Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,163,117 T→A 69.7% I36I (ATT→ATA)  birA → bifunctional biotin‑[acetylCoA carboxylase] holoenzyme synthetase/ DNA‑binding transcriptional repressor, bio‑5'‑AMP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,163,1170TA69.7% 416.0 / 231.3 276I36I (ATT→ATA) birAbifunctional biotin‑[acetylCoA carboxylase] holoenzyme synthetase/ DNA‑binding transcriptional repressor, bio‑5'‑AMP‑binding
Reads supporting (aligned to +/- strand):  ref base T (30/52);  new base A (83/109);  total (113/163)
Fisher's exact test for biased strand distribution p-value = 3.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 288 total aligned reads displayed.

TATTGGTGCATCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAG  >  CP009273/4162968‑4163265
                                                                                                                                                     |                                                                                                                                                    
 aTTGGTGCATCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATta                                                                                                                                                     <  2:3243081/150‑1 (MQ=255)
 aTTGGTGCATCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATta                                                                                                                                                     <  1:1530701/150‑1 (MQ=255)
       gCATCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAac                                                                                                                                               <  1:4415879/150‑1 (MQ=255)
       gCATCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAac                                                                                                                                               >  2:4347755/1‑150 (MQ=255)
        cATCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAaca                                                                                                                                              >  1:5004384/1‑150 (MQ=255)
          tCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAAcaca                                                                                                                                            <  1:4076368/150‑1 (MQ=255)
          tCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAAcaca                                                                                                                                            >  1:1182122/1‑150 (MQ=255)
           cAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAAGTGATGGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGTGCAGTTGGGTGCAACGCTGGGAATGTGCCGGGCGGCTATAATTAAacacct                                                                                                                                           >  2:984076/1‑148 (MQ=255)
            aGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAACTTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTTATAAACACAtt                                                                                                                                          <  1:1728710/150‑1 (MQ=255)
                   gTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGacac                                                                                                                                   <  1:5314725/150‑1 (MQ=255)
                        cgcAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGt                                                                                                                              <  1:733938/150‑1 (MQ=255)
                           aGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGAc                                                                                                                           >  1:3819416/1‑150 (MQ=255)
                           aGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGAc                                                                                                                           <  2:2199554/150‑1 (MQ=255)
                           aGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGAc                                                                                                                           <  2:4195755/150‑1 (MQ=255)
                             tGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTg                                                                                                                         <  2:1050202/150‑1 (MQ=255)
                             tGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTg                                                                                                                         <  1:1444837/150‑1 (MQ=255)
                                 gaCAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGc                                                                                                                     <  2:4844152/150‑1 (MQ=255)
                                   cAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGt                                                                                                                   >  2:5298706/1‑150 (MQ=255)
                                    aaTTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGtt                                                                                                                  >  2:3605106/1‑150 (MQ=255)
                                     aTTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTg                                                                                                                 <  1:2116224/150‑1 (MQ=255)
                                       ttCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGAt                                                                                                               <  2:2666625/150‑1 (MQ=255)
                                       ttCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGAt                                                                                                               <  2:2320223/150‑1 (MQ=255)
                                         cATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGt                                                                                                             >  2:2435351/1‑150 (MQ=255)
                                          aTGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTc                                                                                                            >  1:5135263/1‑150 (MQ=255)
                                          aTGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTc                                                                                                            >  1:516164/1‑150 (MQ=255)
                                           tGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCt                                                                                                           >  1:2036625/1‑150 (MQ=255)
                                            gAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCtt                                                                                                          >  1:2056604/1‑150 (MQ=255)
                                                gATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTAcc                                                                                                      <  2:3447380/150‑1 (MQ=255)
                                                        cGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCggg                                                                                              >  2:1571173/1‑150 (MQ=255)
                                                           gCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTaa                                                                                           >  2:3420661/1‑150 (MQ=255)
                                                           gCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTaa                                                                                           >  1:3252518/1‑150 (MQ=255)
                                                                tGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGAt                                                                                      <  2:4769012/150‑1 (MQ=255)
                                                                tGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGAt                                                                                      >  1:2106406/1‑150 (MQ=255)
                                                                tGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGAt                                                                                      >  1:3435549/1‑150 (MQ=255)
                                                                tGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGAt                                                                                      <  1:3170930/150‑1 (MQ=255)
                                                                tGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGAt                                                                                      <  2:1069914/150‑1 (MQ=255)
                                                                tGAAATTGATTGCCCTGTTAGCGAACGGTGAATGTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGAt                                                                                      <  2:3343200/150‑1 (MQ=255)
                                                                 gAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATa                                                                                     <  1:4499957/150‑1 (MQ=255)
                                                                      tgattgCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAgcc                                                                                <  1:2696704/150‑1 (MQ=255)
                                                                        attgCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAgcctg                                                                              >  1:1946709/1‑150 (MQ=255)
                                                                          tgCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAgcctgcc                                                                            >  2:4270907/1‑150 (MQ=255)
                                                                           gCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAgcctgcct                                                                           <  1:1221607/150‑1 (MQ=255)
                                                                            cccTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGcctgcctg                                                                          >  1:4639954/1‑150 (MQ=255)
                                                                             ccTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGa                                                                         <  2:1868331/150‑1 (MQ=255)
                                                                             ccTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGa                                                                         >  1:1697247/1‑150 (MQ=255)
                                                                                  tAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTa                                                                    <  1:2322739/150‑1 (MQ=255)
                                                                                  tAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTa                                                                    >  2:581052/1‑150 (MQ=255)
                                                                                   aGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTAt                                                                   <  2:3118762/150‑1 (MQ=255)
                                                                                      gAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCa                                                                <  2:5257430/150‑1 (MQ=255)
                                                                                       aaCGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAg                                                               <  2:1323811/150‑1 (MQ=255)
                                                                                        aCGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGt                                                              <  2:4030760/150‑1 (MQ=255)
                                                                                         cGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATATAGCCTGCCTGAGCCTATCCAGtt                                                             <  1:1853002/150‑1 (MQ=255)
                                                                                           gTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTAc                                                           <  1:1512385/150‑1 (MQ=255)
                                                                                            tGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACt                                                          <  1:3319748/150‑1 (MQ=255)
                                                                                              aaTTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTa                                                        >  2:3653432/1‑150 (MQ=255)
                                                                                              aaTTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTa                                                        >  2:4138989/1‑150 (MQ=255)
                                                                                                tttCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAAt                                                      <  2:2946083/150‑1 (MQ=255)
                                                                                                tttCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAAt                                                      <  1:892567/150‑1 (MQ=255)
                                                                                                  tCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGc                                                    >  2:1258968/1‑150 (MQ=255)
                                                                                                   cACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCt                                                   <  2:482557/150‑1 (MQ=255)
                                                                                                   cACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCt                                                   <  2:81697/150‑1 (MQ=255)
                                                                                                   cACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCt                                                   <  1:1335739/150‑1 (MQ=255)
                                                                                                     ctctGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTaa                                                 >  1:4196516/1‑150 (MQ=255)
                                                                                                      tctGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTaaa                                                <  2:3875667/150‑1 (MQ=255)
                                                                                                       ctGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAAc                                               <  1:2610456/150‑1 (MQ=255)
                                                                                                        tGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACa                                              >  2:1140268/1‑150 (MQ=255)
                                                                                                           cGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGat                                           <  2:4538261/150‑1 (MQ=255)
                                                                                                           cGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGat                                           <  1:1070684/150‑1 (MQ=255)
                                                                                                             aGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGatat                                         <  2:5004384/150‑1 (MQ=255)
                                                                                                              gCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATAtt                                        <  2:2385264/150‑1 (MQ=255)
                                                                                                               cAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTg                                       <  2:2106943/150‑1 (MQ=255)
                                                                                                               cAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTg                                       <  1:1956289/150‑1 (MQ=255)
                                                                                                               cAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTg                                       <  1:3005502/150‑1 (MQ=255)
                                                                                                                aGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTgg                                      >  2:952686/1‑150 (MQ=255)
                                                                                                                aGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTgg                                      >  1:2426871/1‑150 (MQ=255)
                                                                                                                 gTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTggg                                     >  2:4254594/1‑150 (MQ=255)
                                                                                                                   tGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTc                                   <  2:1958004/150‑1 (MQ=255)
                                                                                                                   tGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTc                                   <  1:2873767/150‑1 (MQ=255)
                                                                                                                      gTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGc                                <  1:4050833/150‑1 (MQ=255)
                                                                                                                      gTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGc                                <  1:3515633/150‑1 (MQ=255)
                                                                                                                      gTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGc                                <  2:3313950/150‑1 (MQ=255)
                                                                                                                       tGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTGCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGTGAGTGGGTCAGCt                               >  2:2918853/1‑150 (MQ=255)
                                                                                                                       tGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCt                               <  2:4382087/150‑1 (MQ=255)
                                                                                                                        gAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGAGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTg                              >  1:4817113/1‑150 (MQ=255)
                                                                                                                          aaCGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGa                            <  2:1099809/150‑1 (MQ=255)
                                                                                                                          aaCGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGa                            >  1:2171587/1‑150 (MQ=255)
                                                                                                                           aCGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGAt                           <  1:975314/150‑1 (MQ=255)
                                                                                                                               tGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCCGCTGGATGGCg                       >  2:2586301/1‑150 (MQ=255)
                                                                                                                               tGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCg                       >  2:5026966/1‑150 (MQ=255)
                                                                                                                                  gAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTa                    >  1:3861168/1‑150 (MQ=255)
                                                                                                                                    aTGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAgt                  >  2:2069779/1‑150 (MQ=255)
                                                                                                                                     tGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAgtg                 >  2:1747856/1‑150 (MQ=255)
                                                                                                                                     tGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAgtg                 <  1:1562487/150‑1 (MQ=255)
                                                                                                                                      gAGCCGGGCGGCTATTAATAAACACATTCAGACACTGAGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAgtgt                <  2:1614781/150‑1 (MQ=255)
                                                                                                                                      gAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAgtgt                <  1:3689034/150‑1 (MQ=255)
                                                                                                                                             gcggcTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGtg         <  1:278080/150‑1 (MQ=255)
                                                                                                                                               ggcTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGtgct       >  2:1466888/1‑150 (MQ=255)
                                                                                                                                                 cTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGtgctgc     >  2:4314990/1‑150 (MQ=255)
                                                                                                                                                 cTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGtgctgc     >  1:3128303/1‑150 (MQ=255)
                                                                                                                                                  tataAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGcc    >  2:1653999/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TATTGGTGCATCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAG  >  CP009273/4162968‑4163265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATTGGTGCATCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAG  >  CP009273/4162968‑4163265
                                                                                                                                                     |                                                                                                                                                    
TATTGGTGCATCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGACTGAGCCGGGCGGCTATT                                                                                                                                                      >  SRR19973062.4477365/1‑150 (MQ=60)
TATTGGTGCATCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATT                                                                                                                                                      >  SRR19973062.2117687/1‑150 (MQ=60)
TATTGGTGCATCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATT                                                                                                                                                      >  SRR19973062.2118282/1‑150 (MQ=60)
 ATTGGTGCATCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTA                                                                                                                                                     <  SRR19973062.1538245/150‑1 (MQ=60)
 ATTGGTGCATCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTA                                                                                                                                                     <  SRR19973062.1538246/150‑1 (MQ=60)
 ATTGGTGCATCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAA                                                                                                                                                     <  SRR19973062.951073/150‑1 (MQ=60)
       GCATCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAAC                                                                                                                                               <  SRR19973062.4438452/150‑1 (MQ=60)
        CATCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACA                                                                                                                                              >  SRR19973062.5030856/1‑150 (MQ=60)
         ATCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACAC                                                                                                                                             <  SRR19973062.5152353/150‑1 (MQ=60)
          TCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACA                                                                                                                                            <  SRR19973062.4097321/150‑1 (MQ=60)
          TCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACA                                                                                                                                            <  SRR19973062.2463629/150‑1 (MQ=60)
          TCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACA                                                                                                                                            >  SRR19973062.3870917/1‑150 (MQ=60)
          TCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACA                                                                                                                                            >  SRR19973062.1187650/1‑150 (MQ=60)
            AGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAACTTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTTATAAACACATT                                                                                                                                          <  SRR19973062.1737131/150‑1 (MQ=60)
            AGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGACTTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGACTGAGCCGGGCGGCTCTTCATAAACACATT                                                                                                                                          >  SRR19973062.683835/1‑150 (MQ=60)
            AGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATT                                                                                                                                          >  SRR19973062.82241/1‑150 (MQ=60)
            AGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATT                                                                                                                                          <  SRR19973062.1118526/150‑1 (MQ=60)
            AGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATT                                                                                                                                          <  SRR19973062.965605/150‑1 (MQ=60)
              GTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCA                                                                                                                                        >  SRR19973062.1214453/1‑150 (MQ=60)
               TGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAG                                                                                                                                       >  SRR19973062.2813091/1‑150 (MQ=60)
                 AAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGAC                                                                                                                                     <  SRR19973062.3574152/150‑1 (MQ=60)
                 AAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGAC                                                                                                                                     >  SRR19973062.3520133/1‑150 (MQ=60)
                   GTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACAC                                                                                                                                   <  SRR19973062.5342621/150‑1 (MQ=60)
                    TGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACT                                                                                                                                  <  SRR19973062.1849448/150‑1 (MQ=60)
                    TGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACT                                                                                                                                  <  SRR19973062.2734675/150‑1 (MQ=60)
                     GAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTG                                                                                                                                 <  SRR19973062.1234849/150‑1 (MQ=60)
                      AGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGC                                                                                                                                <  SRR19973062.79258/150‑1 (MQ=60)
                       GCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATGTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCG                                                                                                                               <  SRR19973062.243581/150‑1 (MQ=60)
                        CGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGT                                                                                                                              <  SRR19973062.737271/150‑1 (MQ=60)
                           AGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGAC                                                                                                                           >  SRR19973062.3838949/1‑150 (MQ=60)
                           AGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGAC                                                                                                                           <  SRR19973062.1299320/150‑1 (MQ=60)
                             TGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTG                                                                                                                         <  SRR19973062.1451867/150‑1 (MQ=60)
                              GGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGG                                                                                                                        <  SRR19973062.4515846/150‑1 (MQ=60)
                               GAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGG                                                                                                                       >  SRR19973062.3291296/1‑150 (MQ=60)
                                 GACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGC                                                                                                                     >  SRR19973062.3331085/1‑150 (MQ=60)
                                   CAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGACCGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTCTTAATAAACACATTCAGACACTGCGTGACTGGGGCGT                                                                                                                   >  SRR19973062.4066735/1‑150 (MQ=60)
                                    AATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTT                                                                                                                  <  SRR19973062.1598432/150‑1 (MQ=60)
                                     ATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTG                                                                                                                 <  SRR19973062.2126991/150‑1 (MQ=60)
                                          ATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTC                                                                                                            >  SRR19973062.5162424/1‑150 (MQ=60)
                                          ATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTC                                                                                                            >  SRR19973062.518587/1‑150 (MQ=60)
                                           TGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCT                                                                                                           >  SRR19973062.2047082/1‑150 (MQ=60)
                                            GAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTT                                                                                                          >  SRR19973062.2067132/1‑150 (MQ=60)
                                            GAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTT                                                                                                          >  SRR19973062.2066954/1‑150 (MQ=60)
                                            GAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTT                                                                                                          <  SRR19973062.5178355/150‑1 (MQ=60)
                                               ggctcACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTAC                                                                                                       <  SRR19973062.4866095/145‑1 (MQ=60)
                                               GGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAATCGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTAC                                                                                                       <  SRR19973062.71644/150‑1 (MQ=60)
                                                GATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACC                                                                                                      <  SRR19973062.3255139/150‑1 (MQ=60)
                                                       CCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGG                                                                                               <  SRR19973062.2057580/150‑1 (MQ=60)
                                                        CGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGG                                                                                              >  SRR19973062.3481794/1‑150 (MQ=60)
                                                           GCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAA                                                                                           >  SRR19973062.3269393/1‑150 (MQ=60)
                                                             CACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAG                                                                                         <  SRR19973062.973420/150‑1 (MQ=60)
                                                             CACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAG                                                                                         <  SRR19973062.1468908/150‑1 (MQ=60)
                                                             CACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAAAAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTAACGTTACGGGTAAAG                                                                                         >  SRR19973062.4086866/1‑150 (MQ=60)
                                                               CTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGA                                                                                       >  SRR19973062.2975441/1‑150 (MQ=60)
                                                                TGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGAT                                                                                      >  SRR19973062.2117147/1‑150 (MQ=60)
                                                                TGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGAT                                                                                      >  SRR19973062.3453079/1‑150 (MQ=60)
                                                                TGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGAT                                                                                      >  SRR19973062.3453106/1‑150 (MQ=60)
                                                                TGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGAT                                                                                      <  SRR19973062.3187368/150‑1 (MQ=60)
                                                                 GAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATA                                                                                     <  SRR19973062.4523229/150‑1 (MQ=60)
                                                                   AATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACA                                                                                   <  SRR19973062.5278923/150‑1 (MQ=60)
                                                                    ATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAG                                                                                  >  SRR19973062.4586708/1‑150 (MQ=60)
                                                                     TTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGC                                                                                 >  SRR19973062.2765384/1‑150 (MQ=60)
                                                                      TGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCC                                                                                <  SRR19973062.2710833/150‑1 (MQ=60)
                                                                       GATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCT                                                                               >  SRR19973062.1957060/1‑150 (MQ=60)
                                                                       GATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCT                                                                               <  SRR19973062.1705790/150‑1 (MQ=60)
                                                                        ATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCTGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTG                                                                              <  SRR19973062.2930806/150‑1 (MQ=60)
                                                                        ATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTG                                                                              >  SRR19973062.1956706/1‑150 (MQ=60)
                                                                        ATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTG                                                                              >  SRR19973062.1956713/1‑150 (MQ=60)
                                                                        ATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGATGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCAGTGTAAAGGATACAGCCTG                                                                              >  SRR19973062.1956636/1‑150 (MQ=60)
                                                                          TGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCC                                                                            <  SRR19973062.2672262/150‑1 (MQ=60)
                                                                          TGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCC                                                                            <  SRR19973062.2672283/150‑1 (MQ=60)
                                                                           GCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCT                                                                           <  SRR19973062.1227286/150‑1 (MQ=60)
                                                                            CCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTG                                                                          >  SRR19973062.4664232/1‑150 (MQ=60)
                                                                             CCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGA                                                                         >  SRR19973062.1705566/1‑150 (MQ=60)
                                                                              CTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAG                                                                        <  SRR19973062.3202156/150‑1 (MQ=60)
                                                                              CTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAG                                                                        >  SRR19973062.4775412/1‑150 (MQ=60)
                                                                               TGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGC                                                                       >  SRR19973062.4086773/1‑150 (MQ=60)
                                                                                GTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCC                                                                      >  SRR19973062.4200705/1‑150 (MQ=60)
                                                                                  TAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTA                                                                    <  SRR19973062.2335009/150‑1 (MQ=60)
                                                                                   AGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTAT                                                                   <  SRR19973062.4203030/150‑1 (MQ=60)
                                                                                   AGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTAT                                                                   <  SRR19973062.3820828/150‑1 (MQ=60)
                                                                                         CGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATATAGCCTGCCTGAGCCTATCCAGTT                                                             <  SRR19973062.5002574/150‑1 (MQ=60)
                                                                                         CGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATATAGCCTGCCTGAGCCTATCCAGTT                                                             <  SRR19973062.1862275/150‑1 (MQ=60)
                                                                                          GGTGAAGTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTA                                                            <  SRR19973062.3859887/150‑1 (MQ=60)
                                                                                           GTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTAC                                                           <  SRR19973062.1519838/150‑1 (MQ=60)
                                                                                           GTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTAC                                                           <  SRR19973062.1520081/150‑1 (MQ=60)
                                                                                            TGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACT                                                          <  SRR19973062.3336902/150‑1 (MQ=60)
                                                                                             GAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTT                                                         <  SRR19973062.1588627/150‑1 (MQ=60)
                                                                                             GAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTT                                                         >  SRR19973062.2136495/1‑150 (MQ=60)
                                                                                               ATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAA                                                       >  SRR19973062.3045889/1‑150 (MQ=60)
                                                                                                TTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAAT                                                      <  SRR19973062.896382/150‑1 (MQ=60)
                                                                                                TTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAAT                                                      <  SRR19973062.1426751/150‑1 (MQ=60)
                                                                                                   CACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCT                                                   >  SRR19973062.3056316/1‑150 (MQ=60)
                                                                                                   CACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCT                                                   <  SRR19973062.5171291/150‑1 (MQ=60)
                                                                                                   CACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCT                                                   <  SRR19973062.1341796/150‑1 (MQ=60)
                                                                                                    ACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTA                                                  >  SRR19973062.1223587/1‑150 (MQ=60)
                                                                                                     CTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGATCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAA                                                 >  SRR19973062.3432726/1‑150 (MQ=60)
                                                                                                     CTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAA                                                 >  SRR19973062.4218307/1‑150 (MQ=60)
                                                                                                     CTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAA                                                 >  SRR19973062.4218283/1‑150 (MQ=60)
                                                                                                       CTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAAC                                               <  SRR19973062.2624245/150‑1 (MQ=60)
                                                                                                        TGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACA                                              <  SRR19973062.5290051/150‑1 (MQ=60)
                                                                                                          GCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGA                                            <  SRR19973062.3593387/150‑1 (MQ=60)
                                                                                                           CGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGAT                                           <  SRR19973062.1075729/150‑1 (MQ=60)
                                                                                                             AGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATAT                                         <  SRR19973062.318480/150‑1 (MQ=60)
                                                                                                               CAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTG                                       <  SRR19973062.3021211/150‑1 (MQ=60)
                                                                                                               CAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTG                                       <  SRR19973062.1966350/150‑1 (MQ=60)
                                                                                                                AGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGG                                      >  SRR19973062.2439898/1‑150 (MQ=60)
                                                                                                                   TGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTC                                   >  SRR19973062.2090639/1‑150 (MQ=60)
                                                                                                                   TGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTC                                   <  SRR19973062.2888961/150‑1 (MQ=60)
                                                                                                                   TGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTC                                   >  SRR19973062.2860672/1‑150 (MQ=60)
                                                                                                                    GGGTGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCA                                  >  SRR19973062.4605571/1‑150 (MQ=60)
                                                                                                                      GTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGC                                <  SRR19973062.4071551/150‑1 (MQ=60)
                                                                                                                      GTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGC                                <  SRR19973062.3533439/150‑1 (MQ=60)
                                                                                                                       TGAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCT                               >  SRR19973062.2000134/1‑150 (MQ=60)
                                                                                                                        TAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTG                              <  SRR19973062.2327065/150‑1 (MQ=60)
                                                                                                                        GAAACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGAGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTG                              >  SRR19973062.4842061/1‑150 (MQ=60)
                                                                                                                          AACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGA                            >  SRR19973062.2182544/1‑150 (MQ=60)
                                                                                                                          AACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGA                            >  SRR19973062.182305/1‑150 (MQ=60)
                                                                                                                           ACGCTGGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGAT                           <  SRR19973062.979824/150‑1 (MQ=60)
                                                                                                                             GCTGGGAATGAGCCGGGCGGCTGTAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGG                         <  SRR19973062.2541341/150‑1 (MQ=60)
                                                                                                                                GGGAATGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGG                      <  SRR19973062.1468205/150‑1 (MQ=60)
                                                                                                                                  GAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTA                    >  SRR19973062.3880879/1‑150 (MQ=60)
                                                                                                                                     TGAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTG                 <  SRR19973062.1570205/150‑1 (MQ=60)
                                                                                                                                      GAGCCGGGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGT                <  SRR19973062.3707963/150‑1 (MQ=60)
                                                                                                                                        GCCGGGCTGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAG              <  SRR19973062.1924006/150‑1 (MQ=60)
                                                                                                                                          CGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCC            <  SRR19973062.2284364/150‑1 (MQ=60)
                                                                                                                                            GGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGT          <  SRR19973062.1563287/150‑1 (MQ=60)
                                                                                                                                            GGCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGT          <  SRR19973062.1563372/150‑1 (MQ=60)
                                                                                                                                             GCGGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTG         <  SRR19973062.279448/150‑1 (MQ=60)
                                                                                                                                              CGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGC        <  SRR19973062.2042473/150‑1 (MQ=60)
                                                                                                                                               GGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCT       <  SRR19973062.3757702/150‑1 (MQ=60)
                                                                                                                                               GGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCT       <  SRR19973062.4819722/150‑1 (MQ=60)
                                                                                                                                               GGCTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCT       >  SRR19973062.2346782/1‑150 (MQ=60)
                                                                                                                                                 CTATAAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGC     >  SRR19973062.3144401/1‑150 (MQ=60)
                                                                                                                                                  TATAAAGAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCC    <  SRR19973062.573386/150‑1 (MQ=60)
                                                                                                                                                   ATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCA   >  SRR19973062.1419672/1‑150 (MQ=60)
                                                                                                                                                    TTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAG  >  SRR19973062.2078934/1‑150 (MQ=60)
                                                                                                                                                    TTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAG  >  SRR19973062.4246454/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TATTGGTGCATCAGGTGAAGTGAGCGCAGTGGAGACAATTTCATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAG  >  CP009273/4162968‑4163265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑