Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,264,442 G→T 100% V274V (GTG→GTT)  kdsA → 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,264,4420GT95.1% 601.0 / 20.7 205V274V (GTG→GTT) kdsA3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (5/5);  new base T (109/86);  total (114/91)
Fisher's exact test for biased strand distribution p-value = 7.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 205 total aligned reads displayed.

GAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGC  >  CP009273/1264297‑1264586
                                                                                                                                                 |                                                                                                                                                
gAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTaa                                                                                                                                                <  1:2922184/148‑1 (MQ=255)
gAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTaa                                                                                                                                                >  2:1840327/1‑148 (MQ=255)
gAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGGTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTaa                                                                                                                                                <  1:2922407/148‑1 (MQ=255)
 aGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTaaa                                                                                                                                               >  2:2080321/1‑148 (MQ=255)
   ccGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAgg                                                                                                                                             >  1:3233468/1‑148 (MQ=255)
      gTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGttt                                                                                                                                          <  1:1074998/148‑1 (MQ=255)
      gTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGttt                                                                                                                                          <  1:51414/148‑1 (MQ=255)
        aTGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCg                                                                                                                                        >  1:424472/1‑148 (MQ=255)
               tAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACt                                                                                                                                 <  1:81983/148‑1 (MQ=255)
                 ggTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTgg                                                                                                                               <  2:1879260/148‑1 (MQ=255)
                      ggCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATAcc                                                                                                                          >  1:636650/1‑148 (MQ=255)
                             cTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGt                                                                                                                   >  1:1528469/1‑148 (MQ=255)
                               gTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTaa                                                                                                                 <  2:382573/148‑1 (MQ=255)
                                tttATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCACGTAAt                                                                                                                >  2:1970253/1‑148 (MQ=255)
                                 ttATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATc                                                                                                               >  1:2275496/1‑148 (MQ=255)
                                  tATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCt                                                                                                              <  2:3418132/148‑1 (MQ=255)
                                   aTTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTGATCtt                                                                                                             <  1:478545/148‑1 (MQ=255)
                                   aTTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCtt                                                                                                             >  2:1183049/1‑148 (MQ=255)
                                    ttGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCttt                                                                                                            <  1:920286/148‑1 (MQ=255)
                                     tGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCtttt                                                                                                           <  2:47141/148‑1 (MQ=255)
                                         gcgcATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGc                                                                                                       <  2:318926/148‑1 (MQ=255)
                                         gcgcATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGc                                                                                                       >  1:1114325/1‑148 (MQ=255)
                                         gcgcATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGc                                                                                                       <  1:162182/148‑1 (MQ=255)
                                         gcgcATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGc                                                                                                       <  2:318653/148‑1 (MQ=255)
                                           gcATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCtt                                                                                                     <  2:133421/148‑1 (MQ=255)
                                            cATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTg                                                                                                    >  2:1468389/1‑148 (MQ=255)
                                             atccggatccggAACATGCGAATTGTGATGGTCCATCCGCGCTGCCGCTGGCTACACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGa                                                                                                   <  2:2622028/148‑1 (MQ=255)
                                             atccggatccggAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGa                                                                                                   <  2:2622321/148‑1 (MQ=255)
                                                 ggatccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGaaaaa                                                                                               >  1:1461549/1‑148 (MQ=255)
                                                 ggatccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGaaaaa                                                                                               <  1:40332/148‑1 (MQ=255)
                                                  gatccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAAt                                                                                              >  2:103498/1‑148 (MQ=255)
                                                   atccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATa                                                                                             <  1:1485124/148‑1 (MQ=255)
                                                   atccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATa                                                                                             >  2:2518134/1‑148 (MQ=255)
                                                    tccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATaa                                                                                            <  2:2634056/148‑1 (MQ=255)
                                                      cggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAg                                                                                          >  2:3473303/1‑148 (MQ=255)
                                                       ggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGt                                                                                         <  1:3078699/148‑1 (MQ=255)
                                                        gaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTa                                                                                        >  1:2366859/1‑148 (MQ=255)
                                                         aaCATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTAt                                                                                       <  1:2539489/148‑1 (MQ=255)
                                                          aCATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTAtt                                                                                      <  2:2885705/148‑1 (MQ=255)
                                                            aTGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAg                                                                                    >  2:3292528/1‑148 (MQ=255)
                                                            aTGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAg                                                                                    >  2:3292492/1‑148 (MQ=255)
                                                             tGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGc                                                                                   <  1:1703855/148‑1 (MQ=255)
                                                              gCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCg                                                                                  >  1:3340177/1‑148 (MQ=255)
                                                              gCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCg                                                                                  >  1:155814/1‑148 (MQ=255)
                                                              gCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCg                                                                                  >  1:1961017/1‑148 (MQ=255)
                                                               cGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGTACAATAACGTATTAGCGt                                                                                 >  2:2603542/1‑148 (MQ=255)
                                                                gacaggtgAGGGTCCCTCCGCGCTGCCGCTGGCTAAACTGGCACCGGGCCTCACGCAGATGAAAGCGATGGATGATCTGGTTAAAGGATTCGAAGAACTGGATCCAAGCAATTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt                                                                                <  2:2476906/143‑1 (MQ=255)
                                                                 aaaTGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTc                                                                               <  2:1739829/148‑1 (MQ=255)
                                                                   aTGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAATATAAAGTATTAGCGTTCTg                                                                             >  1:2469719/1‑148 (MQ=255)
                                                                   aTGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTg                                                                             >  1:1706993/1‑148 (MQ=255)
                                                                     gtgATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCg                                                                           >  1:2203739/1‑148 (MQ=255)
                                                                        aTGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTa                                                                        <  1:2587055/148‑1 (MQ=255)
                                                                         tGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTaa                                                                       >  1:3146601/1‑148 (MQ=255)
                                                                            tcaaTCCGCGCTGCCCCTGGCTAAACTGGACCCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGAc                                                                    >  2:760472/4‑148 (MQ=255)
                                                                            tCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGAc                                                                    >  2:2671692/1‑148 (MQ=255)
                                                                            tCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGAc                                                                    <  1:3197594/148‑1 (MQ=255)
                                                                            tCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGAc                                                                    <  1:1840327/148‑1 (MQ=255)
                                                                            tCCATCCGCGCTGCCGCGGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGAc                                                                    <  2:73739/148‑1 (MQ=255)
                                                                               aTCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACttt                                                                 <  1:2791288/148‑1 (MQ=255)
                                                                                tCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACtttt                                                                >  2:314918/1‑148 (MQ=255)
                                                                                tCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACtttt                                                                <  1:1419499/148‑1 (MQ=255)
                                                                                 ccGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACttttt                                                               >  1:64475/1‑148 (MQ=255)
                                                                                  cgcgCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACtttttt                                                              <  1:3357891/148‑1 (MQ=255)
                                                                                      cTGCCGCTGGCTTAACTGGTACCGTTCGTCATGCAGATTTTAGCGATTGATGTTCTGGTTAATGGTTTCTAAGAACTGGATAGCAGCAAGTAATCTTTTTTGCTTGTAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATg                                                          <  2:1932446/148‑1 (MQ=255)
                                                                                         ccGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGt                                                       >  1:1328306/1‑148 (MQ=255)
                                                                                          cGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTg                                                      <  2:3346936/148‑1 (MQ=255)
                                                                                                cTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATC‑TTTTTGTTTGAAAAATAAAGTCTTAGCGTTGTGCGTTAAGACTTTTTT‑ATGGGTGCCGGATAc                                              >  1:1014321/1‑148 (MQ=255)
                                                                                                    aCTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACaa                                            <  1:2685782/148‑1 (MQ=255)
                                                                                                      tGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACaaaa                                          <  1:3508493/148‑1 (MQ=255)
                                                                                                        gAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACaaaaaa                                        >  1:3272135/1‑148 (MQ=255)
                                                                                                           ccGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGc                                     >  2:2253287/1‑148 (MQ=255)
                                                                                                           ccGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGc                                     >  1:783224/1‑148 (MQ=255)
                                                                                                           ccGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGc                                     >  2:2759838/1‑148 (MQ=255)
                                                                                                              ttCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGTACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGc                                  >  1:1630842/1‑148 (MQ=255)
                                                                                                               tCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCa                                 >  1:2061316/1‑148 (MQ=255)
                                                                                                                 cTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAgg                               <  2:3349530/148‑1 (MQ=255)
                                                                                                                 cTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAgg                               <  2:1847722/148‑1 (MQ=255)
                                                                                                                   cAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCt                             >  1:3213108/1‑148 (MQ=255)
                                                                                                                   cAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCt                             >  2:2804666/1‑148 (MQ=255)
                                                                                                                    aaGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTg                            <  1:2928002/148‑1 (MQ=255)
                                                                                                                     aGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGt                           <  2:306425/148‑1 (MQ=255)
                                                                                                                     aGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGt                           <  2:2119023/148‑1 (MQ=255)
                                                                                                                      gCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGtt                          >  2:922187/1‑148 (MQ=255)
                                                                                                                       cAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTa                         <  2:637709/148‑1 (MQ=255)
                                                                                                                          aTGAAAGCGTGTGATGATCTGGTTAAAGGTTTCGAAGATCTGTATACCAGCAAGTAATCTTTTTGGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTAccc                      <  2:1524648/148‑1 (MQ=255)
                                                                                                                          aTGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTAccc                      <  2:1950559/148‑1 (MQ=255)
                                                                                                                           tGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTAcccc                     <  2:1238573/148‑1 (MQ=255)
                                                                                                                           tGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTAcccc                     >  2:2791596/1‑148 (MQ=255)
                                                                                                                            gAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCt                    >  1:2147918/1‑148 (MQ=255)
                                                                                                                             aaaGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTg                   <  1:3057607/148‑1 (MQ=255)
                                                                                                                             aaaGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTg                   >  2:3368880/1‑148 (MQ=255)
                                                                                                                              aaGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGc                  >  2:1793713/1‑148 (MQ=255)
                                                                                                                                    ttGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCgg            >  1:1538786/1‑148 (MQ=255)
                                                                                                                                     tgatgtTCTGGTTAAAGTGTTCTAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGt           <  2:2111683/148‑1 (MQ=255)
                                                                                                                                     tgatgaTCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGt           <  2:2203744/148‑1 (MQ=255)
                                                                                                                                     tgatgaTCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGt           >  2:1217069/1‑148 (MQ=255)
                                                                                                                                       atgatCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGttt         >  2:1034619/1‑148 (MQ=255)
                                                                                                                                        tgatCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGTCCGCAGGCTGTTACCACAGAGGCCGGTTTc        >  1:3032821/1‑148 (MQ=255)
                                                                                                                                        tgatCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGAGACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTc        >  2:296640/1‑148 (MQ=255)
                                                                                                                                           tCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCggg     >  2:849729/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGC  >  CP009273/1264297‑1264586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCACGAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATT  >  CP009273/1264293‑1264591
                                                                                                                                                     |                                                                                                                                                     
GCACGAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTT                                                                                                                                                       >  SRR19973066.2275784/1‑150 (MQ=60)
GCACGAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTT                                                                                                                                                       >  SRR19973066.2307571/1‑150 (MQ=60)
 CACGAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTA                                                                                                                                                      >  SRR19973066.1752379/1‑150 (MQ=60)
   CGAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAA                                                                                                                                                    >  SRR19973066.308627/1‑150 (MQ=60)
    GAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAG                                                                                                                                                   <  SRR19973066.1081982/150‑1 (MQ=60)
    GAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAG                                                                                                                                                   <  SRR19973066.2941663/150‑1 (MQ=60)
    GAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAG                                                                                                                                                   <  SRR19973066.2941664/150‑1 (MQ=60)
    GAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGGTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAG                                                                                                                                                   <  SRR19973066.2941887/150‑1 (MQ=60)
     AGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGG                                                                                                                                                  <  SRR19973066.2107973/150‑1 (MQ=60)
     AGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGG                                                                                                                                                  >  SRR19973066.3254822/1‑150 (MQ=60)
         GGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTC                                                                                                                                              >  SRR19973066.2841386/1‑150 (MQ=60)
          GTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCG                                                                                                                                             <  SRR19973066.52071/150‑1 (MQ=60)
          GTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCG                                                                                                                                             >  SRR19973066.427767/1‑150 (MQ=60)
          GTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCG                                                                                                                                             <  SRR19973066.1082864/150‑1 (MQ=60)
             TGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGTGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAG                                                                                                                                          <  SRR19973066.893088/150‑1 (MQ=60)
              GGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGA                                                                                                                                         >  SRR19973066.642308/1‑150 (MQ=60)
                CGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAAC                                                                                                                                       <  SRR19973066.3284427/150‑1 (MQ=60)
                CGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAAC                                                                                                                                       <  SRR19973066.1621585/150‑1 (MQ=60)
                  GTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTG                                                                                                                                     >  SRR19973066.2189136/1‑150 (MQ=60)
                   TAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGG                                                                                                                                    <  SRR19973066.82912/150‑1 (MQ=60)
                      GTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATA                                                                                                                                 <  SRR19973066.413656/150‑1 (MQ=60)
                        CTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACC                                                                                                                               >  SRR19973066.641236/1‑150 (MQ=60)
                               GGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGT                                                                                                                        >  SRR19973066.1539316/1‑150 (MQ=60)
                                GCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTA                                                                                                                       <  SRR19973066.1179880/150‑1 (MQ=60)
                                  TGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAAT                                                                                                                     >  SRR19973066.2691588/1‑150 (MQ=60)
                                   GTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATC                                                                                                                    >  SRR19973066.2291131/1‑150 (MQ=60)
                                       ATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTGATCTTTT                                                                                                                <  SRR19973066.482155/150‑1 (MQ=60)
                                       ATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTT                                                                                                                >  SRR19973066.1122163/1‑150 (MQ=60)
                                        TTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTT                                                                                                               <  SRR19973066.926906/150‑1 (MQ=60)
                                          GAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTG                                                                                                             <  SRR19973066.3156990/150‑1 (MQ=60)
                                           AAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGC                                                                                                            >  SRR19973066.1122368/1‑150 (MQ=60)
                                            AGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCT                                                                                                           <  SRR19973066.2397586/150‑1 (MQ=60)
                                             GCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTT                                                                                                          <  SRR19973066.163660/150‑1 (MQ=60)
                                                   CCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAA                                                                                                    >  SRR19973066.1996237/1‑150 (MQ=60)
                                                   CCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAA                                                                                                    >  SRR19973066.1471887/1‑150 (MQ=60)
                                                   CCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAA                                                                                                    >  SRR19973066.1471852/1‑150 (MQ=60)
                                                     GGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATA                                                                                                  <  SRR19973066.40866/150‑1 (MQ=60)
                                                     GGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATA                                                                                                  >  SRR19973066.3230345/1‑150 (MQ=60)
                                                      GATCCTGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAA                                                                                                 >  SRR19973066.1924080/1‑150 (MQ=60)
                                                       ATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAA                                                                                                <  SRR19973066.1496141/150‑1 (MQ=60)
                                                       ATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAA                                                                                                <  SRR19973066.1495542/150‑1 (MQ=60)
                                                          CGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTA                                                                                             >  SRR19973066.2382871/1‑150 (MQ=60)
                                                          CGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTA                                                                                             >  SRR19973066.2382881/1‑150 (MQ=60)
                                                           GGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTAT                                                                                            <  SRR19973066.3099267/150‑1 (MQ=60)
                                                             AACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTA                                                                                          <  SRR19973066.2556750/150‑1 (MQ=60)
                                                              ACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAG                                                                                         <  SRR19973066.249115/150‑1 (MQ=60)
                                                                ATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCG                                                                                       >  SRR19973066.157255/1‑150 (MQ=60)
                                                                ATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCG                                                                                       >  SRR19973066.1974882/1‑150 (MQ=60)
                                                                ATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCG                                                                                       >  SRR19973066.3362497/1‑150 (MQ=60)
                                                                ATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCG                                                                                       >  SRR19973066.3362413/1‑150 (MQ=60)
                                                                 TGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGT                                                                                      <  SRR19973066.1716226/150‑1 (MQ=60)
                                                                  GCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTT                                                                                     <  SRR19973066.3538567/150‑1 (MQ=60)
                                                                  GCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTT                                                                                     <  SRR19973066.3538541/150‑1 (MQ=60)
                                                                     AAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAATATAAAGTATTAGCGTTCTG                                                                                  >  SRR19973066.2486559/1‑150 (MQ=60)
                                                                     AAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAATGTATTAGCGTTCTG                                                                                  >  SRR19973066.1719369/1‑150 (MQ=60)
                                                                     AAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTG                                                                                  >  SRR19973066.1719380/1‑150 (MQ=60)
                                                                     AAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTG                                                                                  <  SRR19973066.1777720/150‑1 (MQ=60)
                                                                       ATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCG                                                                                >  SRR19973066.2219012/1‑150 (MQ=60)
                                                                       ATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCG                                                                                >  SRR19973066.2219017/1‑150 (MQ=60)
                                                                       ATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCG                                                                                <  SRR19973066.596951/150‑1 (MQ=60)
                                                                           GATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAA                                                                            >  SRR19973066.3167586/1‑150 (MQ=60)
                                                                           GATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAA                                                                            >  SRR19973066.3167804/1‑150 (MQ=60)
                                                                            ATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAG                                                                           <  SRR19973066.2604587/150‑1 (MQ=60)
                                                                             TGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGA                                                                          <  SRR19973066.2780714/150‑1 (MQ=60)
                                                                               GTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACT                                                                        >  SRR19973066.301728/1‑150 (MQ=60)
                                                                                TCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTT                                                                       <  SRR19973066.3218828/150‑1 (MQ=60)
                                                                                TCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTT                                                                       <  SRR19973066.1853478/150‑1 (MQ=60)
                                                                                   ATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTT                                                                    <  SRR19973066.2810069/150‑1 (MQ=60)
                                                                                   ATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTT                                                                    <  SRR19973066.3319162/150‑1 (MQ=60)
                                                                                   ATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTT                                                                    >  SRR19973066.65263/1‑150 (MQ=60)
                                                                                    TCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTT                                                                   <  SRR19973066.1429662/150‑1 (MQ=60)
                                                                                    TCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTAGTAGCGTTCTGCGTTAAGACTTTTTT                                                                   <  SRR19973066.809085/150‑1 (MQ=60)
                                                                                      CGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCA                                                                 <  SRR19973066.3380274/150‑1 (MQ=60)
                                                                                          CTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGG                                                             <  SRR19973066.1637420/150‑1 (MQ=60)
                                                                                           TGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCTTGTAATCTTTTTTGATTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGT                                                            <  SRR19973066.3521303/150‑1 (MQ=60)
                                                                                           TGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGT                                                            >  SRR19973066.1337935/1‑150 (MQ=60)
                                                                                                CTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGG                                                       >  SRR19973066.509765/1‑150 (MQ=60)
                                                                                                  GGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATC‑TTTTTGTTTGAAAAATAAAGTCTTAGCGTTGTGCGTTAAGACTTTTTT‑ATGGGTGCCGGATAC                                                   >  SRR19973066.1021790/1‑150 (MQ=60)
                                                                                                     TAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACA                                                  <  SRR19973066.477517/150‑1 (MQ=60)
                                                                                                      AAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAA                                                 >  SRR19973066.1703992/1‑150 (MQ=60)
                                                                                                        ACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAA                                               <  SRR19973066.2703696/150‑1 (MQ=60)
                                                                                                          TGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAA                                             >  SRR19973066.3293611/1‑150 (MQ=60)
                                                                                                          TGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATATGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAA                                             <  SRR19973066.3531871/150‑1 (MQ=60)
                                                                                                             AACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGC                                          >  SRR19973066.789008/1‑150 (MQ=60)
                                                                                                               CCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCG                                        <  SRR19973066.70019/150‑1 (MQ=60)
                                                                                                                CGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGTACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGC                                       >  SRR19973066.1642686/1‑150 (MQ=60)
                                                                                                                 GTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCA                                      >  SRR19973066.2075605/1‑150 (MQ=60)
                                                                                                                     CTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAATAAAGGCCGCAGGCT                                  >  SRR19973066.3234669/1‑150 (MQ=60)
                                                                                                                     CTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCT                                  >  SRR19973066.3234393/1‑150 (MQ=60)
                                                                                                                       CAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGT                                >  SRR19973066.1412560/1‑150 (MQ=60)
                                                                                                                        aagcattttAAAGCGATTGATGATCTGTTTAAAGGTTTCGAAGATCTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTGGTCGTTAAGACTTTTTTCTTGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTT                               <  SRR19973066.1158064/141‑1 (MQ=60)
                                                                                                                        AAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTT                               >  SRR19973066.1568435/1‑150 (MQ=60)
                                                                                                                        AAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTT                               <  SRR19973066.2946926/150‑1 (MQ=60)
                                                                                                                        AAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTT                               <  SRR19973066.2947501/150‑1 (MQ=60)
                                                                                                                              ATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCT                         >  SRR19973066.2162825/1‑150 (MQ=60)
                                                                                                                                 AAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCG                      <  SRR19973066.3078055/150‑1 (MQ=60)
                                                                                                                                  AAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGG                     >  SRR19973066.2304988/1‑150 (MQ=60)
                                                                                                                                      GATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGG                 >  SRR19973066.1549773/1‑150 (MQ=60)
                                                                                                                                      GATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGG                 >  SRR19973066.1549764/1‑150 (MQ=60)
                                                                                                                                          GATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGTCCGCAGGCTGTTACCACAGAGGCCGGTTTC             >  SRR19973066.3053084/1‑150 (MQ=60)
                                                                                                                                          GATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTC             >  SRR19973066.3053196/1‑150 (MQ=60)
                                                                                                                                          GATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAATACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTC             >  SRR19973066.2575016/1‑150 (MQ=60)
                                                                                                                                                CTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGC       >  SRR19973066.1484180/1‑150 (MQ=60)
                                                                                                                                                   GTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCCTA    >  SRR19973066.1928904/1‑150 (MQ=60)
                                                                                                                                                   GTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATA    >  SRR19973066.437371/1‑150 (MQ=60)
                                                                                                                                                   GTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATA    >  SRR19973066.1928921/1‑150 (MQ=60)
                                                                                                                                                     TAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATT  <  SRR19973066.2841195/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GCACGAGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATT  >  CP009273/1264293‑1264591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑