Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 362,159 G→T 94.0% I165I (ATC→ATA)  mhpR ← mhp operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273362,1590GT94.0% 708.1 / 32.3 236I165I (ATC→ATA) mhpRmhp operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (8/7);  new base T (99/122);  total (107/129)
Fisher's exact test for biased strand distribution p-value = 5.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

GGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATT  >  CP009273/362012‑362303
                                                                                                                                                   |                                                                                                                                                
ggCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTAt                                                                                                                                                <  2:4636539/150‑1 (MQ=255)
ggCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGAt                                                                                                                                                <  1:2471360/150‑1 (MQ=255)
ggCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGAt                                                                                                                                                >  1:4198362/1‑150 (MQ=255)
   gATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTaa                                                                                                                                             <  1:1977269/150‑1 (MQ=255)
     tAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAAtt                                                                                                                                           <  1:1164250/150‑1 (MQ=255)
     tAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAAtt                                                                                                                                           >  1:273683/1‑150 (MQ=255)
        agGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCt                                                                                                                                        >  1:1462892/1‑150 (MQ=255)
        agGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCt                                                                                                                                        <  2:4486806/150‑1 (MQ=255)
        agGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCt                                                                                                                                        <  1:4571018/150‑1 (MQ=255)
         ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCtt                                                                                                                                       >  2:1052950/1‑150 (MQ=255)
          gCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTg                                                                                                                                      <  2:310753/150‑1 (MQ=255)
            gATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCg                                                                                                                                    <  1:785461/150‑1 (MQ=255)
            gATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCGTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTACTTCCTTGCg                                                                                                                                    <  2:2846335/150‑1 (MQ=255)
                 tctcCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCtt                                                                                                                               <  2:445248/150‑1 (MQ=255)
                    cctcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAAGTCCTTGCGGTCTTGtt                                                                                                                            <  2:4613313/150‑1 (MQ=255)
                     ctcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTc                                                                                                                           <  1:3124617/150‑1 (MQ=255)
                       cctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCg                                                                                                                         >  2:23550/1‑150 (MQ=255)
                         tGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCggg                                                                                                                       >  2:1777973/1‑150 (MQ=255)
                         tGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCggg                                                                                                                       <  2:3520443/150‑1 (MQ=255)
                          gATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGc                                                                                                                      >  1:1800488/1‑150 (MQ=255)
                          gATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGc                                                                                                                      >  1:455668/1‑150 (MQ=255)
                           aTCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCa                                                                                                                     >  2:4263592/1‑150 (MQ=255)
                            tCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCaa                                                                                                                    <  2:1456369/150‑1 (MQ=255)
                             cccAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCaaa                                                                                                                   >  2:1302229/1‑150 (MQ=255)
                             cccAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCaaa                                                                                                                   >  1:2665177/1‑150 (MQ=255)
                             cccAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCaaa                                                                                                                   <  1:1284684/150‑1 (MQ=255)
                                   cgcgGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgcca                                                                                                             <  1:2239821/150‑1 (MQ=255)
                                      ggTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcc                                                                                                          >  2:1820449/1‑150 (MQ=255)
                                      ggTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcc                                                                                                          <  1:4149535/150‑1 (MQ=255)
                                      ggTAGTACTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTAAAAGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATATCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagca                                                                                                          >  1:2360546/1‑149 (MQ=255)
                                       gTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcca                                                                                                         >  1:2857233/1‑150 (MQ=255)
                                       gTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcca                                                                                                         <  1:416445/150‑1 (MQ=255)
                                        tAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGccagccag                                                                                                        >  1:1447974/1‑150 (MQ=255)
                                        tAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGccagccag                                                                                                        >  1:2354951/1‑150 (MQ=255)
                                          gTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGt                                                                                                      >  2:3071581/1‑150 (MQ=255)
                                                tCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCGTCCAGCTTTAACGGTTCCCGTGCCAGTTGAGAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGcc                                                                                                <  2:37903/150‑1 (MQ=255)
                                                 ccGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGGTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGccc                                                                                               <  2:4406074/150‑1 (MQ=255)
                                                   gTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGa                                                                                             >  1:3785139/1‑150 (MQ=255)
                                                             ttGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTc                                                                                   >  2:4351976/1‑150 (MQ=255)
                                                             ttGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTc                                                                                   <  1:2139222/150‑1 (MQ=255)
                                                                cgcgcgcgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGa                                                                                >  1:4245412/1‑150 (MQ=255)
                                                                    cgcgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCg                                                                            >  1:4466244/1‑150 (MQ=255)
                                                                    cgcgcgcCAGAATGGCTCCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCg                                                                            <  1:4262450/150‑1 (MQ=255)
                                                                      cgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGc                                                                          >  2:3881989/1‑150 (MQ=255)
                                                                         gcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCaaa                                                                       >  1:1095806/1‑150 (MQ=255)
                                                                          ccAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAAGTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAAc                                                                      <  1:2320701/150‑1 (MQ=255)
                                                                          ccAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAAc                                                                      <  1:1552973/150‑1 (MQ=255)
                                                                              aaTGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCg                                                                  <  2:2705017/150‑1 (MQ=255)
                                                                                tGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGcc                                                                <  2:4130989/150‑1 (MQ=255)
                                                                                   cTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGa                                                             <  1:870414/150‑1 (MQ=255)
                                                                                    ttCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGAc                                                            >  2:3934406/1‑150 (MQ=255)
                                                                                       cAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCAt                                                         <  2:2756043/150‑1 (MQ=255)
                                                                                         gCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTg                                                       >  1:1971557/1‑150 (MQ=255)
                                                                                          cTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGc                                                      >  1:2459908/1‑150 (MQ=255)
                                                                                               aCGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGt                                                 <  2:1230494/150‑1 (MQ=255)
                                                                                                 ggTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGa                                               <  2:1154776/150‑1 (MQ=255)
                                                                                                 ggTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGa                                               <  2:3557893/150‑1 (MQ=255)
                                                                                                 ggTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGa                                               >  2:2469450/1‑150 (MQ=255)
                                                                                                   ttCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGaaa                                             >  2:2589171/1‑150 (MQ=255)
                                                                                                       cGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGAt                                         <  1:2062937/150‑1 (MQ=255)
                                                                                                        gTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCCAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGACGCGGCAAACGTCGCCCGACCATTGCCCGGTGACAGGATa                                        >  2:3659115/1‑150 (MQ=255)
                                                                                                        gTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATa                                        >  2:3248469/1‑150 (MQ=255)
                                                                                                        gTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATa                                        >  2:4200817/1‑150 (MQ=255)
                                                                                                        gTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAAAATTTCGATTAATTCCTTGCGGTCATGTTCCGGGCAAAAGGCCAGCCATGTCAGGCCCTAGGCGGTTTTCAGAAGAGGCAAACGTCGCCCGAACAATGCCCGGTGAAAGGATa                                        >  2:1872306/1‑150 (MQ=255)
                                                                                                         tGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATaa                                       <  2:3669578/150‑1 (MQ=255)
                                                                                                          gCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAg                                      <  2:564812/150‑1 (MQ=255)
                                                                                                          gCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAg                                      <  2:2138640/150‑1 (MQ=255)
                                                                                                           ccAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGc                                     >  1:699226/1‑150 (MQ=255)
                                                                                                               ttGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCt                                 <  1:1257567/150‑1 (MQ=255)
                                                                                                               ttGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCt                                 <  2:1940716/150‑1 (MQ=255)
                                                                                                                tGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTg                                <  1:1666832/150‑1 (MQ=255)
                                                                                                                 gATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGa                               >  2:2473059/1‑150 (MQ=255)
                                                                                                                   tAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGaaa                             <  2:347953/150‑1 (MQ=255)
                                                                                                                    aGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGaaat                            <  1:3446944/150‑2 (MQ=255)
                                                                                                                        atcaCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTg                        <  2:1668576/150‑1 (MQ=255)
                                                                                                                        atcaCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTg                        <  2:2439704/150‑1 (MQ=255)
                                                                                                                         tcaCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGa                       <  1:1315744/150‑1 (MQ=255)
                                                                                                                           tccGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGGTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGt                     <  1:2954518/149‑1 (MQ=255)
                                                                                                                           aCCGGGGCGGGAGTCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGt                     <  2:2659567/150‑1 (MQ=255)
                                                                                                                           aCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGt                     >  2:2004589/1‑150 (MQ=255)
                                                                                                                           aCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGt                     >  2:2288332/1‑150 (MQ=255)
                                                                                                                           aCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGt                     >  2:2398172/1‑150 (MQ=255)
                                                                                                                           aCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGt                     >  1:3005631/1‑150 (MQ=255)
                                                                                                                            ccGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTg                    <  2:4546389/150‑1 (MQ=255)
                                                                                                                            ccGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTg                    <  1:1842825/150‑1 (MQ=255)
                                                                                                                             cGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTgg                   <  1:2994064/150‑1 (MQ=255)
                                                                                                                             cGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTgg                   <  2:4038099/150‑1 (MQ=255)
                                                                                                                              ggggCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGt                  >  1:3098199/1‑150 (MQ=255)
                                                                                                                              ggggCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGt                  >  1:1121993/1‑150 (MQ=255)
                                                                                                                                 gCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTc               <  2:1476857/150‑1 (MQ=255)
                                                                                                                                 gCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTc               <  2:187017/150‑1 (MQ=255)
                                                                                                                                  cGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAATCGGCTGAAACGGTGAGTGGTTTcg              >  1:3472896/1‑150 (MQ=255)
                                                                                                                                  cGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTcg              <  2:3144354/150‑1 (MQ=255)
                                                                                                                                   gggAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTcgc             >  1:391728/1‑150 (MQ=255)
                                                                                                                                   gggAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTcgc             <  1:949030/150‑1 (MQ=255)
                                                                                                                                   gggAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTcgc             >  2:2505889/1‑150 (MQ=255)
                                                                                                                                     gAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGt           >  2:374521/1‑150 (MQ=255)
                                                                                                                                      aGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTa          >  1:616798/1‑150 (MQ=255)
                                                                                                                                         cTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTacca       >  1:669737/1‑150 (MQ=255)
                                                                                                                                              aTTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCAtt  <  2:531360/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATT  >  CP009273/362012‑362303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTGCATC  >  CP009273/362011‑362308
                                                                                                                                                    |                                                                                                                                                     
CGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTA                                                                                                                                                      >  SRR19973073.1870794/1‑150 (MQ=60)
CGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTA                                                                                                                                                      >  SRR19973073.1870801/1‑150 (MQ=60)
CGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTA                                                                                                                                                      <  SRR19973073.2607898/150‑1 (MQ=60)
CGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTA                                                                                                                                                      <  SRR19973073.4334598/150‑1 (MQ=60)
 GGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGAT                                                                                                                                                     <  SRR19973073.2476467/150‑1 (MQ=60)
 GGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGAT                                                                                                                                                     >  SRR19973073.4206698/1‑150 (MQ=60)
 GGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGAT                                                                                                                                                     >  SRR19973073.4206733/1‑150 (MQ=60)
 GGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGAT                                                                                                                                                     <  SRR19973073.2476475/150‑1 (MQ=60)
   CGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTA                                                                                                                                                   <  SRR19973073.1850557/150‑1 (MQ=60)
    GATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAA                                                                                                                                                  <  SRR19973073.1981361/150‑1 (MQ=60)
    GATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAA                                                                                                                                                  <  SRR19973073.1981365/150‑1 (MQ=60)
      TAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATT                                                                                                                                                >  SRR19973073.274289/1‑150 (MQ=60)
      TAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATT                                                                                                                                                >  SRR19973073.274278/1‑150 (MQ=60)
      TAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATT                                                                                                                                                <  SRR19973073.1166642/150‑1 (MQ=60)
      TAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATT                                                                                                                                                <  SRR19973073.1166625/150‑1 (MQ=60)
      TAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATT                                                                                                                                                <  SRR19973073.1166607/150‑1 (MQ=60)
         AGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCT                                                                                                                                             >  SRR19973073.1465958/1‑150 (MQ=60)
         AGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCT                                                                                                                                             <  SRR19973073.4580159/150‑1 (MQ=60)
             GATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCG                                                                                                                                         <  SRR19973073.2727905/150‑1 (MQ=60)
             GATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCG                                                                                                                                         <  SRR19973073.787048/150‑1 (MQ=60)
             GATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCG                                                                                                                                         <  SRR19973073.3108712/150‑1 (MQ=60)
              ATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGG                                                                                                                                        <  SRR19973073.770305/150‑1 (MQ=60)
                 TTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCT                                                                                                                                     >  SRR19973073.2935219/1‑150 (MQ=60)
                      CTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTC                                                                                                                                <  SRR19973073.808248/150‑1 (MQ=60)
                      CTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTC                                                                                                                                <  SRR19973073.1946313/150‑1 (MQ=60)
                      CTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTC                                                                                                                                <  SRR19973073.3130903/150‑1 (MQ=60)
                        CCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCG                                                                                                                              <  SRR19973073.4209534/150‑1 (MQ=60)
                           GATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGC                                                                                                                           >  SRR19973073.1804204/1‑150 (MQ=60)
                           GATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGC                                                                                                                           >  SRR19973073.1804615/1‑150 (MQ=60)
                           GATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGC                                                                                                                           >  SRR19973073.2533325/1‑150 (MQ=60)
                           GATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGC                                                                                                                           >  SRR19973073.456609/1‑150 (MQ=60)
                           GATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTATAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGATCCGGGC                                                                                                                           >  SRR19973073.456964/1‑150 (MQ=60)
                              CCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAA                                                                                                                        <  SRR19973073.1287386/150‑1 (MQ=60)
                              CCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAA                                                                                                                        >  SRR19973073.2670629/1‑150 (MQ=60)
                              CCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAA                                                                                                                        <  SRR19973073.1287311/150‑1 (MQ=60)
                              CCCAGCCGCGGGAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAA                                                                                                                        <  SRR19973073.1287486/150‑1 (MQ=60)
                                CAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAG                                                                                                                      >  SRR19973073.4591159/1‑150 (MQ=60)
                                    CGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCA                                                                                                                  <  SRR19973073.2244417/150‑1 (MQ=60)
                                    CGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCA                                                                                                                  <  SRR19973073.2244439/150‑1 (MQ=60)
                                    CGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCA                                                                                                                  <  SRR19973073.2244441/150‑1 (MQ=60)
                                       GGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCC                                                                                                               <  SRR19973073.4157829/150‑1 (MQ=60)
                                       GGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCC                                                                                                               <  SRR19973073.4157827/150‑1 (MQ=60)
                                       GGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCC                                                                                                               <  SRR19973073.3152779/150‑1 (MQ=60)
                                       GGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCC                                                                                                               >  SRR19973073.3876457/1‑150 (MQ=60)
                                       GGTAGTACTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTAAAAGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATATCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCA                                                                                                               >  SRR19973073.2365373/1‑150 (MQ=60)
                                        GTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCA                                                                                                              >  SRR19973073.2863086/1‑150 (MQ=60)
                                        GTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCA                                                                                                              <  SRR19973073.417276/150‑1 (MQ=60)
                                        GTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCA                                                                                                              <  SRR19973073.1105298/150‑1 (MQ=60)
                                        GTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCA                                                                                                              <  SRR19973073.2260715/150‑1 (MQ=60)
                                         TAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAG                                                                                                             >  SRR19973073.2359763/1‑150 (MQ=60)
                                         TAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAG                                                                                                             >  SRR19973073.2359985/1‑150 (MQ=60)
                                         TAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAG                                                                                                             >  SRR19973073.1451044/1‑150 (MQ=60)
                                         TAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAG                                                                                                             >  SRR19973073.1451018/1‑150 (MQ=60)
                                                   CGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCG                                                                                                   >  SRR19973073.3454784/1‑150 (MQ=60)
                                                   CGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCG                                                                                                   <  SRR19973073.4131807/150‑1 (MQ=60)
                                                    GTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGA                                                                                                  >  SRR19973073.3792714/1‑150 (MQ=60)
                                                         CCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGG                                                                                             >  SRR19973073.3072739/1‑150 (MQ=60)
                                                         CCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGG                                                                                             >  SRR19973073.3072644/1‑150 (MQ=60)
                                                             TTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTT                                                                                         <  SRR19973073.3559795/150‑1 (MQ=60)
                                                              TTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTC                                                                                        <  SRR19973073.2143700/150‑1 (MQ=60)
                                                                 CGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGA                                                                                     >  SRR19973073.4253813/1‑150 (MQ=60)
                                                                     CGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCG                                                                                 >  SRR19973073.4475225/1‑150 (MQ=60)
                                                                     CGCGCGCCAGAATGGCTCCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCG                                                                                 <  SRR19973073.4270894/150‑1 (MQ=60)
                                                                          GCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAA                                                                            >  SRR19973073.1098056/1‑150 (MQ=60)
                                                                           CCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAAGTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAAC                                                                           <  SRR19973073.2325424/150‑1 (MQ=60)
                                                                           CCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAAC                                                                           <  SRR19973073.1556153/150‑1 (MQ=60)
                                                                                  GGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCC                                                                    >  SRR19973073.47828/1‑150 (MQ=60)
                                                                                    CTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGA                                                                  <  SRR19973073.872217/150‑1 (MQ=60)
                                                                                        CAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCAT                                                              <  SRR19973073.4180031/150‑1 (MQ=60)
                                                                                          GCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG                                                            >  SRR19973073.1975641/1‑150 (MQ=60)
                                                                                           CTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGC                                                           >  SRR19973073.2465005/1‑150 (MQ=60)
                                                                                           CTTAAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGC                                                           >  SRR19973073.2464956/1‑150 (MQ=60)
                                                                                                      CCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGG                                                <  SRR19973073.1535048/150‑1 (MQ=60)
                                                                                                        CGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGAT                                              <  SRR19973073.2067233/150‑1 (MQ=60)
                                                                                                        CGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGAT                                              <  SRR19973073.426016/150‑1 (MQ=60)
                                                                                                            CCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGC                                          >  SRR19973073.700679/1‑150 (MQ=60)
                                                                                                            CCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGC                                          >  SRR19973073.700667/1‑150 (MQ=60)
                                                                                                            CCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGC                                          >  SRR19973073.700684/1‑150 (MQ=60)
                                                                                                              AGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGG                                        <  SRR19973073.2931825/150‑1 (MQ=60)
                                                                                                                TTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCT                                      <  SRR19973073.1260199/150‑1 (MQ=60)
                                                                                                                 TGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTG                                     >  SRR19973073.994882/1‑150 (MQ=60)
                                                                                                                 TGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTG                                     <  SRR19973073.1670295/150‑1 (MQ=60)
                                                                                                                     AGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAAT                                 <  SRR19973073.3453814/150‑1 (MQ=60)
                                                                                                                     AGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAAC                                 <  SRR19973073.3453822/150‑1 (MQ=60)
                                                                                                                        CATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGT                              >  SRR19973073.4306104/1‑150 (MQ=60)
                                                                                                                         ATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTG                             >  SRR19973073.2093066/1‑150 (MQ=60)
                                                                                                                          TCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGA                            <  SRR19973073.1318530/150‑1 (MQ=60)
                                                                                                                          TCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGA                            <  SRR19973073.1318533/150‑1 (MQ=60)
                                                                                                                            TCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGGTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGT                          <  SRR19973073.2960540/150‑1 (MQ=60)
                                                                                                                            ACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGT                          >  SRR19973073.3011730/1‑150 (MQ=60)
                                                                                                                            ACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGT                          >  SRR19973073.3011653/1‑150 (MQ=60)
                                                                                                                             CCGGGGCGGGAGGCTATCATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTG                         <  SRR19973073.2453869/150‑1 (MQ=60)
                                                                                                                             CCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTG                         <  SRR19973073.1846621/150‑1 (MQ=60)
                                                                                                                             CCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTG                         <  SRR19973073.137235/150‑1 (MQ=60)
                                                                                                                             CCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTG                         <  SRR19973073.1991463/150‑1 (MQ=60)
                                                                                                                              CGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGG                        <  SRR19973073.3000147/150‑1 (MQ=60)
                                                                                                                              CGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGG                        <  SRR19973073.2999963/150‑1 (MQ=60)
                                                                                                                               GGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGT                       >  SRR19973073.3104426/1‑150 (MQ=60)
                                                                                                                               GGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGT                       >  SRR19973073.1124282/1‑150 (MQ=60)
                                                                                                                                   CGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAATCGGCTGAAACGGTGAGTGGTTTCG                   >  SRR19973073.3479796/1‑150 (MQ=60)
                                                                                                                                   CGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCG                   >  SRR19973073.4334228/1‑150 (MQ=60)
                                                                                                                                    GGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGC                  >  SRR19973073.392509/1‑150 (MQ=60)
                                                                                                                                    GGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGC                  <  SRR19973073.951004/150‑1 (MQ=60)
                                                                                                                                    GGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGC                  <  SRR19973073.951056/150‑1 (MQ=60)
                                                                                                                                    GGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGC                  <  SRR19973073.951009/150‑1 (MQ=60)
                                                                                                                                      GAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGT                >  SRR19973073.3941320/1‑150 (MQ=60)
                                                                                                                                       AGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTA               >  SRR19973073.233900/1‑150 (MQ=60)
                                                                                                                                       AGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTA               >  SRR19973073.618117/1‑150 (MQ=60)
                                                                                                                                       AGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTA               >  SRR19973073.1997449/1‑150 (MQ=60)
                                                                                                                                         GCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACC             >  SRR19973073.4546566/1‑150 (MQ=60)
                                                                                                                                          CTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCA            >  SRR19973073.671096/1‑150 (MQ=60)
                                                                                                                                          CTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCA            >  SRR19973073.671132/1‑150 (MQ=60)
                                                                                                                                          CTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCA            <  SRR19973073.2742139/150‑1 (MQ=60)
                                                                                                                                           TAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCAC           >  SRR19973073.1198073/1‑150 (MQ=60)
                                                                                                                                            AACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACC          <  SRR19973073.1649105/150‑1 (MQ=60)
                                                                                                                                            AACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACC          <  SRR19973073.1649100/150‑1 (MQ=60)
                                                                                                                                               ATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATT       <  SRR19973073.1789588/150‑1 (MQ=60)
                                                                                                                                                    TATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTGCATC  <  SRR19973073.3698538/150‑1 (MQ=60)
                                                                                                                                                    TATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTGCATC  >  SRR19973073.1178946/1‑150 (MQ=60)
                                                                                                                                                    TATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTGCATC  >  SRR19973073.1178969/1‑150 (MQ=60)
                                                                                                                                                    TATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTGCATC  >  SRR19973073.4523541/1‑150 (MQ=60)
                                                                                                                                                    TATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGACAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTGCATC  >  SRR19973073.1178945/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTGCATC  >  CP009273/362011‑362308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑