Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,433,018 T→G 15.5% E114A (GAG→GCG)  rplQ ← 50S ribosomal subunit protein L17

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,433,0180TG15.5% 646.9 / 140.2 284E114A (GAG→GCG) rplQ50S ribosomal subunit protein L17
Reads supporting (aligned to +/- strand):  ref base T (135/105);  new base G (34/10);  total (169/115)
Fisher's exact test for biased strand distribution p-value = 1.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 285 total aligned reads displayed.

ATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGA  >  CP009273/3432871‑3433167
                                                                                                                                                   |                                                                                                                                                     
aTACTCCAGGGGATGAACAAAAAGTGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTAAGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCg                                                                                                                                                     <  2:22603/150‑1 (MQ=255)
aTACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCg                                                                                                                                                     <  2:22639/150‑1 (MQ=255)
    tCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGt                                                                                                                                                 <  2:945685/150‑1 (MQ=255)
    tCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGt                                                                                                                                                 >  1:2385282/1‑150 (MQ=255)
      cAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTaa                                                                                                                                               >  1:4528419/1‑150 (MQ=255)
       aGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAg                                                                                                                                              >  2:548063/1‑150 (MQ=255)
       aGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAg                                                                                                                                              >  2:592494/1‑150 (MQ=255)
        ggggATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGc                                                                                                                                             >  2:3969426/1‑150 (MQ=255)
          ggATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCa                                                                                                                                           <  2:2352926/150‑1 (MQ=255)
          ggATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCa                                                                                                                                           >  2:1428668/1‑150 (MQ=255)
            aTGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCGCGATGTAAGCCATc                                                                                                                                         >  2:4594057/1‑150 (MQ=255)
              gAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCgg                                                                                                                                       <  2:2208944/150‑1 (MQ=255)
              gAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCGCGATGTAAGCCATCgg                                                                                                                                       >  1:2553056/1‑150 (MQ=255)
                aCAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgcg                                                                                                                                     >  2:4167047/1‑150 (MQ=255)
                aCAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgcg                                                                                                                                     >  1:1250279/1‑150 (MQ=255)
                  aaaaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGcgcg                                                                                                                                   >  1:2676891/1‑150 (MQ=255)
                  aaaaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCGCGATGTAAGCCATCGGcgcg                                                                                                                                   <  1:162024/150‑1 (MQ=255)
                   aaaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGt                                                                                                                                  >  2:1200574/1‑150 (MQ=255)
                    aaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGtt                                                                                                                                 >  2:1725561/1‑150 (MQ=255)
                    aaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGtt                                                                                                                                 >  1:2100498/1‑150 (MQ=255)
                     aaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTg                                                                                                                                >  1:1410965/1‑150 (MQ=255)
                       gagTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTc                                                                                                                              <  2:2299335/150‑1 (MQ=255)
                           aCAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCt                                                                                                                          <  2:3925629/150‑1 (MQ=255)
                           aCAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCt                                                                                                                          <  2:2763444/150‑1 (MQ=255)
                            cAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTg                                                                                                                         <  1:3955945/150‑1 (MQ=255)
                             aGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGACCGATCAACCAGCTCGCTGGAAGCCATCGGCGCGTTGTCGCCTGc                                                                                                                        >  2:2971170/1‑150 (MQ=255)
                             aGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTACGCCATCGGCGCGTTGTCGCCTGc                                                                                                                        >  2:656961/1‑150 (MQ=255)
                               cTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCAc                                                                                                                      <  1:1325033/150‑1 (MQ=255)
                                  ttGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGa                                                                                                                   <  2:1609381/150‑1 (MQ=255)
                                    gTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAg                                                                                                                 >  1:3337881/1‑150 (MQ=255)
                                     tAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCGCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGc                                                                                                                >  2:70683/1‑150 (MQ=255)
                                      aGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCGCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGcc                                                                                                               >  1:31901/1‑150 (MQ=255)
                                       gATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCca                                                                                                              >  1:3774651/1‑150 (MQ=255)
                                         tAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCcaca                                                                                                            >  1:1480565/1‑150 (MQ=255)
                                         tAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCcaca                                                                                                            <  1:1927661/150‑1 (MQ=255)
                                           aGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGTACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACCCt                                                                                                          >  2:3255464/1‑150 (MQ=255)
                                            gTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACtt                                                                                                         <  1:2704077/150‑1 (MQ=255)
                                                ggATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGa                                                                                                     <  1:1758436/150‑1 (MQ=255)
                                                   tactacGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATa                                                                                                  >  2:308359/1‑150 (MQ=255)
                                                    actacGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCGCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATAc                                                                                                 <  2:2669455/150‑1 (MQ=255)
                                                     ctacGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACg                                                                                                <  1:3562873/150‑1 (MQ=255)
                                                     ctacGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCGTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCGCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACg                                                                                                >  1:3420248/1‑150 (MQ=255)
                                                         gggTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAgtg                                                                                            >  2:2516899/1‑150 (MQ=255)
                                                          ggTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAgtgt                                                                                           <  1:4178196/150‑1 (MQ=255)
                                                            tataAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTaa                                                                                         >  1:2840040/1‑150 (MQ=255)
                                                             ataAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAac                                                                                        >  1:3713945/1‑150 (MQ=255)
                                                               aaaaaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTAATCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCGCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACGTCAGAATACGAGTGTAacca                                                                                      >  1:999040/1‑150 (MQ=255)
                                                                aaaaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTGGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAaccac                                                                                     >  1:547867/1‑150 (MQ=255)
                                                                    aaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCCCCGGc                                                                                 >  2:4520609/1‑150 (MQ=255)
                                                                      cccGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCAc                                                                               <  2:726024/150‑1 (MQ=255)
                                                                       ccgccgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACg                                                                              <  1:1516368/150‑1 (MQ=255)
                                                                        cgccgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACgg                                                                             >  2:3924022/1‑150 (MQ=255)
                                                                          ccgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGAAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCt                                                                           <  1:862822/150‑1 (MQ=255)
                                                                           cgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTc                                                                          >  2:3047086/1‑150 (MQ=255)
                                                                           cgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTc                                                                          <  1:2206505/150‑1 (MQ=255)
                                                                           cgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTc                                                                          >  2:384909/1‑150 (MQ=255)
                                                                           cgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCGCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTc                                                                          >  1:4233058/1‑150 (MQ=255)
                                                                            ggggCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTcg                                                                         >  1:3592711/1‑150 (MQ=255)
                                                                               gCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCGCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGa                                                                      >  2:206505/1‑150 (MQ=255)
                                                                                cGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGaa                                                                     <  1:1002866/150‑1 (MQ=255)
                                                                                 gggTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGaaa                                                                    <  2:4283546/150‑1 (MQ=255)
                                                                                       ttttACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCggg                                                              <  1:4346882/150‑1 (MQ=255)
                                                                                           aCGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCa                                                          <  2:4450419/150‑1 (MQ=255)
                                                                                             gTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGt                                                        >  1:532917/1‑150 (MQ=255)
                                                                                             gTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCGCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGt                                                        <  1:1039271/150‑1 (MQ=255)
                                                                                                gCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCg                                                     >  1:799687/1‑150 (MQ=255)
                                                                                                 cTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGt                                                    >  1:3803579/1‑150 (MQ=255)
                                                                                                   tCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTa                                                  >  1:665117/1‑150 (MQ=255)
                                                                                                      gATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAAc                                               <  2:1177129/150‑1 (MQ=255)
                                                                                                       aTTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACa                                              <  1:1679612/150‑1 (MQ=255)
                                                                                                       aTTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACa                                              >  1:622791/1‑150 (MQ=255)
                                                                                                       aTTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACa                                              >  2:3666211/1‑150 (MQ=255)
                                                                                                        ttACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCGCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAg                                             >  1:1938220/1‑150 (MQ=255)
                                                                                                           ctctGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGttt                                          >  1:4047871/1‑150 (MQ=255)
                                                                                                            tctGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGtttt                                         <  2:3732881/150‑1 (MQ=255)
                                                                                                             ctGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTg                                        >  2:362320/1‑150 (MQ=255)
                                                                                                                cagcagCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCa                                     >  2:342846/1‑150 (MQ=255)
                                                                                                                 agcagcTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCAc                                    >  2:1769229/1‑150 (MQ=255)
                                                                                                                   cagcTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGa                                  >  2:3407448/1‑150 (MQ=255)
                                                                                                                   cagcTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGa                                  >  2:1553662/1‑150 (MQ=255)
                                                                                                                    agcTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGAt                                 <  1:2261830/150‑1 (MQ=255)
                                                                                                                       ttCTGCTTTCTCTGAACGATCAACCAGCGCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGAtctc                              >  2:84982/1‑150 (MQ=255)
                                                                                                                        tCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCg                             >  2:742462/1‑150 (MQ=255)
                                                                                                                        tCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCg                             <  2:2870527/150‑1 (MQ=255)
                                                                                                                         cTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGt                            <  1:3851720/150‑1 (MQ=255)
                                                                                                                          tGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGtt                           >  2:3368979/1‑150 (MQ=255)
                                                                                                                                ctctGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACg                     <  1:2005257/150‑1 (MQ=255)
                                                                                                                                ctctGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACg                     >  2:896158/1‑150 (MQ=255)
                                                                                                                                ctctGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACg                     <  2:3776102/150‑1 (MQ=255)
                                                                                                                                    gAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTa                 >  1:4151990/1‑150 (MQ=255)
                                                                                                                                     aaCGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTAc                >  1:4107781/1‑150 (MQ=255)
                                                                                                                                      aCGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACg               <  2:4396765/150‑1 (MQ=255)
                                                                                                                                       cGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACgg              >  2:2102966/1‑150 (MQ=255)
                                                                                                                                         aTCAACCAGCGCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGc            >  1:481450/1‑150 (MQ=255)
                                                                                                                                           cAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGa          >  1:486961/1‑150 (MQ=255)
                                                                                                                                            aaCCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGaa         <  2:3550980/150‑1 (MQ=255)
                                                                                                                                            aaCCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGaa         >  1:1520827/1‑150 (MQ=255)
                                                                                                                                             aCCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAAt        <  1:4421773/150‑1 (MQ=255)
                                                                                                                                                 gCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCa    <  2:699786/150‑1 (MQ=255)
                                                                                                                                                 gCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCa    >  2:1330122/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGA  >  CP009273/3432871‑3433167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A