Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,129,972 G→T 27.4% D239Y (GAT→TAT)  flgF → flagellar component of cell‑proximal portion of basal‑body rod

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,129,9720GT27.4% 686.9 / 213.3 328D239Y (GAT→TAT) flgFflagellar component of cell‑proximal portion of basal‑body rod
Reads supporting (aligned to +/- strand):  ref base G (134/104);  new base T (41/49);  total (175/153)
Fisher's exact test for biased strand distribution p-value = 8.44e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 346 total aligned reads displayed.

GTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGC  >  CP009273/1129823‑1130121
                                                                                                                                                     |                                                                                                                                                     
  gggCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCgattat                                                                                                                                                     <  1:3520610/150‑1 (MQ=255)
  gggCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCgatgat                                                                                                                                                     <  1:3565155/150‑1 (MQ=255)
  gggCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGGTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGATTTGAAATGCAGATGAAGGTGATCAGCAGCGTCgattat                                                                                                                                                     >  1:4621081/1‑150 (MQ=255)
   ggCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATa                                                                                                                                                    >  2:1329958/1‑150 (MQ=255)
   ggCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATa                                                                                                                                                    >  2:582419/1‑150 (MQ=255)
     ccGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAAc                                                                                                                                                  <  1:4430382/150‑1 (MQ=255)
       ggTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGc                                                                                                                                                >  1:1153177/1‑150 (MQ=255)
        gTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATTATAACGCa                                                                                                                                               >  2:257591/1‑150 (MQ=255)
               aggcagATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATTATAACGCAGGCCGTg                                                                                                                                        <  1:3077729/150‑1 (MQ=255)
                ggcagATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGc                                                                                                                                       >  2:3928649/1‑150 (MQ=255)
                      tCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGGGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGccaacca                                                                                                                                 >  2:334168/1‑150 (MQ=255)
                       ccAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATTATAACGCAGGCCGTGccaaccaa                                                                                                                                >  2:3994280/1‑150 (MQ=255)
                       ccAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGccaaccaa                                                                                                                                >  2:5296639/1‑150 (MQ=255)
                         aaCCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAAct                                                                                                                              <  2:2792947/150‑1 (MQ=255)
                          aCCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAActg                                                                                                                             >  2:20863/1‑150 (MQ=255)
                           ccTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATTATAACGCAGGCCGTGCCAACCAActgc                                                                                                                            <  1:60126/150‑1 (MQ=255)
                            cTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAActgct                                                                                                                           <  2:814327/150‑1 (MQ=255)
                            cTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAAcagct                                                                                                                           >  1:4642334/1‑150 (MQ=255)
                                   gtgATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATg                                                                                                                    <  1:4348019/150‑1 (MQ=255)
                                     gATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATTATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAg                                                                                                                  >  2:3447315/1‑150 (MQ=255)
                                       tGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATTATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGtt                                                                                                                >  1:3254407/1‑150 (MQ=255)
                                           gggggTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAAtt                                                                                                            >  2:3268649/1‑150 (MQ=255)
                                           gggggTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGAGCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAAtt                                                                                                            <  2:267113/150‑1 (MQ=255)
                                            ggggTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTg                                                                                                           >  1:2577009/1‑150 (MQ=255)
                                            ggggTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTg                                                                                                           <  1:4038080/150‑1 (MQ=255)
                                             gggTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGa                                                                                                          >  1:5121756/1‑150 (MQ=255)
                                                ttCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAg                                                                                                       <  1:4853480/150‑1 (MQ=255)
                                                  cTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAAATGCTGTCGATGAGTTAATTGAAAGGa                                                                                                     <  1:960391/150‑1 (MQ=255)
                                                   tGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGAt                                                                                                    >  1:3644230/1‑150 (MQ=255)
                                                    ggAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATa                                                                                                   <  1:3423075/150‑1 (MQ=255)
                                                     gAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATAc                                                                                                  <  1:107546/150‑1 (MQ=255)
                                                      aaGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACa                                                                                                 >  2:3148126/1‑150 (MQ=255)
                                                        ggCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATTATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATg                                                                                               <  2:1555380/150‑1 (MQ=255)
                                                           aGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACa                                                                                            >  2:4684816/1‑150 (MQ=255)
                                                           aGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACa                                                                                            >  2:4431607/1‑150 (MQ=255)
                                                              aaCGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATTATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGt                                                                                         >  2:3093247/1‑150 (MQ=255)
                                                              aaCGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGt                                                                                         >  2:3107948/1‑150 (MQ=255)
                                                              aaCGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGt                                                                                         >  2:626857/1‑150 (MQ=255)
                                                                cGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGtat                                                                                       <  2:4587247/150‑1 (MQ=255)
                                                                cGTCAATGCAGTTGCGGAAATGAGCGACATGATTGCCAGCGCTCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGACAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGtat                                                                                       <  2:3485731/150‑1 (MQ=255)
                                                                 gTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGtata                                                                                      >  1:4989469/1‑150 (MQ=255)
                                                                   cAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAg                                                                                    >  2:1206083/1‑150 (MQ=255)
                                                                    attGCCGTTGCGGAAATTAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTAGATTATAACGCAGGCCGTGCCAACCAACTGCTGTCGATTTGTTAATTGAAAGGATACATGACAAGTATAAGt                                                                                   <  1:1008763/148‑1 (MQ=255)
                                                                       gCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGGGTTAATTGAAAGGATACATTACAAGTATAAGTTGc                                                                                >  1:2314827/1‑150 (MQ=255)
                                                                         cGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGccc                                                                              <  1:2023596/150‑1 (MQ=255)
                                                                            tGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGAt                                                                           <  2:3824609/150‑1 (MQ=255)
                                                                            tGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGACCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGAt                                                                           <  1:375552/150‑1 (MQ=255)
                                                                             gCGGCAATGAGCGACATGAGTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATTATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATg                                                                          <  2:4335272/150‑1 (MQ=255)
                                                                              cGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATTACAAGTATAAGTTGCCCGATgc                                                                         >  1:635838/1‑150 (MQ=255)
                                                                                    tGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGt                                                                   >  2:3654335/1‑150 (MQ=255)
                                                                                     gAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGtt                                                                  >  2:2014656/1‑150 (MQ=255)
                                                                                        cGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTAt                                                               >  2:3532673/1‑150 (MQ=255)
                                                                                        cGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTAt                                                               <  1:221374/150‑1 (MQ=255)
                                                                                         gACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATTATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATc                                                              <  1:4130847/150‑1 (MQ=255)
                                                                                         gACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATTATAACGCACGCCGTGCCAACCAACAGCAGTCGATGAGTTAATTGAAAGGATAAAAGACAAGTATAAGTTGCCCGCTGCGCAAGTTTATc                                                              >  1:3143275/1‑150 (MQ=255)
                                                                                         gACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATc                                                              >  1:840452/1‑150 (MQ=255)
                                                                                         gACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATc                                                              <  1:1691526/150‑1 (MQ=255)
                                                                                            aTGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATTATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCggg                                                           >  1:2875956/1‑150 (MQ=255)
                                                                                             tGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGAACAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGt                                                          <  1:2788668/150‑1 (MQ=255)
                                                                                               aTTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCt                                                        <  2:2334474/150‑1 (MQ=255)
                                                                                                  gCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATg                                                     <  1:1330074/150‑1 (MQ=255)
                                                                                                   ccAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATgg                                                    <  2:1123700/150‑1 (MQ=255)
                                                                                                   ccAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATgg                                                    <  2:5127666/150‑1 (MQ=255)
                                                                                                   ccAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATgg                                                    <  2:4096633/150‑1 (MQ=255)
                                                                                                    aaGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAAAGCAGGCCGTGCCAACCAAATTCAGTAGATGAGATAATTTAAAGGATACATTACAAGTATAAGTAGCCCGATGCGCAAGATTATCGGGTCTATggg                                                   >  2:145119/2‑150 (MQ=255)
                                                                                                     aGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATTATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATgggg                                                  <  2:2473621/150‑1 (MQ=255)
                                                                                                     aGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATgggg                                                  >  2:226687/1‑150 (MQ=255)
                                                                                                       cgcgcgGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATAGGGTCTATGGGGGc                                                >  1:179464/1‑150 (MQ=255)
                                                                                                        gcgcgGCGTTTTGAAATGCAGATGACGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCa                                               <  2:1737934/150‑1 (MQ=255)
                                                                                                        gcgcgGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGAAAGTTTATAGGGTCTATGGGGGCa                                               >  2:5067204/1‑150 (MQ=255)
                                                                                                           cggcgTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATc                                            <  1:2262730/150‑1 (MQ=255)
                                                                                                             gcgTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGc                                          >  2:1840801/1‑150 (MQ=255)
                                                                                                               gTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATTATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCaa                                        >  2:49596/1‑150 (MQ=255)
                                                                                                               gTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATTATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCaa                                        >  2:1029960/1‑150 (MQ=255)
                                                                                                                ttttGAAATGCAGATGAAGGTGACCAGCAGCGTCGATTATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACCTGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAAt                                       <  1:858571/150‑1 (MQ=255)
                                                                                                                 tttGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAAtt                                      <  1:4165231/150‑1 (MQ=255)
                                                                                                                    gAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTAt                                   <  2:2841211/150‑1 (MQ=255)
                                                                                                                       aTGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGa                                >  1:1261796/1‑150 (MQ=255)
                                                                                                                           aGATGAAGGTGATCAGCAGCGTCGATTATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGAtttt                            <  2:1048525/150‑1 (MQ=255)
                                                                                                                           aGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGAtttt                            >  1:3689071/1‑150 (MQ=255)
                                                                                                                           aGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGAtttt                            >  2:1299253/1‑150 (MQ=255)
                                                                                                                             aTGAAGGTGATCAGCAGCGTCGATTATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGc                          >  2:4730886/1‑150 (MQ=255)
                                                                                                                             aTGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGc                          >  1:3866190/1‑150 (MQ=255)
                                                                                                                              tGAAGGTGATCAGCAGCGTCGATTATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCg                         <  1:3994280/150‑1 (MQ=255)
                                                                                                                                   gTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCAc                    >  2:4140863/1‑150 (MQ=255)
                                                                                                                                   gTGATCAGCAGCGACGATGAAAACGCAGGCCGTGCCAACCAACAGCAGTCGTTGATTTAATAGAAAGGAGACATGAAAAGTAGAAGAAGCCCGATGCGCAAGTTTATCGGGTCAATGGGGGCAAACGCAAATTATCCATTTAGCGAGCAc                    >  2:4907782/1‑150 (MQ=255)
                                                                                                                                    tGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACt                   <  1:1048066/150‑1 (MQ=255)
                                                                                                                                    tGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACt                   <  1:3937985/150‑1 (MQ=255)
                                                                                                                                    tGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACt                   >  2:4470719/1‑150 (MQ=255)
                                                                                                                                     gATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACtt                  >  2:4762315/1‑150 (MQ=255)
                                                                                                                                         agcagcGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAg              <  1:4562079/150‑1 (MQ=255)
                                                                                                                                            agcGTCGATTATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGcc           <  1:138225/150‑1 (MQ=255)
                                                                                                                                              cGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCgg         >  2:2304450/1‑150 (MQ=255)
                                                                                                                                               gTCGATTATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGa        <  1:3509424/150‑1 (MQ=255)
                                                                                                                                               gTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGa        >  2:2991927/1‑150 (MQ=255)
                                                                                                                                                 cGATTATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATa      >  1:264904/1‑150 (MQ=255)
                                                                                                                                                 cGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATa      >  1:1732052/1‑150 (MQ=255)
                                                                                                                                                  gattatACCGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATaa     <  2:4984246/150‑1 (MQ=255)
                                                                                                                                                    tgatAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAgg   >  1:2199380/1‑150 (MQ=255)
                                                                                                                                                     gatAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGc  >  1:5192958/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGC  >  CP009273/1129823‑1130121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A