Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F218 I0 R1	1506	278.7	9275112	94.5%	8764980	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,597,517	T→G	43.3%	L81* (TTA→TGA)	lsrC →	autoinducer 2 import system permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,597,517	0	T	G	43.3%	7.6 / inf	231	L81* (TTA→TGA)	lsrC	autoinducer 2 import system permease protein
Reads supporting (aligned to +/- strand): ref base T (67/64); new base G (47/53); total (114/117)
Fisher's exact test for biased strand distribution p-value = 5.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

Only 100 of 231 total aligned reads displayed.

TAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCACCCTTGGCACGTTAGGGTTGTACAGAGG  >  CP009273/1597370‑1597664

 aaGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACt                                                                                                                                                  <  1:362004/150‑1 (MQ=255)

 aaGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACt                                                                                                                                                  <  2:754434/150‑1 (MQ=255)

   gtgtGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGa                                                                                                                                                >  2:4283551/1‑150 (MQ=255)

    tgtgCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGaa                                                                                                                                               <  1:4106521/150‑1 (MQ=255)

     gtgCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAAc                                                                                                                                              <  2:3700763/150‑1 (MQ=255)

         aaaCGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAg                                                                                                                                          >  2:1930409/1‑150 (MQ=255)

         aaaCGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAg                                                                                                                                          <  2:946043/150‑1 (MQ=255)

         aaaCGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAg                                                                                                                                          <  2:1295885/150‑1 (MQ=255)

          aaCGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAgg                                                                                                                                         <  1:3081693/150‑1 (MQ=255)

           aCGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGa                                                                                                                                        >  1:965515/1‑150 (MQ=255)

            cGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACTCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGat                                                                                                                                       >  2:1079666/1‑150 (MQ=255)

              cTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGatat                                                                                                                                     >  1:1477874/1‑150 (MQ=255)

               tGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATAtt                                                                                                                                    >  1:1401429/1‑150 (MQ=255)

               tGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATAtt                                                                                                                                    <  1:4107901/150‑1 (MQ=255)

                gACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTc                                                                                                                                   >  1:3283873/1‑150 (MQ=255)

                gACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTc                                                                                                                                   <  1:1085441/150‑1 (MQ=255)

                 aCCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGAGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGTAATGTGCGCGGTGCTGTTGGGGATTTGACTGAACGCAGGATATTCa                                                                                                                                  >  2:3450153/1‑150 (MQ=255)

                  ccATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCAc                                                                                                                                 <  1:2123711/150‑1 (MQ=255)

                  ccATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCAc                                                                                                                                 >  1:2479289/1‑150 (MQ=255)

                     tGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTAc                                                                                                                              >  1:1232650/1‑150 (MQ=255)

                        tttATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTg                                                                                                                           >  1:1141724/1‑150 (MQ=255)

                          tataGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGtt                                                                                                                         <  1:1523697/150‑1 (MQ=255)

                             agcagcGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGcg                                                                                                                      >  1:897004/1‑149 (MQ=255)

                                agcGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGACTGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTtgt                                                                                                                   >  1:2001213/1‑150 (MQ=255)

                                  cgcgcAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTgtgt                                                                                                                 <  1:4537548/150‑1 (MQ=255)

                                   gcgcAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTc                                                                                                                <  2:957350/150‑1 (MQ=255)

                                    cgcAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTcg                                                                                                               <  1:3663961/150‑1 (MQ=255)

                                     gcAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTcgc                                                                                                              >  2:1449774/1‑150 (MQ=255)

                                      cAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTcgcg                                                                                                             >  1:1720982/1‑150 (MQ=255)

                                       aaaTCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGa                                                                                                            >  1:717738/1‑150 (MQ=255)

                                         atcctgatcctgCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACt                                                                                                          <  2:4361681/150‑1 (MQ=255)

                                         atcctgatcctgCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACt                                                                                                          <  1:4255632/150‑1 (MQ=255)

                                         atcctgatcctgCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACt                                                                                                          <  2:2064599/150‑1 (MQ=255)

                                          tcctgatcctgCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGACTGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACtt                                                                                                         >  2:3508747/1‑150 (MQ=255)

                                          tcctgatcctgCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACtt                                                                                                         <  2:943749/150‑1 (MQ=255)

                                           cctgatcctgCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACttt                                                                                                        <  1:3659577/150‑1 (MQ=255)

                                           cctgatcctgCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACttt                                                                                                        >  1:1184765/1‑150 (MQ=255)

                                           cctgatcctgCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACttt                                                                                                        >  1:2041804/1‑150 (MQ=255)

                                             tgatcctgCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTAc                                                                                                      >  1:534132/1‑150 (MQ=255)

                                              gatcctgCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACt                                                                                                     <  1:265874/150‑1 (MQ=255)

                                                 cctgCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCt                                                                                                  >  1:1732015/1‑150 (MQ=255)

                                                  gtgctgGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCtt                                                                                                 <  1:4474797/149‑1 (MQ=255)

                                                   tgctgGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTg                                                                                                >  1:407854/1‑150 (MQ=255)

                                                       ggCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGttt                                                                                            <  2:3097289/150‑1 (MQ=255)

                                                        gCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTg                                                                                           >  1:4594062/1‑150 (MQ=255)

                                                           aTGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTc                                                                                        >  2:3985252/1‑150 (MQ=255)

                                                              ggCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTcgcg                                                                                     <  2:1527169/150‑1 (MQ=255)

                                                                 gcgACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGa                                                                                  <  2:3208745/150‑1 (MQ=255)

                                                                   gACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGAtt                                                                                >  2:1008887/1‑150 (MQ=255)

                                                                    aCGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGAttt                                                                               >  1:4416408/1‑150 (MQ=255)

                                                                    aCGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGAttt                                                                               >  1:4269715/1‑150 (MQ=255)

                                                                      gCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGAttttt                                                                             >  2:2643207/1‑150 (MQ=255)

                                                                        tGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCa                                                                           <  2:3283873/150‑1 (MQ=255)

                                                                          gTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAAc                                                                         >  2:633403/1‑150 (MQ=255)

                                                                               gCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGgtgt                                                                    >  2:1621470/1‑150 (MQ=255)

                                                                                cTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGATGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGAATATACTGATTGGTTTGCTCGCGGGATTTTTCAACGGTGTc                                                                   >  2:2299032/1‑150 (MQ=255)

                                                                                  tACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCt                                                                 <  2:50872/150‑1 (MQ=255)

                                                                                    cgcgCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTgg                                                               >  2:2674656/1‑150 (MQ=255)

                                                                                    cgcgCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTgg                                                               <  2:2071012/150‑1 (MQ=255)

                                                                                     gcgcAATATTGATGTTTCAGTGGGTTCGATTACCGGATTGTGCTCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGt                                                              <  2:3921040/150‑1 (MQ=255)

                                                                                     gcgcAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGt                                                              <  2:3171882/150‑1 (MQ=255)

                                                                                               gttGTTTCAGTGGGTTAGATTTCCGGTATGTGCGCGGTGCTGTTGGGGATGTTACTGTACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGTGATTTTTCAACGGTGTCCTGGTCGCGTGGCTa                                                    <  2:4600235/148‑1 (MQ=255)

                                                                                                aTGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTaa                                                   <  1:1870875/150‑1 (MQ=255)

                                                                                                aTGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTaa                                                   >  1:487386/1‑150 (MQ=255)

                                                                                                 tGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTaaa                                                  <  1:778214/150‑1 (MQ=255)

                                                                                                  gTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAg                                                 <  1:823945/150‑1 (MQ=255)

                                                                                                  gTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAg                                                 >  1:1685522/1‑150 (MQ=255)

                                                                                                    ttCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGAt                                               <  2:3187886/150‑1 (MQ=255)

                                                                                                      cAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTATACTGCTTGGTTAGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATcc                                             >  2:3082234/1‑150 (MQ=255)

                                                                                                        gTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCt                                           <  1:2451573/150‑1 (MQ=255)

                                                                                                              tCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCAtt                                     <  2:2180658/150‑1 (MQ=255)

                                                                                                               cGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCAttg                                    <  2:3872493/150‑1 (MQ=255)

                                                                                                                gATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCAttgt                                   >  1:3067504/1‑150 (MQ=255)

                                                                                                                 aTTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCAttgtt                                  >  2:4233151/1‑150 (MQ=255)

                                                                                                                 aTTACCGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCAttgtt                                  <  2:1489504/150‑1 (MQ=255)

                                                                                                                    aCCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGcc                               >  2:4335434/1‑150 (MQ=255)

                                                                                                                    aCCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGcc                               >  2:3416949/1‑150 (MQ=255)

                                                                                                                     ccGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCa                              >  1:3205047/1‑150 (MQ=255)

                                                                                                                     ccGGAATGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCa                              <  1:756227/150‑1 (MQ=255)

                                                                                                                      cGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCAc                             >  1:2631600/1‑150 (MQ=255)

                                                                                                                       ggAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCAcc                            >  2:2778539/1‑150 (MQ=255)

                                                                                                                       ggAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCAcc                            >  2:2327667/1‑150 (MQ=255)

                                                                                                                          aTGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCACCCtt                         >  2:657976/1‑150 (MQ=255)

                                                                                                                          aTGTGCGCGGTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCACCCtt                         <  1:901210/150‑1 (MQ=255)

                                                                                                                            gtgCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCACCCTTgg                       <  2:391185/150‑1 (MQ=255)

                                                                                                                              gcgcGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTANAGATCCCTGCCATTGTTGCCACCCTTGGCa                     <  1:4283536/150‑1 (MQ=255)

                                                                                                                              gcgcGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCACCCTTGGCa                     <  2:1070620/150‑1 (MQ=255)

                                                                                                                                 cgGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCACCCTTGGCACGt                  <  2:1665212/150‑1 (MQ=255)

                                                                                                                                  ggTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCACCCTTGGCACGtt                 >  2:3830278/1‑150 (MQ=255)

                                                                                                                                  ggTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCACCCTTGGCACGtt                 <  1:2729401/150‑1 (MQ=255)

                                                                                                                                  ggTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCACCCTTGGCACGtt                 <  1:3825199/150‑1 (MQ=255)

                                                                                                                                  ggTGCTGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCACCCTTGGCACGtt                 <  2:3551853/150‑1 (MQ=255)

                                                                                                                                       tGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCACCCTTGGCACGTTAGGGt            <  2:2612232/150‑1 (MQ=255)

                                                                                                                                       tGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCACCCTTGGCACGTTAGGGt            <  2:39379/150‑1 (MQ=255)

                                                                                                                                       tGTTGGGGATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCACCCTTGGCACGTTAGGGt            <  2:3539749/150‑1 (MQ=255)

                                                                                                                                        gTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCACCCTTGGCACGTTAGGGtt           <  1:2932105/150‑1 (MQ=255)

                                                                                                                                           ggggATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCACCCTTGGCACGTTAGGGTTGTa        <  2:2273844/150‑1 (MQ=255)

                                                                                                                                           ggggATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCACCCTTGGCACGTTAGGGTTGTa        >  1:3318681/1‑150 (MQ=255)

                                                                                                                                            gggATGTGACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCACCCTTGGCACGTTAGGGTTGTAc       >  2:4486892/1‑150 (MQ=255)

                                                                                                                                                 gTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCACCCTTGGCACGTTAGGGTTGTACAGAgg  >  1:2524221/1‑150 (MQ=255)

TAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAATGTGCGCGGTGCTGTTGGGGATGTTACTGAACGCAGGATATTCACTACCTGTTGCTTGTGTCGCGACTTTACTGCTTGGTTTGCTCGCGGGATTTTTCAACGGTGTCCTGGTCGCGTGGCTAAAGATCCCTGCCATTGTTGCCACCCTTGGCACGTTAGGGTTGTACAGAGG  >  CP009273/1597370‑1597664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A