Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,991,843 G→A 9.2% L40F (CTC→TTC)  yecS ← ABC family putative inner membrane permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,991,8430GA 9.2% 879.5 / 55.5 260L40F (CTC→TTC) yecSABC family putative inner membrane permease
Reads supporting (aligned to +/- strand):  ref base G (113/123);  new base A (9/15);  total (122/138)
Fisher's exact test for biased strand distribution p-value = 3.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 273 total aligned reads displayed.

CGGAATGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATA  >  CP009273/1991694‑1991992
                                                                                                                                                     |                                                                                                                                                      
 ggAATGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCgaga                                                                                                                                                       >  2:3204766/1‑150 (MQ=255)
   aaTGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAAt                                                                                                                                                     <  1:2860092/150‑1 (MQ=255)
    aTGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATa                                                                                                                                                    >  1:763148/1‑150 (MQ=255)
    aTGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATa                                                                                                                                                    >  1:4154214/1‑150 (MQ=255)
     tGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATaa                                                                                                                                                   >  2:1689326/1‑150 (MQ=255)
      gggATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATaaa                                                                                                                                                  <  2:3372732/150‑1 (MQ=255)
      gggATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATaaa                                                                                                                                                  >  1:2765188/1‑150 (MQ=255)
       ggATCTACTTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAAc                                                                                                                                                 <  1:3320252/150‑1 (MQ=255)
       ggATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAAGAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAAc                                                                                                                                                 >  2:916732/1‑150 (MQ=255)
         aTCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAAccc                                                                                                                                               >  1:1072344/1‑150 (MQ=255)
         aTCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAAccc                                                                                                                                               <  1:2167632/150‑1 (MQ=255)
          tCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCg                                                                                                                                              >  1:2733024/1‑150 (MQ=255)
          tCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCg                                                                                                                                              <  1:1701232/150‑1 (MQ=255)
           cTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGa                                                                                                                                             >  2:31011/1‑150 (MQ=255)
            tAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAg                                                                                                                                            <  2:22126/150‑1 (MQ=255)
            tAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAg                                                                                                                                            <  2:1463384/150‑1 (MQ=255)
                    tACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGc                                                                                                                                    >  1:1273637/1‑150 (MQ=255)
                      ccAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑a                                                                                                                                 <  1:2206379/150‑1 (MQ=255)
                       cAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑aa                                                                                                                                >  1:3951635/1‑150 (MQ=255)
                        aaaCTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑aaa                                                                                                                               >  1:2510291/1‑150 (MQ=255)
                           cTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑aaaaaa                                                                                                                            >  1:3615014/1‑150 (MQ=255)
                              cGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACAt                                                                                                                         <  1:4107687/150‑1 (MQ=255)
                               ggCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATg                                                                                                                        >  2:1536579/1‑150 (MQ=255)
                                gCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAACCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATgc                                                                                                                       <  2:1907950/150‑1 (MQ=255)
                                gCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATgc                                                                                                                       >  2:636183/1‑150 (MQ=255)
                                 cAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATgcc                                                                                                                      >  2:3436055/1‑150 (MQ=255)
                                  aGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATgccg                                                                                                                     >  2:3629943/1‑150 (MQ=255)
                                  aGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATgccg                                                                                                                     >  1:2170496/1‑150 (MQ=255)
                                  aGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATgccg                                                                                                                     >  2:2636684/1‑150 (MQ=255)
                                   ggCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATgccgc                                                                                                                    >  1:1202381/1‑150 (MQ=255)
                                   ggCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCC‑AAAAAACATgccgc                                                                                                                    >  1:284112/1‑150 (MQ=255)
                                    gCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATgccgcc                                                                                                                   <  1:3782468/150‑1 (MQ=255)
                                         aaTAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATAc                                                                                                              <  2:2477370/150‑1 (MQ=255)
                                          aTAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGTGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACt                                                                                                             <  1:3985289/150‑1 (MQ=255)
                                           tAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTg                                                                                                            >  2:3382494/1‑150 (MQ=255)
                                            aGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGa                                                                                                           >  2:2441132/1‑150 (MQ=255)
                                             gATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAg                                                                                                          <  1:1487333/150‑1 (MQ=255)
                                                cATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTg                                                                                                       >  2:649154/1‑150 (MQ=255)
                                                cATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTg                                                                                                       >  1:136647/1‑150 (MQ=255)
                                                cATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTg                                                                                                       >  1:2007986/1‑150 (MQ=255)
                                                  tAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCa                                                                                                     <  1:3512229/150‑1 (MQ=255)
                                                  tAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCa                                                                                                     <  2:2769375/150‑1 (MQ=255)
                                                  tAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCa                                                                                                     >  2:3484646/1‑150 (MQ=255)
                                                  tAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCa                                                                                                     <  1:1419194/150‑1 (MQ=255)
                                                      cAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGt                                                                                                 <  2:3534777/150‑1 (MQ=255)
                                                       aGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGta                                                                                                <  2:3741930/150‑1 (MQ=255)
                                                       aGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGta                                                                                                <  2:327059/150‑1 (MQ=255)
                                                         ttGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGtata                                                                                              <  1:2661201/150‑1 (MQ=255)
                                                          tgtgCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATAc                                                                                             <  2:1233882/150‑1 (MQ=255)
                                                            tgCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATAccc                                                                                           >  1:1884769/1‑150 (MQ=255)
                                                            tgCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATAccc                                                                                           <  1:1798713/150‑1 (MQ=255)
                                                                 tGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGccc                                                                                      >  2:2966108/1‑150 (MQ=255)
                                                                       gcgTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTca                                                                                <  1:4119163/150‑1 (MQ=255)
                                                                        cgTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTcag                                                                               <  1:3419020/150‑1 (MQ=255)
                                                                           aCCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTcagcag                                                                            <  1:2136967/150‑1 (MQ=255)
                                                                             cACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGa                                                                          >  2:2856827/1‑150 (MQ=255)
                                                                              aCGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGaa                                                                         >  1:2491675/1‑150 (MQ=255)
                                                                               cGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAAc                                                                        <  2:1149666/150‑1 (MQ=255)
                                                                               cGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAAc                                                                        >  1:2270648/1‑150 (MQ=255)
                                                                               cGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAAc                                                                        <  1:1400484/150‑1 (MQ=255)
                                                                                gAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACg                                                                       >  1:2044798/1‑150 (MQ=255)
                                                                                gAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACg                                                                       <  1:790188/150‑1 (MQ=255)
                                                                                  aaaaTAGAGATATAACAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGc                                                                     <  2:4175586/150‑1 (MQ=255)
                                                                                   aaaTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCa                                                                    >  2:796252/1‑150 (MQ=255)
                                                                                         agaTATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAAt                                                              <  1:30021/150‑1 (MQ=255)
                                                                                         agaTATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAAt                                                              <  1:432753/150‑1 (MQ=255)
                                                                                              taAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATa                                                         <  1:1272305/150‑1 (MQ=255)
                                                                                               aaaaaCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATaa                                                        >  1:1566714/1‑150 (MQ=255)
                                                                                               aaaaaCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATaa                                                        >  2:45910/1‑150 (MQ=255)
                                                                                                  aaCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGACTCAATAACCa                                                     >  2:4128814/1‑150 (MQ=255)
                                                                                                  aaCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCa                                                     >  2:1731866/1‑150 (MQ=255)
                                                                                                  aaCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCa                                                     >  2:2373856/1‑150 (MQ=255)
                                                                                                         cagccagcGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGtat                                              >  1:3220916/1‑150 (MQ=255)
                                                                                                            ccagcGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATAc                                            >  1:3553631/1‑150 (MQ=255)
                                                                                                              agcGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACttt                                         >  2:2006091/1‑150 (MQ=255)
                                                                                                                cgcACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCt                                       >  2:1692589/1‑150 (MQ=255)
                                                                                                                 gcACGGTCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAATAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCtt                                      <  1:2459426/150‑1 (MQ=255)
                                                                                                                 gcACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCtt                                      <  2:194252/150‑1 (MQ=255)
                                                                                                                 gcACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCtt                                      <  2:3982257/150‑1 (MQ=255)
                                                                                                                  cACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTg                                     <  2:272541/150‑1 (MQ=255)
                                                                                                                   aCGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGc                                    <  1:2567153/150‑1 (MQ=255)
                                                                                                                      ggCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATg                                 >  1:927720/1‑150 (MQ=255)
                                                                                                                         cAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGc                              <  1:1007263/150‑1 (MQ=255)
                                                                                                                         cAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGc                              <  2:3183305/150‑1 (MQ=255)
                                                                                                                         cAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGc                              >  1:560803/1‑150 (MQ=255)
                                                                                                                           gATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGt                            >  1:3572400/1‑150 (MQ=255)
                                                                                                                              tGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGTCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTc                         >  2:3596003/1‑150 (MQ=255)
                                                                                                                                 cGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCgg                      <  1:4150246/150‑1 (MQ=255)
                                                                                                                                 cGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCgg                      <  2:3858607/150‑1 (MQ=255)
                                                                                                                                  gACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATACCCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGa                     <  1:638961/150‑1 (MQ=255)
                                                                                                                                  gACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGa                     <  2:4081928/150‑1 (MQ=255)
                                                                                                                                   aCAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAg                    >  2:1033713/1‑150 (MQ=255)
                                                                                                                                   aCAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAg                    <  1:1733524/150‑1 (MQ=255)
                                                                                                                                    cAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGc                   >  2:438861/1‑150 (MQ=255)
                                                                                                                                     aGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCt                  <  1:3843636/150‑1 (MQ=255)
                                                                                                                                     aGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCt                  >  1:2619784/1‑150 (MQ=255)
                                                                                                                                            tCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTg           <  2:1853889/150‑1 (MQ=255)
                                                                                                                                                cgcgAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGga       >  1:546536/1‑150 (MQ=255)
                                                                                                                                                 gcgAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGgat      <  2:1332606/150‑1 (MQ=255)
                                                                                                                                                     gaATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATa  <  1:1300247/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CGGAATGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCC‑AAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATA  >  CP009273/1991694‑1991992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A