Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 163,686 C→A 38.5% Q824K (CAG→AAG)  mrcB → fused glycosyl transferase and transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273163,6860CA38.5% 356.6 / 276.8 261Q824K (CAG→AAG) mrcBfused glycosyl transferase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base C (81/79);  new base A (31/70);  total (112/149)
Fisher's exact test for biased strand distribution p-value = 2.00e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 261 total aligned reads displayed.

TGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCCTTTCTTTTCAT  >  CP009273/163538‑163833
                                                                                                                                                     |                                                                                                                                                   
    tgCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCaa                                                                                                                                                >  1:1376592/1‑150 (MQ=255)
     gCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAag                                                                                                                                               <  1:4324433/150‑1 (MQ=255)
          ggTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAg                                                                                                                                          <  2:3988545/150‑1 (MQ=255)
              gCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAg                                                                                                                                      >  2:2876848/1‑150 (MQ=255)
               cATGCGTATCTTGCCGGTCTGGTCCAGCGATCCGCAATCGCTGTGCTAGCAGAGCGAGATGCAGCTGCAGCCGTGAGTCAATCCGTTTGATAAGT‑CTTCTGAGCCGCAGCAACATCCGCAACAGCATCCTGCTAATCAAGAGCAGATAGa                                                                                                                                     <  2:3586945/150‑1 (MQ=255)
                  gCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAg                                                                                                                                  <  1:4427535/150‑1 (MQ=255)
                    gTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCg                                                                                                                                <  2:12450/150‑1 (MQ=255)
                      aTCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGAc                                                                                                                              <  1:2311642/150‑1 (MQ=255)
                      aTCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGAc                                                                                                                              >  1:1240149/1‑150 (MQ=255)
                        cTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACgg                                                                                                                            <  1:4453856/150‑1 (MQ=255)
                        cTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACgg                                                                                                                            >  2:3295182/1‑150 (MQ=255)
                         ttGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGgt                                                                                                                           >  2:4524013/1‑150 (MQ=255)
                         ttGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGgt                                                                                                                           <  1:3839085/150‑1 (MQ=255)
                          tGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGgtg                                                                                                                          >  1:4389833/1‑150 (MQ=255)
                              ggTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGc                                                                                                                      >  2:1757073/1‑150 (MQ=255)
                                 cTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCgg                                                                                                                   >  2:9055/1‑150 (MQ=255)
                                 cTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCgg                                                                                                                   >  2:2317455/1‑150 (MQ=255)
                                    gACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTg                                                                                                                >  2:367639/1‑150 (MQ=255)
                                     aCCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTgg                                                                                                               >  1:1252398/1‑150 (MQ=255)
                                       cAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGAt                                                                                                             <  1:3794765/150‑1 (MQ=255)
                                       cAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGAt                                                                                                             >  1:2769932/1‑150 (MQ=255)
                                       cAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGAt                                                                                                             >  1:3103392/1‑150 (MQ=255)
                                         gCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCa                                                                                                           <  2:108649/150‑1 (MQ=255)
                                         gCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCa                                                                                                           <  2:3778048/150‑1 (MQ=255)
                                          cGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCaa                                                                                                          <  1:3050056/150‑1 (MQ=255)
                                           gATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAg                                                                                                         >  1:3404680/1‑150 (MQ=255)
                                           gATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAg                                                                                                         >  1:2840090/1‑150 (MQ=255)
                                              ccGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGat                                                                                                      >  1:925397/1‑150 (MQ=255)
                                               cGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGata                                                                                                     <  2:2608359/150‑1 (MQ=255)
                                                gCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGatat                                                                                                    <  1:1484440/150‑1 (MQ=255)
                                                  aaTCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCCGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGt                                                                                                  >  2:4059756/1‑150 (MQ=255)
                                                  aaTCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGt                                                                                                  <  2:4449303/150‑1 (MQ=255)
                                                   aTCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATggt                                                                                                 >  2:3374620/1‑148 (MQ=255)
                                                    tCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGttt                                                                                                <  1:34301/150‑1 (MQ=255)
                                                     cGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTg                                                                                               <  1:4393501/150‑1 (MQ=255)
                                                      gCTGTGCCAACAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTgg                                                                                              <  2:1289560/150‑1 (MQ=255)
                                                        tgtgCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGgta                                                                                            >  2:4386685/1‑150 (MQ=255)
                                                            ccAGCTGAGCTAGTTGCAGCAGCTGCCTTCTGCAAATCCTTTTTA‑CATTACTACACAGCCGACGAAACCCCGGAAACAGAAACCGGCTCACAAATCGAATAACGCCAGC‑ACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAAt                                                                                       <  2:558230/150‑1 (MQ=255)
                                                             cagcagAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAAt                                                                                       >  1:250834/1‑150 (MQ=255)
                                                              agcagAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAAtt                                                                                      <  1:3577902/150‑1 (MQ=255)
                                                              agcagAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAAtt                                                                                      <  1:1349626/150‑1 (MQ=255)
                                                                cagAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTaa                                                                                    >  1:115507/1‑150 (MQ=255)
                                                                 agagCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAAc                                                                                   >  2:4205843/1‑150 (MQ=255)
                                                                  gagCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACa                                                                                  >  1:1978088/1‑150 (MQ=255)
                                                                   agCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACAt                                                                                 >  1:2943962/1‑150 (MQ=255)
                                                                      gagaTGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTa                                                                              >  1:1803370/1‑150 (MQ=255)
                                                                      gagaTGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTa                                                                              >  2:1408240/1‑150 (MQ=255)
                                                                           gcagcagcaGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGt                                                                         >  1:339612/1‑150 (MQ=255)
                                                                                 gcagcCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATa                                                                   <  1:2979934/150‑1 (MQ=255)
                                                                                     ccGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCgg                                                               <  1:455443/150‑1 (MQ=255)
                                                                                       gTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGAt                                                             <  2:3096712/150‑1 (MQ=255)
                                                                                        tCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATg                                                            <  2:10225/150‑1 (MQ=255)
                                                                                         cAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATgg                                                           >  2:3479813/1‑150 (MQ=255)
                                                                                         cAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATgg                                                           >  2:1538894/1‑150 (MQ=255)
                                                                                           ggCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCg                                                         >  2:3736249/1‑150 (MQ=255)
                                                                                           ggCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCg                                                         <  2:2101513/150‑1 (MQ=255)
                                                                                            gCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGa                                                        <  2:4348373/150‑1 (MQ=255)
                                                                                             cAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAg                                                       >  2:1329355/1‑150 (MQ=255)
                                                                                              aaTCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGt                                                      <  2:1376592/150‑1 (MQ=255)
                                                                                                tCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTg                                                    >  2:3235751/1‑150 (MQ=255)
                                                                                                tCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTg                                                    <  1:4554956/150‑1 (MQ=255)
                                                                                                 ccGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGc                                                   >  1:2664896/1‑150 (MQ=255)
                                                                                                  cGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGcc                                                  <  2:3254439/150‑1 (MQ=255)
                                                                                                  cGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGcc                                                  <  2:2732821/150‑1 (MQ=255)
                                                                                                      tGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATcc                                              >  2:369568/1‑150 (MQ=255)
                                                                                                       gATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCg                                             <  2:2042149/150‑1 (MQ=255)
                                                                                                         tCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGt                                           <  2:699792/150‑1 (MQ=255)
                                                                                                           aGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTaa                                         >  2:4591250/1‑150 (MQ=255)
                                                                                                            gT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTaaa                                        <  1:3632732/150‑1 (MQ=255)
                                                                                                                   tcAGCAGCAGCAACATCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTATCCGGTTGGATCACGGATATGGTTGGTAGTAATTAACATCTAAGCTTGAAATACCGGAAGGCGAGTTGCCATCCGGTAAAATAACa                                  >  1:2108938/1‑150 (MQ=255)
                                                                                                                     aGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATc                                <  1:2681248/150‑1 (MQ=255)
                                                                                                                     aGCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATc                                <  2:4081728/150‑1 (MQ=255)
                                                                                                                      gCCGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAACATAACATcc                               <  1:693390/150‑1 (MQ=255)
                                                                                                                       ccGCAGCAACAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATccc                              <  1:1474227/150‑1 (MQ=255)
                                                                                                                        cGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCa                             >  1:763896/1‑150 (MQ=255)
                                                                                                                           agcaACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCt                          <  2:1928071/150‑1 (MQ=255)
                                                                                                                           agcaACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCt                          >  2:2455909/1‑150 (MQ=255)
                                                                                                                            gcaACCGCCGCCACCGCAACCTGCTAAGCAAGCGCAGAACGACAGCGCCGGGGTAGCCGGGTGGATCAAGGCTATGTTTGGTAGTAATTAACATCTACGCGTGAAATACCGGCTGGCGAGTTGCCCTCCGGTAACATAACATCCCATCTa                         <  2:3104416/150‑1 (MQ=255)
                                                                                                                             caACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTaa                        <  1:4494537/150‑1 (MQ=255)
                                                                                                                              aaCAGCCGCAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAg                       <  2:3039120/150‑1 (MQ=255)
                                                                                                                                  gCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGatat                   <  2:1616410/150‑1 (MQ=255)
                                                                                                                                    cGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTa                 <  1:316204/150‑1 (MQ=255)
                                                                                                                                      cAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAAc               <  1:2376076/150‑1 (MQ=255)
                                                                                                                                      cAACAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAAc               >  1:398575/1‑150 (MQ=255)
                                                                                                                                       aaCAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAAcc              >  1:645953/1‑150 (MQ=255)
                                                                                                                                       aaCAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAAcc              >  1:644159/1‑150 (MQ=255)
                                                                                                                                       aaCAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAAcc              >  2:3809559/1‑150 (MQ=255)
                                                                                                                                       aaCAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAAcc              >  2:1943315/1‑150 (MQ=255)
                                                                                                                                        aCAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATGAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAAccc             <  2:1071904/150‑1 (MQ=255)
                                                                                                                                         cAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCct            <  2:3946675/150‑1 (MQ=255)
                                                                                                                                         cAGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCct            <  1:4236876/150‑1 (MQ=255)
                                                                                                                                          aGCAACCTGCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGTATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCctt           <  1:3675889/150‑1 (MQ=255)
                                                                                                                                           gCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCcttt          <  1:4205843/150‑1 (MQ=255)
                                                                                                                                           gCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCcttt          >  1:3769198/1‑150 (MQ=255)
                                                                                                                                             aaCCTGCACAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCctttct        <  1:1578008/150‑1 (MQ=255)
                                                                                                                                              aCCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCctttctt       >  1:1416479/1‑150 (MQ=255)
                                                                                                                                                cggCTAAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCCTTTCtttt     <  1:324191/148‑1 (MQ=255)
                                                                                                                                                cTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCCTTTCtttt     <  1:1035473/150‑1 (MQ=255)
                                                                                                                                                   cTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCCTTTCTTTTCAt  >  1:1909475/1‑150 (MQ=255)
                                                                                                                                                   cTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCCTTTCTTTTCAt  >  1:3734968/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAGCAGCAGCCGTCAGGCAATCCGTTTGATCAGT‑CTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCCTTTCTTTTCAT  >  CP009273/163538‑163833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A