Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,899,504 T→C 13.1% V149V (GTT→GTC)  yobD → UPF0266 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,899,5040TC13.1% 988.7 / 121.9 367V149V (GTT→GTC) yobDUPF0266 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (139/180);  new base C (22/26);  total (161/206)
Fisher's exact test for biased strand distribution p-value = 8.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 380 total aligned reads displayed.

TTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAATCGTTATCAA  >  CP009273/1899355‑1899653
                                                                                                                                                     |                                                                                                                                                     
ttcttcGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGtt                                                                                                                                                       >  1:4896826/1‑150 (MQ=255)
ttcttcGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGtt                                                                                                                                                       >  1:603057/1‑150 (MQ=255)
  cttcGCCAATGTCTGTATTTAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTc                                                                                                                                                     <  1:4676858/150‑1 (MQ=255)
  cttcGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTc                                                                                                                                                     >  2:2817508/1‑150 (MQ=255)
     cGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAAc                                                                                                                                                  >  2:3728105/1‑150 (MQ=255)
         aaTGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCaa                                                                                                                                              >  1:4087/1‑150 (MQ=255)
          aTGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAAt                                                                                                                                             >  2:1153103/1‑150 (MQ=255)
           tGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATa                                                                                                                                            >  1:4731855/1‑150 (MQ=255)
           tGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATa                                                                                                                                            >  2:4447384/1‑150 (MQ=255)
            gTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATaa                                                                                                                                           >  1:4022514/1‑150 (MQ=255)
            gTCTGGATTGAATATAGCCGAATAAAATCGATGAACTTTTCGGAAGATGGCGTGTTGTTGATGCAATTAGAACAGCGTCTTCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCTTTTCAACTCAATaa                                                                                                                                           >  1:3801161/1‑150 (MQ=255)
             tCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAg                                                                                                                                          <  2:101294/150‑1 (MQ=255)
              cTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGt                                                                                                                                         <  2:1625458/150‑1 (MQ=255)
              cTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTCTCAACTCAATAAGt                                                                                                                                         >  2:4128293/1‑150 (MQ=255)
                 gATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTAt                                                                                                                                      >  2:4282444/1‑150 (MQ=255)
                 gATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTCTCAACTCAATAAGTTAt                                                                                                                                      >  1:2974064/1‑150 (MQ=255)
                  aTTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATg                                                                                                                                     >  1:2644106/1‑150 (MQ=255)
                     gAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAAt                                                                                                                                  >  1:864000/1‑150 (MQ=255)
                         ataGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTCTCAACTCAATAAGTTATGAATTTAg                                                                                                                              <  2:5328696/150‑1 (MQ=255)
                             ccGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCaa                                                                                                                          <  1:1705941/150‑1 (MQ=255)
                                aaTCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGc                                                                                                                       >  1:5094997/1‑150 (MQ=255)
                                aaTCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGc                                                                                                                       <  1:3510166/150‑1 (MQ=255)
                                  tCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTCTCAACTCAATAAGTTATGAATTTAGCCAAAGCtc                                                                                                                     >  1:2773524/1‑149 (MQ=255)
                                   cAAAGCGATGACCTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTAt                                                                                                                    <  2:3902353/150‑1 (MQ=255)
                                   cAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTCTCAACTCAATAAGTTATGAATTTAGCCAAAGCTAt                                                                                                                    <  2:4748557/150‑1 (MQ=255)
                                    aaaGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATg                                                                                                                   >  1:2449706/1‑150 (MQ=255)
                                    aaaGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATg                                                                                                                   >  1:3166732/1‑150 (MQ=255)
                                       gCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGttt                                                                                                                <  2:2736810/150‑1 (MQ=255)
                                        cGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTCTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTa                                                                                                               >  1:3630269/1‑150 (MQ=255)
                                         gATGAACTTGTAGGAAGATGGAGTGTTGGTGATGCAATTAGAAAAGCGTCGGCTGTTAATCAGCGTTAGAAATATCGAAGATCTGGAAAAAATTTATAATCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGTCAAAGATATGTTTat                                                                                                              <  2:2977275/150‑2 (MQ=255)
                                          aTGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAgt                                                                                                             <  2:3885515/150‑1 (MQ=255)
                                           tGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAgtg                                                                                                            <  1:4268825/150‑1 (MQ=255)
                                            gAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAgtgt                                                                                                           >  2:1193496/1‑150 (MQ=255)
                                            gAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAgtgt                                                                                                           >  2:1907416/1‑150 (MQ=255)
                                             aaCTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTCTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTa                                                                                                          >  2:3441326/1‑150 (MQ=255)
                                                  gTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTAttttt                                                                                                     <  1:1203852/150‑1 (MQ=255)
                                                      gAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTtaata                                                                                                 >  2:4260843/1‑150 (MQ=255)
                                                        aGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTaataat                                                                                               >  2:4033900/1‑150 (MQ=255)
                                                        aGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTaataat                                                                                               <  2:1106080/150‑1 (MQ=255)
                                                        aGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTaataat                                                                                               <  2:4390996/150‑1 (MQ=255)
                                                        aGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTaataat                                                                                               >  1:1280859/1‑150 (MQ=255)
                                                               gtgtTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTAAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTATTAATCAGAAAt                                                                                        >  1:1440408/1‑150 (MQ=255)
                                                               gtgtTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTCTCAACTCAATAAGTTATGAATTTAGCCAAAGCGATGGTTAGTGTATTTTTAATAATCAGACAt                                                                                        >  1:779938/1‑150 (MQ=255)
                                                                tgtTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATa                                                                                       <  1:4978644/150‑1 (MQ=255)
                                                                 gtTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAg                                                                                      <  2:3504012/150‑1 (MQ=255)
                                                                 gtTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAg                                                                                      <  1:5244158/150‑1 (MQ=255)
                                                                 gtTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAg                                                                                      <  2:697076/150‑1 (MQ=255)
                                                                   tGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTCTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCt                                                                                    <  1:47992/150‑1 (MQ=255)
                                                                    ggTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTCTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCtt                                                                                   <  2:3947938/150‑1 (MQ=255)
                                                                     gTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGATTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTa                                                                                  <  1:1039221/150‑1 (MQ=255)
                                                                      tGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAg                                                                                 <  1:1027651/150‑1 (MQ=255)
                                                                         tGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCGATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCt                                                                              >  2:1966933/1‑150 (MQ=255)
                                                                          gCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCta                                                                             <  1:3089513/150‑1 (MQ=255)
                                                                           cAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCtat                                                                            >  1:4202201/1‑150 (MQ=255)
                                                                            aaTTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCtata                                                                           <  2:2060798/150‑1 (MQ=255)
                                                                               tAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTa                                                                        <  1:901408/150‑1 (MQ=255)
                                                                                 gAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTAcc                                                                      >  2:4853324/1‑150 (MQ=255)
                                                                                   aCAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACctc                                                                    <  2:4689792/150‑1 (MQ=255)
                                                                                     aGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCtt                                                                  >  1:1230209/1‑150 (MQ=255)
                                                                                        gTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTccc                                                               <  2:2367922/150‑1 (MQ=255)
                                                                                         tCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCt                                                              >  2:2556777/1‑150 (MQ=255)
                                                                                             cTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTAtt                                                          <  1:1806/150‑1 (MQ=255)
                                                                                             cTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTAtt                                                          <  2:2435447/150‑1 (MQ=255)
                                                                                             cTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTAtt                                                          >  1:4145854/1‑150 (MQ=255)
                                                                                             cTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTAtt                                                          <  1:226987/150‑1 (MQ=255)
                                                                                              tGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTAttt                                                         <  2:3160777/150‑1 (MQ=255)
                                                                                               gTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTg                                                        >  2:1314339/1‑150 (MQ=255)
                                                                                                     ccGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGttattt                                                  <  1:1631136/150‑1 (MQ=255)
                                                                                                      cgcgTTCGAAATATCGACGATCTGGAAAAAATTCATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGttattta                                                 >  1:4309474/1‑150 (MQ=255)
                                                                                                        cgTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTtatttatt                                               >  1:4501775/1‑150 (MQ=255)
                                                                                                           tCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTa                                            <  1:1944848/150‑1 (MQ=255)
                                                                                                             gAAATATCGACGATCTGGTAATATTTTATAAGCGTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAAc                                          <  1:3075240/150‑1 (MQ=255)
                                                                                                             gAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAAc                                          <  1:4755085/150‑1 (MQ=255)
                                                                                                              aaaTATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACg                                         <  2:1494717/150‑1 (MQ=255)
                                                                                                               aaTATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGt                                        <  1:3418343/150‑1 (MQ=255)
                                                                                                                 tatCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGttt                                      <  1:2145606/150‑1 (MQ=255)
                                                                                                                  atCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTc                                     <  2:1709883/150‑1 (MQ=255)
                                                                                                                  atCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTc                                     <  2:430491/150‑1 (MQ=255)
                                                                                                                    cgacgaTCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCAt                                   <  1:2543317/150‑1 (MQ=255)
                                                                                                                     gacgaTCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCAtt                                  >  2:3222798/1‑150 (MQ=255)
                                                                                                                     gacgaTCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCAtt                                  <  2:1237009/150‑1 (MQ=255)
                                                                                                                     gacgaTCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCAtt                                  >  1:1357030/1‑150 (MQ=255)
                                                                                                                     gacgaTCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCGATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCAtt                                  >  2:3259863/1‑150 (MQ=255)
                                                                                                                        gaTCTGGAAAAAATTTATAATCTTCTCTTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTATAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGat                               <  2:3672146/150‑1 (MQ=255)
                                                                                                                          tCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGatat                             <  2:4794781/150‑1 (MQ=255)
                                                                                                                          tCTGGAAAAAATTTATAAGCTTCTCGTCTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGatat                             <  1:439463/150‑1 (MQ=255)
                                                                                                                             ggAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGAtatata                          <  2:656926/150‑1 (MQ=255)
                                                                                                                              gAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATaa                         <  1:2947251/150‑1 (MQ=255)
                                                                                                                                 aaaaTTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATc                      >  2:4594587/1‑150 (MQ=255)
                                                                                                                                 aaaaTTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATc                      <  1:3951407/150‑1 (MQ=255)
                                                                                                                                   aaTTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCa                    >  1:1993700/1‑150 (MQ=255)
                                                                                                                                      ttATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAAt                 <  1:4532164/150‑1 (MQ=255)
                                                                                                                                        ataAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGa               >  1:2800488/1‑150 (MQ=255)
                                                                                                                                             cTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAATc          <  2:5079099/150‑1 (MQ=255)
                                                                                                                                                 tcGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAATCGTTa      <  2:5108778/150‑1 (MQ=255)
                                                                                                                                                 tcGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCACTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAATCGTTa      <  2:1742631/150‑1 (MQ=255)
                                                                                                                                                  cGTCTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAATCGTTAt     <  1:3013352/150‑1 (MQ=255)
                                                                                                                                                    tttCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAATCGTTATCa   <  1:2333292/150‑1 (MQ=255)
                                                                                                                                                    tttCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAATCGTTATCa   >  2:2923808/1‑150 (MQ=255)
                                                                                                                                                     ttCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGATTCATTGATATATAAATCCAAATGAAAATCGTTATCaa  >  2:4991410/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAATCGTTATCAA  >  CP009273/1899355‑1899653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A