Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F527 I0 R1	1651	322.4	10661268	95.2%	10149527	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,214,563	A→T	100%	T270S (ACC→TCC)	metH →	homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,214,563	0	A	T	99.6%	898.2 / ‑4.7	269	T270S (ACC→TCC)	metH	homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (120/148); total (120/149)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 269 total aligned reads displayed.

CGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATT  >  CP009273/4214416‑4214709

cgcACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTcc                                                                                                                                                  <  1:2261686/150‑1 (MQ=255)

cgcACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTcc                                                                                                                                                  <  1:730243/150‑1 (MQ=255)

cgcACGCTCTCCGGGCAGACCAACGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTTGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTcc                                                                                                                                                  <  2:3540817/150‑1 (MQ=255)

 gcACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGACACGGATTGCGGAATGCTACGTCTCcg                                                                                                                                                 >  1:602293/1‑150 (MQ=255)

  cACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCcgc                                                                                                                                                >  1:1200253/1‑150 (MQ=255)

        ctcCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCAccc                                                                                                                                          >  2:5044626/1‑150 (MQ=255)

            gggCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAAc                                                                                                                                      <  2:304353/150‑1 (MQ=255)

            gggCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAAc                                                                                                                                      >  1:2844660/1‑150 (MQ=255)

             ggCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACg                                                                                                                                     <  1:3994281/150‑1 (MQ=255)

              gCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGc                                                                                                                                    <  2:3894183/150‑1 (MQ=255)

               cAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGcc                                                                                                                                   <  2:2993169/150‑1 (MQ=255)

                aGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCg                                                                                                                                  <  2:2352764/150‑1 (MQ=255)

                    caccGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCt                                                                                                                              <  2:2982287/150‑1 (MQ=255)

                       cGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTAcc                                                                                                                           >  2:264531/1‑150 (MQ=255)

                       cGAAGCATTTTACAACTCAGTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACGGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGACCGCCGGGCTAcc                                                                                                                           <  2:459695/150‑1 (MQ=255)

                         aggCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCa                                                                                                                         >  1:2666180/3‑150 (MQ=255)

                          aGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCaa                                                                                                                        >  1:3819978/1‑150 (MQ=255)

                          aGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCaa                                                                                                                        >  1:3484998/1‑150 (MQ=255)

                              ttttACAACTCNTTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGcc                                                                                                                    >  1:2762649/1‑150 (MQ=255)

                                     aCTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTg                                                                                                             <  2:2881727/150‑1 (MQ=255)

                                      cTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGa                                                                                                            >  1:4762380/1‑150 (MQ=255)

                                          ttGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTAc                                                                                                        <  2:2271282/150‑1 (MQ=255)

                                              gcCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGAtc                                                                                                    >  2:4814071/1‑150 (MQ=255)

                                              gcCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGAtc                                                                                                    <  1:5178677/150‑1 (MQ=255)

                                              gcCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGAtc                                                                                                    >  2:3935955/1‑150 (MQ=255)

                                               ccACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGAtct                                                                                                   <  1:3316325/150‑1 (MQ=255)

                                                cACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGAtctc                                                                                                  <  2:4718593/150‑1 (MQ=255)

                                                 aCGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGAtctca                                                                                                 <  2:4979324/150‑2 (MQ=255)

                                                   gCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGAc                                                                                               <  2:3681181/150‑1 (MQ=255)

                                                   gCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGAc                                                                                               <  2:2507434/150‑1 (MQ=255)

                                                    ccGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACACCGGGCTACCCAACGCCTTTGGTGAGTCCGATCTCGACg                                                                                              <  1:4904560/150‑1 (MQ=255)

                                                     cGAAGCTCTGACCTTTGGACTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAAAGCTACGTCTCAGCGCACCCGAACGCCGGGCTACACAACGCCTTTGGTGAGTACGATCTCGACGc                                                                                             >  2:4911627/1‑150 (MQ=255)

                                                       aaGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGACTGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCg                                                                                           >  2:1061043/1‑150 (MQ=255)

                                                       aaGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCg                                                                                           >  1:2459958/1‑150 (MQ=255)

                                                       aaGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCg                                                                                           >  1:4816784/1‑150 (MQ=255)

                                                        aGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGa                                                                                          >  1:3055353/1‑150 (MQ=255)

                                                          ctctGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGaca                                                                                        <  2:2199055/150‑1 (MQ=255)

                                                          ctctGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGaca                                                                                        <  1:2613362/150‑1 (MQ=255)

                                                             tGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGa                                                                                     >  2:1505252/1‑150 (MQ=255)

                                                              gACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGAt                                                                                    <  2:4323845/150‑1 (MQ=255)

                                                              gACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGAt                                                                                    <  2:2110056/150‑1 (MQ=255)

                                                              gACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGAt                                                                                    <  2:3057678/150‑1 (MQ=255)

                                                                 cTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGc                                                                                 <  2:5329722/150‑1 (MQ=255)

                                                                    tGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCaaa                                                                              >  2:5104893/1‑150 (MQ=255)

                                                                    tGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCaaa                                                                              >  2:1987429/1‑150 (MQ=255)

                                                                         tGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGa                                                                         <  2:625609/150‑1 (MQ=255)

                                                                         tGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGa                                                                         >  1:357141/1‑150 (MQ=255)

                                                                          gAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGAt                                                                        >  2:3844688/1‑150 (MQ=255)

                                                                           aaCTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATa                                                                       >  1:2257770/1‑150 (MQ=255)

                                                                              tgtgCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGt                                                                    <  1:4164175/150‑1 (MQ=255)

                                                                                  cgcTGGGGCCCGATGAACTGCGACAGTTCGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAAt                                                                <  2:1115136/150‑1 (MQ=255)

                                                                                   gcTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCTGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTTCGATATCGACGCCGACACGATGGCAAAACAGATACGTGATTg                                                               <  1:2116890/150‑1 (MQ=255)

                                                                                    cTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATgg                                                              <  1:3029033/150‑1 (MQ=255)

                                                                                        ggCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGgcgc                                                          <  2:4627644/150‑1 (MQ=255)

                                                                                        ggCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGgcgc                                                          <  1:5267002/150‑1 (MQ=255)

                                                                                            cGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGc                                                      <  1:4552836/150‑1 (MQ=255)

                                                                                            cGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGc                                                      >  2:1774936/1‑150 (MQ=255)

                                                                                              aTGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCgg                                                    <  1:3222344/150‑1 (MQ=255)

                                                                                              aTGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCgg                                                    <  1:2695279/150‑1 (MQ=255)

                                                                                                gAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGt                                                  >  2:4115043/1‑150 (MQ=255)

                                                                                                gAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGt                                                  <  1:1454260/150‑1 (MQ=255)

                                                                                                gAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGt                                                  >  1:135329/1‑150 (MQ=255)

                                                                                                  aCTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGttt                                                <  2:3371680/150‑1 (MQ=255)

                                                                                                     gcgcCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTtct                                             <  2:166358/150‑1 (MQ=255)

                                                                                                     gcgcCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTtct                                             <  1:4030963/150‑1 (MQ=255)

                                                                                                     gcgcCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTtct                                             <  1:2005872/150‑1 (MQ=255)

                                                                                                         cAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAat                                         <  2:3954939/150‑1 (MQ=255)

                                                                                                         cAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAat                                         <  2:4442640/150‑1 (MQ=255)

                                                                                                           gTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAatat                                       <  1:605996/150‑1 (MQ=255)

                                                                                                            tACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATAtc                                      <  1:1291768/150‑1 (MQ=255)

                                                                                                            tACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATAtc                                      <  1:2125259/150‑1 (MQ=255)

                                                                                                               gTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATAtcgtc                                   >  1:989549/1‑150 (MQ=255)

                                                                                                               gTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATAtcgtc                                   >  2:236121/1‑150 (MQ=255)

                                                                                                                 gCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTcgg                                 <  1:1730388/150‑1 (MQ=255)

                                                                                                                 gCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTcgg                                 <  1:1916658/150‑1 (MQ=255)

                                                                                                                  cAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTcggc                                <  1:2055271/150‑1 (MQ=255)

                                                                                                                   aGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTcggcg                               >  1:2801558/1‑150 (MQ=255)

                                                                                                                   aGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTcggcg                               >  1:4243914/1‑150 (MQ=255)

                                                                                                                    ggAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTcggcgg                              >  2:4128224/1‑150 (MQ=255)

                                                                                                                       gCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGgctg                           >  1:487237/1‑150 (MQ=255)

                                                                                                                          gTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGctgctg                        >  2:5155997/1‑150 (MQ=255)

                                                                                                                          gTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGctgctg                        >  1:3910861/1‑150 (MQ=255)

                                                                                                                           tCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCtgt                       <  1:2183481/150‑1 (MQ=255)

                                                                                                                              cGGATTGAGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGCTACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGc                    <  1:4638778/150‑1 (MQ=255)

                                                                                                                               ggATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGca                   <  2:3672621/150‑1 (MQ=255)

                                                                                                                               ggATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGca                   >  1:2949608/1‑150 (MQ=255)

                                                                                                                                      ggAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGcc            >  2:3276976/1‑150 (MQ=255)

                                                                                                                                       gAATGCTACGTCTCCGCGCACCCGACCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATCCGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCca           <  1:813016/150‑1 (MQ=255)

                                                                                                                                       gAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCca           >  1:4998776/1‑150 (MQ=255)

                                                                                                                                       gAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCca           >  1:5090802/1‑150 (MQ=255)

                                                                                                                                       gAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCATTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCTTCGGCGGATGCTGTGTCACCACGCca           >  1:4399169/1‑150 (MQ=255)

                                                                                                                                        aaTGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCcac          >  2:618626/1‑150 (MQ=255)

                                                                                                                                         aTGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCcaca         <  2:117496/150‑1 (MQ=255)

                                                                                                                                            cTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCACTATCGTCGGCGGCTGCTGTGGCACCACGCCacaaca      <  2:4240453/150‑1 (MQ=255)

                                                                                                                                            cTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCacaaca      >  2:4740244/1‑150 (MQ=255)

                                                                                                                                            cTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCacaaca      >  2:3171824/1‑150 (MQ=255)

                                                                                                                                             tACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCGTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCacaacag     >  2:5244034/1‑149 (MQ=255)

                                                                                                                                              aCGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACata    >  1:1636442/1‑150 (MQ=255)

                                                                                                                                               cGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACatat   >  2:767533/1‑150 (MQ=255)

                                                                                                                                                gTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATAtt  >  2:3076335/1‑150 (MQ=255)

CGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATT  >  CP009273/4214416‑4214709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGC  >  CP009273/4214416‑4214711

CGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCC                                                                                                                                                    <  SRR19973062.2273110/150‑1 (MQ=60)

CGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCC                                                                                                                                                    <  SRR19973062.1319928/150‑1 (MQ=60)

CGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCC                                                                                                                                                    <  SRR19973062.3175987/150‑1 (MQ=60)

CGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCC                                                                                                                                                    <  SRR19973062.733569/150‑1 (MQ=60)

 GCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGACACGGATTGCGGAATGCTACGTCTCCG                                                                                                                                                   >  SRR19973062.605110/1‑150 (MQ=60)

  CACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGC                                                                                                                                                  >  SRR19973062.1205854/1‑150 (MQ=60)

  CACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGC                                                                                                                                                  >  SRR19973062.2784094/1‑150 (MQ=60)

            GGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAAC                                                                                                                                        <  SRR19973062.2512185/150‑1 (MQ=60)

            GGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAAC                                                                                                                                        <  SRR19973062.4797812/150‑1 (MQ=60)

            GGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAAC                                                                                                                                        >  SRR19973062.2859704/1‑150 (MQ=60)

             GGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACG                                                                                                                                       <  SRR19973062.4014321/150‑1 (MQ=60)

             GGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACG                                                                                                                                       <  SRR19973062.4014456/150‑1 (MQ=60)

               CAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCC                                                                                                                                     >  SRR19973062.1328326/1‑150 (MQ=60)

                AGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCG                                                                                                                                    >  SRR19973062.2185781/1‑150 (MQ=60)

                AGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCG                                                                                                                                    >  SRR19973062.3030741/1‑150 (MQ=60)

                    CACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCT                                                                                                                                <  SRR19973062.5062736/150‑1 (MQ=60)

                     ACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTA                                                                                                                               <  SRR19973062.1284114/150‑1 (MQ=60)

                     ACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTA                                                                                                                               <  SRR19973062.2238504/150‑1 (MQ=60)

                         AGGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCA                                                                                                                           >  SRR19973062.2680168/1‑150 (MQ=60)

                          AGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAA                                                                                                                          >  SRR19973062.3502706/1‑150 (MQ=60)

                          AGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAA                                                                                                                          >  SRR19973062.3839514/1‑150 (MQ=60)

                              TTTTACAACTCNTTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCC                                                                                                                      >  SRR19973062.2777243/1‑150 (MQ=60)

                                 TACAACTCATGGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTT                                                                                                                   <  SRR19973062.767368/150‑1 (MQ=60)

                                    AACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGT                                                                                                                >  SRR19973062.5273651/1‑150 (MQ=60)

                                      CTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGA                                                                                                              >  SRR19973062.4787140/1‑150 (MQ=60)

                                      CTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGA                                                                                                              >  SRR19973062.972987/1‑150 (MQ=60)

                                          TTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTAC                                                                                                          <  SRR19973062.5019641/150‑1 (MQ=60)

                                          TTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTAC                                                                                                          <  SRR19973062.3688073/150‑1 (MQ=60)

                                          TTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTAC                                                                                                          <  SRR19973062.5337772/150‑1 (MQ=60)

                                             CGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGAT                                                                                                       >  SRR19973062.364582/1‑150 (MQ=60)

                                              GCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATC                                                                                                      <  SRR19973062.5206021/150‑1 (MQ=60)

                                               CCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCT                                                                                                     <  SRR19973062.3333459/150‑1 (MQ=60)

                                               CCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCT                                                                                                     <  SRR19973062.3333471/150‑1 (MQ=60)

                                               CCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCT                                                                                                     <  SRR19973062.4017093/150‑1 (MQ=60)

                                               CCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCT                                                                                                     <  SRR19973062.2106110/150‑1 (MQ=60)

                                                CACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTC                                                                                                    <  SRR19973062.2680046/150‑1 (MQ=60)

                                                 ACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCG                                                                                                   <  SRR19973062.722793/150‑1 (MQ=60)

                                                 ACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCG                                                                                                   <  SRR19973062.722794/150‑1 (MQ=60)

                                                   GCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGAC                                                                                                 <  SRR19973062.4744578/150‑1 (MQ=60)

                                                   GCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGAC                                                                                                 <  SRR19973062.737575/150‑1 (MQ=60)

                                                    CCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACG                                                                                                <  SRR19973062.2899626/150‑1 (MQ=60)

                                                    CCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACG                                                                                                <  SRR19973062.4931228/150‑1 (MQ=60)

                                                    CCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACACCGGGCTACCCAACGCCTTTGGTGAGTCCGATCTCGACG                                                                                                <  SRR19973062.4929883/150‑1 (MQ=60)

                                                      GAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCC                                                                                              >  SRR19973062.559311/1‑150 (MQ=60)

                                                      GAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCC                                                                                              >  SRR19973062.2709066/1‑150 (MQ=60)

                                                       AAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCG                                                                                             >  SRR19973062.2473153/1‑150 (MQ=60)

                                                       AAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCG                                                                                             >  SRR19973062.4841732/1‑150 (MQ=60)

                                                        AGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGA                                                                                            >  SRR19973062.3071221/1‑150 (MQ=60)

                                                          CTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACA                                                                                          >  SRR19973062.3572833/1‑150 (MQ=60)

                                                          CTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACA                                                                                          <  SRR19973062.2627164/150‑1 (MQ=60)

                                                          CTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACA                                                                                          >  SRR19973062.3572733/1‑150 (MQ=60)

                                                             TGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGA                                                                                       <  SRR19973062.265855/150‑1 (MQ=60)

                                                             TGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGA                                                                                       <  SRR19973062.4427357/150‑1 (MQ=60)

                                                             TGACCTTTGGCCTGAACTGTGCGATGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGA                                                                                       <  SRR19973062.1939925/150‑1 (MQ=60)

                                                              GACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGAT                                                                                      <  SRR19973062.4859679/150‑1 (MQ=60)

                                                               ACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATG                                                                                     <  SRR19973062.5264490/150‑1 (MQ=60)

                                                               ACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATG                                                                                     <  SRR19973062.3306131/150‑1 (MQ=60)

                                                                 CTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGC                                                                                   >  SRR19973062.1394903/1‑150 (MQ=60)

                                                                 CTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGC                                                                                   >  SRR19973062.1394975/1‑150 (MQ=60)

                                                                     GGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAA                                                                               <  SRR19973062.1647606/150‑1 (MQ=60)

                                                                      GCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAAC                                                                              <  SRR19973062.4692265/150‑1 (MQ=60)

                                                                      GCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAAC                                                                              >  SRR19973062.1836652/1‑150 (MQ=60)

                                                                      GCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAAC                                                                              <  SRR19973062.271861/150‑1 (MQ=60)

                                                                         TGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGA                                                                           >  SRR19973062.358757/1‑150 (MQ=60)

                                                                           AACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATA                                                                         >  SRR19973062.2269106/1‑150 (MQ=60)

                                                                           AACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATA                                                                         >  SRR19973062.2269258/1‑150 (MQ=60)

                                                                             CTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACG                                                                       >  SRR19973062.292018/1‑150 (MQ=60)

                                                                              TGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGT                                                                      <  SRR19973062.4185780/150‑1 (MQ=60)

                                                                                   GCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCTGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTTCGATATCGACGCCGACACGATGGCAAAACAGATACGTGATTG                                                                 <  SRR19973062.2127661/150‑1 (MQ=60)

                                                                                   GCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAcgg                                                                 >  SRR19973062.2053246/1‑147 (MQ=60)

                                                                                   GCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATG                                                                 <  SRR19973062.2127744/150‑1 (MQ=60)

                                                                                   GCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATG                                                                 <  SRR19973062.4793131/150‑1 (MQ=60)

                                                                                    CTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGG                                                                <  SRR19973062.3374551/150‑1 (MQ=60)

                                                                                    CTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGG                                                                <  SRR19973062.3044824/150‑1 (MQ=60)

                                                                                     TGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGG                                                               <  SRR19973062.2459801/150‑1 (MQ=60)

                                                                                     TGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGG                                                               <  SRR19973062.5119808/150‑1 (MQ=60)

                                                                                        GGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGC                                                            <  SRR19973062.5294709/150‑1 (MQ=60)

                                                                                        GGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGC                                                            <  SRR19973062.4075555/150‑1 (MQ=60)

                                                                                        GGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGC                                                            <  SRR19973062.4044658/150‑1 (MQ=60)

                                                                                          CCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAA                                                          <  SRR19973062.2515235/150‑1 (MQ=60)

                                                                                           CCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAG                                                         >  SRR19973062.2785576/1‑150 (MQ=60)

                                                                                            CGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGC                                                        <  SRR19973062.4576588/150‑1 (MQ=60)

                                                                                              ATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGG                                                      <  SRR19973062.2709396/150‑1 (MQ=60)

                                                                                              ATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGG                                                      >  SRR19973062.1821135/1‑150 (MQ=60)

                                                                                              ATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGG                                                      <  SRR19973062.3239074/150‑1 (MQ=60)

                                                                                                GAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGGACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGT                                                    >  SRR19973062.1706789/1‑150 (MQ=60)

                                                                                                GAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGT                                                    <  SRR19973062.2225629/150‑1 (MQ=60)

                                                                                                GAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGT                                                    >  SRR19973062.136135/1‑150 (MQ=60)

                                                                                                GAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGT                                                    <  SRR19973062.1461359/150‑1 (MQ=60)

                                                                                                GAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGT                                                    <  SRR19973062.4927219/150‑1 (MQ=60)

                                                                                                 AACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCATCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTT                                                   >  SRR19973062.3041312/1‑150 (MQ=60)

                                                                                                    TGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTC                                                <  SRR19973062.2033303/150‑1 (MQ=60)

                                                                                                     GCGCCAGTACGTTCATGAGCTGTCACTGATTGCGTAATGCTACGTCTCCGCGCACCCGAACGCCGTGATACCCAACGACTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCT                                               <  SRR19973062.2076439/150‑1 (MQ=60)

                                                                                                     GCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCT                                               <  SRR19973062.4051462/150‑1 (MQ=60)

                                                                                                     GCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCT                                               <  SRR19973062.2016182/150‑1 (MQ=60)

                                                                                                           GTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATAT                                         <  SRR19973062.608830/150‑1 (MQ=60)

                                                                                                           GTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATAT                                         <  SRR19973062.4406070/150‑1 (MQ=60)

                                                                                                            TACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATC                                        <  SRR19973062.1482877/150‑1 (MQ=60)

                                                                                                            TACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATC                                        <  SRR19973062.2136059/150‑1 (MQ=60)

                                                                                                            TACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATC                                        <  SRR19973062.1297690/150‑1 (MQ=60)

                                                                                                            TACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATC                                        <  SRR19973062.2136117/150‑1 (MQ=60)

                                                                                                               GTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTC                                     >  SRR19973062.994148/1‑150 (MQ=60)

                                                                                                               GTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTC                                     <  SRR19973062.2762764/150‑1 (MQ=60)

                                                                                                               GTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTC                                     <  SRR19973062.38681/150‑1 (MQ=60)

                                                                                                               GTGCAGGAGCTGTCACGGAGTGCGGAAGGCTACGTCTCCGCGCACCCGCACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCTAAACAGATACGTGAAGGGGCGCAAGCGGGTTTTCTCAATATCGTC                                     <  SRR19973062.2360706/150‑1 (MQ=60)

                                                                                                                TGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCG                                    <  SRR19973062.3232658/150‑1 (MQ=60)

                                                                                                                 GCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGG                                   <  SRR19973062.1738815/150‑1 (MQ=60)

                                                                                                                 GCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGG                                   <  SRR19973062.1926468/150‑1 (MQ=60)

                                                                                                                  CAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGC                                  <  SRR19973062.2065793/150‑1 (MQ=60)

                                                                                                                   AGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCG                                 >  SRR19973062.2816624/1‑150 (MQ=60)

                                                                                                                   AGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCG                                 >  SRR19973062.2816375/1‑150 (MQ=60)

                                                                                                                   AGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCG                                 >  SRR19973062.4265891/1‑150 (MQ=60)

                                                                                                                   AGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCG                                 <  SRR19973062.4649892/150‑1 (MQ=60)

                                                                                                                   AGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCG                                 >  SRR19973062.3870417/1‑150 (MQ=60)

                                                                                                                    GGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGG                                >  SRR19973062.3046086/1‑150 (MQ=60)

                                                                                                                       GCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTG                             >  SRR19973062.489481/1‑150 (MQ=60)

                                                                                                                         TGTCACGGATGGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATCCGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCT                           <  SRR19973062.4368586/150‑1 (MQ=60)

                                                                                                                          GTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTG                          >  SRR19973062.3930776/1‑150 (MQ=60)

                                                                                                                          GTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTG                          <  SRR19973062.410815/150‑1 (MQ=60)

                                                                                                                          GTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTG                          >  SRR19973062.608824/1‑150 (MQ=60)

                                                                                                                           TCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGT                         <  SRR19973062.2194466/150‑1 (MQ=60)

                                                                                                                              CGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGC                      >  SRR19973062.4923527/1‑150 (MQ=60)

                                                                                                                              CGGATTGAGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGCTACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGC                      <  SRR19973062.4663047/150‑1 (MQ=60)

                                                                                                                               GGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCA                     >  SRR19973062.2965109/1‑150 (MQ=60)

                                                                                                                                       GAATGCTACGTCTCCGCGCACCCGACCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATCCGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCA             <  SRR19973062.816607/150‑1 (MQ=60)

                                                                                                                                       GAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCA             >  SRR19973062.4421807/1‑150 (MQ=60)

                                                                                                                                       GAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCA             >  SRR19973062.5117776/1‑150 (MQ=60)

                                                                                                                                       GAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCA             >  SRR19973062.5117750/1‑150 (MQ=60)

                                                                                                                                       GAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCA             >  SRR19973062.4253733/1‑150 (MQ=60)

                                                                                                                                       GAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCA             >  SRR19973062.5025198/1‑150 (MQ=60)

                                                                                                                                       GAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCATTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCTTCGGCGGATGCTGTGTCACCACGCCA             >  SRR19973062.4421687/1‑150 (MQ=60)

                                                                                                                                              ACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATA      >  SRR19973062.1644513/1‑150 (MQ=60)

                                                                                                                                                  CTCCGCGCACCCGAACGCCGGGCTACCCAACGCCGTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGC  >  SRR19973062.137445/1‑150 (MQ=60)

CGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGC  >  CP009273/4214416‑4214711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`