Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,264,442 G→T 100% V274V (GTG→GTT)  kdsA → 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,264,4420GT97.8% 703.9 / 4.4 222V274V (GTG→GTT) kdsA3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base T (95/122);  total (98/124)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 222 total aligned reads displayed.

AGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGC  >  CP009273/1264298‑1264586
                                                                                                                                                |                                                                                                                                                
 gCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAg                                                                                                                                              <  1:1235190/148‑1 (MQ=255)
  ccGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAgg                                                                                                                                             <  2:1503518/148‑1 (MQ=255)
  ccGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAgg                                                                                                                                             <  2:3458248/148‑1 (MQ=255)
        tGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCga                                                                                                                                       <  1:2450346/148‑1 (MQ=255)
           cGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCgaaga                                                                                                                                    >  2:958533/1‑148 (MQ=255)
           cGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCgaaga                                                                                                                                    <  1:2788765/148‑1 (MQ=255)
            ggTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCgaagaa                                                                                                                                   <  2:1496450/148‑1 (MQ=255)
                ggTCTGGCGGGGCTGTTTATTGAATCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTgg                                                                                                                               >  1:2349672/1‑148 (MQ=255)
                   cTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATa                                                                                                                            <  2:771198/148‑1 (MQ=255)
                   cTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGGTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATa                                                                                                                            <  2:2189331/148‑1 (MQ=255)
                    tGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATAc                                                                                                                           <  1:578853/148‑1 (MQ=255)
                    tGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATAc                                                                                                                           <  1:1521913/148‑1 (MQ=255)
                    tGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATAc                                                                                                                           <  2:2031568/148‑1 (MQ=255)
                    tGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATAc                                                                                                                           <  2:119791/148‑1 (MQ=255)
                       cGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAg                                                                                                                        >  1:777942/1‑148 (MQ=255)
                       cGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAg                                                                                                                        <  1:3514512/148‑1 (MQ=255)
                       cGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAg                                                                                                                        >  1:2950013/1‑148 (MQ=255)
                        ggggCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGc                                                                                                                       <  1:1268547/148‑1 (MQ=255)
                        ggggCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGc                                                                                                                       <  2:500179/148‑1 (MQ=255)
                        ggggCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGc                                                                                                                       <  2:1993718/148‑1 (MQ=255)
                          ggCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCaa                                                                                                                     >  1:1864784/1‑148 (MQ=255)
                            cTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGt                                                                                                                   <  2:1732278/148‑1 (MQ=255)
                             tGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTa                                                                                                                  <  2:1930200/148‑1 (MQ=255)
                              gTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTaa                                                                                                                 <  1:2104962/148‑1 (MQ=255)
                              gTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTaa                                                                                                                 >  2:2001012/1‑148 (MQ=255)
                               tttATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAAt                                                                                                                >  2:2279642/1‑148 (MQ=255)
                               tttATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAAt                                                                                                                <  1:2330243/148‑1 (MQ=255)
                                   ttGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCttt                                                                                                            >  2:1995832/1‑148 (MQ=255)
                                   ttGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCttt                                                                                                            <  2:2537370/148‑1 (MQ=255)
                                   ttGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCttt                                                                                                            <  2:3012194/148‑1 (MQ=255)
                                   ttGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCttt                                                                                                            <  2:1680959/148‑1 (MQ=255)
                                    tGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCtttt                                                                                                           <  1:1981555/148‑1 (MQ=255)
                                     gAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCttttt                                                                                                          <  2:315448/148‑1 (MQ=255)
                                      aaGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCtttttt                                                                                                         >  2:2716580/1‑148 (MQ=255)
                                        gcgcATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGc                                                                                                       >  1:1325560/1‑148 (MQ=255)
                                        gcgcATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGc                                                                                                       >  1:2636059/1‑148 (MQ=255)
                                         cgcATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCt                                                                                                      <  2:397521/148‑1 (MQ=255)
                                           cATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTg                                                                                                    >  2:2591573/1‑148 (MQ=255)
                                              ccggatccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGaaa                                                                                                 >  1:31640/1‑148 (MQ=255)
                                               cggatccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATTATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGaaaa                                                                                                <  2:2952321/148‑1 (MQ=255)
                                               cggatccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGaaaa                                                                                                <  1:491573/148‑1 (MQ=255)
                                                 gatccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAAt                                                                                              >  2:3522239/1‑148 (MQ=255)
                                                  atccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATa                                                                                             >  1:3110107/1‑148 (MQ=255)
                                                  atccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATa                                                                                             <  1:2686331/148‑1 (MQ=255)
                                                      ggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGt                                                                                         <  2:286176/148‑1 (MQ=255)
                                                       gaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTa                                                                                        <  2:64865/148‑1 (MQ=255)
                                                         aCATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTAtt                                                                                      <  1:2618266/148‑1 (MQ=255)
                                                          cATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTa                                                                                     <  1:550638/148‑1 (MQ=255)
                                                           aTGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAg                                                                                    >  2:2794447/1‑148 (MQ=255)
                                                              cGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGt                                                                                 >  2:3362323/1‑148 (MQ=255)
                                                                aaaTGTGATGGTCCAGCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTc                                                                               <  2:1842013/148‑1 (MQ=255)
                                                                  aTGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTg                                                                             >  2:1219454/1‑148 (MQ=255)
                                                                  aTGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTg                                                                             <  1:333786/148‑1 (MQ=255)
                                                                  aTGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTg                                                                             <  1:3036709/148‑1 (MQ=255)
                                                                   tgtgATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGc                                                                            >  1:1047569/1‑148 (MQ=255)
                                                                    gtgATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCg                                                                           <  1:3265020/148‑1 (MQ=255)
                                                                    gtgATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCg                                                                           >  1:1928606/1‑148 (MQ=255)
                                                                           tCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGAc                                                                    >  1:1396974/1‑148 (MQ=255)
                                                                           tCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGAc                                                                    >  1:3131758/1‑148 (MQ=255)
                                                                              aTCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACttt                                                                 <  2:2104580/148‑1 (MQ=255)
                                                                              aTCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAAAAAAGTATTAGCGTTCTGCGTTAAGACttt                                                                 >  1:1030319/1‑148 (MQ=255)
                                                                               tCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACtttt                                                                <  1:2270335/148‑1 (MQ=255)
                                                                                  gcgcTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTc                                                             <  2:2359620/148‑1 (MQ=255)
                                                                                   cgcTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCa                                                            >  2:1737210/1‑148 (MQ=255)
                                                                                       gCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATggg                                                        <  1:3362323/148‑1 (MQ=255)
                                                                                       gCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATggg                                                        <  2:2557478/148‑1 (MQ=255)
                                                                                         cGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTg                                                      <  1:314677/148‑1 (MQ=255)
                                                                                            tGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCg                                                   <  2:1582832/148‑1 (MQ=255)
                                                                                            tGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCg                                                   <  2:470324/148‑1 (MQ=255)
                                                                                             ggCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCgg                                                  <  1:2838314/148‑1 (MQ=255)
                                                                                               cTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCTTGGGTGCCGGag                                                >  1:2712758/1‑147 (MQ=255)
                                                                                               cTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGAt                                                >  2:3122969/1‑148 (MQ=255)
                                                                                                    cTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGCTACaaa                                           >  2:3448027/1‑148 (MQ=255)
                                                                                                     tGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACaaaa                                          <  2:1037561/148‑1 (MQ=255)
                                                                                                       gAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACaaaaaa                                        >  2:688850/1‑148 (MQ=255)
                                                                                                       gAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACaaaaaa                                        >  2:298488/1‑148 (MQ=255)
                                                                                                          ccGTTCCTCAAGCATATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATATTTTTTGCTTTAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGc                                     <  1:1245514/148‑1 (MQ=255)
                                                                                                          ccGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGc                                     >  2:2709964/1‑148 (MQ=255)
                                                                                                              tCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCa                                 >  1:979751/1‑148 (MQ=255)
                                                                                                              tCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCa                                 >  2:1938424/1‑148 (MQ=255)
                                                                                                                cTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAgg                               >  1:623046/1‑148 (MQ=255)
                                                                                                                  cAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCt                             >  2:245170/1‑148 (MQ=255)
                                                                                                                    aGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGt                           >  2:2232341/1‑148 (MQ=255)
                                                                                                                    aGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGt                           >  2:903450/1‑148 (MQ=255)
                                                                                                                     gCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAATAGGCCGCAGGCTGtt                          >  2:1122698/1‑148 (MQ=255)
                                                                                                                        gATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTAcc                       >  1:3078663/1‑148 (MQ=255)
                                                                                                                         aTGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTAccc                      >  1:1634183/1‑148 (MQ=255)
                                                                                                                          tGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTAcccc                     <  2:3039133/148‑1 (MQ=255)
                                                                                                                           gAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCt                    >  1:2978446/1‑148 (MQ=255)
                                                                                                                            aaaGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTg                   >  1:1169569/1‑148 (MQ=255)
                                                                                                                            aaaGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTg                   >  1:1007480/1‑148 (MQ=255)
                                                                                                                             aaGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGc                  >  2:1432498/1‑148 (MQ=255)
                                                                                                                               gCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCgg                <  1:1392508/148‑1 (MQ=255)
                                                                                                                               gCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCgg                <  1:425262/148‑1 (MQ=255)
                                                                                                                                   ttGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCgg            >  1:1748285/1‑148 (MQ=255)
                                                                                                                                   ttGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCgg            >  2:1479738/1‑148 (MQ=255)
                                                                                                                                     gatgatCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGtt          <  2:2372635/148‑1 (MQ=255)
                                                                                                                                         atCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCgg      >  1:2075288/1‑148 (MQ=255)
                                                                                                                                          tCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCggg     >  1:253507/1‑148 (MQ=255)
                                                                                                                                            tGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGgcg   >  1:1477180/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGC  >  CP009273/1264298‑1264586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATAT  >  CP009273/1264298‑1264590
                                                                                                                                                |                                                                                                                                                    
AGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGG                                                                                                                                                 <  SRR19973067.1580929/150‑1 (MQ=60)
 GCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGT                                                                                                                                                <  SRR19973067.1243477/150‑1 (MQ=60)
        TGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAG                                                                                                                                         <  SRR19973067.2466450/150‑1 (MQ=60)
           CGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAAC                                                                                                                                      <  SRR19973067.2806754/150‑1 (MQ=60)
           CGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAAC                                                                                                                                      <  SRR19973067.1222941/150‑1 (MQ=60)
            GGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACT                                                                                                                                     >  SRR19973067.1044702/1‑150 (MQ=60)
            GGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACT                                                                                                                                     <  SRR19973067.1791723/150‑1 (MQ=60)
            GGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACT                                                                                                                                     >  SRR19973067.3517023/1‑150 (MQ=60)
              TAGGTCTGGCGGGGCTGTTTATTGAATCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGG                                                                                                                                   >  SRR19973067.2365015/1‑150 (MQ=60)
              TAGGTCTGGCGGGGCTGTTTATTGAATCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGG                                                                                                                                   >  SRR19973067.565981/1‑150 (MQ=60)
                GGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGAT                                                                                                                                 <  SRR19973067.592176/150‑1 (MQ=60)
                   CTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACC                                                                                                                              >  SRR19973067.3475703/1‑150 (MQ=60)
                    TGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCA                                                                                                                             <  SRR19973067.582574/150‑1 (MQ=60)
                    TGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCA                                                                                                                             <  SRR19973067.1532113/150‑1 (MQ=60)
                     GGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAG                                                                                                                            >  SRR19973067.2968679/1‑150 (MQ=60)
                     GGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAG                                                                                                                            >  SRR19973067.783333/1‑150 (MQ=60)
                      GCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGTACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGC                                                                                                                           <  SRR19973067.1469311/150‑1 (MQ=60)
                      GCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGC                                                                                                                           <  SRR19973067.670789/150‑1 (MQ=60)
                       CGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCA                                                                                                                          <  SRR19973067.3536762/150‑1 (MQ=60)
                        GGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAA                                                                                                                         >  SRR19973067.1877506/1‑150 (MQ=60)
                        GGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAA                                                                                                                         <  SRR19973067.3058736/150‑1 (MQ=60)
                        GGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAA                                                                                                                         <  SRR19973067.1277168/150‑1 (MQ=60)
                         GGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAG                                                                                                                        <  SRR19973067.2091401/150‑1 (MQ=60)
                         GGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAG                                                                                                                        <  SRR19973067.1724974/150‑1 (MQ=60)
                           GCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTA                                                                                                                      <  SRR19973067.3040443/150‑1 (MQ=60)
                              GTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATC                                                                                                                   <  SRR19973067.2118932/150‑1 (MQ=60)
                               TTTATTGAAGCGCATCCGGATCCGGAACATGCGAATTGTGATGTTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGATAGAGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCT                                                                                                                  <  SRR19973067.2345077/150‑1 (MQ=60)
                               TTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCT                                                                                                                  <  SRR19973067.2345534/150‑1 (MQ=60)
                               TTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCT                                                                                                                  <  SRR19973067.1087120/150‑1 (MQ=60)
                                    TGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTT                                                                                                             <  SRR19973067.2167774/150‑1 (MQ=60)
                                    TGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTT                                                                                                             >  SRR19973067.267849/1‑150 (MQ=60)
                                    TGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTT                                                                                                             <  SRR19973067.1994965/150‑1 (MQ=60)
                                    TGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTT                                                                                                             <  SRR19973067.1994961/150‑1 (MQ=60)
                                      AAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGC                                                                                                           >  SRR19973067.1334610/1‑150 (MQ=60)
                                      AAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGC                                                                                                           >  SRR19973067.1334607/1‑150 (MQ=60)
                                      AAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGC                                                                                                           >  SRR19973067.2653113/1‑150 (MQ=60)
                                       AGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCT                                                                                                          >  SRR19973067.473580/1‑150 (MQ=60)
                                         CGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTG                                                                                                        <  SRR19973067.3370065/150‑1 (MQ=60)
                                            ATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAA                                                                                                     >  SRR19973067.32278/1‑150 (MQ=60)
                                            ATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAA                                                                                                     >  SRR19973067.32113/1‑150 (MQ=60)
                                            ATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAA                                                                                                     >  SRR19973067.32100/1‑150 (MQ=60)
                                               CGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAAT                                                                                                  <  SRR19973067.494955/150‑1 (MQ=60)
                                                GGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATA                                                                                                 >  SRR19973067.3129888/1‑150 (MQ=60)
                                                GGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATA                                                                                                 >  SRR19973067.3129910/1‑150 (MQ=60)
                                                  cgtgtgctcttccgatcTGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAA                                                                                               <  SRR19973067.2357668/133‑1 (MQ=60)
                                                  ATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAA                                                                                               <  SRR19973067.2704306/150‑1 (MQ=60)
                                                  ATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAA                                                                                               <  SRR19973067.1031309/150‑1 (MQ=60)
                                                  ATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAA                                                                                               <  SRR19973067.2703565/150‑1 (MQ=60)
                                                  ATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAA                                                                                               <  SRR19973067.1252892/150‑1 (MQ=60)
                                                   TCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAG                                                                                              <  SRR19973067.33569/150‑1 (MQ=60)
                                                   TCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAG                                                                                              <  SRR19973067.1845718/150‑1 (MQ=60)
                                                       GAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATT                                                                                          <  SRR19973067.2383616/150‑1 (MQ=60)
                                                         ACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAG                                                                                        <  SRR19973067.2635247/150‑1 (MQ=60)
                                                         ACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAG                                                                                        <  SRR19973067.2635272/150‑1 (MQ=60)
                                                         ACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAG                                                                                        >  SRR19973067.2997154/1‑150 (MQ=60)
                                                          CATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTACTCTTTTTTGCTTGAAAAATAAAGTATTAGC                                                                                       >  SRR19973067.544611/1‑150 (MQ=60)
                                                          CATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGC                                                                                       <  SRR19973067.554266/150‑1 (MQ=60)
                                                               GAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCT                                                                                  <  SRR19973067.79657/150‑1 (MQ=60)
                                                                 AATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCaga                                                                                >  SRR19973067.3024803/1‑147 (MQ=60)
                                                                 AATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGC                                                                                >  SRR19973067.1054762/1‑150 (MQ=60)
                                                                  ATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCG                                                                               <  SRR19973067.336072/150‑1 (MQ=60)
                                                                  ATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCG                                                                               >  SRR19973067.1941765/1‑150 (MQ=60)
                                                                  ATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCG                                                                               <  SRR19973067.3056055/150‑1 (MQ=60)
                                                                    GTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTT                                                                             <  SRR19973067.1508474/150‑1 (MQ=60)
                                                                    GTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTT                                                                             <  SRR19973067.3285400/150‑1 (MQ=60)
                                                                       ATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAG                                                                          >  SRR19973067.2559248/1‑150 (MQ=60)
                                                                        TGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGA                                                                         <  SRR19973067.281329/150‑1 (MQ=60)
                                                                         GGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGAC                                                                        >  SRR19973067.3151664/1‑150 (MQ=60)
                                                                         GGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGAC                                                                        >  SRR19973067.3151657/1‑150 (MQ=60)
                                                                         GGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGAC                                                                        >  SRR19973067.1406460/1‑150 (MQ=60)
                                                                          GTCCATCCGCGCTGCCGCTGGCTAAACTGGAACTGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACT                                                                       >  SRR19973067.2783206/1‑150 (MQ=60)
                                                                            CCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAAAAAAGTATTAGCGTTCTGCGTTAAGACTTT                                                                     >  SRR19973067.1037411/1‑150 (MQ=60)
                                                                               TCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTT                                                                  <  SRR19973067.2285375/150‑1 (MQ=60)
                                                                                       GCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTG                                                          <  SRR19973067.3383668/150‑1 (MQ=60)
                                                                                         CGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCC                                                        <  SRR19973067.316781/150‑1 (MQ=60)
                                                                                            TGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGA                                                     >  SRR19973067.1886888/1‑150 (MQ=60)
                                                                                             GGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCTTGGGTGCCGGAG                                                    >  SRR19973067.2730241/1‑150 (MQ=60)
                                                                                             GGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGAT                                                    <  SRR19973067.2856668/150‑1 (MQ=60)
                                                                                             GGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGAT                                                    <  SRR19973067.2856544/150‑1 (MQ=60)
                                                                                                        AACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGC                                         <  SRR19973067.3169630/150‑1 (MQ=60)
                                                                                                          CCGTTCCTCAAGCATATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATATTTTTTGCTTTAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCG                                       <  SRR19973067.1253915/150‑1 (MQ=60)
                                                                                                          CCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCG                                       <  SRR19973067.1253940/150‑1 (MQ=60)
                                                                                                          CCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCG                                       <  SRR19973067.1253705/150‑1 (MQ=60)
                                                                                                            GTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCA                                     >  SRR19973067.986478/1‑150 (MQ=60)
                                                                                                              TCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGG                                   >  SRR19973067.627157/1‑150 (MQ=60)
                                                                                                              TCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGG                                   >  SRR19973067.2533600/1‑150 (MQ=60)
                                                                                                              TCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGG                                   >  SRR19973067.2533613/1‑150 (MQ=60)
                                                                                                                   AAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTT                              >  SRR19973067.1460073/1‑150 (MQ=60)
                                                                                                                      CAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACC                           >  SRR19973067.3098254/1‑150 (MQ=60)
                                                                                                                       AGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCC                          >  SRR19973067.1645422/1‑150 (MQ=60)
                                                                                                                         ATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCT                        >  SRR19973067.2997253/1‑150 (MQ=60)
                                                                                                                          TGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTG                       >  SRR19973067.1177326/1‑150 (MQ=60)
                                                                                                                          TGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTG                       >  SRR19973067.1014402/1‑150 (MQ=60)
                                                                                                                            AAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCG                     <  SRR19973067.2766223/150‑1 (MQ=60)
                                                                                                                               GCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCC                  <  SRR19973067.428220/150‑1 (MQ=60)
                                                                                                                               GCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCC                  <  SRR19973067.3121997/150‑1 (MQ=60)
                                                                                                                               GCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCC                  <  SRR19973067.1401977/150‑1 (MQ=60)
                                                                                                                               GCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCC                  <  SRR19973067.1401969/150‑1 (MQ=60)
                                                                                                                                CGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCG                 >  SRR19973067.593773/1‑150 (MQ=60)
                                                                                                                                 GATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGG                >  SRR19973067.1760207/1‑150 (MQ=60)
                                                                                                                                  ATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGT               <  SRR19973067.2014508/150‑1 (MQ=60)
                                                                                                                                     GATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTC            >  SRR19973067.398739/1‑150 (MQ=60)
                                                                                                                                       TGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGG          >  SRR19973067.2089064/1‑150 (MQ=60)
                                                                                                                                        GATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGG         >  SRR19973067.2250229/1‑150 (MQ=60)
                                                                                                                                        GATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGG         >  SRR19973067.255245/1‑150 (MQ=60)
                                                                                                                                         ATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGC        <  SRR19973067.131738/150‑1 (MQ=60)
                                                                                                                                          TCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCG       >  SRR19973067.1487001/1‑150 (MQ=60)
                                                                                                                                               TTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATAT  >  SRR19973067.1997100/1‑150 (MQ=60)
                                                                                                                                               TTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATAT  >  SRR19973067.1997006/1‑150 (MQ=60)
                                                                                                                                                |                                                                                                                                                    
AGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATAT  >  CP009273/1264298‑1264590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑