Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 360,317 C→A 48.2% intergenic (‑251/+344) lacZ ← / ← lacI pseudogene, truncated/DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273360,3170CA48.2% 118.8 / 288.9 205intergenic (‑251/+344)lacZ/lacIpseudogene, truncated/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (77/29);  new base A (73/26);  total (150/55)
Fisher's exact test for biased strand distribution p-value = 8.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

CAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAGCCCGTAGTCCCGT  >  CP009273/360168‑360466
                                                                                                                                                     |                                                                                                                                                     
       gCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATAc                                                                                                                                                <  1:1497394/150‑1 (MQ=255)
         tCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGc                                                                                                                                              >  1:3541431/1‑150 (MQ=255)
         tCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGc                                                                                                                                              >  1:1228190/1‑150 (MQ=255)
         tCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAAGTAATTCTTGAAGACGAAAGGGCCTCGTGATACGc                                                                                                                                              >  1:898619/1‑150 (MQ=255)
            cggcggATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTa                                                                                                                                           >  1:1066238/1‑150 (MQ=255)
             ggcggATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTAt                                                                                                                                          <  1:658003/150‑1 (MQ=255)
             ggcggATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTAt                                                                                                                                          <  2:1228646/150‑1 (MQ=255)
              gcggATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTAtt                                                                                                                                         <  2:4140903/150‑1 (MQ=255)
                ggATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTAtttt                                                                                                                                       >  2:276528/1‑150 (MQ=255)
                 gATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGACTTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTAttttt                                                                                                                                      >  1:2503025/1‑150 (MQ=255)
                 gATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGAGGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTAttttt                                                                                                                                      <  1:3715256/150‑1 (MQ=255)
                  aTTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTta                                                                                                                                     >  2:2552703/1‑150 (MQ=255)
                      gTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAgg                                                                                                                                 >  1:1851093/1‑150 (MQ=255)
                      gTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAgg                                                                                                                                 >  1:3441721/1‑150 (MQ=255)
                      gTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAgg                                                                                                                                 >  2:662560/1‑150 (MQ=255)
                        ccTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTGTTAGTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGtt                                                                                                                               >  2:3464269/1‑150 (MQ=255)
                        ccTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTGTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGtt                                                                                                                               <  1:1166957/150‑1 (MQ=255)
                            cTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATg                                                                                                                           >  2:254073/1‑150 (MQ=255)
                               aGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGGTAATGTCa                                                                                                                        >  2:1554672/1‑150 (MQ=255)
                               aGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCa                                                                                                                        >  2:3647449/1‑150 (MQ=255)
                                ggAGAGCGTTCACCGACAAACAACAGATAAATCGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGTCCTAGTGATACGCCTATTTTTATTGGTGAATGTCAt                                                                                                                       >  2:2777471/1‑150 (MQ=25)
                                 gagaGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATg                                                                                                                      >  2:2058639/1‑150 (MQ=255)
                                  agagCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATTCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTCTTTTTATAGGTGAATGTCATgg                                                                                                                     >  2:1628759/1‑150 (MQ=255)
                                   gagCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGACTTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATggg                                                                                                                    >  1:2706928/1‑150 (MQ=255)
                                     gCGGTCACCGACAACCCCCACATAACACGAAACGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGaa                                                                                                                  <  2:2008176/150‑1 (MQ=37)
                                      cGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAg                                                                                                                 >  2:2101734/1‑150 (MQ=255)
                                        ttCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGACTTGGGCATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGAt                                                                                                               >  2:3341894/1‑150 (MQ=255)
                                          cACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATcc                                                                                                             <  1:2946480/150‑1 (MQ=255)
                                           aCCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCa                                                                                                            >  1:802180/1‑150 (MQ=255)
                                              gACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTGTATAGGTTAAGGTCATGGGACGATCCAGcc                                                                                                         >  2:529977/1‑150 (MQ=255)
                                               acaaacaaCAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGACTTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCGATTTTTAGAGGGTAAGGTCATGGGAAGATCCAGCCg                                                                                                        >  2:4122528/1‑150 (MQ=25)
                                                 aaacaaCAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCGGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTCTTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGAc                                                                                                      >  2:3492732/1‑150 (MQ=255)
                                                 aaacaaCAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCATTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGAc                                                                                                      >  2:793491/1‑150 (MQ=255)
                                                  aacaacAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTGTTATAGGTTAATGTCATGGGAAGATCCAGCCGAcc                                                                                                     >  2:4054656/1‑150 (MQ=255)
                                                  aacaacAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGAcc                                                                                                     >  1:3110708/1‑150 (MQ=255)
                                                  aacaacAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTGTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGACGATCCAGCCGAcc                                                                                                     >  2:829008/1‑150 (MQ=255)
                                                    caacaGATAATACGAAAGGCCCAGTCTTTCGACTGAGCCTTTGGTTTTATTTGATGCGTGGGGAATTGGGAATTAATTGTTGATGACGAAAGGGCCTCGGGATACGCCTATTGGTATTGGGTCATGGGATGGGAAGATCCAGCCGACCAg                                                                                                   >  2:4006101/1‑150 (MQ=25)
                                                    caacaGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAg                                                                                                   >  1:4048716/1‑150 (MQ=255)
                                                         gATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTGTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCttt                                                                                              >  1:2286170/1‑150 (MQ=255)
                                                          aTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTc                                                                                             >  1:1064121/1‑150 (MQ=255)
                                                          aTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGAGACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTc                                                                                             >  1:3761370/1‑150 (MQ=255)
                                                          aTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTc                                                                                             <  1:2087968/150‑1 (MQ=255)
                                                           tAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTcc                                                                                            >  1:2330279/1‑150 (MQ=255)
                                                              aaCGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACg                                                                                         <  1:2691369/150‑1 (MQ=255)
                                                              aaCGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTGTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACg                                                                                         >  2:430615/1‑150 (MQ=255)
                                                               aCGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATGGTTCTAGGTTAATGTCAGGGGAAGATCCAGCCGACCAGGCTTTCCACGc                                                                                        >  1:3499585/1‑150 (MQ=255)
                                                                  atcggCCCAGTCTTTCGACAGAGCCTTTCGATTTATTTGATGCCTGGTGAATTTGGAATAAATTCTTGAAGTCGACAGTGCCTAGTGATACGCCTATTTTTATAGGTTTATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCcg                                                                                     <  1:3874736/147‑1 (MQ=255)
                                                                  aaaGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCcg                                                                                     <  2:3903573/150‑1 (MQ=255)
                                                                  aaaGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCcg                                                                                     >  1:1929655/1‑150 (MQ=255)
                                                                    aGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCcgcg                                                                                   >  1:985872/1‑150 (MQ=255)
                                                                       cccAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGc                                                                                >  1:3579135/1‑150 (MQ=255)
                                                                             cTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTcc                                                                          >  1:3447202/1‑150 (MQ=255)
                                                                                tCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATg                                                                       >  1:2994517/1‑150 (MQ=255)
                                                                                 cGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGt                                                                      >  1:594333/1‑150 (MQ=255)
                                                                                 cGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGt                                                                      >  1:2774723/1‑150 (MQ=255)
                                                                                   aCTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCg                                                                    <  2:465216/150‑1 (MQ=255)
                                                                                    cTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGt                                                                   >  1:4178062/1‑150 (MQ=255)
                                                                                      gAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTc                                                                 <  1:1700613/150‑1 (MQ=255)
                                                                                      gAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTc                                                                 <  1:2731643/150‑1 (MQ=255)
                                                                                       aGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTcg                                                                >  1:2783854/1‑150 (MQ=255)
                                                                                           tttCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTcgcgcg                                                            >  2:1865881/1‑150 (MQ=255)
                                                                                           tttCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTcgcgcg                                                            >  2:261711/1‑150 (MQ=255)
                                                                                              cGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGtt                                                         >  2:55071/1‑150 (MQ=255)
                                                                                              cGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGtt                                                         >  2:2479650/1‑150 (MQ=255)
                                                                                               gTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTATCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTtc                                                        >  2:870757/1‑150 (MQ=255)
                                                                                                ttttATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTtca                                                       >  2:2093610/1‑149 (MQ=255)
                                                                                                  ttATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCg                                                     >  2:2978222/1‑150 (MQ=255)
                                                                                                   tATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCgg                                                    >  2:2880435/1‑150 (MQ=255)
                                                                                                     tttGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGaa                                                  <  2:3550076/150‑1 (MQ=255)
                                                                                                        gATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAAcg                                               >  2:139423/1‑150 (MQ=255)
                                                                                                        gATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAAcg                                               >  2:139405/1‑150 (MQ=255)
                                                                                                              tGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGc                                         >  1:2159148/1‑150 (MQ=255)
                                                                                                               ggggACTTGGGAATTAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTAGTTTTATAGGTGAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGcc                                        >  1:3430324/1‑150 (MQ=255)
                                                                                                               ggggAATTGGGAAGTAATACTTGAAGACGAAAGGGCCTAGTGATACGCCTAATTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGcc                                        <  2:4160479/150‑1 (MQ=255)
                                                                                                                   aattgggaattAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGt                                    >  1:4084693/1‑150 (MQ=255)
                                                                                                                    attgggaattAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGtt                                   >  1:825940/1‑150 (MQ=255)
                                                                                                                     ttgggaattAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGttt                                  <  2:3444178/150‑1 (MQ=255)
                                                                                                                      tgggaattAATTCGTGACGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTc                                 <  2:3763978/150‑1 (MQ=255)
                                                                                                                       gggaattAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCg                                >  2:439644/1‑150 (MQ=255)
                                                                                                                       gggaattAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCg                                <  2:3185941/150‑1 (MQ=255)
                                                                                                                       gggaattAATTCTTGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCAGGGGAAGATCCAGACGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCg                                >  2:3790548/1‑150 (MQ=255)
                                                                                                                             taattCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTg                          >  1:3125133/1‑150 (MQ=255)
                                                                                                                                ttCTAGAAGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCa                       <  1:495098/150‑1 (MQ=255)
                                                                                                                                 tCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCAc                      >  1:1041529/1‑150 (MQ=255)
                                                                                                                                   ttGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGc                    >  2:1052820/1‑150 (MQ=255)
                                                                                                                                      aaGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCa                 >  2:3918955/1‑150 (MQ=255)
                                                                                                                                      aaGACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCa                 >  1:879956/1‑150 (MQ=255)
                                                                                                                                        gACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAg               >  1:327160/1‑150 (MQ=255)
                                                                                                                                        gACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAg               <  2:1372232/150‑1 (MQ=255)
                                                                                                                                        gACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAg               >  1:326947/1‑150 (MQ=255)
                                                                                                                                        gACGAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAg               <  1:253728/150‑1 (MQ=255)
                                                                                                                                           gAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAGccc            >  1:2196048/1‑150 (MQ=255)
                                                                                                                                           gAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAGccc            <  1:3302752/150‑1 (MQ=255)
                                                                                                                                           gAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAGccc            >  2:1500669/1‑150 (MQ=255)
                                                                                                                                           gAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAGccc            >  2:1755648/1‑150 (MQ=255)
                                                                                                                                           gAAAGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAGccc            >  2:1755865/1‑150 (MQ=255)
                                                                                                                                            aaaGGGCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAGCCCg           >  1:1764309/1‑150 (MQ=255)
                                                                                                                                             aaGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAGCCCGt          >  1:2816205/1‑150 (MQ=255)
                                                                                                                                               gggCCTAGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAGCCCGTAg        >  2:565625/1‑150 (MQ=255)
                                                                                                                                                     cGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAGCCCGTAGTCCCGt  >  2:2897512/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGAAGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTGATTGTCACGCTCAAGCCCGTAGTCCCGT  >  CP009273/360168‑360466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A