Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,816,686 G→T 22.5% S309S (TCG→TCT)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,816,6860GT22.5% 721.7 / 148.7 273S309S (TCG→TCT) spoTbifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (100/111);  new base T (26/36);  total (126/147)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

ATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGAT  >  CP009273/3816537‑3816835
                                                                                                                                                     |                                                                                                                                                     
aTCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCg                                                                                                                                                       >  1:954359/1‑150 (MQ=255)
  cTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCgat                                                                                                                                                     >  2:1562149/1‑150 (MQ=255)
   tACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCgatg                                                                                                                                                    <  2:159453/150‑1 (MQ=255)
        tttCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCgg                                                                                                                                               <  2:2306939/150‑1 (MQ=255)
         ttCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGc                                                                                                                                              <  1:2607743/150‑1 (MQ=255)
           ccGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGccc                                                                                                                                            <  1:3386110/150‑1 (MQ=255)
           ccGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTAGATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGccc                                                                                                                                            <  2:2841549/150‑1 (MQ=255)
            cgcgTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCg                                                                                                                                           <  2:4364002/150‑1 (MQ=255)
                 gATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACgg                                                                                                                                      >  1:884179/1‑150 (MQ=255)
                 gATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACgg                                                                                                                                      >  1:2315276/1‑150 (MQ=255)
                     gTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGtgtg                                                                                                                                  <  2:3060852/150‑1 (MQ=255)
                     gTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGtgtg                                                                                                                                  <  2:129034/150‑1 (MQ=255)
                        aaTGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCg                                                                                                                               >  1:1760341/1‑150 (MQ=255)
                         aTGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCgg                                                                                                                              >  2:4475117/1‑150 (MQ=255)
                         aTGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCgg                                                                                                                              >  1:3512983/1‑150 (MQ=255)
                         aTGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCgg                                                                                                                              <  2:1124776/150‑1 (MQ=255)
                          tGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGt                                                                                                                             >  1:4418152/1‑150 (MQ=255)
                          tGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGt                                                                                                                             <  1:1550270/150‑1 (MQ=255)
                           gATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGtt                                                                                                                            >  1:2703541/1‑150 (MQ=255)
                           gATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGtt                                                                                                                            >  2:1731264/1‑150 (MQ=255)
                              tCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAg                                                                                                                         <  2:764331/150‑1 (MQ=255)
                               cTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAgg                                                                                                                        >  1:3162984/1‑150 (MQ=255)
                               cTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAgg                                                                                                                        >  1:1656956/1‑150 (MQ=255)
                                tGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGTGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGt                                                                                                                       >  1:812250/1‑150 (MQ=255)
                                   cacCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCa                                                                                                                    >  1:4265724/1‑150 (MQ=255)
                                    acCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTCTATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAg                                                                                                                   >  2:3558693/1‑150 (MQ=255)
                                    acCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAg                                                                                                                   <  2:4438328/150‑1 (MQ=255)
                                     ccTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGa                                                                                                                  >  1:1869759/1‑150 (MQ=255)
                                       tGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATc                                                                                                                >  1:3934031/1‑150 (MQ=255)
                                       tGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATc                                                                                                                >  2:3772767/1‑150 (MQ=255)
                                        gTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATcc                                                                                                               <  1:2045597/150‑1 (MQ=255)
                                         ttATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCg                                                                                                              <  2:1800797/150‑1 (MQ=255)
                                          tATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGt                                                                                                             >  1:4192677/1‑150 (MQ=255)
                                          tATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGt                                                                                                             <  1:3617776/150‑1 (MQ=255)
                                                gTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGaa                                                                                                       >  2:2514007/1‑150 (MQ=255)
                                                   cTGGGCCAGAGGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTCTATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGat                                                                                                    <  2:2777267/150‑1 (MQ=255)
                                                     gggCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGatat                                                                                                  <  1:1393704/150‑1 (MQ=255)
                                                          aGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGAcc                                                                                             <  1:3015610/150‑1 (MQ=255)
                                                             tGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGa                                                                                          >  2:3625489/1‑150 (MQ=255)
                                                              gctcaGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGAt                                                                                         >  1:2484655/4‑150 (MQ=255)
                                                                tcaGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATgg                                                                                       >  1:3308490/2‑150 (MQ=255)
                                                                tcaGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATgg                                                                                       >  2:3360133/2‑150 (MQ=255)
                                                                    ccTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGga                                                                                   <  1:1123773/150‑1 (MQ=255)
                                                                        tACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATg                                                                               <  2:273467/150‑1 (MQ=255)
                                                                         aCAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATgg                                                                              >  1:3181414/1‑150 (MQ=255)
                                                                           aaGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGgt                                                                            >  2:3902731/1‑150 (MQ=255)
                                                                            aGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGgtg                                                                           <  1:4473349/150‑1 (MQ=255)
                                                                            aGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGgtg                                                                           >  1:1039044/1‑150 (MQ=255)
                                                                            aGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGgtg                                                                           >  2:212591/1‑150 (MQ=255)
                                                                              ccGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGtt                                                                         <  2:2103531/150‑1 (MQ=255)
                                                                               cgcgTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAACGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTg                                                                        <  2:1848372/150‑1 (MQ=255)
                                                                               cgcgTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTg                                                                        <  1:2739097/150‑1 (MQ=255)
                                                                               cgcgTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTg                                                                        <  1:2451864/150‑1 (MQ=255)
                                                                                gcgTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGc                                                                       >  1:632107/1‑150 (MQ=255)
                                                                                 cgTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGcc                                                                      <  1:1476787/150‑1 (MQ=255)
                                                                                  gTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCcg                                                                     <  2:687059/150‑1 (MQ=255)
                                                                                  gTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGTCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCcg                                                                     <  1:3371557/150‑1 (MQ=255)
                                                                                         ccGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTg                                                              <  2:3273528/150‑1 (MQ=255)
                                                                                         ccGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTg                                                              <  2:1487009/150‑1 (MQ=255)
                                                                                          cgcgTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTgg                                                             <  2:4039008/150‑1 (MQ=255)
                                                                                            cgTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGc                                                           >  1:1968989/1‑150 (MQ=255)
                                                                                            cgTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCATTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGc                                                           <  1:1062984/150‑1 (MQ=255)
                                                                                              tGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCtt                                                         <  1:206751/150‑1 (MQ=255)
                                                                                                 aaGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTtata                                                      >  2:1330266/1‑150 (MQ=255)
                                                                                                     cTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAaga                                                  <  1:2996959/150‑1 (MQ=255)
                                                                                                      tataTCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAagag                                                 >  1:1150301/1‑150 (MQ=255)
                                                                                                       atatCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGc                                                <  2:2425276/150‑1 (MQ=255)
                                                                                                        tatCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCa                                               <  1:1267036/150‑1 (MQ=255)
                                                                                                         atCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCAc                                              <  2:1470994/150‑1 (MQ=255)
                                                                                                         atCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCAc                                              >  1:2934589/1‑150 (MQ=255)
                                                                                                           cGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACgg                                            <  2:497298/150‑1 (MQ=255)
                                                                                                              cATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGa                                         >  2:906850/1‑150 (MQ=255)
                                                                                                              cATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGa                                         <  2:543995/150‑1 (MQ=255)
                                                                                                              cATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGa                                         <  1:2171695/150‑1 (MQ=255)
                                                                                                              cATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGa                                         >  2:3972857/1‑150 (MQ=255)
                                                                                                                ttCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGaaa                                       >  2:2272674/1‑150 (MQ=255)
                                                                                                                 tCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAAc                                      >  2:2597821/1‑150 (MQ=255)
                                                                                                                  ccAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAAcc                                     <  2:3183394/150‑1 (MQ=255)
                                                                                                                  ccAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAAcc                                     >  2:3414748/1‑150 (MQ=255)
                                                                                                                   cAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCa                                    >  2:1984581/1‑150 (MQ=255)
                                                                                                                   cAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCa                                    <  2:2160847/150‑1 (MQ=255)
                                                                                                                   cAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCa                                    >  2:4291762/1‑150 (MQ=255)
                                                                                                                    atcaGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCTCGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGAGCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAg                                   <  2:4183154/147‑1 (MQ=255)
                                                                                                                    aaaaGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAg                                   <  1:4197470/150‑1 (MQ=255)
                                                                                                                    aaaaGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAg                                   >  1:3945283/1‑150 (MQ=255)
                                                                                                                     aaaGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGt                                  <  1:66355/150‑1 (MQ=255)
                                                                                                                      aaGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGta                                 >  1:2616482/1‑150 (MQ=255)
                                                                                                                         cGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGtacta                              <  1:1555859/150‑1 (MQ=255)
                                                                                                                          gAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGtactac                             >  1:2217772/1‑150 (MQ=255)
                                                                                                                           aaCGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTAcc                            >  1:2973547/1‑150 (MQ=255)
                                                                                                                           aaCGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTAcc                            >  2:4498079/1‑150 (MQ=255)
                                                                                                                            aCGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCg                           <  1:701047/150‑1 (MQ=255)
                                                                                                                                    cAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATc                   >  2:38001/1‑150 (MQ=255)
                                                                                                                                     aGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATcc                  <  2:2691398/150‑1 (MQ=255)
                                                                                                                                      gTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCcg                 <  2:293916/150‑1 (MQ=255)
                                                                                                                                        cTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCcgcg               <  1:3670975/150‑1 (MQ=255)
                                                                                                                                             cacaCCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAg          >  1:2896088/1‑150 (MQ=255)
                                                                                                                                                 ccTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCt      <  1:4608980/150‑1 (MQ=255)
                                                                                                                                                 ccTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCt      <  2:3162984/150‑1 (MQ=255)
                                                                                                                                                     gatgatCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGAt  <  1:3238012/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGAT  >  CP009273/3816537‑3816835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A