Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,011,929 T→A 100% intergenic (‑3/+66) fabA ← / ← ycbZ beta‑hydroxydecanoyl thioester dehydrase/putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,011,9290TA97.6% 681.0 / 4.5 208intergenic (‑3/+66)fabA/ycbZbeta‑hydroxydecanoyl thioester dehydrase/putative peptidase
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base A (84/119);  total (86/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 212 total aligned reads displayed.

CCACCCGTTTCGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTTCTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCG  >  CP009273/1011784‑1012073
                                                                                                                                                 |                                                                                                                                                
ccaccCGTTTCGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTTTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTact                                                                                                                                                <  2:1151678/148‑1 (MQ=255)
ccaccCGTTTCGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTact                                                                                                                                                >  2:3063622/1‑148 (MQ=255)
  accCGTTTCGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTActct                                                                                                                                              <  1:3125775/148‑1 (MQ=255)
   cccGTTTCGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTg                                                                                                                                             >  1:3289821/1‑148 (MQ=255)
    ccGTTTCGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGt                                                                                                                                            <  2:3767029/148‑1 (MQ=255)
        ttCGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGc                                                                                                                                        <  2:2107116/148‑1 (MQ=255)
            gTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCtta                                                                                                                                    >  1:282005/1‑148 (MQ=255)
              cATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCttatt                                                                                                                                  >  2:3119856/1‑148 (MQ=255)
                 tttGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCttatttta                                                                                                                               <  1:974778/148‑1 (MQ=255)
                 tttGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCttatttta                                                                                                                               >  2:1450930/1‑148 (MQ=255)
                 tttGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCttatttta                                                                                                                               <  1:2771386/148‑1 (MQ=255)
                  ttGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCttattttat                                                                                                                              <  2:101539/148‑1 (MQ=255)
                     aCCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGa                                                                                                                           >  2:1530536/1‑148 (MQ=255)
                       cacaCGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAg                                                                                                                         <  1:452262/148‑1 (MQ=255)
                        acacGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGc                                                                                                                        <  1:2910419/148‑1 (MQ=255)
                         cacGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCa                                                                                                                       <  1:1446447/148‑1 (MQ=255)
                         cacGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCa                                                                                                                       <  1:2117227/148‑1 (MQ=255)
                           cGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACg                                                                                                                     >  2:2259632/1‑148 (MQ=255)
                               ccATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTGAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAgg                                                                                                                 <  2:709744/148‑1 (MQ=255)
                                catcatCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGa                                                                                                                >  2:1764314/1‑148 (MQ=255)
                                catcatCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGAGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGa                                                                                                                <  1:3270450/148‑1 (MQ=255)
                                 atcatcAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGAt                                                                                                               >  1:2545048/1‑148 (MQ=255)
                                 atcatcAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGAt                                                                                                               >  1:2191792/1‑148 (MQ=255)
                                  tcatcaGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATa                                                                                                              <  1:3085652/148‑1 (MQ=255)
                                  tcatcaGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATa                                                                                                              <  2:3447274/148‑1 (MQ=255)
                                   catcaGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAg                                                                                                             <  1:1232695/148‑1 (MQ=255)
                                    atcaGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGc                                                                                                            <  2:1236301/148‑1 (MQ=255)
                                    atcaGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGc                                                                                                            <  2:1361009/148‑1 (MQ=255)
                                     tcaGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCt                                                                                                           >  1:634363/1‑148 (MQ=255)
                                      caGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTa                                                                                                          >  1:103480/1‑148 (MQ=255)
                                      caGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTa                                                                                                          >  1:3513626/1‑148 (MQ=255)
                                      caGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTa                                                                                                          >  2:1252999/1‑148 (MQ=255)
                                       aGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTaa                                                                                                         <  2:1766984/148‑1 (MQ=255)
                                         cATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAaca                                                                                                       >  1:3641474/1‑148 (MQ=255)
                                            gTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACgt                                                                                                    >  1:2006462/1‑148 (MQ=255)
                                              tCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACgtgt                                                                                                  <  1:3819641/148‑1 (MQ=255)
                                              tCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACgtgt                                                                                                  <  2:2485680/148‑1 (MQ=255)
                                                 gTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTTCTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACg                                                                                               >  1:154121/1‑148 (MQ=255)
                                                    cTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTg                                                                                            >  2:372155/1‑148 (MQ=255)
                                                    cTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTg                                                                                            <  1:1796384/148‑1 (MQ=255)
                                                       gCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAAc                                                                                         <  2:234443/148‑1 (MQ=255)
                                                         aaTTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACaa                                                                                       <  1:3750039/148‑1 (MQ=255)
                                                          aTTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAg                                                                                      >  1:2938634/1‑148 (MQ=255)
                                                          aTTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAg                                                                                      <  1:943299/148‑1 (MQ=255)
                                                            tGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTTCTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTc                                                                                    <  2:2574448/148‑1 (MQ=255)
                                                                ggCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGAt                                                                                <  2:515758/148‑1 (MQ=255)
                                                                 gCCTTGAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATc                                                                               <  2:3019042/148‑1 (MQ=255)
                                                                  ccTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCa                                                                              >  1:789161/1‑148 (MQ=255)
                                                                    tttCGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCGACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGt                                                                            <  2:1729238/148‑1 (MQ=255)
                                                                    tttAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGt                                                                            <  1:2349888/148‑1 (MQ=255)
                                                                    tttAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGt                                                                            <  1:3307432/148‑1 (MQ=255)
                                                                    tttAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGt                                                                            <  2:3185008/148‑1 (MQ=255)
                                                                     ttAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGtt                                                                           >  1:1091136/1‑148 (MQ=255)
                                                                        gcgcCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCgg                                                                        >  1:1630224/1‑148 (MQ=255)
                                                                         cgcCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGa                                                                       <  1:1456228/148‑1 (MQ=255)
                                                                         cgcCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGa                                                                       >  1:1827114/1‑148 (MQ=255)
                                                                          gcCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGaa                                                                      <  2:3354189/148‑1 (MQ=255)
                                                                          gcCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGaa                                                                      <  2:1567389/148‑1 (MQ=255)
                                                                            cAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATa                                                                    <  2:2443136/148‑1 (MQ=255)
                                                                              aaCAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATaaa                                                                  >  1:56100/1‑148 (MQ=255)
                                                                              aaCAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATaaa                                                                  <  1:2360861/148‑1 (MQ=255)
                                                                                  gTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAg                                                              <  2:736891/148‑1 (MQ=255)
                                                                                   ttCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGt                                                             <  1:3052214/148‑1 (MQ=255)
                                                                                        cgcgACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGc                                                        >  2:3296615/1‑148 (MQ=255)
                                                                                             ccAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGGTCGGAATAAACCAGTTCAGCCAACg                                                   >  1:2158523/1‑148 (MQ=255)
                                                                                               agagGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTa                                                 <  2:282017/148‑1 (MQ=255)
                                                                                               agagGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTa                                                 <  2:2493432/148‑1 (MQ=255)
                                                                                                 agGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAAt                                               <  1:2947253/148‑1 (MQ=255)
                                                                                                    cAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGc                                            >  2:289389/1‑148 (MQ=255)
                                                                                                      agaagGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCa                                          <  1:2612188/148‑1 (MQ=255)
                                                                                                       gaagGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTACTGGCCAt                                         >  2:3795823/1‑148 (MQ=255)
                                                                                                       gaagGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCAt                                         >  2:2814905/1‑148 (MQ=255)
                                                                                                       gaagGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCGTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCAt                                         >  2:3701105/1‑148 (MQ=255)
                                                                                                        aagGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATg                                        <  2:2708976/148‑1 (MQ=255)
                                                                                                         agGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATgg                                       >  2:564110/1‑148 (MQ=255)
                                                                                                          ggTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCAAGCAGGATAGCTAACACGTGTACGCTGAAAAAGTCAGATCAGTTCGGAATAAACCATTTCAGCCAACTTAATGTCCATGGa                                      >  2:2863728/1‑148 (MQ=255)
                                                                                                           gTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGaa                                     <  1:2331925/148‑1 (MQ=255)
                                                                                                            tcttctTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATATCTAACACGTGTACGCTGAACAAGTACGATCAGTTCGGAATAAAACAGTTAAGCCAACGTAATGGCCATGGaaa                                    >  1:2247383/1‑148 (MQ=255)
                                                                                                            tcttctTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGaaa                                    <  2:3381920/148‑1 (MQ=255)
                                                                                                            tcttctTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGaaa                                    <  2:3295596/148‑1 (MQ=255)
                                                                                                            tcttctTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGaaa                                    <  1:2578356/148‑1 (MQ=255)
                                                                                                             cttcttTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGaaaa                                   <  1:1874939/148‑1 (MQ=255)
                                                                                                                  tttGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACGAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTg                              >  1:2655585/1‑148 (MQ=255)
                                                                                                                  tttGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTg                              <  1:1894017/148‑1 (MQ=255)
                                                                                                                   ttGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGa                             <  1:1401436/148‑1 (MQ=255)
                                                                                                                     gTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACg                           <  2:2977843/148‑1 (MQ=255)
                                                                                                                     gTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACg                           <  2:332916/148‑1 (MQ=255)
                                                                                                                     gTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACg                           >  1:3364544/1‑148 (MQ=255)
                                                                                                                         aGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGtcct                       >  2:2744135/1‑148 (MQ=255)
                                                                                                                          ggATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGtcctt                      <  2:3513626/148‑1 (MQ=255)
                                                                                                                          ggATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGtcctt                      <  2:1279839/148‑1 (MQ=255)
                                                                                                                           gATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGtccttc                     <  1:2356197/148‑1 (MQ=255)
                                                                                                                            aTTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGtccttcc                    >  2:1089737/1‑148 (MQ=255)
                                                                                                                            aTTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGtccttcc                    <  2:1304238/148‑1 (MQ=255)
                                                                                                                                 cgTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAAGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTg               >  1:512413/1‑148 (MQ=255)
                                                                                                                                   tttATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCg             >  1:3149713/1‑148 (MQ=255)
                                                                                                                                     tATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGAt           <  1:292524/148‑1 (MQ=255)
                                                                                                                                        cTACCATGTTCTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCt        >  1:1148982/1‑148 (MQ=255)
                                                                                                                                        cTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCt        >  1:71878/1‑148 (MQ=255)
                                                                                                                                           ccATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTgc     <  2:2922626/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCACCCGTTTCGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTTCTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCG  >  CP009273/1011784‑1012073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTACCACCCGTTTCGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTTCTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATAC  >  CP009273/1011781‑1012077
                                                                                                                                                    |                                                                                                                                                    
TTACCACCCGTTTCGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTAC                                                                                                                                                     >  SRR19973069.889640/1‑150 (MQ=60)
    CACCCGTTTCGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTG                                                                                                                                                 >  SRR19973069.3311363/1‑150 (MQ=60)
    CACCCGTTTCGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTG                                                                                                                                                 >  SRR19973069.2045134/1‑150 (MQ=60)
     ACCCGTTTCGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGT                                                                                                                                                <  SRR19973069.3146289/150‑1 (MQ=60)
     ACCCGTTTCGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGT                                                                                                                                                <  SRR19973069.3146278/150‑1 (MQ=60)
         GTTTCGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGC                                                                                                                                            <  SRR19973069.1955001/150‑1 (MQ=60)
            TCGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTT                                                                                                                                         >  SRR19973069.3750521/1‑150 (MQ=60)
             CGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTA                                                                                                                                        >  SRR19973069.283969/1‑150 (MQ=60)
             CGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTA                                                                                                                                        >  SRR19973069.283981/1‑150 (MQ=60)
             CGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTA                                                                                                                                        >  SRR19973069.2225519/1‑150 (MQ=60)
              GGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTAT                                                                                                                                       <  SRR19973069.165118/150‑1 (MQ=60)
               GTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATT                                                                                                                                      <  SRR19973069.378415/150‑1 (MQ=60)
                TCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTT                                                                                                                                     <  SRR19973069.61172/150‑1 (MQ=60)
                TCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTT                                                                                                                                     <  SRR19973069.2586761/150‑1 (MQ=60)
                 CATTTTGACCACACGGTCCATCATCATCATGTTCGGTGCTGTCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTATTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTT                                                                                                                                    <  SRR19973069.2315497/150‑1 (MQ=60)
                  ATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTA                                                                                                                                   >  SRR19973069.2643791/1‑150 (MQ=60)
                    TTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATT                                                                                                                                 <  SRR19973069.2789848/150‑1 (MQ=60)
                    TTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATT                                                                                                                                 <  SRR19973069.981322/150‑1 (MQ=60)
                      TGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGA                                                                                                                               >  SRR19973069.1188426/1‑150 (MQ=60)
                          CACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCA                                                                                                                           <  SRR19973069.455568/150‑1 (MQ=60)
                           ACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTGCCATGTACTCTGTAAGCCTTATTTTATTGAAGCAC                                                                                                                          <  SRR19973069.3319234/150‑1 (MQ=60)
                           ACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCAC                                                                                                                          <  SRR19973069.2930005/150‑1 (MQ=60)
                           ACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCAC                                                                                                                          >  SRR19973069.664676/1‑150 (MQ=60)
                           ACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCAC                                                                                                                          <  SRR19973069.2929674/150‑1 (MQ=60)
                            CACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACG                                                                                                                         <  SRR19973069.1456543/150‑1 (MQ=60)
                            CACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACG                                                                                                                         <  SRR19973069.1456055/150‑1 (MQ=60)
                            CACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACG                                                                                                                         <  SRR19973069.2131820/150‑1 (MQ=60)
                                  CCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGAT                                                                                                                   <  SRR19973069.1748867/150‑1 (MQ=60)
                                  CCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGAT                                                                                                                   >  SRR19973069.2561876/1‑150 (MQ=60)
                                  CCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGAT                                                                                                                   >  SRR19973069.2206720/1‑150 (MQ=60)
                                  CCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGAT                                                                                                                   >  SRR19973069.2206702/1‑150 (MQ=60)
                                   CATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGAGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATA                                                                                                                  <  SRR19973069.3291866/150‑1 (MQ=60)
                                   CATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGAGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATA                                                                                                                  <  SRR19973069.3291861/150‑1 (MQ=60)
                                     TCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGC                                                                                                                <  SRR19973069.3107657/150‑1 (MQ=60)
                                     TCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGC                                                                                                                <  SRR19973069.3106000/150‑1 (MQ=60)
                                      CATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCT                                                                                                               <  SRR19973069.3157894/150‑1 (MQ=60)
                                      CATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCT                                                                                                               <  SRR19973069.1241092/150‑1 (MQ=60)
                                      CATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCT                                                                                                               >  SRR19973069.638545/1‑150 (MQ=60)
                                       ATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTA                                                                                                              >  SRR19973069.104509/1‑150 (MQ=60)
                                       ATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTA                                                                                                              >  SRR19973069.3536281/1‑150 (MQ=60)
                                          AGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACA                                                                                                           >  SRR19973069.3665329/1‑150 (MQ=60)
                                          AGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACA                                                                                                           >  SRR19973069.3665243/1‑150 (MQ=60)
                                             ATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGT                                                                                                        >  SRR19973069.281153/1‑150 (MQ=60)
                                             ATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGT                                                                                                        >  SRR19973069.2020428/1‑150 (MQ=60)
                                               GTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGT                                                                                                      >  SRR19973069.3018501/1‑150 (MQ=60)
                                                 TCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTAC                                                                                                    <  SRR19973069.3844356/150‑1 (MQ=60)
                                                  CGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTTCTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACG                                                                                                   >  SRR19973069.155483/1‑150 (MQ=60)
                                                  CGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACG                                                                                                   <  SRR19973069.916675/150‑1 (MQ=60)
                                                    GTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCT                                                                                                 >  SRR19973069.3384215/1‑150 (MQ=60)
                                                       CTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAA                                                                                              <  SRR19973069.1809036/150‑1 (MQ=60)
                                                           CAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAG                                                                                          >  SRR19973069.2958123/1‑150 (MQ=60)
                                                           CAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAG                                                                                          >  SRR19973069.2958095/1‑150 (MQ=60)
                                                            AATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGT                                                                                         <  SRR19973069.3774455/150‑1 (MQ=60)
                                                             ATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTC                                                                                        <  SRR19973069.949661/150‑1 (MQ=60)
                                                                  GGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATC                                                                                   >  SRR19973069.2963226/1‑150 (MQ=60)
                                                                   GGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCA                                                                                  <  SRR19973069.3670132/150‑1 (MQ=60)
                                                                   GGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCA                                                                                  >  SRR19973069.794761/1‑150 (MQ=60)
                                                                      CTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTT                                                                               >  SRR19973069.1098709/1‑150 (MQ=60)
                                                                      CTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTT                                                                               >  SRR19973069.1098755/1‑150 (MQ=60)
                                                                       TTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTC                                                                              <  SRR19973069.3329095/150‑1 (MQ=60)
                                                                       TTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTC                                                                              <  SRR19973069.2365863/150‑1 (MQ=60)
                                                                       TTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTC                                                                              <  SRR19973069.2365870/150‑1 (MQ=60)
                                                                         TAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGG                                                                            >  SRR19973069.1640723/1‑150 (MQ=60)
                                                                         TAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGG                                                                            >  SRR19973069.1641345/1‑150 (MQ=60)
                                                                          AGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGA                                                                           >  SRR19973069.1468413/1‑150 (MQ=60)
                                                                          AGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGA                                                                           >  SRR19973069.1839927/1‑150 (MQ=60)
                                                                            CGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAAT                                                                         <  SRR19973069.1466388/150‑1 (MQ=60)
                                                                               CAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAA                                                                      >  SRR19973069.56785/1‑150 (MQ=60)
                                                                                 AACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACC                                                                    <  SRR19973069.2376906/150‑1 (MQ=60)
                                                                                      TTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTC                                                               <  SRR19973069.999241/150‑1 (MQ=60)
                                                                                      TTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTC                                                               <  SRR19973069.3072411/150‑1 (MQ=60)
                                                                                              GACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACG                                                       >  SRR19973069.2173082/1‑150 (MQ=60)
                                                                                              GACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGGTCGGAATAAACCAGTTCAGCCAACG                                                       >  SRR19973069.2173299/1‑150 (MQ=60)
                                                                                                    AGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGG                                                 <  SRR19973069.2966814/150‑1 (MQ=60)
                                                                                                    AGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGG                                                 >  SRR19973069.845948/1‑150 (MQ=60)
                                                                                                       CAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCA                                              <  SRR19973069.1859102/150‑1 (MQ=60)
                                                                                                       CAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCA                                              <  SRR19973069.1149346/150‑1 (MQ=60)
                                                                                                         AGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATG                                            <  SRR19973069.2629608/150‑1 (MQ=60)
                                                                                                         AGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATG                                            <  SRR19973069.2629653/150‑1 (MQ=60)
                                                                                                           AAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGA                                          >  SRR19973069.532116/1‑150 (MQ=60)
                                                                                                            AGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAA                                         >  SRR19973069.2371661/1‑150 (MQ=60)
                                                                                                            AGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAA                                         >  SRR19973069.2373353/1‑150 (MQ=60)
                                                                                                             GGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATATCTAACACGTGTACGCTGAACAAGTACGATCAGTTCGGAATAAAACAGTTAAGCCAACGTAATGGCCATGGAAA                                        >  SRR19973069.2262523/1‑150 (MQ=60)
                                                                                                              GTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAA                                       <  SRR19973069.2347799/150‑1 (MQ=60)
                                                                                                               TCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAAC                                      <  SRR19973069.2595481/150‑1 (MQ=60)
                                                                                                                CTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACG                                     >  SRR19973069.3295967/1‑150 (MQ=60)
                                                                                                                CTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACG                                     <  SRR19973069.1888088/150‑1 (MQ=60)
                                                                                                                CTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACG                                     <  SRR19973069.1887981/150‑1 (MQ=60)
                                                                                                                   CTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACGAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTG                                  >  SRR19973069.2673381/1‑150 (MQ=60)
                                                                                                                   CTTTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTG                                  >  SRR19973069.2673098/1‑150 (MQ=60)
                                                                                                                     TTTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGAC                                <  SRR19973069.1907137/150‑1 (MQ=60)
                                                                                                                      TTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACG                               >  SRR19973069.3386554/1‑150 (MQ=60)
                                                                                                                      TTGTATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACG                               <  SRR19973069.1411210/150‑1 (MQ=60)
                                                                                                                         TATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCC                            >  SRR19973069.3078115/1‑150 (MQ=60)
                                                                                                                          ATAGGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATTAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCT                           <  SRR19973069.1837257/150‑1 (MQ=60)
                                                                                                                             GGATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCC                        >  SRR19973069.1270912/1‑150 (MQ=60)
                                                                                                                              GATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCT                       <  SRR19973069.2372211/150‑1 (MQ=60)
                                                                                                                              GATTCGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCT                       <  SRR19973069.1461060/150‑1 (MQ=60)
                                                                                                                                  CGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTG                   <  SRR19973069.3477962/150‑1 (MQ=60)
                                                                                                                                  CGCGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAAGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTG                   >  SRR19973069.516062/1‑150 (MQ=60)
                                                                                                                                    CGTTTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCG                 >  SRR19973069.3170299/1‑150 (MQ=60)
                                                                                                                                       TTATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATG              >  SRR19973069.2176058/1‑150 (MQ=60)
                                                                                                                                        TATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGC             <  SRR19973069.294534/150‑1 (MQ=60)
                                                                                                                                         ATCTACCATGTTCTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCT            >  SRR19973069.1156997/1‑150 (MQ=60)
                                                                                                                                         ATCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCT            >  SRR19973069.72691/1‑150 (MQ=60)
                                                                                                                                          TCTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACTCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTT           <  SRR19973069.2518210/150‑1 (MQ=60)
                                                                                                                                           CTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTG          <  SRR19973069.780182/150‑1 (MQ=60)
                                                                                                                                           CTACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTG          >  SRR19973069.435175/1‑150 (MQ=60)
                                                                                                                                             ACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCG        <  SRR19973069.455613/150‑1 (MQ=60)
                                                                                                                                             ACCATGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCG        <  SRR19973069.966428/150‑1 (MQ=60)
                                                                                                                                                 TGTACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGAT    <  SRR19973069.275395/150‑1 (MQ=60)
                                                                                                                                                   TACTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATAC  <  SRR19973069.2439042/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
TTACCACCCGTTTCGGTCATTTTGACCACACGGTCCATCATCAGCATGTTCGGTGCTGGCAATTGCGGGCCTTTAGCGCCAAACAGTTCACCGCGACCAGAGGCAAGAAGGTCTTCTTTTGTATAGGATTCGCGTTTATCTACCATGTTCTCTGTAAGCCTTATTTTATTGAAGCACGCAGGATAGCTAACACGTGTACGCTGAACAAGTCCGATCAGTTCGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATAC  >  CP009273/1011781‑1012077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑