Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,557,390 G→T 11.0% intergenic (‑57/+201) ddpX ← / ← dosP D‑ala‑D‑ala dipeptidase, Zn‑dependent/oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,557,3900GT11.0% 1177.2 / 73.8 352intergenic (‑57/+201)ddpX/dosPD‑ala‑D‑ala dipeptidase, Zn‑dependent/oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator
Reads supporting (aligned to +/- strand):  ref base G (156/157);  new base T (12/27);  total (168/184)
Fisher's exact test for biased strand distribution p-value = 2.74e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 382 total aligned reads displayed.

ACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAG  >  CP009273/1557241‑1557539
                                                                                                                                                     |                                                                                                                                                     
aCCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatg                                                                                                                                                       >  1:4888916/1‑150 (MQ=255)
   tgtgATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatgcca                                                                                                                                                    <  1:2619308/150‑1 (MQ=255)
    gtgATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatgccat                                                                                                                                                   >  1:3129735/1‑150 (MQ=255)
    gtgATGTTATCAGCGCAGGCGCATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatgccat                                                                                                                                                   <  2:4645674/150‑1 (MQ=255)
    gtgATGTTATAAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatgccat                                                                                                                                                   >  2:403457/1‑150 (MQ=255)
     tgATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatgccatg                                                                                                                                                  >  1:33497/1‑150 (MQ=255)
         gTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCgg                                                                                                                                              >  2:3482847/1‑150 (MQ=255)
            aTCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGAt                                                                                                                                           >  1:4480899/1‑150 (MQ=255)
            aTCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGAt                                                                                                                                           <  1:3497705/150‑1 (MQ=255)
             tCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATTCCATGCCGGGATa                                                                                                                                          <  2:4127265/150‑1 (MQ=255)
              cAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATaa                                                                                                                                         >  2:1760991/1‑150 (MQ=255)
               aGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTATGGCTAAATCAACCAGTTCGGTGGTATACGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCAACACGGGATAAg                                                                                                                                        >  1:55932/1‑150 (MQ=255)
               aGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATTCCATGCCGGGATAAg                                                                                                                                        <  2:3666092/150‑1 (MQ=255)
               aGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAg                                                                                                                                        >  2:19297/1‑150 (MQ=255)
               aGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAg                                                                                                                                        >  1:413079/1‑150 (MQ=255)
                gcgcAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAgc                                                                                                                                       <  1:1500050/150‑1 (MQ=255)
                    aGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGGTTACGGCTAAATCAACCAGTTCGGTGGTTTCCGACATATACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGACATGCCGGGATAAGCGCCa                                                                                                                                   <  2:4960135/150‑1 (MQ=255)
                    aGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCa                                                                                                                                   >  1:2814878/1‑150 (MQ=255)
                    aGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCa                                                                                                                                   >  1:5151815/1‑150 (MQ=255)
                        gTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATTCCATGCCGGGATAAGCGCCAGAAt                                                                                                                               <  2:2781557/150‑1 (MQ=255)
                         tATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGGTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATg                                                                                                                              <  2:630927/150‑1 (MQ=255)
                           tttCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCt                                                                                                                            <  1:2419655/150‑1 (MQ=255)
                            ttCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTg                                                                                                                           >  2:1326230/1‑150 (MQ=255)
                             tCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTgg                                                                                                                          <  1:4241953/150‑1 (MQ=255)
                               aaTTCGATCTCCCGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCt                                                                                                                        >  2:203451/1‑150 (MQ=255)
                                aTTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCtt                                                                                                                       >  2:1522011/1‑150 (MQ=255)
                                 ttCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATTCCATGCCGGGATAAGCGCCAGAATGCTGGCTTa                                                                                                                      <  1:5069998/150‑1 (MQ=255)
                                 ttCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTa                                                                                                                      <  1:1071137/150‑1 (MQ=255)
                                  tCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTaa                                                                                                                     >  2:4516002/1‑150 (MQ=255)
                                   cGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTaaa                                                                                                                    >  1:3878294/1‑150 (MQ=255)
                                      tctcCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATTCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGtt                                                                                                                 <  1:3411441/150‑1 (MQ=255)
                                      tctcCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGtt                                                                                                                 <  1:3702875/150‑1 (MQ=255)
                                        tcCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTtat                                                                                                               <  1:1036693/150‑1 (MQ=255)
                                         ccAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTtata                                                                                                              >  2:2348794/1‑150 (MQ=255)
                                         ccAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTtata                                                                                                              >  1:4364709/1‑150 (MQ=255)
                                           aGATCAGGTAAGATTACGGCTAAATCAACCAGTTCGGTGGTATACGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTTTGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATAtt                                                                                                            <  1:5243607/150‑1 (MQ=255)
                                           aGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATAtt                                                                                                            <  1:397306/150‑1 (MQ=255)
                                           aGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATAtt                                                                                                            <  2:1641855/150‑1 (MQ=255)
                                            gATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATAttt                                                                                                           >  2:4718714/1‑150 (MQ=255)
                                                 gggAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATTCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTaa                                                                                                      <  2:929565/150‑1 (MQ=255)
                                                     aGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACttt                                                                                                  <  2:486721/150‑1 (MQ=255)
                                                      gATTACGGCTAAATCAACCAGTTCGGTGGTTTCAGACATAAAGGGTTCCTGTATAAGACAAATATTTCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACtttt                                                                                                 <  1:1990270/150‑1 (MQ=255)
                                                         tACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGAt                                                                                              <  2:3962979/150‑1 (MQ=255)
                                                           cGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATTCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCa                                                                                            <  1:225415/150‑1 (MQ=255)
                                                            ggCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCaa                                                                                           <  1:4010695/150‑1 (MQ=255)
                                                              cTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACa                                                                                         >  2:2521391/1‑150 (MQ=255)
                                                              cTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACa                                                                                         <  2:36233/150‑1 (MQ=255)
                                                               tAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACAt                                                                                        <  1:3149760/150‑1 (MQ=255)
                                                                 aaTCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATTCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACAttt                                                                                      <  1:2533227/150‑1 (MQ=255)
                                                                 aaTCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATTCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACAttt                                                                                      <  1:751845/150‑1 (MQ=255)
                                                                   tCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATTCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTtgt                                                                                    >  1:3230698/1‑150 (MQ=255)
                                                                   tCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATTCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTtgt                                                                                    >  1:1187334/1‑150 (MQ=255)
                                                                         aGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCg                                                                              >  2:1289727/1‑150 (MQ=255)
                                                                         aGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCg                                                                              <  1:4753319/150‑1 (MQ=255)
                                                                           ttCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTa                                                                            >  2:5305301/1‑150 (MQ=255)
                                                                                   tATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGtt                                                                    >  1:4976101/1‑150 (MQ=255)
                                                                                   tATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGtt                                                                    >  1:134503/1‑150 (MQ=255)
                                                                                    aTCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGttt                                                                   <  2:3073902/150‑1 (MQ=255)
                                                                                     tCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGtttt                                                                  <  2:354786/150‑1 (MQ=255)
                                                                                     tCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGtttt                                                                  >  2:216202/1‑150 (MQ=255)
                                                                                      ccGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTg                                                                 >  2:5160211/1‑150 (MQ=255)
                                                                                        gACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGt                                                               >  2:265735/1‑150 (MQ=255)
                                                                                         aCATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTg                                                              >  2:714398/1‑150 (MQ=255)
                                                                                                 ggTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAggg                                                      <  1:2590126/150‑1 (MQ=255)
                                                                                                 ggTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAggg                                                      >  1:4417425/1‑150 (MQ=255)
                                                                                                  gTTCCTGTATAAGACAGAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAgggg                                                     <  2:606832/150‑1 (MQ=255)
                                                                                                  gTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAgggg                                                     >  1:2183023/1‑150 (MQ=255)
                                                                                                  gTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAgggg                                                     >  1:2078714/1‑150 (MQ=255)
                                                                                                   ttCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGa                                                    <  2:2660342/150‑1 (MQ=255)
                                                                                                        gTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTa                                               >  2:1849872/1‑150 (MQ=255)
                                                                                                        gTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTa                                               >  1:2971641/1‑150 (MQ=255)
                                                                                                         tataAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTaa                                              >  1:105406/1‑150 (MQ=255)
                                                                                                           taAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTaagg                                            >  2:4405974/1‑150 (MQ=255)
                                                                                                              gACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTaaggaag                                         <  2:2265237/150‑1 (MQ=255)
                                                                                                               aCAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTaaggaagg                                        <  2:5272390/150‑1 (MQ=255)
                                                                                                                  aaaaTTGCTGCGCTTTCCGCTTATGCAGATCTCATTCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAAt                                     >  1:601338/1‑150 (MQ=255)
                                                                                                                  aaaaTTGCTGCGCTTTCCGCTTATGCAGATCTCATTCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAAt                                     >  1:3278414/1‑150 (MQ=255)
                                                                                                                      ttGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTcc                                 <  1:3923338/150‑1 (MQ=255)
                                                                                                                       tgctgcGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTTATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGATGGGAAGTTAAGTAAGGAAtctcac                                >  1:1864964/1‑148 (MQ=255)
                                                                                                                            cgcTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAAt                           >  2:2458811/1‑150 (MQ=255)
                                                                                                                            cgcTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCACCCGGAAt                           <  1:1641869/150‑1 (MQ=255)
                                                                                                                              cTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGACGTTAAGGAAGGAATCTCCCGGAATCg                         >  2:1377176/1‑150 (MQ=255)
                                                                                                                               tttCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGt                        >  2:2908631/1‑150 (MQ=255)
                                                                                                                               tttCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGt                        <  2:1066302/150‑1 (MQ=255)
                                                                                                                                ttCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTa                       >  1:301196/1‑150 (MQ=255)
                                                                                                                                ttCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTa                       <  1:2755758/150‑1 (MQ=255)
                                                                                                                                    gCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGACTCGTAGCTg                   >  1:2861802/1‑150 (MQ=255)
                                                                                                                                    gCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTg                   <  1:1002354/150‑1 (MQ=255)
                                                                                                                                        aTGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAAt               >  2:2391149/1‑150 (MQ=255)
                                                                                                                                           cAGATCTCATTCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATcac            <  1:4203353/150‑1 (MQ=255)
                                                                                                                                            aGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATGTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATcaca           >  1:3640802/1‑150 (MQ=255)
                                                                                                                                                 tcATTCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTAtt      <  2:2248915/150‑1 (MQ=255)
                                                                                                                                                 tcATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTAtt      <  2:3155642/150‑1 (MQ=255)
                                                                                                                                                 tcATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTAtt      <  1:4565949/150‑1 (MQ=255)
                                                                                                                                                 tcATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTGTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAAGCGTAGCTGAAATCACAGTAtt      >  2:5048951/1‑150 (MQ=255)
                                                                                                                                                  catgccatgcCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTAttt     >  2:1463986/1‑150 (MQ=255)
                                                                                                                                                   atgccatgccGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTa    <  2:3272874/150‑1 (MQ=255)
                                                                                                                                                     gccatgccGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAg  >  1:834431/1‑150 (MQ=255)
                                                                                                                                                     gccatgccGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAg  <  1:2307150/150‑1 (MQ=255)
                                                                                                                                                     gccatgccGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAg  >  1:4803989/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAG  >  CP009273/1557241‑1557539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A