Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 766,563 G→A 26.0% intergenic (+496/‑351) mngB → / → cydA alpha‑mannosidase/cytochrome d terminal oxidase, subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273766,5630GA26.0% 179.2 / 69.2 96intergenic (+496/‑351)mngB/cydAalpha‑mannosidase/cytochrome d terminal oxidase, subunit I
Reads supporting (aligned to +/- strand):  ref base G (29/42);  new base A (16/9);  total (45/51)
Fisher's exact test for biased strand distribution p-value = 6.25e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

AATTAAGGTCAACCGTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTAT  >  CP009273/766417‑766711
                                                                                                                                                  |                                                                                                                                                    
aaTTAAGGTCAACCGTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAAt                                                                                                                                                   <  2:3381237/150‑1 (MQ=255)
 aTTAAGGTCAACCGTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAAtt                                                                                                                                                  >  2:3235981/1‑150 (MQ=255)
    aaGGTCAACCGTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGat                                                                                                                                               <  1:2447996/150‑1 (MQ=255)
     aGGTCAACCGTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATATCTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGata                                                                                                                                              <  1:1749175/150‑1 (MQ=255)
     aGGTCAACCGTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGata                                                                                                                                              <  1:503037/150‑1 (MQ=255)
     aGGTCAACCGTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGata                                                                                                                                              <  1:502571/150‑1 (MQ=255)
              gTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAAt                                                                                                                                     >  1:1781212/1‑150 (MQ=255)
              gTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAAt                                                                                                                                     >  1:3343599/1‑150 (MQ=255)
              gTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACAAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAAt                                                                                                                                     >  1:903977/1‑150 (MQ=255)
                 cTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGta                                                                                                                                  <  2:790140/150‑1 (MQ=255)
                 cTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGta                                                                                                                                  >  2:1850080/1‑150 (MQ=255)
                  tGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGtat                                                                                                                                 <  2:942328/150‑1 (MQ=255)
                  tGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGtat                                                                                                                                 <  2:3663069/150‑1 (MQ=255)
                     ttttGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAg                                                                                                                              <  1:2564055/150‑1 (MQ=255)
                        tGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGtat                                                                                                                           >  2:4425432/1‑148 (MQ=255)
                        tGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCt                                                                                                                           >  2:4422883/1‑150 (MQ=255)
                          cTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTg                                                                                                                         <  1:377442/150‑1 (MQ=255)
                               tctcTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAAt                                                                                                                    >  2:1455241/1‑150 (MQ=255)
                                ctctTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATg                                                                                                                   <  2:2690316/150‑1 (MQ=255)
                                ctctTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATg                                                                                                                   <  2:4047709/150‑1 (MQ=255)
                                  ctTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATggg                                                                                                                 <  1:3254527/150‑1 (MQ=255)
                                   tttttATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGc                                                                                                                >  2:3279404/1‑150 (MQ=255)
                                     tttATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCAt                                                                                                              >  1:4715784/1‑150 (MQ=255)
                                       tATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCa                                                                                                            >  1:4354946/1‑150 (MQ=255)
                                        aTCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCaa                                                                                                           <  1:3379226/150‑1 (MQ=255)
                                        aTCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCaa                                                                                                           <  2:3689531/150‑1 (MQ=255)
                                        aTCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCaa                                                                                                           <  2:3688237/150‑1 (MQ=255)
                                        aTCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCaa                                                                                                           <  2:3688053/150‑1 (MQ=255)
                                           tttAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCaaaaa                                                                                                        >  2:2075492/1‑150 (MQ=255)
                                            ttAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAag                                                                                                       >  2:590026/1‑150 (MQ=255)
                                             tAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAaga                                                                                                      >  1:3930988/1‑150 (MQ=255)
                                                ttGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGAt                                                                                                   <  1:3435996/150‑1 (MQ=255)
                                                ttGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGAt                                                                                                   <  2:3766750/150‑1 (MQ=255)
                                                 tGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATa                                                                                                  <  2:4074367/150‑1 (MQ=255)
                                                 tGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATa                                                                                                  <  2:249088/150‑1 (MQ=255)
                                                    cAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAAt                                                                                               <  1:3480353/150‑1 (MQ=255)
                                                       ccGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGt                                                                                            <  1:2483761/150‑1 (MQ=255)
                                                       ccGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGt                                                                                            <  1:347692/150‑1 (MQ=255)
                                                         gAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTtc                                                                                          >  1:3234718/1‑150 (MQ=255)
                                                            aCTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCg                                                                                       <  1:4540876/150‑1 (MQ=255)
                                                             cTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGa                                                                                      >  2:72909/1‑150 (MQ=255)
                                                             cTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGa                                                                                      >  2:74518/1‑150 (MQ=255)
                                                              tAATTTCAGCCTTATAACTCCCACATATTAAACATAACTGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGAt                                                                                     <  1:2131153/150‑1 (MQ=255)
                                                               aaTTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATc                                                                                    >  1:1047930/1‑150 (MQ=255)
                                                                aTTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCa                                                                                   <  2:3040829/150‑1 (MQ=255)
                                                                     aGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTg                                                                              <  1:3547752/150‑1 (MQ=255)
                                                                     aGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTg                                                                              <  1:3547751/150‑1 (MQ=255)
                                                                                tCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCg                                                                   <  2:470718/150‑1 (MQ=255)
                                                                                tCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCg                                                                   <  2:470828/150‑1 (MQ=255)
                                                                                    acaTTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTaa                                                               <  1:3614532/150‑1 (MQ=255)
                                                                                      aTTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAAtt                                                             <  2:639374/150‑1 (MQ=255)
                                                                                        tttAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTa                                                           <  1:3815990/150‑1 (MQ=255)
                                                                                        tttAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTa                                                           >  1:843464/1‑150 (MQ=255)
                                                                                             aCATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCtat                                                      >  1:197268/1‑150 (MQ=255)
                                                                                              cATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCtata                                                     <  1:2748771/150‑1 (MQ=255)
                                                                                                tAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATaaa                                                   >  2:1649020/1‑150 (MQ=255)
                                                                                                tAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATaaa                                                   >  1:536143/1‑150 (MQ=255)
                                                                                                  aaTGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTGGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAAtt                                                 >  2:2308366/1‑150 (MQ=255)
                                                                                                   aTGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTg                                                >  2:2114801/1‑150 (MQ=255)
                                                                                                    tGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGa                                               >  1:863904/1‑150 (MQ=255)
                                                                                                     gTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGAt                                              >  2:2401477/1‑150 (MQ=255)
                                                                                                      tCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATc                                             >  1:2206086/1‑150 (MQ=255)
                                                                                                         cTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCAcc                                          <  1:3124537/150‑1 (MQ=255)
                                                                                                          tAAAGTTACCTTATTGAAGCATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCg                                         <  1:1095297/150‑1 (MQ=255)
                                                                                                                tACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGaaa                                   >  2:3075331/1‑150 (MQ=255)
                                                                                                                   cTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAAt                                <  1:2563510/150‑1 (MQ=255)
                                                                                                                   cTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAAt                                <  2:2223352/150‑1 (MQ=255)
                                                                                                                   cTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAAt                                <  2:1250277/150‑1 (MQ=255)
                                                                                                                    ttATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAAta                               <  1:4384719/150‑2 (MQ=255)
                                                                                                                    ttATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAAta                               <  1:2321749/150‑2 (MQ=255)
                                                                                                                    ttATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATg                               <  2:2079982/150‑1 (MQ=255)
                                                                                                                      aTTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCa                             >  2:31861/1‑150 (MQ=255)
                                                                                                                      aTTGAAACATGATTAACATAATATGTAGGAATTGATAATTATCAATGTAAAAGTCTTTGAAATCGGCATCAAAAAGAGATAAAATAAACTCGATCAAATTGGCTGAAATGCGGTAATAAAGATATAAATACATCACCGTCGAAAAATgaa                             >  1:1408780/1‑148 (MQ=255)
                                                                                                                        tGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCaaa                           <  2:1494720/150‑1 (MQ=255)
                                                                                                                        tGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCaaa                           <  2:3955399/150‑1 (MQ=255)
                                                                                                                         gAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAAt                          <  1:929420/150‑1 (MQ=255)
                                                                                                                         gAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAAt                          <  1:929521/150‑1 (MQ=255)
                                                                                                                         gAAACATGATTAACATAAGTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTCATTTAGCTATAAATTGATCACCGTCGAAAACTGCAAAt                          >  2:3155984/1‑150 (MQ=255)
                                                                                                                          aaaCATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAAtt                         >  1:2982456/1‑150 (MQ=255)
                                                                                                                          aaaCATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATAGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAAtt                         >  1:2980913/1‑150 (MQ=255)
                                                                                                                          aaaCATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAAtt                         >  1:1919745/1‑150 (MQ=255)
                                                                                                                          aaaCATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAAtt                         >  1:1919748/1‑150 (MQ=255)
                                                                                                                            aCATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTg                       <  1:4847511/150‑1 (MQ=255)
                                                                                                                              aTGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCt                     >  2:3735752/1‑150 (MQ=255)
                                                                                                                                gATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTc                   <  2:1742602/150‑1 (MQ=255)
                                                                                                                                gATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTc                   >  1:2920594/1‑150 (MQ=255)
                                                                                                                                gATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTc                   >  1:489295/1‑150 (MQ=255)
                                                                                                                                    aaCATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTcaaca               >  2:844385/1‑150 (MQ=255)
                                                                                                                                    aaCATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTcaaca               >  2:3420722/1‑150 (MQ=255)
                                                                                                                                    aaCATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTcaaca               >  2:3505964/1‑150 (MQ=255)
                                                                                                                                         aaTTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAAc          <  1:1166376/150‑1 (MQ=255)
                                                                                                                                             tGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGt      >  1:4034876/1‑150 (MQ=255)
                                                                                                                                               tAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGttt    >  2:2783286/1‑150 (MQ=255)
                                                                                                                                               tAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGttt    >  2:1813822/1‑150 (MQ=255)
                                                                                                                                               tAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGttt    >  2:1813123/1‑150 (MQ=255)
                                                                                                                                                aGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTa   <  1:2412523/150‑1 (MQ=255)
                                                                                                                                                 ggAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTAt  >  1:898787/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AATTAAGGTCAACCGTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTAT  >  CP009273/766417‑766711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A