Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,542,503 G→T 32.5% T282T (ACG→ACT)  fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,542,5030GT32.5% 585.9 / 275.1 347T282T (ACG→ACT) fdnGformate dehydrogenase‑N, alpha subunit, nitrate‑inducible
Reads supporting (aligned to +/- strand):  ref base G (125/109);  new base T (61/52);  total (186/161)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 351 total aligned reads displayed.

GCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCA  >  CP009273/1542354‑1542652
                                                                                                                                                     |                                                                                                                                                     
  tgctgAAGCGCATCCCGTCGGTTTCCGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACttt                                                                                                                                                     >  2:3150311/1‑150 (MQ=255)
  tgctgAAGCGCATCCCGTCGGTTTCCGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGtt                                                                                                                                                     >  1:4817867/1‑150 (MQ=255)
  tgctgAAGCGCATCCCGTCGGTTTCCGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTGTTACGCGGACCGCTTCGGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACCTTACGtt                                                                                                                                                     >  1:2897219/1‑150 (MQ=255)
   gctgAAGCGCATCCCGTCGGTTTCCGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTc                                                                                                                                                    <  1:1987002/150‑1 (MQ=255)
     tgAAGCGCATCCCGTCGGTTTCCGCGGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCt                                                                                                                                                  <  2:3395491/150‑1 (MQ=255)
         gcgcATCCCGTCGGTTTCCGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCt                                                                                                                                              <  2:3171698/150‑1 (MQ=255)
          cgcATCCCGTCGGTTTCCGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTg                                                                                                                                             <  1:3027918/150‑1 (MQ=255)
           gcNTCCCGTCGGTTTCCGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTgg                                                                                                                                            >  1:383291/1‑150 (MQ=255)
           gcATCCCGTCGGTTTCCGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTgg                                                                                                                                            <  2:1688323/150‑1 (MQ=255)
             aTCCCGTCGGTTTCCGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCg                                                                                                                                          >  2:4129972/1‑150 (MQ=255)
               cccGTCGGTTTCCGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGtt                                                                                                                                        >  2:1319861/1‑150 (MQ=255)
                      gTTTCCGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCt                                                                                                                                 <  2:1118195/150‑1 (MQ=255)
                       tttCCGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTa                                                                                                                                <  1:426625/150‑1 (MQ=255)
                       tttCCGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTa                                                                                                                                <  1:2388134/150‑1 (MQ=255)
                        ttCCGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTAc                                                                                                                               >  1:2128899/1‑150 (MQ=255)
                          ccGCTGGGCGATGGAAGCGAAAAACACCAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCt                                                                                                                             <  2:1428962/150‑1 (MQ=255)
                          ccGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCt                                                                                                                             >  1:4233729/1‑150 (MQ=255)
                             cTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTAGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGAt                                                                                                                          >  1:1370533/1‑150 (MQ=255)
                              tGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATc                                                                                                                         >  1:1092213/1‑150 (MQ=255)
                              tGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATc                                                                                                                         <  2:638541/150‑1 (MQ=255)
                               gggCTATGGAAGCTAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCg                                                                                                                        <  1:515409/150‑1 (MQ=255)
                               gggCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCg                                                                                                                        <  2:3995610/150‑1 (MQ=255)
                               gggCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCg                                                                                                                        <  2:4072389/150‑1 (MQ=255)
                               gggCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCg                                                                                                                        >  2:2641694/1‑150 (MQ=255)
                                   gATGGAAGCGAAAAACAACCACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGataa                                                                                                                    <  2:2675735/150‑1 (MQ=255)
                                   gATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGaaaa                                                                                                                    <  2:5127368/150‑1 (MQ=255)
                                      ggAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTGTTGCGCTACCTGATCGAAaacaa                                                                                                                 >  2:2090792/1‑150 (MQ=255)
                                       gAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAaacaac                                                                                                                <  1:4658462/150‑1 (MQ=255)
                                          gCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACaaa                                                                                                             <  2:5175928/150‑1 (MQ=255)
                                           cGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACaaaa                                                                                                            <  1:327812/150‑1 (MQ=255)
                                            gAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAAt                                                                                                           >  1:4301558/1‑150 (MQ=255)
                                             aaaaaCAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATc                                                                                                          <  2:4213659/150‑1 (MQ=255)
                                               aaaCAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCaa                                                                                                        >  1:5146709/1‑150 (MQ=255)
                                                  caacaacGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGc                                                                                                     >  1:4967304/1‑150 (MQ=255)
                                                   aacaacGACGCNACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGcc                                                                                                    >  1:2764642/1‑150 (MQ=255)
                                                    acaacGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCTTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCg                                                                                                   <  1:3153957/150‑1 (MQ=255)
                                                     caacGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGa                                                                                                  >  2:4562574/1‑150 (MQ=255)
                                                      aacGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGaa                                                                                                 >  2:4736231/1‑150 (MQ=255)
                                                      aacGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGaa                                                                                                 >  2:74320/1‑150 (MQ=255)
                                                             cAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTa                                                                                          >  2:802016/1‑150 (MQ=255)
                                                             cAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTa                                                                                          <  1:10407/150‑1 (MQ=255)
                                                             cAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTa                                                                                          >  1:2672718/1‑150 (MQ=255)
                                                             cAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAACACGTTa                                                                                          <  2:2394862/150‑1 (MQ=255)
                                                               aCCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAg                                                                                        <  1:3752465/150‑1 (MQ=255)
                                                                ccTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGc                                                                                       >  1:3011877/1‑150 (MQ=255)
                                                                ccTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGc                                                                                       >  1:4476599/1‑150 (MQ=255)
                                                                 cTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCa                                                                                      >  1:1874219/1‑150 (MQ=255)
                                                                  ttGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCAt                                                                                     <  2:4470395/150‑1 (MQ=255)
                                                                  ttGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCAt                                                                                     <  1:4186791/150‑1 (MQ=255)
                                                                  ttGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCAt                                                                                     >  2:1484127/1‑150 (MQ=255)
                                                                   tGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCAtt                                                                                    <  2:1937780/150‑1 (MQ=255)
                                                                     aTCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTac                                                                                  >  1:2338024/1‑150 (MQ=255)
                                                                       cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTacac                                                                                >  1:5062895/1‑150 (MQ=255)
                                                                       cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTacac                                                                                >  2:575720/1‑150 (MQ=255)
                                                                        gTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCAGTACAcc                                                                               >  2:2957539/1‑150 (MQ=255)
                                                                         ttGTCGATCCCCGATTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCa                                                                              >  2:1805022/1‑150 (MQ=255)
                                                                          tGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCaa                                                                             >  1:1509372/1‑150 (MQ=255)
                                                                           gTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAAc                                                                            <  2:1748466/150‑1 (MQ=255)
                                                                           gTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACACTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAAc                                                                            >  1:4581537/1‑150 (MQ=255)
                                                                            tCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTCCCTGATCGAAAACACCAAAATCAACGCCGAATACGTTAAGCATTACACCCACg                                                                           <  2:3564220/150‑1 (MQ=255)
                                                                            tCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACg                                                                           <  1:5186882/150‑1 (MQ=255)
                                                                            tCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACg                                                                           >  1:2284381/1‑150 (MQ=255)
                                                                              gATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGcc                                                                         >  2:875178/1‑150 (MQ=255)
                                                                              gATCCCCGTTTTACGCGTACCACTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGcc                                                                         >  1:948812/1‑150 (MQ=255)
                                                                              gATCCCCGGTGTACGCGTCCCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGcc                                                                         <  1:256676/150‑1 (MQ=255)
                                                                                tCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAg                                                                       >  1:3430022/1‑150 (MQ=255)
                                                                                   ccGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCct                                                                    <  1:4414030/150‑1 (MQ=255)
                                                                                    cGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCctg                                                                   <  1:3049019/150‑1 (MQ=255)
                                                                                      ttttACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCctgct                                                                 <  2:4839347/150‑1 (MQ=255)
                                                                                         tACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGt                                                              >  1:1402740/1‑150 (MQ=255)
                                                                                         tACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGt                                                              <  1:658161/150‑1 (MQ=255)
                                                                                                aCCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGtgat                                                       >  2:4438423/1‑150 (MQ=255)
                                                                                                         gtgGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCt                                              >  1:4555984/1‑150 (MQ=255)
                                                                                                           ggCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCttt                                            >  2:4928428/1‑150 (MQ=255)
                                                                                                           ggCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCttt                                            <  1:2504043/150‑1 (MQ=255)
                                                                                                             cGGATATTTCCGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCg                                          <  2:3702730/150‑1 (MQ=255)
                                                                                                             cGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCg                                          <  2:1254372/150‑1 (MQ=255)
                                                                                                             cGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCg                                          >  1:2234963/1‑150 (MQ=255)
                                                                                                              ggATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGa                                         >  1:101865/1‑150 (MQ=255)
                                                                                                                atatTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAg                                       >  2:2553229/1‑150 (MQ=255)
                                                                                                                atatTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAg                                       >  2:1517404/1‑150 (MQ=255)
                                                                                                                 tatTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGa                                      <  2:3497671/150‑1 (MQ=255)
                                                                                                                  atTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGAc                                     <  2:4609893/150‑1 (MQ=255)
                                                                                                                       cgcgCCTATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCt                                <  2:3905398/150‑1 (MQ=255)
                                                                                                                       cgcgCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCt                                <  2:2207760/150‑1 (MQ=255)
                                                                                                                          gcCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGtt                             >  2:5240560/1‑150 (MQ=255)
                                                                                                                           ccTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTc                            >  2:3322562/1‑150 (MQ=255)
                                                                                                                            cTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCa                           <  1:1170269/150‑1 (MQ=255)
                                                                                                                             tATTCGTTCCGGTACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAg                          <  2:3841061/150‑1 (MQ=255)
                                                                                                                                        gTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTacgacg               >  2:2879981/1‑150 (MQ=255)
                                                                                                                                         tACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGc              >  2:327400/1‑150 (MQ=255)
                                                                                                                                         tACGGACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGc              >  1:3606401/1‑150 (MQ=255)
                                                                                                                                          aCGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCt             <  1:5289463/150‑1 (MQ=255)
                                                                                                                                          aCGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCt             <  2:5288504/150‑1 (MQ=255)
                                                                                                                                            ggACATTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCCGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGa           >  2:2016528/1‑150 (MQ=255)
                                                                                                                                               cATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGaaaa        >  2:2464186/1‑150 (MQ=255)
                                                                                                                                                aTTACTTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGaaaaa       >  1:1578019/1‑150 (MQ=255)
                                                                                                                                                aTTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAAGAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGaaaaa       <  2:3016568/150‑1 (MQ=255)
                                                                                                                                                aTTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGaaaaa       <  1:1689850/150‑1 (MQ=255)
                                                                                                                                                    cGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTc   <  2:4758340/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCA  >  CP009273/1542354‑1542652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A