Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,146,692 A→T 100% T189T (ACA→ACT)  fabG → 3‑oxoacyl‑[acyl‑carrier‑protein] reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,146,6920AT97.9% 773.3 / 4.3 235T189T (ACA→ACT) fabG3‑oxoacyl‑[acyl‑carrier‑protein] reductase
Reads supporting (aligned to +/- strand):  ref base A (2/3);  new base T (118/112);  total (120/115)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 235 total aligned reads displayed.

GTACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGT  >  CP009273/1146547‑1146835
                                                                                                                                                 |                                                                                                                                               
 tACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGt                                                                                                                                              >  2:1828180/1‑148 (MQ=255)
     aTGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTgcgc                                                                                                                                          >  1:1361108/1‑148 (MQ=255)
      tGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCt                                                                                                                                         >  2:2326180/1‑148 (MQ=255)
       gggAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTg                                                                                                                                        <  2:3371116/148‑1 (MQ=255)
       gggAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGCCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTg                                                                                                                                        <  1:761869/148‑1 (MQ=255)
         gAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAg                                                                                                                                      >  1:2306003/1‑148 (MQ=255)
           aaTGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCg                                                                                                                                    <  2:3231163/148‑1 (MQ=255)
            aTGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGa                                                                                                                                   >  1:380840/1‑148 (MQ=255)
            aTGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGa                                                                                                                                   <  2:1895928/148‑1 (MQ=255)
            aTGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGa                                                                                                                                   <  2:1937234/148‑1 (MQ=255)
                cGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGAc                                                                                                                               <  1:3627728/148‑1 (MQ=255)
                 ggTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGAcc                                                                                                                              <  2:2925406/148‑1 (MQ=255)
                 ggTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGAcc                                                                                                                              <  1:2107383/148‑1 (MQ=255)
                  gTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCa                                                                                                                             >  1:1909350/1‑148 (MQ=255)
                       gCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTg                                                                                                                        >  1:3200364/1‑148 (MQ=255)
                            cTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGt                                                                                                                   >  1:3312592/1‑148 (MQ=255)
                                gCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATcc                                                                                                               <  1:3076256/148‑1 (MQ=255)
                                   gcggcgAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTgg                                                                                                            <  2:4126/148‑1 (MQ=255)
                                   gcggcgAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTgg                                                                                                            <  1:2477170/148‑1 (MQ=255)
                                       cgAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCa                                                                                                        >  2:2425620/1‑148 (MQ=255)
                                          aaGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGt                                                                                                     >  1:3650988/1‑148 (MQ=255)
                                           aGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGtt                                                                                                    >  1:564239/1‑148 (MQ=255)
                                           aGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGtt                                                                                                    >  1:2905852/1‑148 (MQ=255)
                                            gCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTc                                                                                                   <  2:1609187/148‑1 (MQ=255)
                                            gCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTc                                                                                                   >  1:1990950/1‑148 (MQ=255)
                                            gCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTc                                                                                                   <  1:3515985/148‑1 (MQ=255)
                                            gCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTc                                                                                                   >  2:907787/1‑148 (MQ=255)
                                             cGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTcc                                                                                                  <  2:371300/148‑1 (MQ=255)
                                                gCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGc                                                                                               >  2:1068249/1‑148 (MQ=255)
                                                 cTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCg                                                                                              <  2:3112017/148‑1 (MQ=255)
                                                     aTCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTc                                                                                          >  2:2552587/1‑148 (MQ=255)
                                                     aTCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTc                                                                                          >  1:2995234/1‑148 (MQ=255)
                                                      tCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCg                                                                                         >  2:618804/1‑148 (MQ=255)
                                                         gCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCt                                                                                      <  1:113056/148‑1 (MQ=255)
                                                          cTTCAGTAAATCACTGGCGCACGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTc                                                                                     >  1:3372385/1‑148 (MQ=255)
                                                           ttCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTcg                                                                                    >  2:1013236/1‑148 (MQ=255)
                                                                    tCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGcac                                                                           >  1:3093844/1‑148 (MQ=255)
                                                                      aCTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAg                                                                         >  2:2988411/1‑148 (MQ=255)
                                                                       cTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAgg                                                                        <  2:1579331/148‑1 (MQ=255)
                                                                       cTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAgg                                                                        >  1:2242872/1‑148 (MQ=255)
                                                                             cgcgAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCg                                                                  <  2:2922238/148‑1 (MQ=255)
                                                                             cgcgAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCg                                                                  <  2:2921273/148‑1 (MQ=255)
                                                                             cgcgAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCg                                                                  <  1:3474030/148‑1 (MQ=255)
                                                                               cgAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTCTCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAACTCGcc                                                                >  2:3573706/1‑148 (MQ=255)
                                                                                 aaGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCaa                                                              <  1:514985/148‑1 (MQ=255)
                                                                                  aGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAAc                                                             >  2:3274562/1‑148 (MQ=255)
                                                                                    ttGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAAcgc                                                           >  1:172810/1‑148 (MQ=255)
                                                                                    ttGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAAcgc                                                           >  1:1748669/1‑148 (MQ=255)
                                                                                     tGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGAGCACAGGAAATCGCCAAcgcg                                                          >  2:2526119/1‑148 (MQ=255)
                                                                                            cgcgGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGGTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCAt                                                   <  1:1433686/148‑1 (MQ=255)
                                                                                              cgGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTc                                                 <  2:3327613/148‑1 (MQ=255)
                                                                                              cgGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTc                                                 <  1:837493/148‑1 (MQ=255)
                                                                                               ggTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTcc                                                <  1:1613778/148‑1 (MQ=255)
                                                                                                gTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCt                                               <  2:2539442/148‑1 (MQ=255)
                                                                                                gTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCt                                               <  2:1443077/148‑1 (MQ=255)
                                                                                                 tATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTg                                              >  1:2358010/1‑148 (MQ=255)
                                                                                                  aTTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTgg                                             >  1:2161589/1‑148 (MQ=255)
                                                                                                     aCTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCAt                                          >  2:652712/1‑148 (MQ=255)
                                                                                                     aCTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCAt                                          >  2:2232315/1‑148 (MQ=255)
                                                                                                     aCTGTAAACGTTGGTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCAt                                          <  1:150830/148‑1 (MQ=255)
                                                                                                       tGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATcc                                        <  1:3783514/148‑1 (MQ=255)
                                                                                                       tGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATcc                                        <  1:1043921/148‑1 (MQ=255)
                                                                                                         tAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCTCTGAGCGTTGACCAGCGTGCGGGTATCCTGGATCAGGTTCCTGCGGGTCGCATCGGCGGCGGACAGGAAATCGCCTACGCGGTTGCATTCCTGGCATCcga                                      <  2:1193084/148‑1 (MQ=255)
                                                                                                           aaCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcgacg                                    <  1:981217/148‑1 (MQ=255)
                                                                                                            aCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcgacga                                   <  2:960134/148‑1 (MQ=255)
                                                                                                            aCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcgacga                                   <  2:3200364/148‑1 (MQ=255)
                                                                                                            aCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcgacga                                   >  1:1629571/1‑148 (MQ=255)
                                                                                                            aCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcgacga                                   >  1:2348842/1‑148 (MQ=255)
                                                                                                             cGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGaa                                  >  1:1173791/1‑148 (MQ=255)
                                                                                                              gttgttGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAag                                 <  2:2635324/148‑1 (MQ=255)
                                                                                                                tgttgCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAagca                               >  1:432102/1‑148 (MQ=255)
                                                                                                                 ggtgCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGGGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAagcag                              <  2:502778/146‑1 (MQ=255)
                                                                                                                  ttgCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAagcagc                             >  1:3350124/1‑148 (MQ=255)
                                                                                                                   tgCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCt                            >  2:1988858/1‑148 (MQ=255)
                                                                                                                    gCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCtt                           <  1:1287031/148‑1 (MQ=255)
                                                                                                                      tCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTAc                         <  1:6226/148‑1 (MQ=255)
                                                                                                                        cGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACAt                       <  1:2552587/148‑1 (MQ=255)
                                                                                                                         gggCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATc                      >  1:1842365/1‑148 (MQ=255)
                                                                                                                         gggCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATc                      >  1:703506/1‑148 (MQ=255)
                                                                                                                         gggCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATc                      >  1:3733615/1‑148 (MQ=255)
                                                                                                                          ggCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCa                     >  2:2236346/1‑148 (MQ=255)
                                                                                                                           gCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCAc                    >  2:3488111/1‑148 (MQ=255)
                                                                                                                            cTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGTGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACg                   >  2:3075200/1‑148 (MQ=255)
                                                                                                                            cTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGGTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACg                   <  1:2819670/148‑1 (MQ=255)
                                                                                                                                  tGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGaa             >  1:1700506/1‑148 (MQ=255)
                                                                                                                                  tGAAACGGACATGAATCGTGCGATGAGCGATGACAATCGTGCGGGTATCATGGCGCAGGTTACTGCGGGTCGCATCCGCGGCGCACAGTAAATCTCCAAAGCGTCTGCATTCATGGCATCCGACGAAGCAGCTTACATCACGGGTGaa             >  2:2027323/1‑148 (MQ=255)
                                                                                                                                     aaCGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACt          <  2:380840/148‑1 (MQ=255)
                                                                                                                                      aCGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACtt         <  1:2344940/148‑1 (MQ=255)
                                                                                                                                      aCGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACtt         <  2:3160836/148‑1 (MQ=255)
                                                                                                                                      aCGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACtt         <  2:2049642/148‑1 (MQ=255)
                                                                                                                                      aCGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACtt         <  2:1842365/148‑1 (MQ=255)
                                                                                                                                       cGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACttt        >  1:383280/1‑148 (MQ=255)
                                                                                                                                       cGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACttt        <  2:736859/148‑1 (MQ=255)
                                                                                                                                        ggACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTg       <  2:1361108/148‑1 (MQ=255)
                                                                                                                                        ggACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTg       <  2:3352479/148‑1 (MQ=255)
                                                                                                                                         gACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGc      <  1:2425620/148‑1 (MQ=255)
                                                                                                                                           cATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCAt    >  2:3006219/1‑148 (MQ=255)
                                                                                                                                            aTGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCAtg   >  1:2735291/1‑148 (MQ=255)
                                                                                                                                            aTGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCAtg   <  1:635809/148‑1 (MQ=255)
                                                                                                                                             tGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCAtgt  >  1:2190726/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GTACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGT  >  CP009273/1146547‑1146835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTGGTACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACG  >  CP009273/1146543‑1146840
                                                                                                                                                     |                                                                                                                                                    
GTTGGTACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACT                                                                                                                                                      <  SRR19973069.339573/150‑1 (MQ=60)
GTTGGTACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACT                                                                                                                                                      <  SRR19973069.339552/150‑1 (MQ=60)
  TGGTACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCG                                                                                                                                                    >  SRR19973069.544669/1‑150 (MQ=60)
  TGGTACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCG                                                                                                                                                    >  SRR19973069.64399/1‑150 (MQ=60)
  TGGTACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCG                                                                                                                                                    >  SRR19973069.975613/1‑150 (MQ=60)
       CCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGC                                                                                                                                               >  SRR19973069.3374418/1‑150 (MQ=60)
       CCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGC                                                                                                                                               >  SRR19973069.1370565/1‑150 (MQ=60)
           GGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAG                                                                                                                                           <  SRR19973069.1692232/150‑1 (MQ=60)
           GGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAG                                                                                                                                           <  SRR19973069.1692427/150‑1 (MQ=60)
           GGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAG                                                                                                                                           >  SRR19973069.2321691/1‑150 (MQ=60)
           GGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGCCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAG                                                                                                                                           <  SRR19973069.767250/150‑1 (MQ=60)
             GAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCG                                                                                                                                         >  SRR19973069.3428880/1‑150 (MQ=60)
              AAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGA                                                                                                                                        >  SRR19973069.2323242/1‑150 (MQ=60)
              AAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGA                                                                                                                                        >  SRR19973069.383677/1‑150 (MQ=60)
              AAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGA                                                                                                                                        >  SRR19973069.383619/1‑150 (MQ=60)
                 TGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGA                                                                                                                                     >  SRR19973069.1955707/1‑150 (MQ=60)
                 TGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGA                                                                                                                                     <  SRR19973069.1131333/150‑1 (MQ=60)
                    CGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCA                                                                                                                                  <  SRR19973069.3651443/150‑1 (MQ=60)
                    CGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCA                                                                                                                                  <  SRR19973069.1745826/150‑1 (MQ=60)
                    CGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCA                                                                                                                                  >  SRR19973069.1922528/1‑150 (MQ=60)
                     GGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAG                                                                                                                                 <  SRR19973069.2121919/150‑1 (MQ=60)
                     GGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAG                                                                                                                                 >  SRR19973069.56565/1‑150 (MQ=60)
                      GTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGC                                                                                                                                >  SRR19973069.742076/1‑150 (MQ=60)
                         AGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTG                                                                                                                             >  SRR19973069.3221174/1‑150 (MQ=60)
                         AGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTG                                                                                                                             >  SRR19973069.1590051/1‑150 (MQ=60)
                              AACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGT                                                                                                                        >  SRR19973069.3334302/1‑150 (MQ=60)
                                CTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTAT                                                                                                                      >  SRR19973069.3241073/1‑150 (MQ=60)
                                    GCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTG                                                                                                                  <  SRR19973069.3096565/150‑1 (MQ=60)
                                       GCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCG                                                                                                               <  SRR19973069.2493766/150‑1 (MQ=60)
                                            GAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGT                                                                                                          >  SRR19973069.3674900/1‑150 (MQ=60)
                                             AAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTT                                                                                                         >  SRR19973069.2925500/1‑150 (MQ=60)
                                             AAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTT                                                                                                         >  SRR19973069.2925063/1‑150 (MQ=60)
                                             AAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTT                                                                                                         >  SRR19973069.799377/1‑150 (MQ=60)
                                             AAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTT                                                                                                         >  SRR19973069.568168/1‑150 (MQ=60)
                                              AAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTC                                                                                                        >  SRR19973069.2004830/1‑150 (MQ=60)
                                               AGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCC                                                                                                       >  SRR19973069.2459586/1‑150 (MQ=60)
                                                GCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAATTTGCGTCACGCGTTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCT                                                                                                      <  SRR19973069.665551/150‑1 (MQ=60)
                                                GCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCT                                                                                                      <  SRR19973069.3538669/150‑1 (MQ=60)
                                                GCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCT                                                                                                      <  SRR19973069.1318331/150‑1 (MQ=60)
                                                GCGGGATTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCT                                                                                                      <  SRR19973069.3538661/150‑1 (MQ=60)
                                                  GGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGATACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGC                                                                                                    <  SRR19973069.620730/150‑1 (MQ=60)
                                                     CTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGG                                                                                                 >  SRR19973069.3390164/1‑150 (MQ=60)
                                                       TGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTC                                                                                               >  SRR19973069.3015197/1‑150 (MQ=60)
                                                       TGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTC                                                                                               >  SRR19973069.3015117/1‑150 (MQ=60)
                                                           CGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCT                                                                                           <  SRR19973069.31818/150‑1 (MQ=60)
                                                            GGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTC                                                                                          <  SRR19973069.2182023/150‑1 (MQ=60)
                                                            GGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTC                                                                                          <  SRR19973069.1903284/150‑1 (MQ=60)
                                                            GGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTC                                                                                          <  SRR19973069.3839570/150‑1 (MQ=60)
                                                            GGCTTCAGTAAATCACTGGCGCACGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTC                                                                                          >  SRR19973069.1478392/1‑150 (MQ=60)
                                                            GGCTTCAGTAAATCACTGGCGCACGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTC                                                                                          >  SRR19973069.3394436/1‑150 (MQ=60)
                                                             GCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCG                                                                                         <  SRR19973069.114161/150‑1 (MQ=60)
                                                                 CAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGG                                                                                     >  SRR19973069.410564/1‑150 (MQ=60)
                                                                      AATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCAC                                                                                >  SRR19973069.3118929/1‑150 (MQ=60)
                                                                      AATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCAC                                                                                >  SRR19973069.3114234/1‑150 (MQ=60)
                                                                        TCACTGGCGCGCGAAGTTGCGTCACGCGGTAGTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAG                                                                              <  SRR19973069.2521624/150‑1 (MQ=60)
                                                                         CACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGG                                                                             >  SRR19973069.2257981/1‑150 (MQ=60)
                                                                         CACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGG                                                                             >  SRR19973069.2257471/1‑150 (MQ=60)
                                                                             GGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAAT                                                                         <  SRR19973069.1165063/150‑1 (MQ=60)
                                                                                 CGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCC                                                                     <  SRR19973069.3496487/150‑1 (MQ=60)
                                                                                    GAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAAC                                                                  <  SRR19973069.1795392/150‑1 (MQ=60)
                                                                                     AAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACG                                                                 <  SRR19973069.518653/150‑1 (MQ=60)
                                                                                     AAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACG                                                                 >  SRR19973069.3673534/1‑150 (MQ=60)
                                                                                      AGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGC                                                                >  SRR19973069.174275/1‑150 (MQ=60)
                                                                                      AGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGC                                                                >  SRR19973069.1760976/1‑150 (MQ=60)
                                                                                           CGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTG                                                           <  SRR19973069.3000330/150‑1 (MQ=60)
                                                                                             TCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCA                                                         <  SRR19973069.2396135/150‑1 (MQ=60)
                                                                                                CGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGGTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTC                                                      <  SRR19973069.1443692/150‑1 (MQ=60)
                                                                                                 GCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCC                                                     <  SRR19973069.866453/150‑1 (MQ=60)
                                                                                                  CGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCT                                                    <  SRR19973069.843378/150‑1 (MQ=60)
                                                                                                   GGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTG                                                   <  SRR19973069.1624643/150‑1 (MQ=60)
                                                                                                   GGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTG                                                   >  SRR19973069.2374032/1‑150 (MQ=60)
                                                                                                    GTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGG                                                  >  SRR19973069.631846/1‑150 (MQ=60)
                                                                                                    GTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGG                                                  >  SRR19973069.2176378/1‑150 (MQ=60)
                                                                                                    GTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGG                                                  >  SRR19973069.2217583/1‑150 (MQ=60)
                                                                                                         ACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCC                                             <  SRR19973069.3814472/150‑1 (MQ=60)
                                                                                                         ACTGTAAACGTTGGTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCC                                             <  SRR19973069.152159/150‑1 (MQ=60)
                                                                                                          CTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCG                                            >  SRR19973069.1234873/1‑150 (MQ=60)
                                                                                                          CTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCG                                            >  SRR19973069.1234824/1‑150 (MQ=60)
                                                                                                          CTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCG                                            >  SRR19973069.3759843/1‑150 (MQ=60)
                                                                                                           TGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGA                                           <  SRR19973069.1051176/150‑1 (MQ=60)
                                                                                                           TGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGA                                           <  SRR19973069.3808067/150‑1 (MQ=60)
                                                                                                              AAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGA                                        >  SRR19973069.1640685/1‑150 (MQ=60)
                                                                                                              AAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGA                                        >  SRR19973069.2364612/1‑150 (MQ=60)
                                                                                                              AAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGA                                        >  SRR19973069.3100776/1‑150 (MQ=60)
                                                                                                              AAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGA                                        >  SRR19973069.2364819/1‑150 (MQ=60)
                                                                                                              AAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGA                                        >  SRR19973069.1638189/1‑150 (MQ=60)
                                                                                                              AAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCAGCCGACGA                                        >  SRR19973069.2364823/1‑150 (MQ=60)
                                                                                                               AACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAA                                       <  SRR19973069.987841/150‑1 (MQ=60)
                                                                                                               AACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAA                                       <  SRR19973069.988111/150‑1 (MQ=60)
                                                                                                               AACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAA                                       >  SRR19973069.1181923/1‑150 (MQ=60)
                                                                                                                ACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAG                                      <  SRR19973069.1725527/150‑1 (MQ=60)
                                                                                                                ACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAG                                      <  SRR19973069.2898406/150‑1 (MQ=60)
                                                                                                                 CGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTNCGCTGAGCGATGACCAGCGTGCGGNTANCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCNCCAACGCGGTTGCATTCCTGGCATCCGACGAAGC                                     >  SRR19973069.2425121/1‑150 (MQ=60)
                                                                                                                  GTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCA                                    >  SRR19973069.436145/1‑150 (MQ=60)
                                                                                                                  GTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCA                                    >  SRR19973069.435277/1‑150 (MQ=60)
                                                                                                                  GTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCA                                    <  SRR19973069.141674/150‑1 (MQ=60)
                                                                                                                   TTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAG                                   <  SRR19973069.3731256/150‑1 (MQ=60)
                                                                                                                   TTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAG                                   <  SRR19973069.3731280/150‑1 (MQ=60)
                                                                                                                    TGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGC                                  >  SRR19973069.3371768/1‑150 (MQ=60)
                                                                                                                    TGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGC                                  >  SRR19973069.3551803/1‑150 (MQ=60)
                                                                                                                    TGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGC                                  >  SRR19973069.3372051/1‑150 (MQ=60)
                                                                                                                      TTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTT                                <  SRR19973069.2262055/150‑1 (MQ=60)
                                                                                                                        GCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTAC                              <  SRR19973069.1295636/150‑1 (MQ=60)
                                                                                                                          TCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACAT                            <  SRR19973069.2736520/150‑1 (MQ=60)
                                                                                                                          TCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACAT                            <  SRR19973069.179739/150‑1 (MQ=60)
                                                                                                                          TCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACAT                            <  SRR19973069.6333/150‑1 (MQ=60)
                                                                                                                           CCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATC                           >  SRR19973069.1855245/1‑150 (MQ=60)
                                                                                                                           CCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATC                           >  SRR19973069.708454/1‑150 (MQ=60)
                                                                                                                           CCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATC                           >  SRR19973069.2063871/1‑150 (MQ=60)
                                                                                                                           CCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATC                           >  SRR19973069.3757954/1‑150 (MQ=60)
                                                                                                                            CGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCA                          <  SRR19973069.2569443/150‑1 (MQ=60)
                                                                                                                                CTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGGTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGG                      <  SRR19973069.2838330/150‑1 (MQ=60)
                                                                                                                                    ATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAA                  >  SRR19973069.1712403/1‑150 (MQ=60)
                                                                                                                                      TGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAAC                <  SRR19973069.354960/150‑1 (MQ=60)
                                                                                                                                       GAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACT               >  SRR19973069.2150881/1‑150 (MQ=60)
                                                                                                                                       GAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACT               >  SRR19973069.209386/1‑150 (MQ=60)
                                                                                                                                         AACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTT             >  SRR19973069.386083/1‑150 (MQ=60)
                                                                                                                                          ACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTG            <  SRR19973069.2360885/150‑1 (MQ=60)
                                                                                                                                            GGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCA          >  SRR19973069.1503689/1‑150 (MQ=60)
                                                                                                                                             GACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCAT         <  SRR19973069.2441991/150‑1 (MQ=60)
                                                                                                                                              ACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATG        <  SRR19973069.2332298/150‑1 (MQ=60)
                                                                                                                                              ACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATG        >  SRR19973069.2753410/1‑150 (MQ=60)
                                                                                                                                              ACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATG        >  SRR19973069.2753593/1‑150 (MQ=60)
                                                                                                                                              ACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATG        >  SRR19973069.2753675/1‑150 (MQ=60)
                                                                                                                                               CATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGT       >  SRR19973069.2205633/1‑150 (MQ=60)
                                                                                                                                                ATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTG      <  SRR19973069.639998/150‑1 (MQ=60)
                                                                                                                                                 TGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGA     >  SRR19973069.845472/1‑150 (MQ=60)
                                                                                                                                                    CTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACG  >  SRR19973069.2502365/1‑150 (MQ=60)
                                                                                                                                                    CTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACG  <  SRR19973069.2877544/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GTTGGTACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACG  >  CP009273/1146543‑1146840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑