Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 185,393 A→T 100% F201I (TTC→ATC)  map ← methionine aminopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273185,3930AT99.7% 932.1 / ‑4.8 284F201I (TTC→ATC) mapmethionine aminopeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (142/141);  total (142/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 284 total aligned reads displayed.

GCGTCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCG  >  CP009273/185246‑185539
                                                                                                                                                   |                                                                                                                                                  
        agttCGCAGCCGTTATCAGTCACCACAATAGTAGGCTCATATTGTGCAGTCAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGAGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCa                                                                                                                                          <  2:160724/148‑1 (MQ=255)
        aTTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTTCCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCa                                                                                                                                          >  1:3655985/1‑150 (MQ=255)
        aTTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCa                                                                                                                                          <  2:4082723/150‑1 (MQ=255)
         tttCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAg                                                                                                                                         >  1:2242513/1‑150 (MQ=255)
          ttCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTTACCATTTGCTCGATGGTTATCGTCATCCCAgg                                                                                                                                        >  1:938665/1‑150 (MQ=255)
          ttCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAgg                                                                                                                                        <  1:799647/150‑1 (MQ=255)
           tCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGt                                                                                                                                       <  2:980432/150‑1 (MQ=255)
            cGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTATTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATAGTCATCCCAGGtt                                                                                                                                      >  1:4995111/1‑150 (MQ=255)
             gCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGttt                                                                                                                                     <  2:2529719/150‑1 (MQ=255)
               aGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCa                                                                                                                                   >  1:4582253/1‑150 (MQ=255)
                  cGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTa                                                                                                                                >  1:4338181/1‑150 (MQ=255)
                   gTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTac                                                                                                                               <  1:3899822/150‑1 (MQ=255)
                   gTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTac                                                                                                                               <  2:2085306/150‑1 (MQ=255)
                      aTCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTacgac                                                                                                                            <  2:350447/150‑1 (MQ=255)
                      aTCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTacgac                                                                                                                            <  2:2039956/150‑1 (MQ=255)
                              ccacAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGttt                                                                                                                    <  2:3283690/150‑1 (MQ=255)
                               cacaATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTc                                                                                                                   >  2:4700830/1‑150 (MQ=255)
                                  aaTAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGTTGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACg                                                                                                                <  1:2544328/150‑1 (MQ=255)
                                  aaTAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACg                                                                                                                <  2:2007656/150‑1 (MQ=255)
                                     aGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGa                                                                                                             >  1:4589596/1‑150 (MQ=255)
                                          gCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCAt                                                                                                        <  2:2752110/150‑1 (MQ=255)
                                          gCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCAt                                                                                                        <  1:2478753/150‑1 (MQ=255)
                                            tCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAg                                                                                                      <  1:33733/150‑1 (MQ=255)
                                             cATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGt                                                                                                     <  2:2850919/150‑1 (MQ=255)
                                                atTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgca                                                                                                  <  2:3608167/150‑1 (MQ=255)
                                                atTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgca                                                                                                  >  1:1658234/1‑150 (MQ=255)
                                                   gtgCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgcagca                                                                                               >  1:4186075/1‑150 (MQ=255)
                                                      cAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCt                                                                                            <  2:167397/150‑1 (MQ=255)
                                                      cAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCt                                                                                            <  2:1342500/150‑1 (MQ=255)
                                                       aGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTg                                                                                           >  1:5258718/1‑150 (MQ=255)
                                                        gACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGc                                                                                          <  1:3693474/150‑1 (MQ=255)
                                                        gACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGc                                                                                          >  2:2524790/1‑150 (MQ=255)
                                                         aCAAGCTGCGATCTTAGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCg                                                                                         >  2:1791442/1‑150 (MQ=255)
                                                          cAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCgg                                                                                        >  2:705841/1‑150 (MQ=255)
                                                           aaGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGt                                                                                       >  1:4666005/1‑150 (MQ=255)
                                                             gCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGttc                                                                                     >  2:5012680/1‑150 (MQ=255)
                                                               tGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGttctt                                                                                   <  1:2831792/150‑1 (MQ=255)
                                                               tGCGATCGTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGAACTCTTTTTTACCCGCGTTGACCATTGGCTCGAGGGTGATCGTCATCCCAGGTTTCAGTACGACGATGGTTACACGGGAGTCAGAGTGCAGCACCTGCGGttcta                                                                                   >  2:540732/1‑149 (MQ=255)
                                                                gCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCCTGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGttcttc                                                                                  <  2:4408567/150‑1 (MQ=255)
                                                                 cGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCa                                                                                 >  1:1027421/1‑150 (MQ=255)
                                                                 cGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCa                                                                                 <  1:1886277/150‑1 (MQ=255)
                                                                    tCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTAAGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCTTgg                                                                              <  1:5172910/150‑1 (MQ=255)
                                                                     cTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGa                                                                             <  2:3040222/150‑1 (MQ=255)
                                                                      tttGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTAGATGGTGATCGTCATCCCATGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGaa                                                                            >  2:1213392/1‑150 (MQ=255)
                                                                          gTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCcg                                                                        <  2:4149123/150‑1 (MQ=255)
                                                                             tttACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGa                                                                     >  1:4047149/1‑150 (MQ=255)
                                                                               tACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGAcc                                                                   >  2:3739369/1‑150 (MQ=255)
                                                                               tACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGAcc                                                                   >  2:918903/1‑150 (MQ=255)
                                                                                aCCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCa                                                                  >  1:4766672/1‑150 (MQ=255)
                                                                                aCCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCa                                                                  >  2:3454165/1‑150 (MQ=255)
                                                                                  cGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAAt                                                                >  1:216425/1‑150 (MQ=255)
                                                                                       aGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCgt                                                           <  1:3959552/150‑1 (MQ=255)
                                                                                       aGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCgt                                                           >  1:1573160/1‑150 (MQ=255)
                                                                                         ccATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCgtgt                                                         >  2:404492/1‑150 (MQ=255)
                                                                                         ccATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCgtgt                                                         <  1:3569879/150‑1 (MQ=255)
                                                                                          cATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATCGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTc                                                        >  2:3528/1‑150 (MQ=255)
                                                                                          cATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTc                                                        <  2:768999/150‑1 (MQ=255)
                                                                                          cATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTc                                                        <  1:4464897/150‑1 (MQ=255)
                                                                                          cATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTc                                                        <  1:1791442/150‑1 (MQ=255)
                                                                                           aTCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTcc                                                       <  1:2533310/150‑1 (MQ=255)
                                                                                           aTCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTcc                                                       >  1:5318718/1‑150 (MQ=255)
                                                                                            tCTTTCATGGTGCGGATCTATTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCg                                                      <  2:2461953/150‑1 (MQ=255)
                                                                                                  aTGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAatat                                                <  1:3106922/150‑1 (MQ=255)
                                                                                                    ggTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTc                                              <  1:1376956/150‑1 (MQ=255)
                                                                                                        cGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacga                                          >  1:4737977/1‑150 (MQ=255)
                                                                                                         ggATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaa                                         >  2:3813457/1‑150 (MQ=255)
                                                                                                          gATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaac                                        >  1:2203816/1‑150 (MQ=255)
                                                                                                          gATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaac                                        <  2:5145674/150‑1 (MQ=255)
                                                                                                            tctcTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaacga                                      >  2:61329/1‑150 (MQ=255)
                                                                                                            tctcTTTTTTACCCGCGTTGACCATAGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaacga                                      >  2:1472813/1‑150 (MQ=255)
                                                                                                             ctctTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAacgac                                     >  1:2719489/1‑150 (MQ=255)
                                                                                                             ctctTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAacgac                                     <  2:4176879/150‑1 (MQ=255)
                                                                                                             ctctTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAacgac                                     <  2:2215772/150‑1 (MQ=255)
                                                                                                              tctTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAacgacg                                    <  1:2040362/150‑1 (MQ=255)
                                                                                                               ctTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCTCCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACgg                                   >  2:5268533/1‑150 (MQ=255)
                                                                                                               ctTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACgg                                   >  2:1076823/1‑150 (MQ=255)
                                                                                                               ctTTATTACCCGCGTTGACCATTGGCTCGATGCTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCACTATTCACGAACGACgg                                   >  1:2276994/1‑150 (MQ=255)
                                                                                                                ttttttACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGga                                  <  2:1913097/150‑1 (MQ=255)
                                                                                                                     tACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGc                             <  2:3214242/150‑1 (MQ=255)
                                                                                                                     tACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGc                             <  1:3006673/150‑1 (MQ=255)
                                                                                                                         cgcgTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTc                         >  1:1606553/1‑150 (MQ=255)
                                                                                                                         cgcgTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTc                         <  2:4582253/150‑1 (MQ=255)
                                                                                                                          gcgTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCt                        <  2:2232468/150‑1 (MQ=255)
                                                                                                                              tGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCtt                    <  1:1031233/150‑1 (MQ=255)
                                                                                                                              tGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCtt                    >  2:3953493/1‑150 (MQ=255)
                                                                                                                              tGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTAGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCtt                    >  2:2809002/1‑150 (MQ=255)
                                                                                                                               gACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTc                   <  2:4742597/150‑1 (MQ=255)
                                                                                                                                aCCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCg                  >  2:309259/1‑150 (MQ=255)
                                                                                                                                aCCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCg                  >  1:149658/1‑150 (MQ=255)
                                                                                                                                  cATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGAc                >  2:2310735/1‑150 (MQ=255)
                                                                                                                                  cATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGAc                <  2:1375677/150‑1 (MQ=255)
                                                                                                                                   aTTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACa               >  1:537273/1‑150 (MQ=255)
                                                                                                                                     tGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACaaa             <  2:4669155/150‑1 (MQ=255)
                                                                                                                                        cTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAAttt          <  2:2375976/150‑1 (MQ=255)
                                                                                                                                          cGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCt        <  2:4899488/150‑1 (MQ=255)
                                                                                                                                          cGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCt        >  1:1837159/1‑150 (MQ=255)
                                                                                                                                              ggTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTACTGCTTCGACAAATTTATGAAt    >  1:2746286/1‑150 (MQ=255)
                                                                                                                                               gTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATc   <  2:2666307/150‑1 (MQ=255)
                                                                                                                                               gTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATc   <  1:3538790/150‑1 (MQ=255)
                                                                                                                                               gTGATCGTCATCCAAGGTTTCAGTACGACTTTTGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGACGCGACCAATACCGTGTCCGCAATATACACGAAAGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATc   >  1:508576/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGTCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCG  >  CP009273/185246‑185539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGTCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCG  >  CP009273/185246‑185539
                                                                                                                                                   |                                                                                                                                                  
GCGTCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCG                                                                                                                                                  <  SRR19973062.4306604/150‑1 (MQ=60)
        ATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTTCCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCA                                                                                                                                          >  SRR19973062.3674731/1‑150 (MQ=60)
         TTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAG                                                                                                                                         >  SRR19973062.2253706/1‑150 (MQ=60)
         TTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAG                                                                                                                                         >  SRR19973062.2253731/1‑150 (MQ=60)
          TTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTTACCATTTGCTCGATGGTTATCGTCATCCCAGG                                                                                                                                        >  SRR19973062.942894/1‑150 (MQ=60)
          TTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGG                                                                                                                                        <  SRR19973062.803189/150‑1 (MQ=60)
           TCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGT                                                                                                                                       <  SRR19973062.2756465/150‑1 (MQ=60)
            CGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTT                                                                                                                                      >  SRR19973062.5021566/1‑150 (MQ=60)
            CGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCAGTGGCTCGATGGTGATCGTCATCCCAGGTT                                                                                                                                      >  SRR19973062.1787545/1‑150 (MQ=60)
            CGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTATTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATAGTCATCCCAGGTT                                                                                                                                      >  SRR19973062.5021506/1‑150 (MQ=60)
               AGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCA                                                                                                                                   >  SRR19973062.4170635/1‑150 (MQ=60)
               AGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCA                                                                                                                                   >  SRR19973062.4606220/1‑150 (MQ=60)
                 CCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGT                                                                                                                                 <  SRR19973062.4868102/150‑1 (MQ=60)
                  CGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTA                                                                                                                                >  SRR19973062.4360509/1‑150 (MQ=60)
                   GTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTAC                                                                                                                               >  SRR19973062.392560/1‑150 (MQ=60)
                   GTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTAC                                                                                                                               <  SRR19973062.3919684/150‑1 (MQ=60)
                     TATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGA                                                                                                                             >  SRR19973062.367182/1‑150 (MQ=60)
                     TATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGA                                                                                                                             <  SRR19973062.2496764/150‑1 (MQ=60)
                      ATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGAC                                                                                                                            <  SRR19973062.3445865/150‑1 (MQ=60)
                        CAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGT                                                                                                                          >  SRR19973062.2524814/1‑150 (MQ=60)
                         AGTCACCACAAGAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTT                                                                                                                         <  SRR19973062.4155305/150‑1 (MQ=60)
                              CCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTT                                                                                                                    <  SRR19973062.5320639/150‑1 (MQ=60)
                               CACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTC                                                                                                                   >  SRR19973062.2053551/1‑150 (MQ=60)
                                  AATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGTTGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACG                                                                                                                <  SRR19973062.2557892/150‑1 (MQ=60)
                                  AATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACG                                                                                                                <  SRR19973062.2558042/150‑1 (MQ=60)
                                     AGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGA                                                                                                             >  SRR19973062.4613599/1‑150 (MQ=60)
                                     AGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGA                                                                                                             >  SRR19973062.4613591/1‑150 (MQ=60)
                                        ATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTC                                                                                                          >  SRR19973062.240131/1‑150 (MQ=60)
                                          GCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCAT                                                                                                        <  SRR19973062.2492033/150‑1 (MQ=60)
                                          GCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCAT                                                                                                        <  SRR19973062.2138562/150‑1 (MQ=60)
                                           CTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATA                                                                                                       <  SRR19973062.358108/150‑1 (MQ=60)
                                            TCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAG                                                                                                      <  SRR19973062.33971/150‑1 (MQ=60)
                                             CATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGT                                                                                                     <  SRR19973062.5283460/150‑1 (MQ=60)
                                                ATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCA                                                                                                  >  SRR19973062.1666250/1‑150 (MQ=60)
                                                ATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCA                                                                                                  >  SRR19973062.1666390/1‑150 (MQ=60)
                                                   GTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCA                                                                                               <  SRR19973062.1830798/150‑1 (MQ=60)
                                                   GTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCA                                                                                               >  SRR19973062.4207791/1‑150 (MQ=60)
                                                       AGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTG                                                                                           >  SRR19973062.5286393/1‑150 (MQ=60)
                                                        GACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGC                                                                                          <  SRR19973062.3712432/150‑1 (MQ=60)
                                                        GACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGC                                                                                          <  SRR19973062.3350176/150‑1 (MQ=60)
                                                           AAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGT                                                                                       >  SRR19973062.4690412/1‑150 (MQ=60)
                                                              CTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCT                                                                                    <  SRR19973062.311345/150‑1 (MQ=60)
                                                               TGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTT                                                                                   <  SRR19973062.2846770/150‑1 (MQ=60)
                                                                 CGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCA                                                                                 <  SRR19973062.1895861/150‑1 (MQ=60)
                                                                 CGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCA                                                                                 <  SRR19973062.1895860/150‑1 (MQ=60)
                                                                 CGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCA                                                                                 >  SRR19973062.1032237/1‑150 (MQ=60)
                                                                  GATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCAT                                                                                >  SRR19973062.339880/1‑150 (MQ=60)
                                                                  GATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCAT                                                                                <  SRR19973062.2550962/150‑1 (MQ=60)
                                                                  GATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCAT                                                                                >  SRR19973062.2924204/1‑150 (MQ=60)
                                                                   ATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATG                                                                               <  SRR19973062.445337/150‑1 (MQ=60)
                                                                    TCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTAAGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCTTGG                                                                              <  SRR19973062.5200231/150‑1 (MQ=60)
                                                                       TTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAG                                                                           >  SRR19973062.2674613/1‑150 (MQ=60)
                                                                            TTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCG                                                                      >  SRR19973062.2944816/1‑150 (MQ=60)
                                                                             TTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGA                                                                     >  SRR19973062.4067820/1‑150 (MQ=60)
                                                                              TTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACAGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGAC                                                                    <  SRR19973062.4003843/150‑1 (MQ=60)
                                                                              TTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACAGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGAC                                                                    <  SRR19973062.4003825/150‑1 (MQ=60)
                                                                                ACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCA                                                                  >  SRR19973062.4791448/1‑150 (MQ=60)
                                                                                  CGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAAT                                                                >  SRR19973062.217553/1‑150 (MQ=60)
                                                                                  CGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAAT                                                                >  SRR19973062.644315/1‑150 (MQ=60)
                                                                                       AGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGT                                                           <  SRR19973062.511110/150‑1 (MQ=60)
                                                                                       AGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGT                                                           >  SRR19973062.849883/1‑150 (MQ=60)
                                                                                       AGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGT                                                           <  SRR19973062.3979635/150‑1 (MQ=60)
                                                                                       AGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGT                                                           <  SRR19973062.3979432/150‑1 (MQ=60)
                                                                                       AGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGT                                                           >  SRR19973062.5294264/1‑150 (MQ=60)
                                                                                       AGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGT                                                           >  SRR19973062.1580939/1‑150 (MQ=60)
                                                                                         CCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGT                                                         <  SRR19973062.3587867/150‑1 (MQ=60)
                                                                                         CCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGT                                                         >  SRR19973062.437861/1‑150 (MQ=60)
                                                                                         CCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGT                                                         >  SRR19973062.4734170/1‑150 (MQ=60)
                                                                                          CATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGTGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTC                                                        <  SRR19973062.1621658/150‑1 (MQ=60)
                                                                                          CATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTC                                                        <  SRR19973062.1800112/150‑1 (MQ=60)
                                                                                          CATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTC                                                        <  SRR19973062.2383523/150‑1 (MQ=60)
                                                                                          CATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTC                                                        <  SRR19973062.4487704/150‑1 (MQ=60)
                                                                                           ATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCC                                                       <  SRR19973062.2546826/150‑1 (MQ=60)
                                                                                           ATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCC                                                       >  SRR19973062.5346630/1‑150 (MQ=60)
                                                                                           ATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCC                                                       <  SRR19973062.3774912/150‑1 (MQ=60)
                                                                                              TTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCA                                                    >  SRR19973062.3163362/1‑150 (MQ=60)
                                                                                                TCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAAT                                                  <  SRR19973062.1556055/150‑1 (MQ=60)
                                                                                                 CATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATA                                                 >  SRR19973062.2205340/1‑150 (MQ=60)
                                                                                                  ATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCACTACCGTGTCCGCAATAT                                                <  SRR19973062.3708685/150‑1 (MQ=60)
                                                                                                  ATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATAT                                                <  SRR19973062.3122923/150‑1 (MQ=60)
                                                                                                   TGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATT                                               >  SRR19973062.1873695/1‑150 (MQ=60)
                                                                                                    GGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTC                                              <  SRR19973062.1383396/150‑1 (MQ=60)
                                                                                                     GTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCA                                             >  SRR19973062.4663740/1‑150 (MQ=60)
                                                                                                      TGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCAC                                            <  SRR19973062.1978144/150‑1 (MQ=60)
                                                                                                       GCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACG                                           >  SRR19973062.3691223/1‑150 (MQ=60)
                                                                                                       GCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACG                                           <  SRR19973062.85471/150‑1 (MQ=60)
                                                                                                        CGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGA                                          >  SRR19973062.4762674/1‑150 (MQ=60)
                                                                                                        CGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGA                                          >  SRR19973062.5347023/1‑150 (MQ=60)
                                                                                                         GGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAA                                         >  SRR19973062.1591187/1‑150 (MQ=60)
                                                                                                         GGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAA                                         >  SRR19973062.1591140/1‑150 (MQ=60)
                                                                                                          GATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAAC                                        <  SRR19973062.5189555/150‑1 (MQ=60)
                                                                                                          GATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAAC                                        >  SRR19973062.2214786/1‑150 (MQ=60)
                                                                                                          GATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAAC                                        >  SRR19973062.2214869/1‑150 (MQ=60)
                                                                                                            TCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGA                                      >  SRR19973062.4510320/1‑150 (MQ=60)
                                                                                                             CTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGAC                                     >  SRR19973062.2733799/1‑150 (MQ=60)
                                                                                                              TCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACG                                    <  SRR19973062.2050832/150‑1 (MQ=60)
                                                                                                              TCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACG                                    <  SRR19973062.4580628/150‑1 (MQ=60)
                                                                                                               CTTTATTACCCGCGTTGACCATTGGCTCGATGCTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCACTATTCACGAACGACGG                                   >  SRR19973062.2288667/1‑150 (MQ=60)
                                                                                                                     TACCCGCGTTGNCCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAAGACCGTGTCCGCAATATTCACGAACGACGGAGAAGC                             >  SRR19973062.2775600/1‑150 (MQ=60)
                                                                                                                     TACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGC                             <  SRR19973062.3022387/150‑1 (MQ=60)
                                                                                                                      ACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCC                            >  SRR19973062.5204969/1‑150 (MQ=60)
                                                                                                                        CCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTT                          <  SRR19973062.1806841/150‑1 (MQ=60)
                                                                                                                         CGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTC                         >  SRR19973062.1614511/1‑150 (MQ=60)
                                                                                                                         CGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTC                         <  SRR19973062.3586811/150‑1 (MQ=60)
                                                                                                                          GCGTGGACCATGGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATCGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCT                        <  SRR19973062.3905008/150‑1 (MQ=60)
                                                                                                                           CGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTG                       >  SRR19973062.3089313/1‑150 (MQ=60)
                                                                                                                            GTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGC                      >  SRR19973062.1396213/1‑150 (MQ=60)
                                                                                                                              TGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTT                    >  SRR19973062.475362/1‑150 (MQ=60)
                                                                                                                              TGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTT                    <  SRR19973062.1036062/150‑1 (MQ=60)
                                                                                                                                ACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCG                  <  SRR19973062.1246545/150‑1 (MQ=60)
                                                                                                                                ACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCG                  >  SRR19973062.150524/1‑150 (MQ=60)
                                                                                                                                ACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCG                  >  SRR19973062.150429/1‑150 (MQ=60)
                                                                                                                                ACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCG                  <  SRR19973062.4803837/150‑1 (MQ=60)
                                                                                                                                   ATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACA               >  SRR19973062.539807/1‑150 (MQ=60)
                                                                                                                                     TGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAA             <  SRR19973062.4612829/150‑1 (MQ=60)
                                                                                                                                        CTCGATGGTGATCGACATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTT          <  SRR19973062.255536/150‑1 (MQ=60)
                                                                                                                                          CGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCT        >  SRR19973062.1846290/1‑150 (MQ=60)
                                                                                                                                          CGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCT        >  SRR19973062.1846339/1‑150 (MQ=60)
                                                                                                                                           GATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTG       >  SRR19973062.5055171/1‑150 (MQ=60)
                                                                                                                                            ATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGA      >  SRR19973062.4651699/1‑150 (MQ=60)
                                                                                                                                            ATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGA      >  SRR19973062.2216178/1‑150 (MQ=60)
                                                                                                                                             TGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAA     >  SRR19973062.4785143/1‑150 (MQ=60)
                                                                                                                                              GGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAAT    <  SRR19973062.673439/150‑1 (MQ=60)
                                                                                                                                              GGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTACTGCTTCGACAAATTTATGAAT    >  SRR19973062.2760785/1‑150 (MQ=60)
                                                                                                                                               GTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACANATTTCTGAATC   <  SRR19973062.2775452/150‑1 (MQ=60)
                                                                                                                                               GTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATC   <  SRR19973062.1257936/150‑1 (MQ=60)
                                                                                                                                               GTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATC   <  SRR19973062.225687/150‑1 (MQ=60)
                                                                                                                                               GTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATC   <  SRR19973062.3556673/150‑1 (MQ=60)
                                                                                                                                               GTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATC   <  SRR19973062.1553162/150‑1 (MQ=60)
                                                                                                                                               GTGATCGTCATCCAAGGTTTCAGTACGACTTTTGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGACGCGACCAATACCGTGTCCGCAATATACACGAAAGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATC   >  SRR19973062.510957/1‑150 (MQ=60)
                                                                                                                                                TGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCG  <  SRR19973062.103581/150‑1 (MQ=60)
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GCGTCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCG  >  CP009273/185246‑185539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑