Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,848,002 T→A 100% C102* (TGT→TGA)  fhlA → formate hydrogenlyase transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,848,0020TA99.7% 1014.0 / ‑5.1 305C102* (TGT→TGA) fhlAformate hydrogenlyase transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (153/151);  total (153/152)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 306 total aligned reads displayed.

AGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTC  >  CP009273/2847855‑2848146
                                                                                                                                                    |                                                                                                                                                
   gcAGACTCAACGTGCGTCTTATTCCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAAc                                                                                                                                             <  1:4674660/150‑1 (MQ=255)
   gcAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAAc                                                                                                                                             >  2:2950245/1‑150 (MQ=255)
    cAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAAcc                                                                                                                                            <  1:4511759/150‑1 (MQ=255)
    cAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAAcc                                                                                                                                            >  2:3722585/1‑150 (MQ=255)
      gACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTg                                                                                                                                          <  2:630855/150‑1 (MQ=255)
       aCTCAACGTGCTTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTgg                                                                                                                                         <  1:3798039/150‑1 (MQ=255)
       aCTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTgg                                                                                                                                         <  1:4423411/150‑1 (MQ=255)
        cTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGACACCTGGc                                                                                                                                        >  1:998038/1‑150 (MQ=255)
        cTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGc                                                                                                                                        >  1:2796235/1‑150 (MQ=255)
        cTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGc                                                                                                                                        >  1:4950041/1‑150 (MQ=255)
         tCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGcc                                                                                                                                       >  1:4269105/1‑150 (MQ=255)
           aaCGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGc                                                                                                                                     >  2:55404/1‑150 (MQ=255)
               tGCGTCTTTTTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGCAGACACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCATTTACGAAGAATTTTGAGAAACCTGGCCTCAGCt                                                                                                                                 <  1:1666405/150‑1 (MQ=255)
               tGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCt                                                                                                                                 <  2:840426/150‑1 (MQ=255)
                  gTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGa                                                                                                                              >  1:4750138/1‑150 (MQ=255)
                  gTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGa                                                                                                                              >  2:1386420/1‑150 (MQ=255)
                    cTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACg                                                                                                                            >  2:3280936/1‑150 (MQ=255)
                      tattaCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCa                                                                                                                          <  1:4626412/150‑1 (MQ=255)
                       attaCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAg                                                                                                                         >  2:397806/1‑150 (MQ=255)
                        ttaCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGACACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAgg                                                                                                                        <  2:2838088/150‑1 (MQ=255)
                        ttaCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAgg                                                                                                                        >  1:344336/1‑150 (MQ=255)
                         taCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGt                                                                                                                       >  2:74274/1‑150 (MQ=255)
                           cgcgtcgagtGAAAAAGACAACCCCATTAAATATGAAGACGAAACTTTTCTGTCACACGGTCCGGTACGCAGCA‑TTTTGTCTCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTgg                                                                                                                     <  1:79989/150‑1 (MQ=255)
                                  cgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAc                                                                                                              <  2:4047364/150‑1 (MQ=255)
                                    tGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAccc                                                                                                            >  2:1701691/1‑150 (MQ=255)
                                     gAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCa                                                                                                           >  2:1069508/1‑150 (MQ=255)
                                       aaaaGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCaaa                                                                                                         >  2:5045983/1‑150 (MQ=255)
                                        aaaGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCaaaa                                                                                                        <  1:3102799/150‑1 (MQ=255)
                                          aGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAAtt                                                                                                      >  2:1466656/1‑150 (MQ=255)
                                          aGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAAtt                                                                                                      <  2:4968344/150‑1 (MQ=255)
                                          aGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAAtt                                                                                                      >  1:2005265/1‑150 (MQ=255)
                                                ccccATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCa                                                                                                <  1:704247/150‑1 (MQ=255)
                                                ccccATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCa                                                                                                <  2:5293376/150‑1 (MQ=255)
                                                 cccATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCAc                                                                                               <  2:1485516/150‑1 (MQ=255)
                                                   cATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTa                                                                                             >  2:2721899/1‑150 (MQ=255)
                                                    aTTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTAt                                                                                            >  2:5082022/1‑150 (MQ=255)
                                                    aTTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTAt                                                                                            <  2:5213167/150‑1 (MQ=255)
                                                     ttAAATATGCAGACGACACTGTACTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTAtt                                                                                           <  1:2731182/150‑1 (MQ=255)
                                                     ttAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTAtt                                                                                           >  2:2067286/1‑150 (MQ=255)
                                                      tAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTg                                                                                          <  1:1085451/150‑1 (MQ=255)
                                                       aaaTATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGc                                                                                         >  1:1745267/1‑150 (MQ=255)
                                                        aaTATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGcc                                                                                        >  1:4563539/1‑150 (MQ=255)
                                                        aaTATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGcc                                                                                        >  2:729336/1‑150 (MQ=255)
                                                         atatGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCt                                                                                       >  2:1856910/1‑150 (MQ=255)
                                                          tatGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTg                                                                                      >  2:4135165/1‑150 (MQ=255)
                                                          tatGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTg                                                                                      >  1:1258313/1‑150 (MQ=255)
                                                           atGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGa                                                                                     >  2:2442726/1‑150 (MQ=255)
                                                            tGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGAt                                                                                    >  2:2888454/1‑150 (MQ=255)
                                                            tGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGAt                                                                                    <  2:935023/150‑1 (MQ=255)
                                                             gAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATg                                                                                   <  1:1832437/150‑1 (MQ=255)
                                                              aaGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATg                                                                                   <  1:3198187/150‑1 (MQ=255)
                                                              aaGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGc                                                                                  >  1:3407080/1‑150 (MQ=255)
                                                              aaGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGc                                                                                  >  2:2402329/1‑150 (MQ=255)
                                                               aGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGTCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGcc                                                                                 >  2:4859890/1‑150 (MQ=255)
                                                                gACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCa                                                                                <  2:4112702/150‑1 (MQ=255)
                                                                  cGAAACTGTTCTGGCACACGGTCCGGTACGCTGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACt                                                                              <  2:437390/150‑1 (MQ=255)
                                                                   gAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTg                                                                             >  1:1210778/1‑150 (MQ=255)
                                                                    aaaCTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTgg                                                                            >  2:233152/1‑150 (MQ=255)
                                                                     aaCTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTggc                                                                           >  2:3089835/1‑150 (MQ=255)
                                                                     aaCTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTggc                                                                           <  1:2141058/150‑1 (MQ=255)
                                                                     aaCTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTggc                                                                           <  1:3042883/150‑1 (MQ=255)
                                                                           tCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGa                                                                     <  1:4078829/150‑1 (MQ=255)
                                                                                cacaCGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGc                                                                <  1:2165400/150‑1 (MQ=255)
                                                                                 acacGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCa                                                               >  1:643247/1‑150 (MQ=255)
                                                                                 acacGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCa                                                               >  1:5183807/1‑150 (MQ=255)
                                                                                  cacGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCat                                                              <  1:911274/150‑1 (MQ=255)
                                                                                        ccGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAttttt                                                        >  2:2142573/1‑150 (MQ=255)
                                                                                        ccGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAttttt                                                        <  1:3505839/150‑1 (MQ=255)
                                                                                        acGGTACGCAGCA‑TTGTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTTGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAttttt                                                        <  1:1842704/149‑1 (MQ=255)
                                                                                          ggTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtgg                                                      >  1:1071064/1‑150 (MQ=255)
                                                                                           gTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtggt                                                     <  1:4159118/150‑1 (MQ=255)
                                                                                            tACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtggtg                                                    >  2:4797143/1‑150 (MQ=255)
                                                                                             aCGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtggtgg                                                   <  1:4377906/150‑1 (MQ=255)
                                                                                                cagca‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCATAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCtg                                                <  2:1315493/150‑1 (MQ=255)
                                                                                                cagca‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCtg                                                <  2:4640635/150‑1 (MQ=255)
                                                                                                  gca‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCtgtg                                              <  1:4984997/150‑1 (MQ=255)
                                                                                                      ttttGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAAt                                           <  1:3823307/150‑1 (MQ=255)
                                                                                                       tttGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAAtt                                          >  1:5079669/1‑150 (MQ=255)
                                                                                                       tttGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAAtt                                          >  1:2435336/1‑150 (MQ=255)
                                                                                                          gTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTAt                                       >  2:4697480/1‑150 (MQ=255)
                                                                                                          gTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTAt                                       >  1:4582997/1‑150 (MQ=255)
                                                                                                           tCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTAtt                                      <  1:3348700/150‑1 (MQ=255)
                                                                                                              cgcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGt                                   >  1:357710/1‑150 (MQ=255)
                                                                                                              cgcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGt                                   <  1:2048843/150‑1 (MQ=255)
                                                                                                                 ccTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTAt                                <  1:2884320/150‑1 (MQ=255)
                                                                                                                 ccTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTAt                                <  1:1346148/150‑1 (MQ=255)
                                                                                                                   tGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGTTGGCTGTGAATTTATTCGTTATGa                              >  2:3349574/1‑150 (MQ=255)
                                                                                                                    gATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGAc                             <  1:3280936/150‑1 (MQ=255)
                                                                                                                    gATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGAc                             <  1:4905822/150‑1 (MQ=255)
                                                                                                                        cGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATc                         <  2:49086/150‑1 (MQ=255)
                                                                                                                             cATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                    <  1:1726050/150‑1 (MQ=255)
                                                                                                                                tGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTgg                 >  2:115922/1‑150 (MQ=255)
                                                                                                                                    gTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCg             >  2:2568490/1‑150 (MQ=255)
                                                                                                                                      tACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaa           <  2:4502526/150‑1 (MQ=255)
                                                                                                                                      tACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaa           >  1:2293685/1‑150 (MQ=255)
                                                                                                                                      tACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaa           >  2:3494897/1‑150 (MQ=255)
                                                                                                                                          aagaaTTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAag       >  2:1641659/1‑150 (MQ=255)
                                                                                                                                          aagaaTTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAag       <  2:2712086/150‑1 (MQ=255)
                                                                                                                                           agaaTTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAaga      >  2:2271136/1‑150 (MQ=255)
                                                                                                                                               ttttGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTc  <  1:2624196/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
AGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTC  >  CP009273/2847855‑2848146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCG  >  CP009273/2847854‑2848151
                                                                                                                                                     |                                                                                                                                                     
AAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAG                                                                                                                                                      <  SRR19973062.2421307/150‑1 (MQ=60)
AAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAG                                                                                                                                                      >  SRR19973062.2561958/1‑150 (MQ=60)
AAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAG                                                                                                                                                      >  SRR19973062.5219284/1‑150 (MQ=60)
AAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAG                                                                                                                                                      >  SRR19973062.2561979/1‑150 (MQ=60)
AAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAG                                                                                                                                                      <  SRR19973062.1210975/150‑1 (MQ=60)
AAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAG                                                                                                                                                      >  SRR19973062.5219592/1‑150 (MQ=60)
    GCAGACTCAACGTGCGTCTTATTCCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAAC                                                                                                                                                  <  SRR19973062.4699106/150‑1 (MQ=60)
     CAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACC                                                                                                                                                 <  SRR19973062.4535164/150‑1 (MQ=60)
     CAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACC                                                                                                                                                 >  SRR19973062.4452813/1‑150 (MQ=60)
     CAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACC                                                                                                                                                 >  SRR19973062.4452921/1‑150 (MQ=60)
     CAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACC                                                                                                                                                 <  SRR19973062.1767449/150‑1 (MQ=60)
     CAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACC                                                                                                                                                 <  SRR19973062.865905/150‑1 (MQ=60)
      AGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCT                                                                                                                                                <  SRR19973062.3837072/150‑1 (MQ=60)
      AGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCGGATACGCTGCATTGCAGTTACGAAGAATTTGGAGAAACCT                                                                                                                                                >  SRR19973062.4994538/1‑150 (MQ=60)
      AGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCAATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCT                                                                                                                                                >  SRR19973062.3171423/1‑150 (MQ=60)
        ACTCAACGTGCTTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGG                                                                                                                                              <  SRR19973062.3817485/150‑1 (MQ=60)
        ACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATTAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGG                                                                                                                                              <  SRR19973062.1843293/150‑1 (MQ=60)
        ACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGG                                                                                                                                              <  SRR19973062.4217851/150‑1 (MQ=60)
        ACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGG                                                                                                                                              <  SRR19973062.2551971/150‑1 (MQ=60)
        ACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGG                                                                                                                                              <  SRR19973062.4446009/150‑1 (MQ=60)
        AATCAACGTGCGTCTTATTACGCGTCGCGTTAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACTCGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGG                                                                                                                                              <  SRR19973062.1843215/150‑1 (MQ=60)
         CTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGACACCTGGC                                                                                                                                             >  SRR19973062.1002690/1‑150 (MQ=60)
         CTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGC                                                                                                                                             >  SRR19973062.4976023/1‑150 (MQ=60)
         CTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGC                                                                                                                                             >  SRR19973062.2811025/1‑150 (MQ=60)
          TCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCC                                                                                                                                            >  SRR19973062.4291200/1‑150 (MQ=60)
          TCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCC                                                                                                                                            <  SRR19973062.761690/150‑1 (MQ=60)
              CGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAG                                                                                                                                        <  SRR19973062.2247492/150‑1 (MQ=60)
               GTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGC                                                                                                                                       >  SRR19973062.1250506/1‑150 (MQ=60)
                TGCGTCTTTTTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGCAGACACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCATTTACGAAGAATTTTGAGAAACCTGGCCTCAGCT                                                                                                                                      <  SRR19973062.1674588/150‑1 (MQ=60)
                 GCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTG                                                                                                                                     >  SRR19973062.3365918/1‑150 (MQ=60)
                   GTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGA                                                                                                                                   >  SRR19973062.128182/1‑150 (MQ=60)
                   GTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGA                                                                                                                                   >  SRR19973062.4535171/1‑150 (MQ=60)
                   GTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGA                                                                                                                                   >  SRR19973062.4774867/1‑150 (MQ=60)
                    TCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGAC                                                                                                                                  <  SRR19973062.5167246/150‑1 (MQ=60)
                     CTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACG                                                                                                                                 >  SRR19973062.2035050/1‑150 (MQ=60)
                       TATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCA                                                                                                                               <  SRR19973062.4650616/150‑1 (MQ=60)
                       TATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCA                                                                                                                               <  SRR19973062.4650608/150‑1 (MQ=60)
                       TATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCA                                                                                                                               <  SRR19973062.4579046/150‑1 (MQ=60)
                         TTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGG                                                                                                                             >  SRR19973062.345917/1‑150 (MQ=60)
                         TTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGG                                                                                                                             <  SRR19973062.2153468/150‑1 (MQ=60)
                          TACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGT                                                                                                                            >  SRR19973062.312824/1‑150 (MQ=60)
                           ACGCGTCGCGTGAAAAAGACACCCCCATTCACTCTGAAGACGAACCTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTG                                                                                                                           <  SRR19973062.1964791/150‑1 (MQ=60)
                            CGCGTCGAGTGAAAAAGACAACCCCATTAAATATGAAGACGAAACTTTTCTGTCACACGGTCCGGTACGCAGCA‑TTTTGTCTCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGG                                                                                                                          <  SRR19973062.80513/150‑1 (MQ=60)
                             GCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGG                                                                                                                         <  SRR19973062.1730309/150‑1 (MQ=60)
                             GCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGG                                                                                                                         >  SRR19973062.3497877/1‑150 (MQ=60)
                               GTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCT                                                                                                                       <  SRR19973062.4704541/150‑1 (MQ=60)
                               GTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCT                                                                                                                       >  SRR19973062.4178430/1‑150 (MQ=60)
                                 CGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTAT                                                                                                                     >  SRR19973062.4953233/1‑150 (MQ=60)
                                  GCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATA                                                                                                                    <  SRR19973062.4925483/150‑1 (MQ=60)
                                        AAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAA                                                                                                              >  SRR19973062.4150404/1‑150 (MQ=60)
                                         AAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAA                                                                                                             <  SRR19973062.3118787/150‑1 (MQ=60)
                                          AAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAAT                                                                                                            >  SRR19973062.2726352/1‑150 (MQ=60)
                                           AGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATT                                                                                                           >  SRR19973062.2015571/1‑150 (MQ=60)
                                            GACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTT                                                                                                          >  SRR19973062.3012449/1‑150 (MQ=60)
                                            GACACCCCCATNAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTT                                                                                                          >  SRR19973062.2778062/1‑150 (MQ=60)
                                              CACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGG                                                                                                        <  SRR19973062.4337144/150‑1 (MQ=60)
                                               ACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGT                                                                                                       >  SRR19973062.956108/1‑150 (MQ=60)
                                                 CCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCA                                                                                                     <  SRR19973062.707496/150‑1 (MQ=60)
                                                 CCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCA                                                                                                     <  SRR19973062.707487/150‑1 (MQ=60)
                                                  CCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCAC                                                                                                    >  SRR19973062.2464637/1‑150 (MQ=60)
                                                  CCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCAC                                                                                                    >  SRR19973062.3426525/1‑150 (MQ=60)
                                                    CATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTA                                                                                                  <  SRR19973062.4073770/150‑1 (MQ=60)
                                                     ATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTAT                                                                                                 <  SRR19973062.4430376/150‑1 (MQ=60)
                                                      TTAAATATGCAGACGACACTGTACTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATT                                                                                                <  SRR19973062.2745564/150‑1 (MQ=60)
                                                       TAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTG                                                                                               <  SRR19973062.1090563/150‑1 (MQ=60)
                                                       TAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTG                                                                                               >  SRR19973062.3070396/1‑150 (MQ=60)
                                                       TAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTG                                                                                               >  SRR19973062.3070453/1‑150 (MQ=60)
                                                        AAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGC                                                                                              >  SRR19973062.1196934/1‑150 (MQ=60)
                                                        AAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGC                                                                                              >  SRR19973062.4343293/1‑150 (MQ=60)
                                                        AAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGC                                                                                              >  SRR19973062.1753749/1‑150 (MQ=60)
                                                         AATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCC                                                                                             >  SRR19973062.4587373/1‑150 (MQ=60)
                                                           TATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTG                                                                                           >  SRR19973062.1264122/1‑150 (MQ=60)
                                                           TATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTG                                                                                           >  SRR19973062.4932183/1‑150 (MQ=60)
                                                              GAAGCCGCAACGGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATG                                                                                        <  SRR19973062.3214883/150‑1 (MQ=60)
                                                              GAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATG                                                                                        <  SRR19973062.1841511/150‑1 (MQ=60)
                                                              GAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATG                                                                                        <  SRR19973062.4420713/150‑1 (MQ=60)
                                                              GAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATG                                                                                        <  SRR19973062.2166683/150‑1 (MQ=60)
                                                               AAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATG                                                                                        <  SRR19973062.3214789/150‑1 (MQ=60)
                                                               AAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGC                                                                                       >  SRR19973062.3415773/1‑150 (MQ=60)
                                                               AAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGC                                                                                       >  SRR19973062.3424539/1‑150 (MQ=60)
                                                                   CGAGACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACT                                                                                   >  SRR19973062.842556/1‑150 (MQ=60)
                                                                   CGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACT                                                                                   >  SRR19973062.2376802/1‑150 (MQ=60)
                                                                    GAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTG                                                                                  >  SRR19973062.1216418/1‑150 (MQ=60)
                                                                     AAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGG                                                                                 >  SRR19973062.4671315/1‑150 (MQ=60)
                                                                     AAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGG                                                                                 >  SRR19973062.4672116/1‑150 (MQ=60)
                                                                     AAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGG                                                                                 >  SRR19973062.726940/1‑150 (MQ=60)
                                                                     AAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGG                                                                                 >  SRR19973062.1288655/1‑150 (MQ=60)
                                                                      AACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGC                                                                                <  SRR19973062.499012/150‑1 (MQ=60)
                                                                      AACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGC                                                                                <  SRR19973062.3058709/150‑1 (MQ=60)
                                                                      AACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGC                                                                                <  SRR19973062.2151921/150‑1 (MQ=60)
                                                                       ACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCG                                                                               >  SRR19973062.2659970/1‑150 (MQ=60)
                                                                        CTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGG                                                                              >  SRR19973062.4597612/1‑150 (MQ=60)
                                                                          GTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCG                                                                            >  SRR19973062.2094111/1‑150 (MQ=60)
                                                                          GTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCG                                                                            <  SRR19973062.5260781/150‑1 (MQ=60)
                                                                          GTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCG                                                                            >  SRR19973062.460123/1‑150 (MQ=60)
                                                                            TCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGA                                                                          <  SRR19973062.4099802/150‑1 (MQ=60)
                                                                            TCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGA                                                                          >  SRR19973062.1809123/1‑150 (MQ=60)
                                                                                 CACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC                                                                     >  SRR19973062.1751648/1‑150 (MQ=60)
                                                                                 CACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC                                                                     <  SRR19973062.2176345/150‑1 (MQ=60)
                                                                                  ACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA                                                                    >  SRR19973062.646252/1‑150 (MQ=60)
                                                                                  ACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA                                                                    >  SRR19973062.5211171/1‑150 (MQ=60)
                                                                                   CACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT                                                                   <  SRR19973062.915356/150‑1 (MQ=60)
                                                                                   CACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT                                                                   <  SRR19973062.2026239/150‑1 (MQ=60)
                                                                                   CACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT                                                                   <  SRR19973062.3625002/150‑1 (MQ=60)
                                                                                   CACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT                                                                   <  SRR19973062.915251/150‑1 (MQ=60)
                                                                                     CGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAT                                                                 <  SRR19973062.4249762/150‑1 (MQ=60)
                                                                                     CGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAT                                                                 <  SRR19973062.1463469/150‑1 (MQ=60)
                                                                                      GGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT                                                                >  SRR19973062.284303/1‑150 (MQ=60)
                                                                                       GTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTT                                                               >  SRR19973062.3538087/1‑150 (MQ=60)
                                                                                       GTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTT                                                               <  SRR19973062.1485417/150‑1 (MQ=60)
                                                                                         CCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTT                                                             <  SRR19973062.3523624/150‑1 (MQ=60)
                                                                                         ACGGTACGCAGCA‑TTGTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTTGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTT                                                             <  SRR19973062.1851893/150‑1 (MQ=60)
                                                                                           GGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGG                                                           >  SRR19973062.1076110/1‑150 (MQ=60)
                                                                                           GGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGG                                                           >  SRR19973062.1076134/1‑150 (MQ=60)
                                                                                           GGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGG                                                           >  SRR19973062.1076132/1‑150 (MQ=60)
                                                                                            GTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGT                                                          <  SRR19973062.4180693/150‑1 (MQ=60)
                                                                                              ACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGG                                                        <  SRR19973062.4400360/150‑1 (MQ=60)
                                                                                              ACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGG                                                        >  SRR19973062.5190847/1‑150 (MQ=60)
                                                                                               CGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGC                                                       <  SRR19973062.2433529/150‑1 (MQ=60)
                                                                                                  AGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGT                                                    >  SRR19973062.4821758/1‑150 (MQ=60)
                                                                                                   GCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTG                                                   <  SRR19973062.5011321/150‑1 (MQ=60)
                                                                                                   GCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTG                                                   <  SRR19973062.5011355/150‑1 (MQ=60)
                                                                                                   GCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTG                                                   <  SRR19973062.5011823/150‑1 (MQ=60)
                                                                                                   GCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTG                                                   >  SRR19973062.2094415/1‑150 (MQ=60)
                                                                                                       TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAAT                                                <  SRR19973062.3842870/150‑1 (MQ=60)
                                                                                                        TTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATT                                               >  SRR19973062.5106570/1‑150 (MQ=60)
                                                                                                        TTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATT                                               >  SRR19973062.2448410/1‑150 (MQ=60)
                                                                                                           GTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTAT                                            <  SRR19973062.3410553/150‑1 (MQ=60)
                                                                                                           GTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAGATTTTTGGTGGCTGTGAATTTAT                                            >  SRR19973062.4606968/1‑150 (MQ=60)
                                                                                                            TCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATT                                           <  SRR19973062.3365957/150‑1 (MQ=60)
                                                                                                             CGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTC                                          <  SRR19973062.4330384/150‑1 (MQ=60)
                                                                                                               CGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGT                                        <  SRR19973062.2059337/150‑1 (MQ=60)
                                                                                                               CGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGT                                        >  SRR19973062.359330/1‑150 (MQ=60)
                                                                                                                GCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTT                                       <  SRR19973062.4647195/150‑1 (MQ=60)
                                                                                                                 CCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTA                                      >  SRR19973062.3020101/1‑150 (MQ=60)
                                                                                                                  CCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTAT                                     <  SRR19973062.1352237/150‑1 (MQ=60)
                                                                                                                  CCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTAT                                     <  SRR19973062.2899550/150‑1 (MQ=60)
                                                                                                                   CTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATG                                    >  SRR19973062.1926049/1‑150 (MQ=60)
                                                                                                                    TGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGA                                   <  SRR19973062.3352461/150‑1 (MQ=60)
                                                                                                                    TGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGA                                   <  SRR19973062.662011/150‑1 (MQ=60)
                                                                                                                     GATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGAC                                  <  SRR19973062.2814183/150‑1 (MQ=60)
                                                                                                                     GATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGAC                                  <  SRR19973062.3297936/150‑1 (MQ=60)
                                                                                                                     GATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGAC                                  <  SRR19973062.4931161/150‑1 (MQ=60)
                                                                                                                      ATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACG                                 >  SRR19973062.864679/1‑150 (MQ=60)
                                                                                                                       TACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGA                                <  SRR19973062.1256986/150‑1 (MQ=60)
                                                                                                                        ACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGAT                               <  SRR19973062.3558145/150‑1 (MQ=60)
                                                                                                                         CGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATC                              <  SRR19973062.3621335/150‑1 (MQ=60)
                                                                                                                         CGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATC                              <  SRR19973062.4699271/150‑1 (MQ=60)
                                                                                                                            TGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCC                           <  SRR19973062.3243958/150‑1 (MQ=60)
                                                                                                                              CATTTCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCC                         <  SRR19973062.3469512/150‑1 (MQ=60)
                                                                                                                              CATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCC                         <  SRR19973062.5332709/150‑1 (MQ=60)
                                                                                                                              CATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCC                         >  SRR19973062.3841917/1‑150 (MQ=60)
                                                                                                                              CATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCC                         >  SRR19973062.4049519/1‑150 (MQ=60)
                                                                                                                              CATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCC                         <  SRR19973062.1734461/150‑1 (MQ=60)
                                                                                                                              CATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCC                         >  SRR19973062.2208340/1‑150 (MQ=60)
                                                                                                                               ATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCT                        >  SRR19973062.1752045/1‑150 (MQ=60)
                                                                                                                                      TTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGA                 >  SRR19973062.3437224/1‑150 (MQ=60)
                                                                                                                                       TACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAA                >  SRR19973062.2305586/1‑150 (MQ=60)
                                                                                                                                           AAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAG            <  SRR19973062.1716437/150‑1 (MQ=60)
                                                                                                                                              AATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGT         <  SRR19973062.1184809/150‑1 (MQ=60)
                                                                                                                                               ATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTT        >  SRR19973062.276710/1‑150 (MQ=60)
                                                                                                                                                TTTTGAGAAACCTGGCCGCAGCTGGTCGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGATAAAGAGTTC       <  SRR19973062.2638064/150‑1 (MQ=60)
                                                                                                                                                TTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTC       <  SRR19973062.2638033/150‑1 (MQ=60)
                                                                                                                                                   TGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAAT    <  SRR19973062.1419611/150‑1 (MQ=60)
                                                                                                                                                   TGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAAT    >  SRR19973062.1049334/1‑150 (MQ=60)
                                                                                                                                                    GAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATC   <  SRR19973062.3883850/150‑1 (MQ=60)
                                                                                                                                                     AGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCG  <  SRR19973062.3797676/150‑1 (MQ=60)
                                                                                                                                                     AGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATATGGTCACTATTGCCTGATGCCACTCGCCGCGGAAGGGCATAATTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTACAATCG  >  SRR19973062.2803006/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCA‑TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCG  >  CP009273/2847854‑2848151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑