Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 837,716 G→T 5.8% intergenic (‑204/‑72) mcbA ← / → rlmF colanic acid mucoidy stimulation protein/23S rRNA m(6)A1618 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273837,7160GT 5.8% 1065.8 / 25.7 276intergenic (‑204/‑72)mcbA/rlmFcolanic acid mucoidy stimulation protein/23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (137/123);  new base T (9/7);  total (146/130)
Fisher's exact test for biased strand distribution p-value = 8.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 276 total aligned reads displayed.

CGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTGTCAG  >  CP009273/837568‑837862
                                                                                                                                                    |                                                                                                                                                  
 gATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGAt                                                                                                                                                  <  1:2627357/150‑1 (MQ=255)
  aTGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATa                                                                                                                                                 >  1:4324215/1‑150 (MQ=255)
       aaTGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGc                                                                                                                                            >  1:67973/1‑150 (MQ=255)
         tGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAg                                                                                                                                          >  1:833807/1‑150 (MQ=255)
          gACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGc                                                                                                                                         <  2:1537625/150‑1 (MQ=255)
          gACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGc                                                                                                                                         <  1:1590352/150‑1 (MQ=255)
           aCGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGcc                                                                                                                                        <  1:3418380/150‑1 (MQ=255)
             gCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAAAAAGACAAATCAATGACATGCATAATGATGATTTTTATGATGAATTGCCATAAACCATATCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCat                                                                                                                                      >  2:1246237/1‑149 (MQ=255)
                aaaaaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGata                                                                                                                                   <  2:3064267/150‑1 (MQ=255)
                aaaaaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGata                                                                                                                                   >  1:1529957/1‑150 (MQ=255)
                  aaaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATAtt                                                                                                                                 >  2:2779312/1‑150 (MQ=255)
                   aaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGTCATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATAttt                                                                                                                                <  1:1805428/150‑1 (MQ=255)
                   aaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATAttt                                                                                                                                >  2:528366/1‑150 (MQ=255)
                   aaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATAttt                                                                                                                                <  1:3445549/150‑1 (MQ=255)
                   aaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATAttt                                                                                                                                >  2:4010462/1‑150 (MQ=255)
                   aaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATGGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATAttt                                                                                                                                <  2:1439951/150‑1 (MQ=255)
                     ttGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTAc                                                                                                                              <  1:2419568/150‑1 (MQ=255)
                                gtgCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGtt                                                                                                                   <  2:514207/150‑1 (MQ=255)
                                gtgCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGtt                                                                                                                   <  2:913246/150‑1 (MQ=255)
                                 tgCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTa                                                                                                                  <  2:1186918/150‑1 (MQ=255)
                                  gCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTAt                                                                                                                 >  1:3521032/1‑150 (MQ=255)
                                  gCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTAt                                                                                                                 <  2:4064141/150‑1 (MQ=255)
                                   cTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATg                                                                                                                >  1:3112249/1‑150 (MQ=255)
                                   cTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATg                                                                                                                <  1:2754401/150‑1 (MQ=255)
                                       ttttttGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCggg                                                                                                            >  1:4271616/1‑150 (MQ=255)
                                        tttttGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAATCCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGt                                                                                                           >  1:1856947/1‑150 (MQ=255)
                                          tttGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTAt                                                                                                         <  2:183491/150‑1 (MQ=255)
                                            tGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCt                                                                                                       <  1:2045749/150‑1 (MQ=255)
                                            tGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCt                                                                                                       >  1:2998169/1‑150 (MQ=255)
                                            tGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCt                                                                                                       <  2:334603/150‑1 (MQ=255)
                                             gAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCtt                                                                                                      >  2:2064572/1‑150 (MQ=255)
                                             gAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACACTGTCGCGTTATGCGGGTATCtt                                                                                                      >  1:3278353/1‑150 (MQ=255)
                                              aaCGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTa                                                                                                     >  1:494701/1‑150 (MQ=255)
                                              aaCGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTa                                                                                                     >  2:1370123/1‑150 (MQ=255)
                                                cGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTAcg                                                                                                   <  2:4234065/150‑1 (MQ=255)
                                                  atatatTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTAcgcc                                                                                                 <  1:4290978/150‑1 (MQ=255)
                                                   tatatTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAAGTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTAcgccg                                                                                                <  2:4358967/150‑1 (MQ=255)
                                                     tatTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCgct                                                                                              >  1:1360171/1‑150 (MQ=255)
                                                      atTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCgctg                                                                                             <  1:1790851/150‑1 (MQ=255)
                                                         tttACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCtt                                                                                          <  1:1645857/150‑1 (MQ=255)
                                                         tttACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCtt                                                                                          <  2:4401661/150‑1 (MQ=255)
                                                           tACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTaa                                                                                        >  1:4111078/1‑150 (MQ=255)
                                                              aaaaTAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAgg                                                                                     >  2:3721685/1‑150 (MQ=255)
                                                              aaaaTAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAgg                                                                                     >  2:4566054/1‑150 (MQ=255)
                                                               taaTAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGga                                                                                    >  2:1628962/2‑150 (MQ=255)
                                                               aaaTAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGga                                                                                    >  1:3955745/1‑150 (MQ=255)
                                                                aaTAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGgag                                                                                   <  2:829765/150‑1 (MQ=255)
                                                                 aTAAGACAAATCAATGACATGCATAATGCCGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGgaga                                                                                  >  1:1964385/1‑150 (MQ=255)
                                                                   aaGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAAt                                                                                <  1:3991131/150‑1 (MQ=255)
                                                                   aaGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAAt                                                                                >  1:3142781/1‑150 (MQ=255)
                                                                    aGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATg                                                                               >  2:4457460/1‑150 (MQ=255)
                                                                     gACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGc                                                                              <  1:461991/150‑1 (MQ=255)
                                                                     gACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGc                                                                              >  1:4451213/1‑150 (MQ=255)
                                                                      aCAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCt                                                                             >  1:2941457/1‑150 (MQ=255)
                                                                       cAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTa                                                                            >  1:3013774/1‑150 (MQ=255)
                                                                       cAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTa                                                                            >  2:420343/1‑150 (MQ=255)
                                                                         aaTCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATg                                                                          >  2:260035/1‑150 (MQ=255)
                                                                          aTCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGt                                                                         >  1:106088/1‑150 (MQ=255)
                                                                            cAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTcc                                                                       >  2:3660185/1‑150 (MQ=255)
                                                                             aaTGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCg                                                                      >  1:3825598/1‑150 (MQ=255)
                                                                               tGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGcc                                                                    <  1:2222506/150‑1 (MQ=255)
                                                                                     gCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGaaa                                                              <  2:479820/150‑1 (MQ=255)
                                                                                          aTGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCggg                                                         >  1:1237942/1‑150 (MQ=255)
                                                                                          aTGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCggg                                                         <  2:2631465/150‑1 (MQ=255)
                                                                                            gCTGATTTTTATGATGAATTGCCATAAACCCTAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGt                                                       <  2:252957/150‑1 (MQ=255)
                                                                                            gCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGt                                                       >  2:3827348/1‑150 (MQ=255)
                                                                                              tGATTTTTATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTg                                                     <  2:1075136/150‑1 (MQ=255)
                                                                                                   tttATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATcc                                                <  2:3943662/150‑1 (MQ=255)
                                                                                                   tttATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATcc                                                <  1:3438682/150‑1 (MQ=255)
                                                                                                   tttATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATcc                                                >  1:22273/1‑150 (MQ=255)
                                                                                                   tttATGATGAATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATcc                                                <  1:3438326/150‑1 (MQ=255)
                                                                                                    ttATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCcg                                               >  2:2106191/1‑150 (MQ=255)
                                                                                                        gatgaATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCa                                           >  2:2462036/1‑150 (MQ=255)
                                                                                                          tgaATTGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAAc                                         >  2:3877765/1‑150 (MQ=255)
                                                                                                            aaTTGCCATAAACAATAGCCATGTTAATGTTTTGGGGAATTATAAACGCAGCCAGATATTTACACTGTCGCTTTATTCGGGTATCTTACGCCGCTGCTTAAAGGATAATGCTATTTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCg                                       >  2:4607294/1‑150 (MQ=255)
                                                                                                              ttGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGtc                                     >  1:1300750/1‑150 (MQ=255)
                                                                                                              ttGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGtc                                     >  1:3547391/1‑150 (MQ=255)
                                                                                                              ttGCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCGGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGACTGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGtc                                     <  1:4430869/150‑1 (MQ=255)
                                                                                                                gCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCCGAAACCGGGGTTGCATCCGCGCAACCGtcat                                   <  2:2262968/150‑1 (MQ=255)
                                                                                                                gCCATAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGtcat                                   <  2:4532543/150‑1 (MQ=255)
                                                                                                                   aTAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATcac                                >  2:4390278/1‑150 (MQ=255)
                                                                                                                   aTAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATcac                                <  1:3695648/150‑1 (MQ=255)
                                                                                                                   aTAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATcac                                <  1:2893736/150‑1 (MQ=255)
                                                                                                                   aTAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATcac                                >  2:2013701/1‑150 (MQ=255)
                                                                                                                   aTAAACCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATcac                                >  2:3495293/1‑150 (MQ=255)
                                                                                                                     aaaCCATAGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAg                              >  2:3195350/1‑150 (MQ=255)
                                                                                                                            aGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTAc                       >  1:2099874/1‑150 (MQ=255)
                                                                                                                            aGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTAc                       >  1:1426131/1‑150 (MQ=255)
                                                                                                                            aGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTAc                       >  1:8350/1‑150 (MQ=255)
                                                                                                                            aGCCATATTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTAc                       >  2:235335/1‑150 (MQ=255)
                                                                                                                                aTGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGAtc                   >  2:636986/1‑150 (MQ=255)
                                                                                                                                  gTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGAtctc                 <  2:1275994/150‑1 (MQ=255)
                                                                                                                                  gTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGAtctc                 <  1:531953/150‑1 (MQ=255)
                                                                                                                                  gTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGAtctc                 <  1:1762406/150‑1 (MQ=255)
                                                                                                                                  gTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGAtctc                 <  1:341603/150‑1 (MQ=255)
                                                                                                                                   ttAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCg                <  1:665095/150‑1 (MQ=255)
                                                                                                                                   ttAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCg                <  2:2003577/150‑1 (MQ=255)
                                                                                                                                         ttttGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGc          >  2:4230743/1‑150 (MQ=255)
                                                                                                                                               ggAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTGTc    >  2:1614993/1‑150 (MQ=255)
                                                                                                                                                 aaTGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTGTCAg  >  1:2828924/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTGTCAG  >  CP009273/837568‑837862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A