Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,264,442 G→T V274V (GTG→GTT)  kdsA → 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,264,4420GT100.0% 616.4 / NA 183V274V (GTG→GTT) kdsA3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (86/97);  total (86/97)

Only 100 of 183 total aligned reads displayed.

GAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCAT  >  CP009273/1264297‑1264588
                                                                                                                                                 |                                                                                                                                                  
   ccGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGtt                                                                                                                                             <  2:555005/150‑1 (MQ=255)
        aTGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCgaa                                                                                                                                        >  1:935963/1‑150 (MQ=255)
         tGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCgaag                                                                                                                                       <  1:287052/150‑1 (MQ=255)
           gCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCgaagaa                                                                                                                                     <  2:110237/150‑1 (MQ=255)
             ggTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGATCATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACt                                                                                                                                   <  1:2134728/150‑1 (MQ=255)
              gTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTg                                                                                                                                  <  1:546555/150‑1 (MQ=255)
               tAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTgg                                                                                                                                 >  1:1686900/1‑150 (MQ=255)
                aGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGa                                                                                                                                >  2:803671/1‑150 (MQ=255)
                aGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGa                                                                                                                                >  2:1735408/1‑150 (MQ=255)
                 ggTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGAt                                                                                                                               <  1:652155/150‑1 (MQ=255)
                 ggTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGAt                                                                                                                               <  1:2558089/150‑1 (MQ=255)
                 ggTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGAt                                                                                                                               <  2:3580592/150‑1 (MQ=255)
                 ggTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGAt                                                                                                                               <  1:1362866/150‑1 (MQ=255)
                     tGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCa                                                                                                                           <  2:669176/150‑1 (MQ=255)
                      ggCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAg                                                                                                                          <  2:41520/150‑1 (MQ=255)
                       gCGGGGCTGTTTATTGAAGCGCTTCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGGTCCTCAAGCAGTTGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGc                                                                                                                         <  2:2821083/150‑1 (MQ=255)
                       gCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGc                                                                                                                         <  1:2350326/150‑1 (MQ=255)
                         ggggCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCaa                                                                                                                       <  1:2090422/150‑1 (MQ=255)
                         ggggCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCaa                                                                                                                       <  1:1998070/150‑1 (MQ=255)
                          gggCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAg                                                                                                                      <  1:3791288/150‑1 (MQ=255)
                             cTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTaa                                                                                                                   <  1:432113/150‑1 (MQ=255)
                              tGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAAt                                                                                                                  >  1:2408088/1‑150 (MQ=255)
                               gTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTTATc                                                                                                                 <  1:207791/150‑1 (MQ=255)
                               gTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATc                                                                                                                 <  2:666379/150‑1 (MQ=255)
                                  tATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCttt                                                                                                              >  1:1552341/1‑150 (MQ=255)
                                     tGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCtttttt                                                                                                           <  1:2301474/150‑1 (MQ=255)
                                     tGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCtttttt                                                                                                           <  2:1422548/150‑1 (MQ=255)
                                     tGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCtttttt                                                                                                           <  2:3469515/150‑1 (MQ=255)
                                      gacgcgcATCCGGATCCGGAACAGGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTg                                                                                                          <  2:3270306/147‑1 (MQ=255)
                                      gAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTg                                                                                                          <  1:3416038/150‑1 (MQ=255)
                                      gAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTg                                                                                                          <  1:1441876/150‑1 (MQ=255)
                                        aGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCt                                                                                                        >  2:3753534/1‑150 (MQ=255)
                                         gcgcATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCtt                                                                                                       <  2:613115/150‑1 (MQ=255)
                                                cggatccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAAt                                                                                                <  1:1737645/150‑1 (MQ=255)
                                                 ggatccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATa                                                                                               <  2:3774007/150‑1 (MQ=255)
                                                   atccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATaaa                                                                                             <  2:3758931/150‑1 (MQ=255)
                                                     ccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGt                                                                                           <  2:2072228/150‑1 (MQ=255)
                                                     ccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGt                                                                                           <  2:1465941/150‑1 (MQ=255)
                                                        gaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTAtt                                                                                        <  2:1171587/150‑1 (MQ=255)
                                                            aTGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTGAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCg                                                                                    >  2:3397400/1‑150 (MQ=255)
                                                            aTGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCg                                                                                    >  2:878271/1‑150 (MQ=255)
                                                            aTGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCg                                                                                    <  1:2176861/150‑1 (MQ=255)
                                                                gAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCt                                                                                >  1:1328851/1‑150 (MQ=255)
                                                                gAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCt                                                                                <  2:2166637/150‑1 (MQ=255)
                                                                gAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCt                                                                                >  1:962232/1‑150 (MQ=255)
                                                                    tgtgATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGt                                                                            >  2:1131641/1‑150 (MQ=255)
                                                                    tgtgATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGt                                                                            >  2:2991618/1‑150 (MQ=255)
                                                                     gtgATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGNGtt                                                                           <  1:2371149/150‑1 (MQ=255)
                                                                      tgATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTa                                                                          >  2:2497817/1‑150 (MQ=255)
                                                                           gTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACt                                                                     >  2:1626430/1‑150 (MQ=255)
                                                                             ccATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACttt                                                                   >  1:2294548/1‑150 (MQ=255)
                                                                               aTCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACttttt                                                                 >  1:2929023/1‑150 (MQ=255)
                                                                               aTCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACttttt                                                                 <  1:735632/150‑1 (MQ=255)
                                                                               aTCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACttttt                                                                 >  1:2677122/1‑150 (MQ=255)
                                                                               aTCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACttttt                                                                 <  1:1626001/150‑1 (MQ=255)
                                                                               aTCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACttttt                                                                 <  1:2221475/150‑1 (MQ=255)
                                                                                 ccGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTAt                                                                                         >  1:3185042/1‑124 (MQ=255)
                                                                                 ccGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTAt                                                                                         <  2:3185042/124‑1 (MQ=255)
                                                                                       tGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGt                                                         <  1:1906491/150‑1 (MQ=255)
                                                                                       tGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGt                                                         <  1:3294650/150‑1 (MQ=255)
                                                                                           gCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCg                                                     <  1:868300/150‑1 (MQ=255)
                                                                                           gCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCg                                                     >  1:2722949/1‑150 (MQ=255)
                                                                                             tGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGa                                                   >  1:1127608/1‑150 (MQ=255)
                                                                                             tGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGa                                                   >  1:2604906/1‑150 (MQ=255)
                                                                                              ggCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGAt                                                  <  2:2437314/150‑1 (MQ=255)
                                                                                                 tAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTATGACTTTTTTCATGGGTGCCGGATACa                                               >  2:1390343/1‑150 (MQ=255)
                                                                                                  aaaCTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACaa                                              >  2:3292946/1‑150 (MQ=255)
                                                                                                   aaCTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACaaa                                             >  2:2142837/1‑150 (MQ=255)
                                                                                                    aCTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGAAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACaaaa                                            >  1:950227/1‑150 (MQ=255)
                                                                                                      tGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACaaaaaa                                          >  2:1719113/1‑150 (MQ=255)
                                                                                                       ggAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAg                                         >  2:2228398/1‑150 (MQ=255)
                                                                                                       ggAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAg                                         >  1:190931/1‑150 (MQ=255)
                                                                                                       ggAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAg                                         <  2:3145512/150‑1 (MQ=255)
                                                                                                        gAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAgg                                        <  1:305196/150‑1 (MQ=255)
                                                                                                        gAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAgg                                        <  1:3203815/150‑1 (MQ=255)
                                                                                                        gAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAgg                                        <  1:2215890/150‑1 (MQ=255)
                                                                                                         aaCCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGc                                       >  2:742426/1‑150 (MQ=255)
                                                                                                            cGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGc                                    >  1:2190028/1‑150 (MQ=255)
                                                                                                            cGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGc                                    >  2:2902664/1‑150 (MQ=255)
                                                                                                            cGTTCCTCAAGCAGACGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGc                                    >  2:362285/1‑150 (MQ=255)
                                                                                                              taccTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAg                                  >  2:776824/3‑150 (MQ=255)
                                                                                                                  tCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTg                              <  2:2395232/150‑1 (MQ=255)
                                                                                                                   cAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGt                             >  2:1024999/1‑150 (MQ=255)
                                                                                                                     aGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTa                           >  2:2093210/1‑150 (MQ=255)
                                                                                                                        aGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATC‑TTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTAcccc                       >  2:3605466/1‑150 (MQ=255)
                                                                                                                         gATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTAcccc                       >  1:1478081/1‑150 (MQ=255)
                                                                                                                         gATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTAGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTAcccc                       >  1:1479548/1‑150 (MQ=255)
                                                                                                                          aTGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCt                      >  1:3072471/1‑150 (MQ=255)
                                                                                                                          aTGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCt                      <  2:3026810/150‑1 (MQ=255)
                                                                                                                           tGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAATAGGCCGCAGGCTGTTACCCCTg                     >  2:1880402/1‑150 (MQ=255)
                                                                                                                            gAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGc                    >  1:2686210/1‑150 (MQ=255)
                                                                                                                            gAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGc                    >  2:2875184/1‑150 (MQ=255)
                                                                                                                             aaaGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAATGGCCGCAGGCTGTTACCCCTGCg                   >  2:3723131/1‑150 (MQ=255)
                                                                                                                              aaGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCgg                  >  2:2512414/1‑150 (MQ=255)
                                                                                                                                    ttGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGtt            <  1:2817691/150‑1 (MQ=255)
                                                                                                                                    ttGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGtt            <  1:2571941/150‑1 (MQ=255)
                                                                                                                                      gatgatCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTc          <  2:2710487/150‑1 (MQ=255)
                                                                                                                                            cTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGgcgc    >  2:1260888/1‑150 (MQ=255)
                                                                                                                                             tGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCa   >  2:1338417/1‑150 (MQ=255)
                                                                                                                                              ggTTAATGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTTAAAAATAAATTATTAGCGTTATGCGTTAAGACTTTTTTAATTGGTGCAGGATTCAAAAAAGGCCGCAGGATGTTACCCCTGCGGCCGGTTTAGGGCGCat  >  1:1037828/1‑150 (MQ=37)
                                                                                                                                                 |                                                                                                                                                  
GAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCAT  >  CP009273/1264297‑1264588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACGAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATT  >  CP009273/1264294‑1264591
                                                                                                                                                    |                                                                                                                                                     
CACGAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTA                                                                                                                                                      >  SRR19973061.2025035/1‑150 (MQ=60)
   GAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAG                                                                                                                                                   >  SRR19973061.1718386/1‑150 (MQ=60)
       CGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTT                                                                                                                                               <  SRR19973061.2842369/150‑1 (MQ=60)
       CGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTT                                                                                                                                               <  SRR19973061.447996/150‑1 (MQ=60)
           ATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAA                                                                                                                                           >  SRR19973061.938344/1‑150 (MQ=60)
           ATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAA                                                                                                                                           >  SRR19973061.2717466/1‑150 (MQ=60)
           ATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAA                                                                                                                                           <  SRR19973061.3360378/150‑1 (MQ=60)
            TGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAG                                                                                                                                          <  SRR19973061.287824/150‑1 (MQ=60)
                GGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGATCATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACT                                                                                                                                      <  SRR19973061.2140088/150‑1 (MQ=60)
                GGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACT                                                                                                                                      >  SRR19973061.989381/1‑150 (MQ=60)
                 GTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTG                                                                                                                                     <  SRR19973061.547995/150‑1 (MQ=60)
                  TAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGG                                                                                                                                    >  SRR19973061.1691139/1‑150 (MQ=60)
                    GGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCGGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGAT                                                                                                                                  <  SRR19973061.2779366/150‑1 (MQ=60)
                    GGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGAT                                                                                                                                  <  SRR19973061.653827/150‑1 (MQ=60)
                    GGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGAT                                                                                                                                  <  SRR19973061.2564617/150‑1 (MQ=60)
                    GGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGAT                                                                                                                                  <  SRR19973061.1366303/150‑1 (MQ=60)
                      TCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCGGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATAC                                                                                                                                <  SRR19973061.2566325/150‑1 (MQ=60)
                         GGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAG                                                                                                                             >  SRR19973061.953807/1‑150 (MQ=60)
                          GCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGC                                                                                                                            <  SRR19973061.2356299/150‑1 (MQ=60)
                            GGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAA                                                                                                                          <  SRR19973061.2095673/150‑1 (MQ=60)
                            GGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAA                                                                                                                          <  SRR19973061.2003072/150‑1 (MQ=60)
                             GGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAG                                                                                                                         >  SRR19973061.754006/1‑150 (MQ=60)
                             GGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAG                                                                                                                         <  SRR19973061.3800947/150‑1 (MQ=60)
                                CTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAA                                                                                                                      <  SRR19973061.433292/150‑1 (MQ=60)
                                CTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAA                                                                                                                      <  SRR19973061.2116083/150‑1 (MQ=60)
                                CTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAA                                                                                                                      <  SRR19973061.2344841/150‑1 (MQ=60)
                                 TGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAAT                                                                                                                     >  SRR19973061.2414241/1‑150 (MQ=60)
                                  GTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTTATC                                                                                                                    <  SRR19973061.208413/150‑1 (MQ=60)
                                     TATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTT                                                                                                                 >  SRR19973061.1556200/1‑150 (MQ=60)
                                     TATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTT                                                                                                                 <  SRR19973061.1558041/150‑1 (MQ=60)
                                     TATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTT                                                                                                                 >  SRR19973061.3034630/1‑150 (MQ=60)
                                       TTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTT                                                                                                               <  SRR19973061.1671450/150‑1 (MQ=60)
                                        TGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTT                                                                                                              <  SRR19973061.2307303/150‑1 (MQ=60)
                                         GAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTG                                                                                                             <  SRR19973061.3424807/150‑1 (MQ=60)
                                         GAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTG                                                                                                             <  SRR19973061.1445511/150‑1 (MQ=60)
                                          AAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGC                                                                                                            <  SRR19973061.2211096/150‑1 (MQ=60)
                                             CGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTG                                                                                                         <  SRR19973061.1466526/150‑1 (MQ=60)
                                             CGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGGTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTG                                                                                                         <  SRR19973061.2160004/150‑1 (MQ=60)
                                              GCATCCGGATCCGGAACATGCGAAATGCGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGA                                                                                                        <  SRR19973061.291438/150‑1 (MQ=60)
                                                   CGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAAT                                                                                                   <  SRR19973061.1742015/150‑1 (MQ=60)
                                                   CGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAAT                                                                                                   <  SRR19973061.1345604/150‑1 (MQ=60)
                                                      ATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAA                                                                                                >  SRR19973061.1551189/1‑150 (MQ=60)
                                                          GGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTAT                                                                                            <  SRR19973061.1829779/150‑1 (MQ=60)
                                                            AACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTA                                                                                          <  SRR19973061.208149/150‑1 (MQ=60)
                                                            AACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTA                                                                                          <  SRR19973061.208946/150‑1 (MQ=60)
                                                               ATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCG                                                                                       <  SRR19973061.2182331/150‑1 (MQ=60)
                                                                  CGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGCAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTC                                                                                    <  SRR19973061.917184/150‑1 (MQ=60)
                                                                   GAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCT                                                                                   >  SRR19973061.964660/1‑150 (MQ=60)
                                                                   GAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCT                                                                                   >  SRR19973061.1332192/1‑150 (MQ=60)
                                                                        GTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGNGTT                                                                              <  SRR19973061.2377192/150‑1 (MQ=60)
                                                                                CCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTT                                                                      >  SRR19973061.2300350/1‑150 (MQ=60)
                                                                                CCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTT                                                                      >  SRR19973061.2300054/1‑150 (MQ=60)
                                                                                  ATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTT                                                                    >  SRR19973061.2936553/1‑150 (MQ=60)
                                                                                  ATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTT                                                                    <  SRR19973061.2227049/150‑1 (MQ=60)
                                                                                  ATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTT                                                                    <  SRR19973061.1630064/150‑1 (MQ=60)
                                                                                  ATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTT                                                                    >  SRR19973061.2684010/1‑150 (MQ=60)
                                                                                  ATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTT                                                                    <  SRR19973061.737560/150‑1 (MQ=60)
                                                                                  ATCCGCGCTGCCGCTGGCTAAACTGGAACCGGTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTT                                                                    <  SRR19973061.2227046/150‑1 (MQ=60)
                                                                                   TCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTT                                                                   >  SRR19973061.2001613/1‑150 (MQ=60)
                                                                                    CCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATagatcggaagagcacacgtctgaact                                                                  >  SRR19973061.3193216/1‑124 (MQ=60)
                                                                                    CCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTC                                                                  <  SRR19973061.2380853/150‑1 (MQ=60)
                                                                                          TGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGT                                                            <  SRR19973061.1911212/150‑1 (MQ=60)
                                                                                          TGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGT                                                            <  SRR19973061.3303133/150‑1 (MQ=60)
                                                                                          TGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGT                                                            <  SRR19973061.3302616/150‑1 (MQ=60)
                                                                                              GCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCG                                                        <  SRR19973061.870551/150‑1 (MQ=60)
                                                                                              GCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCG                                                        >  SRR19973061.2729954/1‑150 (MQ=60)
                                                                                              GCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCG                                                        >  SRR19973061.574453/1‑150 (MQ=60)
                                                                                                TGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGA                                                      >  SRR19973061.1130488/1‑150 (MQ=60)
                                                                                                TGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGA                                                      >  SRR19973061.2611601/1‑150 (MQ=60)
                                                                                                     AAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAA                                                 >  SRR19973061.2542471/1‑150 (MQ=60)
                                                                                                       ACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGAAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAA                                               >  SRR19973061.952625/1‑150 (MQ=60)
                                                                                                          GGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAG                                            >  SRR19973061.191508/1‑150 (MQ=60)
                                                                                                           GAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAATAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGG                                           <  SRR19973061.3211553/150‑1 (MQ=60)
                                                                                                           GAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGG                                           <  SRR19973061.3212047/150‑1 (MQ=60)
                                                                                                           GAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGG                                           <  SRR19973061.306000/150‑1 (MQ=60)
                                                                                                           GAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGG                                           <  SRR19973061.2221454/150‑1 (MQ=60)
                                                                                                               CGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGC                                       >  SRR19973061.2195532/1‑150 (MQ=60)
                                                                                                                GTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCA                                      >  SRR19973061.2597769/1‑150 (MQ=60)
                                                                                                                   CCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGC                                   <  SRR19973061.1289656/150‑1 (MQ=60)
                                                                                                                      CAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATTGGTGCCGGATACAAAAAAGGCCGCAGGCTGT                                >  SRR19973061.1435563/1‑150 (MQ=60)
                                                                                                                      CAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGT                                >  SRR19973061.3618100/1‑150 (MQ=60)
                                                                                                                            GATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCC                          >  SRR19973061.1481792/1‑150 (MQ=60)
                                                                                                                            GATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTAGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCC                          >  SRR19973061.1483261/1‑150 (MQ=60)
                                                                                                                             ATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCT                         >  SRR19973061.3080402/1‑150 (MQ=60)
                                                                                                                              TGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTG                        >  SRR19973061.734445/1‑150 (MQ=60)
                                                                                                                              TGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTG                        >  SRR19973061.2645889/1‑150 (MQ=60)
                                                                                                                               GAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGC                       >  SRR19973061.2693120/1‑150 (MQ=60)
                                                                                                                                       TTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTT               <  SRR19973061.2578522/150‑1 (MQ=60)
                                                                                                                                       TTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTT               <  SRR19973061.2824920/150‑1 (MQ=60)
                                                                                                                                        TGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTT              >  SRR19973061.3081643/1‑150 (MQ=60)
                                                                                                                                                TGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCA      >  SRR19973061.2304470/1‑150 (MQ=60)
                                                                                                                                                 GGTTAATGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTTAAAAATAAATTATTAGCGTTATGCGTTAAGACTTTTTTAATTGGTGCAGGATTCAAAAAAGGCCGCAGGATGTTACCCCTGCGGCCGGTTTAGGGCGCAT     >  SRR19973061.1040487/1‑150 (MQ=60)
                                                                                                                                                    TAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATT  <  SRR19973061.3619131/150‑1 (MQ=60)
                                                                                                                                                    TAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATT  >  SRR19973061.2702003/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CACGAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATT  >  CP009273/1264294‑1264591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑