Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,798,582 C→A 20.6% Q243H (CAG→CAT)  waaP ← kinase that phosphorylates core heptose of lipopolysaccharide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,798,5820CA20.6% 979.3 / 175.0 381Q243H (CAG→CAT) waaPkinase that phosphorylates core heptose of lipopolysaccharide
Reads supporting (aligned to +/- strand):  ref base C (167/135);  new base A (45/34);  total (212/169)
Fisher's exact test for biased strand distribution p-value = 8.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

Only 100 of 387 total aligned reads displayed.

TTTAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCACG  >  CP009273/3798433‑3798731
                                                                                                                                                     |                                                                                                                                                     
tttAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTc                                                                                                                                                       <  1:5013773/150‑1 (MQ=255)
   aaTTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGt                                                                                                                                                    >  1:5051918/1‑150 (MQ=255)
      tCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCa                                                                                                                                                 >  2:205089/1‑150 (MQ=255)
      tCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCa                                                                                                                                                 <  1:2186304/150‑1 (MQ=255)
       cATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCaa                                                                                                                                                >  1:1603591/1‑150 (MQ=255)
          aaTCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTATGTTCAagag                                                                                                                                             >  1:4255837/1‑150 (MQ=255)
           aTCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAgaga                                                                                                                                            >  1:3476703/1‑150 (MQ=255)
           aTCTGCAACCAAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAgaga                                                                                                                                            <  2:4358526/150‑1 (MQ=255)
               gCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAat                                                                                                                                        >  2:2415559/1‑150 (MQ=255)
               gCAAACCAATTTATAAAATAAAGAGTCTTATTAAAGTTAGTTCCAGTTCATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTTACGCTTGCCATGTTCAATAAATTCTGTTCATGAGACAat                                                                                                                                        <  2:2281385/150‑1 (MQ=255)
                 aaCCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTTCCATGTTCAATAAATTATGTTCAAGAGACAatat                                                                                                                                      >  2:2501582/1‑150 (MQ=255)
                 aaCACAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAatat                                                                                                                                      <  2:1499085/150‑1 (MQ=255)
                 aaCACAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAatat                                                                                                                                      <  2:5135352/150‑1 (MQ=255)
                  aCCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATAtt                                                                                                                                     >  2:1142062/1‑150 (MQ=255)
                    acAATTTATAAAATAAATAGTAATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATAtttt                                                                                                                                   <  2:2996760/149‑1 (MQ=255)
                     cAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTg                                                                                                                                  >  2:4917448/1‑150 (MQ=255)
                     cAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTg                                                                                                                                  >  2:4275360/1‑150 (MQ=255)
                           ataaaataaaTAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTaaa                                                                                                                            >  1:4386813/1‑150 (MQ=255)
                            taaaataaaTAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAg                                                                                                                           <  2:1705399/150‑1 (MQ=255)
                            taaaataaaTAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAg                                                                                                                           >  1:3345063/1‑150 (MQ=255)
                              aaataaatAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGc                                                                                                                         >  1:3873035/1‑150 (MQ=255)
                               aataaataGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCa                                                                                                                        <  2:3874337/150‑1 (MQ=255)
                                ataaataGTCATAATAAAGTTAGTTCCAGTACATACTAATAACTATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCAt                                                                                                                       <  1:250942/150‑1 (MQ=255)
                                ataaataGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCAt                                                                                                                       <  2:3713121/150‑1 (MQ=255)
                                  aaataGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTTCGTAAAGGCATcc                                                                                                                     >  2:3386606/1‑150 (MQ=255)
                                        tCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAAt                                                                                                               >  1:5084230/1‑150 (MQ=255)
                                          ataataAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATaa                                                                                                             >  2:63098/1‑150 (MQ=255)
                                          ataataAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATaa                                                                                                             >  2:1090914/1‑150 (MQ=255)
                                             ataaAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAAcc                                                                                                          >  2:2559522/1‑150 (MQ=255)
                                             ataaAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAAcc                                                                                                          <  2:3081933/150‑1 (MQ=255)
                                                   ttagttCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATa                                                                                                    <  1:3760507/150‑1 (MQ=255)
                                                   ttagttCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATa                                                                                                    <  1:4515655/150‑1 (MQ=255)
                                                     agttCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATaaa                                                                                                  >  1:4527385/1‑150 (MQ=255)
                                                       ttCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATc                                                                                                <  1:2065394/150‑1 (MQ=255)
                                                        tCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCg                                                                                               <  1:845338/150‑1 (MQ=255)
                                                              aCATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGat                                                                                         >  2:2756714/1‑150 (MQ=255)
                                                                aTACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGatat                                                                                       >  2:5069252/1‑150 (MQ=255)
                                                                 tACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATAtc                                                                                      >  2:1346301/1‑150 (MQ=255)
                                                                  aCTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATAtct                                                                                     >  1:3988271/1‑150 (MQ=255)
                                                                   cTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATAtctc                                                                                    >  1:1012531/1‑150 (MQ=255)
                                                                     aataaataTTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGTCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCtt                                                                                  <  1:513101/150‑1 (MQ=255)
                                                                     aataaataTTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCtt                                                                                  >  1:952558/1‑150 (MQ=255)
                                                                      ataaataTTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCttt                                                                                 >  1:2965191/1‑150 (MQ=255)
                                                                      ataaataTTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCttt                                                                                 <  2:3994237/150‑1 (MQ=255)
                                                                      ataaataTTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCttt                                                                                 >  1:4873348/1‑150 (MQ=255)
                                                                       taaataTTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTg                                                                                <  1:4023172/150‑1 (MQ=255)
                                                                       taaataTTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTACATAAATCGCCAGATATCTCTTTg                                                                                >  1:2772000/1‑150 (MQ=255)
                                                                        aaataTTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGc                                                                               >  1:3299062/1‑150 (MQ=255)
                                                                        aaataTTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGc                                                                               <  2:1885914/150‑1 (MQ=255)
                                                                            atTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCa                                                                           <  2:3654559/150‑1 (MQ=255)
                                                                              ttttATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAgg                                                                         <  1:2843137/150‑1 (MQ=255)
                                                                                ttATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGcc                                                                       <  1:1848677/150‑1 (MQ=255)
                                                                                 tataATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCa                                                                      >  2:604045/1‑150 (MQ=255)
                                                                                   taATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTTAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAat                                                                    >  2:4147721/1‑150 (MQ=255)
                                                                                   taATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAat                                                                    <  1:1931020/150‑1 (MQ=255)
                                                                                   taATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAat                                                                    >  1:1810830/1‑150 (MQ=255)
                                                                                    aaTCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAata                                                                   >  2:354284/1‑150 (MQ=255)
                                                                                     aTCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAatat                                                                  <  1:2948638/150‑1 (MQ=255)
                                                                                      tCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATAtt                                                                 <  2:1445278/150‑1 (MQ=255)
                                                                                      tCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTACAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATAtt                                                                 <  1:454672/150‑1 (MQ=255)
                                                                                       ccTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTc                                                                >  2:1577586/1‑150 (MQ=255)
                                                                                          ttGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCAtt                                                             <  1:1017548/150‑1 (MQ=255)
                                                                                           ttcGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTAGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCCAAATAAACCTGCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAAAATTCATtg                                                            >  2:3462993/3‑150 (MQ=255)
                                                                                           tGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATtg                                                            >  2:905091/1‑150 (MQ=255)
                                                                                             cGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCTAATATTCATtgat                                                          <  2:4535192/150‑1 (MQ=255)
                                                                                             cGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATtgat                                                          <  1:4701468/150‑1 (MQ=255)
                                                                                              gTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATtgatg                                                         >  2:2697401/1‑150 (MQ=255)
                                                                                              gTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATtgatg                                                         >  1:1341748/1‑150 (MQ=255)
                                                                                                 gtgtTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATgaga                                                      >  1:2309223/1‑150 (MQ=255)
                                                                                                  tgtTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGaa                                                     >  1:537303/1‑150 (MQ=255)
                                                                                                     tCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAata                                                  >  1:3651847/1‑150 (MQ=255)
                                                                                                      cGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAatat                                                 <  1:741398/150‑1 (MQ=255)
                                                                                                          ctTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAAc                                             <  1:2593774/150‑1 (MQ=255)
                                                                                                              aaTACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAAt                                         <  1:169366/150‑1 (MQ=255)
                                                                                                              aaTACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAAt                                         <  2:805336/150‑1 (MQ=255)
                                                                                                               aTACGCTCGGCCTTAACACTTGCCATGTTCAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATc                                        <  1:3180701/150‑1 (MQ=255)
                                                                                                                  cGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAgg                                     <  2:2823816/150‑1 (MQ=255)
                                                                                                                    cTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTc                                   >  2:5011305/1‑150 (MQ=255)
                                                                                                                          cTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATc                             <  1:1273453/150‑1 (MQ=255)
                                                                                                                          cTTAACGCTTGCCATGTTCAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCATGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATc                             <  2:1248795/150‑1 (MQ=255)
                                                                                                                           ttAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATcg                            <  2:3164698/150‑1 (MQ=255)
                                                                                                                           ttAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATcg                            <  1:1197722/150‑1 (MQ=255)
                                                                                                                               cGCTTGCCATGTTCAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGcc                        >  2:4079824/1‑150 (MQ=255)
                                                                                                                                 cTTGCCATGTTCAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAg                      >  1:2003111/1‑150 (MQ=255)
                                                                                                                                   tGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAgcg                    >  2:286746/1‑150 (MQ=255)
                                                                                                                                   tGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAgcg                    >  2:4737863/1‑150 (MQ=255)
                                                                                                                                     ccATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAgcggc                  >  1:2868333/1‑150 (MQ=255)
                                                                                                                                      cATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAgcggcg                 <  2:3326955/150‑1 (MQ=255)
                                                                                                                                       aTGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGgcgc                >  1:3415680/1‑150 (MQ=255)
                                                                                                                                            cAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTAc           >  1:3137130/1‑150 (MQ=255)
                                                                                                                                            cAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTAc           >  1:155815/1‑150 (MQ=255)
                                                                                                                                            cAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTAc           >  2:2216888/1‑150 (MQ=255)
                                                                                                                                            cAATAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTAc           <  1:2136050/150‑1 (MQ=255)
                                                                                                                                             aaTAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCTCGCCAGCGGCGCGGTACt          >  1:2707962/1‑150 (MQ=255)
                                                                                                                                             aaTAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATAGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACt          >  2:2067931/1‑150 (MQ=255)
                                                                                                                                             aaTAAATTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACt          >  1:2495394/1‑150 (MQ=255)
                                                                                                                                                 aaTTATGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTg      <  2:3805542/150‑1 (MQ=255)
                                                                                                                                                  atacTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGc     <  2:4448878/147‑1 (MQ=255)
                                                                                                                                                    tCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCAc   >  1:4070854/1‑150 (MQ=255)
                                                                                                                                                     cTGTACAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCACg  <  1:4078390/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCACG  >  CP009273/3798433‑3798731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A