Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,132,494 T→A 40.3% I255I (ATT→ATA)  flgI → putative flagellar basal body protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,132,4940TA40.3% 182.5 / inf 246I255I (ATT→ATA) flgIputative flagellar basal body protein
Reads supporting (aligned to +/- strand):  ref base T (88/59);  new base A (44/55);  total (132/114)
Fisher's exact test for biased strand distribution p-value = 1.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01

Only 100 of 250 total aligned reads displayed.

GCGTGCGTGGATATGGCAGCGCCACCGCGTTAGATGCGCGGACTATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTAC  >  CP009273/1132346‑1132643
                                                                                                                                                    |                                                                                                                                                     
  gtgcgtgGATATGGCAGCGCCACCGCGTTAGATGCGCGGACTATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAAc                                                                                                                                                    <  2:1311618/150‑1 (MQ=255)
         gATATGGCAGCGCCACCGCGTTAGATGCGCGGACTATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCa                                                                                                                                             <  2:1773369/150‑1 (MQ=255)
           tatGGCAGCGCCACCGCGTTAGATGCGCGGACTATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCAcc                                                                                                                                           >  2:3390175/1‑150 (MQ=255)
           tatGGCAGCGCCACCGCGTTAGATGCGCGGACTATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCAcc                                                                                                                                           <  2:3611195/150‑1 (MQ=255)
             tGGCAGCGCCACCGCGTTAGATGCGCGGACTATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCgg                                                                                                                                         >  1:1119710/1‑150 (MQ=255)
              ggCAGCGCCACCGCGTTAGATGCGCGGACTATTCATGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGATAAAGTAGTGATTAACTCGCGCACCGGt                                                                                                                                        >  1:561610/1‑150 (MQ=255)
              ggCAGCGCCACCGCGTTAGATGCGCGGACTATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGt                                                                                                                                        >  2:1630176/1‑150 (MQ=255)
              ggCAGCGCCACCGCGTTAGATGCGCGGACTATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGt                                                                                                                                        <  1:2545094/150‑1 (MQ=255)
                 aGCGCCACCGCGTTAGATGCGCGGACTATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCg                                                                                                                                     <  2:3351445/150‑1 (MQ=255)
                 aGCGCCACCGCGTTAGATGCGCGGACTATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCg                                                                                                                                     <  2:1591481/150‑1 (MQ=255)
                 aGCGCCACCGCGTTAGATGCGCGGACTATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCg                                                                                                                                     <  2:841434/150‑1 (MQ=255)
                 aGCGCCACCGCGTTAGATGCGCGGACTATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCGTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCg                                                                                                                                     <  2:783474/150‑1 (MQ=255)
                     ccACCGCGTTAGATGCGCGGACTATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCggtgg                                                                                                                                 <  1:2665555/150‑1 (MQ=255)
                     ccACCGCGTTAGATGCGCGGACTATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCACAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCggtgg                                                                                                                                 >  1:2042006/1‑150 (MQ=255)
                      cACCGCGTTAGATGCGCGGACTATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCggtggt                                                                                                                                <  1:3890997/150‑1 (MQ=255)
                               aGATGCGCGGACTATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATcg                                                                                                                       <  2:4170100/150‑1 (MQ=255)
                               aGATGCGCGGACTATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATcg                                                                                                                       >  2:275603/1‑150 (MQ=255)
                                 aTGCGCGGACTATTCAGGTGCGCGTACTGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATcgcg                                                                                                                     <  1:3410946/150‑1 (MQ=255)
                                            aTTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTc                                                                                                          >  2:2388630/1‑150 (MQ=255)
                                             ttCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCg                                                                                                         <  2:1835538/150‑1 (MQ=255)
                                                aGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACa                                                                                                      >  2:2268157/1‑150 (MQ=255)
                                                  gTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGc                                                                                                    <  1:999039/150‑1 (MQ=255)
                                                  gTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGc                                                                                                    >  1:433305/1‑150 (MQ=255)
                                                  gTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGc                                                                                                    >  1:433301/1‑150 (MQ=255)
                                                     cgcgTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTgc                                                                                                 <  2:574918/150‑1 (MQ=255)
                                                       cgTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTgcgc                                                                                               >  2:1470179/1‑150 (MQ=255)
                                                       cgTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTgcgc                                                                                               <  2:2230036/150‑1 (MQ=255)
                                                            cGAGTGGCAACAGTTCCCAGGTCCGCGTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAg                                                                                          <  2:2262845/150‑1 (MQ=255)
                                                                 ggCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAg                                                                                     <  1:2439235/150‑1 (MQ=255)
                                                                  gCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAgg                                                                                    >  1:356454/1‑150 (MQ=255)
                                                                   cAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAggg                                                                                   >  1:1302388/1‑150 (MQ=255)
                                                                    aaCAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAgggg                                                                                  >  1:3655982/1‑150 (MQ=255)
                                                                     aCAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGa                                                                                 <  1:1961387/150‑1 (MQ=255)
                                                                           cccAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAAtctct                                                                           >  1:794955/1‑150 (MQ=255)
                                                                           cccAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAAtctct                                                                           >  1:3934079/1‑150 (MQ=255)
                                                                            ccAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAAtctctc                                                                          <  2:3520204/150‑1 (MQ=255)
                                                                             cAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCa                                                                         >  2:3013272/1‑150 (MQ=255)
                                                                              aGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTAGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAg                                                                        >  1:795023/1‑150 (MQ=255)
                                                                              aGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAg                                                                        <  1:1363255/150‑1 (MQ=255)
                                                                                         cTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAAt                                                             >  2:3313091/1‑150 (MQ=255)
                                                                                         cTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAAt                                                             <  1:2232182/150‑1 (MQ=255)
                                                                                          ttGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAAta                                                            >  1:2620381/1‑149 (MQ=255)
                                                                                           tGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCg                                                           <  2:3465241/150‑1 (MQ=255)
                                                                                            gCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGt                                                          >  2:649304/1‑150 (MQ=255)
                                                                                            gCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGt                                                          >  1:1076707/1‑150 (MQ=255)
                                                                                             ccGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTc                                                         <  1:2720755/150‑1 (MQ=255)
                                                                                              cGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCa                                                        >  2:2679242/1‑150 (MQ=255)
                                                                                                  atTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGcc                                                    >  1:3801010/1‑150 (MQ=255)
                                                                                                   ttCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCa                                                   >  1:671623/1‑150 (MQ=255)
                                                                                                    tCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCaa                                                  >  2:882259/1‑150 (MQ=255)
                                                                                                     cAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAAt                                                 >  1:2352022/1‑150 (MQ=255)
                                                                                                      aGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATg                                                >  2:1198691/1‑150 (MQ=255)
                                                                                                       gAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGt                                               >  2:1329082/1‑150 (MQ=255)
                                                                                                       gAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGt                                               >  2:3068860/1‑150 (MQ=255)
                                                                                                        aaTATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTc                                              >  2:3923856/1‑150 (MQ=255)
                                                                                                         atatGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTca                                             <  2:2529078/150‑1 (MQ=255)
                                                                                                          tatGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTcag                                            >  1:3484744/1‑150 (MQ=255)
                                                                                                           atGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTcagc                                           >  2:2537965/1‑150 (MQ=255)
                                                                                                            tGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTcagcc                                          <  2:3007510/150‑1 (MQ=255)
                                                                                                             gCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTcagcca                                         <  1:2891329/150‑1 (MQ=255)
                                                                                                                 gTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAgccagcca                                     >  1:877908/1‑150 (MQ=255)
                                                                                                                 gTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAgccagcca                                     >  1:1872557/1‑150 (MQ=255)
                                                                                                                   tAATTTCACCCCGCAGGACGCAAAAGTAGTTATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGAGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGTGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGa                                   >  2:2435125/1‑150 (MQ=255)
                                                                                                                   tAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGa                                   <  2:1369733/150‑1 (MQ=255)
                                                                                                                    aaTGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGAt                                  <  1:2410280/150‑1 (MQ=255)
                                                                                                                      tGTCACCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATac                                <  1:1232775/150‑1 (MQ=255)
                                                                                                                        tCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATacac                              >  2:3312566/1‑150 (MQ=255)
                                                                                                                          aCCCCGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCg                            >  2:1139729/1‑150 (MQ=255)
                                                                                                                            cccGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGtt                          >  1:721544/1‑150 (MQ=255)
                                                                                                                            cccGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGtt                          >  1:1608590/1‑150 (MQ=255)
                                                                                                                             ccGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGttt                         <  1:4167538/150‑1 (MQ=255)
                                                                                                                             ccGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGttt                         <  1:3093808/150‑1 (MQ=255)
                                                                                                                              cGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTtg                        <  1:175623/150‑1 (MQ=255)
                                                                                                                              cGCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTtg                        >  1:2342243/1‑150 (MQ=255)
                                                                                                                               gCAGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTtgg                       <  2:4093241/150‑1 (MQ=255)
                                                                                                                                cAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTtggt                      >  1:3203453/1‑150 (MQ=255)
                                                                                                                                cAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTtggt                      <  1:1928212/150‑1 (MQ=255)
                                                                                                                                 aGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTtggtg                     >  1:1697126/1‑150 (MQ=255)
                                                                                                                                 aGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTtggtg                     <  2:2698724/150‑1 (MQ=255)
                                                                                                                                 aGGACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTtggtg                     <  2:2698744/150‑1 (MQ=255)
                                                                                                                                   gACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTtggtggt                   >  1:1434815/1‑150 (MQ=255)
                                                                                                                                   gACGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTtggtggt                   >  1:2192748/1‑150 (MQ=255)
                                                                                                                                    aCGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTtggtggtg                  <  2:3650048/150‑1 (MQ=255)
                                                                                                                                    aCGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTtggtggtg                  <  1:3385659/150‑1 (MQ=255)
                                                                                                                                    aCGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTtggtggtg                  <  1:869250/150‑1 (MQ=255)
                                                                                                                                    aCGCTAAAGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTtggtggtg                  <  1:2953323/150‑1 (MQ=255)
                                                                                                                                      gCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGa                <  2:561610/150‑1 (MQ=255)
                                                                                                                                        tAAAGTAGTTATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACa              >  1:2970664/1‑150 (MQ=255)
                                                                                                                                         aaaGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAg             <  2:1403185/150‑1 (MQ=255)
                                                                                                                                          aaGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGa            <  2:1459463/150‑1 (MQ=255)
                                                                                                                                          aaGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGa            <  2:1233991/150‑1 (MQ=255)
                                                                                                                                          aaGTAGTGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGa            <  2:1233704/150‑1 (MQ=255)
                                                                                                                                           attagtGATTAACTCGCGCACCGTTTCGGTGGTGTTGTTTCGCGAAGTGACCCTCTACAGCTGCGCGGTAGCTCAGGGGAATGTCTCAGTAACAGTTAATCGTCAGGCAAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGAc           <  1:3778990/148‑1 (MQ=255)
                                                                                                                                           agtagtGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGAc           <  2:1210371/150‑1 (MQ=255)
                                                                                                                                            gtagtGATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACt          <  2:2391485/150‑1 (MQ=255)
                                                                                                                                                tGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTgg      >  1:2706305/1‑150 (MQ=255)
                                                                                                                                                tGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTgg      <  2:2145773/150‑1 (MQ=255)
                                                                                                                                                 gATAAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGt     <  2:3964911/150‑1 (MQ=255)
                                                                                                                                                  aTTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGtt    <  1:516636/150‑1 (MQ=255)
                                                                                                                                                   ttAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTa   >  1:1575421/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCGTGCGTGGATATGGCAGCGCCACCGCGTTAGATGCGCGGACTATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTAC  >  CP009273/1132346‑1132643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A