Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 815,168 C→A 15.1% R140R (CGG→AGG)  moaE → molybdopterin synthase, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273815,1680CA15.1% 944.4 / 94.1 286R140R (CGG→AGG) moaEmolybdopterin synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base C (119/123);  new base A (14/30);  total (133/153)
Fisher's exact test for biased strand distribution p-value = 4.78e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 294 total aligned reads displayed.

CGGGCGATGAAATCGTTTTTGTCGGTGTCACCAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTC  >  CP009273/815019‑815317
                                                                                                                                                     |                                                                                                                                                     
    cGATGAAATCGTTTTTGTCGGTGTCACCAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGga                                                                                                                                                   >  1:3640090/1‑150 (MQ=255)
    cGATGAAATCGTTTTTGTCGGTGTCACCAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGga                                                                                                                                                   >  1:1393908/1‑150 (MQ=255)
      aTGAAATCGTTTTTGTCGGTGTCACCAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGgaga                                                                                                                                                 <  2:1057815/150‑1 (MQ=255)
       tGAAATCGTTTTTGTCGGTGTCACCAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGagag                                                                                                                                                <  1:618544/150‑1 (MQ=255)
       tGAAATCGTTTTTGTCGGTGTCACCAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGagag                                                                                                                                                <  1:2277111/150‑1 (MQ=255)
       tGAAATCGTTTTTGTCGGTGTCACCAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGagag                                                                                                                                                >  1:2963852/1‑150 (MQ=255)
        gAAATCGTTTTTGTCGGTGTCACCAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGc                                                                                                                                               >  1:4193748/1‑150 (MQ=255)
         aaaTCGTTTTTGTCGGTGTCACCAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCACAAAACCCGCGCACCGTTCTGGAAGCGCGACGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCg                                                                                                                                              <  1:3935389/150‑1 (MQ=255)
          aaTCGTTTTTGTCGGTGTCACCCGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGa                                                                                                                                             <  1:4460420/150‑1 (MQ=255)
           aTCGTTTTTGTCGGTGTCACCAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGCGAGCGAt                                                                                                                                            >  1:4992/1‑150 (MQ=255)
            tCGTTTTTGTCGGTGTCACCAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATc                                                                                                                                           <  1:1815914/150‑1 (MQ=255)
              gTTTTTGTCGGTGTCACCAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATcag                                                                                                                                         <  2:3521308/150‑1 (MQ=255)
                ttttGTCGGTGTCACCAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATcagca                                                                                                                                       >  2:844272/1‑150 (MQ=255)
                   tGTCGGTGTCACCAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAggc                                                                                                                                    >  2:1202558/1‑150 (MQ=255)
                   tGTCGGTGTCACCAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAggc                                                                                                                                    >  2:60548/1‑150 (MQ=255)
                         tgtCACCAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTAGGGAGAGCGATCAGCAGGCGGCaaa                                                                                                                              <  2:4244808/150‑1 (MQ=255)
                             aCCAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTAGGGAGAGCGATCAGCAGGCGGCAAAACGc                                                                                                                          >  2:2196529/1‑150 (MQ=255)
                              ccAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTAGGGAGAGCGATCAGCAGGCGGCAAAACGCt                                                                                                                         >  1:1034397/1‑150 (MQ=255)
                               cAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTg                                                                                                                        >  2:3845881/1‑150 (MQ=255)
                               cAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTg                                                                                                                        >  1:1153467/1‑150 (MQ=255)
                                aGTGCGCATCGCAGAAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTAGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg                                                                                                                       <  1:1973986/150‑1 (MQ=255)
                                     gcATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGtt                                                                                                                  <  1:2457098/150‑1 (MQ=255)
                                     gcATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGtt                                                                                                                  >  1:2844430/1‑150 (MQ=255)
                                     gcATCGCAACAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGtt                                                                                                                  <  1:4593428/150‑1 (MQ=255)
                                         cGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGtttttt                                                                                                              >  2:490018/1‑150 (MQ=255)
                                         cGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGtttttt                                                                                                              >  2:3369101/1‑150 (MQ=255)
                                          gcagcaGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTg                                                                                                             >  2:4329561/1‑150 (MQ=255)
                                            agcagTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCACAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTAGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGtt                                                                                                           <  2:2724143/150‑1 (MQ=255)
                                            agcagTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTAGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGtt                                                                                                           <  2:3471997/150‑1 (MQ=255)
                                            agcagTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTAGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGtt                                                                                                           <  2:1154176/150‑1 (MQ=255)
                                             gcagTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTa                                                                                                          <  1:623637/150‑1 (MQ=255)
                                                  gCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCgg                                                                                                     >  1:4410431/1‑150 (MQ=255)
                                                    gTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGAt                                                                                                   <  2:1724290/150‑1 (MQ=255)
                                                     tttGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATa                                                                                                  >  2:1040101/1‑150 (MQ=255)
                                                        gAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAgg                                                                                               <  1:3292175/150‑1 (MQ=255)
                                                         aaGCCGGGCAGTTTATTATGGATTATCTCANAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGc                                                                                              >  1:4276470/1‑150 (MQ=255)
                                                         aaGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGc                                                                                              >  2:4169257/1‑150 (MQ=255)
                                                         aaGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGc                                                                                              >  2:4022211/1‑150 (MQ=255)
                                                         aaGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTAGGGAGAGCGATCAGCAGGCGGCAAAACGCGGGTAGTTATTTGTTAGCCGGATAAgcc                                                                                              >  1:399448/1‑148 (MQ=255)
                                                           gCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCAc                                                                                            >  1:4526398/1‑150 (MQ=255)
                                                            ccGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCAcc                                                                                           <  1:1019581/150‑1 (MQ=255)
                                                            ccGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCAcc                                                                                           <  2:24381/150‑1 (MQ=255)
                                                                 cAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTAGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGc                                                                                      <  1:2990798/150‑1 (MQ=255)
                                                                   gTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGt                                                                                    >  1:2370287/1‑150 (MQ=255)
                                                                   gTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGt                                                                                    >  1:21363/1‑150 (MQ=255)
                                                                     ttattaTGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTAGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTcc                                                                                  <  1:4126786/150‑1 (MQ=255)
                                                                       attatGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCgg                                                                                <  2:540654/150‑1 (MQ=255)
                                                                       attatGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCgg                                                                                >  2:315440/1‑150 (MQ=255)
                                                                       attatGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCgg                                                                                <  2:198986/150‑1 (MQ=255)
                                                                        ttatGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGc                                                                               >  2:4282013/1‑150 (MQ=255)
                                                                         tatGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCa                                                                              >  1:214769/1‑150 (MQ=255)
                                                                           tGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAAt                                                                            >  1:2064476/1‑150 (MQ=255)
                                                                            ggATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAAtt                                                                           <  2:1154719/150‑1 (MQ=255)
                                                                             gATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTAGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAAttt                                                                          <  1:2901490/150‑1 (MQ=255)
                                                                               ttATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAATGCACCGCTGCGTCCGGCAATTTCa                                                                        >  2:596385/1‑150 (MQ=255)
                                                                                 aTCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCAcc                                                                      <  2:4214091/150‑1 (MQ=255)
                                                                                 aTCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCAcc                                                                      >  1:921310/1‑150 (MQ=255)
                                                                                 aTCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTAGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCAcc                                                                      <  2:3554932/150‑1 (MQ=255)
                                                                                  tctcAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCg                                                                     >  2:3274027/1‑150 (MQ=255)
                                                                                    tcAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCt                                                                   <  1:705630/150‑1 (MQ=255)
                                                                                      aaaaCCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCa                                                                 <  2:15742/150‑1 (MQ=255)
                                                                                       aaaCCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAg                                                                <  1:3846486/150‑1 (MQ=255)
                                                                                          cccGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTAc                                                             >  1:3816962/1‑150 (MQ=255)
                                                                                           ccGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACa                                                            <  2:1797746/150‑1 (MQ=255)
                                                                                                 aCCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGtt                                                      <  1:3118778/150‑1 (MQ=255)
                                                                                                 aCCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGtt                                                      <  1:1365975/150‑1 (MQ=255)
                                                                                                   cGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTa                                                    <  2:2940256/150‑1 (MQ=255)
                                                                                                    gTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTAGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTAc                                                   <  1:3501360/150‑1 (MQ=255)
                                                                                                     ttCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGATGGTAGTTTTTTGTTAGCCGGATAAGGAACCGCTGCGTCCGGCAATTTCACCGCTCAGTAAATACGTTTACg                                                  >  2:2526188/1‑150 (MQ=255)
                                                                                                       cTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCt                                                <  1:1667581/150‑1 (MQ=255)
                                                                                                            aGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTc                                           <  2:24964/150‑1 (MQ=255)
                                                                                                                cgAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATg                                       <  2:3699979/150‑1 (MQ=255)
                                                                                                                cgAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATg                                       <  1:1498439/150‑1 (MQ=255)
                                                                                                                 gAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGc                                      >  2:4305262/1‑150 (MQ=255)
                                                                                                                 gAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTAGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGc                                      >  2:2359195/1‑150 (MQ=255)
                                                                                                                  aaGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGca                                     <  1:2017497/150‑1 (MQ=255)
                                                                                                                   aGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGcac                                    >  2:4378458/1‑150 (MQ=255)
                                                                                                                    gCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGcaca                                   <  2:2849132/150‑1 (MQ=255)
                                                                                                                    gCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGcaca                                   >  2:1301629/1‑150 (MQ=255)
                                                                                                                     ccACGCCGGAAGGCGACCGCTGGGTTGAAGCTAGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAg                                  <  1:1614326/150‑1 (MQ=255)
                                                                                                                      cACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGc                                 <  1:3213046/150‑1 (MQ=255)
                                                                                                                       aCGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGcc                                >  1:1105391/1‑150 (MQ=255)
                                                                                                                       aCGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGcc                                >  1:3423656/1‑150 (MQ=255)
                                                                                                                         gCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCtc                              <  2:2628216/150‑1 (MQ=255)
                                                                                                                          ccGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCtca                             <  1:277095/150‑1 (MQ=255)
                                                                                                                            ggAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCtcatc                           >  1:1270469/1‑150 (MQ=255)
                                                                                                                             gAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCtcatca                          >  2:746336/1‑150 (MQ=255)
                                                                                                                               aGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCAtt                        >  2:3448314/1‑150 (MQ=255)
                                                                                                                                 gCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCAtttt                      <  1:3529859/150‑1 (MQ=255)
                                                                                                                                  cGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTg                     >  2:307350/1‑150 (MQ=255)
                                                                                                                                      cGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGt                 <  2:211462/150‑1 (MQ=255)
                                                                                                                                          gggTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATcc             <  1:1469041/150‑1 (MQ=255)
                                                                                                                                              tGAAGCTAGGGAGAGCGAACAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTaa         <  2:4568443/150‑1 (MQ=255)
                                                                                                                                               gAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAg        >  1:2919321/1‑150 (MQ=255)
                                                                                                                                               gAAGCTAGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAg        <  1:1712396/150‑1 (MQ=255)
                                                                                                                                                  gCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGata     <  2:1651511/150‑1 (MQ=255)
                                                                                                                                                     cGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTc  <  1:3556105/150‑1 (MQ=255)
                                                                                                                                                     cGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTc  <  1:804760/150‑1 (MQ=255)
                                                                                                                                                     cGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTc  <  1:2323897/150‑1 (MQ=255)
                                                                                                                                                     cGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGACGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTc  >  2:3011790/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGGCGATGAAATCGTTTTTGTCGGTGTCACCAGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTC  >  CP009273/815019‑815317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A