Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,214,563 A→T 100% T270S (ACC→TCC)  metH → homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,214,5630AT100.0% 494.3 / ‑6.7 149T270S (ACC→TCC) metHhomocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (68/80);  total (68/81)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 149 total aligned reads displayed.

CAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATA  >  CP009273/4214431‑4214707
                                                                                                                                    |                                                                                                                                                
cAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACg                                                                                                                                   >  2:919436/1‑148 (MQ=255)
cAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACg                                                                                                                                   >  2:2259288/1‑148 (MQ=255)
cAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACg                                                                                                                                   >  2:2832997/1‑148 (MQ=255)
  gACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGcc                                                                                                                                 >  2:1649774/1‑148 (MQ=255)
     caccGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCggg                                                                                                                              >  2:806337/1‑148 (MQ=255)
     caccGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCggg                                                                                                                              >  2:3376104/1‑148 (MQ=255)
      accGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGc                                                                                                                             <  1:2856971/148‑1 (MQ=255)
      accGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGc                                                                                                                             <  1:672141/148‑1 (MQ=255)
      accGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGc                                                                                                                             <  2:5800/148‑1 (MQ=255)
      accGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGc                                                                                                                             <  2:1849276/148‑1 (MQ=255)
          aaGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTAcc                                                                                                                         >  2:2823029/1‑148 (MQ=255)
          aaGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTAcc                                                                                                                         >  2:73892/1‑148 (MQ=255)
          aaGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTAcc                                                                                                                         <  1:3008800/148‑1 (MQ=255)
               ttttACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACg                                                                                                                    >  1:3701067/1‑148 (MQ=255)
                  tACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCt                                                                                                                 <  1:2172294/148‑1 (MQ=255)
                   aCAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCtt                                                                                                                <  1:938695/148‑1 (MQ=255)
                   aCAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCtt                                                                                                                <  1:1067668/148‑1 (MQ=255)
                   aCAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCtt                                                                                                                <  1:3472053/148‑1 (MQ=255)
                      aCTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTgg                                                                                                             <  2:1980157/148‑1 (MQ=255)
                       cTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGt                                                                                                            <  1:2384761/148‑1 (MQ=255)
                           ttGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGt                                                                                                        >  1:2582779/1‑148 (MQ=255)
                           ttGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGt                                                                                                        >  1:664163/1‑148 (MQ=255)
                           ttGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGt                                                                                                        <  2:2492232/148‑1 (MQ=255)
                           ttGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGt                                                                                                        >  1:2850057/1‑148 (MQ=255)
                               gcCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGa                                                                                                    <  1:3680534/148‑1 (MQ=255)
                                ccACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGAt                                                                                                   <  1:3483213/148‑1 (MQ=255)
                                  aCGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGAtct                                                                                                 >  2:1024994/1‑148 (MQ=255)
                                  aCGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGAtct                                                                                                 >  2:2931290/1‑148 (MQ=255)
                                    gCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCg                                                                                               <  2:2278611/148‑1 (MQ=255)
                                       gAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACg                                                                                            <  2:2171370/148‑1 (MQ=255)
                                        aaGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGc                                                                                           <  1:3750471/148‑1 (MQ=255)
                                         aGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGcc                                                                                          >  1:2202234/1‑148 (MQ=255)
                                             ctGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGaca                                                                                      <  2:1471547/148‑1 (MQ=255)
                                              tGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGacac                                                                                     >  2:916205/1‑148 (MQ=255)
                                                aCCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGa                                                                                   <  2:2892196/148‑1 (MQ=255)
                                                   tttGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATgg                                                                                >  1:3752643/1‑148 (MQ=255)
                                                           gAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAg                                                                        >  1:1218609/1‑148 (MQ=255)
                                                           gAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAg                                                                        >  1:1218610/1‑148 (MQ=255)
                                                           gAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAg                                                                        >  1:3331106/1‑148 (MQ=255)
                                                            aaCTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTATGATCTCGACGCCGACACGATGGCAAAACAGa                                                                       >  2:2926232/1‑148 (MQ=255)
                                                            aaCTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGa                                                                       >  2:424577/1‑148 (MQ=255)
                                                            aaCTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGa                                                                       >  2:424580/1‑148 (MQ=255)
                                                            aaCTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGa                                                                       <  1:529922/148‑1 (MQ=255)
                                                             acggtgCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGAt                                                                      <  2:3738021/145‑1 (MQ=255)
                                                             aCTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGAt                                                                      <  1:3525985/148‑1 (MQ=255)
                                                             aCTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGAt                                                                      <  1:493532/148‑1 (MQ=255)
                                                              cTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATa                                                                     >  1:1437567/1‑148 (MQ=255)
                                                              cTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATa                                                                     >  1:1437548/1‑148 (MQ=255)
                                                              cTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATa                                                                     >  1:3294361/1‑148 (MQ=255)
                                                                gtgCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACg                                                                   <  2:1842837/148‑1 (MQ=255)
                                                                gtgCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACg                                                                   <  2:1521456/148‑1 (MQ=255)
                                                                  gcgcTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTg                                                                 <  1:388587/148‑1 (MQ=255)
                                                                    gcTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGaa                                                               <  1:1047765/148‑1 (MQ=255)
                                                                    gcTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGaa                                                               <  1:1728868/148‑1 (MQ=255)
                                                                      tGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATg                                                             <  1:2651908/148‑1 (MQ=255)
                                                                      tGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATg                                                             <  1:2651427/148‑1 (MQ=255)
                                                                        ggtcccGATTAACTGCGCCAGTACGTGCAGGATCTGTCACGGATTGCGGAATGCTACTTCTCCGCTCACCCGAACGCCCTGCTAACCAACGCCATAGGTGAGTACGATCTCTACGCCGACACGATGGCAAAACACATACGAGaaattg                                                           >  2:2499965/4‑144 (MQ=255)
                                                                        gggCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGACTggg                                                           >  2:1940823/1‑148 (MQ=255)
                                                                        gggCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATggg                                                           >  2:2452439/1‑148 (MQ=255)
                                                                            ccGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCa                                                       <  1:1950809/148‑1 (MQ=255)
                                                                             cGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCaa                                                      >  1:899019/1‑148 (MQ=255)
                                                                              gATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAg                                                     >  2:3126150/1‑148 (MQ=255)
                                                                                tGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCg                                                   >  2:1674687/1‑148 (MQ=255)
                                                                                tGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCg                                                   <  2:2110553/148‑1 (MQ=255)
                                                                                  aaCTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCggg                                                 <  2:1558412/148‑1 (MQ=255)
                                                                                  aaCTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCggg                                                 >  2:636785/1‑148 (MQ=255)
                                                                                    cTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGtt                                               <  1:185453/148‑1 (MQ=255)
                                                                                    cTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGtt                                               <  1:185474/148‑1 (MQ=255)
                                                                                    cTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGtt                                               <  2:491408/148‑1 (MQ=255)
                                                                                      gcgcCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGtttt                                             <  2:2942705/148‑1 (MQ=255)
                                                                                           aGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCaa                                        <  2:1070169/148‑1 (MQ=255)
                                                                                           aGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCaa                                        <  2:3698090/148‑1 (MQ=255)
                                                                                           aGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCaa                                        <  2:3704733/148‑1 (MQ=255)
                                                                                            gTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAat                                       <  1:1309572/148‑1 (MQ=255)
                                                                                              aCGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAatat                                     <  1:1442120/148‑1 (MQ=255)
                                                                                                 tGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATAtcgt                                  <  1:3055962/148‑1 (MQ=255)
                                                                                                 tGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATAtcgt                                  >  2:1326701/1‑148 (MQ=255)
                                                                                                       aGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTcggcgg                            <  1:2823029/148‑1 (MQ=255)
                                                                                                       aGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTcggcgg                            <  1:1013719/148‑1 (MQ=255)
                                                                                                       aGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTcggcgg                            <  1:1666439/148‑1 (MQ=255)
                                                                                                        gCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCggcggc                           >  1:1599570/1‑148 (MQ=255)
                                                                                                         cTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGgct                          >  1:1597641/1‑148 (MQ=255)
                                                                                                           gTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGgctgc                        >  1:3575635/1‑148 (MQ=255)
                                                                                                           gTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGgctgc                        >  2:1164153/1‑148 (MQ=255)
                                                                                                             cACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGctgctg                      >  1:58748/1‑148 (MQ=255)
                                                                                                             cACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGctgctg                      >  1:1993287/1‑148 (MQ=255)
                                                                                                             cACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGctgctg                      >  1:3250850/1‑148 (MQ=255)
                                                                                                             cACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGctgctg                      >  1:3250851/1‑148 (MQ=255)
                                                                                                              aCGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCtgt                     <  2:3129516/148‑1 (MQ=255)
                                                                                                                     gCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGcacca              <  2:1070551/148‑1 (MQ=255)
                                                                                                                        gAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGc           >  1:1523468/1‑148 (MQ=255)
                                                                                                                            gCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCcaca       <  2:3730386/148‑1 (MQ=255)
                                                                                                                            gCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCcaca       <  2:1066931/148‑1 (MQ=255)
                                                                                                                            gCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCcaca       <  2:680735/148‑1 (MQ=255)
                                                                                                                            gCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGCCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCcaca       <  2:3731301/148‑1 (MQ=255)
                                                                                                                              tACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCacaac     <  1:919436/148‑1 (MQ=255)
                                                                                                                              tACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCacaac     <  1:2832997/148‑1 (MQ=255)
                                                                                                                               aCGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCacaaca    >  1:128992/1‑148 (MQ=255)
                                                                                                                                cGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACat   >  2:1492984/1‑148 (MQ=255)
                                                                                                                                 gTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACata  <  2:2471465/148‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                                
CAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATA  >  CP009273/4214431‑4214707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATAT  >  CP009273/4214414‑4214708
                                                                                                                                                     |                                                                                                                                                 
GGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCT                                                                                                                                                   >  SRR19973069.3722260/1‑150 (MQ=60)
GGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCT                                                                                                                                                   >  SRR19973069.1077631/1‑150 (MQ=60)
 GCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTC                                                                                                                                                  <  SRR19973069.264544/150‑1 (MQ=60)
  CGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCC                                                                                                                                                 <  SRR19973069.101057/150‑1 (MQ=60)
                      CACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCT                                                                                                                             <  SRR19973069.655973/150‑1 (MQ=60)
                       ACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTA                                                                                                                            <  SRR19973069.2875767/150‑1 (MQ=60)
                       ACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTA                                                                                                                            <  SRR19973069.676763/150‑1 (MQ=60)
                           AAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCA                                                                                                                        <  SRR19973069.3028762/150‑1 (MQ=60)
                            AGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAA                                                                                                                       >  SRR19973069.3765954/1‑150 (MQ=60)
                              CATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACG                                                                                                                     >  SRR19973069.3725255/1‑150 (MQ=60)
                               ATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGC                                                                                                                    >  SRR19973069.1546883/1‑150 (MQ=60)
                                   TACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTT                                                                                                                <  SRR19973069.2187124/150‑1 (MQ=60)
                                    ACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTG                                                                                                               <  SRR19973069.3494505/150‑1 (MQ=60)
                                    ACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTG                                                                                                               <  SRR19973069.945042/150‑1 (MQ=60)
                                    ACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTG                                                                                                               <  SRR19973069.1075104/150‑1 (MQ=60)
                                        CTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGA                                                                                                           <  SRR19973069.2400927/150‑1 (MQ=60)
                                          CATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGT                                                                                                         >  SRR19973069.2868826/1‑150 (MQ=60)
                                          CATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGT                                                                                                         >  SRR19973069.668672/1‑150 (MQ=60)
                                          CATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGT                                                                                                         >  SRR19973069.2599957/1‑150 (MQ=60)
                                             TGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACG                                                                                                      <  SRR19973069.138968/150‑1 (MQ=60)
                                                GCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATC                                                                                                   <  SRR19973069.3704607/150‑1 (MQ=60)
                                                 CCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCT                                                                                                  <  SRR19973069.3821476/150‑1 (MQ=60)
                                                 CCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCT                                                                                                  <  SRR19973069.3505703/150‑1 (MQ=60)
                                                        GAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCC                                                                                           >  SRR19973069.2217179/1‑150 (MQ=60)
                                                        GAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCC                                                                                           >  SRR19973069.1124724/1‑150 (MQ=60)
                                                         AAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCG                                                                                          <  SRR19973069.3774888/150‑1 (MQ=60)
                                                            CTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACA                                                                                       >  SRR19973069.3282476/1‑150 (MQ=60)
                                                              CTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACG                                                                                     >  SRR19973069.3087083/1‑150 (MQ=60)
                                                              CTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACG                                                                                     <  SRR19973069.2347508/150‑1 (MQ=60)
                                                                  CCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGG                                                                                 >  SRR19973069.3777063/1‑150 (MQ=60)
                                                                   CTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGC                                                                                >  SRR19973069.2026442/1‑150 (MQ=60)
                                                                   CTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGC                                                                                >  SRR19973069.99573/1‑150 (MQ=60)
                                                                          CTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAG                                                                         >  SRR19973069.1226949/1‑150 (MQ=60)
                                                                          CTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAG                                                                         >  SRR19973069.1226950/1‑150 (MQ=60)
                                                                          CTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAG                                                                         >  SRR19973069.3352931/1‑150 (MQ=60)
                                                                             AACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATA                                                                      <  SRR19973069.533661/150‑1 (MQ=60)
                                                                             AACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATA                                                                      >  SRR19973069.1447581/1‑150 (MQ=60)
                                                                             AACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATA                                                                      >  SRR19973069.3315932/1‑150 (MQ=60)
                                                                             AACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATA                                                                      >  SRR19973069.1447600/1‑150 (MQ=60)
                                                                             AACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATA                                                                      >  SRR19973069.1447815/1‑150 (MQ=60)
                                                                             AACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATA                                                                      <  SRR19973069.533620/150‑1 (MQ=60)
                                                                              ACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATAC                                                                     <  SRR19973069.497079/150‑1 (MQ=60)
                                                                              ACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATAC                                                                     <  SRR19973069.3548790/150‑1 (MQ=60)
                                                                               CTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACG                                                                    <  SRR19973069.354605/150‑1 (MQ=60)
                                                                                   GCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAA                                                                <  SRR19973069.391435/150‑1 (MQ=60)
                                                                                     GCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATG                                                              <  SRR19973069.1740982/150‑1 (MQ=60)
                                                                                     GCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATG                                                              <  SRR19973069.1055039/150‑1 (MQ=60)
                                                                                       TGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGG                                                            <  SRR19973069.2669190/150‑1 (MQ=60)
                                                                                       TGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGG                                                            <  SRR19973069.2669672/150‑1 (MQ=60)
                                                                                            CCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAA                                                       >  SRR19973069.905205/1‑150 (MQ=60)
                                                                                             CCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAG                                                      <  SRR19973069.1964296/150‑1 (MQ=60)
                                                                                              CGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGC                                                     >  SRR19973069.2773806/1‑150 (MQ=60)
                                                                                              CGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGC                                                     >  SRR19973069.1274113/1‑150 (MQ=60)
                                                                                                    ACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTT                                               >  SRR19973069.2636797/1‑150 (MQ=60)
                                                                                                     CTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTT                                              <  SRR19973069.186985/150‑1 (MQ=60)
                                                                                                     CTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTT                                              <  SRR19973069.187006/150‑1 (MQ=60)
                                                                                                     CTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTT                                              <  SRR19973069.187038/150‑1 (MQ=60)
                                                                                                     CTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTT                                              <  SRR19973069.1661167/150‑1 (MQ=60)
                                                                                                            AGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATA                                       >  SRR19973069.290696/1‑150 (MQ=60)
                                                                                                             GTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATAT                                      <  SRR19973069.1318523/150‑1 (MQ=60)
                                                                                                               ACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCG                                    <  SRR19973069.1452183/150‑1 (MQ=60)
                                                                                                               ACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCG                                    <  SRR19973069.3368972/150‑1 (MQ=60)
                                                                                                                  TGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCG                                 <  SRR19973069.3076179/150‑1 (MQ=60)
                                                                                                                       GAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGC                            >  SRR19973069.1610356/1‑150 (MQ=60)
                                                                                                                        AGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCT                           <  SRR19973069.2841701/150‑1 (MQ=60)
                                                                                                                        AGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCT                           <  SRR19973069.1020708/150‑1 (MQ=60)
                                                                                                                        AGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCT                           >  SRR19973069.1608421/1‑150 (MQ=60)
                                                                                                                        AGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCT                           <  SRR19973069.1677994/150‑1 (MQ=60)
                                                                                                                          CTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGC                         >  SRR19973069.3598945/1‑150 (MQ=60)
                                                                                                                            GTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTG                       >  SRR19973069.2007176/1‑150 (MQ=60)
                                                                                                                            GTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTG                       >  SRR19973069.59457/1‑150 (MQ=60)
                                                                                                                            GTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTG                       >  SRR19973069.3272132/1‑150 (MQ=60)
                                                                                                                            GTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTG                       >  SRR19973069.3272133/1‑150 (MQ=60)
                                                                                                                                       CGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGC            >  SRR19973069.1533965/1‑150 (MQ=60)
                                                                                                                                         GAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCA          <  SRR19973069.1763449/150‑1 (MQ=60)
                                                                                                                                         GAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCA          >  SRR19973069.687849/1‑150 (MQ=60)
                                                                                                                                              CTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACA     >  SRR19973069.130202/1‑150 (MQ=60)
                                                                                                                                              CTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACA     <  SRR19973069.3692490/150‑1 (MQ=60)
                                                                                                                                               TACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACAT    <  SRR19973069.2851708/150‑1 (MQ=60)
                                                                                                                                               TACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACAT    <  SRR19973069.925705/150‑1 (MQ=60)
                                                                                                                                                ACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATA   <  SRR19973069.285627/150‑1 (MQ=60)
                                                                                                                                                 CGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATAT  >  SRR19973069.2700560/1‑150 (MQ=60)
                                                                                                                                                 CGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATAT  >  SRR19973069.770885/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                 
GGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATAT  >  CP009273/4214414‑4214708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑