Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,991,843 G→A 15.4% L40F (CTC→TTC)  yecS ← ABC family putative inner membrane permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,991,8430GA15.4% 738.9 / 99.8 261L40F (CTC→TTC) yecSABC family putative inner membrane permease
Reads supporting (aligned to +/- strand):  ref base G (104/116);  new base A (23/17);  total (128/133)
Fisher's exact test for biased strand distribution p-value = 3.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 270 total aligned reads displayed.

CGGAATGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATA  >  CP009273/1991694‑1991992
                                                                                                                                                     |                                                                                                                                                     
cGGAATGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCgag                                                                                                                                                       <  2:458422/150‑1 (MQ=255)
cGGAATGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCgag                                                                                                                                                       <  2:2770881/150‑1 (MQ=255)
  gAATGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGaa                                                                                                                                                     >  1:4240090/1‑150 (MQ=255)
  gAATGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGaa                                                                                                                                                     >  1:2602461/1‑150 (MQ=255)
    aTGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATa                                                                                                                                                   >  2:1773835/1‑150 (MQ=255)
    aTGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATa                                                                                                                                                   >  1:1526471/1‑150 (MQ=255)
     tGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATaa                                                                                                                                                  >  2:2217074/1‑150 (MQ=255)
     tGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATaa                                                                                                                                                  <  2:4048121/150‑1 (MQ=255)
      gggATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATaaa                                                                                                                                                 >  2:2050268/1‑150 (MQ=255)
          tCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCg                                                                                                                                             <  1:1869388/150‑1 (MQ=255)
          tCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCg                                                                                                                                             <  1:4634839/150‑1 (MQ=255)
            tAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAg                                                                                                                                           <  1:3119342/150‑1 (MQ=255)
            tAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAg                                                                                                                                           <  1:3369772/150‑1 (MQ=255)
            tAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAg                                                                                                                                           <  1:832636/150‑1 (MQ=255)
             aaTTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGc                                                                                                                                          <  2:399080/150‑1 (MQ=255)
                   aTACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAg                                                                                                                                    >  1:3790433/1‑150 (MQ=255)
                      ccAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCa                                                                                                                                 >  2:4322725/1‑150 (MQ=255)
                       cAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCaa                                                                                                                                >  1:681605/1‑150 (MQ=255)
                        aaaCTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCaaa                                                                                                                               >  2:4485756/1‑150 (MQ=255)
                            tGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAAc                                                                                                                           >  1:1439054/1‑150 (MQ=255)
                              cGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACAt                                                                                                                         <  2:4167703/150‑1 (MQ=255)
                               ggCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATg                                                                                                                        >  1:2074543/1‑150 (MQ=255)
                                  aGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATgccg                                                                                                                     <  1:3846360/150‑1 (MQ=255)
                                   ggCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATgccgc                                                                                                                    <  2:1524150/150‑1 (MQ=255)
                                       gTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGAt                                                                                                                <  1:1026243/150‑1 (MQ=255)
                                         aaTAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATAc                                                                                                              >  2:4353941/1‑150 (MQ=255)
                                             gATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAg                                                                                                          <  1:2720384/150‑1 (MQ=255)
                                                 aTAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGc                                                                                                      <  1:3630290/150‑1 (MQ=255)
                                                          tgtgCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATAc                                                                                             <  1:2839505/150‑1 (MQ=255)
                                                          tgtgCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATAc                                                                                             <  1:2182881/150‑1 (MQ=255)
                                                          tgtgCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATAc                                                                                             <  2:3462003/150‑1 (MQ=255)
                                                            tgCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATAccc                                                                                           <  1:1260569/150‑1 (MQ=255)
                                                            tgCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATAccc                                                                                           <  1:3904488/150‑1 (MQ=255)
                                                            tgCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATAccc                                                                                           <  1:3904495/150‑1 (MQ=255)
                                                             gCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCa                                                                                          >  1:4331981/1‑150 (MQ=255)
                                                               gATGAGCGGCGTACCACGAAAAATAGATATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGc                                                                                        >  2:4366157/1‑150 (MQ=255)
                                                               gATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGc                                                                                        >  2:502482/1‑150 (MQ=255)
                                                                attaGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGcc                                                                                       >  1:2334514/4‑150 (MQ=255)
                                                                 tGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGccc                                                                                      >  2:755043/1‑150 (MQ=255)
                                                                     cggcgTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCttt                                                                                  <  1:4295046/150‑1 (MQ=255)
                                                                      ggcgTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTc                                                                                 >  1:4306386/1‑150 (MQ=255)
                                                                       gcgTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTca                                                                                <  2:3188722/150‑1 (MQ=255)
                                                                        cgTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTcag                                                                               <  1:88131/150‑1 (MQ=255)
                                                                            ccACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAgg                                                                           >  1:5044/1‑150 (MQ=255)
                                                                              aCGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGaa                                                                         <  2:1509653/150‑1 (MQ=255)
                                                                                 aaaaaTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACgg                                                                      >  1:3967920/1‑150 (MQ=255)
                                                                                  aaaaTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGc                                                                     >  2:4406863/1‑150 (MQ=255)
                                                                                  aaaaTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGACAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTACGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGACCGGc                                                                     >  2:702848/1‑150 (MQ=255)
                                                                                  aaaaTAGAGATATAAAAACGCGCAAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGc                                                                     >  1:1433068/1‑150 (MQ=255)
                                                                                   aaaTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCa                                                                    >  2:1132732/1‑150 (MQ=255)
                                                                                    aaTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCaa                                                                   <  1:1490734/150‑1 (MQ=255)
                                                                                    aaTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCaa                                                                   >  1:2404922/1‑150 (MQ=255)
                                                                                        gagaTATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGaa                                                               >  1:4544005/1‑150 (MQ=255)
                                                                                         agaTATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAAt                                                              <  2:2990354/150‑1 (MQ=255)
                                                                                         agaTATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAAt                                                              <  1:3323863/150‑1 (MQ=255)
                                                                                           atatAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGTCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCa                                                            >  2:2174814/1‑150 (MQ=255)
                                                                                            tataAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCaa                                                           >  1:96588/1‑150 (MQ=255)
                                                                                              taAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATa                                                         <  1:1729629/150‑1 (MQ=255)
                                                                                               aaaaaCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATaa                                                        <  1:4111585/150‑1 (MQ=255)
                                                                                                aaaaCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAAc                                                       <  2:292687/150‑1 (MQ=255)
                                                                                                 aaaCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAAcc                                                      >  2:2338006/1‑150 (MQ=255)
                                                                                                   aCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAg                                                    <  1:4179734/150‑1 (MQ=255)
                                                                                                    cgcgCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGACCGGCAAGGAATCAATAACCAGt                                                   >  2:339245/1‑150 (MQ=255)
                                                                                                    cgcgCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGt                                                   >  2:339339/1‑150 (MQ=255)
                                                                                                    cgcgCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGt                                                   >  1:2949554/1‑150 (MQ=255)
                                                                                                     gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGtt                                                  <  1:3517530/150‑1 (MQ=255)
                                                                                                      cgcCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTg                                                 <  1:1936821/150‑1 (MQ=255)
                                                                                                      cgcCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTg                                                 >  2:4272275/1‑150 (MQ=255)
                                                                                                        ccagccagCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGta                                               <  1:714603/150‑1 (MQ=255)
                                                                                                         cagccagcGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGtat                                              >  2:1969124/1‑150 (MQ=255)
                                                                                                         cagccagcGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGtat                                              >  1:2879055/1‑150 (MQ=255)
                                                                                                          agccagcGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGtata                                             >  1:2624539/1‑150 (MQ=255)
                                                                                                             cagcGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACtt                                          >  1:566991/1‑150 (MQ=255)
                                                                                                               gcgcACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTc                                        <  2:2855291/150‑1 (MQ=255)
                                                                                                                cgcACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCt                                       <  2:146142/150‑1 (MQ=255)
                                                                                                                cgcACGGGCCAGATGGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCt                                       <  2:1253959/150‑1 (MQ=255)
                                                                                                                   aCGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGc                                    <  2:1736912/150‑1 (MQ=255)
                                                                                                                     gggCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCAt                                  >  2:3309876/1‑150 (MQ=255)
                                                                                                                     gggCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCAt                                  >  2:1754250/1‑150 (MQ=255)
                                                                                                                     gggCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCAt                                  >  1:2668930/1‑150 (MQ=255)
                                                                                                                       gCCATATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAAAATGCCGCCGATACTGAGATGCAGCGTATACCCAGCCCCTTTCAGAAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGa                                >  2:1003846/1‑150 (MQ=255)
                                                                                                                       gCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGa                                >  1:3261231/1‑150 (MQ=255)
                                                                                                                            aTTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGtt                           <  1:1364633/150‑1 (MQ=255)
                                                                                                                               ggCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTcc                        <  2:1585536/150‑1 (MQ=255)
                                                                                                                               ggCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTcc                        <  1:2546024/150‑1 (MQ=255)
                                                                                                                                    cAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCACCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGc                   >  2:3794707/1‑150 (MQ=255)
                                                                                                                                    cAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGc                   >  1:2707195/1‑150 (MQ=255)
                                                                                                                                     aGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCt                  >  1:1048105/1‑150 (MQ=255)
                                                                                                                                     aGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCt                  <  2:2060910/150‑1 (MQ=255)
                                                                                                                                     aGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCt                  >  2:3895287/1‑150 (MQ=255)
                                                                                                                                        cGCATCAGCGCGAGAATAACCCCGAGCAGTAAGCCTAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTaaa               <  2:3828900/150‑1 (MQ=255)
                                                                                                                                         gCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAAc              >  2:1049757/1‑150 (MQ=255)
                                                                                                                                            tCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTg           <  2:4436259/150‑1 (MQ=255)
                                                                                                                                             cAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTgg          >  1:2819056/1‑150 (MQ=255)
                                                                                                                                              aGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTggg         >  1:3546258/1‑150 (MQ=255)
                                                                                                                                              aGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTggg         >  1:2266192/1‑150 (MQ=255)
                                                                                                                                              aGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTggg         <  2:2392938/150‑1 (MQ=255)
                                                                                                                                                cgcgAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGga       >  2:3021474/1‑150 (MQ=255)
                                                                                                                                                 gcgAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGgat      <  1:322106/150‑1 (MQ=255)
                                                                                                                                                     gaATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATa  <  2:2348426/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGAATGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATA  >  CP009273/1991694‑1991992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A