Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F361 I0 R1 1353 250.7 8230166 96.0% 7900959 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,848,002 T→A 100% C102* (TGT→TGA)  fhlA → formate hydrogenlyase transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,848,0020TA98.3% 789.2 / 1.9 239C102* (TGT→TGA) fhlAformate hydrogenlyase transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base A (112/123);  total (115/124)
Fisher's exact test for biased strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 241 total aligned reads displayed.

GCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCA  >  CP009273/2847856‑2848147
                                                                                                                                                  |                                                                                                                                                 
  gcAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGaaa                                                                                                                                               >  1:2636672/1‑149 (MQ=255)
   cAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAAc                                                                                                                                              >  2:2344428/1‑149 (MQ=255)
   cAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAAc                                                                                                                                              >  2:3015144/1‑149 (MQ=255)
     gACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCt                                                                                                                                            >  2:1897441/1‑149 (MQ=255)
     gACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCt                                                                                                                                            <  2:701577/149‑1 (MQ=255)
     gACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCt                                                                                                                                            <  2:392264/149‑1 (MQ=255)
       cTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTgg                                                                                                                                          <  1:4102510/149‑1 (MQ=255)
              tGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGc                                                                                                                                   >  2:857597/1‑149 (MQ=255)
              tGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGc                                                                                                                                   >  2:2916829/1‑149 (MQ=255)
                     tattaCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGc                                                                                                                            <  1:1637307/149‑1 (MQ=255)
                      attaCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCa                                                                                                                           <  1:3384359/149‑1 (MQ=255)
                         aCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGt                                                                                                                        <  2:3873670/149‑1 (MQ=255)
                           gcgtcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTgg                                                                                                                      >  1:823485/1‑149 (MQ=255)
                           gcgtcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTgg                                                                                                                      >  1:2589747/1‑149 (MQ=255)
                             gtcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGc                                                                                                                    <  1:770290/149‑1 (MQ=255)
                                 cgtGAAAAAGACACCCCCATTAAATATGAAGACGACACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCtata                                                                                                                <  1:465493/149‑1 (MQ=255)
                                  gtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAc                                                                                                               <  1:1734447/149‑1 (MQ=255)
                                  gtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAc                                                                                                               <  1:489039/149‑1 (MQ=255)
                                  gtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAc                                                                                                               >  2:188779/1‑149 (MQ=255)
                                  gtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTACGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAc                                                                                                               >  2:188676/1‑149 (MQ=255)
                                   tGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAcc                                                                                                              <  1:2625202/149‑1 (MQ=255)
                                     aaaaaGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGAATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAccaa                                                                                                            >  2:4020849/1‑147 (MQ=255)
                                      aaaaGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCaa                                                                                                           >  1:3580472/1‑149 (MQ=255)
                                        aaGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCaaaa                                                                                                         >  2:3239411/1‑149 (MQ=255)
                                         aGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAAt                                                                                                        <  1:2510430/149‑1 (MQ=255)
                                         aGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAAt                                                                                                        <  2:2386154/149‑1 (MQ=255)
                                            cacCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTg                                                                                                     >  1:3213075/1‑149 (MQ=255)
                                             acCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTgg                                                                                                    <  1:3978016/149‑1 (MQ=255)
                                              cccccATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGt                                                                                                   >  2:1121337/1‑149 (MQ=255)
                                              cccccATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGt                                                                                                   >  1:1991080/1‑149 (MQ=255)
                                                cccATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCa                                                                                                 >  1:368960/1‑149 (MQ=255)
                                                 ccATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCAc                                                                                                >  1:2398826/1‑149 (MQ=255)
                                                 ccATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCAc                                                                                                >  1:400128/1‑149 (MQ=255)
                                                  cATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACt                                                                                               >  2:1137781/1‑149 (MQ=255)
                                                    ttAAATATGAAGACGACACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTAt                                                                                             <  2:3250174/149‑1 (MQ=255)
                                                     tAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTAtt                                                                                            <  2:2636784/149‑1 (MQ=255)
                                                      aaaTATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTg                                                                                           >  2:1455388/1‑149 (MQ=255)
                                                       aaTATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGc                                                                                          <  2:398443/149‑1 (MQ=255)
                                                       aaTATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGc                                                                                          <  1:249862/149‑1 (MQ=255)
                                                        atagGAAGACGAAACTGTTCGGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGcc                                                                                         <  2:3491640/149‑1 (MQ=255)
                                                           tGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGa                                                                                      >  1:3723511/1‑149 (MQ=255)
                                                           tGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGa                                                                                      >  1:311101/1‑149 (MQ=255)
                                                           tGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGa                                                                                      >  1:3857103/1‑149 (MQ=255)
                                                           tGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGa                                                                                      >  1:1936468/1‑149 (MQ=255)
                                                           tGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGa                                                                                      >  1:1677742/1‑149 (MQ=255)
                                                            gAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGAt                                                                                     >  2:593639/1‑149 (MQ=255)
                                                             aaGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATg                                                                                    >  1:23680/1‑149 (MQ=255)
                                                              acaCGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGc                                                                                   <  1:9829/147‑1 (MQ=255)
                                                              aGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGc                                                                                   <  1:45765/149‑1 (MQ=255)
                                                               gACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGcc                                                                                  <  2:1641700/149‑1 (MQ=255)
                                                                  gAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACt                                                                               <  2:2965593/149‑1 (MQ=255)
                                                                   aaaCTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTg                                                                              <  2:2701115/149‑1 (MQ=255)
                                                                    aaCTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTgg                                                                             >  1:1240933/1‑149 (MQ=255)
                                                                    aaCTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTgg                                                                             >  2:3310395/1‑149 (MQ=255)
                                                                          tCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGcggcgg                                                                       <  1:1048486/149‑1 (MQ=255)
                                                                          tCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGcggcgg                                                                       <  2:406546/149‑1 (MQ=255)
                                                                             ggCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAg                                                                    <  2:2742466/149‑1 (MQ=255)
                                                                              gCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAgg                                                                   <  2:3603547/149‑1 (MQ=255)
                                                                               cacaCGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAggg                                                                  <  1:2679392/149‑1 (MQ=255)
                                                                               cacaCGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAggg                                                                  <  1:2859148/149‑1 (MQ=255)
                                                                                 cacGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCa                                                                <  2:425265/149‑1 (MQ=255)
                                                                                   cGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCata                                                              <  1:3180583/149‑1 (MQ=255)
                                                                                    ggTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCatat                                                             >  2:3543758/1‑149 (MQ=255)
                                                                                       ccGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGTCTATACCCAAATTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAtttt                                                          <  1:1551452/149‑1 (MQ=255)
                                                                                         ggTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtg                                                        >  1:2757442/1‑149 (MQ=255)
                                                                                         ggTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtg                                                        <  1:2946607/149‑1 (MQ=255)
                                                                                           tACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtggt                                                      >  2:2594377/1‑149 (MQ=255)
                                                                                            aCGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtggtg                                                     <  1:2220796/149‑1 (MQ=255)
                                                                                            aCGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAggg                                                                  >  2:1752138/1‑136 (MQ=255)
                                                                                            aCGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAggg                                                                  <  1:1751530/136‑1 (MQ=255)
                                                                                            aCGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtggtg                                                     <  1:726718/149‑1 (MQ=255)
                                                                                            aCGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtggtg                                                     <  2:3997678/149‑1 (MQ=255)
                                                                                            aCGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtggtg                                                     <  2:1738501/149‑1 (MQ=255)
                                                                                             cGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtggtgg                                                    <  1:3015144/149‑1 (MQ=255)
                                                                                               cagcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCt                                                  <  2:484059/149‑1 (MQ=255)
                                                                                               cagcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCt                                                  <  2:485711/149‑1 (MQ=255)
                                                                                                agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCCCTGGCGGCGGAAGGGCATATTTTTGGTGGCtg                                                 >  2:57366/1‑149 (MQ=255)
                                                                                                         tCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTAt                                        <  1:2061063/149‑1 (MQ=255)
                                                                                                             gcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGt                                    <  2:2024968/149‑1 (MQ=255)
                                                                                                             gcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGt                                    <  2:3814591/149‑1 (MQ=255)
                                                                                                               ccTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTa                                  <  1:3775745/149‑1 (MQ=255)
                                                                                                                   aTACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGAc                              <  2:838897/149‑1 (MQ=255)
                                                                                                                    tACGCTGCATTGCACTTACGAACAATATTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGTTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACg                             <  1:639145/149‑1 (MQ=255)
                                                                                                                       gCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATc                          <  1:3605386/149‑1 (MQ=255)
                                                                                                                          gCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAATGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGcc                       >  1:1014667/1‑149 (MQ=255)
                                                                                                                             ttGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCt                    <  1:233103/149‑1 (MQ=255)
                                                                                                                                cAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGa                 <  1:1457579/149‑1 (MQ=255)
                                                                                                                                 aGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAg                >  2:587097/1‑149 (MQ=255)
                                                                                                                                    tACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGa             >  1:773526/1‑149 (MQ=255)
                                                                                                                                    tACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGa             >  2:1164744/1‑149 (MQ=255)
                                                                                                                                     aCGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaa            >  1:517927/1‑149 (MQ=255)
                                                                                                                                       gaagaaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaaaa          >  1:2823729/1‑149 (MQ=255)
                                                                                                                                       gaagaaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaaaa          >  1:1368022/1‑149 (MQ=255)
                                                                                                                                          gaaTTATGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCTAAAAaga       <  2:743698/149‑1 (MQ=255)
                                                                                                                                           aaTTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAagag      >  2:206638/1‑149 (MQ=255)
                                                                                                                                            aTTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGt     <  2:2531602/149‑1 (MQ=255)
                                                                                                                                             ttttGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGTATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGtt    <  1:2151498/149‑1 (MQ=255)
                                                                                                                                             ttttGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGtt    >  2:3211270/1‑149 (MQ=255)
                                                                                                                                               ttGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCa  <  1:735844/149‑1 (MQ=255)
                                                                                                                                               ttGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCa  >  2:1064605/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCA  >  CP009273/2847856‑2848147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCG  >  CP009273/2847855‑2848151
                                                                                                                                                   |                                                                                                                                                     
AGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGA                                                                                                                                                     >  SRR19973068.3816219/1‑150 (MQ=60)
AGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGA                                                                                                                                                     >  SRR19973068.1461895/1‑150 (MQ=60)
  CGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAA                                                                                                                                                   >  SRR19973068.2653233/1‑150 (MQ=60)
  CGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAA                                                                                                                                                   >  SRR19973068.2653345/1‑150 (MQ=60)
       ACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGG                                                                                                                                              <  SRR19973068.4007195/150‑1 (MQ=60)
       ACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGG                                                                                                                                              <  SRR19973068.542953/150‑1 (MQ=60)
        CTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGC                                                                                                                                             <  SRR19973068.4127075/150‑1 (MQ=60)
          CAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCG                                                                                                                                           <  SRR19973068.71915/150‑1 (MQ=60)
           AACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGC                                                                                                                                          >  SRR19973068.2525787/1‑150 (MQ=60)
             CGTGCGTCTTATTCCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAG                                                                                                                                        <  SRR19973068.361116/150‑1 (MQ=60)
             CGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAG                                                                                                                                        <  SRR19973068.3178568/150‑1 (MQ=60)
                 CGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGG                                                                                                                                    >  SRR19973068.2398808/1‑150 (MQ=60)
                 CGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGG                                                                                                                                    >  SRR19973068.128895/1‑150 (MQ=60)
                  GTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGA                                                                                                                                   >  SRR19973068.294256/1‑150 (MQ=60)
                      TATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCA                                                                                                                               <  SRR19973068.1647969/150‑1 (MQ=60)
                      TATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCA                                                                                                                               <  SRR19973068.781663/150‑1 (MQ=60)
                      TATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCA                                                                                                                               <  SRR19973068.3912191/150‑1 (MQ=60)
                       ATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAG                                                                                                                              <  SRR19973068.3404878/150‑1 (MQ=60)
                         TACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGT                                                                                                                            <  SRR19973068.491179/150‑1 (MQ=60)
                         TACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGT                                                                                                                            <  SRR19973068.491173/150‑1 (MQ=60)
                         TACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGT                                                                                                                            <  SRR19973068.230498/150‑1 (MQ=60)
                           CGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGG                                                                                                                          >  SRR19973068.2606102/1‑150 (MQ=60)
                           CGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGG                                                                                                                          >  SRR19973068.829084/1‑150 (MQ=60)
                              GTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCT                                                                                                                       <  SRR19973068.775961/150‑1 (MQ=60)
                              GTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCT                                                                                                                       <  SRR19973068.775499/150‑1 (MQ=60)
                                  CGTGAAAAAGACACCCCCATTAAATATGAAGACGACACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAC                                                                                                                   <  SRR19973068.468776/150‑1 (MQ=60)
                                   GTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACC                                                                                                                  >  SRR19973068.2153703/1‑150 (MQ=60)
                                   GTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACC                                                                                                                  <  SRR19973068.492469/150‑1 (MQ=60)
                                   GTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACC                                                                                                                  <  SRR19973068.1744116/150‑1 (MQ=60)
                                   GTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACC                                                                                                                  <  SRR19973068.1745431/150‑1 (MQ=60)
                                   GTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACC                                                                                                                  <  SRR19973068.1817656/150‑1 (MQ=60)
                                    TGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCC                                                                                                                 <  SRR19973068.2641595/150‑1 (MQ=60)
                                    TGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCC                                                                                                                 <  SRR19973068.2641713/150‑1 (MQ=60)
                                    TGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCC                                                                                                                 <  SRR19973068.2641918/150‑1 (MQ=60)
                                      AAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAA                                                                                                               >  SRR19973068.1543157/1‑150 (MQ=60)
                                      AAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAA                                                                                                               >  SRR19973068.3602225/1‑150 (MQ=60)
                                        AAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAA                                                                                                             >  SRR19973068.435224/1‑150 (MQ=60)
                                         AAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAAT                                                                                                            >  SRR19973068.2547704/1‑150 (MQ=60)
                                         AAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAAT                                                                                                            >  SRR19973068.2547695/1‑150 (MQ=60)
                                          AGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATT                                                                                                           <  SRR19973068.444012/150‑1 (MQ=60)
                                          AGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATT                                                                                                           <  SRR19973068.2526412/150‑1 (MQ=60)
                                            ACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTG                                                                                                         >  SRR19973068.1168577/1‑150 (MQ=60)
                                            ACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTG                                                                                                         >  SRR19973068.4033271/1‑150 (MQ=60)
                                            ACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTG                                                                                                         >  SRR19973068.3232823/1‑150 (MQ=60)
                                              ACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGT                                                                                                       <  SRR19973068.4002072/150‑1 (MQ=60)
                                              ACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGT                                                                                                       >  SRR19973068.2004222/1‑150 (MQ=60)
                                                CCCCATTACATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCA                                                                                                     <  SRR19973068.1990541/150‑1 (MQ=60)
                                                CCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCA                                                                                                     >  SRR19973068.371592/1‑150 (MQ=60)
                                                CCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCA                                                                                                     >  SRR19973068.371434/1‑150 (MQ=60)
                                                 CCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCAC                                                                                                    >  SRR19973068.402861/1‑150 (MQ=60)
                                                 CCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCAC                                                                                                    >  SRR19973068.2414080/1‑150 (MQ=60)
                                                 CCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCAC                                                                                                    >  SRR19973068.402933/1‑150 (MQ=60)
                                                   CATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTA                                                                                                  >  SRR19973068.734744/1‑150 (MQ=60)
                                                     TTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATT                                                                                                >  SRR19973068.3308019/1‑150 (MQ=60)
                                                     TTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAACCAAAATTTGGTCACTATT                                                                                                <  SRR19973068.2645239/150‑1 (MQ=60)
                                                       AAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGC                                                                                              >  SRR19973068.748333/1‑150 (MQ=60)
                                                        AATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCC                                                                                             <  SRR19973068.251675/150‑1 (MQ=60)
                                                           ATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGA                                                                                          >  SRR19973068.3745866/1‑150 (MQ=60)
                                                           ATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGA                                                                                          >  SRR19973068.313148/1‑150 (MQ=60)
                                                           ATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGA                                                                                          >  SRR19973068.1688549/1‑150 (MQ=60)
                                                           ATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGA                                                                                          >  SRR19973068.1949337/1‑150 (MQ=60)
                                                           ATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGA                                                                                          >  SRR19973068.3880391/1‑150 (MQ=60)
                                                             GAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATG                                                                                        >  SRR19973068.23995/1‑150 (MQ=60)
                                                             GAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATG                                                                                        >  SRR19973068.24021/1‑150 (MQ=60)
                                                              AAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGC                                                                                       >  SRR19973068.1032799/1‑150 (MQ=60)
                                                               AGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCC                                                                                      <  SRR19973068.46337/150‑1 (MQ=60)
                                                               ACACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCC                                                                                      <  SRR19973068.9983/150‑1 (MQ=60)
                                                                    AAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGG                                                                                 >  SRR19973068.1249159/1‑150 (MQ=60)
                                                                      ACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCG                                                                               <  SRR19973068.1352954/150‑1 (MQ=60)
                                                                         GTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCG                                                                            <  SRR19973068.1700649/150‑1 (MQ=60)
                                                                           TCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGA                                                                          <  SRR19973068.1055263/150‑1 (MQ=60)
                                                                               GCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG                                                                      >  SRR19973068.985768/1‑150 (MQ=60)
                                                                                ctcttccgatctTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC                                                                     <  SRR19973068.1763367/138‑1 (MQ=60)
                                                                                ctcttccgatctTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC                                                                     <  SRR19973068.1762748/138‑1 (MQ=60)
                                                                                CACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC                                                                     <  SRR19973068.2876962/150‑1 (MQ=60)
                                                                                CACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC                                                                     <  SRR19973068.2696092/150‑1 (MQ=60)
                                                                                CACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC                                                                     <  SRR19973068.2876925/150‑1 (MQ=60)
                                                                                  CACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT                                                                   <  SRR19973068.1758130/150‑1 (MQ=60)
                                                                                    CGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAT                                                                 <  SRR19973068.3200158/150‑1 (MQ=60)
                                                                                        CCGGTAAGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGTCTATACCCAAATTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTT                                                             <  SRR19973068.1561666/150‑1 (MQ=60)
                                                                                         CGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTG                                                            >  SRR19973068.2774435/1‑150 (MQ=60)
                                                                                         CGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTG                                                            >  SRR19973068.2774313/1‑150 (MQ=60)
                                                                                         CGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTG                                                            >  SRR19973068.2774412/1‑150 (MQ=60)
                                                                                         CGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTG                                                            >  SRR19973068.2774377/1‑150 (MQ=60)
                                                                                          GGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGG                                                           <  SRR19973068.2964891/150‑1 (MQ=60)
                                                                                             ACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGG                                                        <  SRR19973068.2235314/150‑1 (MQ=60)
                                                                                             ACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGG                                                        <  SRR19973068.731498/150‑1 (MQ=60)
                                                                                              CGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGC                                                       <  SRR19973068.3033721/150‑1 (MQ=60)
                                                                                                  GCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTG                                                   >  SRR19973068.466416/1‑150 (MQ=60)
                                                                                                   CATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGA                                                  <  SRR19973068.3104125/150‑1 (MQ=60)
                                                                                                       TTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTT                                              <  SRR19973068.3206029/150‑1 (MQ=60)
                                                                                                        TGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTA                                             >  SRR19973068.214424/1‑150 (MQ=60)
                                                                                                        TGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTA                                             <  SRR19973068.2167002/150‑1 (MQ=60)
                                                                                                        TGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTA                                             <  SRR19973068.2978765/150‑1 (MQ=60)
                                                                                                        TGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTA                                             <  SRR19973068.3747278/150‑1 (MQ=60)
                                                                                                        TGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTA                                             <  SRR19973068.3747163/150‑1 (MQ=60)
                                                                                                          TCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATT                                           <  SRR19973068.2074462/150‑1 (MQ=60)
                                                                                                                CCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTAT                                     <  SRR19973068.3798262/150‑1 (MQ=60)
                                                                                                                CCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTAT                                     <  SRR19973068.3171841/150‑1 (MQ=60)
                                                                                                                   GATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGAC                                  >  SRR19973068.50647/1‑150 (MQ=60)
                                                                                                                     TACGCTGCATTGCACTTACGAACAATATTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGTTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGA                                <  SRR19973068.643342/150‑1 (MQ=60)
                                                                                                                       CGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATC                              >  SRR19973068.3654570/1‑150 (MQ=60)
                                                                                                                        GCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCG                             <  SRR19973068.2934971/150‑1 (MQ=60)
                                                                                                                        GCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCG                             <  SRR19973068.863422/150‑1 (MQ=60)
                                                                                                                        GCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCG                             <  SRR19973068.3627275/150‑1 (MQ=60)
                                                                                                                        GCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCG                             <  SRR19973068.3627349/150‑1 (MQ=60)
                                                                                                                        GCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCG                             <  SRR19973068.1685687/150‑1 (MQ=60)
                                                                                                                        GCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCG                             <  SRR19973068.1568644/150‑1 (MQ=60)
                                                                                                                          TGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAATGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCC                           >  SRR19973068.1021262/1‑150 (MQ=60)
                                                                                                                              TTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTG                       <  SRR19973068.234830/150‑1 (MQ=60)
                                                                                                                              TTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTG                       <  SRR19973068.2321576/150‑1 (MQ=60)
                                                                                                                                 CAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAG                    <  SRR19973068.1467153/150‑1 (MQ=60)
                                                                                                                                   GTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCG                  >  SRR19973068.645586/1‑150 (MQ=60)
                                                                                                                                    TTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGA                 >  SRR19973068.2006421/1‑150 (MQ=60)
                                                                                                                                    TTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGA                 >  SRR19973068.778777/1‑150 (MQ=60)
                                                                                                                                     TACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAA                >  SRR19973068.521515/1‑150 (MQ=60)
                                                                                                                                       CGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAA              >  SRR19973068.3437640/1‑150 (MQ=60)
                                                                                                                                       CGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAA              >  SRR19973068.1376770/1‑150 (MQ=60)
                                                                                                                                       CGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAA              >  SRR19973068.2841299/1‑150 (MQ=60)
                                                                                                                                           GAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAG          <  SRR19973068.2474688/150‑1 (MQ=60)
                                                                                                                                           GAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAG          <  SRR19973068.2474811/150‑1 (MQ=60)
                                                                                                                                             ATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTT        >  SRR19973068.1527038/1‑150 (MQ=60)
                                                                                                                                             ATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTT        <  SRR19973068.3330631/150‑1 (MQ=60)
                                                                                                                                              TTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGTATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTC       <  SRR19973068.2165596/150‑1 (MQ=60)
                                                                                                                                              TTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTC       <  SRR19973068.74586/150‑1 (MQ=60)
                                                                                                                                                TTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAA     <  SRR19973068.740715/150‑1 (MQ=60)
                                                                                                                                                 TGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAAT    >  SRR19973068.1256345/1‑150 (MQ=60)
                                                                                                                                                   AGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCG  >  SRR19973068.4124175/1‑150 (MQ=60)
                                                                                                                                                   AGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCG  >  SRR19973068.2076784/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
AGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCG  >  CP009273/2847855‑2848151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑