Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 360,317 C→A 42.9% intergenic (‑251/+344) lacZ ← / ← lacI pseudogene, truncated/DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273360,3170CA42.9% 208.7 / 229.6 199intergenic (‑251/+344)lacZ/lacIpseudogene, truncated/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (79/34);  new base A (54/32);  total (133/66)
Fisher's exact test for biased strand distribution p-value = 3.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 202 total aligned reads displayed.

CAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAGCCCGTAGTCCCGT  >  CP009273/360168‑360466
                                                                                                                                                     |                                                                                                                                                     
cAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTc                                                                                                                                                       <  1:1902294/150‑1 (MQ=255)
cAAATCCGCTCCCGGCGGATTGGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTc                                                                                                                                                       <  1:1390573/150‑1 (MQ=255)
 aactCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCg                                                                                                                                                      <  1:4290083/147‑1 (MQ=255)
  aaTCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGt                                                                                                                                                     >  1:3662322/1‑150 (MQ=255)
       gCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATAc                                                                                                                                                <  1:1127592/150‑1 (MQ=255)
         tCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGc                                                                                                                                              >  2:1099062/1‑150 (MQ=255)
          cccGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTG‑‑‑‑‑‑‑GGGAATTAATTCCTGAAGACGAAAGGGCCTAGTGATACGCCTAtgggt                                                                                                                                      >  2:2916555/1‑146 (MQ=14)
             ggcggATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTAt                                                                                                                                          >  2:4411661/1‑150 (MQ=255)
                ggATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAACCGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTAtttt                                                                                                                                       <  2:3525553/150‑1 (MQ=255)
                ggATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTAtttt                                                                                                                                       <  2:3252982/150‑1 (MQ=255)
                ggATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTAgttt                                                                                                                                       >  1:3382681/1‑150 (MQ=255)
                ggATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTAtttt                                                                                                                                       >  1:3421964/1‑150 (MQ=255)
                 gATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTAttttt                                                                                                                                      >  1:395561/1‑150 (MQ=255)
                     tGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAg                                                                                                                                  >  1:732821/1‑150 (MQ=255)
                     tGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAg                                                                                                                                  >  1:2757059/1‑150 (MQ=255)
                      gTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAgg                                                                                                                                 >  2:3943442/1‑150 (MQ=255)
                      gTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAgg                                                                                                                                 >  2:3991373/1‑150 (MQ=255)
                        ccTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGtt                                                                                                                               <  2:2788220/150‑1 (MQ=255)
                         cgaCTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTa                                                                                                                              <  1:3033371/148‑1 (MQ=255)
                                 ttgagCTTTCACCGAAAAATAACCGATTAAAAGTAATGCCCAGTCTTTCGAATGATAACTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTTTTTTTATAGGTTAATGTCATg                                                                                                                      <  1:1921262/148‑1 (MQ=25)
                                 gagaGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATg                                                                                                                      >  1:405368/1‑150 (MQ=255)
                                     gCGTTAACCGACAAACACCAGATAAAACGATAGGCCAATTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAATGGGCCTCGTGATACGCCTTTTTTTATAGGTTAATGTCATGGGac                                                                                                                  <  1:2964833/150‑2 (MQ=25)
                                      cGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTACGACTGAGCCTTTCGTGTTATTTGATGCCTGGGGAATTGGTAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTAGTGTTAGAGGTTCATGTCATGGGAAg                                                                                                                 >  2:2755784/1‑150 (MQ=25)
                                       gTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGACTTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGa                                                                                                                >  2:213738/1‑150 (MQ=255)
                                        ttCACCGACCACCAACATATAAAACGAAAGGCCCAGTCTCACGACTGAGCCTTTCGTTTGATTTGACGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGac                                                                                                               <  1:4466920/150‑2 (MQ=37)
                                        ttCACCGACAAACAACAGATAAAACGAATGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAAGTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTGATAGGTTAATGTCATGGGAAGAt                                                                                                               >  1:3046256/1‑150 (MQ=255)
                                          cACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATcc                                                                                                             >  1:328846/1‑150 (MQ=255)
                                            ccGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAg                                                                                                           <  1:1165507/150‑1 (MQ=255)
                                                  cacaacaGCTAACTCCCAAGGCCCCGTCTTTGGACTGAGCCTTTCTTTTTTTTTGCTGCCTG‑‑‑‑‑‑‑GGGAATTAATTCCTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCttt                                                                                              <  1:2916555/149‑1 (MQ=255)
                                                   acaacaGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCa                                                                                                    <  2:3441584/150‑1 (MQ=255)
                                                   acaacaGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAACTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCa                                                                                                    <  2:4197361/150‑1 (MQ=255)
                                                     aacaGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGTTGCCTGGGGACTTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAgg                                                                                                  >  2:4220300/1‑150 (MQ=255)
                                                     aacaGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAgg                                                                                                  >  1:3828461/1‑150 (MQ=255)
                                                        aGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCtt                                                                                               >  1:4628030/1‑150 (MQ=255)
                                                        aGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCtt                                                                                               >  1:2298869/1‑150 (MQ=255)
                                                            aaaaCGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCa                                                                                           >  1:1267683/1‑150 (MQ=255)
                                                             aaaCGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTGATTTGATGCCGGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCGTTCCAc                                                                                          >  2:3246329/1‑150 (MQ=255)
                                                              aaCGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACg                                                                                         >  1:3173051/1‑150 (MQ=255)
                                                                  aaaGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCcg                                                                                     >  1:4294100/1‑150 (MQ=255)
                                                                    aGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCcgcg                                                                                   >  1:1609277/1‑150 (MQ=255)
                                                                      gCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGACTTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTg                                                                                 >  2:1461513/1‑150 (MQ=255)
                                                                        ccAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGcc                                                                               >  1:2636020/1‑150 (MQ=255)
                                                                        ccAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAAGTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGcc                                                                               >  1:322990/1‑150 (MQ=255)
                                                                         cAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCg                                                                              >  2:2589950/1‑150 (MQ=255)
                                                                          aGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCa                                                                                                    <  1:776095/127‑1 (MQ=255)
                                                                          aGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGc                                                                             >  1:274876/1‑150 (MQ=255)
                                                                                tCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATg                                                                       >  1:1769071/1‑150 (MQ=255)
                                                                                tCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATg                                                                       >  1:4470183/1‑150 (MQ=255)
                                                                                tCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATg                                                                       >  1:4094727/1‑150 (MQ=255)
                                                                                     tGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGtt                                                                  <  1:3772935/150‑1 (MQ=255)
                                                                                      gAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTc                                                                 <  2:3240347/150‑1 (MQ=255)
                                                                                       aGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTcg                                                                >  2:4220715/1‑150 (MQ=255)
                                                                                       aGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTcg                                                                <  1:156720/150‑1 (MQ=255)
                                                                                         ccTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTcgcg                                                              >  2:4472707/1‑150 (MQ=255)
                                                                                           tttCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTcgcgcg                                                            >  2:2325077/1‑150 (MQ=255)
                                                                                               gTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTtc                                                        <  2:1620756/150‑1 (MQ=255)
                                                                                                 tttATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTtctc                                                      >  2:1460699/1‑150 (MQ=255)
                                                                                                   tATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCgg                                                    >  2:1223121/1‑150 (MQ=255)
                                                                                                     tttGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAAGGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGaa                                                  <  1:4401009/150‑1 (MQ=255)
                                                                                                      ttGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGaaa                                                 <  2:3292080/150‑1 (MQ=255)
                                                                                                       tGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAAc                                                >  1:3231409/1‑150 (MQ=255)
                                                                                                       tGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAAc                                                <  2:1707428/150‑1 (MQ=255)
                                                                                                       tGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAAc                                                <  2:1311669/150‑1 (MQ=255)
                                                                                                        gATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAAcg                                               >  2:104540/1‑150 (MQ=255)
                                                                                                         aTGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAAcgc                                              >  1:2521299/1‑150 (MQ=255)
                                                                                                            ccTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCt                                           <  1:2637387/150‑1 (MQ=255)
                                                                                                             cTGGGGAATTGGGAATTCATTCTTGAAGACGAACGGGCCTAGTGATACGCCTATTTTGATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCGCGGAAACGCGCTg                                          <  1:3675374/150‑1 (MQ=255)
                                                                                                             cTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTg                                          >  2:1507790/1‑150 (MQ=255)
                                                                                                               ggggAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGcc                                        >  1:1964868/1‑150 (MQ=255)
                                                                                                                gggaattgggaattAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCcg                                       <  1:811314/150‑1 (MQ=255)
                                                                                                                gggaattgggaattAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCcg                                       >  2:3817224/1‑150 (MQ=255)
                                                                                                                gggaattgggaattAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCcg                                       >  1:4190497/1‑150 (MQ=255)
                                                                                                                 ggaattgggaattAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCcgc                                      >  1:653946/1‑150 (MQ=255)
                                                                                                                 ggaattgggaattAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCcgc                                      >  1:1998492/1‑150 (MQ=255)
                                                                                                                      tgggaattAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTc                                 <  2:155548/150‑1 (MQ=255)
                                                                                                                      tgggaattAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTc                                 <  1:4027127/150‑1 (MQ=255)
                                                                                                                      tgggaattAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTc                                 >  2:3704388/1‑150 (MQ=255)
                                                                                                                      tgggaattAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTGAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTc                                 <  2:2753921/150‑1 (MQ=255)
                                                                                                                        ggaattAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGt                               >  2:3126936/1‑150 (MQ=255)
                                                                                                                          aattaattCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGa                             >  2:251233/1‑150 (MQ=255)
                                                                                                                           attaattCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGAt                            >  2:1550685/1‑150 (MQ=255)
                                                                                                                                 tCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCAc                      >  1:2464125/1‑150 (MQ=255)
                                                                                                                                  cTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACg                     >  2:1319704/1‑150 (MQ=255)
                                                                                                                                  cTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACg                     <  1:1099062/150‑1 (MQ=255)
                                                                                                                                  cTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACg                     >  1:1271378/1‑150 (MQ=255)
                                                                                                                                   ttGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGc                    >  2:3266527/1‑150 (MQ=255)
                                                                                                                                    tGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCt                   >  2:143342/1‑150 (MQ=255)
                                                                                                                                     gAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTTATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTc                  <  1:4049476/150‑1 (MQ=255)
                                                                                                                                     gAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTc                  >  2:3206140/1‑150 (MQ=255)
                                                                                                                                     gAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTc                  >  2:3205927/1‑150 (MQ=255)
                                                                                                                                      aaGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCa                 >  2:1642835/1‑150 (MQ=255)
                                                                                                                                      aaGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCa                 >  1:3574277/1‑150 (MQ=255)
                                                                                                                                        gACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGGTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAg               <  1:3130783/150‑1 (MQ=255)
                                                                                                                                        gACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAg               <  2:1739433/150‑1 (MQ=255)
                                                                                                                                         aCGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAGc              >  1:1468394/1‑150 (MQ=255)
                                                                                                                                          cGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAGcc             <  1:2331209/150‑1 (MQ=255)
                                                                                                                                             aaGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAGCCCGt          >  2:1559957/1‑150 (MQ=255)
                                                                                                                                              aGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAGCCCGTa         >  1:1247252/1‑150 (MQ=255)
                                                                                                                                                 gCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAGCCCGTAGTc      >  2:4355724/1‑150 (MQ=255)
                                                                                                                                                     cGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAGCCCGTAGTCCCGt  >  2:2052707/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAGCCCGTAGTCCCGT  >  CP009273/360168‑360466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A