATCCGGAACATGCGTAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCttttt                                                                                                               <  2:4027279/150‑1 (MQ=255)
                                       ttGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCttttt                                                                                                               >  1:814968/1‑150 (MQ=255)
                                         gAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTg                                                                                                             <  2:157771/150‑1 (MQ=255)
                                          aaGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGc                                                                                                            <  1:1847829/150‑1 (MQ=255)
                                           aGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCt                                                                                                           <  2:2781191/150‑1 (MQ=255)
                                           aGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCt                                                                                                           <  2:3804151/150‑1 (MQ=255)
                                            gcgcATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCtt                                                                                                          <  2:2548363/150‑1 (MQ=255)
                                            gcgcATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCtt                                                                                                          <  2:1574032/150‑1 (MQ=255)
                                               cATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGaa                                                                                                       >  1:4249834/1‑150 (MQ=255)
                                                atccggatccggAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGaaa                                                                                                      >  1:2494824/1‑150 (MQ=255)
                                                  ccggatccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGaaaaa                                                                                                    >  1:3099899/1‑150 (MQ=255)
                                                  ccggatccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGaaaaa                                                                                                    >  2:295507/1‑150 (MQ=255)
                                                   cggatccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAAt                                                                                                   >  1:1789228/1‑150 (MQ=255)
                                                     gatccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGTAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATaa                                                                                                 <  2:1247554/150‑1 (MQ=255)
                                                     gatccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATaa                                                                                                 <  1:2211435/150‑1 (MQ=255)
                                                     gatccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATaa                                                                                                 <  2:3021976/150‑1 (MQ=255)
                                                     gatccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATaa                                                                                                 <  2:1716436/150‑1 (MQ=255)
                                                      atccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATaaa                                                                                                >  1:2420709/1‑150 (MQ=255)
                                                       tccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAg                                                                                               >  2:3157133/1‑150 (MQ=255)
                                                         cggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTa                                                                                             <  1:2711276/150‑1 (MQ=255)
                                                          ggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTAt                                                                                            >  2:2113571/1‑150 (MQ=255)
                                                          ggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTAt                                                                                            >  1:4100421/1‑150 (MQ=255)
                                                          ggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTAt                                                                                            <  1:2689928/150‑1 (MQ=255)
                                                          ggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTAt                                                                                            >  2:4441025/1‑150 (MQ=255)
                                                             aCATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAg                                                                                         <  2:3050297/150‑1 (MQ=255)
                                                                tGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGt                                                                                      <  2:2359006/150‑1 (MQ=255)
                                                                tGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGt                                                                                      <  2:926851/150‑1 (MQ=255)
                                                                tGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGt                                                                                      <  2:120682/150‑1 (MQ=255)
                                                                 gCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt                                                                                     <  2:3775092/150‑1 (MQ=255)
                                                                  cGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTc                                                                                    <  1:3435449/150‑1 (MQ=255)
                                                                   gAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTGTTTGCTTGAAAAATAAAGTATTAGCGTTCt                                                                                   <  1:1952791/150‑1 (MQ=255)
                                                                    aattgtgATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTg                                                                                  <  2:1831664/147‑1 (MQ=255)
                                                                    aaaTGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTg                                                                                  <  2:3423131/150‑1 (MQ=255)
                                                                    aaaTGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTg                                                                                  >  1:4292088/1‑150 (MQ=255)
                                                                         tgATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTa                                                                             <  2:1322502/150‑1 (MQ=255)
                                                                              gTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGAGTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACt                                                                        >  2:1063610/1‑150 (MQ=255)
                                                                                 cATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACtttt                                                                     >  1:1389503/1‑150 (MQ=255)
                                                                                  aTCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACttttt                                                                    >  1:4599443/1‑150 (MQ=255)
                                                                                   tCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACtttttt                                                                   <  1:1014799/150‑1 (MQ=255)
                                                                                     cgcgCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCa                                                                 >  1:748690/1‑150 (MQ=255)
                                                                                      gcgcTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGATAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCAt                                                                <  1:2679931/150‑1 (MQ=255)
                                                                                      gcgcTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCAt                                                                <  1:2928677/150‑1 (MQ=255)
                                                                                       cgcTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATg                                                               >  2:3957498/1‑150 (MQ=255)
                                                                                             cGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGACAGGTTTCGTAGTACTGGATACCAGCAAGTAATCTTGTTTGCTTGAAAATTAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGcc                                                         >  1:1697303/1‑150 (MQ=255)
                                                                                                 ggCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGAt                                                     >  1:2616240/1‑150 (MQ=255)
                                                                                                     aaaCTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACaa                                                 <  2:3829808/150‑1 (MQ=255)
                                                                                                      aaCTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACaaa                                                >  1:2960249/1‑150 (MQ=255)
                                                                                                              ccGGTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCg                                        <  2:3939457/150‑1 (MQ=255)
                                                                                                               cGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGAGACAAAAAAGGCCGc                                       >  1:900785/1‑150 (MQ=255)
                                                                                                                ggtCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGAAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGAGACAAAATAGGCCGCa                                      >  1:1968045/3‑150 (MQ=255)
                                                                                                                  tCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAgg                                    <  1:2565207/150‑1 (MQ=255)
                                                                                                                   ccTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGc                                   >  1:2263510/1‑150 (MQ=255)
                                                                                                                       aaGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGGACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGtt                               >  2:1673724/1‑150 (MQ=255)
                                                                                                                       aaGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGtt                               >  1:2346884/1‑150 (MQ=255)
                                                                                                                       aaGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCACGCTGtt                               <  2:1155206/150‑1 (MQ=255)
                                                                                                                             aTGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCt                         >  1:2505938/1‑150 (MQ=255)
                                                                                                                             aTGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCt                         <  2:4297077/150‑1 (MQ=255)
                                                                                                                                 aaGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCcggcgg                     >  2:1036566/1‑150 (MQ=255)
                                                                                                                                    cGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCg                  >  2:3180525/1‑150 (MQ=255)
                                                                                                                                    cGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCg                  <  2:563374/150‑1 (MQ=255)
                                                                                                                                     gATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCgg                 >  2:2255545/1‑150 (MQ=255)
                                                                                                                                      aTTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGt                >  1:2868449/1‑150 (MQ=255)
                                                                                                                                      aTTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGt                >  1:509151/1‑150 (MQ=255)
                                                                                                                                       ttGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGtt               <  2:2737356/150‑1 (MQ=255)
                                                                                                                                         gatgatCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTATCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTc             >  2:3315133/1‑150 (MQ=255)
                                                                                                                                         gatgatCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTc             <  1:688158/150‑1 (MQ=255)
                                                                                                                                            gatCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCggg          >  1:1633887/1‑150 (MQ=255)
                                                                                                                                             atCGGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGgc         <  1:609252/150‑1 (MQ=255)
                                                                                                                                               cTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGgcgc       <  1:3471744/150‑1 (MQ=255)
                                                                                                                                                tGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCa      <  1:2059277/150‑1 (MQ=255)
                                                                                                                                                 ggTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCat     >  2:233554/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CACGAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATT  >  CP009273/1264294‑1264591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A